CN106860923A - A kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active - Google Patents
A kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active Download PDFInfo
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- CN106860923A CN106860923A CN201710055208.7A CN201710055208A CN106860923A CN 106860923 A CN106860923 A CN 106860923A CN 201710055208 A CN201710055208 A CN 201710055208A CN 106860923 A CN106860923 A CN 106860923A
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- 210000001789 adipocyte Anatomy 0.000 title claims abstract description 104
- 238000002054 transplantation Methods 0.000 title claims abstract description 33
- 230000000694 effects Effects 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 239000012930 cell culture fluid Substances 0.000 claims abstract description 21
- 239000007864 aqueous solution Substances 0.000 claims description 46
- 239000000203 mixture Substances 0.000 claims description 33
- 239000001963 growth medium Substances 0.000 claims description 31
- 239000003963 antioxidant agent Substances 0.000 claims description 22
- 239000004606 Fillers/Extenders Substances 0.000 claims description 20
- 230000003078 antioxidant effect Effects 0.000 claims description 20
- 235000006708 antioxidants Nutrition 0.000 claims description 20
- 210000004027 cell Anatomy 0.000 claims description 18
- 239000011229 interlayer Substances 0.000 claims description 16
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 15
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 14
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
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- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 claims description 6
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- 238000007789 sealing Methods 0.000 claims description 5
- QAQJMLQRFWZOBN-UHFFFAOYSA-N 2-(3,4-dihydroxy-5-oxo-2,5-dihydrofuran-2-yl)-2-hydroxyethyl hexadecanoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)C1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-UHFFFAOYSA-N 0.000 claims description 4
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims 1
- 238000005984 hydrogenation reaction Methods 0.000 claims 1
- 239000000787 lecithin Substances 0.000 claims 1
- 229940067606 lecithin Drugs 0.000 claims 1
- 235000010445 lecithin Nutrition 0.000 claims 1
- 238000002347 injection Methods 0.000 abstract description 24
- 239000007924 injection Substances 0.000 abstract description 24
- 238000000605 extraction Methods 0.000 abstract description 6
- 230000006641 stabilisation Effects 0.000 abstract description 3
- 238000011105 stabilization Methods 0.000 abstract description 3
- 239000003925 fat Substances 0.000 description 33
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 16
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 7
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical group CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 7
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- 239000003795 chemical substances by application Substances 0.000 description 2
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- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 1
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
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- 238000004458 analytical method Methods 0.000 description 1
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- AIFCFBUSLAEIBR-UHFFFAOYSA-N ginkgetin Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C(C=1)=CC=C(OC)C=1C1=C(O)C=C(O)C(C(C=2)=O)=C1OC=2C1=CC=C(O)C=C1 AIFCFBUSLAEIBR-UHFFFAOYSA-N 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
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- A61M1/64—Containers with integrated suction means
- A61M1/67—Containers incorporating a piston-type member to create suction, e.g. syringes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/3145—Filters incorporated in syringes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
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- A61B2017/00792—Plastic surgery
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- A—HUMAN NECESSITIES
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- A61M2210/00—Anatomical parts of the body
- A61M2210/10—Trunk
- A61M2210/1007—Breast; mammary
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/35—Polyols, e.g. glycerin, inositol
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
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Abstract
The present invention proposes a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active, including needle guard, syringe needle and syringe body, the syringe body includes syringe and plunger, the syringe front end is provided with the needle tubing being connected with the syringe needle, the syringe is provided with scale, the screen pack for being fixed on syringe inwall is provided with the syringe, the syringe is provided with cell culture fluid reservoir, there is adipocyte culture liquid in the cell culture fluid reservoir, the cell culture fluid reservoir passes through the first magnetic valve UNICOM with the syringe.The present invention provides the multi-function device that a kind of fat cell is extracted, analyzes and inject one, the environment of the short-term holding fat cell vigor of stabilization can be provided simultaneously, can implement, the once extraction of fat cell, a small amount of multiple injection, the step of repeatedly extracting is reduced, a small amount of multiple injection effect is also improved.
