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CN107410223A - A kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device automatically - Google Patents

A kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device automatically Download PDF

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Publication number
CN107410223A
CN107410223A CN201710748176.9A CN201710748176A CN107410223A CN 107410223 A CN107410223 A CN 107410223A CN 201710748176 A CN201710748176 A CN 201710748176A CN 107410223 A CN107410223 A CN 107410223A
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China
Prior art keywords
seedling
passage
electromagnetic shielding
magnetic field
pace
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CN201710748176.9A
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Chinese (zh)
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CN107410223B (en
Inventor
万贵钧
刘瑞莹
赵婧妤
张明
贺静澜
陈法军
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention discloses a kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing to monitor receipts sampling device, including electromagnetic shielding chamber automatically(26), in electromagnetic shielding chamber(26)It is provided with all the period of time core rotating table system, the rotating shaft of all the period of time core rotating table system(2)It is vertically arranged in electromagnetic shielding chamber(26)Middle part, along rotating shaft(2)Radially uniform distribution rotating disk cantilever(13)Distal end be fixed with pedestal fix support(31), part base, which is fixed, to be held in the palm(31)Top be provided with ultra-clear glasses raise worm cover(12), ultra-clear glasses feeding worm cover(12)Front and rear side be respectively equipped with and give seedling passage(14)With receipts seedling passage(19), the pedestal of remainder, which is fixed, to be held in the palm(31)Positioned at giving seedling passage(14)Tail end or positioned at receive seedling passage(19)Initiating terminal.The device of the present invention can use with any extraneous artificial magnetic field collocation, auto monitoring and measurement, receive sample and analytical behavior data, time saving and energy saving and accurate easily-controllable.

Description

A kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sample automatically Device
Technical field
The present invention relates to sample collection and monitoring technical field of auxiliary equipment, more particularly to a kind of animal physiological Behavior rhythm Automated watch-keeping facility, specifically a kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors automatically receives sample dress Put;The device, which utilizes, to be greatly saved manpower with reference to all the period of time core rotating table system to seedling receipts seedling system, improves efficiency;Utilize environment The factor controls greatly improves environmental stability and data readability with monitoring system and test worm activities monitoring system;Utilize electromagnetism Shielding cavity greatly reduces electromagnetic interference, improves magnetic field processing accuracy.
Background technology
Biological clock is a kind of invisible " clock " in organism, carries the internal yardstick of organism vital movement.Mesh It is preceding studied when reporting, the different Periodic biological clock such as day, week, month, year, imply biological physiology that biological clock dominates and Behavior rhythm may play a significant role in biological life activity.In physianthropy research, with reference to the border life of human body day The case of thing clock feature treating cancer, in addition, in pedagogy, also there is biological clock rhythm feature of the research according to different people because of material Teaching, brings notable results.In fact, showing at present researchs such as animal, plant, microorganisms, biological daily vital movement is invariably Specific rhythmicity is embodied, and for vector insect and crop pests, the rhythm and pace of moving things feature of its physiology and behavior is studied, Help to formulate efficient prevention and Management strategy according to the physiology and behavioral trait of its different time, reduce its harm.
Mainly there are artificial observational record and video recording observation combination soft for the research meanses of insect biological clock and biological rhythm Part analyzes two methods.Subordinate act research aspect, video recording observation with reference to software analysis method substantially can not expend compared with Meet experiment demand in the case of more manpowers.At present, binding molecule biology techniques, tentatively it is found that regulation and control biological rhythm Portion gene path, but for the research of similar deeper mechanisms, it usually needs by experimenter manually by when monitor and fixed When receive the basic means such as sample, in addition, the research for circadian rhythm is often also required to the similar manual operation of experimenter's progress.
In view of currently for the research aid for being related to circadian rhythm and sample timed collection in biological clock research, especially It is the cross discipline for becoming more and more important --- the versatile customization instrument of " biomagnetism " research is rarely reported, and relies on artificial receive Sample and the research method of observational record take time and effort, there may be human error, it would therefore be highly desirable to which it is accurate to develop the set of environmental factor The biological physiology Behavior rhythm that can be used for magnetic field processing controllable, that all the period of time sample is collected automatically, behavioral activity monitors in real time is ground Study carefully servicing unit.
