CN107162947A - It is a kind of to prepare the method for stabilizing raphanin - Google Patents
It is a kind of to prepare the method for stabilizing raphanin Download PDFInfo
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- CN107162947A CN107162947A CN201710491907.6A CN201710491907A CN107162947A CN 107162947 A CN107162947 A CN 107162947A CN 201710491907 A CN201710491907 A CN 201710491907A CN 107162947 A CN107162947 A CN 107162947A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C331/00—Derivatives of thiocyanic acid or of isothiocyanic acid
- C07C331/16—Isothiocyanates
- C07C331/18—Isothiocyanates having isothiocyanate groups bound to acyclic carbon atoms
- C07C331/22—Isothiocyanates having isothiocyanate groups bound to acyclic carbon atoms of an unsaturated carbon skeleton
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Abstract
It is a kind of to prepare the method for stabilizing raphanin, belong to Separation of Natural Products technical field of purification.The present invention, by preparing raphanin, prepares anhydrous ingredient powder using degreasing rouge radish seed powder as raw material, prepares raphanin dispersion, prepares and stabilizes the steps such as raphanin, prepares the stabilisation raphanin that long shelf-life was not degraded up to 12~14 months.The present invention has considered influence of the external conditions such as illumination, temperature, aerobic environment to raphanin, and take the measure of lucifuge, low temperature, negative pressure and deoxidation nitrogen charging, the unfavorable factor that raphanin is degraded can be made by eliminating as much as possible, so as to significantly enhance the stability of raphanin；Production equipment used is industrial equipment, and working condition is easily implemented, easy to operate, is adapted to large-scale production；Produced in production process without " three wastes ", be typical green production process, easy to utilize, the stabilisation raphanin prepared can be widely applied in the field such as Drug development and research and health care of food product.
Description
First, technical field
The invention belongs to Separation of Natural Products technical field of purification, and in particular to a kind of to prepare the side for stabilizing raphanin
Method.
2nd, background technology
Raphanin is also known as raphanin, raphanin etc., and molecular formula is C6H9NOS2, entitled (E) the 4- isothiocyanic acids base of system-
1- (methylsulfinyl) -1- butylene, it is syrupy shape yellow liquid, soluble in water, methanol, ethanol, chloroform, second at normal temperatures
Ether equal solvent.Correlative study shows raphanin in anti-oxidant, antiviral, hypotensive, anti-platelet aggregation, rush digestion, the prevention heart
There is strong biological activity in terms of vascular diseases, and have substantially to liver cancer, breast cancer, lung cancer, cancer of the esophagus, NIH mice
Blocking effect, be a kind of natural active matter with market potential and economic value.However, due to temperature, illumination, solvent
Etc. the influence of factor, raphanin is degraded rapidly in production, transport and storage, and high-purity is disclosed at present although causing
Raphanin extraction process, but not yet there is stabilized raphanin, this just directly limit it as cancer therapy drug and enters one
Walk development and utilization.Therefore, research and develop a kind of prepare and stabilize the method for raphanin so as to extend the holding time of raphanin, will be right
It provides technical foundation as the practical application of a kind new medicine.
The existing research on raphanin stability, the thesis for the doctorate that the Tian Guifang as disclosed in 2016 writes《Natural production
The mechanism of degradation and stability study of thing raphanin》, a kind of technology using spray drying of the paper improves raphanin
The technique of stability, the process of the technique is：First prepare maltodextrin, hydroxypropyl beta cyclodextrin, soybean protein isolate, ox blood
The aqueous solution of the membrane materials such as pure albumen, chitosan, then adds raphanin, is turned each aqueous solution using experiment type spray drier
It is changed into micro-capsule powder, the stability of raphanin is improved with this.The major defect of this method is：1. raphanin is added what is prepared
In the membrane material aqueous solution, and raphanin is extremely unstable in aqueous, starts degraded in several minutes；When being 2. spray-dried
EAT reaches 180 DEG C, and degradation rate is faster at this temperature for raphanin；3. compared to the radish for not carrying out micro-capsule embedding
Element, although the micro-capsule embedding raphanin prepared can effective starvation, but due to raphanin 1. and 2. when it is obvious
Degraded, therefore, the method is difficult to prepare high-purity raphanin product.And for example Application No. 201510081274.2, entitled " carry
The patent of invention of the method for high raphanin stability ", the patent describe and make an addition to raphanin in polyethylene glycol or vegetable oil
Method to improve raphanin stability.The weak point of this method is：1. polyethylene glycol and vegetable oil are not carried out at dehydration
Reason, and raphanin contact even if with micro water and can also degraded, and causes its stability to reduce；2. not specifying need to be by raphanin
It is kept in dark place, and raphanin can then change accelerated decomposition under light illumination, again result in the reduction of its stability；3. the patent is obtained
To result be：At 360 days, degradation rate during -80 DEG C to 120 DEG C of raphanin significantly reduced to the 20.3% of control group~
35.8%.I.e. by the store method of the patent disclosure, raphanin is still being degraded, even in pole as -80 DEG C
Still in degraded at a temperature of end, illustrate that it does not have enough stability still, and the catabolite of raphanin can be more to human body generation
Toxic side effect is planted, thus, the raphanin that the method is preserved may not apply to medicine or food service industry.
