Nothing Special   »   [go: up one dir, main page]

CN106834484A - A kind of carcinoma of the rectum external detection method - Google Patents

A kind of carcinoma of the rectum external detection method Download PDF

Info

Publication number
CN106834484A
CN106834484A CN201710109657.5A CN201710109657A CN106834484A CN 106834484 A CN106834484 A CN 106834484A CN 201710109657 A CN201710109657 A CN 201710109657A CN 106834484 A CN106834484 A CN 106834484A
Authority
CN
China
Prior art keywords
pcr method
fluorescent pcr
resolution
mutation detection
gene mutation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710109657.5A
Other languages
Chinese (zh)
Inventor
胡江海
张晓磊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningxia Haicheng Electronic Mdt Infotech Ltd
Original Assignee
Ningxia Haicheng Electronic Mdt Infotech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ningxia Haicheng Electronic Mdt Infotech Ltd filed Critical Ningxia Haicheng Electronic Mdt Infotech Ltd
Priority to CN201710109657.5A priority Critical patent/CN106834484A/en
Publication of CN106834484A publication Critical patent/CN106834484A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The global annual neopathy number of cases about 1,200,000 of colorectal cancer, more than 600,000, the incidence of disease occupies the 3rd case fatality rate and occupies the 4th number of dying of illness in year.In recent years, the incidence of disease of colorectal cancer was in obvious ascendant trend.Therefore, the treatment of colorectal cancer is always the focus of domestic and foreign scholars research.To ensure the accuracy and uniformity of pathological diagnosis result, the time being saved with manually, patient information being fed back into treatment doctor in time, the present invention provides a kind of carcinoma of the rectum external detection method.

Description

A kind of carcinoma of the rectum external detection method
Technical field
The present invention relates to vitro detection field, especially carcinoma of the rectum vitro detection field.
Background technology
The global annual neopathy number of cases about 1,200,000 of colorectal cancer, more than 600,000, the incidence of disease occupies the to number of dying of illness in year 3 case fatality rate occupy the 4th.In recent years, the incidence of disease of colorectal cancer was in obvious ascendant trend.Therefore, the treatment of colorectal cancer The always focus of domestic and foreign scholars research.With the western appropriate purport of target therapeutic agent EGF-R ELISA (EGFR) inhibitor The appearance of monoclonal antibody (Cetuximab) and Victibix (Panitumumab), when the treatment of colorectal cancer enters targeted therapy Generation.Multiple large samples, the prompting of III phase of multicenter clinical study results, the 2nd exon of KRAS genes the 12nd, 13 are close The mutation status of numeral and blocking EGFR monoclonal antibody --- Cetuximab, Victibix clear curative effect it is related, The patient of KRAS wild types can obtain maximized existence benefit from the treatment of Cetuximab and Victibix.It is lack of standardization Detection method will cause the anti-EGFR inhibitor for treating of part KRAS genic mutation type patient's acceptance errors, not only treat Effect on driving birds is not good, also greatly wastes the limited treatment window of patient and economic strength.
The content of the invention
To ensure the accuracy and uniformity of pathological diagnosis result, the time is saved with manually, in time feed back patient information To treatment doctor, the present invention provides a kind of carcinoma of the rectum external detection method.
Product of the present invention includes helicobacter pylori nucleic acid and its drug-tolerant gene mutation detection kit（High-resolution melts bent Line fluorescent PCR method）, K-Ras gene mutation detection kits（High-resolution fusion curve fluorescent PCR method）, EGFR genes dash forward Become detection kit（High-resolution fusion curve fluorescent PCR method）, CYP2C19 gene mutation detection kits（High-resolution melts Curve fluorescent PCR method）, ALDH2 gene mutation detection kits（High-resolution fusion curve fluorescent PCR method）, ABCG1 bases Because of mutation detection kit（High-resolution fusion curve fluorescent PCR method）, MDR1 gene mutation detection kits（High-resolution melts Solution curve fluorescent PCR method）, because mentioned reagent box composition, production technology etc. are similar to, therefore, product gene abrupt climatic change Kit（High-resolution fusion curve fluorescent PCR method）To represent, designed capacity is totally counted with 7 kinds of kits.
Description of the process：
Material storage is checked
The indexs such as base-pair, quality, purity, electrical conductivity, yield, oligo DNA concentration to raw material check, herein During can produce defective work S11, this step commission third party detection company detected.Defective work return to supplier.
Raw material weighing is configured
Weigh：Using liquid-transfering gun auxiliary electron balance to KODDNA polymerases, deoxyadenosine triphosphate, deoxycytidine triphosphate, Deoxyguanosine triphosphate, thymidine triphosphoric acid etc. are weighed.Raw material is configured to following four intermediate respectively：Enzymatic reagent （Contain the composition for intervening reaction）：Under normal temperature, mix KODDNA polymerases, desoxyadenossine three by a certain percentage on clean bench The raw materials such as phosphoric acid, deoxycytidine triphosphate, deoxyguanosine triphosphate, thymidine triphosphoric acid.Primer/probe reagent（Containing dry The composition of pre-reaction）：Under normal temperature, mix oligonucleotide 3- end primers and 5- ends primer in proportion on clean bench（Different item Mesh base-pair sequence is different）, remix Q-probe probes（Disparity items base-pair sequence is different）, add quantitative sulfuric acid Magnesium.
Positive quality control product：Under normal temperature, mix buffer and its detection project positive base-pair sequence in proportion on clean bench Row.
Negative quality-control product：Under normal temperature, mix buffer and its detection project feminine gender base-pair sequence in proportion on clean bench Row.
Product checking is to the susceptibility of product, correctness and repeatability index is sampled detection simultaneously, and detection is using complete Automatic nucleic acid purification and quantitative fluorescent PCR analysis system are to enzymatic reagent, primer/probe reagent, positive quality control product, negative Quality Control Corresponding sample carries out contrasting detection.
Brief description of the drawings：
Fig. 1 is process chart.