The content of the invention
It is an object of the invention to:Fill up the existing insect physiological behavior rhythm and pace of moving things and respective sample and collect auxiliary automatically at times Device absence is the insect physiological behavior rhythm and pace of moving things to envirment factor response investigations, there is provided a set of to can be used for magnetic field processing, save people Power, efficient stable, envirment factor(Magnetic field, light source, humiture, gas characteristic)The integrated of controllable precise can be used at magnetic field The small insects physiological behavior rhythm and pace of moving things of reason monitors receipts sampling device automatically.
The purpose of the present invention solves by the following technical programs:
A kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device, including electromagnetic shielding chamber automatically, It is characterized in that:Described electromagnetic shielding chamber is provided with envirment factor control and monitoring system, test worm activities monitoring system, to seedling Seedling system and all the period of time core rotating table system are received, the rotating shaft of all the period of time core rotating table system is vertically arranged in electromagnetic shielding chamber Portion, it is fixed with pedestal along the distal end of the rotating disk cantilever of the radially uniform distribution of rotating shaft and fixes support, and support is fixed in part base Ultra-clear glasses are provided with above position and raise worm cover, is respectively equipped with the front side of ultra-clear glasses feeding worm cover and rear side to seedling passage and receives seedling Passage, the pedestal of remainder is fixed support and can be located at enable the glide base through entering to seedling passage to the tail end of seedling passage Pedestal is enough fallen within to fix support or the glide base after ultra-clear glasses raise worm cover can be caused positioned at the initiating terminal for receiving seedling passage It can fall into and receive in seedling passage.
Described ultra-clear glasses raise worm cover in feeding worm cover geometrical clamp and raise worm cover geometrical clamp and fixed by raising worm cover Jig arm is fixed in channel bracket;Raise to be provided with worm cover in ultra-clear glasses and change seedling passage with banister brush so that insect can not lead to Cross change seedling passage into ultra-clear glasses raise worm cover in.
The rear side of described receipts seedling passage, which is provided with, receives seedling extension passage, to seedling passage, receipts seedling passage and receives seedling extension passage It is fixedly installed in channel bracket;The tail end to seedling passage is provided with the initiating terminal that seedling passage is switched and received to seedling provided with receipts seedling Switch, seedling switch is switched and received to seedling and is controlled respectively by corresponding mechanical switch assemblies.
The inner hollow of the glide base is to load seedling matrix, and glide base is provided with feeding worm and fixes branch with seedling Frame, raise worm and glide base is fixed on by adjustable retainer ring with seedling fixed support, glide base can be fixed by pedestal Switch is fixed on pedestal and fixed in support.
Described envirment factor control includes full-spectrum LED light source, the device assembly that filters/be polarized with monitoring system, added automatically Wet device, sensing system, gas release hole, blower fan and gas passage, described full-spectrum LED light source be correspondingly arranged at optical filtering/ It is polarized on the outside of device assembly, the corresponding position of electromagnetic shielding chamber corresponding to multigroup full-spectrum LED light source and the device assembly that filters/be polarized Shell for remaining position and full-spectrum LED light source of ultra-clear glasses and electromagnetic shielding chamber is that aluminum material is complete to be complementarily shaped to Aluminium shell, so as to play faraday cup effect;Longitudinal section is in the automatic humidifier of isosceles right triangle around electromagnetic screen The surface for covering the top one week of chamber and automatic humidifier has humidification micropore;Described sensing system is located at face in rotating shaft and surpassed White glass is raised at the height and position of worm cover and twisted rotary shaft is set;Described gas passage positioned at electromagnetic shielding chamber lower section and with The lower hollow hole of rotating shaft is connected, and the bottom of rotating shaft is evenly equipped with gas release hole, and blower fan is provided with the bottom of electromagnetic shielding chamber, Fan operation enables the gas for entering electromagnetic shielding chamber through gas passage, gas release hole fully to mix.
