CN106755359A - A kind of screening or the method and its dedicated kit of assisting sifting difference plant height wheat - Google Patents
A kind of screening or the method and its dedicated kit of assisting sifting difference plant height wheat Download PDFInfo
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Abstract
The invention discloses a kind of screening or the method and its dedicated kit of assisting sifting difference plant height wheat.Screening provided by the present invention or the method for assisting sifting difference plant height wheat, comprise the following steps：Detect that the genotype that wheat to be measured is based on TaSPL21 6A genes is genotype HapI, genotype HapII or genotype HapIII, the plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII.It is demonstrated experimentally that method provided by the present invention is based on the genotype of TaSPL21 6A genes by detecting wheat to be measured, Plant Height in Wheat proterties can be screened, there is important application value in Wheat Molecular Breeding.
Description
Technical field
The present invention relates to biological technical field, more particularly to it is a kind of screen or assisting sifting difference plant height wheat method and
Its dedicated kit.
Background technology
Wheat is one of cereal crops main in the world, accounts for the 17% of world food crops planting area, has supported generation
The population on boundary 40%, 20% food energy and protein is provided for the mankind, and the population of whole world 35-40% is main with it
Food source.Therefore, wheat yield is directly connected to the grain security in the world.At present, in the case of limited cultivated area,
The new variety of wheat that seed selection per unit area yield is protruded is to stabilize and increase the most economical effective approach of wheat yield.Wheat yield by it is various because
The influence of plain (such as spike number, grain number per spike and grain weight), the coordination of each factor is the key of improving yield of wheat.Plant height is that influence wheat is produced
One of key factor of amount, it shows as：Within the specific limits, with the increase of plant height, wheat yield also accordingly increases；But it is super
Go out certain limit, with the increase of plant height, wheat yield declines on the contrary, main cause for leaf-area coefficient reached optimum value,
Easily lodging, harvesting difficulty etc..Therefore, the excellent allelic variation of control Plant Height in Wheat correlated traits is excavated, and develops corresponding
The functional label of allelic variation has great importance to the molecular mark of High-Yield Wheat Cultivar.
Single nucleotide polymorphism (Single nucleotide polymorphism, SNP) refers in genomic level
By the polymorphism of the caused DNA sequence dna of single nucleotide acid variation.With the development of technology, SNP detection method is more and more, inspection
Survey expense is also more and more economical, makes it the molecular labeling of new generation after SSR.At present in Wheat volatiles research process
In, SNP marker has been applied to the structure of wheat genetic collection of illustrative plates, the positioning of wheat Main Agronomic Characters related gene and genome
The aspects such as association analysis.
Cleaved amplified polymorphic sequence marker (Cleaved amplified polymorphism sequence, CAPS)
DNA fragmentation mainly to PCR amplifications carries out restricted enzyme cutting analysis.Special primer is designed according to gene order, by specific PCR
Amplification is combined a kind of molecular marking technique for detecting nucleotide sequence polymorphism with two methods of restricted digestion.Its base
Present principles are to know that the DNA sequence dna in site designs a pair special PCR primers using oneself, then with the primer specific DNA amplification piece
Section, afterwards with the pcr amplification product obtained by specific restriction digest, separates finally by gel electrophoresis and examines
Survey digestion products.
The content of the invention
The technical problems to be solved by the invention be how to screen or assisting sifting difference plant height wheat.
In order to solve the above technical problems, present invention firstly provides screening or the method for assisting sifting difference plant height wheat.
Screening provided by the present invention or the method for assisting sifting difference plant height wheat, concretely method one, it may include
Following steps：Detect that the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI, genotype HapII or base
Because of type HapIII, the plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The wheat of genotype HapII
Plant height；
The wheat of the genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, based on T160C
The genotype of SNP site is that CC is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；The gene
The wheat of type HapII is the genotype based on T125C SNP sites for CC is homozygous, genotype based on T160C SNP sites
For TT is homozygous and genotype based on C169G SNP sites is GG homozygous wheat；The wheat of the genotype HapIII
Be the genotype based on T125C SNP sites for CC is homozygous, the genotype based on T160C SNP sites be that TT is homozygous and base
In the genotype of C169G SNP sites be the homozygous wheats of CC；
" the T125C SNP sites " the 125th nucleosides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles
Acid；" the T160C SNP sites " the 160th nucleotides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles；Institute
State " C169G SNP sites " for the sequence 9 in sequence table in Wheat volatiles the 169th nucleotides from 5 ' ends.
Screening provided by the present invention or the method for assisting sifting difference plant height wheat, concretely method two, it may include
Following steps：Detect that the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI, genotype HapII or base
Because of type HapIII, the plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The wheat of genotype HapII
Plant height；
The wheat of the genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, based on T160C
The genotype of SNP site is that CC is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；The gene
The wheat of type HapII is the genotype based on T125C SNP sites for CC is homozygous, genotype based on T160C SNP sites
For TT is homozygous and genotype based on C169G SNP sites is GG homozygous wheat；The wheat of the genotype HapIII
Be the genotype based on T125C SNP sites for CC is homozygous, the genotype based on T160C SNP sites be that TT is homozygous and base
In the genotype of C169G SNP sites be the homozygous wheats of CC；
" the T125C SNP sites " the 125th core from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles
Thuja acid；" the T160C SNP sites " the 160th nucleosides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles
Acid；" the C169G SNP sites " the 169th nucleotides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles.
Screening provided by the present invention or the method for assisting sifting difference plant height wheat, concretely method three, successively may be used
Comprise the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded by the primer pair constituted using primers F and primer R
Increase, obtain pcr amplification product；
(2) pcr amplification product is carried out into digestion with restriction enzyme BglI, obtains digestion products A；Will be described
Pcr amplification product carries out digestion with restriction enzyme BseDI or SecI, obtains digestion products B；
(3) judged as follows：
If the digestion products A is two DNA fragmentations and the digestion products B is a DNA fragmentation, wheat to be measured
Genotype based on TaSPL21-6A genes is genotype HapI；If the digestion products A is a DNA fragmentation and the enzyme
Product B is cut for two DNA fragmentations, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapII；If institute
Digestion products A is stated for a DNA fragmentation and the digestion products B is a DNA fragmentation, then wheat to be measured is based on TaSPL21-6A
The genotype of gene is genotype HapIII；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The strain of the wheat of genotype HapII
It is high；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of congenerous；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of congenerous.
Screening provided by the present invention or the method for assisting sifting difference plant height wheat, concretely method four, successively may be used
Comprise the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded by the primer pair constituted using primers F and primer R
Increase, obtain pcr amplification product；
(2) pcr amplification product is carried out into digestion with restriction enzyme BglI, obtains digestion products A；Will be described
Pcr amplification product carries out digestion with restriction enzyme BseDI or SecI, obtains digestion products B；
(3) judged as follows：
If the DNA fragmentation in digestion products A with 176bp and tool in the DNA fragmentation and the digestion products B of 166bp
There is the DNA fragmentation of 342bp, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI；If the enzyme
Cutting product A, there is the DNA fragmentation and digestion products B of 342bp to have the DNA fragmentation of 165bp and the DNA fragmentation of 177bp, then to be measured
The genotype that wheat is based on TaSPL21-6A genes is genotype HapII；If the digestion products A has the DNA pieces of 342bp
Section and digestion products B has the DNA fragmentation of 342bp, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype
HapIII；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The strain of the wheat of genotype HapII
It is high；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of congenerous；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of congenerous.
Screening provided by the present invention or the method for assisting sifting difference plant height wheat, concretely method five, successively may be used
Comprise the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded by the primer pair constituted using primers F and primer R
Increase, obtain pcr amplification product；
(2) pcr amplification product is sequenced；
(3) judged as follows：
If the sequence 1 in the nucleotide sequence of the pcr amplification product such as sequence table is the 1st to 342 from 5' ends
Shown, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI；If the core of the pcr amplification product
As shown in the sequence 2 the 1st to 342 from 5' ends in sequence table, then wheat to be measured is based on TaSPL21-6A bases to nucleotide sequence
The genotype of cause is genotype HapII；If sequence 3 in the nucleotide sequence of the pcr amplification product such as sequence table is from 5'
End is risen shown in the 1st to 342, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapIII；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The strain of the wheat of genotype HapII
It is high；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of congenerous；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of congenerous.
