CN107653186A - A kind of portable PCR instrument - Google Patents
A kind of portable PCR instrument Download PDFInfo
- Publication number
- CN107653186A CN107653186A CN201710833628.3A CN201710833628A CN107653186A CN 107653186 A CN107653186 A CN 107653186A CN 201710833628 A CN201710833628 A CN 201710833628A CN 107653186 A CN107653186 A CN 107653186A
- Authority
- CN
- China
- Prior art keywords
- moving cell
- test tube
- plate
- synchronizing wheel
- heat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of portable PCR instrument, it includes：Heat-insulation unit, heating unit, optical detection unit, moving cell and control unit；Heating unit is used to the test tube be fixed, and the sample in test tube is heated, expanded the detected sample in the test tube, heat-insulation unit is used for test tube insulation and shading, optical detection unit is fixed on moving cell, control unit control moving cell switches between the horizontal movement of both direction, realizes that optical detection unit carries out optical detection to the detected sample in test tube.Portable PCR instrument provided by the invention, it is compact-sized compact, it is easy to carry, installing shell additional can not only use separately as instrument, can be also positioned over as a module in other modules.
Description
Technical field
The present invention relates to biology and analytical chemistry field, more particularly to a kind of portable PCR instrument.
Background technology
PCR (PCR) is life science, the important technology of medical domain, for being carried out greatly to target dna
Amount amplification.PCR instrument is the key instrument into performing PCR, is widely used in the cause of disease in diagnosing hereditary illness, detection clinical samples
The nucleic acid of body, make science of heredity identification etc. to legal medical expert's sample.Normal PCR analytical technology is put into sample using multi-hole orifice and carried out
Detection, required Biosample and reagent are more.At present, PCR instrument is mostly desktop computer, and body increasing is not readily portable, is not easy to existing
Field uses.
The content of the invention
The present invention is directed to above-mentioned problems of the prior art, proposes a kind of portable PCR instrument, one kind of the invention is just
Formula PCR instrument is taken, detection process is compact-sized compact without artificially participating in, and is easy to carry.The present invention is by following technical solution
Realize：
The present invention provides a kind of portable PCR instrument, it is characterised in that including：Heat-insulation unit, heating unit, optical detection
Unit, moving cell and control unit, wherein,
The heat-insulation unit is used for test tube insulation and shading；
The heating unit is used to the test tube be fixed, and the sample in test tube is heated, and makes the examination
The detected sample in pipe is expanded；
The optical detection unit is fixed on moving cell, for being carried out when the detected sample in the test tube
Optical detection；
Described control unit is connected with the moving cell, and the moving cell realizes the horizontal movement of both direction, institute
Control unit is stated to be used to control the moving cell to switch between the horizontal movement in described two directions.
It is preferred that the heat-insulation unit includes：Heat-insulation unit includes shell, heating plate, panel, and the heating plate is arranged on
In shell, heat is transmitted by shell.
The heating unit includes：Specimen holder, heating plate, heat-insulation layer and pressing plate.The heating plate, heat-insulation layer and pressure
Plate is pressed on specimen holder sequentially through screw, for the test tube to be fixed, and the sample in test tube is added
Heat；Specimen holder is arranged on support, and support is fixed by screws on bottom plate.
The moving cell includes：First moving cell and the second moving cell, the second moving cell are arranged at the first fortune
On the support plate of moving cell.First moving cell includes position sensor, motor, synchronizing wheel, guide rail, support plate, belt pressing plate, synchronization
Band, synchronous wheel support and synchronizing wheel；Guide rail is arranged on bottom plate, and one of synchronizing wheel is arranged on motor shaft, another
Synchronizing wheel is arranged on synchronous wheel support, and belt is installed on synchronizing wheel and upper, drives support plate to realize a side by belt drive
To horizontal movement, position sensor be arranged on bottom plate on detect this direction position.Second moving cell bag optical module support plate,
Guide rail, synchronous wheel support, synchronizing wheel, belt, belt pressing plate, motor, synchronizing wheel and position sensor；Guide rail is arranged on support plate
On；Optical module support plate is arranged on guide rail；Synchronous wheel support is arranged on support plate, installs synchronizing wheel thereon；Another is same
For step wheel on the axle of electrode, belt is installed on synchronizing wheel and upper, motor rotation, and one is realized by belt drive drive support plate
The horizontal movement in individual direction, position sensor are arranged on support plate and detect this direction position.Two moving cells realize two sides
To horizontal movement, scope covering specimen holder detection hole.