Description
Technical field
It is more particularly to a kind of short-term to maintain the multi-functional from body fat of fat cell activity the invention belongs to surgical instruments field
Fat transplantation device.
Background technology
Autologous fat transplantation art, refers to by the more rich position of human body own fat, such as abdomen, stern, thigh or upper arm etc.
Fat, suctioned out with vacuum sliding chute method, by after specially treated or pure lipochondrion, injection implantation needs that what is changed have scarce
In Xian Shou areas, to change a kind of operation method improved by area's form.Mostly comparing at present is autologous fat chest enlarge, by body
Upper superabundant fats cell transplantation is expelled to chest.Autologous fat transplantation is the lipochondrion of itself, will not also be produced immune anti-
Should and rejection, autologous fat transplantation will not cause the change of the endocrinal environment of human body, wound will not be produced in itself to mammary gland
Evil, to fertility from now on, lactation, does not have bad influence.Simultaneously autologous fat transplantation materials be easier, to local fat compared with
The people for piling up, except doing while face modification, chest enlarge, may also function as weight-reducing, mould perfect concavo-convex body curvature more
Effect.
Autologous fat transplantation art, is included in the extraction of negative pressure fat cell, the fat cell of fat cell long position substantially
Filtering and require supplementation with fat cell region fat cell inject three steps.It is clear that CN2865698Y discloses a kind of fat
Purifier is washed, is made up of with filter dish is crossed Washing cup, can filtered and dispose destroyed fat.The problem of presence is, it is necessary to tight
The aseptic environment of lattice could be implemented, and easily pollute external microbe.CN103598901A discloses a kind of fat transplantation device, bag
Filter screen, liposuction pipe and fat injection pipe are included, fat, through filter screen filtration, realizes fat after extraction in same device
Extract, filtering and fat implantation, it is to avoid cause outside contamination problem in transfer process, the problem for existing is, including can only lean on
Fat cell deadweight carries out filtration step, and filter efficiency is low, and the fat cell of partial crushing can not be filtered out effectively, while mistake
Filtering velocity degree is slow, influences follow-up injecting step, is also exactly that the fat cell for obtaining can not be preserved, it is necessary to all be expelled to immediately
The position that need to be transplanted.In practice, it has been found that during injection in fat cell transplanting, repeatedly a small amount of mode can be more for fat cell
Good is moulding, and existing apparatus cannot meet and be actually needed.
The content of the invention
Regarding to the issue above, the present invention proposes a kind of short-term multi-functional autologous fat transplantation for maintaining fat cell active
Device, it is possible to achieve the extraction of fat cell, the separation of fat cell and fat cell activity maintaining function, can facilitate, pacify
Entirely, multiple a small amount of injection of fat cell is effectively carried out, it is specific as follows：
A kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active, including needle guard, syringe needle and injection
Device body, the syringe body includes syringe and plunger, and the syringe front end is provided with the needle tubing being connected with the syringe needle, described
Syringe is provided with scale, and the screen pack for being fixed on syringe inwall is provided with the syringe, and the fat transplantation device also includes logical
The cell culture fluid reservoir that the first magnetic valve is connected with the syringe is crossed, has fat cell in the cell culture fluid reservoir
Nutrient solution.
Optionally, the fat transplantation device also includes the cell cleaning fluid connected with the syringe by the second magnetic valve
Reservoir, has cell cleaning fluid in the cell cleaning fluid reservoir.
Optionally, the plunger front end is provided with sealing ring.
Optionally, the needle tubing is provided with scale.
Optionally, the syringe is provided with interlayer, and the interlayer is provided with opening.
Optionally, the screen pack is 40-50 mesh.