It is described in any of the above-described methods described>Concretely statistically>.
In order to solve the above technical problems, present invention also offers a kind of kit identified or aid in identification Plant Height in Wheat.
Identification provided by the present invention or the kit of auxiliary identification Plant Height in Wheat, including detect that wheat to be measured is based on
The genotype of TaSPL21-6A genes is the material of genotype HapI, genotype HapII or genotype HapIII；
The genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, based on T160C SNP sites
Genotype for CC is homozygous and genotype based on C169G SNP sites is CC homozygous TaSPL21-6A genes；It is described
Genotype HapII is the genotype based on T125C SNP sites for CC is homozygous, genotype based on T160C SNP sites is
TT is homozygous and genotype based on C169G SNP sites is GG homozygous TaSPL21-6A genes；The genotype
HapIII is the genotype based on T125C SNP sites for CC is homozygous, the genotype based on T160C SNP sites is TT homozygosis
Type and the genotype based on C169G SNP sites are the homozygous TaSPL21-6A genes of CC；
" the T125C SNP sites " be in Wheat volatiles the sequence 9 in sequence table or the sequence 10 in sequence table from
Play the 125th nucleotides in 5 ' ends；" the T160C SNP sites " is the sequence 9 or sequence in sequence table in Wheat volatiles
Sequence 10 in table the 160th nucleotides from 5 ' ends；" the C169G SNP sites " is in sequence table in Wheat volatiles
Sequence 9 or sequence table in sequence 10 from 5 ' ends the 169th nucleotides.
In mentioned reagent box, described " genotype that detection wheat to be measured is based on TaSPL21-6A genes is genotype HapI,
The material of genotype HapII or genotype HapIII " can be the primer pair that primers F and primer R are constituted；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of congenerous；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into phase by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of congenerous.
Mentioned reagent box, may also include restriction enzyme BglI and/or restriction enzyme BseDI and/or restricted
Restriction endonuclease SecI.
In order to solve the above technical problems, present invention also offers molecular labeling first and molecular labeling second.
Molecular labeling first provided by the present invention can be as shown in the sequence 10 of sequence table.
Molecular labeling second provided by the present invention can be as shown in the sequence 9 of sequence table.
Or (z2) or (z3) or (z4) or (z5) falls within protection scope of the present invention (z1)；
(z1) any of the above-described kit or, the molecular labeling first or, the molecular labeling second screening or it is auxiliary
The application helped in the different plant height wheats of screening；
(z2) any of the above-described kit or, the molecular labeling first or, the molecular labeling second prepare screen
Or the application in the product of assisting sifting difference plant height wheat；
(z3) any of the above-described kit or, the molecular labeling first or, the molecular labeling second identification wheat
Application in the genotype of TaSPL21-6A genes；
(z4) any of the above-described kit or, the molecular labeling first or, the molecular labeling second prepare identify
Application in the product of the genotype of wheat TaSPL21-6A genes；
(z5) any of the above-described kit or, the molecular labeling first or, the molecular labeling second is in wheat breeding
In application.
In above-mentioned application, when the genotype of the T125C SNP sites of the molecular labeling first is for TT is homozygous, T160C
The genotype of SNP site is that CC is homozygous and genotype of C169G SNP sites is for CC is homozygous, then be judged to genotype HapI
Wheat；When the genotype of the T125C SNP sites of the molecular labeling first is for CC is homozygous, T160C SNP sites gene
Type is that TT is homozygous and genotype of C169G SNP sites is for GG is homozygous, then be judged to the wheat of genotype HapI I；Work as institute
The genotype of T125C SNP sites of molecular labeling first is stated for CC is homozygous, T160C SNP sites genotype is that TT is homozygous
And the genotype of C169G SNP sites is that CC is homozygous, then be judged to the wheat of genotype HapIII；Described " T125C SNP
Point " the 125th nucleotides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles；" the T160C SNP sites "
For the sequence 10 in sequence table in Wheat volatiles from 5 ' ends the 160th nucleotides；" the C169G SNP sites " is small
Sequence 10 in wheat genome in sequence table the 169th nucleotides from 5 ' ends；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The strain of the wheat of genotype HapII
It is high.
In above-mentioned application, when the genotype of the T125C SNP sites of the molecular labeling second is for TT is homozygous, T160C
The genotype of SNP site is that CC is homozygous and genotype of C169G SNP sites is for CC is homozygous, then be judged to genotype HapI
Wheat；When the genotype of the T125C SNP sites of the molecular labeling second is for CC is homozygous, T160C SNP sites gene
Type is that TT is homozygous and genotype of C169G SNP sites is for GG is homozygous, then be judged to the wheat of genotype HapII；Work as institute
The genotype of T125C SNP sites of molecular labeling second is stated for CC is homozygous, T160C SNP sites genotype is that TT is homozygous
And the genotype of C169G SNP sites is that CC is homozygous, then be judged to the wheat of genotype HapIII；Described " T125C SNP
Point " the 125th nucleotides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles；" the T160C SNP sites " is
Sequence 9 in Wheat volatiles in sequence table the 160th nucleotides from 5 ' ends；" the C169G SNP sites " is wheat
Sequence 9 in genome in sequence table the 169th nucleotides from 5 ' ends；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The strain of the wheat of genotype HapII
It is high.
It is described in above-mentioned application>Concretely statistically>.
It is demonstrated experimentally that method provided by the present invention is based on the gene of TaSPL21-6A genes by detecting wheat to be measured
Type, can screen or assisting sifting Plant Height in Wheat proterties, have important application value in Wheat Molecular Breeding.
Brief description of the drawings
Fig. 1 is nucleotides of three genotype at T125C SNP, T160C SNP and C169G SNP.
Fig. 2 is the electrophoresis result in the step 2 of embodiment 2 3).
Fig. 3 is the electrophoresis result in the step 2 of embodiment 2 4).
Fig. 4 is the association analysis of the wheat with plant height of the different lower three kinds of genotype of planting conditions.E1 is Bei Jingchang in 2009
Flat nonirrigated farmland, E2 is Changping County, Beijing water ground in 2009, and E3 is Shunyi nonirrigated farmland of Beijing in 2009, and E4 is the Shunyi of Beijing in 2009 water ground,
E5 is Shunyi nonirrigated farmland of Beijing in 2009 and carries out heat stress, and E6 is the Shunyi of Beijing in 2009 water ground and carries out heat stress, and E7 is
Changping County, Beijing nonirrigated farmland in 2010, E8 is Changping County, Beijing water ground in 2010, and E9 is Shunyi nonirrigated farmland of Beijing in 2010, and E10 is 2010
Beijing Shunyi water ground, E11 is Shunyi nonirrigated farmland of Beijing in 2010 and carries out heat stress, and E12 goes forward side by side for the Shunyi of Beijing in 2010 water
Row heat stress, E13 is Shunyi nonirrigated farmland of Beijing in 2011, and E14 is the Shunyi of Beijing in 2011 water ground, and E15 is the Shunyi of Beijing in 2011
Nonirrigated farmland simultaneously carries out heat stress, and E16 is the Shunyi of Beijing in 2011 water ground and carries out heat stress, and E17 is Changping County, Beijing nonirrigated farmland in 2012,
E18 is Changping County, Beijing water ground in 2012, and E19 is Shunyi nonirrigated farmland of Beijing in 2012, and E20 is the Shunyi of Beijing in 2012 water ground；* it is P
＜ 0.5, * * are P ＜ 0.01, and * * * are P ＜ 0.001.
Specific embodiment
The present invention is further described in detail with reference to specific embodiment, the embodiment for being given is only for explaining
The bright present invention, rather than in order to limit the scope of the present invention.
Experimental technique in following embodiments, unless otherwise specified, is conventional method.
Material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.
Wheat lines used are all from national germplasm resource bank (network address are in following embodiments：http://
Www.cgris.net/), the public can obtain from national germplasm resource bank.
5 × PCR buffer and TransStart Fast Pfu DNA Polymerase are the full formula gold biology skill in Beijing
Art Co., Ltd product, catalog number is AP221.DNTPs is Roche Products, and product article No. is #316K5S.
