CN106754299A - Microbial inoculant pin - Google Patents
Microbial inoculant pin Download PDFInfo
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- CN106754299A CN106754299A CN201611231529.XA CN201611231529A CN106754299A CN 106754299 A CN106754299 A CN 106754299A CN 201611231529 A CN201611231529 A CN 201611231529A CN 106754299 A CN106754299 A CN 106754299A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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Abstract
The present invention provides microbial inoculant pin, including main needle tubing, be connected on main needle tubing syringe needle, slidably plug is promoted mainly in the main needle tubing, also including pushing pin and being inoculated with pair pin.Push pin slidably to be located at and promote mainly in Sai Nei and syringe needle；On main needle tubing, the secondary pin of inoculation includes the secondary needle tubing of inoculation connected with main needle tubing and the slidably secondary plunger in the secondary needle tubing of inoculation to the secondary pin of at least one inoculation.Microbial inoculant pin of the invention is the form of trocar in main needle tubing, the secondary pin of inoculation is added on main needle tubing, by microorganism storage in secondary needle tubing, on the one hand it is easy to add microorganism in secondary needle tubing, on the other hand a certain amount of microorganism can be stored in the secondary pin of inoculation, can be repeatedly inoculated with, without repeatedly, it is troublesome dress take microorganism, be it is a kind of can vaccinization, microorganism dress take convenient, time saving and energy saving, inoculation efficiency microbial inoculant pin high.
Description
Technical field
The present invention relates to syringe art, in particular to a kind of microbial inoculant pin.
Background technology
In medicine, the research and development and screening and assessment of antineoplastic, qualified animal tumor transplanting is set up
Model is of crucial importance for further studying, and the foundation of animal tumor transplantation model has various methods, wherein fritter inocalation method because
Tumour survival rate is high after its inoculation and tumour growth volume can be made bigger, using relatively broad.
Fritter inocalation method is to take out tumor tissues in vivo from the animal (mostly mouse) of conservation, by tumor tissues clipping into
Fritter (about 5 × 5 × 5mm), is put into inoculation position (being often animal oxter), and the method that tumor tissues are put into animal inoculation pvaccination position has
Two kinds, one kind is that an osculum is cut off at receptor armpit, and it is subendothelial to grip tumor tissues fritter feeding armpit with tweezers.
Another method is to be filled in trochar tumor tissues fritter with trochar, trochar is penetrated from animal flank portion, edge
It is subcutaneous to walk to oxter, then tumor tissues fritter is released into inoculation from trochar.
Being inoculated with receptor by traditional tumor tissue's inoculation method has place not fully up to expectations, is pressed from both sides with tweezers
Enter tumor tissues fritter, it is larger that one side animal armpit incision needs are opened, and the use of tweezers can make animal armpit incision more
Expand, tumor tissue easily spills, be difficult growth, it is slow-growing after tumor inoculation.When using trochar, trochar is without standard measure
Tissue volume, tumor tissue's loading trochar is more troublesome and time-consuming, and the tumor tissues for loading every time can only give one
Animal, it is impossible to vaccinization, therefore in inoculation in the presence of inconsistent scarce of time-consuming, laborious, every animal tumor tissue inoculum concentration
Point.
The content of the invention
In view of this, the invention aims to overcome deficiency of the prior art, there is provided be that one kind can continuously connect
Plant, microorganism dress takes convenient, time saving and energy saving, inoculation efficiency microbial inoculant pin high.
To solve the above problems, the solution that the present invention is provided is as follows：
Microbial inoculant pin, including main needle tubing, be connected on the main needle tubing syringe needle, slidably be located at the main needle tubing
Interior promotes mainly plug；
Also include pushing pin and being inoculated with secondary pin, described pushing pin slidably is promoted mainly in Sai Nei and the syringe needle located at described；
Secondary pin is inoculated with described at least one on the main needle tubing, the secondary pin of inoculation includes connecting with the main needle tubing
The secondary needle tubing of logical inoculation and the slidably secondary plunger in the secondary needle tubing of the inoculation.
