CN107668314A - A kind of industrialized production pea active peptide and preparation method - Google Patents
A kind of industrialized production pea active peptide and preparation method Download PDFInfo
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- CN107668314A CN107668314A CN201711068356.9A CN201711068356A CN107668314A CN 107668314 A CN107668314 A CN 107668314A CN 201711068356 A CN201711068356 A CN 201711068356A CN 107668314 A CN107668314 A CN 107668314A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The present invention discloses a kind of method of industrialized production pea active peptide.The present invention is digested using pea as raw material using flavor, papain and trypsase multienzyme, and then by processes such as enzyme deactivation, centrifugation, filtering, concentration, decolouring and spray drying, pea active peptide is made.Component of its molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and tripeptides tyrosine glutamine tyrosine (Tyr Gln Tyr, YQY) sequence peptide fragment at least containing 2.0% content.The production method of the present invention has bioactivity height, processing cost is low, treating capacity is big, is easy to the advantages of industrialized production, the pea active peptide prepared in this approach can be applied to the exploitation and production of ordinary food, health food and medicine as new functional nutrient composition, have wide market prospects.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of industrialized production pea active peptide and preparation method.
Background technology
Pea, it is that year life climber herbaceous plant is got in the annual or autumn sowing of spring sowing, is gained the name because of its climbing up by holding on to property of stalk.Circle body
Also known as honey pea or sweet beans.Pea has multiple functions, such as strengthens body's immunity, each of needed by human body is rich in pea
Kind nutriment, especially contains good protein, can improve the resistance against diseases and rehabilitation ability of body.Cancer preventing and treating：Pea
Carrotene is rich in beans, the synthesis of human carcinogen's material can be prevented after edible, so as to reduce the formation of cancer cell, reduces human body
The incidence of disease of cancer.Tonneau large intestine：Crude fibre is rich in pea, intestinal peristalsis can be promoted, keeps stool smooth, it is big to play cleaning
The effect of intestines.Dietary function pea is sweet, mild-natured, returns spleen, stomach；With gas in benefit, antidiarrheal dysentery, adjust battalion to defend, diuresis, disappear carbuncle
Swell, solve the effect of mammary calculus poison；Cure mainly tinea pedis, carbuncle swells, alactation, taste discomfort, hiccup vomiting, trusted subordinate's distending pain, thirsty let out dysentery
Etc. illness.
By carrying out protease hydrolyzed to peameal, the oligopeptide by proteolysis into small-molecular-weight, production is considerably increased
The dissolubility of product, the success of pea active peptide are prepared as traditional food peameal deep processing of farm products and provide an infant industry
Change road, this abundant ordinary food protein resource of peameal is fully used, expand peameal significantly as one
The application field of the traditional ordinary food raw material of kind, improves the added value of large agricultural product, has played bigger economic value, and
Improve comprehensive social benefit.Compared with former albumen, the pea active peptide of small molecule is more prone to be digested absorption.Due to it
A series of good food processing properties, pea active peptide can also mix with the protein of other separate sources, to improve its battalion
Support value, it can also be used to produce the diversified food such as instant drink, drinks, meat products, dairy products, bakery product.Pea
Beans active peptide can be added to many because of its at a relatively high security and nutritive value, and with good food processing properties
Food in the middle of, its market prospects is very good.
The content of the invention
Present invention aims at a kind of preparation method of industrialized production pea active peptide is provided, to solve technical problem.
A kind of preparation method of pea active peptide, comprises the following steps：
A, pea is pre-processed, carries out ultramicro grinding, 200-600 mesh is crossed, with 100: 2~10 (L: kg) liquid ratio water
Sized mixing with peameal mixing, adjust the micro- basifications of pH 8-10, be heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 60~80 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4-6, mechanical agitation 8-12h, centrifugation, collects precipitation, precipitation as pea egg
In vain, it is repeated twice；
D, precipitation plus water are redissolved, is heated to 75-100 DEG C, time 15-45min so that protein denaturation, then carry out
It is high-pressure homogeneous；
E, above-mentioned pH value of solution is adjusted to 5-10, adds special enzyme preparation: flavor protease: papain: trypsase
For 1~3: 2~4: 1~2, hydrolysis temperature is 40-65 DEG C, time 1.5-8h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into pea
Active peptide powder.
The preparation method of pea active peptide described above, it is characterised in that in described step A, carrying out ultramicro grinding can
To improve enzymatic hydrolyzation, material composition is made full use of.
The preparation method of pea active peptide described above, it is characterised in that in described step C, using the side of thermal denaturation
Method, it can be more favorable for digesting, enzymolysis is more abundant.High-pressure homogeneous, further raising enzymolysis effect is carried out after thermal denaturation simultaneously
Rate.
The preparation method of pea active peptide described above, it is characterised in that described step D, special enzyme preparation combination bag
Include flavor protease: papain: trypsase is 1~3: 2~4: 1~2.Research finds the enzyme of its special enzyme preparation combination
Solve the enzymolysis efficiency of the long-range about single enzyme of efficiency.
