CN106770086A - The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument - Google Patents
The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument Download PDFInfo
- Publication number
- CN106770086A CN106770086A CN201611052722.7A CN201611052722A CN106770086A CN 106770086 A CN106770086 A CN 106770086A CN 201611052722 A CN201611052722 A CN 201611052722A CN 106770086 A CN106770086 A CN 106770086A
- Authority
- CN
- China
- Prior art keywords
- fluorescence
- light
- real
- quantitative pcr
- pcr instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 22
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 14
- 230000003287 optical effect Effects 0.000 claims abstract description 53
- 238000001914 filtration Methods 0.000 claims abstract description 36
- 238000001228 spectrum Methods 0.000 claims abstract description 13
- 239000000835 fiber Substances 0.000 claims abstract description 11
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000739 chaotic effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 230000005622 photoelectricity Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010359 gene isolation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/02—Details
- G01J1/04—Optical or mechanical part supplementary adjustable parts
- G01J1/0407—Optical elements not provided otherwise, e.g. manifolds, windows, holograms, gratings
- G01J1/0477—Prisms, wedges
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Optics & Photonics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
This application discloses a kind of fluorescence detection method of real-time fluorescence quantitative PCR instrument, including：LED light source sends light；The light incidence optical filtering forms exciting light after filtering；The exciting light enters incident optical；The light that incident optical sends is projected directly at the sample in sample cell；The fluorescence that sample is excited, by after prismatic decomposition, fluorescence channel is incident upon on camera successively by the size of wavelength, forms light spectrum image-forming again by launching fiber.The advantage of the application is：Be capable of achieving once whole channel fluorescence collections is achieved without switching optical filter, switching when saving multi-channel detection between optical filtering.The edge effect of the Mechanical Moving and CCD drainage patterns between different sample wells is avoided by fibre system.It is thus possible to realize short time, efficient detection.
Description
Technical field
The present invention relates to the fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument.
Background technology
In molecule life field, polymerase chain reaction technology (Polymerase Chain Reaction, abbreviation PCR)
It is conventional experimental technique, its general principle, similar to the natural reproduction process of DNA, is that vitro enzyme promotes synthesis specific DNA piece
A kind of method of section, its specificity depends on the Oligonucleolide primers complementary with target sequence two ends.PCR it is basic by deformation, annealing,
Extend three fundamental reaction steps to constitute, have the advantages that high specificity, sensitivity are high, easy to operate, time saving, cannot be only used for
The basic research such as Gene Isolation, clone and nucleic acid sequence analysis, and the diagnosis of clinical disease is applied to more and more widely.It is real
The instrument of existing Real-Time Fluorescent Quantitative PCR Technique is referred to as real-time fluorescence quantitative PCR instrument, and it is substantially exactly a temperature control device, energy
Denaturation temperature, renaturation temperature, elongating temperature are accurately controlled.General principle is：Illuminator sends exciting light, by optics
Component reaches the reaction tube with sample, and sample sends fluorescence after being excited.The fluorescence being inspired is arrived by optical module again
Up to detector.Optical filtering is a part of optical module, can pass through the light of specific wavelength, and product is distinguished so as to reach
Purpose.By the fluorescence of optical filtering by being captured by detector after optical module, the fluorescence after capture is turned by a series of photoelectricity
After changing, input computer carries out calculating analysis.The common detector of fluorescent PCR has camera, PMT (photomultiplier), photoelectricity
Diode etc..
Traditional fluorescence detecting system, exciting light is sent by light source, and exciting light is swashed by after incident light optical filtering
Hair, the fluorescence after exciting is again by reaching detector (such as photodiode, camera) after detection light optical filtering.But no matter use
Photodiode or camera, as detector, are all the fluorescence of the wavelength needed for being obtained using optical filtering.Multichannel is glimmering
Light detection needs the fluorescence of various optical filterings, different wave length to select or rotate optical filtering by filter wheel；Or various optical filterings
Mirror is arranged on a shaven head, is moved back and forth by shaven head, is hole-specifically scanned.Both optical filtering modes, are required for mechanical movement to fill
Put, cannot otherwise obtain required fluorescence.And the introducing of Mechanical Moving structure (device) so that instrument become it is complicated, huge,
Cost also remains high；More importantly motion causes that whole instrument is in a dynamic detecting system, easily triggers setting
Standby failure, is that subsequent maintenance brings huge cost and burden.In addition, the optical system of common PCR instrument is normally at instrument top
Portion.Sample of the excitation source in projecting sample cell after incident optical filtering, the fluorescence that sample is excited leads to again
It is projected directly on the photoelectric detectors such as photosensitive receiving tube or CCD after crossing detection light optical filtering colour filter, so as to realize to be measured
The target of sample amounts detection.Exciting light is irradiated to sample cell and excites fluorescence and realize detection, this side from sample base top
Method is especially high to the cap quality requirement of example reaction pipe and agent plate, it is necessary to have high-transmittance, and is particularly easy to generation
Light path is chaotic.