Claims (3)

1. the present invention provides a kind of carcinoma of the rectum external detection method.
2. product of the present invention includes helicobacter pylori nucleic acid and its drug-tolerant gene mutation detection kit（High-resolution fusion curve Fluorescent PCR method）, K-Ras gene mutation detection kits（High-resolution fusion curve fluorescent PCR method）, EGFR gene mutations Detection kit（High-resolution fusion curve fluorescent PCR method）, CYP2C19 gene mutation detection kits（High-resolution melts bent Line fluorescent PCR method）, ALDH2 gene mutation detection kits（High-resolution fusion curve fluorescent PCR method）, ABCG1 genes Mutation detection kit（High-resolution fusion curve fluorescent PCR method）, MDR1 gene mutation detection kits（High-resolution melts Curve fluorescent PCR method）.
3. right will require the technological process of 1 description：Material storage checks that raw material weighing is configured, positive quality control product, negative matter Control product, product examine is surveyed to the susceptibility of product, correctness and repeatability index is sampled detection simultaneously.
CN201710109657.5A 2017-02-27 2017-02-27 A kind of carcinoma of the rectum external detection method Pending CN106834484A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710109657.5A CN106834484A (en) 2017-02-27 2017-02-27 A kind of carcinoma of the rectum external detection method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710109657.5A CN106834484A (en) 2017-02-27 2017-02-27 A kind of carcinoma of the rectum external detection method

Publications (1)

Publication Number Publication Date
CN106834484A true CN106834484A (en) 2017-06-13

Family

ID=59134085

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710109657.5A Pending CN106834484A (en) 2017-02-27 2017-02-27 A kind of carcinoma of the rectum external detection method

Country Status (1)