3rd, the content of the invention
The present invention be directed to a kind of the not enough there is provided method for preparing stabilisation raphanin of existing raphanin store method,
This method has simple to operate, mild condition, using conventional commercial equipment, the features such as raphanin stability is high.
The present invention principle be：Microwave vacuum low-temperature drying equipment is one kind that microwave energy technology is combined with vacuum technique
Novel microwave energy application apparatus, it has had both microwave and vacuum drying series of advantages, it is adaptable to which processing can not be in high temperature bar
The sample being dried under part, is conducive to evading the extraneous factor that raphanin can be made to degrade, therefore can enter raphanin with it
Row drying；Grinding distribution machine be it is a kind of under high speed conditions by the plant equipment of the dispersed mixing of material, can be entered using it
The dispersion mixing of row raphanin and anhydrous ingredient powder, so as to make raphanin dispersion；Freeze-drying is that water-containing materials are first
It is chilled to below freezing, water is changed into ice, the drying means that then ice is changed into steam and removed under high vacuum, Lay
Fu elements are extremely unstable in aqueous, easily degrade, therefore raphanin dispersion can be dehydrated using Freeze Drying Technique
Dry, to prepare the raphanin that water content is extremely low；Raphanin is the isothiocyanic acid base class compound containing a carbon-carbon double bond,
With extremely strong antioxidation activity, therefore need to carry out it processing of deoxidation nitrogen charging, and sealing preserve in preparation process, with
Strengthen the stability of the raphanin prepared；Raphanin is a kind of photoactive substance, is easily activated by light and occurs photochemistry drop
Solution, therefore lucifuge is needed during preparation and preservation, with the stability for the raphanin for strengthening preparation；Raphanin is a kind of temperature sensitive thing
Matter, the rise of temperature can accelerate the degraded of raphanin, therefore temperature is unsuitable too high during preparation and preservation, to strengthen preparation
Raphanin stability.
The purpose of the present invention is realized by following approach：It is a kind of to prepare the method for stabilizing raphanin, with degreasing rouge
Fat Radish seed powder is raw material, by preparing raphanin, prepares anhydrous ingredient powder, prepares raphanin dispersion, prepares and stabilizes radish
The steps such as element, prepare the stabilisation raphanin that long shelf-life was not degraded up to 12~14 months.Its specific processing step is such as
Under：
(1) raphanin is prepared
Using the existing technology for preparing raphanin, the method introduced according to Patent No. 201510270292.5, by super
The step such as filter and nanofiltration UF membrane, extraction, preparation chromatogram and high pressure low temperature concentration prepares the raphanin that purity is higher than 99%, is used for
(3) step prepares raphanin dispersion.
(2) anhydrous ingredient powder is prepared
The commercially available powdered fructose of food or pharmaceutical grade, glucose, mannose, mannitol, D-sorbite etc. are placed in micro-
In ripple low-temperature vacuum drying case, vacuum be 0.06~0.09MPa, temperature be 40~45 DEG C under conditions of be dried 1~3
Hour, that is, anhydrous powder shape fructose, glucose, mannose, mannitol, D-sorbite are prepared, also known as anhydrous ingredient powder is used for
Lower step prepares raphanin dispersion.
(3) raphanin dispersion is prepared
After the completion of first two steps, the anhydrous ingredient powder quality that the radish pixel volume prepared according to (1) step is prepared with (2) step
The ratio between (L/kg) be 1: 5~10 ratio, first will (2) step prepare anhydrous ingredient powder input grinding distribution machine in, grinding 5~
The raphanin for again preparing (1) step after 10 minutes is put into, and 15~30 points are ground under conditions of rotating speed is 6000~9000rpm
Clock, that is, prepare raphanin dispersion, is prepared for lower step and stabilizes raphanin.