Optionally, the adipocyte culture liquid be will culture composition and antioxidant to be dissolved in DMEM culture mediums water-soluble
Liquid is prepared from, and concentration of the culture composition in the DEME culture medium aqueous solution is 50-200mg/ml, the antioxidant
Concentration in the DEME culture medium aqueous solution is 0.1-1.0mg/ml, and the concentration of the DMEM culture mediums aqueous solution is 5-
10mg/mL, the culture composition it is main by following parts by weight into being grouped into：Resveratrol 1-10 parts, the double Huangs of different ginkgo
Ketone 0.1-1 parts, hydrolecithin 0.1-1 parts and maltitol 1-5 parts.Optionally, the antioxidant is that ratio of weight and number is
2:1 ascorbyl palmitate and vitamin C.
Optionally, the adipocyte culture liquid also includes energy extender, and the energy extender is in DEME culture mediums
Concentration in the aqueous solution is 0.1-1.0 μ g/ml, the energy extender it is main by following parts by weight into being grouped into：It is as follows
The each component of parts by weight：Phosphatidyl serine 0.1-1 parts, Sodium Pyruvate 5-8 parts, fumaric acid 1-2 parts, palmitamide 2-8
Part, potassium dihydrogen phosphate 1-5 parts.
Optionally, the cell cleaning fluid is physiological saline.
A kind of embodiment of the invention provides the multi-function device that a kind of fat cell is extracted, analyzes and inject one, this
Another embodiment of invention can provide the environment of the short-term holding fat cell vigor of stabilization, it is possible to achieve fat cell
Once extract, a small amount of multiple injection, reduce the step of repeatedly extracting, a small amount of multiple injection effect is also improved.
Brief description of the drawings
The syringe body section of structure of Fig. 1 embodiment of the present invention 1；
The syringe body section of structure of Fig. 2 embodiment of the present invention 2；
In figure, 1 syringe, 2 plungers, 3 needle tubings 4, screen pack, 5 cell culture fluid reservoirs, 6 first magnetic valves, 7 cells are clear
Washing lotion reservoir, 8 second magnetic valves, 9 sealing rings, 10 interlayers, 11 interlayer openings.
Specific embodiment
Embodiment 1
A kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active, as shown in figure 1, a kind of short-term dimension
Hold the multi-functional autologous fat transplantation device of fat cell activity, including needle guard, syringe needle and syringe body, the syringe sheet
Body includes syringe 1 and plunger 2, and the front end of the syringe 1 is provided with the needle tubing 3 being connected with the syringe needle, and the syringe 1 is provided with quarter
The screen pack 4 for being fixed on the inwall of syringe 1 is provided with degree, the syringe 1, the fat transplantation device also includes passing through the first electromagnetism
There is fat cell to train in the cell culture fluid reservoir 5 that the UNICOM of valve 6 connects with the syringe 1, the cell culture fluid reservoir 5
Nutrient solution.
Before use, the multi-functional autologous fat transplantation device of the short-term maintenance fat cell activity of the present embodiment needs elder generation
Carry out plunger 2 to adjust, its position is located at syringe 1, the first magnetic valve 6 is closed, and pillow is connected on syringe body,
Needle guard is enclosed within pillow, is sterilized.After the sterilization of liposuction position is cut, needle guard, needle contact fat cell, along pin are removed
Cylinder 1 rearwardly pulls plunger 2, extracts fat cell and enters on the arrival screen pack 4 of syringe 1 by needle tubing 3.Extract fat cell complete
Cheng Hou, puts needle guard, opens the first magnetic valve 6, adjustment space position so that adipocyte culture liquid enters in syringe 1, passes through
Screen pack 4 is contacted with fat cell, and by suspending repeatedly and filtering adipose cells, the incomplete fat for isolating destruction is thin
Born of the same parents.During injection fat cell, adjustment space position so that adipocyte culture liquid enters in cell culture fluid reservoir 5 is closed
First magnetic valve 6.Plunger 2 is promoted to head along syringe 1, is promoted fat cell to be reached by needle tubing 3 and syringe needle and is subscribed injection part
Position.By the scale on syringe 1, the volume of fat cell, auxiliary can be calculated when extracting fat cell and injection fat cell
Extract and injection process completes to judge.The need for for repeatedly a small amount of injection fat cell, can be realized by fractional injection.