The preparation method of pea active peptide described above, it is characterised in that being done for middle cold nebulization used in being spray-dried
Dry tower, further retain thermal activity material, and middle low-temperature spray drying tower is to be applied first in peptide technique.
A kind of preparation method of pea active peptide, it is characterised in that described according to the preparation method described in claim 1
Pea oligopeptide is characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and
Tripeptides Tyr-Gln-tyrosine (Tyr-Gln-Tyr, YQY) sequence peptide fragment at least containing 2.0% content.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
Embodiments of the invention are described in detail below, but the present invention can be limited and covered according to claim
Multitude of different ways implement.
1st, a kind of preparation method of industrialized production pea active peptide, comprises the following steps：
A, pea is pre-processed, carries out ultramicro grinding, 200-600 mesh is crossed, with 100: 7 (L: kg) liquid ratio water and pea
Bean powder mixing is sized mixing, and is adjusted 9 micro- basifications of pH, is heated to 70 DEG C.Insulated and stirred 80min；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 70 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 5, mechanical agitation 10h, centrifugation, collects precipitation, precipitation is pea protein, heavy
Again twice；
D, precipitation plus water are redissolved, is heated to 85 DEG C, time 30min so that protein denaturation, it is equal then to enter horizontal high voltage
Matter；
E, above-mentioned pH value of solution is adjusted to 6, adds special enzyme preparation: flavor protease: papain: trypsase is
2.5: 3.5: 1.5, flavor protease: papain: the addition of trypsase is every gram of unit of protein 1500, enzymolysis temperature
Spend for 50 DEG C, time 3h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：To centrifuge, filtering by thick enzymolysis liquid, filtered using 0.05~0.1 μm of the membrane filter system in aperture, condition
For 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.It is concentrated by evaporation, adds activated carbon decolorizing, adds concentrate total solids content
5% quality of activated carbon is decolourized, and then spray drying obtains pea active peptide powder.
2nd, the physics and chemistry composition of pea active peptide and molecular weight distribution analysis
The composition analysis result of pea active peptide is shown in Table 1, it can be seen that the total egg of pea active peptide prepared by the present invention
White matter content is up to 97.5%, has very high product quality.
Table 1：The basic physical and chemical composition analysis of pea active peptide
| Content % | |
| Gross protein (butt) | 97.5±0.32 |
| Fat | 0.09±0.01 |
| Ash content | 4.13±0.13 |
Pea active peptide sample after sample introduction, is obtained its gel chromatography figure, handled with liquid data in GEL-HPLC
The gel chromatography data of pea active peptide are substituted into calibration curve equation and calculated by software, obtain relative point of the peptide of sample
Protonatomic mass and its distribution.The absorption rate pole in human body of dipeptides, tripeptides in molecular weight 1000Da following components
Height, there is the higher nutritive value of specific ionization amino acid and physiological function.The present invention calculates pea with areas of peak normalization method
The relative molecular mass distribution scope of active peptide, as shown in table 2.Molecular weight is less than it can be seen from molecular weight distribution result
1000Da component accounts for 91.62% altogether, if calculated by the mean molecule quantity 137Da of amino acid, molecular weight 1000Da with
Under component it is then mostly be oligopeptide below octapeptide, also including part free amino acid.Its middle-molecular-weihydroxyethyl is 140Da-500Da's
59.97% is accounted for, occupies below the 1000Da overwhelming majority, this part is mainly dipeptides, tripeptides and tetrapeptide.
Table 2：Pea bioactive peptide molecule amount distribution results
3rd, Structural Identification quantifies with significant peptide fragment in pea active peptide
Shown according to domestic and international newest nutrient research, after protein intake, be not fully hydrolyzed into amino acid, but big portion
Divide and digested and assimilated in the form of peptide by body.Peptide matters are in addition to trophic function that is easy to digest, easily absorbing, prior life
Thing meaning, which is mainly reflected in it, has the incomparable physiological active functionses of amino acid.Pea active peptide is as a kind of Combination
Peptide, contain many peptide fragments with various physiological functions.Meanwhile its relative molecular mass less than 1000 component account for 90% with
On, illustrate that this product is substantially made up of small peptide.Special protein raw materials source and unique production technology, contain this product
There is a certain amount of characteristic component peptide.
The peptide fragment in pea active peptide is separated for we and Structural Identification.By using high performance liquid chromatography separation with
Mass spectral analysis, is separated and Structural Identification to the peptide fragment in this product, identifies the primary structure of 38 peptide fragments altogether.