(4) prepare and stabilize raphanin
After the completion of (3) step, the raphanin dispersion that (3) step is prepared is sub-packed in the brown that volume is 10~20mL
In vial, bottle stopper for lyophilized powder formulation is placed, then is placed in low temperature refrigerator, band gland is put into after 5~10h of precooling at -40~-20 DEG C
In the vacuum freeze drier of device, temperature be -50~-40 DEG C, vacuum be to be freeze-dried 24~48h under 20~50Pa,
Then nitrogen is passed through into freeze drier, is continued with 15~30 minutes, last gland sealing prepares stabilisation radish
Element.Temperature is placed on to be kept in dark place 12~14 months under conditions of -20~4 DEG C, raphanin purity remains at more than 99%.
The present invention is after adopting the above technical scheme, mainly have the following effects：
1st, the inventive method prepares raphanin of the purity higher than 99% using degreasing Radish seed powder as raw material, first, then passes through
Microwave low-temperature vacuum drying, grinding distribution, freeze-drying, deoxidation nitrogen charging etc. are operated, and are prepared the shelf-life and are 12~14 months
Without the stabilisation raphanin degraded, product quality meets market demands, and production equipment used is industrial equipment, raw
Production condition is easily implemented, easy to operate, is adapted to large-scale production.
2nd, the inventive method is dehydrated using microwave low-temperature vacuum drying technology to solid adjuvant material, is employed again cold
Freeze dry technology and dehydration and deoxidation nitrogen charging processing carried out to raphanin, be stripped of to greatest extent in product may remaining moisture and
Oxygen, significantly enhances the stability of raphanin.
3rd, the inventive method has also considered influence of the external conditions such as illumination, temperature, aerobic environment to raphanin, and
Take the measure of lucifuge, low temperature, negative pressure and deoxidation nitrogen charging, eliminate as much as possible raphanin can be made to degrade it is unfavorable because
Element, so as to significantly enhance the stability of raphanin.
4th, the formulation method that the present invention is provided includes addition fructose, glucose, mannose, mannitol, D-sorbite etc.
Solid pharmaceutical preparation, enriches the store method of raphanin, also causes the stabilisation raphanin prepared to can be widely applied to medicine and grind
Study carefully and develop and the field such as health care of food product in.
5th, produced in production process of the present invention without " three wastes ", be typical green production process, it is easy to utilize.
4th, embodiment
With reference to embodiment, the present invention is further illustrated.
Embodiment 1
A kind of to prepare the method for stabilizing raphanin, its concrete technology step is as follows：
(1) raphanin is prepared
Using the existing technology for preparing raphanin, the method introduced according to Patent No. 201510270292.5, by super
The step such as filter and nanofiltration UF membrane, extraction, preparation chromatogram and high pressure low temperature concentration prepares the raphanin that purity is higher than 99%, is used for
(3) step prepares raphanin dispersion.
(2) anhydrous ingredient powder is prepared
The commercially available powdered fructose of food or pharmaceutical grade, glucose, mannose, mannitol, D-sorbite etc. are placed in micro-
It is 0.06MPa in vacuum, temperature is prepared to be dried 1 hour under conditions of 40 DEG C in ripple low-temperature vacuum drying case
Anhydrous powder shape fructose, glucose, mannose, mannitol, D-sorbite, also known as anhydrous ingredient powder prepare radish for lower step
Plain dispersion.
(3) raphanin dispersion is prepared
After the completion of first two steps, the anhydrous ingredient powder quality that the radish pixel volume prepared according to (1) step is prepared with (2) step
The ratio between (L/kg) be 1: 5 ratio, first by (2) step prepare anhydrous ingredient powder put into grinding distribution machine in, grinding 5 minutes after
The raphanin again prepared by (1) step is put into, and is ground 15 minutes under conditions of rotating speed is 6000rpm, that is, is prepared raphanin
Dispersion, prepares for lower step and stabilizes raphanin.
(4) prepare and stabilize raphanin
After the completion of (3) step, the raphanin dispersion that (3) step is prepared is sub-packed in the brown glass that volume is 10mL
In bottle, bottle stopper for lyophilized powder formulation is placed, then be placed in low temperature refrigerator, be put into the vacuum with pressing device after precooling 5h at -40 DEG C cold
In lyophilizer, temperature be -50 DEG C, vacuum be to be freeze-dried 24h under 20Pa, nitrogen is then passed through into freeze drier
Gas, is continued with 15 minutes, last gland sealing, that is, prepares stabilisation raphanin.It is placed on the condition that temperature is -20 DEG C
Under be kept in dark place December, raphanin purity remains at more than 99%.