Embodiment 1, the acquisition of wheat TaSPL21-6A gene polymorphism sites and the acquisition of haplotype
First, the acquisition of wheat TaSPL21-6A gene polymorphism sites
Respectively extract table 1 shown in 37 genomic DNAs of wheat breed and using it as template, with artificial synthesized
5'-GGATAGTGCAGTGAAGCGGT-3' and 5'-CCGTGTAAGAGGGTAACACTGA-3' is primer, enters performing PCR amplification, is obtained
To pcr amplification product.Each pcr amplification product is sequenced, is then compared.
Table 1
| Numbering | Wheat breed | Numbering | Wheat breed |
| 1 | TAM-110 | 20 | Henan wheat 48 |
| 2 | 124-1 in peace 85 | 21 | Huaihe River wheat 18 |
| 3 | Single R8108 | 22 | Bumper harvests |
| 4 | The former winter 847 | 23 | Shandong wheat 14 |
| 5 | Beijing 10 | 24 | The excellent wheat of mountain agriculture No. 2 |
| 6 | Capital core 8922 | 25 | Long 6359 |
| 7 | Zhongyou9507 | 26 | Shanxi wheat 16 |
| 8 | Deep blue wheat 6005 | 27 | Shanxi wheat 25 |
| 9 | Weighing apparatus wheat No. 2 | 28 | Shanxi wheat 39 |
| 10 | Handan 6050 | 29 | Drought selects No. 10 |
| 11 | Weighing apparatus 7228 | 30 | Shanxi wheat 51 |
| 12 | Weighing apparatus sees 35 | 31 | Linfeng 615 |
| 13 | Stone 4185 | 32 | Taiyuan 633 |
| 14 | Stone wheat No. 12 | 33 | Fortune drought 20410 |
| 15 | Hundred agricultures 3217 | 34 | PANDAS |
| 16 | Lay down and open up No. one | 35 | Opata 85 |
| 17 | Yanzhan4110 | 36 | W7984 |
| 18 | Henan wheat No. 29 | 37 | Interior township 188 |
| 19 | Henan wheat 38 |
Comparison result shows that 3 SNP sites of discovery, are respectively designated as in the TaSPL21-6A genes of wheat
T125CSNP, T160CSNP and C169G SNP.T125CSNP is located at the 125th from 5 ' ends of sequence 1 in sequence table,
T160CSNP is located at the 160th from 5 ' ends ing of sequence 1 in sequence table, and sequence 1 is last from 5 ' during T169CSNP is located at sequence table
The 169th for having held.The essential information of 3 SNP sites is shown in Table 2 (because genomic DNA is single-stranded by two of reverse complemental
DNA molecular constitutes double chain DNA molecule, therefore the general DNA molecular by coded protein, that is, with initiation codon to end
Only the DNA molecular of codon, is named as sense dna molecule；DNA molecular with the reverse complemental of sense dna molecule is named as
Anti-sense DNA molecules.It is intended that the genotype of the allele at T125C SNP, T160C SNP and C169G SNP is equal
It is the genotype of sense dna).
Table 2
| SNP site title | The genotype of allele |
| T125C SNP | TT is homozygous, CC is homozygous |
| T160C SNP | TT is homozygous, CC is homozygous |
| C169G SNP | CC is homozygous, GG is homozygous |
2nd, the acquisition of the haplotype of wheat TaSPL21-6A genes
Comparison result according to step one finds, in wheat natural variation colony, T125C SNP, T160C SNP and
C169G SNP only exist three kinds of haplotypes, therefore, wheat is divided into by three kinds of genotype according to wheat TaSPL21-6A genes：
TaSPL21-6A-HapI (hereinafter referred to as genotype HapI), TaSPL21-6A-HapII (hereinafter referred to as genotype HapII) and
TaSPL21-6A-HapIII (hereinafter referred to as genotype HapIII).Each genotype in T125C SNP, T160C SNP and
Nucleotides at C169G SNP refers to Fig. 1.Equipotential of each genotype at T125C SNP, T160C SNP and C169G SNP
The genotype of gene is shown in Table 3.Sequence in the nucleotide sequence of the TaSPL21-6A genes of the wheat of genotype HapI such as sequence table
Shown in row 1.The nucleotide sequence of the TaSPL21-6A genes of the wheat of genotype HapII is as shown in the sequence 2 in sequence table.Base
Because the wheat of type HapIII TaSPL21-6A genes nucleotide sequence as shown in the sequence 3 in sequence table.
Table 3
Embodiment 2, the genotype based on wheat TaSPL21-6A genes carry out building for TaSPL21-6A methods of genotyping
It is vertical
First, the preparation of special primer pair
Being designed for amplification includes the special primer of the target sequence of T125C SNP, T160C SNP and C169G SNP to such as
Under：
Primers F：Sequence 4 in 5'-GGATAGTGCAGTGAAGCGGT-3'(sequence tables)；
Primer R：Sequence 5 in 5'-TGGCATCTCTTGGGCTCTT-3'(sequence tables)；
2nd, the genotype based on wheat TaSPL21-6A genes carries out the foundation of TaSPL21-6A methods of genotyping
1st, method first
1) genomic DNA of wheat to be measured is extracted；
2) with step 1) genomic DNA be template, the primer pair constituted with primers F described in step one and primer R is carried out
PCR is expanded, and obtains pcr amplification product.
Pcr amplification reaction system (15 μ L)：ddH2The μ L of O 8.0, the μ L of 5 × PCR buffer 3.0, primers F (5 μm of ol/L)
With primer R (5 μm of ol/L) each 0.6 μ L, dNTPs (2.5 μm of ol/L) 0.4 μ L, TransStart Fast Pfu DNA
Polymerase0.3 μ L (the Pfu DNA Polymerase of Fast containing TransStart 0.75U), genomic DNA (20ng/ μ
L)2.1μL。
Pcr amplification reaction condition is:95℃5min；95 DEG C of 1min, 59 DEG C of 45s, 72 DEG C of 1min, 35 circulations；72℃
10min；4 DEG C of preservations.
3) by step 2) pcr amplification product that obtains is digested with restriction enzyme BglI, obtains digestion product
Thing A, then enters row agarose gel electrophoresis detection.
4) by step 2) pcr amplification product that obtains is digested with restriction enzyme BseDI or SecI, obtained
Digestion products B, then enters row agarose gel electrophoresis detection.
If digestion products A shows banding pattern A1 (two bands of display, respectively 176bp and 166bp) and digestion products B shows
Banding pattern B2 (one band of display, be 342bp), then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI；
If digestion products A displays banding pattern A2 (one band of display, be 342bp) and digestion products B display banding patterns B1 (two bands of display,
Respectively 165bp and 177bp), then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapII；If enzyme
Cut product A display banding patterns A2 (one band of display, be 342bp) and digestion products B display banding patterns B2 (shows a band, is
342bp), then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapIII.
2nd, method second
1) genomic DNA of wheat to be measured is extracted；
2) with step 1) genomic DNA be template, the primer pair constituted with primers F described in step one and primer R is carried out
PCR is expanded, and obtains pcr amplification product.
Pcr amplification reaction system (15 μ L)：ddH2The μ L of O 8.0, the μ L of 5 × PCR buffer 3.0, primers F (5 μm of ol/L)
With primer R (5 μm of ol/L) each 0.6 μ L, dNTPs (2.5 μm of ol/L) 0.4 μ L, TransStart Fast Pfu DNA
Polymerase0.3 μ L (the Pfu DNA Polymerase of Fast containing TransStart 0.75U), genomic DNA (20ng/ μ
L)2.1μL。
Pcr amplification reaction condition is:95℃5min；95 DEG C of 1min, 59 DEG C of 45s, 72 DEG C of 1min, 35 circulations；72℃
10min；4 DEG C of preservations.
3) by step 2) pcr amplification product that obtains is sequenced.
If the nucleotide sequence of pcr amplification product is as shown in the sequence 1 the 1st to 342 from 5' ends in sequence table,
The genotype that then wheat to be measured is based on TaSPL21-6A genes is genotype HapI；If the nucleotide sequence of pcr amplification product
As shown in the sequence 2 the 1st to 342 from 5' ends in sequence table, then wheat to be measured is based on the gene of TaSPL21-6A genes
Type is genotype HapII；If the sequence 3 in the nucleotide sequence of pcr amplification product such as sequence table from 5' ends the 1st to
Shown in 342, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapIII.