In the exemplary embodiment, length of the length for promoting mainly plug not less than the main needle tubing；
The length pushed pin is more than the length sum for promoting mainly plug and the syringe needle.
In the exemplary embodiment, described pushing pin is provided with limiting section；
The limiting section with it is described promote mainly plug and abut when, it is described push pin from it is described promote mainly plug in the length stretched out more than described in
The length of syringe needle.
In the exemplary embodiment, for hollow tube, the hollow hole is engaged the plug of promoting mainly with described pushing pin；
The limiting section is the projection for being provided close to the end of pushing pin, and is pushed pin described in the raised limitation and is promoted mainly to described
Slided in plug.
In the exemplary embodiment, each secondary needle tubing is marked with volume markings.
In the exemplary embodiment, each secondary needle tubing is detachable with the main needle tubing.
In the exemplary embodiment, the secondary needle tubing is connected through a screw thread with the main needle tubing.
In the exemplary embodiment, the secondary needle tubing is connected with the main needle tubing by clamping.
In the exemplary embodiment, the endoporus of the syringe needle is engaged with described pushing pin.
In the exemplary embodiment, the syringe needle is detachable with the main needle tubing.
Microbial inoculant pin of the invention is the form of trocar in main needle tubing, and the secondary pin of inoculation is added on main needle tubing, will be micro-
Biometric storage is easy in secondary needle tubing, on the one hand to microorganism is added in secondary needle tubing, on the other hand can be stored in the secondary pin of inoculation
A certain amount of microorganism, can be repeatedly inoculated with, without repeatedly, it is troublesome dress take microorganism, be that one kind can continuously connect
Plant, microorganism dress takes convenient, time saving and energy saving, inoculation efficiency microbial inoculant pin high.
Volume markings are labeled with the secondary needle tubing of the secondary pin of inoculation, can make to be injected by the secondary pin of inoculation by the structure of trocar
Microorganism all released from syringe needle, thus the microbial biomass injected in the secondary pin of inoculation is inoculum concentration, is that one kind can be quantified
Injection, the microbial inoculant pin that effect of inoculation is good.
Being detachably connected between the secondary pin of inoculation and main needle tubing so that the dress extract operation of microorganism is easier, dress takes effect
Rate is higher.
For enable the above objects, features and advantages of the present invention become apparent from understandable, preferred embodiment cited below particularly, and match somebody with somebody
Appended accompanying drawing is closed, is described below in detail.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, below will be attached to what is used needed for embodiment
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, thus be not construed as it is right
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 shows that the decomposition texture of the microbial inoculant pin that embodiment of the present invention first embodiment is provided is illustrated
Figure；
Fig. 2 shows that the overall structure of the microbial inoculant pin that embodiment of the present invention first embodiment is provided is illustrated
Figure；
Fig. 3 shows that the overall structure of the microbial inoculant pin that embodiment of the present invention second embodiment is provided is illustrated
Figure；
Fig. 4 shows that the overall structure of the microbial inoculant pin that the implementation method of the embodiment of the present invention the 3rd is provided is illustrated
Figure.
Main element symbol description：
1- microbial inoculant pins；The main needle tubings of 11-；111- interfaces；12- syringe needles；13- promotes mainly plug；14- pushes pin；141- is spacing
Portion；The secondary pin of 15- inoculations；151- pair needle tubings；152- pair plungers.
Specific embodiment
For the ease of understanding the present invention, microbial inoculant pin is described more fully below with reference to relevant drawings.
The preferred embodiment of microbial inoculant pin is given in accompanying drawing.But, microbial inoculant pin can be by many different forms
To realize, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is made to microbial inoculant
The disclosure of pin is more thorough comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or may be simultaneously present centering elements.Conversely, when element be referred to as " directly existing " another element " on " when,
In the absence of intermediary element.Term as used herein " vertical ", " level ", "left", "right" and similar statement are
For illustrative purposes.