Embodiment 2
A kind of to prepare the method for stabilizing raphanin, its concrete technology step is as follows：
(1) raphanin is prepared
Using the existing technology for preparing raphanin, the method introduced according to Patent No. 201510270292.5, by super
The step such as filter and nanofiltration UF membrane, extraction, preparation chromatogram and high pressure low temperature concentration prepares the raphanin that purity is higher than 99%, is used for
(3) step prepares raphanin dispersion.
(2) anhydrous ingredient powder is prepared
The commercially available powdered fructose of food or pharmaceutical grade, glucose, mannose, mannitol, D-sorbite etc. are placed in micro-
It is 0.075MPa in vacuum, temperature is prepared to be dried 2 hours under conditions of 42 DEG C in ripple low-temperature vacuum drying case
Go out anhydrous powder shape fructose, glucose, mannose, mannitol, D-sorbite, also known as anhydrous ingredient powder prepares Lay for lower step
Fu element dispersions.
(3) raphanin dispersion is prepared
After the completion of first two steps, the anhydrous ingredient powder quality that the radish pixel volume prepared according to (1) step is prepared with (2) step
The ratio between (L/kg) be 1: 7 ratio, first by (2) step prepare anhydrous ingredient powder put into grinding distribution machine in, grinding 7 minutes after
The raphanin again prepared by (1) step is put into, and is ground 25 minutes under conditions of rotating speed is 7500rpm, that is, is prepared raphanin
Dispersion, prepares for lower step and stabilizes raphanin.
(4) prepare and stabilize raphanin
After the completion of (3) step, the raphanin dispersion that (3) step is prepared is sub-packed in the brown glass that volume is 10mL
In bottle, bottle stopper for lyophilized powder formulation is placed, then be placed in low temperature refrigerator, be put into the vacuum with pressing device after precooling 7h at -30 DEG C cold
In lyophilizer, temperature be -45 DEG C, vacuum be to be freeze-dried 36h under 35Pa, nitrogen is then passed through into freeze drier
Gas, is continued with 25 minutes, last gland sealing, that is, prepares stabilisation raphanin.Temperature is placed on under conditions of 4 DEG C
It is kept in dark place 13 months, raphanin purity remains at more than 99%.
Embodiment 3
A kind of to prepare the method for stabilizing raphanin, its concrete technology step is as follows：
(1) raphanin is prepared
Using the existing technology for preparing raphanin, the method introduced according to Patent No. 201510270292.5, by super
The step such as filter and nanofiltration UF membrane, extraction, preparation chromatogram and high pressure low temperature concentration prepares the raphanin that purity is higher than 99%, is used for
(3) step prepares raphanin dispersion.
(2) anhydrous ingredient powder is prepared
The commercially available powdered fructose of food or pharmaceutical grade, glucose, mannose, mannitol, D-sorbite etc. are placed in micro-
It is 0.09MPa in vacuum, temperature is prepared to be dried 3 hours under conditions of 45 DEG C in ripple low-temperature vacuum drying case
Anhydrous powder shape fructose, glucose, mannose, mannitol, D-sorbite, also known as anhydrous ingredient powder prepare radish for lower step
Plain dispersion.
(3) raphanin dispersion is prepared
After the completion of first two steps, the anhydrous ingredient powder quality that the radish pixel volume prepared according to (1) step is prepared with (2) step
The ratio between (L/kg) be 1: 10 ratio, first by (2) step prepare anhydrous ingredient powder put into grinding distribution machine in, grind 10 minutes
The raphanin for afterwards again preparing (1) step is put into, and is ground 30 minutes under conditions of rotating speed is 9000rpm, that is, is prepared radish
Plain dispersion, prepares for lower step and stabilizes raphanin.
(4) prepare and stabilize raphanin
After the completion of (3) step, the raphanin dispersion that (3) step is prepared is sub-packed in the brown glass that volume is 20mL
In bottle, bottle stopper for lyophilized powder formulation is placed, then be placed in low temperature refrigerator, be put into the vacuum with pressing device after precooling 10h at -20 DEG C cold
In lyophilizer, temperature be -40 DEG C, vacuum be to be freeze-dried 48h under 50Pa, nitrogen is then passed through into freeze drier
Gas, is continued with 30 minutes, last gland sealing, that is, prepares stabilisation raphanin.Temperature is placed on under conditions of 4 DEG C
It is kept in dark place 14 months, raphanin purity remains at more than 99%.