The content of the invention
The subject matter that the application is solved is to provide the fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument,
To solve the technical problem of chaotic light path, scattering, light intensity exhaustion.
In order to solve the above-mentioned technical problem, the invention discloses a kind of fluorescence detection method of real-time fluorescence quantitative PCR instrument,
It is characterised in that it includes：LED light source sends light；The light incidence optical filtering forms the exciting light of blueness after filtering；It is described to excite
Light enters incident optical；The light that incident optical sends is projected directly at the sample in sample cell；Sample is excited
Fluorescence again by launching fiber, by after prismatic decomposition, being incident upon on camera successively by the size of wavelength.
Invention achieves following effect：
Using light emitting diode as light source, the light that light source sends is formed after the blue incidence optical filterings of 510nm filter
Exciting light, projects the sample in sample cell after being conducted through incident optical, sample is excited, and sends fluorescence.Fluorescence leads to again
Cross launching fiber to conduct to prism, because prism there are different refractive indexes to the light of different wave length, by after the refraction of prism, pressing
The light spectrum image-forming being divided into according to the size of wavelength is on camera.Computer calculates the intensity of the light of needs according to spectrum.Pass through
Prismatic decomposition and optical fiber light-guiding, be capable of achieving once whole channel fluorescence collections is achieved without switching optical filter, saves many
Switching during Air conduct measurement between optical filtering.The Mechanical Moving and CCD avoided by fibre system between different sample wells are adopted
The edge effect of integrated mode.It is thus possible to realize short time, efficient detection.
Brief description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes a part of the invention, this hair
Bright schematic description and description does not constitute inappropriate limitation of the present invention for explaining the present invention.In the accompanying drawings：
Fig. 1 is the flow chart of the fluorescence detection method of real-time fluorescence quantitative PCR instrument of the invention.
Fig. 2 is the structural representation of the fluorescence detecting system of real-time fluorescence quantitative PCR instrument of the invention
Specific embodiment
Some vocabulary have such as been used to censure specific components in the middle of specification and claim.Those skilled in the art should
It is understood that hardware manufacturer may call same component with different nouns.This specification and claims are not with name
The difference of title is used as distinguishing the mode of component, but the difference with component functionally is used as the criterion distinguished.Such as logical
The "comprising" of piece specification and claim mentioned in is an open language, therefore should be construed to " include but do not limit
In "." substantially " refer to that in receivable error range, those skilled in the art can solve described in the range of certain error
Technical problem, basically reaches the technique effect.Additionally, " coupling " one word herein comprising it is any directly and indirectly electric property coupling
Means.Therefore, if a first device is coupled to a second device described in text, representing the first device can direct electrical coupling
The second device is connected to, or the second device is electrically coupled to indirectly by other devices or coupling means.Specification
Subsequent descriptions be implement the application better embodiment, so it is described description be for the purpose of the rule for illustrating the application,
It is not limited to scope of the present application.The protection domain of the application ought be defined depending on the appended claims person of defining.
The application is described in further detail below in conjunction with accompanying drawing, but not as the restriction to the application.Hereafter in order to
Narration is convenient, hereinafter alleged " left side " " right side " " on " D score etc. is left and right in itself with accompanying drawing, upper and lower etc., and direction is consistent, " preceding
End ", " rear end " or " end " etc. are front or rear relative to accompanying drawing." first ", " second " hereinafter etc. is to be subject to area in description
Divide, not other particular meanings.
Embodiment one：
Refer to Fig. 1, a kind of fluorescence detection method of real-time fluorescence quantitative PCR instrument of the invention, including：LED light source 1 is sent out
Light extraction；The light incidence optical filtering 2 forms exciting light after filtering；The exciting light enters incident optical；What incident optical sent
Light is projected directly at the sample 3 in sample cell；The fluorescence that sample is excited passes through launching fiber again, by prism
After 4 light splitting, fluorescence channel is incident upon on camera 5 successively by the size of wavelength, forms light spectrum image-forming.
Preferably, the wavelength of the fluorescence is 510-750nm.Specifically, the inspection of 120 fluorescence channels can be realized in theory
Survey, greatly break through and improve the limitation of the most 4-5 bars fluorescence channels of existing quantitative real time PCR Instrument.By prismatic decomposition, can be real
What now disposable whole channel fluorescence was gathered is achieved without switching optical filtering, optical filtering switching when saving multi-channel detection
Time.
Preferably, the number of the fluorescence channel is 120.
Preferably, also include：Computer is calculated the light spectrum image-forming, the step of to obtain light intensity value.
Preferably, the optical filtering 2 is 500nm blue light optical filterings.
