CN107252088A - A kind of preparation method of food for Abnormal Lipid Metabolism type obesity - Google Patents
A kind of preparation method of food for Abnormal Lipid Metabolism type obesity Download PDFInfo
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- CN107252088A CN107252088A CN201610738472.6A CN201610738472A CN107252088A CN 107252088 A CN107252088 A CN 107252088A CN 201610738472 A CN201610738472 A CN 201610738472A CN 107252088 A CN107252088 A CN 107252088A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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Abstract
A kind of preparation method of food for Abnormal Lipid Metabolism type obesity, its step includes：(1) genetic test is carried out to 4 related gene locis of Abnormal Lipid Metabolism；(2) according to genetic test result, food formula is determined；(3) according to food formula, food is made.This method gene loci with gene unrighted acid interaction main by detecting and integrating, by classification analysis, find target group, develop the food making method with conditioning obesity action, eliminate the blindness and inefficiencies of people's fat-reducing, help people to cut down expenses and can quickly reduce weight, so as to prevent a series of generation of obesity-related diseases, allow everybody easily to enjoy healthy living.
Description
Technical field
The invention belongs to Nutrigenomics field, it is related to a kind of system of the food for Abnormal Lipid Metabolism type obesity
A kind of Preparation Method, and in particular to the preparation of the conditioning food of obesity crowd according to gene-nutrition interaction principle design
Method.
Background technology
Obesity is the positive energy balance (calorie intake as caused by h and E factor>Energy expenditure) caused knot
Really.The variation of body weight about 70% is attributed to heredity in crowd.Some people, which drink water, to get fat, and this is relevant with gene.Heredity
Factor determines set point, intermediate supersession and the physical exertion of appetite.Protein coded by obesity-related gene is a kind of appetite
With energy balance adjust approach part, and this approach it is unbalance directly or indirectly cause fat accumulation and body weight
Increase.
According to World Health Organization 2015：2014, the whole world 18 years old and the above adult in have 39% (1,900,000,000 people's)
People is overweight, wherein there is the people of 13% (600,000,000 people) fat.It is overweight relative to 2010,18 years old 2014 and in the adult of the above
Person adds 2.4%, and overweight people adds 1.4%.2014, China 18 years old and the above adult in have 34.4% people surpass
Weight, wherein there is 6.9% people fat.Obesity can cause a series of diseases, specifically be shown in Table 1.
The health risk table relevant with obesity of table 1
When exploring the human diseases cause of disease, it can often trigger the debate of congenital reason and the reason day after tomorrow.Nowadays, biologists
Think, the either congenital or reason day after tomorrow finally controls the molecular process of human health without method interpretation.In most cases, it is special
Determine the presence of gene or mutation, only show there is neurological susceptibility to specified disease.But whether the hereditary potential is eventually exhibited as certain
Disease is planted, is interacted depending on the complexity between human genome and environment and behavial factor.This theory promote it is many with
The multi-disciplinary method of health and disease is studied based on gene.Nutrigenomics are the methods of one of them.It is by gene
Group science and nutrition, other lifestyle variables are such as smoked and combined with drinking.Although gene pairs function determines heavy to closing
Will, but nutrition plays modification to the expression degree of various genes, so as to whether obtain the determination of its genetic background to individual
Potentiality are adjusted.Nutrigenomics initially refer to research of the nutrient on the expression influence of individual inheritance structure.In recent years
Come, this definition has expanded to cover the trophic factor that Protecting gene group escapes injury.Generally speaking, Nutrigenomics are related to drink
Eat influence of the composition to genome, protein group (summations of all albumen) and metabolism (summations of all metabolins).Such as medicine
In thing genomics, influence of the medicine to different crowd is different the same, and scientific research personnel thinks that only part population is to specific nutrition
Intervene reaction positive, and remaining people is then slow in reacting, some people are even it is also possible that adverse reaction.
Scholarly forecast, Nutrigenomics would is that the new focus that the relief after pharmacogenomics catches people's attention
Section.It not only facilitates people and more fully understands that individual is produced by gene difference to various food ingredients and diet style
Raw differential responses, and related vegetative gene group data also can be that the effective dietotherapy scheme of specific crowd development be laid in a down-to-earth manner
Basis.The future development of Nutrigenomics is expected to as pharmacogenomics makes " personalised drug ", is people's amount
Body is customized to be gone out to meet " the personalized food " of individual demand.