The association analysis of embodiment 3, the genotype and Plant Height in Wheat of wheat TaSPL21-6A genes
Wheat to be measured is 262 wheat breeds (being hexaploid wheat), and specific wheat breed title refers in table 4 the
2 row.
First, the Genotyping of wheat to be measured
Genotyping is carried out to each wheat to be measured using the method first in the step 2 of embodiment 2.
The part of test results of restriction enzyme BglI digestions is used to see that (M is DNA Marker to Fig. 2, and C is banding pattern A1, T
For banding pattern A2).
The part of test results of restriction enzyme BseDI or SecI digestion is used to see that (M is DNA Marker to Fig. 3, and C is
Banding pattern B2, G are banding pattern B1)
Each wheat to be measured is based on the 3rd row during the genotypic results of the genotype of wheat TaSPL21-6A genes are shown in Table 4.
The above results show that, based on T125C SNP, T160C SNP and C169G SNP, wheat to be measured is based on TaSPL21-
The genotype of 6A genes is genotype HapI, genotype HapII or genotype HapIII.Because wheat breed is cultigen,
Therefore be generally defaulted as be high homogenous vegetable material, so the gene of T125C SNP, T160C SNP and C169G SNP
Type is homozygous.
2nd, plant height proterties detection
Each wheat breed (being hexaploid wheat) to be measured is planted in Changping County, Beijing nonirrigated farmland (letter below in 2009 respectively
Claim E1), the ground of Changping County, Beijing water in 2009 (hereinafter referred to as E2), Shunyi nonirrigated farmland of Beijing in 2009 (hereinafter referred to as E3), north in 2009
Capital Shunyi water ground (hereinafter referred to as E4), Shunyi nonirrigated farmland of Beijing in 2009 simultaneously carry out heat stress (hereinafter referred to as E5), Beijing in 2009
Shunyi water ground simultaneously carries out heat stress (hereinafter referred to as E6), Changping County, Beijing nonirrigated farmland (hereinafter referred to as E7), Bei Jingchang in 2010 in 2010
Par ground (hereinafter referred to as E8), Shunyi nonirrigated farmland of Beijing in 2010 (hereinafter referred to as E9), the Shunyi of Beijing in 2010 water ground are (hereinafter referred to as
E10), Shunyi nonirrigated farmland of Beijing in 2010 and heat stress (hereinafter referred to as E11), the Shunyi of Beijing in 2010 water ground is carried out and the hot side of body is carried out
Compel (hereinafter referred to as E12), Shunyi nonirrigated farmland of Beijing in 2011 (hereinafter referred to as E13), the Shunyi of Beijing in 2011 water ground (hereinafter referred to as
E14), Shunyi nonirrigated farmland of Beijing in 2011 and heat stress (hereinafter referred to as E15), the Shunyi of Beijing in 2011 water ground is carried out and the hot side of body is carried out
Compel (hereinafter referred to as E16), Changping County, Beijing nonirrigated farmland (hereinafter referred to as E17), Changping County, Beijing water ground in 2012 in 2012 (hereinafter referred to as
E18), Shunyi nonirrigated farmland of Beijing in 2012 (hereinafter referred to as E19) or the Shunyi of Beijing in 2012 water ground (hereinafter referred to as E20), after maturation,
Statistics Plant Height in Wheat, the 4th to 8 arranges during part statistics is shown in Table 4.It should be pointed out that Changping County, Beijing nonirrigated farmland, Changping County, Beijing water
It is the Experimental Base of Institute of Crop Science, Chinese Academy of Agricultural Science ground, Beijing Shunyi nonirrigated farmland and Beijing Shunyi water.It is described
The whole growth period that nonirrigated farmland refers to wheat to be measured only has natural rainwater irrigation, and any moisture is not artificially irrigated.The water ground
The only not natural rainwater of whole growth period for referring to wheat to be measured is irrigated, and (that artificially irrigates is specific for also artificial irrigation moisture
Operate and be：Respectively before Wintering Period, heading stage, florescence and pustulation period irrigate, the water irrigated every time is 750m3/ hectare
Water ground).The heat stress is, when wheat breed to be measured is in florescence, to start plastic covering greenhouse, until wheat breed to be measured
It is ripe.Heat stress is carried out in nonirrigated farmland, the outer temperature-averaging of vinyl house is 33 DEG C, and temperature is 43 DEG C in vinyl house.Enter in water
Row heat stress, the outer temperature-averaging of vinyl house is 33 DEG C, and temperature is 41 DEG C in vinyl house.
3rd, the association analysis of the genotype and Plant Height in Wheat of wheat TaSPL21-6A genes
The average plant height of the wheat of the different lower three kinds of genotype of planting conditions is counted respectively, and genotype is closed with plant height
Connection analysis, the results are shown in Table 5 and Fig. 4.
Table 4
Note："-" is represented and does not obtain data.
Table 5
Note：P value are the significance of association analysis；PVE is the explanation rate of phenotypic variation.
Result shows that detection wheat to be measured is based on the genotype of TaSPL21-6A genes, " wheat of genotype HapI "
Plant height>The plant height of " wheat of genotype HapIII ">The plant height of " wheat of genotype HapII "；Described ">" for statistically
's>.
4th, it is sequenced
" wheat of genotype HapI ", " genotype are detected as to step one using the method second in the step 2 of embodiment 2
The wheat of HapII " and " wheat of genotype HapIII " are sequenced respectively.Sequencing result shows that " genotype HapI's is small
The nucleotide sequence of the pcr amplification product of wheat " as shown in the sequence 1 the 1st to 342 from 5' ends in sequence table, " gene
The nucleotide sequence of the pcr amplification product of the wheat of type HapII " is if the sequence 2 in sequence table is the 1st to 342 from 5' ends
Shown in position, the nucleotide sequence of the pcr amplification product of " wheat of genotype HapIII " is such as the sequence 3 in sequence table from 5' ends
Hold shown in the 1st to 342.It can be seen that, the testing result of method first and method second is completely the same.
The above results show, can screen or aid in by detecting that wheat to be measured is based on the genotype of TaSPL21-6A genes
Screening Plant Height in Wheat proterties, has important application value in Wheat Molecular Breeding.