Country Link
CN (1) CN106834484A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090202989A1 (en) * 2005-06-28 2009-08-13 Hillan Kenneth J Egfr and kras mutations
CN102115792A (en) * 2010-12-20 2011-07-06 苏州科贝生物技术有限公司 Method and kit for detecting KRAS gene mutations in human colon and rectum cancers
CN102465180A (en) * 2010-11-19 2012-05-23 上海基康生物技术有限公司 Method for detecting gene mutation of gene K-ras related to colorectal cancer and related kit

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090202989A1 (en) * 2005-06-28 2009-08-13 Hillan Kenneth J Egfr and kras mutations
CN102465180A (en) * 2010-11-19 2012-05-23 上海基康生物技术有限公司 Method for detecting gene mutation of gene K-ras related to colorectal cancer and related kit
CN102115792A (en) * 2010-12-20 2011-07-06 苏州科贝生物技术有限公司 Method and kit for detecting KRAS gene mutations in human colon and rectum cancers

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
PETER JO等: "Heterogeneity of KRAS Mutation Status in Rectal Cancer", 《PLOS ONE》 *
余元勋等: "《中国分子胃癌学》", 30 April 2016, 合肥：安徽科学技术出版社 *
宋伟庆等: "《胃癌》", 30 September 2007, 军事医学科学出版社 *
王倩等: "高分辨率熔解曲线分析法检测结直肠癌组织K-ras基因突变及其临床意义", 《医学研究生学报》 *
魏萍: "《临床医技新编》", 30 June 2014, 昆明：云南科技出版社 *

Similar Documents

Publication Publication Date Title
Gao et al. Before and after: comparison of legacy and harmonized TCGA genomic data commons’ data
Macintyre et al. Sequencing structural variants in cancer for precision therapeutics
Bar et al. Large-scale analysis of loss of imprinting in human pluripotent stem cells
Brennecke et al. Accounting for technical noise in single-cell RNA-seq experiments
Wang et al. Prediction of competing endogenous RNA coexpression network as prognostic markers in AML
CN102409088B (en) Method for detecting gene copy number variation
Wang et al. Validation of copy number variation sequencing for detecting chromosome imbalances in human preimplantation embryos
Roloff et al. Technical advances in the measurement of residual disease in acute myeloid leukemia
CN107849607A (en) The single-molecule sequencing of plasma dna
Liu et al. A comprehensive catalogue of regulatory variants in the cattle transcriptome
CN105368957A (en) Micro-droplet digital PCR (polymerase chain reaction) quantitative determination method of CHO (Chinese hamster ovary) cell gene copy number
CN108330546A (en) A kind of library constructing method and reagent of simplification
CN110408612B (en) Protective agent for low-concentration DNA standard substance, preservation method and application
Shi et al. Circular RNA expression profiling by microarray—A technical and practical perspective
CN106834484A (en) A kind of carcinoma of the rectum external detection method
Wallace et al. BARCODE-ALL: accelerated and cost-effective genetic risk stratification in acute leukemia using spectrally addressable liquid bead microarrays
Li et al. Development of a novel reference plasmid for accurate quantification of genetically modified Kefeng6 rice DNA in food and feed samples
Liu et al. Simple copy number determination with reference query pyrosequencing (RQPS)
CN108103178A (en) The high-throughput detection kit and detection method of neoplastic hematologic disorder fusion
CN112592972B (en) Early screening method and kit for diffuse toxic goiter susceptibility genes
Sun et al. The impact of digital DNA counting technologies on noninvasive prenatal testing
Wolter et al. Detection of miRNA targets in high-throughput using the 3'LIFE assay
Liu et al. Quality control of next-generation sequencing-based in vitro diagnostic test for onco-relevant mutations using multiplex reference materials in plasma
CN103103280A (en) Quick and batch test method for copy number of multi-copy genes of genomes
Hu et al. A novel multiplex rt-PCR assay for the detection of four chromosomal translocations of leukemia

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20170613