Described sensing system includes light intensity meter, Temperature Humidity Sensor, fluxgate meter and specific gas concentration meter, sensing Device system is connected by data wire with power supply and control centre to be recorded data in real time and feed back to power supply and control centre.
Described power supply and control centre outside electromagnetic shielding chamber, and envirment factor control and monitoring system and power supply and Control centre is connected.
Described test worm activities monitoring system includes being arranged on the infrared varifocal camera on rotating shaft top and installed in electricity Behavioural analysis software in source and control centre, infrared varifocal camera are connected by data wire with power supply and control centre Connect.
Described rotating shaft by bearing be arranged on electromagnetic shielding chamber top cover on and rotating shaft top set stepper motor and Actuator assembly comes the rotation of drive shaft, stepper motor and actuator assembly and is connected by circuit with power supply and control centre Connect, rotating disk spiral arm is fixed on rotating shaft by rotation shaft regulated ring;Described stepper motor and actuator assembly, rotating shaft, bearing, rotating disk Spiral arm, rotation shaft regulated ring, glide base fix support and power supply and control centre forms all the period of time core rotating table system.
The top and the bottom of the electromagnetic shielding chamber set a pair of outer cover locks respectively, and outer cover lock can open respectively and Motionless electromagnetic Seedling and test worm are placed or collected in shielding cavity top and side to facilitate.
The present invention has the following advantages compared with prior art:
Apparatus of the present invention structural integrity, multiple functional and simple to operate, the data of acquisition are accurate, package unit small volume, increase income Property it is strong, can be used with any extraneous artificial magnetic field collocation, can also be moved to the place of needs at any time, each component is in device Nonferromugnetic material, the interference of experiment accuracy may be handled carrying out artificial magnetic field by having taken into account ferromagnetic material;The electricity of aluminum Magnetic screen chamber can effectively shield extraneous medium-high frequency interference electromagnetic field and electric field, improve the repeatability of magnetic field processing experiment;Ring Border factor control can need to provide specific light source characteristic with monitoring system according to experiment(The adjustable photoperiod, light intensity, frequency, wavelength, Polarized light source), humiture, magnetic field intensity and gaseous species and concentration, and individual features parameter can be recorded in real time, there is provided high-precision Accurate envirment factor processing scheme;Test worm activities monitoring system can round-the-clock record insect lipids activity, and can by power supply and The behavioural analysis software that control centre integrates carries out on-line analysis, efficient quick;All the period of time core rotating table system combines to be received to seedling Seedling system can as requested all the period of time collect detected sample automatically and ground with carrying out follow-up physiology and the Molecular Biology Mechanism Study carefully, effectively reduce manpower, reduce human error, improve conventional efficient and result accuracy, thus the device it is reasonable in design, open Source is efficient, time saving and energy saving, accurate easily-controllable, suitably promotes the use of.
Brief description of the drawings
Accompanying drawing 1 monitors the vertical section structural representation for receiving sampling device for the small insects physiological behavior rhythm and pace of moving things of the present invention automatically Figure;
Accompanying drawing 2 monitors the Structure of cross section schematic diagram for receiving sampling device for the small insects physiological behavior rhythm and pace of moving things of the present invention automatically;
The feeding worm device detailed structure schematic diagram of the present invention of accompanying drawing 3;
Accompanying drawing 4 is the present invention to seedling passage and the detailed structure schematic diagram for receiving seedling passage.
Wherein:1-stepper motor and actuator assembly;2-rotating shaft;3-full-spectrum LED light source;4-optical filtering/the polarizer Component;5-bearing;6-automatic humidifier;7-infrared varifocal camera;8-sensing system;9-feeding worm is consolidated with seedling Fixed rack;10-raise worm cover fixing clamp arm;11-raise worm cover geometrical clamp;12-ultra-clear glasses raise worm cover;13-rotating disk spiral arm; 14-give seedling passage;15-glide base;16-channel bracket;17-switched to seedling;18-receive seedling switch;19-receipts seedling leads to Road;20-rotation shaft regulated ring;21-outer cover is locked;22-receive seedling extension passage;23-gas release hole;24-blower fan;25-gas Body passage;26-electromagnetic shielding chamber;27-power supply and control centre;28-change seedling passage;29-banister brush;30-pedestal is fixed Switch;31-pedestal fixes support;32-adjustable retainer ring;33-mechanical switch assemblies.