The optical detection unit is fixed on the optical module support plate of the second moving cell, for when in the test tube
The detected sample carries out optical detection；
Described control unit includes:Control panel and control board mount, control panel are arranged on control board mount, control panel branch
Frame is arranged on bottom plate.For controlling the moving cell to be switched between the horizontal movement in described two directions.
Compared to prior art, the present invention has advantages below：Portable PCR instrument provided by the invention is compact-sized small
Ingeniously, it is easy to carry, installing shell additional can not only use separately as instrument, also can be positioned over other modules as a module
It is interior.
Certainly, any product for implementing the present invention it is not absolutely required to reach all the above advantage simultaneously.
Brief description of the drawings
Embodiments of the present invention are described further below in conjunction with the accompanying drawings：
Fig. 1 is the structural representation of the portable PCR instrument of embodiments of the invention；
Fig. 2 is the structural representation of the heat-insulation unit of embodiments of the invention；
Fig. 3 is the structural representation of the heating unit of embodiments of the invention；
Fig. 4 is the sectional view of the moving cell of embodiments of the invention；
Fig. 5 is the structural representation of the moving cell of embodiments of the invention；
Label declaration：Heat-insulation unit 1a, heating unit 2a, optical detection unit 3a, moving cell 4a and control unit
5a.First moving cell 3a1 and the second moving cell 3a2.
Shell 1, heating plate 2, panel 3, specimen holder 4, heating plate 5, heat-insulation layer 6, pressing plate 7, support 8, position sensor 9,
Motor 10, synchronizing wheel 11, guide rail 12, support plate 13, belt pressing plate 14, timing belt 15, synchronous wheel support 16, synchronizing wheel 17, bottom plate
18, optical module support plate 19, guide rail 20, synchronous wheel support 21, synchronizing wheel 22, belt 23, belt pressing plate 24, motor 25, synchronization
Wheel 26, position sensor 27, control panel 28 and control board mount 29.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
With reference to Fig. 1-Fig. 5, the portable PCR instrument of the present invention is described in detail, its structural representation as shown in figure 1,
It includes：Heat-insulation unit 1a, heating unit 2a, optical detection unit 3a, moving cell 4a and control unit 5a.
The heat-insulation unit 1a as shown in Fig. 2 including：Heat-insulation unit includes shell 1, heating plate 2, panel 3, the heating
Plate is arranged in shell, transmits heat by shell one side, shell 1 is installed on panel 3 by bearing pin revolution.
As Fig. 3, the heating unit 2a include：Specimen holder 4, heating plate 5, heat-insulation layer 6 and pressing plate 7.The heating plate
5th, heat-insulation layer 6 and pressing plate 7 are pressed on specimen holder 4 sequentially through screw, for the test tube to be fixed, and to examination
Sample in pipe is heated；Specimen holder 4 is arranged on support 8, and support 8 is fixed by screws on bottom plate.