Unless otherwise defined, all of technologies and scientific terms used here by the article with belong to technical field of the invention
The implication that technical staff is generally understood that is identical.The term used in the description in microbial inoculant pin is intended merely to herein
The purpose of specific embodiment is described, it is not intended that the limitation present invention.Term as used herein " and/or " include one or many
The arbitrary and all of combination of individual related Listed Items.
Below in conjunction with the accompanying drawings, specific embodiment of the invention is elaborated.
Embodiment 1
Also referring to Fig. 1 and Fig. 2, microbial inoculant pin 1 is a kind of syringe, is mainly used in vaccine inoculation to prevent disease
Disease or inoculated and cultured tissue are treating disease.This microbial inoculant pin 1 is particularly suited for the inoculation of low dose.The present embodiment it is micro-
Bacterization pin 1 is as a example by mouse inoculation tumor tissues.
Microbial inoculant pin 1 include main needle tubing 11, syringe needle 12, promote mainly plug 13, push pin 14 and the secondary pin 15 of inoculation.Wherein, it is main
The end of needle tubing 11 is connected with syringe needle 12.The inside of main needle tubing 11 is provided with promotes mainly plug 13, promotes mainly plug 13 sliding inside main needle tubing 11
It is dynamic.Promote mainly to be provided with inside plug 13 and push pin 14, push pin and 14 promoting mainly 13 internal slides of plug.The secondary pin 15 of inoculation is connected to main needle tubing 11
On, inoculation pair pin 15 includes the secondary pipe of pin 15 of inoculation and secondary plunger 152, and secondary plunger 152 is managed interior and is being inoculated with located at the secondary pin 15 of inoculation
Secondary pin 15 manages interior slip.The secondary pipe of pin 15 of inoculation is connected with main needle tubing 11.
Main needle tubing 11 is the main body of microbial inoculant pin 1, and all parts are with main needle tubing 11 for main carrier.By master
Needle tubing 11, promote mainly plug 13 and push pin and 14 constitute trocar, promote mainly during plug 13 shifts the injected material in main needle tubing 11 onto syringe needle 12, will
Syringe needle 12 is penetrated from the oxter of inoculation position, i.e. mouse, by push pin 14 by syringe needle 12 dose injection thing inject
To the oxter of mouse.It is used to store injected material, i.e. tumor tissues in the secondary needle tubing 151 for adding, by the inoculation pair pin 15 for adding
While low dose of injection is realized, and can realize disposably loading tumor tissues, be injected to multiple mouse, without
Tumor tissues are added repeatedly, it is time saving and energy saving using more facilitating, while so that the degree of purity of tumor tissues is higher, it is to avoid anti-
Pollution during tumor tissues is added again.
The quantity of the secondary pin 15 of inoculation being connected on main needle tubing 11 is at least one.In the present embodiment, connect on main needle tubing 11
It is connected to two secondary pins 15 of inoculation.The advantage for being connected with least one inoculation pair pin 15 is that the secondary pin 15 of multiple inoculation can be obtained
Bigger tumor tissues inoculation capacity, disposable dress takes can be to more mouse inoculations.The secondary pin of two inoculations of the present embodiment
15, on the one hand meet the continuous capacity requirement to multiple mouse inoculations.On the other hand consider from the layout of reasonably optimizing, not
Under conditions of influence user is to the gripping of main needle tubing 11, two secondary pins 15 of inoculation are used.
Main needle tubing 11 is cylindrical, and its material can select nontoxic plastics, stainless steel or glass etc..The present embodiment
In, main needle tubing 11 is stainless steel metal pipe, and there is stainless steel preferable corrosion resistance to have excellent chemical characteristic, and with excellent
Good mechanical property is extremely durable, main needle tubing 11 sterilized after use can Reusability, reduce cost, at the same reduce it is right
The pollution of environment.The main needle tubing 11 of plastics is disposable needle tubing, is abandoned after use, and disposable main needle tubing 11 is using more
Safety, in the absence of the injected material cross-infection that sterilization cleaning is not caused totally.