<110>Institute of Crop Science, Chinese Academy of Agricultural Science
<120>A kind of screening or the method and its dedicated kit of assisting sifting difference plant height wheat
<160> 10
<170> PatentIn version 3.5
<210> 1
<211> 3463
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 1
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatctgcccc agatttgggc tagatataag agatgtcggc cagcctggcg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc catcattgcc tctctccttc 360
tccggcctcc acgacctttt tagcctgaag gcacgcacaa ccgttcctgc tgcacagaca 420
catactccgg cggtccggcc tctttctttc ttacccatca gtcaggccgc gcgtcaccac 480
ctttctcatc cgcgctcgga atagccggcc atgatgagca gcaggctaag cggcggcacg 540
atggcaccgg ttagcgacat ggccgacttc ggttgcgctc ccatgcagtc ctaccccaac 600
tttgagccgg ccggcatggt catgcccggg gaccggcagc cgcccttcca gcaccaccac 660
ctctacgaca gcctcgactt caacgccgct gcattctcgt tccaagaccc ggtcgcgctc 720
ttctccagcg gctcggcgtt cagtaaccag ctccagcagc cgttcctcca gacgcaggtc 780
accacgccga cgatggcgtc gtcgtcgctg ctgcaggcgc cgatgatgac ccttccgggt 840
atgctgacgt cttcctcggc gtcgccggtg gacgcatgca ccttcggtgg cggcggcagt 900
gctgggttcc tgaagcagga ggagggcggc cccttctcag acgtcggcgg tggggggagg 960
atcgggctga acctcggccg caggacatac ttttccccgg cggacgtgct ggccgtggac 1020
cgcctgctga tgcgctcccg cttcggcgga gccggcgcaa tgggcatgct ggggctgggg 1080
ctcggcgccg ccgcccacca ccaccagacc ccgcggtgcc aggctgaggg ctgcaaggcc 1140
gacctctccg ccgccaagca ctaccaccgc cgccacaagg tctgcgagta ccacgccaag 1200
gccaccacag tcgccgcctc tggcaagcag cagcgcttct gccaacaatg cagccggtat 1260
gtacgtttat cctctgccat gggtggatcg ttgatgcatg cgaagctttg ctagcagacg 1320
aaaaaatcag cagtagaaag gctgcgagct ttcgatctag tggcattgac gctagcagct 1380
atatgcactg atcatatcat tcatgtcaac atgtcatata tcagaaatgg cgcacatcag 1440
atcagatctt gttcctctga gcgtatcggc atctgcatcc ctttgtccaa tgtactacta 1500
gcacgcccat catggcattg cagtggagtt cgatcagata cctaaaccta gtctgtgtag 1560
atacatcata catggcgtgt attacttaca acggtagaca tgtatatacc caagatccgg 1620
ttgtgtgaat ataactggtt cttgtttggg tacgtttgtt gggctaggtt tcatgtgctc 1680
gctgagtttg acgaggccaa gaggagctgc cggaagcggc tcacggagca caaccggcgc 1740
cgccgaaagc ctgccggcgc gcagggcaag gattcgccgc cgccttccaa gaaggcagac 1800
gctagcatca ccagctcata caccggcgat cacaagagta agaccactcc tctgatacat 1860
gtatagactt aaacactgga tatatgctta ttattggctt ttttaaagct tgatcacttt 1920
aagaagttgt gttttgattt tcggccgcaa aatgtggatt tcggccgaat ttcggtcatc 1980
tcggcttgag gcgaaaagta tatgtaggcc gaaattactc aaatttggta aattttggtc 2040
aaatttaagt caaattgcag tcaaaatttg atcaaatttc agccgaaaat tttgaaaatg 2100
gccgaaattc ggccatctcg gcctagggcg aaaaaaagct caaaccgaaa atcaaaacac 2160
agccagtaag ctagctagct acctagaaac tcctgaaact ttgttagagc ttagtgggta 2220
atttgaacta aaagcatgac acttcttttg caacaaggga acatttcaat aacgtatacc 2280
aagtaaagaa cacaacaaaa acagtttgcc taattcttaa ttgtccgaaa atttagtaaa 2340
tctattttgt cttgaaatgg cacatcactg ggctaaccta ataatgtact ccgtagcatc 2400
ttaagtgtgt aactgaagta agatataact gtagttttga cggtgcactt cagcacttgg 2460
ctgcagtaca caccggtaat tctatgtgcc agcaagcacg ttacaacgtt cggttggata 2520
gaaactagta gtacacgaca tgacatgcct agatgctact tctacatttt ggtcggtcga 2580
tctgacgtac gtacctacta ggagtactac tcagctcaat taacatgatg ttcatacgat 2640
ggcgccaaaa catcttatac gtacatacgt aggtgtacca tcgtactata cactagacgg 2700
taggtgcaac tcctgctaca tacatgtggc agtcacatct tgccgtcttg tctgacgccg 2760
cgacgtgtat atgatcagcc aacaagtcga cgacgggggc ggccttctcg ccgagcgccg 2820
gtggcttcag ctgccttcag cagcagcagc agcagcatga gatcgacaac ggcggccagt 2880
cgagcaacgc cacgccgact aacctgtccc tggcggcgcc accgccaccg ccgccgcctc 2940
aagacgacgc cggcttcggc gccggccttg acaccatgct gctgattcag cagcaagggc 3000
ccgatgaaca ggaggaggag gaggagcagc atttcatgat gacctctctc gtgcagtcgc 3060
atcgccagca gcagcagcac ggcgacagcg gcaacatctt atcgtgctcg acgacgtcgc 3120
cgtcggatca gcgtcgccat cagaacgacg gcgacagctg ctgcaacggc aacagcatgc 3180
agcatttctt tgaggtggag ttcatgtagc acgatgcatg catgcactgc atgaatgaac 3240
ctggcctagc ctagcctaga ggtggtttga ctgcattatc gtgaggtgtg tgtgcatgag 3300
gtgaaagaga aaacaagctg gttgcatgca ctaggctgtg catggagtat agctactagg 3360
gtacgtaggc gtgcaggctt tttctttcca gatttaattt cctgaccttt tctgcatact 3420
gtgtttttac ttgtactttt ctcagtgtta ccctcttaca cgg 3463
<210> 2
<211> 3463
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 2
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatccgcccc agatttgggc tagatataag agatgtcggt cagcctgggg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc catcattgcc tctctccttc 360
tccggcctcc acgacctttt tagcctgaag gcacgcacaa ccgttcctgc tgcacagaca 420
catactccgg cggtccggcc tctttctttc ttacccatca gtcaggccgc gcgtcaccac 480
ctttctcatc cgcgctcgga atagccggcc atgatgagca gcaggctaag cggcggcacg 540
atggcaccgg ttagcgacat ggccgacttc ggttgcgctc ccatgcagtc ctaccccaac 600
tttgagccgg ccggcatggt catgcccggg gaccggcagc cgcccttcca gcaccaccac 660
ctctacgaca gcctcgactt caacgccgct gcattctcgt tccaagaccc ggtcgcgctc 720
ttctccagcg gctcggcgtt cagtaaccag ctccagcagc cgttcctcca gacgcaggtc 780
accacgccga cgatggcgtc gtcgtcgctg ctgcaggcgc cgatgatgac ccttccgggt 840
atgctgacgt cttcctcggc gtcgccggtg gacgcatgca ccttcggtgg cggcggcagt 900
gctgggttcc tgaagcagga ggagggcggc cccttctcag acgtcggcgg tggggggagg 960
atcgggctga acctcggccg caggacatac ttttccccgg cggacgtgct ggccgtggac 1020
cgcctgctga tgcgctcccg cttcggcgga gccggcgcaa tgggcatgct ggggctgggg 1080
ctcggcgccg ccgcccacca ccaccagacc ccgcggtgcc aggctgaggg ctgcaaggcc 1140
gacctctccg ccgccaagca ctaccaccgc cgccacaagg tctgcgagta ccacgccaag 1200
gccaccacag tcgccgcctc tggcaagcag cagcgcttct gccaacaatg cagccggtat 1260
gtacgtttat cctctgccat gggtggatcg ttgatgcatg cgaagctttg ctagcagacg 1320
aaaaaatcag cagtagaaag gctgcgagct ttcgatctag tggcattgac gctagcagct 1380
atatgcactg atcatatcat tcatgtcaac atgtcatata tcagaaatgg cgcacatcag 1440
atcagatctt gttcctctga gcgtatcggc atctgcatcc ctttgtccaa tgtactacta 1500
gcacgcccat catggcattg cagtggagtt cgatcagata cctaaaccta gtctgtgtag 1560
atacatcata catggcgtgt attacttaca acggtagaca tgtatatacc caagatccgg 1620
ttgtgtgaat ataactggtt cttgtttggg tacgtttgtt gggctaggtt tcatgtgctc 1680
gctgagtttg acgaggccaa gaggagctgc cggaagcggc tcacggagca caaccggcgc 1740
cgccgaaagc ctgccggcgc gcagggcaag gattcgccgc cgccttccaa gaaggcagac 1800
gctagcatca ccagctcata caccggcgat cacaagagta agaccactcc tctgatacat 1860
gtatagactt aaacactgga tatatgctta ttattggctt ttttaaagct tgatcacttt 1920