The preparation method of current obesity Related product mainly has 2 classes, prepared by the food that a class applies to all groups
Method, sees Zheng Yanming patent《Fig Chinese date extract of pre- preventing obesity and preparation method thereof》, but exist carbohydrate,
Fat and protein intake control the non-individuals mode of clean cut；Another kind of is the food preparation side based on gene
Method, sees fragrant et al. patent of obliging《The method that individualized nutrition compound is manufactured according to gene pleiomorphism》, its content
It is that there is provided personalized method of weight-reducing, such as supplement bigarabe flavonoids, Roselle extract according to the gene pleiomorphisms of multiple genes
Or its combination, banana skin extract, vitamin B6, vitamin B12 or its combination, lotus leaf extract, white kidney beans extract, vegetables and fruits
Ferment, camellia extract or its combination, climing more declining extract, Green tea extract or its combination, the method is effective, but raw material is difficult
Obtain, and trivial operations, thus be unfavorable for promoting.Due to about 70% weight differences be as caused by inherent cause,
The different people of genetic constitution should take different fat-reducing strategies when suffering from obesity.Based on fat-reducing effect and notable allele
Number is relevant, and crowd's high-efficient fat reducing of excessive risk genetic constitution can effectively be distinguished and helped to the foundation of 3 grades of models.Therefore, I
Using newest achievement in research both at home and abroad, a food making method based on gene-nutrition interaction is developed, in daily life
In can lose weight, realize fat-reducing personalization and convenience, with larger social effect and market prospects.
The content of the invention
It is an object of the invention to overcome the shortcomings of that prior art is present, it is main by detecting and integrating have gene-
The gene loci of unrighted acid interaction, by classification analysis, finds target group, develops with conditioning obesity action
Food making method, eliminates the blindness and inefficiencies of people's fat-reducing, helps people to cut down expenses and can quickly reduce weight, so that
Prevent a series of generation of obesity-related diseases, allow everybody easily to enjoy healthy living.
The present invention is achieved by the following technical solutions：
A kind of preparation method of food for Abnormal Lipid Metabolism type obesity, it comprises the following steps：
(1) pair four gene locis related to Abnormal Lipid Metabolism carry out genetic test；
(2) food formula is determined according to genetic test result；
(3) according to food formula, food is made.
According to the present invention, 4 gene locis related to Abnormal Lipid Metabolism carry out genetic test and referred to：Take
Sequencing technologies or chip technology carry out polymorphic detection (SNP detections) to related 4 gene locis.
According to the present invention, 4 described related genes are the genes for referring to significantly be regulated and controled by unrighted acid, point
It is not FTO (rs9939609), PPARG (rs1801282), ADIPOQ (rs2241766), PLIN4 (rs8102428).It is shown in Table 2：
2 unrighted acids of table-interaction of genes table
As shown in table 2, the gene related to unrighted acid has four.
It is described by genetic test result according to the present invention, determine that food formula refers to：It is every in four above-mentioned genes
Individual SNP site has and only a pair of alleles, and wherein 1 allele in this pair of allele is adjusted by unrighted acid
Control significantly (is referred to as notable site), by calculating notable allele quantity and grading, determines food formula.Rating result is shown in Table
3：
The notable allele quantity of table 3 and unrighted acid intervention effect grading table
| Grading | Notable allele quantity |
| Typically | [0,1] |
| Well (2 bottle/day) | [2,4] |
| Outstanding (1 bottle/day) | [5,8] |
As shown in table 3, analyzed by calculating, the gene loci related to unrighted acid there are 4, totally 8 etc.
Position gene, when the number of notable allele is 0-1, obesity is difficult in heredity, therefore without intervention；When notable equipotential base
When factor amount is 2-4, unrighted acid intervention has certain effect, and intervention effect grading is good；When notable number of alleles
Measure for 5-8 when, unrighted acid intervene it is very effective, intervention effect grading it is outstanding.Entered by the gene Clustering to crowd
Row is assessed, and is more beneficial for instructing personalized diet.
According to the present invention, as shown in figure 1, described according to formula, making food refers to：It is excellent for grading in step (2)
Elegant and good individual, makes formula, is formulated as follows：Peanut containing 10g, 15g melon seeds, 8g pine nuts, 2g lemons in per 500ml drinks
Lemon.Concrete operations flow is as follows：10g peanuts, 15g melon seeds, 8g pine nuts are weighed, shells, adds pulverizer, be crushed to pulverizer
30 mesh, are poured in 1L plastic bottle (food-grade), warm water to 500ml, firmly rock 10 times, plus 2g lemon juice seasonings, do
Into nut spread.The outstanding individual of grading eats 500ml nut spreads (1 bottle) daily, and the good individual of grading eats 1000ml (2 bottles) daily
Nut spread.
The present invention has following technique effect：
1st, the present invention is based on Nutrigenomics principle, than existing clean cut diet products and simple risk assessment
Product has the lose weight scheme of more personalized and operability, with great theory value and application value；
2nd, the food for preparing of the present invention use commercially available nut for raw material, preparation process simply, plus lemon seasoning, be one kind very
Product popular and with good fat-reducing effect.
Brief description of the drawings
Fig. 1 is a kind of workflow of the preparation method of food for Abnormal Lipid Metabolism type obesity of the present invention
Figure.