The end of main needle tubing 11 is connected with syringe needle 12, and syringe needle 12 is the syringe needle 12 of ordinary syringe, is one hollow
Metal needle, its end is sharp, and for piercing through mouse oxter, on the one hand hollow structure is used to be vented, is on the one hand used to liquid.This
In embodiment, syringe needle 12 is stainless steel syringe needle 12, can Reusability.It is detachable between syringe needle 12 and main needle tubing 11, such as by card
Connect in succession.
Due to syringe needle 12 it is more sharp and it is long-term it is exposed easily cause the damage of needle point outside, influence the puncture of syringe needle 12
Effect, thus syringe needle 12 should be in time changed when syringe needle 12 is damaged, dismountable connected mode so that the replacing of syringe needle 12 is more
It is convenient.Syringe needle 12 and main needle tubing 11 can be reused simultaneously, and cleaning and sterilizing, removable needle 12 are needed after use
With main needle tubing 11 cleaning the moment cleaned respectively so that cleaning more thoroughly comprehensively.
It is the cylindrical, hollow cylinder inside main needle tubing 11 to promote mainly plug 13, promotes mainly the end sleeve rubber sleeve of plug 13.Set
Promoting mainly for rubber sleeve is filled between 13 and the inwall of main needle tubing 11 for interference fit, i.e. rubber sleeve are subject to the inwall of main needle tubing 11
Extruding generates deformation, and elastic-restoring force is generated with the deformation, so as to will promote mainly sealed between plug 13 and main needle tubing 11, leads to
Cross promote mainly plug 13 slip in main needle tubing 11 by main needle tubing 11 tumor tissues extruding and go out.
The length of the length not less than main needle tubing 11 of plug 13 is promoted mainly, that is, promotes mainly the bottom that the rubber on plug 13 is placed on main needle tubing 11
When portion abuts, promote mainly plug 13 and also have part to expose from main needle tubing 11.The advantage of this arrangement is to be longer than the master of main needle tubing 11
Plunger 13 is easy to be pulled out after push-in in main needle tubing 11.
It is hollow to promote mainly be inserted with plug 13 push pin 14, push pin 14 actually solid round rod, its size and syringe needle 12
Matching size is closed.The hollow aperture for promoting mainly plug 13 is engaged with 14 diameter of pushing pin.Push pin 14 promote mainly plug 13 in slide, lead to
Cross and promote mainly during plug 13 pushes syringe needle 12 to, so as to the tumor tissues in syringe needle 12 be extruded.
It should be noted that push pin 14 length compared with promote mainly plug 13 and syringe needle 12 length sum it is long.To ensure to push pin 14
Can be passed in syringe needle 12 or the end face at least with syringe needle 12 is equal, so as to the tumor tissues in syringe needle 12 all be discharged.By
It is less in the inoculum concentration of the tumor tissues to mouse, by the complete emptying discharge of tumor tissues in syringe needle 12 so that microorganism connects
The control for planting the inoculum concentration of pin 1 is more accurate, so as to reach preferable effect of inoculation.
In the present embodiment, 14 end of pushing pin is provided with push handle, and 14 are pushed pin by push handle.Due to pushing pin, 14 diameters are small,
It is relatively thin, thus push-and-pull push pin 14 when be not easy to operation.Pushing pin, 14 end adds push handle structure, increased to push pin and 14 receives
Power area, it is more laborsaving by push handle to 14 forces of pushing pin.
14 limiting section 141 is provided near pushing pin for push handle, limiting section 141 is the projection pushed pin on 14, when limiting section 141
When being connected to the end for promoting mainly plug 13,14 slips in plug 13 is being promoted mainly of pushing pin are limited.Specifically, working as limiting section 141 and promoting mainly
When the end face of plug 13 is abutted, length of 14 length stretched out in plug 13 is promoted mainly of pushing pin more than syringe needle 12.Be ensure that as master with this
When plunger 13 is abutted with main needle tubing 11,14 are pushed pin when shifting limiting section 141 onto and promoting mainly plug 13 and abut, pushing pin 14 can pass completely through pin
First 12, so as to ensure that syringe needle 12 noon tumor tissues are remained, tumor tissues are completely exhausted out.