aagaagttgt gttttgattt tcggccgcaa aatgtggatt tcggccgaat ttcggtcatc 1980
tcggcttgag gcgaaaagta tatgtaggcc gaaattactc aaatttggta aattttggtc 2040
aaatttaagt caaattgcag tcaaaatttg atcaaatttc agccgaaaat tttgaaaatg 2100
gccgaaattc ggccatctcg gcctagggcg aaaaaaagct caaaccgaaa atcaaaacac 2160
agccagtaag ctagctagct acctagaaac tcctgaaact ttgttagagc ttagtgggta 2220
atttgaacta aaagcatgac acttcttttg caacaaggga acatttcaat aacgtatacc 2280
aagtaaagaa cacaacaaaa acagtttgcc taattcttaa ttgtccgaaa atttagtaaa 2340
tctattttgt cttgaaatgg cacatcactg ggctaaccta ataatgtact ccgtagcatc 2400
ttaagtgtgt aactgaagta agatataact gtagttttga cggtgcactt cagcacttgg 2460
ctgcagtaca caccggtaat tctatgtgcc agcaagcacg ttacaacgtt cggttggata 2520
gaaactagta gtacacgaca tgacatgcct agatgctact tctacatttt ggtcggtcga 2580
tctgacgtac gtacctacta ggagtactac tcagctcaat taacatgatg ttcatacgat 2640
ggcgccaaaa catcttatac gtacatacgt aggtgtacca tcgtactata cactagacgg 2700
taggtgcaac tcctgctaca tacatgtggc agtcacatct tgccgtcttg tctgacgccg 2760
cgacgtgtat atgatcagcc aacaagtcga cgacgggggc ggccttctcg ccgagcgccg 2820
gtggcttcag ctgccttcag cagcagcagc agcagcatga gatcgacaac ggcggccagt 2880
cgagcaacgc cacgccgact aacctgtccc tggcggcgcc accgccaccg ccgccgcctc 2940
aagacgacgc cggcttcggc gccggccttg acaccatgct gctgattcag cagcaagggc 3000
ccgatgaaca ggaggaggag gaggagcagc atttcatgat gacctctctc gtgcagtcgc 3060
atcgccagca gcagcagcac ggcgacagcg gcaacatctt atcgtgctcg acgacgtcgc 3120
cgtcggatca gcgtcgccat cagaacgacg gcgacagctg ctgcaacggc aacagcatgc 3180
agcatttctt tgaggtggag ttcatgtagc acgatgcatg catgcactgc atgaatgaac 3240
ctggcctagc ctagcctaga ggtggtttga ctgcattatc gtgaggtgtg tgtgcatgag 3300
gtgaaagaga aaacaagctg gttgcatgca ctaggctgtg catggagtat agctactagg 3360
gtacgtaggc gtgcaggctt tttctttcca gatttaattt cctgaccttt tctgcatact 3420
gtgtttttac ttgtactttt ctcagtgtta ccctcttaca cgg 3463
<210> 3
<211> 3463
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 3
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatccgcccc agatttgggc tagatataag agatgtcggt cagcctggcg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc catcattgcc tctctccttc 360
tccggcctcc acgacctttt tagcctgaag gcacgcacaa ccgttcctgc tgcacagaca 420
catactccgg cggtccggcc tctttctttc ttacccatca gtcaggccgc gcgtcaccac 480
ctttctcatc cgcgctcgga atagccggcc atgatgagca gcaggctaag cggcggcacg 540
atggcaccgg ttagcgacat ggccgacttc ggttgcgctc ccatgcagtc ctaccccaac 600
tttgagccgg ccggcatggt catgcccggg gaccggcagc cgcccttcca gcaccaccac 660
ctctacgaca gcctcgactt caacgccgct gcattctcgt tccaagaccc ggtcgcgctc 720
ttctccagcg gctcggcgtt cagtaaccag ctccagcagc cgttcctcca gacgcaggtc 780
accacgccga cgatggcgtc gtcgtcgctg ctgcaggcgc cgatgatgac ccttccgggt 840
atgctgacgt cttcctcggc gtcgccggtg gacgcatgca ccttcggtgg cggcggcagt 900
gctgggttcc tgaagcagga ggagggcggc cccttctcag acgtcggcgg tggggggagg 960
atcgggctga acctcggccg caggacatac ttttccccgg cggacgtgct ggccgtggac 1020
cgcctgctga tgcgctcccg cttcggcgga gccggcgcaa tgggcatgct ggggctgggg 1080
ctcggcgccg ccgcccacca ccaccagacc ccgcggtgcc aggctgaggg ctgcaaggcc 1140
gacctctccg ccgccaagca ctaccaccgc cgccacaagg tctgcgagta ccacgccaag 1200
gccaccacag tcgccgcctc tggcaagcag cagcgcttct gccaacaatg cagccggtat 1260
gtacgtttat cctctgccat gggtggatcg ttgatgcatg cgaagctttg ctagcagacg 1320
aaaaaatcag cagtagaaag gctgcgagct ttcgatctag tggcattgac gctagcagct 1380
atatgcactg atcatatcat tcatgtcaac atgtcatata tcagaaatgg cgcacatcag 1440
atcagatctt gttcctctga gcgtatcggc atctgcatcc ctttgtccaa tgtactacta 1500
gcacgcccat catggcattg cagtggagtt cgatcagata cctaaaccta gtctgtgtag 1560
atacatcata catggcgtgt attacttaca acggtagaca tgtatatacc caagatccgg 1620
ttgtgtgaat ataactggtt cttgtttggg tacgtttgtt gggctaggtt tcatgtgctc 1680
gctgagtttg acgaggccaa gaggagctgc cggaagcggc tcacggagca caaccggcgc 1740
cgccgaaagc ctgccggcgc gcagggcaag gattcgccgc cgccttccaa gaaggcagac 1800
gctagcatca ccagctcata caccggcgat cacaagagta agaccactcc tctgatacat 1860
gtatagactt aaacactgga tatatgctta ttattggctt ttttaaagct tgatcacttt 1920
aagaagttgt gttttgattt tcggccgcaa aatgtggatt tcggccgaat ttcggtcatc 1980
tcggcttgag gcgaaaagta tatgtaggcc gaaattactc aaatttggta aattttggtc 2040
aaatttaagt caaattgcag tcaaaatttg atcaaatttc agccgaaaat tttgaaaatg 2100
gccgaaattc ggccatctcg gcctagggcg aaaaaaagct caaaccgaaa atcaaaacac 2160
agccagtaag ctagctagct acctagaaac tcctgaaact ttgttagagc ttagtgggta 2220
atttgaacta aaagcatgac acttcttttg caacaaggga acatttcaat aacgtatacc 2280
aagtaaagaa cacaacaaaa acagtttgcc taattcttaa ttgtccgaaa atttagtaaa 2340
tctattttgt cttgaaatgg cacatcactg ggctaaccta ataatgtact ccgtagcatc 2400
ttaagtgtgt aactgaagta agatataact gtagttttga cggtgcactt cagcacttgg 2460
ctgcagtaca caccggtaat tctatgtgcc agcaagcacg ttacaacgtt cggttggata 2520
gaaactagta gtacacgaca tgacatgcct agatgctact tctacatttt ggtcggtcga 2580
tctgacgtac gtacctacta ggagtactac tcagctcaat taacatgatg ttcatacgat 2640
ggcgccaaaa catcttatac gtacatacgt aggtgtacca tcgtactata cactagacgg 2700
taggtgcaac tcctgctaca tacatgtggc agtcacatct tgccgtcttg tctgacgccg 2760
cgacgtgtat atgatcagcc aacaagtcga cgacgggggc ggccttctcg ccgagcgccg 2820
gtggcttcag ctgccttcag cagcagcagc agcagcatga gatcgacaac ggcggccagt 2880
cgagcaacgc cacgccgact aacctgtccc tggcggcgcc accgccaccg ccgccgcctc 2940
aagacgacgc cggcttcggc gccggccttg acaccatgct gctgattcag cagcaagggc 3000
ccgatgaaca ggaggaggag gaggagcagc atttcatgat gacctctctc gtgcagtcgc 3060
atcgccagca gcagcagcac ggcgacagcg gcaacatctt atcgtgctcg acgacgtcgc 3120
cgtcggatca gcgtcgccat cagaacgacg gcgacagctg ctgcaacggc aacagcatgc 3180
agcatttctt tgaggtggag ttcatgtagc acgatgcatg catgcactgc atgaatgaac 3240
ctggcctagc ctagcctaga ggtggtttga ctgcattatc gtgaggtgtg tgtgcatgag 3300
gtgaaagaga aaacaagctg gttgcatgca ctaggctgtg catggagtat agctactagg 3360
gtacgtaggc gtgcaggctt tttctttcca gatttaattt cctgaccttt tctgcatact 3420
gtgtttttac ttgtactttt ctcagtgtta ccctcttaca cgg 3463
<210> 4
<211> 20
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 4
ggatagtgca gtgaagcggt 20
<210> 5
<211> 24
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 5
tggcatctct tgggctctt 19
<210> 6
<211> 342
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 6
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatctgcccc agatttgggc tagatataag agatgtcggc cagcctggcg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc ca 342
<210> 7
<211> 342
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 7