Embodiment
The present invention is further illustrated with embodiment below in conjunction with the accompanying drawings.
As Figure 1-4:A kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device automatically, Including being electromagnetically shielded chamber 26, the top and the bottom of electromagnetic shielding chamber 26 set a pair of outer cover locks 21 respectively, and outer cover lock 21 can be beaten respectively Open and place or collect seedling and test worm with the top of Motionless electromagnetic shielding cavity 26 and side to facilitate.It is provided with electromagnetic shielding chamber 26 Envirment factor controls with monitoring system, test worm activities monitoring system, to seedling and receives seedling system and several portions of all the period of time core rotating table system Point, the rotating shaft 2 of all the period of time core rotating table system is vertically arranged in the middle part of electromagnetic shielding chamber 26, along radially uniform point of rotating shaft 2 The distal end of the rotating disk cantilever 13 of cloth is fixed with pedestal and fixes support 31, and is fixed in part base above the position of support 31 and be provided with ultrawhite Glass raises worm cover 12, and ultra-clear glasses raise worm cover 12 and are arranged in feeding worm cover geometrical clamp 11 and raise worm cover geometrical clamp 11 by raising worm cover Fixing clamp arm 10 is fixed in channel bracket 16, is raised to be provided with worm cover 12 in ultra-clear glasses and is changed seedling passage 28 with banister brush 29, So that insect can not be entered in ultra-clear glasses feeding worm cover 12 by changing seedling passage 28;The front side of worm cover 12 is raised with after in ultra-clear glasses Side is respectively equipped with to seedling passage 14 and receives seedling passage 19, and the rear side for receiving seedling passage 19 is provided with receipts seedling extension passage 22, gives seedling passage 14th, receive seedling passage 19 and receive seedling extension passage 22 and be fixedly installed in channel bracket 16, the tail end to seedling passage 14 is provided with to seedling The initiating terminal of switch 17 and receipts seedling passage 19 is provided with receipts seedling switch 18, and 18 are switched respectively by corresponding machine with seedling is received to seedling switch 17 Tool switch module 33 controls, and the pedestal of remainder, which fixes support 31, to be caused positioned at the tail end to seedling passage 14 through giving seedling passage 14 glide bases 15 entered can fall within pedestal and fix support 31 or can cause positioned at the initiating terminal for receiving seedling passage 19 by super Glide base 15 after white glass feeding worm cover 12, which can be fallen into, to be received in seedling passage 19.The inner hollow of glide base 15 is to load children Seedling matrix, and glide base 15 is provided with and raises worm seedling fixed support 9, feeding worm seedling fixed support 9 passes through adjustable solid Determine ring 32 and be fixed on glide base 15, glide base 15 can be fixed switch 30 by pedestal and is fixed in pedestal fixation support 31.