Preferably, including：LED light source 1, for sending light；Optical filtering 2, forms for receiving after the light incidence filters
Exciting light；Incident optical, for receiving the exciting light；Sample 3, the light for directly receiving incident optical projection；
Launching fiber, for receiving the fluorescence that sample is excited, by after the light splitting of prism 4, fluorescence channel press the size of wavelength according to
It is secondary to be incident upon on camera 5, form light spectrum image-forming.
Preferably, the wavelength of the fluorescence is 510-750nm.
Preferably, the number of the fluorescence channel is 120.
Preferably, also include：Computer, for the light spectrum image-forming to be calculated, the step of to obtain light intensity value.
Preferably, the optical filtering 2 is 500nm blue light optical filterings.
Because method part has been described in detail to the embodiment of the present application, the system to being related in embodiment here
Expansion with method corresponding part describes to omit, and repeats no more.Method is referred to for the description of particular content in system to implement
The content of example is no longer specific here to limit.
Compared with prior art, a kind of fluorescence detection method of real-time fluorescence quantitative PCR instrument of the present invention, reaches
Following effect：
Using light emitting diode as light source, the light that light source sends is formed after the blue incidence optical filterings of 510nm filter
Exciting light, projects the sample in sample cell after being conducted through incident optical, sample is excited, and sends fluorescence.Fluorescence leads to again
Cross launching fiber to conduct to prism, because prism there are different refractive indexes to the light of different wave length, by after the refraction of prism, pressing
The light spectrum image-forming being divided into according to the size of wavelength is on camera.Computer calculates the intensity of the light of needs according to spectrum.Pass through
Prismatic decomposition and optical fiber light-guiding, be capable of achieving once whole channel fluorescence collections is achieved without switching optical filter, saves many
Switching during Air conduct measurement between optical filtering.The Mechanical Moving and CCD avoided by fibre system between different sample wells are adopted
The edge effect of integrated mode.It is thus possible to realize short time, efficient detection.
Described above has shown and described some preferred embodiments of the application, but as previously described, it should be understood that the application
Be not limited to form disclosed herein, be not to be taken as the exclusion to other embodiment, and can be used for various other combinations,
Modification and environment, and can be in application contemplated scope described herein, by above-mentioned teaching or the technology or knowledge of association area
It is modified.And the change and change that those skilled in the art are carried out do not depart from spirit and scope, then all should be in this Shen
Please be in the protection domain of appended claims.
Claims (10)
1. a kind of fluorescence detection method of real-time fluorescence quantitative PCR instrument, it is characterised in that including：
LED light source sends light；
The light incidence optical filtering forms exciting light after filtering；
The exciting light enters incident optical；
The light that incident optical sends is projected directly at the sample in sample cell；
The fluorescence that sample is excited again by launching fiber, by after prismatic decomposition, fluorescence channel press the size of wavelength according to
It is secondary to be incident upon on camera, form light spectrum image-forming.
2. the fluorescence detection method of real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that the fluorescence
Wavelength be 510-750nm.
3. the fluorescence detection method of real-time fluorescence quantitative PCR instrument according to claim 2, it is characterised in that the fluorescence
The number of passage is 120.
4. the fluorescence detection method of real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that also include：Meter
Calculation machine is calculated the light spectrum image-forming, the step of to obtain light intensity value.
5. the fluorescence detection method of real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that the optical filtering
Mirror is 500nm blue light optical filterings.
6. a kind of fluorescence detecting system of real-time fluorescence quantitative PCR instrument, it is characterised in that including：
LED light source, for sending light；
Optical filtering, exciting light is formed for receiving after the light incidence filters；
Incident optical, for receiving the exciting light；
Sample, the light for directly receiving incident optical projection；
Launching fiber, for receiving the fluorescence that sample is excited, by after prismatic decomposition, fluorescence channel presses the size of wavelength
It is incident upon on camera successively, forms light spectrum image-forming.
7. the fluorescence detecting system of real-time fluorescence quantitative PCR instrument according to claim 6, it is characterised in that the fluorescence
Wavelength be 510-750nm.
8. the fluorescence detecting system of real-time fluorescence quantitative PCR instrument according to claim 7, it is characterised in that the fluorescence
The number of passage is 120.
9. the fluorescence detecting system of real-time fluorescence quantitative PCR instrument according to claim 8, it is characterised in that also include：Meter
Calculation machine, for the light spectrum image-forming to be calculated, the step of to obtain light intensity value.