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatccgcccc agatttgggc tagatataag agatgtcggt cagcctgggg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc ca 342
<210> 8
<211> 342
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 8
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatccgcccc agatttgggc tagatataag agatgtcggt cagcctggcg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc ca 342
<210> 9
<211> 3463
<212> DNA
<213>Artificial sequence
<220>
<221>
<222> (125)…(125) (160)…(160) (169)…(169)
<223>N is that c or t r are c or g
<400> 9
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatcngcccc agatttgggc tagatataag agatgtcggn cagcctggrg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc catcattgcc tctctccttc 360
tccggcctcc acgacctttt tagcctgaag gcacgcacaa ccgttcctgc tgcacagaca 420
catactccgg cggtccggcc tctttctttc ttacccatca gtcaggccgc gcgtcaccac 480
ctttctcatc cgcgctcgga atagccggcc atgatgagca gcaggctaag cggcggcacg 540
atggcaccgg ttagcgacat ggccgacttc ggttgcgctc ccatgcagtc ctaccccaac 600
tttgagccgg ccggcatggt catgcccggg gaccggcagc cgcccttcca gcaccaccac 660
ctctacgaca gcctcgactt caacgccgct gcattctcgt tccaagaccc ggtcgcgctc 720
ttctccagcg gctcggcgtt cagtaaccag ctccagcagc cgttcctcca gacgcaggtc 780
accacgccga cgatggcgtc gtcgtcgctg ctgcaggcgc cgatgatgac ccttccgggt 840
atgctgacgt cttcctcggc gtcgccggtg gacgcatgca ccttcggtgg cggcggcagt 900
gctgggttcc tgaagcagga ggagggcggc cccttctcag acgtcggcgg tggggggagg 960
atcgggctga acctcggccg caggacatac ttttccccgg cggacgtgct ggccgtggac 1020
cgcctgctga tgcgctcccg cttcggcgga gccggcgcaa tgggcatgct ggggctgggg 1080
ctcggcgccg ccgcccacca ccaccagacc ccgcggtgcc aggctgaggg ctgcaaggcc 1140
gacctctccg ccgccaagca ctaccaccgc cgccacaagg tctgcgagta ccacgccaag 1200
gccaccacag tcgccgcctc tggcaagcag cagcgcttct gccaacaatg cagccggtat 1260
gtacgtttat cctctgccat gggtggatcg ttgatgcatg cgaagctttg ctagcagacg 1320
aaaaaatcag cagtagaaag gctgcgagct ttcgatctag tggcattgac gctagcagct 1380
atatgcactg atcatatcat tcatgtcaac atgtcatata tcagaaatgg cgcacatcag 1440
atcagatctt gttcctctga gcgtatcggc atctgcatcc ctttgtccaa tgtactacta 1500
gcacgcccat catggcattg cagtggagtt cgatcagata cctaaaccta gtctgtgtag 1560
atacatcata catggcgtgt attacttaca acggtagaca tgtatatacc caagatccgg 1620
ttgtgtgaat ataactggtt cttgtttggg tacgtttgtt gggctaggtt tcatgtgctc 1680
gctgagtttg acgaggccaa gaggagctgc cggaagcggc tcacggagca caaccggcgc 1740
cgccgaaagc ctgccggcgc gcagggcaag gattcgccgc cgccttccaa gaaggcagac 1800
gctagcatca ccagctcata caccggcgat cacaagagta agaccactcc tctgatacat 1860
gtatagactt aaacactgga tatatgctta ttattggctt ttttaaagct tgatcacttt 1920
aagaagttgt gttttgattt tcggccgcaa aatgtggatt tcggccgaat ttcggtcatc 1980
tcggcttgag gcgaaaagta tatgtaggcc gaaattactc aaatttggta aattttggtc 2040
aaatttaagt caaattgcag tcaaaatttg atcaaatttc agccgaaaat tttgaaaatg 2100
gccgaaattc ggccatctcg gcctagggcg aaaaaaagct caaaccgaaa atcaaaacac 2160
agccagtaag ctagctagct acctagaaac tcctgaaact ttgttagagc ttagtgggta 2220
atttgaacta aaagcatgac acttcttttg caacaaggga acatttcaat aacgtatacc 2280
aagtaaagaa cacaacaaaa acagtttgcc taattcttaa ttgtccgaaa atttagtaaa 2340
tctattttgt cttgaaatgg cacatcactg ggctaaccta ataatgtact ccgtagcatc 2400
ttaagtgtgt aactgaagta agatataact gtagttttga cggtgcactt cagcacttgg 2460
ctgcagtaca caccggtaat tctatgtgcc agcaagcacg ttacaacgtt cggttggata 2520
gaaactagta gtacacgaca tgacatgcct agatgctact tctacatttt ggtcggtcga 2580
tctgacgtac gtacctacta ggagtactac tcagctcaat taacatgatg ttcatacgat 2640
ggcgccaaaa catcttatac gtacatacgt aggtgtacca tcgtactata cactagacgg 2700
taggtgcaac tcctgctaca tacatgtggc agtcacatct tgccgtcttg tctgacgccg 2760
cgacgtgtat atgatcagcc aacaagtcga cgacgggggc ggccttctcg ccgagcgccg 2820
gtggcttcag ctgccttcag cagcagcagc agcagcatga gatcgacaac ggcggccagt 2880
cgagcaacgc cacgccgact aacctgtccc tggcggcgcc accgccaccg ccgccgcctc 2940
aagacgacgc cggcttcggc gccggccttg acaccatgct gctgattcag cagcaagggc 3000
ccgatgaaca ggaggaggag gaggagcagc atttcatgat gacctctctc gtgcagtcgc 3060
atcgccagca gcagcagcac ggcgacagcg gcaacatctt atcgtgctcg acgacgtcgc 3120
cgtcggatca gcgtcgccat cagaacgacg gcgacagctg ctgcaacggc aacagcatgc 3180
agcatttctt tgaggtggag ttcatgtagc acgatgcatg catgcactgc atgaatgaac 3240
ctggcctagc ctagcctaga ggtggtttga ctgcattatc gtgaggtgtg tgtgcatgag 3300
gtgaaagaga aaacaagctg gttgcatgca ctaggctgtg catggagtat agctactagg 3360
gtacgtaggc gtgcaggctt tttctttcca gatttaattt cctgaccttt tctgcatact 3420
gtgtttttac ttgtactttt ctcagtgtta ccctcttaca cgg 3463
<210> 10
<211> 342
<212> DNA
<213>Artificial sequence
<220>
<221>
<222> (125)…(125) (160)…(160) (169)…(169)
<223>N is that c or t r are c or g
<400> 10
ggatagtgca gtgaagcggt tagggtttgg tccgacgaat ggatgaaaag gaatagacgt 60
ggggtcgggt gggctagcgt ggaccgggtc cgacgtggcg gacacgcccg agcgtcccct 120
tatcngcccc agatttgggc tagatataag agatgtcggn cagcctggrg tttgagatcc 180
gtttaagacg ttcgtgtggg ttgaattttt gtgaccaggt cgttcgtccg cacatttaga 240
acgggttcga ggcgtcccag cgtggatgct ggagttgaga tttaggtggg gtcgtttgcg 300
cagataaagg acgggagaag gccaagagcc caagagatgc ca 342
Claims (10)
1. a kind of method of screening or assisting sifting difference plant height wheat, comprises the following steps：Detect that wheat to be measured is based on
The genotype of TaSPL21-6A genes is genotype HapI, genotype HapII or genotype HapIII, and genotype HapI's is small
The plant height of wheat>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII；
The wheat of the genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, based on T160C SNP
The genotype selected is that CC is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；
The wheat of the genotype HapII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP
The genotype in site is that TT is homozygous and genotype based on C169G SNP sites is GG homozygous wheat；
The wheat of the genotype HapIII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP
The genotype in site is that TT is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；
" the T125C SNP sites " the 125th nucleotides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles；
" the T160C SNP sites " the 160th nucleotides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles；It is described
" C169G SNP sites " the 169th nucleotides from 5 ' ends for the sequence 9 in sequence table in Wheat volatiles.