In said apparatus, envirment factor control with monitoring system include full-spectrum LED light source 3, filter/be polarized device assembly 4th, automatic humidifier 6, sensing system 8, gas release hole 23, blower fan 24 and gas passage 25, the correspondence of full-spectrum LED light source 3 The outside of device assembly 4 of filtering/be polarized is arranged on, is electromagnetically shielded corresponding to multigroup full-spectrum LED light source 3 and the device assembly 4 that filters/be polarized It is aluminium that the corresponding position of chamber 26, which is ultra-clear glasses and is electromagnetically shielded remaining position of chamber 26 and the shell of full-spectrum LED light source 3, Saw lumber matter is to be complementarily shaped to complete aluminium shell, so as to play faraday cup effect;Longitudinal section is in isosceles right triangle Automatic humidifier 6 has around the surface on the top one week of electromagnetic shielding chamber 26 and automatic humidifier 6 humidifies micropore;Sensor system System 8 is raised positioned at face ultra-clear glasses in rotating shaft 2 at the height and position of worm cover 12 and twisted rotary shaft 2 is set, and sensing system 8 includes Light intensity meter, Temperature Humidity Sensor, fluxgate meter and specific gas concentration meter, sensing system 8 pass through data wire and power supply and control Center 27 processed is connected to be recorded data in real time and feed back to power supply and control centre 27, and power supply and control centre 27 are positioned at electricity Magnetic screen chamber 26 is outer to avoid producing interference of electromagnetic field experiment, and envirment factor controls and monitoring system and power supply and control centre 27 are connected;Gas passage 25 is connected positioned at the lower section of electromagnetic shielding chamber 26 and with the lower hollow hole of rotating shaft 2, rotating shaft 2 Bottom is evenly equipped with gas release hole 23, and blower fan 24 is provided with the bottom of electromagnetic shielding chamber 26, and blower fan 24, which is run, to lead to through gas The gas that road 25, gas release hole 23 enter electromagnetic shielding chamber 26 can be mixed fully.Test worm activities monitoring system includes setting In the infrared varifocal camera 7 on the top of rotating shaft 2 and the behavioural analysis software in power supply and control centre 27, it is infrared can Zoom camera 7 is connected by data wire with power supply and control centre 27.Rotating shaft 2 is arranged on electromagnetic shielding chamber by bearing 5 On the 26 top cover and top of rotating shaft 2 sets stepper motor and actuator assembly 1 to carry out the rotation of drive shaft 2, stepper motor and Actuator assembly 1 is connected by circuit with power supply and control centre 27, and rotating disk spiral arm 13 is fixed on by rotation shaft regulated ring 20 Rotating shaft 2;Described stepper motor and actuator assembly 1, rotating shaft 2, bearing 5, rotating disk spiral arm 13, rotation shaft regulated ring 20, slip base The fixed support 31 of seat and power supply and control centre 27 form all the period of time core rotating table system.
In said apparatus, it is non-in electromagnetic shielding chamber 26 or to produce each component mechanically connected with electromagnetic shielding chamber 26 Ferromagnetic fiberglass, lucite, resin or aluminum products, power generator and motor etc. mainly produce magnetic device in electromagnetism Outside shielding cavity 26, influence when carrying out experiment in magnetic field to reduce to artificial magnetic field.
Technical scheme is further illustrated below by specific embodiment.
A kind of insect physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device, including envirment factor control automatically Seedling system, all the period of time core rotating table system and electromagnetic shielding framework five are received with monitoring system, test worm activities monitoring system, to seedling Point.
In envirment factor control and monitoring system, full-spectrum LED light source 3 is respectively positioned on three device assemblies 4 that filter/be polarized Outside, to control the wavelength of light source and polarization characteristic, three groups of full-spectrum LED light sources 3 and the device assembly 4 that filters/be polarized are located at respectively Be electromagnetically shielded the top of chamber 26 and both sides, electromagnetic shielding chamber 26 relevant position be ultra-clear glasses to provide good light transmission, electricity Remaining position of magnetic screen chamber 26 and the shell of full-spectrum LED light source 3 be aluminum material to be complementarily shaped to complete aluminium shell, from And play faraday cup effect;Frequency, photoperiod and the light intensity of full-spectrum LED light source 3 are regulated and controled by power supply and control centre 27, The device assembly 4 that filters/be polarized needs concurrently or separately to configure to obtain specific wavelength/polarized light source according to experiment.Longitudinal section in etc. The automatic humidifier 6 of waist right angled triangle has around the surface on the top of electromagnetic shielding chamber 26 one week and automatic humidifier 6 to be added Wet micropore;Sensing system 8 includes light intensity meter, Temperature Humidity Sensor, fluxgate meter and specific gas concentration meter, sensing system 8 face ultra-clear glasses are raised at the height and position of worm cover 12 in the rotating shaft 2 and twisted rotary shaft 2 is set, and sensing system 8 passes through number It is connected according to line with power supply and control centre 27 to be recorded data in real time and feed back to power supply and control centre 27;Gas passage 25 are located at the lower section of electromagnetic shielding chamber 26 and are connected with the lower hollow hole of rotating shaft 2, and the bottom of rotating shaft 2 is evenly equipped with gas release Hole 23, blower fan 24 is provided with the bottom of electromagnetic shielding chamber 26, blower fan 24, which is run, to enter through gas passage 25, gas release hole 23 Entering to be electromagnetically shielded the gas of chamber 26 can fully mix, it is ensured that gas concentration controllable precise.