For the first time after injection fat cell, needle guard is put, open the first magnetic valve 6, adjustment space position so that adipocyte culture liquid
Into in syringe 1, contacted with fat cell through screen pack 4, the adipocyte culture liquid environment good for fat cell is provided,
Fat cell vigor is improved, extends the fat cell holding time.During to second injection fat cell, adjustment space position makes
Obtain adipocyte culture liquid to enter in cell culture fluid reservoir 5, close the first magnetic valve 6.Along syringe 1 plunger is promoted to head
2, promote fat cell to be reached by needle tubing 3 and syringe needle and subscribe injection site.Later multiple injection step is analogized.
The present embodiment is extracted except providing a kind of fat cell, the multi-function device of analysis and injection one, while can be with
The environment of the short-term holding fat cell vigor of stabilization is provided, it is possible to implement, the once extraction of fat cell, a small amount of repeatedly note
Penetrate, reduce the step of repeatedly extracting, a small amount of multiple injection effect is also improved.
Embodiment 2
Difference with embodiment 1 is that the fat transplantation device also includes passing through the second magnetic valve 8 and the pin
The cell cleaning fluid reservoir 7 of the connection of cylinder 1, has cell cleaning fluid in the cell cleaning fluid reservoir 7.The front end of the plunger 2
It is provided with sealing ring 9.The needle tubing 3 is provided with scale.The syringe 1 is provided with interlayer 10, and the interlayer is provided with opening 11.It is described
Screen pack 4 is 40-50 mesh.The cell cleaning fluid is physiological saline.
Cell cleaning fluid reservoir and the second magnetic valve 8 in the present embodiment can realize to fat cell inject before it is clear
Wash, remove the cell culture fluid in fat cell, it is to avoid subsidiary cell culture fluid is produced to human body during injection fat cell
Adverse effect.Before fat cell injection, adjustment space position so that adipocyte culture liquid enters cell culture fluid reservoir 5
It is interior, close the first magnetic valve 6.Then the second magnetic valve 8, adjustment adjustment space position so that physiological saline is fully contacted are opened
Fat cell, except the cell culture fluid of attachment removal.Then adjustment space position so that physiological saline enters, into cell cleaning
In liquid reservoir 7, the second magnetic valve 8 is closed, then injected.
The front end of the plunger 2 is provided with sealing ring 9, can strengthen the seal of plunger 2 and syringe 1.The needle tubing 3 is provided with
Scale, needle tubing 3 is thinner than syringe 1, therefore scale unit on needle tubing 3 is more accurate than scale on syringe 1, can be more accurate it is right
Fat cell is quantified.The syringe 1 is provided with interlayer 10, and the interlayer is provided with opening 11, can be added by opening 11
Trash ice creates low temperature environment to interlayer 10, is beneficial to maintain fat cell activity.
Embodiment 3
Difference with embodiment 2 is that the adipocyte culture liquid in cell culture fluid reservoir 5 is will to cultivate group
Compound and antioxidant are dissolved in the DMEM culture medium aqueous solution and are prepared from, and culture composition is in the DEME culture medium aqueous solution
Concentration is 50mg/ml, and concentration of the antioxidant in the DEME culture medium aqueous solution is 0.1mg/ml, and the DMEM culture mediums are water-soluble
The concentration of liquid is 5mg/mL, the culture composition it is main by following parts by weight into being grouped into：1 part of resveratrol, different silver
1 part of 1 part of ginkgetin, 0.1 part of hydrolecithin and maltitol；It is ascorbyl palmitate that the antioxidant is.
Embodiment 4
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 200mg/
Ml, concentration of the antioxidant in the DEME culture medium aqueous solution is 1.0mg/ml, and the concentration of the DMEM culture mediums aqueous solution is
10mg/mL, the culture composition it is main by following parts by weight into being grouped into：10 parts of resveratrol, Isoginkgetin 1
5 parts of part, 0.1 part of hydrolecithin and maltitol；The antioxidant is vitamin C.