2. a kind of method of screening or assisting sifting difference plant height wheat, comprises the following steps：Detect that wheat to be measured is based on
The genotype of TaSPL21-6A genes is genotype HapI, genotype HapII or genotype HapIII, and genotype HapI's is small
The plant height of wheat>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII；
The wheat of the genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, based on T160C SNP
The genotype selected is that CC is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；
The wheat of the genotype HapII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP
The genotype in site is that TT is homozygous and genotype based on C169G SNP sites is GG homozygous wheat；
The wheat of the genotype HapIII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP
The genotype in site is that TT is homozygous and genotype based on C169G SNP sites is CC homozygous wheat；
" the T125C SNP sites " the 125th nucleotides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles；
" the T160C SNP sites " the 160th nucleotides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles；It is described
" C169G SNP sites " the 169th nucleotides from 5 ' ends for the sequence 10 in sequence table in Wheat volatiles.
3. a kind of method of screening or assisting sifting difference plant height wheat, in turn includes the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded, and is obtained by the primer pair constituted using primers F and primer R
To pcr amplification product；
(2) pcr amplification product is carried out into digestion with restriction enzyme BglI, obtains digestion products A；The PCR is expanded
Volume increase thing carries out digestion with restriction enzyme BseDI or SecI, obtains digestion products B；
(3) judged as follows：
If the digestion products A is for two DNA fragmentations and the digestion products B is a DNA fragmentation, wheat to be measured is based on
The genotype of TaSPL21-6A genes is genotype HapI；
If the digestion products A is for a DNA fragmentation and the digestion products B is two DNA fragmentations, wheat to be measured is based on
The genotype of TaSPL21-6A genes is genotype HapII；
If the digestion products A is for a DNA fragmentation and the digestion products B is a DNA fragmentation, wheat to be measured is based on
The genotype of TaSPL21-6A genes is genotype HapIII；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of energy；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of energy.
4. a kind of method of screening or assisting sifting difference plant height wheat, in turn includes the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded, and is obtained by the primer pair constituted using primers F and primer R
To pcr amplification product；
(2) pcr amplification product is carried out into digestion with restriction enzyme BglI, obtains digestion products A；The PCR is expanded
Volume increase thing carries out digestion with restriction enzyme BseDI or SecI, obtains digestion products B；
(3) judged as follows：
If had during there is the DNA fragmentation of 176bp and the DNA fragmentation and the digestion products B of 166bp in digestion products A
The DNA fragmentation of 342bp, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapI；
If there is the digestion products A DNA fragmentation and digestion products B of 342bp to have the DNA fragmentation and 177bp of 165bp
DNA fragmentation, then the genotype that wheat to be measured is based on TaSPL21-6A genes is genotype HapII；
If there is the digestion products A DNA fragmentation and digestion products B of 342bp to have the DNA fragmentation of 342bp, to be measured small
McGee is genotype HapIII in the genotype of TaSPL21-6A genes；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of energy；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of energy.
5. a kind of method of screening or assisting sifting difference plant height wheat, in turn includes the following steps：
(1) genomic DNA with wheat to be measured as template, entered performing PCR and expanded, and is obtained by the primer pair constituted using primers F and primer R
To pcr amplification product；
(2) pcr amplification product is sequenced；
(3) judged as follows：
If the nucleotide sequence of the pcr amplification product is as shown in the sequence 1 the 1st to 342 from 5' ends in sequence table,
The genotype that then wheat to be measured is based on TaSPL21-6A genes is genotype HapI；
If the nucleotide sequence of the pcr amplification product is as shown in the sequence 2 the 1st to 342 from 5' ends in sequence table,
The genotype that then wheat to be measured is based on TaSPL21-6A genes is genotype HapII；
If the nucleotide sequence of the pcr amplification product is as shown in the sequence 3 the 1st to 342 from 5' ends in sequence table,
The genotype that then wheat to be measured is based on TaSPL21-6A genes is genotype HapIII；
The plant height of the wheat of genotype HapI>The plant height of the wheat of genotype HapIII>The plant height of the wheat of genotype HapII；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of energy；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of energy.
6. a kind of kit identified or aid in identification Plant Height in Wheat, including detection wheat to be measured is based on TaSPL21-6A genes
Genotype is the material of genotype HapI, genotype HapII or genotype HapIII；
The genotype HapI is the genotype based on T125C SNP sites for TT is homozygous, base based on T160C SNP sites
Because type is that CC is homozygous and genotype based on C169G SNP sites is CC homozygous TaSPL21-6A genes；
The genotype HapII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP sites
Genotype is that TT is homozygous and genotype based on C169G SNP sites is GG homozygous TaSPL21-6A genes；
The genotype HapIII is the genotype based on T125C SNP sites for CC is homozygous, based on T160C SNP sites
Genotype is that TT is homozygous and genotype based on C169G SNP sites is CC homozygous TaSPL21-6A genes；
" the T125C SNP sites " is that the sequence 9 in sequence table in Wheat volatiles or the sequence 10 in sequence table are last from 5 '
The 125th nucleotides is held；" the T160C SNP sites " is in the sequence 9 in sequence table in Wheat volatiles or sequence table
Sequence 10 from 5 ' ends the 160th nucleotides；" the C169G SNP sites " is the sequence in sequence table in Wheat volatiles
Sequence 10 in row 9 or sequence table the 169th nucleotides from 5 ' ends.
7. kit as claimed in claim 6, it is characterised in that：It is described that " detection wheat to be measured is based on TaSPL21-6A genes
Genotype be the material of genotype HapI, genotype HapII or genotype HapIII " be drawing that primers F and primer R are constituted
Thing pair；
The primers F is following a1) or a2)：
A1) the single strand dna shown in the sequence 4 of sequence table；
A2 sequence 4) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 4
The DNA molecular of energy；
The primer R is following a3) or a4)：
A3) the single strand dna shown in the sequence 5 of sequence table；
A4 sequence 5) had into identical work(by the substitution of one or several nucleotides and/or missing and/or addition and with sequence 5
The DNA molecular of energy.
8. molecular labeling first, as shown in the sequence 10 of sequence table.
9. molecular labeling second, as shown in the sequence 9 of sequence table.
10. (z1) or (z2) or (z3) or (z4) or (z5)：
(z1) kit described in claim 6 or 7 or, molecular labeling first described in claim 8 or, described in claim 8 point
Application of the son mark second in screening or assisting sifting difference plant height wheat；
(z2) kit described in claim 6 or 7 or, molecular labeling first described in claim 8 or, described in claim 8 point
Application of the son mark second in the product for preparing screening or assisting sifting difference plant height wheat；
(z3) kit described in claim 6 or 7 or, molecular labeling first described in claim 8 or, described in claim 8 point
Application of the son mark second in the genotype of identification wheat TaSPL21-6A genes；
(z4) kit described in claim 6 or 7 or, molecular labeling first described in claim 8 or, described in claim 8 point
Application of the son mark second in the product of genotype of identification wheat TaSPL21-6A genes is prepared；
(z5) kit described in claim 6 or 7 or, molecular labeling first described in claim 8 or, described in claim 8 point
Application of the son mark second in wheat breeding.
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| CN109439788A (en) * | 2018-11-20 | 2019-03-08 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | KASP molecular labeling and its application with Plant Height in Wheat major gene loci close linkage |
| CN115992290A (en) * | 2022-12-15 | 2023-04-21 | 河北师范大学 | Wheat plant height related SNP locus and application thereof in auxiliary screening of wheat with different plant heights |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858445A (en) * | 2017-11-20 | 2018-03-30 | 东北农业大学 | A kind of method and its dedicated kit for screening the soybean varieties with different growth stage and plant height |
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| CN108546776A (en) * | 2018-07-16 | 2018-09-18 | 四川农业大学 | The InDel labels and identification method of a kind of Triticum polonicum L of short stem and application |
| CN109439788A (en) * | 2018-11-20 | 2019-03-08 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | KASP molecular labeling and its application with Plant Height in Wheat major gene loci close linkage |
| CN109439788B (en) * | 2018-11-20 | 2022-05-13 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | KASP molecular marker closely linked with major gene locus of wheat plant height and application thereof |
| CN115992290A (en) * | 2022-12-15 | 2023-04-21 | 河北师范大学 | Wheat plant height related SNP locus and application thereof in auxiliary screening of wheat with different plant heights |
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