In test worm activities monitoring system, as shown in Figure 1:Infrared varifocal camera 7 is located at the feeding worm device shown in Fig. 3 Oblique upper, corresponding six groups of ultra-clear glasses feeding worm cover 12, focuses to optimum field before testing, can round the clock monitor and record test worm behavior work It is dynamic, and carry out statistics plot analysis by being integrated in power supply and storing the behavioural analysis software of control centre 27.
Received to seedling in seedling system, to totally ten two, seedling passage 14, receive totally six, seedling passage 19, except corresponding six groups of ultra-clear glasses Raise without to seedling passage 14 and receipts seedling passage 19 at worm cover 12, every group of ultra-clear glasses raise worm cover 12, and upside continuously has two clockwise Individual to give seedling passage 14, downside is provided with one and receives seedling passage 19 clockwise;To seedling passage 14, receive seedling passage 19 and receipts seedling extension Passage 22 supports series connection by channel bracket 16, can be contacted just with pedestal fixation support 31 to seedling passage 14 with receiving seedling passage 19 It is connected;The hollow inside of glide base 15 can load seedling matrix(Nutrient solution or solid fertilizer), raise worm seedling fixed support 9 Specification can be slightly changed according to seedling stem and is fixed on adjustable retainer ring 32 on glide base 15;To seedling receive seedling system it is complete from Dynamicization is realized, mainly by being realized to seedling switch 17, receipts seedling switch 18, pedestal fixation switch 30 and mechanical switch assemblies 33, base The fixed support 31 of seat drives rotation to one to seedling passage 14 by rotating disk spiral arm 13, collides and switchs 17 to seedling, glide base 15 together with Examination seedling thereon can slide to pedestal and fix in support 31, and fixes switch 30 by pedestal and fix, by not allowing insect escape Change seedling passage 28 and banister brush 29 turns to ultra-clear glasses and raised in worm cover 12 so that test worm freely activity, feeding, examination on new talent Worm after the feeding time of setting, with the feeding worm that moving base is fixed in support 31 and glide base 15 with seedling consolidated by rotating disk spiral arm 13 Fixed rack 9 again turn clockwise to bottom receipts seedling passage 19, and hit receive seedling switch 18, cooperative mechanical switch module 33 is complete The seedling treated into test worm is collected, and is passed to receipts seedling passage 19 and is slipped into receipts seedling extension passage 22, in case carrying out follow-up examination Worm egg laying amount, the feeding rhythm and pace of moving things and seedling by when feeding response etc. physiology or Study on Molecular Mechanism.
All the period of time core rotating table system is operating clockwise, and power resources are stepper motor and actuator assembly 1, according to electricity Source and the setting for storing control centre 27, link and rotated by the rotating shaft 2 that bearing 5 is fixed by specified time interval, rotating disk spiral arm 13 Band moving base fix support 31, integrate to seedling receive seedling system be automatically finished to seedling, receive seedling, change seedling work.Rotation shaft regulated 20, ring The level height of the customization altitude calibration rotating disk of support 31 is fixed according to pedestal, is aided in providing more accurately experiment.
Being electromagnetically shielded framework includes electromagnetic shielding chamber 26 and the aluminium shell part of full-spectrum LED light source 3 of aluminum, outer cover Lock 21 can open top half as needed, by opening rotation shaft regulated ring 20, be removed together in conjunction with rotating disk, to be tieed up Shield, while conveniently the glide base 15 with examination seedling is put into seedling passage 14, and be advantageous to place test worm in ultra-clear glasses feeding Worm cover 12;Outer cover lock 21 can open lateral parts as needed, to be collected after the experiment of the whole rhythm and pace of moving things terminates positioned at receipts seedling extension Seedling after the test worm processing of passage 22, for carrying out subsequent physiological, molecular biology experiment.