Embodiment 5
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
Ml, concentration of the antioxidant in the DEME culture medium aqueous solution is respectively 0.75mg/ml, the concentration of the DMEM culture mediums aqueous solution
Be 10mg/mL, the culture composition it is main by following parts by weight into being grouped into：5 parts of resveratrol, Isoginkgetin
1 part of 0.5 part, 0.1 part of hydrolecithin and maltitol；The antioxidant is that ratio of weight and number is 2:1 ascorbic acid palm fibre
Glycerin monostearate and ascorbic mixture.
Embodiment 6
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
Ml, concentration of the antioxidant in the DEME culture medium aqueous solution is respectively 0.75mg/ml.The DMEM culture mediums aqueous solution it is dense
It is 10mg/mL to spend, the culture composition it is main by following parts by weight into being grouped into：5 parts of resveratrol, different ginkgo are double yellow
1 part of 0.5 part of ketone, 0.1 part of hydrolecithin and maltitol；The antioxidant is that ratio of weight and number is 2:1 ascorbic acid
Palmitate and ascorbic mixture；The adipocyte culture liquid also includes energy extender, and the energy extender exists
Concentration in the DEME culture medium aqueous solution is 0.1 μ g/ml, and the energy extender is main by following parts by weight into packet
Into：0.1 part of phosphatidyl serine, 5 parts of Sodium Pyruvate, 1 part of fumaric acid, 2 parts of palmitamide, 1 part of potassium dihydrogen phosphate.
Embodiment 7
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
The concentration of ml, ascorbyl palmitate and vitamin C in the DEME culture medium aqueous solution is respectively 0.5mg/ml and 0.25mg/
ml.The concentration of the DMEM culture mediums aqueous solution is 10mg/mL, the culture composition it is main by following parts by weight into
It is grouped into：1 part of 5 parts of resveratrol, 0.5 part of Isoginkgetin, 0.1 part of hydrolecithin and maltitol；It is described anti-oxidant
Agent is that ratio of weight and number is 2:1 ascorbyl palmitate and ascorbic mixture；The adipocyte culture liquid is also wrapped
Energy extender is included, concentration of the energy extender in the DEME culture medium aqueous solution is 1.0 μ g/ml, the energy supplement
Agent it is main by following parts by weight into being grouped into：1 part of phosphatidyl serine, 8 parts of Sodium Pyruvate, 2 parts of fumaric acid, palmityl
8 parts of amine, 5 parts of potassium dihydrogen phosphate.
Embodiment 8
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
The concentration of ml, ascorbyl palmitate and vitamin C in the DEME culture medium aqueous solution is respectively 0.5mg/ml and 0.25mg/
ml.The concentration of the DMEM culture mediums aqueous solution is 10mg/mL, the culture composition it is main by following parts by weight into
It is grouped into：1 part of 5 parts of resveratrol, 0.5 part of Isoginkgetin, 0.1 part of hydrolecithin and maltitol；It is described anti-oxidant
Agent is that ratio of weight and number is 2:1 ascorbyl palmitate and ascorbic mixture；The adipocyte culture liquid is also wrapped
Energy extender is included, concentration of the energy extender in the DEME culture medium aqueous solution is 0.5 μ g/ml, the energy supplement
Agent it is main by following parts by weight into being grouped into：0.5 part of phosphatidyl serine, 5 parts of Sodium Pyruvate, 2 parts of fumaric acid, palm
5 parts of acid amides, 1 part of potassium dihydrogen phosphate.
Reference examples 1
Difference with embodiment 3 is that adipocyte culture liquid is physiological saline.