Specifically, in the present embodiment, the 6cm long of main needle tubing 11, the 5cm long of syringe needle 12, the length for promoting mainly plug 13 is also 6cm, is pushed away
The length of pin 14 is 13cm.Limiting section 141 located at from push pin 14 end 1.6cm away from, it is known that, when limiting section 141 is connected to master
When in plunger 13,14 length stretched out from syringe needle 12 of pushing pin are 13-6-5-1.6=0.4cm, that is, pushing pin 14 can completely by syringe needle
Tumor tissues in 12 are extruded and gone out.
It is appreciated that in other examples, main needle tubing 11, syringe needle 12, promote mainly plug 13 and can also be other sizes,
Different sizes is selected according to different injection demands.
Main needle tubing 11 is provided with to be used to install the secondary pin of inoculation with the secondary quantity identical interface 111 of pin 15 of inoculation, the interface 111
15。
The secondary pin 15 of inoculation is actual to be deposited and to the structure that tumor tissues are injected in main needle tubing 11 for a kind of.Secondary needle tubing 151 is in
Cylinder is filled, and is used to store tumor tissues in secondary needle tubing 151, and its one end is connected with main needle tubing 11, and secondary plunger is inserted with the other end
152.It was between the inwall of the end sleeve rubber sleeve of secondary plunger 152, the secondary plunger 152 of set rubber sleeve and secondary needle tubing 151
Being full of cooperation, i.e. rubber sleeve is subject to the extruding of the inwall of secondary needle tubing 151 to generate deformation, and the adjoint deformation generates elastic recovery
Power, so as to be sealed between secondary plunger 152 and secondary needle tubing 151, by slip of the secondary plunger 152 in secondary needle tubing 151 by secondary pin
Tumor tissues in pipe 151 are expressed in main needle tubing 11.
In the present embodiment, two secondary needle tubings 151 are connected on main needle tubing 11 side by side, and secondary needle tubing 151 axis and main pin
The axis of pipe 11 is vertical.It is arranged so as to be advantageous in that, outward appearance neat appearance.And do not extruded in secondary plunger 152, tumour therein
Tissue will not be produced from flowing because of gravity, so that inoculation 15 pairs of injection rates of the tumor tissues of main needle tubing 11 of secondary pin
Control it is more accurate.
Secondary needle tubing 151 is marked with volume markings, and each scale is 0.1ml, it is adaptable to the inoculation of micro microorganism.Indicate
The secondary needle tubing 151 of volume markings causes the secondary pin 15 of inoculation, directly perceived to the injection rate of the tumor tissues of main needle tubing 11 and controllable so that
Microbial inoculant pin 1 becomes in order to one kind is quantitative, continuous transfer needle.
By being detachably connected between secondary needle tubing 151 and main needle tubing 11, in the present embodiment, secondary needle tubing 151 and main needle tubing 11
It is connected through a screw thread, the interface 111 on main needle tubing 11 is provided with internal thread, secondary needle tubing 151 is provided with what is be engaged with internal thread
External screw thread.It is screwed on interface 111 by by secondary needle tubing 151, so as to realize being detachably connected for main needle tubing 11 and secondary needle tubing 151.
By the way of threaded connection, simple structure, it is convenient to mount and dismount, connect reliable, while threaded connection is with more excellent sealing
Property.
It is the sealing being further ensured that between main needle tubing 11 and secondary needle tubing 151, sealing ring is additionally provided with interface 111, when
During to being screwed into secondary needle tubing 151 on interface 111, secondary needle tubing 151 says that sealing ring is compressed, and sealing ring occurs elastic deformation, supervenes
Gap between main needle tubing 11 and secondary needle tubing 151 is filled and compressed by one elastic-restoring force, the elastic-restoring force, so as to reach
To the effect of sealing.It is appreciated that being sealed into end face seal between main needle tubing 11 and secondary needle tubing 151.