Such as Fig. 4 and Fig. 5, the moving cell 3a includes：First moving cell 3a1 and the second moving cell 3a2, the first fortune
Moving cell 3a1 includes position sensor 9, motor 10, synchronizing wheel 11, guide rail 12, support plate 13, belt pressing plate 14, timing belt 15, same
Walk wheel support 16 and synchronizing wheel 17；Guide rail sets 12 on bottom plate 18, and one of synchronizing wheel 11 is arranged on the axle of motor 10,
Another synchronizing wheel 17 is arranged on synchronous wheel support 16, and belt 15 is installed in synchronizing wheel 22 and 26, passes through the band of belt 15
The dynamic horizontal movement for driving support plate 14 to realize a direction, position sensor 9 are arranged on bottom plate 18 and detect this direction position.The
Two moving cell 3a2 bag optical modules support plates 19, guide rail 20, synchronous wheel support 21, synchronizing wheel 22, belt 23, belt pressing plate
24th, motor 25, synchronizing wheel 26 and position sensor 27；Guide rail 20 is arranged on support plate 13；Optical module support plate 19, which is arranged at, leads
On rail 20；Synchronous wheel support 21 is arranged on support plate 13, installs synchronizing wheel 22 thereon；Another synchronizing wheel 26 is arranged on electricity
On the axle of pole 25, belt 23 is installed in synchronizing wheel 22 and 26, and motor rotates, and drives support plate 24 to realize by belt 23
The horizontal movement in one direction, position sensor 27 are arranged on support plate 13 and detect this direction position.Two moving cells are realized
The horizontal movement of both direction, scope covering specimen holder detection hole.
The optical detection unit 4a is fixed on the second moving cell 3a2 optical module support plate 19, for when described
The detected sample in test tube carries out optical detection.
As Fig. 4, described control unit 5a are included：Control panel 28 and control board mount 29, control panel 28 are arranged on control panel
On support 29, control board mount 29 is arranged on bottom plate 18.For controlling the moving cell in the level in described two directions
Switched between motion.
Disclosed herein is only the preferred embodiments of the present invention, and this specification is chosen and specifically describes these embodiments, is
It is not limitation of the invention to preferably explain the principle and practical application of the present invention.Any those skilled in the art
The modifications and variations done in the range of specification, it all should fall in the range of the present invention protects.
Claims (6)
- A kind of 1. portable PCR instrument, it is characterised in that including：Heat-insulation unit, heating unit, optical detection unit, moving cell And control unit, wherein,The heat-insulation unit is used for test tube insulation and shading；The heating unit is used to the test tube be fixed, and the sample in test tube is heated, and makes in the test tube The detected sample expanded；The optical detection unit is fixed on moving cell, for carrying out optics when the detected sample in the test tube Detection；Described control unit is connected with the moving cell, and the moving cell realizes the horizontal movement of both direction, the control Unit processed is used to control the moving cell to switch between the horizontal movement in described two directions.
- 2. portable PCR instrument according to claim 1, it is characterised in that the heat-insulation unit includes：Heat-insulation unit includes Shell, heating plate, panel, the heating plate are arranged in shell, and heat is transmitted by shell one side, and providing top for test tube protects Temperature.
- 3. portable PCR instrument according to claim 1, it is characterised in that the heating unit includes：Specimen holder, heating Plate, heat-insulation layer and pressing plate.The heating plate, heat-insulation layer and pressing plate are pressed on specimen holder sequentially through screw, are used for The test tube is fixed, and the sample in test tube is heated；Specimen holder is arranged on support, and support is consolidated by screw It is scheduled on bottom plate.
- 4. portable PCR instrument according to claim 1, it is characterised in that the moving cell includes：First moving cell With the second moving cell, the second moving cell is arranged on the support plate of the first moving cell.
- 5. moving cell according to claim 4, it is characterised in that the first moving cell include position sensor, motor, Synchronizing wheel, guide rail, support plate, belt pressing plate, timing belt, synchronous wheel support and synchronizing wheel；Guide rail is arranged on bottom plate, one of them Synchronizing wheel is arranged on motor shaft, and another synchronizing wheel is arranged on synchronous wheel support, and belt is installed on synchronizing wheel and upper, is led to The horizontal movement that belt drive drives support plate to realize a direction is crossed, position sensor is arranged on bottom plate and detects this direction position Put.Second moving cell bag optical module support plate, guide rail, synchronous wheel support, synchronizing wheel, belt, belt pressing plate, motor, synchronization Wheel and position sensor；Guide rail is arranged on support plate；Optical module support plate is arranged on guide rail；Synchronous wheel support is arranged on support plate On, synchronizing wheel is installed thereon；Another synchronizing wheel is arranged on the axle of electrode, and belt is installed on synchronizing wheel and upper, motor turn It is dynamic, the horizontal movement in a direction is realized by belt drive drive support plate, position sensor is arranged on support plate and detects this side To position.Two moving cells realize the horizontal movement of both direction, scope covering specimen holder detection hole.