Reference examples 2
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 50mg/
Ml, concentration of the antioxidant in the DEME culture medium aqueous solution is 0.1mg/ml, and the concentration of the DMEM culture mediums aqueous solution is
5mg/mL, the culture composition it is main by following parts by weight into being grouped into：1 part of resveratrol, Isoginkgetin 1
Part；It is ascorbyl palmitate that the antioxidant is.
Reference examples 3
Difference with embodiment 3 is that concentration of the culture composition in the DEME culture medium aqueous solution is 50mg/
Ml, concentration of the antioxidant in the DEME culture medium aqueous solution is 1.0mg/ml, and the concentration of the DMEM culture mediums aqueous solution is
10mg/mL, concentration of the culture composition in the DEME culture medium aqueous solution is 50mg/ml, and the culture composition is main by such as
Lower parts by weight into being grouped into：1 part of Isoginkgetin, 0.1 part of hydrolecithin；It is vitamin C that the antioxidant is.
Reference examples 4
Difference with embodiment 5 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
The concentration of ml, ascorbyl palmitate and vitamin C in the DEME culture medium aqueous solution be respectively 0.5mgl/ml and
0.25mg/ml, the concentration of the DMEM culture mediums aqueous solution is 10mg/mL, and the culture composition is main by following weight
Number into being grouped into：1 part of 5 parts of resveratrol, 0.5 part of Isoginkgetin, 0.1 part of hydrolecithin and maltitol；Institute
Stating adipocyte culture liquid also includes energy extender, and concentration of the energy extender in the DEME culture medium aqueous solution is
0.1 μ g/ml, the energy extender it is main by following parts by weight into being grouped into：The each component of following parts by weight：Phosphide
0.1 part of acyl serine, 5 parts of Sodium Pyruvate, 1 part of fumaric acid.
Reference examples 5
Difference with embodiment 5 is that concentration of the culture composition in the DEME culture medium aqueous solution is 100mg/
The concentration of ml, ascorbyl palmitate and vitamin C in the DEME culture medium aqueous solution is respectively 0.5mg/ml and 0.25mg/
Ml, the concentration of the DMEM culture mediums aqueous solution is 10mg/mL, the culture composition it is main by following parts by weight into
It is grouped into：1 part of 5 parts of resveratrol, 0.5 part of Isoginkgetin, 0.1 part of hydrolecithin and maltitol；The fat is thin
Born of the same parents' nutrient solution also includes energy extender, and concentration of the energy extender in the DEME culture medium aqueous solution is 0.1 μ g/ml,
The energy extender it is main by following parts by weight into being grouped into：The each component of following parts by weight：2 parts of palmitamide,
1 part of potassium dihydrogen phosphate.
Influence of the cell culture fluid of experimental example 1 to fat cell activity
Filled using the multi-functional autologous fat transplantation of the short-term maintenance fat cell activity of reference examples 1-5 and embodiment 3-8
Put, determined for the ease of cell viability, the filtering function of temporary close device, specifically, covering one layer of PET nucleopore on screen pack
Film, the thickness of nucleopore membranes is 8-35um, and nucleopore aperture is 15nm-8um, and cell culture fluid can be passed freely through, fat cell and
Fragment cannot pass through.After extracting cell, per 1h cell culture fluid suspension 10min.Suspend every time after terminating, take 1ml fat thin
Born of the same parents, cell count is carried out with Countess II FL.Fat cell activity is to preserve the adipocyte count after the regular period to account for
The percentage of the adipocyte count of 0h (not preserving directly counting after fat cell extraction).
Influence of the cell culture fluid of table 1 to fat cell activity
As it can be seen from table 1 embodiment 3-5 fat cell activity is significantly better than reference examples 1-3, P<0.05, wherein with reality
Example 5 is applied for optimal, is shown suitable culture composition, the DEME culture mediums aqueous solution, antioxidant, can significantly be provided fat cell
Vigor, extend storage time；Embodiment 6-8 is significantly better than reference examples 4-5, P<0.05, wherein being optimal, reality with embodiment 8
The implementation method fat cell vigor for applying example 8 still can reach 74% after 72h, show energy extender phosphatidyl serine,
Sodium Pyruvate, fumaric acid, palmitamide, potassium dihydrogen phosphate can significantly provide the vigor of fat cell, extend storage time,
For the fractional injection of fat cell provides good basis.