In the present embodiment, the secondary pin 15 of inoculation is stainless steel, and cleaning and sterilizing is carried out after use can Reusability.To connect
Plant secondary pin 15 and pulled down from main needle tubing 11 and load tumor tissues thereto, the whole end face of secondary needle tubing 151 is opening, tumor tissues
Loading it is more convenient.By scale marker and secondary plunger 152 to injection tumor tissues quantitative in main needle tubing 11.
Specifically, being to the method for mouse inoculation tumor tissues：
1. tumor tissues fritter is fitted into the secondary needle tubing 151 of the secondary pin 15 of inoculation；
2. secondary needle tubing 151 is threadedly attached on main needle tubing 11；
3. the tumor tissues fritter of 0.1-0.2ml is released by secondary plunger 152；
4. the lowermost end that plug 13 shifts main needle tubing 11 onto will be promoted mainly；
5. syringe needle 12 is penetrated from mouse side subcutaneous abdomen, walked to oxter；
6. will push pin and 14 shift bottom onto.
By above-mentioned steps, complete to a tumor tissues inoculation for mouse, when being inoculated with to next mouse,
Need to repeat steps 3-6, tumor tissues are taken without filling again, continuously multiple mouse can be inoculated with.
Embodiment 2
As shown in figure 3, the present embodiment is with the difference of embodiment 1, in the present embodiment, one is connected with main needle tubing 11
The secondary pin 15 of inoculation.One secondary pin 15 of inoculation is meet can be on the premise of vaccinization, and for main needle tubing 11 leaves substantial amounts of holding
Hold space so that the gripping of main needle tubing 11 is more comfortable.
The present embodiment is also resided in the difference of embodiment 1, and in the present embodiment, main needle tubing 11 passes through clamping with secondary needle tubing 151
Connection, specifically, interface 111 is provided with projection, secondary needle tubing 151 is provided with annular groove, by matching somebody with somebody for raised and annular groove
Close and cause that secondary needle tubing 151 is connected on main needle tubing 11.The clamping connection of main needle tubing 11 and secondary needle tubing 151 makes attachment structure very simple
It is single, it is capable of achieving by plug in assembly and disassembly.Main needle tubing 11 and the secondary support of needle tubing 151 are additionally provided with sealing ring, close in the present embodiment
Seal is placed on the cylinder of secondary needle tubing 151, and forming cylinder with interface 111 seals.
Embodiment 3
As shown in figure 4, the present embodiment is with the difference of embodiment 1, in the present embodiment, three are connected with main needle tubing 11
Secondary transfer needle.Three secondary needle tubings 151 are distributed side by side on main needle tubing 11, finger can be ridden over into secondary pin when main needle tubing 11 is gripped
On pipe 151, the comfort level for gripping is not influenceed, while cause that the total capacity of secondary needle tubing 151 is bigger again, can be simultaneously to more mouse
It is inoculated with.
The present embodiment is also resided in the difference of embodiment 1, in the present embodiment, for not between secondary needle tubing 151 and main needle tubing 11
It is detachably connected, the part that specific main needle tubing 11 is formed in one with secondary needle tubing 151, between main needle tubing 11 and secondary needle tubing 151
Sealing it is good.When dress takes tumor tissues, by secondary plunger 152 from the extraction in secondary needle tubing 151, from the opening to secondary needle tubing
Tumor tissues are added in 151.
Microbial inoculant needle set of the invention has the advantages that：
1. microbial inoculant pin of the invention is the form of trocar in main needle tubing, and the secondary pin of inoculation is added on main needle tubing, will
Microorganism is stored in secondary needle tubing, is on the one hand easy to microorganism is added in secondary needle tubing, on the other hand can be deposited in the secondary pin of inoculation
Store up a certain amount of microorganism, can be repeatedly inoculated with, without repeatedly, it is troublesome dress take microorganism, be that one kind can be continuous
Be inoculated with, microorganism dress takes convenient, time saving and energy saving, inoculation efficiency microbial inoculant pin high.