We have worked out tripeptides Tyr-Gln-tyrosine (Tyr-Gln- in quantitative determination pea active peptide
Tyr, YQY) high efficiency liquid phase chromatographic analysis method.By pea active peptide sample after pre-treatment, using anti-phase C18 fillers as fixation
Phase, the difference according to sample component molecular polarity size are separated, detected under the conditions of UV absorption wavelength 220nm, are used
External standard method is quantified, and chromatogram and its data are handled, and YQY content is calculated.In view of the pea of different batches
Containing more than 2.0% YQY in beans active peptide sample, therefore using YQY as the characteristic chemical constituent in pea active peptide.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (6)
1. a kind of preparation method of industrialized production pea active peptide, it is characterised in that comprise the following steps：
A, pea is pre-processed, carries out ultramicro grinding, cross 200~600 mesh, with 100: 2~10 (L: kg) liquid ratio water and
Peameal mixing is sized mixing, and is adjusted the micro- basification in pH8~10, is heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, slag charge plus water is dilute
Release, be heated to 60~80 DEG C, stir and separate, carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4~6,8~12h of mechanical agitation, centrifugation, is collected precipitation, is precipitated as pea protein,
It is repeated twice；
D, precipitation plus water are redissolved, is heated to 75~100 DEG C, 15~45min of time so that protein denaturation, then carried out high
Press homogeneous；
E, above-mentioned pH value of solution is adjusted to 5~10, adds special enzyme preparation：Flavor protease: papain: trypsase 1
~3: 2~4: 1~2, hydrolysis temperature is 40~65 DEG C, and the time is 1.5~8h；
F, after enzymolysis terminates, 90~125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1~3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into pea activity
Gly-His-Lys.
2. according to the method for claim 1, it is characterised in that flavor protease in the step E: papain: pancreas
The addition of protease is every gram of unit of protein 1000~2000.
3. according to the method for claim 1, it is characterised in that in the step G, using 0.05~0.1 μm of the film in aperture
Filter plant is filtered, and condition is 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.
4. according to the method for claim 1, it is characterised in that in the step G, add concentrate total solids content
5% quality of activated carbon is decolourized.
A kind of 5. preparation method of pea active peptide, it is characterised in that according to the preparation method described in claim 1, the pea
Beans oligopeptide is characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and extremely
The few tripeptides Tyr-Gln containing 2.0% content-tyrosine (Tyr-Gln-Tyr, YQY) sequence peptide fragment.
6. anti-hypertension is had as product or raw material according to pea active peptide prepared by any one of claim 1-4 methods described
Effect.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055466A (en) * | 2018-07-30 | 2018-12-21 | 山东理工大学 | A kind of preparation method of pea peptide and its application in whitening field |
| CN109907156A (en) * | 2019-03-19 | 2019-06-21 | 烟台东方蛋白科技有限公司 | A kind of production method reconstituting type pea separation protein finished product |
| CN109907155A (en) * | 2019-03-19 | 2019-06-21 | 烟台东方蛋白科技有限公司 | A kind of production technology reconstituting type pea separation protein powder |
| CN112176016A (en) * | 2020-10-14 | 2021-01-05 | 中肽生物科技研究院(山东)有限公司 | Preparation method of bean nano-scale small molecule composite peptide |
| CN112626155A (en) * | 2020-06-09 | 2021-04-09 | 南京泛成生物科技有限公司 | Preparation method of pea peptide |
| CN112725398A (en) * | 2019-10-29 | 2021-04-30 | 中国食品发酵工业研究院有限公司 | Pea peptide with auxiliary hypoglycemic function and preparation method thereof |
| CN113615839A (en) * | 2021-07-13 | 2021-11-09 | 安徽金源药业有限公司 | Composite probiotic high-calcium protein powder capable of enhancing immunity |
| CN113768031A (en) * | 2021-09-14 | 2021-12-10 | 临邑禹王植物蛋白有限公司 | Production method of pea protein with low content of volatile organic compounds |
| BE1028096B1 (en) * | 2020-03-09 | 2022-01-17 | Yantai Shuangta Food Co Ltd | Process for extracting antimicrobial peptides and albumin from pea processing waste water |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109055466A (en) * | 2018-07-30 | 2018-12-21 | 山东理工大学 | A kind of preparation method of pea peptide and its application in whitening field |
| CN109907156A (en) * | 2019-03-19 | 2019-06-21 | 烟台东方蛋白科技有限公司 | A kind of production method reconstituting type pea separation protein finished product |
| CN109907155A (en) * | 2019-03-19 | 2019-06-21 | 烟台东方蛋白科技有限公司 | A kind of production technology reconstituting type pea separation protein powder |
| CN112725398A (en) * | 2019-10-29 | 2021-04-30 | 中国食品发酵工业研究院有限公司 | Pea peptide with auxiliary hypoglycemic function and preparation method thereof |
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| BE1028096B1 (en) * | 2020-03-09 | 2022-01-17 | Yantai Shuangta Food Co Ltd | Process for extracting antimicrobial peptides and albumin from pea processing waste water |
| CN112626155A (en) * | 2020-06-09 | 2021-04-09 | 南京泛成生物科技有限公司 | Preparation method of pea peptide |
| CN112176016A (en) * | 2020-10-14 | 2021-01-05 | 中肽生物科技研究院(山东)有限公司 | Preparation method of bean nano-scale small molecule composite peptide |
| CN113615839A (en) * | 2021-07-13 | 2021-11-09 | 安徽金源药业有限公司 | Composite probiotic high-calcium protein powder capable of enhancing immunity |
| CN113768031A (en) * | 2021-09-14 | 2021-12-10 | 临邑禹王植物蛋白有限公司 | Production method of pea protein with low content of volatile organic compounds |
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Application publication date: 20180209 |