Influence of the low temperature environment of the interlayer trash ice of experimental example 2 manufacture to fat cell activity
Trash ice is not added with as control group with the interlayer of embodiment 3, it is experimental group that interlayer is on the rocks.Every group of 5 parallel sampleses.Using
The cell viability collection of experimental example 1 and assay method.
Influence of the low temperature environment of the interlayer trash ice of table 2 manufacture to fat cell activity
From table 2 it can be seen that experimental group fat cell activity is significantly better than control group, P<Control group fat after 0.05,12h
Cytoactive only has 44%, and control group can reach 74%, shows that the low temperature environment that trash ice interlayer is created can be carried significantly
For the vigor of fat cell, further extend storage time.
These are only embodiments of the invention.For a person skilled in the art, it is easy to draped over one's shoulders according to above-mentioned
The spirit of dew makes various changes and change to these embodiments.These changes and change are all contained in the application claims
Within the scope of restriction.
Claims (10)
1. it is a kind of it is short-term maintain fat cell activity multi-functional autologous fat transplantation device, it is characterised in that including needle guard, pin
Head and syringe body, the syringe body include syringe (1) and plunger (2), and syringe (1) front end is provided with and the pin
The needle tubing (3) of head connection, the syringe (1) is provided with scale, the syringe (1) and is provided with the mistake for being fixed on syringe (1) inwall
Filter screen (4), the fat transplantation device also includes the cell culture fluid connected with the syringe (1) by the first magnetic valve (6)
There is adipocyte culture liquid in reservoir (5), the cell culture fluid reservoir (5).
2. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, the fat transplantation device also includes that the cell cleaning fluid connected with the syringe (1) by the second magnetic valve (8) is stored up
There is cell cleaning fluid in liquid bath (7), the cell cleaning fluid reservoir (7).
3. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, plunger (2) front end is provided with sealing ring (9).
4. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, the needle tubing (3) is provided with scale.
5. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, the syringe (1) is provided with interlayer (10), the interlayer is provided with opening (11).
6. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, the screen pack (4) is 40-50 mesh.
7. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 1, it is special
Levy and be, the adipocyte culture liquid be will culture composition and antioxidant be dissolved in the DMEM culture mediums aqueous solution prepare and
Into concentration of the culture composition in the DEME culture medium aqueous solution is 50-200mg/ml, and the antioxidant is trained in DEME
It is 0.1-1.0mg/ml to support the concentration in the base aqueous solution, and the concentration of the DMEM culture mediums aqueous solution is 5-10mg/mL, the training
Support composition it is main by following parts by weight into being grouped into：Resveratrol 1-10 parts, Isoginkgetin 0.1-1 parts, hydrogenation
Lecithin 0.1-1 parts and maltitol 1-5 parts.
8. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 7, it is special
Levy and be, the antioxidant is that ratio of weight and number is 2:1 ascorbyl palmitate and ascorbic mixture.
9. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 8, it is special
Levy and be, the adipocyte culture liquid also includes energy extender, and the energy extender is in the DEME culture medium aqueous solution
Concentration be 0.1-1.0 μ g/ml, the energy extender it is main by following parts by weight into being grouped into：Phosphatidyl serine
0.1-1 parts, Sodium Pyruvate 5-8 parts, fumaric acid 1-2 parts, palmitamide 2-8 parts, potassium dihydrogen phosphate 1-5 parts.
10. a kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell activity as claimed in claim 9, it is special
Levy and be, the cell cleaning fluid is physiological saline.
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| CN201710055208.7A CN106860923A (en) | 2017-01-24 | 2017-01-24 | A kind of short-term multi-functional autologous fat transplantation device for maintaining fat cell active |
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Application publication date: 20170620 |