Apparatus of the present invention structural integrity, multiple functional and simple to operate, the data of acquisition are accurate, package unit small volume, Increasing income property is strong, can be used with any extraneous artificial magnetic field collocation, can also be moved to the place of needs at any time, each component in device It is nonferromugnetic material, the interference of experiment accuracy may be handled carrying out artificial magnetic field by having taken into account ferromagnetic material;Aluminum Electromagnetic shielding chamber 26 can effectively shield extraneous medium-high frequency interference electromagnetic field and electric field, improve the repeatable of magnetic field processing experiment Property;Envirment factor controls can need to provide specific light source characteristic with monitoring system according to experiment(The adjustable photoperiod, light intensity, frequency, Wavelength, polarized light source), humiture, magnetic field intensity and gaseous species and concentration, and individual features parameter can be recorded in real time, there is provided High accurately envirment factor processing scheme;Test worm activities monitoring system round-the-clock can record insect lipids activity, and can pass through The behavioural analysis software that power supply and control centre 27 integrate carries out on-line analysis, efficient quick;All the period of time core rotating table system knot Close to seedling receive seedling system can as requested all the period of time collect detected sample automatically to carry out follow-up physiology and molecular biosciences Mechanism Study is learned, effectively reduces manpower, reduces human error, improve conventional efficient and result accuracy, therefore the device is set Meter is reasonable, it is efficient, time saving and energy saving to increase income, accurate easily-controllable, suitably promotes the use of.
The technological thought of above example only to illustrate the invention, it is impossible to protection scope of the present invention is limited with this, it is every According to technological thought proposed by the present invention, any change done on the basis of technical scheme, the scope of the present invention is each fallen within Within;The technology that the present invention is not directed to can be realized by prior art.

Claims (10)

1. a kind of small insects physiological behavior rhythm and pace of moving things available for magnetic field processing monitors receipts sampling device, including electromagnetic shielding chamber automatically (26), it is characterised in that:Described electromagnetic shielding chamber(26)It is provided with envirment factor control and monitoring system, test worm movement monitoring System, to seedling receive seedling system and all the period of time core rotating table system, the rotating shaft of all the period of time core rotating table system(2)It is vertically arranged in electricity Magnetic screen chamber(26)Middle part, along rotating shaft(2)Radially uniform distribution rotating disk cantilever(13)Distal end be fixed with pedestal and fix Support(31), and fix and hold in the palm in part base(31)Position above be provided with ultra-clear glasses raise worm cover(12), worm is raised in ultra-clear glasses Cover(12)Front side and rear side be respectively equipped with and give seedling passage(14)With receipts seedling passage(19), the pedestal of remainder, which is fixed, to be held in the palm(31) It can be located at and give seedling passage(14)Tail end cause through giving seedling passage(14)The glide base of entrance(15)Pedestal can be fallen within to consolidate Fixed support(31)Or it can be located at and receive seedling passage(19)Initiating terminal cause by ultra-clear glasses raise worm cover(12)Glide base afterwards (15)It can fall into and receive seedling passage(19)It is interior.
2. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described ultra-clear glasses raise worm cover(12)Installed in feeding worm cover geometrical clamp(11)In and raise worm cover geometrical clamp (11)By raising worm cover fixing clamp arm(10)It is fixed on channel bracket(16)On;Worm cover is raised in ultra-clear glasses(12)It is provided with soft Hairbrush(29)Change seedling passage(28)So that insect can not be by changing seedling passage(28)Worm cover is raised into ultra-clear glasses(12)It is interior.
3. the small insects physiological behavior rhythm and pace of moving things according to claim 1 or 2 available for magnetic field processing monitors receipts sample automatically Device, it is characterised in that:Described receipts seedling passage(19)Rear side be provided with receive seedling extension passage(22), give seedling passage(14), receive Seedling passage(19)Extend passage with seedling is received(22)It is fixedly installed on channel bracket(16)On;It is described to give seedling passage(14)Tail end set Have and switched to seedling(17)And receive seedling passage(19)Initiating terminal be provided with receive seedling switch(18), switched to seedling(17)Switched with seedling is received (18)Respectively by corresponding mechanical switch assemblies(33)Control.
4. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:The glide base(15)Inner hollow to load seedling matrix, and glide base(15)It is provided with and raises Worm seedling fixed support(9), raise worm seedling fixed support(9)Pass through adjustable retainer ring(32)It is fixed on glide base (15), glide base(15)It can be fixed and switched by pedestal(30)It is fixed on pedestal and fixes support(31)On.
5. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described envirment factor control includes full-spectrum LED light source with monitoring system(3), optical filtering/polarizer group Part(4), automatic humidifier(6), sensing system(8), gas release hole(23), blower fan(24)And gas passage(25), it is described Full-spectrum LED light source(3)Be correspondingly arranged at filter/be polarized device assembly(4)Outside, multigroup full-spectrum LED light source(3)And filter Light/be polarized device assembly(4)Corresponding electromagnetic shielding chamber(26)Corresponding position for ultra-clear glasses and electromagnetic shielding chamber(26)'s Remaining position and full-spectrum LED light source(3)Shell for aluminum material to be complementarily shaped to complete aluminium shell, so as to play method Cage is drawn to act on;Longitudinal section is in the automatic humidifier of isosceles right triangle(6)Around electromagnetic shielding chamber(26)Top one week And automatic humidifier(6)Surface have humidification micropore;Described sensing system(8)Positioned at rotating shaft(2)Upper face ultrawhite glass Glass raises worm cover(12)Height and position at and twisted rotary shaft(2)Set;Described gas passage(25)Positioned at electromagnetic shielding chamber (26)Lower section and and rotating shaft(2)Lower hollow hole be connected, rotating shaft(2)Bottom be evenly equipped with gas release hole(23), It is electromagnetically shielded chamber(26)Bottom be provided with blower fan(24), blower fan(24)Operation causes through gas passage(25), gas release hole (23)Into electromagnetic shielding chamber(26)Gas can fully mix.
6. the small insects physiological behavior rhythm and pace of moving things according to claim 5 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described sensing system(8)It is dense comprising light intensity meter, Temperature Humidity Sensor, fluxgate meter and specific gas Degree meter, sensing system(8)Pass through data wire and power supply and control centre(27)It is connected so that data are recorded and fed back in real time To power supply and control centre(27).
7. the small insects physiological behavior rhythm and pace of moving things according to claim 6 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described power supply and control centre(27)Positioned at electromagnetic shielding chamber(26)Outside, and envirment factor control is with supervising Examining system and power supply and control centre(27)It is connected.
8. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described test worm activities monitoring system includes being arranged on rotating shaft(2)The infrared varifocal camera on top (7)With installed in power supply and control centre(27)In behavioural analysis software, infrared varifocal camera(7)By data wire and Power supply and control centre(27)It is connected.
9. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:Described rotating shaft(2)Pass through bearing(5)Installed in electromagnetic shielding chamber(26)Top cover on and rotating shaft(2)'s Top sets stepper motor and actuator assembly(1)Carry out drive shaft(2)Rotation, stepper motor and actuator assembly(1)It is logical Cross circuit and power supply and control centre(27)It is connected, rotating disk spiral arm(13)Pass through rotation shaft regulated ring(20)It is fixed on rotating shaft(2); Described stepper motor and actuator assembly(1), rotating shaft(2), bearing(5), rotating disk spiral arm(13), rotation shaft regulated ring(20), it is sliding Moving base fixes support(31)With power supply and control centre(27)Form all the period of time core rotating table system.
10. the small insects physiological behavior rhythm and pace of moving things according to claim 1 available for magnetic field processing monitors automatically receives sample dress Put, it is characterised in that:The electromagnetic shielding chamber(26)Top and the bottom a pair of outer covers lock is set respectively(21), outer cover lock(21)Can Open and Motionless electromagnetic shielding cavity respectively(26)Seedling and test worm are placed or collected in top and side to facilitate.
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