- 6. portable PCR instrument according to claim 1, it is characterised in that the optical detection unit is fixed on the second fortune On the optical module support plate of moving cell, for carrying out optical detection when the detected sample in the test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710833628.3A CN107653186A (en) | 2017-09-15 | 2017-09-15 | A kind of portable PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710833628.3A CN107653186A (en) | 2017-09-15 | 2017-09-15 | A kind of portable PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107653186A true CN107653186A (en) | 2018-02-02 |
Family
ID=61130092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710833628.3A Pending CN107653186A (en) | 2017-09-15 | 2017-09-15 | A kind of portable PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107653186A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628607A (en) * | 2019-10-10 | 2019-12-31 | 上海纽钛测控技术有限公司 | Efficient constant-temperature PCR device suitable for emergency field application and implementation method |
| WO2023178710A1 (en) * | 2022-03-23 | 2023-09-28 | 无锡百泰克生物技术有限公司 | Portable ultrafast real-time fluorescent quantitative pcr instrument |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800411A (en) * | 2005-01-04 | 2006-07-12 | 中国科学院光电技术研究所 | Thermal cycle control polymerase chain reaction biological detection system |
| CN101698823A (en) * | 2009-10-23 | 2010-04-28 | 西安天隆科技有限公司 | High-speed servo motor and optical positioning-based fluorescence quantitative PCR detection system |
| CN101952412A (en) * | 2008-02-15 | 2011-01-19 | 生物辐射实验室股份有限公司 | Thermal cycler with self-adjusting lid |
| US20120329058A1 (en) * | 2009-12-30 | 2012-12-27 | Huadong Researc Institute For Medicine And Biotechnics | Method and device for fast detecting nucleic acid |
| CN103534575A (en) * | 2011-02-04 | 2014-01-22 | 环球生物研究株式会社 | Automatic response/light measurement device and method therefor |
| CN204989024U (en) * | 2015-09-11 | 2016-01-20 | 广州迪澳生物科技有限公司 | Portable fluorescence detection device of constant temperature |
-
2017
- 2017-09-15 CN CN201710833628.3A patent/CN107653186A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800411A (en) * | 2005-01-04 | 2006-07-12 | 中国科学院光电技术研究所 | Thermal cycle control polymerase chain reaction biological detection system |
| CN101952412A (en) * | 2008-02-15 | 2011-01-19 | 生物辐射实验室股份有限公司 | Thermal cycler with self-adjusting lid |
| CN101698823A (en) * | 2009-10-23 | 2010-04-28 | 西安天隆科技有限公司 | High-speed servo motor and optical positioning-based fluorescence quantitative PCR detection system |
| US20120329058A1 (en) * | 2009-12-30 | 2012-12-27 | Huadong Researc Institute For Medicine And Biotechnics | Method and device for fast detecting nucleic acid |
| CN103534575A (en) * | 2011-02-04 | 2014-01-22 | 环球生物研究株式会社 | Automatic response/light measurement device and method therefor |
| CN204989024U (en) * | 2015-09-11 | 2016-01-20 | 广州迪澳生物科技有限公司 | Portable fluorescence detection device of constant temperature |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628607A (en) * | 2019-10-10 | 2019-12-31 | 上海纽钛测控技术有限公司 | Efficient constant-temperature PCR device suitable for emergency field application and implementation method |
| WO2023178710A1 (en) * | 2022-03-23 | 2023-09-28 | 无锡百泰克生物技术有限公司 | Portable ultrafast real-time fluorescent quantitative pcr instrument |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180202 |