Claims (1)
1. a kind of prepare the method for stabilizing raphanin, it is characterised in that specific processing step is as follows：
(1) raphanin is prepared
Using the existing technology for preparing raphanin, the method introduced according to Patent No. 201510270292.5, by ultrafiltration and
Nanofiltration UF membrane, extraction, prepare the step such as chromatogram and high pressure low temperature concentration and prepare the raphanin that purity is higher than 99%, for the
(3) step prepares raphanin dispersion；
(2) anhydrous ingredient powder is prepared
The commercially available powdered fructose of food or pharmaceutical grade, glucose, mannose, mannitol, D-sorbite etc. are placed in microwave low
It is 0.06MPa in vacuum, temperature is prepared anhydrous to be dried 1 hour under conditions of 40 DEG C in warm vacuum drying chamber
Powdered fructose, glucose, mannose, mannitol, D-sorbite, also known as anhydrous ingredient powder prepare raphanin point for lower step
Dissipate thing；
(3) raphanin dispersion is prepared
After the completion of first two steps, the anhydrous ingredient powder mass ratio that the radish pixel volume prepared according to (1) step is prepared with (2) step
(L/kg) ratio for being 1: 5, the anhydrous ingredient powder for first preparing (2) step is put into grinding distribution machine, and grinding again will after 5 minutes
Raphanin input prepared by (1) step, grinds 15 minutes under conditions of rotating speed is 6000rpm, that is, prepares raphanin and disperse
Thing, prepares for lower step and stabilizes raphanin；
(4) prepare and stabilize raphanin
After the completion of (3) step, the raphanin dispersion that (3) step is prepared is sub-packed in the Brown Glass Brown glass bottles and jars only that volume is 10mL
In, bottle stopper for lyophilized powder formulation is placed, then be placed in low temperature refrigerator, the vacuum refrigeration with pressing device is put into after precooling 5h at -40 DEG C
In drying machine, temperature be -50 DEG C, vacuum be to be freeze-dried 24h under 20Pa, nitrogen is then passed through into freeze drier,
Continue with 15 minutes, last gland sealing prepares stabilisation raphanin, is placed on temperature under conditions of -20 DEG C
It is kept in dark place December, raphanin purity remains at more than 99%.
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| CN201710491907.6A CN107162947A (en) | 2017-06-15 | 2017-06-15 | It is a kind of to prepare the method for stabilizing raphanin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115708538A (en) * | 2023-01-09 | 2023-02-24 | 苏陀科技(北京)有限公司 | Preparation method of modified soybean protein isolate and modified soybean protein isolate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012010587A1 (en) * | 2010-07-23 | 2012-01-26 | Auriga International | Sulforaphane stabilization |
| CN102688219A (en) * | 2012-02-03 | 2012-09-26 | 马建华 | Sulforaphane microencapsulation method |
| FR2977161A1 (en) * | 2011-07-01 | 2013-01-04 | Sojasun Technologies | COMPOSITIONS FOR THE TREATMENT OR PREVENTION OF PROSTATE CANCER BASED ON BROCOLI SEED EXTRACT. |
| CN104720072A (en) * | 2015-02-15 | 2015-06-24 | 北京化工大学 | Method for improving sulforaphene stability |
| CN105410302A (en) * | 2015-10-29 | 2016-03-23 | 广州六顺生物科技有限公司 | Tabletted sweets containing sulforaphen as well as preparation method and application of tabletted sweets |
-
2017
- 2017-06-15 CN CN201710491907.6A patent/CN107162947A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012010587A1 (en) * | 2010-07-23 | 2012-01-26 | Auriga International | Sulforaphane stabilization |
| CN103079545B (en) * | 2010-07-23 | 2015-12-02 | 奥里格国际股份有限公司 | The stabilisation of sulforaphane |
| FR2977161A1 (en) * | 2011-07-01 | 2013-01-04 | Sojasun Technologies | COMPOSITIONS FOR THE TREATMENT OR PREVENTION OF PROSTATE CANCER BASED ON BROCOLI SEED EXTRACT. |
| CN102688219A (en) * | 2012-02-03 | 2012-09-26 | 马建华 | Sulforaphane microencapsulation method |
| CN104720072A (en) * | 2015-02-15 | 2015-06-24 | 北京化工大学 | Method for improving sulforaphene stability |
| CN105410302A (en) * | 2015-10-29 | 2016-03-23 | 广州六顺生物科技有限公司 | Tabletted sweets containing sulforaphen as well as preparation method and application of tabletted sweets |
Non-Patent Citations (2)
| Title |
|---|
| STEPHEN J.FRANKLIN: "Stability of sulforaphane for topical formulation", 《DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY》 * |
| 田桂芳: "天然产物莱菔素的降解机理及稳定性研究", 《中国博士学位论文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115708538A (en) * | 2023-01-09 | 2023-02-24 | 苏陀科技(北京)有限公司 | Preparation method of modified soybean protein isolate and modified soybean protein isolate |
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