Fig. 2 is the grading of PPARG genes and the arachidonic graph of a relation of unrighted acid.Fig. 2 ordinate represents ratio
Value is than (odds ratio), and abscissa represents that arachidonic acid takes in (Tertile of arachidonic acid
intake)。
Embodiment
Embodiment 1
Pass through 4 gene loci FTO (rs9939609), PPARGs of the quantitative fluorescent PCR to Lee's (some individual)
(rs1801282), ADIPOQ (rs2241766), PLIN4 (rs8102428) are sequenced, and sequencing result is shown in Table 4：
The gene sequencing result table of Lee of table 4
| Gene | Site | Genotype |
| FTO | rs9939609 | TA |
| PPARG | rs1801282 | GG |
| ADIPOQ | rs2241766 | TT |
| PLIN4 | rs8102428 | AG |
As shown in Table 4, Lee has 6 notable allele (A, G, G, T, T, G), in the good class of grading.
Embodiment 2
10g peanuts, 15g melon seeds, 8g pine nuts are weighed, shells, adds pulverizer, 30 mesh are crushed to pulverizer, 1L is poured on
Plastic bottle (food-grade) in, warm water to 500ml is firmly rocked 10 times, plus 2g lemon juice seasonings, makes nut spread.Lee
Certain daily noon and evening respectively eat 1 bottle of nut spread, then have a meal, Body weight loss 5.2kg after 1 month.
Embodiment 3
15g peanuts, 10g melon seeds, 8g pine nuts are weighed, shells, adds pulverizer, 30 mesh are crushed to pulverizer, 1L is poured on
Plastic bottle (food-grade) in, warm water to 500ml is firmly rocked 10 times, plus 2g lemon juice seasonings, makes nut spread.Lee
Certain daily noon and evening respectively eat 1 bottle of nut spread, then have a meal, Body weight loss 4.3kg after 1 month.
Embodiment 4
10g peanuts, 10g melon seeds, 10g pine nuts are weighed, shells, adds pulverizer, 30 mesh are crushed to pulverizer, 1L is poured on
Plastic bottle (food-grade) in, warm water to 500ml is firmly rocked 10 times, plus 2g lemon juice seasonings, makes nut spread.Lee
Certain daily noon and evening respectively eat 1 bottle of nut spread, then have a meal, Body weight loss 4.1kg after 1 month.
Preferred embodiment of the invention described in detail above.Therefore, all technical staff in the art are according to this
The design of invention passes through the available technical side of logical analysis, reasoning, or a limited experiment on the basis of existing technology
Case, all should be in the protection domain being defined in the patent claims.
Claims (7)
1. a kind of preparation method of food for Abnormal Lipid Metabolism type obesity, it is characterised in that：Comprise the following steps：
(1) pair four gene locis related to Abnormal Lipid Metabolism carry out genetic test；(2) food is determined according to genetic test result
Formula；(3) according to the food formula, food is made.
2. the preparation method of the food according to claim 1 for Abnormal Lipid Metabolism type obesity, it is characterised in that：
Described genetic test is：Sequencing technologies or chip technology is taken to carry out polymorphic detection to four gene locis.
3. the preparation method of the food according to claim 1 or 2 for Abnormal Lipid Metabolism type obesity, its feature exists
In：Four described gene locis are the gene that can be significantly regulated and controled by unrighted acid.
4. the preparation method of the food according to claim 3 for Abnormal Lipid Metabolism type obesity, it is characterised in that：
Four described gene locis are FTO (rs9939609), PPARG (rs1801282), ADIPOQ (rs2241766), PLIN4
(rs8102428)。
5. the preparation method of the food according to claim 3 for Abnormal Lipid Metabolism type obesity, it is characterised in that：
Step (2) includes：Each SNP site has and only a pair of alleles, wherein 1 allele is by unrighted acid
Regulation and control are notable, by calculating notable allele quantity and grading, determine food formula.
6. the preparation method of the food according to claim 5 for Abnormal Lipid Metabolism type obesity, it is characterised in that：
Step (2) includes：For the outstanding and good individual of grading in step (2), the food formula is determined, the food formula is such as
Under：Peanut containing 10g, 15g melon seeds, 8g pine nuts, 2g lemons in per 500ml drinks；
If the quantity of notable allele is [0,1], then it is rated general；
If the quantity of notable allele is [2,4], then it is rated good；
If the quantity of notable allele is [5,8], then it is rated outstanding.
7. the preparation method of the food according to claim 6 for Abnormal Lipid Metabolism type obesity, it is characterised in that：
Step (2) includes：10g peanuts, 15g melon seeds and 8g pine nuts are weighed, 30 mesh is crushed to after shelling with pulverizer, is placed in 1L plastics
In bottle, warm water is to 500ml, after rocking uniformly, plus 2g lemon juice seasonings, makes nut spread；
It is rated outstanding individual edible 500ml nut spreads daily；
It is rated good individual edible 1000ml nut spreads daily.
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Application publication date: 20171017 |