2. it is inoculated with the secondary needle tubing of secondary pin and is labeled with volume markings, can makes to be noted by the secondary pin of inoculation by the structure of trocar
The microorganism for entering all releases from syringe needle, thus the microbial biomass injected in the secondary pin of inoculation is inoculum concentration, is that one kind can determine
Amount injection, the microbial inoculant pin that effect of inoculation is good.
3. being detachably connected between secondary pin and main needle tubing is inoculated with so that the dress extract operation of microorganism is easier, and dress takes
It is in hgher efficiency.
In all examples being illustrated and described herein, any occurrence should be construed as merely exemplary, without
Be as limitation, therefore, other examples of exemplary embodiment can have different values.
It should be noted that：Similar label and letter represents similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined in individual accompanying drawing, then it need not be further defined and explained in subsequent accompanying drawing.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
Enclose.
Claims (10)
1. microbial inoculant pin, including main needle tubing, the syringe needle being connected on the main needle tubing, slidably in the main needle tubing
Promote mainly plug, it is characterised in that
Also include pushing pin and being inoculated with secondary pin, described pushing pin slidably is promoted mainly in Sai Nei and the syringe needle located at described；
Secondary pin is inoculated with described at least one on the main needle tubing, the secondary pin of inoculation includes what is connected with the main needle tubing
The secondary needle tubing of inoculation and the slidably secondary plunger in the secondary needle tubing of the inoculation.
2. microbial inoculant pin according to claim 1, it is characterised in that the length for promoting mainly plug is not less than the master
The length of needle tubing；
The length pushed pin is more than the length sum for promoting mainly plug and the syringe needle.
3. microbial inoculant pin according to claim 2, it is characterised in that described pushing pin is provided with limiting section；
The limiting section with it is described promote mainly plug and abut when, it is described push pin from it is described promote mainly plug in the length stretched out more than the syringe needle
Length.
4. microbial inoculant pin according to claim 3, it is characterised in that the plug of promoting mainly is described hollow for hollow tube
Hole be engaged with described pushing pin；
The limiting section is the projection for being provided close to the end of pushing pin, and is pushed pin described in the raised limitation and is promoted mainly in plug to described
Slide.
5. the microbial inoculant pin according to claim any one of 1-4, it is characterised in that each secondary needle tubing is marked with
Volume markings.
6. the microbial inoculant pin according to claim any one of 1-4, it is characterised in that each secondary needle tubing with it is described
Main needle tubing is detachable.
7. microbial inoculant pin according to claim 6, it is characterised in that the secondary needle tubing passes through spiral shell with the main needle tubing
Line is connected.
8. microbial inoculant pin according to claim 6, it is characterised in that the secondary needle tubing is with the main needle tubing by card
Connect in succession.
9. the microbial inoculant pin according to claim any one of 1-4, it is characterised in that the endoporus of the syringe needle with it is described
Push pin and be engaged.
10. the microbial inoculant pin according to claim any one of 1-4, it is characterised in that the syringe needle and the main pin
Pipe is detachable.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611231529.XA CN106754299B (en) | 2016-12-28 | 2016-12-28 | Tumor tissue inoculating needle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231529.XA CN106754299B (en) | 2016-12-28 | 2016-12-28 | Tumor tissue inoculating needle |
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| CN106754299A true CN106754299A (en) | 2017-05-31 |
| CN106754299B CN106754299B (en) | 2023-07-25 |
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| CN201611231529.XA Active CN106754299B (en) | 2016-12-28 | 2016-12-28 | Tumor tissue inoculating needle |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592753A (en) * | 2018-06-12 | 2019-12-20 | 尚科纺织企业工业及贸易公司 | Method of providing a microbial culture to an elongate element |
| CN118649003A (en) * | 2024-08-22 | 2024-09-17 | 上海雏田生物技术有限公司 | Tumor tissue inoculating needle |
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