10. the fluorescence detecting system of real-time fluorescence quantitative PCR instrument according to claim 6, it is characterised in that the optical filtering
Mirror is 500nm blue light optical filterings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611052722.7A CN106770086A (en) | 2016-11-24 | 2016-11-24 | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611052722.7A CN106770086A (en) | 2016-11-24 | 2016-11-24 | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106770086A true CN106770086A (en) | 2017-05-31 |
Family
ID=58912941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611052722.7A Pending CN106770086A (en) | 2016-11-24 | 2016-11-24 | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106770086A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008854A (en) * | 2021-03-03 | 2021-06-22 | 艾普拜生物科技(苏州)有限公司 | Optical fiber array imaging detection device |
| CN114480111A (en) * | 2022-02-15 | 2022-05-13 | 深圳阿斯克医疗有限公司 | Real-time fluorescence quantitative PCR instrument |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101300478A (en) * | 2005-11-03 | 2008-11-05 | 霍夫曼-拉罗奇有限公司 | Analytical multi-spectral optical detection system |
| CN102341694A (en) * | 2009-01-08 | 2012-02-01 | It-Is国际有限公司 | Optical system for chemical and/or biochemical reactions |
| CN105866090A (en) * | 2016-06-03 | 2016-08-17 | 苏州百源基因技术有限公司 | Ultraviolet visible light fluorescence detection system |
| CN105973855A (en) * | 2016-06-03 | 2016-09-28 | 苏州百源基因技术有限公司 | Infrared visible light fluorescence detection system |
| CN106030288A (en) * | 2014-04-03 | 2016-10-12 | 株式会社日立高新技术 | Fluorometric analyzer |
-
2016
- 2016-11-24 CN CN201611052722.7A patent/CN106770086A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101300478A (en) * | 2005-11-03 | 2008-11-05 | 霍夫曼-拉罗奇有限公司 | Analytical multi-spectral optical detection system |
| CN102341694A (en) * | 2009-01-08 | 2012-02-01 | It-Is国际有限公司 | Optical system for chemical and/or biochemical reactions |
| CN106030288A (en) * | 2014-04-03 | 2016-10-12 | 株式会社日立高新技术 | Fluorometric analyzer |
| CN105866090A (en) * | 2016-06-03 | 2016-08-17 | 苏州百源基因技术有限公司 | Ultraviolet visible light fluorescence detection system |
| CN105973855A (en) * | 2016-06-03 | 2016-09-28 | 苏州百源基因技术有限公司 | Infrared visible light fluorescence detection system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008854A (en) * | 2021-03-03 | 2021-06-22 | 艾普拜生物科技(苏州)有限公司 | Optical fiber array imaging detection device |
| CN114480111A (en) * | 2022-02-15 | 2022-05-13 | 深圳阿斯克医疗有限公司 | Real-time fluorescence quantitative PCR instrument |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Benavides et al. | Multispectral digital colposcopy for in vivo detection of cervical cancer | |
| WO2016124083A1 (en) | Superminiature multi-channel real-time fluorescence spectrometer | |
| EP1830174B1 (en) | Multi-channel fluorescence sample analyzer | |
| US20100032582A1 (en) | Fluorescence detection system and method | |
| CN105738331A (en) | Two-laser induced fluorescence multi-color detector used for single-cell electrophoretic chip | |
| CN107209102B (en) | Optical detection system and method of use | |
| CN102062731A (en) | Fiber spectroscopic probe mountable on a microscope | |
| CN102243165B (en) | Photonic crystal coded microsphere biochip detection device | |
| CN109406479A (en) | A kind of the real-time fluorescence detection device and its operating method of microreactor | |
| WO2017173896A1 (en) | Flow cytometry detection apparatus and method | |
| CN106770086A (en) | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument | |
| CN106770109A (en) | A kind of bioluminescence detecting system | |
| EP4324934A1 (en) | Fluorescence quantitative pcr processing method and system | |
| CN211453365U (en) | Coaxial optical fiber fluorescent gene detection device | |
| CN209296581U (en) | A kind of real-time fluorescence detection device of microreactor | |
| CN211374514U (en) | Light-guide rod light-transmission lens-free illumination imaging device and micro-fluidic chip detection system | |
| CN109929747B (en) | Digital PCR detection device and detection method | |
| US20230221178A1 (en) | Apparatus and a method for fluorescence imaging | |
| EP3179231B1 (en) | Optoelectronic device for use in the colorimetric analysis of a sample fluid, apparatus and method for colorimetric analysis of a sample fluid | |
| CN113189065B (en) | Optical detection method | |
| CN105241855B (en) | Microchannel electrophoresis quantitative analysis device and method | |
| CN207540961U (en) | Enhanced sensitivity collection device is coupled suitable for the multichannel optical signal of spectrum analysis | |
| CN202693475U (en) | Ultraviolet spot fluorescent scanner | |
| US11892389B2 (en) | Flow cytometer including light collection modules, and methods of using the same | |
| CN209927718U (en) | Remote measurement device based on fluorescence technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |
|
| RJ01 | Rejection of invention patent application after publication |