CN106720301A - A kind of pasteurization milk and preparation method thereof - Google Patents
A kind of pasteurization milk and preparation method thereof Download PDFInfo
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- CN106720301A CN106720301A CN201611031141.5A CN201611031141A CN106720301A CN 106720301 A CN106720301 A CN 106720301A CN 201611031141 A CN201611031141 A CN 201611031141A CN 106720301 A CN106720301 A CN 106720301A
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- milk
- pasteurization
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- lactobacillus plantarum
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- 235000013336 milk Nutrition 0.000 title claims abstract description 51
- 239000008267 milk Substances 0.000 title claims abstract description 51
- 210000004080 milk Anatomy 0.000 title claims abstract description 51
- 238000009928 pasteurization Methods 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 241001462463 Lactobacillus plantarum ST-III Species 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims description 14
- 235000020185 raw untreated milk Nutrition 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 235000020183 skimmed milk Nutrition 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 239000012467 final product Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 2
- 235000020122 reconstituted milk Nutrition 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- 238000012549 training Methods 0.000 claims 1
- 235000020247 cow milk Nutrition 0.000 abstract description 8
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 230000000968 intestinal effect Effects 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 240000006024 Lactobacillus plantarum Species 0.000 abstract description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000006041 probiotic Substances 0.000 description 22
- 235000018291 probiotics Nutrition 0.000 description 22
- 238000003860 storage Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 7
- 230000006870 function Effects 0.000 description 5
- 238000005057 refrigeration Methods 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 238000011049 filling Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 235000020167 acidified milk Nutrition 0.000 description 3
- 210000000481 breast Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013406 prebiotics Nutrition 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000001139 pH measurement Methods 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002058 anti-hyperglycaemic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000033083 heart process Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000020200 pasteurised milk Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of pasteurization milk and preparation method thereof, wherein the pasteurization milk includes Lactobacillus plantarum ST III, and its addition viable count in pasteurization milk is not less than 1 × 107CFU/mL.Be added to Lactobacillus plantarum with beneficial function in cow's milk to prepare pasteurization milk by the present invention, can improve product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, immunity of organisms be improved, with promotional value higher.
Description
Technical field
The present invention relates to dairy fermentation technical field, and in particular to a kind of bus sterilization breast and preparation method thereof, more specifically
It is related to a kind of bus sterilization breast containing Lactobacillus plantarum ST-III and preparation method thereof.
Background technology
Probiotics refers to the microorganism of the work for reaching a number of, to act on host's unsoundness.Play probiotics
Prebiotic effect, it is necessary to assure daily intake sufficient amount of active bacteria, absorption mode is relatively conventional by carrier of dairy products.When
Preceding in the market probiotic yogurt species is various, vdiverse in function, and consumer's range of choice is wider.Add probiotics commonly at present
It is without probiotics in acidified milk or Yoghourt, in pasteurized milk.And most probiotics energy in milk
Growth;Growth and breeding can produce acid in milk, cause taste difference.Further, for often drinking fresh milk or pasteurize milk
Consumer groups, then extra intake probiotic yogurt meets body and enteron aisle demand, substantially increases consumption pressure, if by probiotics
Effectively it is combined together with fresh milk or pasteurize milk, pole is significantly met the demand of this kind of crowd, at present city of the country
Almost there be not such product, therefore pasteurize milk of the exploitation containing probiotics has certain necessity, is both to prebiotic on field
Perfect, raising product quality and the nutrition added valve of becteriums product, can also help consumer's regulating intestinal canal balance, raising body to exempt from
Epidemic disease power.
It has been reported that Lactobacillus plantarum ST-III has in Chinese invention patent ZL03116377.7, ZL200410066891.7
There are antihyperglycemic, reducing blood lipid, reduce the functions such as cholesterol, adhesion intestinal epithelial cell, beneficial function is notable.While plant breast bar
Bacterium ST-III is also domestic first open bacterial strain reported and carry out industrialization, its application value is carried out into expansion and is pushed away, for the country
The development of dairy industry is significant.
The content of the invention
For prior art blank, the present invention provides a kind of pasteurization milk, by the Lactobacillus plantarum with beneficial function
ST-III is added in cow's milk to prepare pasteurization milk, and ST-III hardly grows in cow's milk and produces acid, will not cause mouth
Taste difference, and the probiotics ST-III of viable count high has norcholesterol, reducing blood lipid, adhesion intestinal epithelial cell and fat-reducing etc.
Effect, can improve product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, improve immunity of organisms.
The preparation method of above-mentioned pasteurization milk is also disclosed, is added by using Lactobacillus plantarum ST-III as probiotics
Obtained into pasteurization milk, method is simple, it is easy to grasp, and nonnutritive loss.
To achieve the above object, the present invention is adopted the following technical scheme that:
A kind of pasteurization milk, the pasteurization milk includes Lactobacillus plantarum ST-III.
Preferably, the addition of Lactobacillus plantarum ST-III viable count in pasteurization milk be not less than 1 ×
107CFU/mL。
The preparation method of above-mentioned pasteurization milk, comprises the following steps:
(1) homogeneous, pasteurize are carried out to raw milk, in cooling under aseptic condition;
(2) pure culture of Lactobacillus plantarum ST-III is aseptically seeded to the raw milk described in step (1)
In, it is stored refrigerated to obtain final product.
Wherein, the raw milk be in raw milk, skimmed milk, reconstituted milk and Soybean Milk any one is several.More preferably
It, selected from any one in raw milk and skimmed milk, is most preferably raw milk that ground is.
The Parameter Conditions of the homogeneous that step (1) is used are preferably 60-65 DEG C, 18-20MPa.
The pasteurize condition for being used for:The sterilization processing 30min under 63 DEG C of temperature conditionss, or killed under the conditions of 72 DEG C
Bacterium processes the 15s or sterilization processing 0.5s under the conditions of 90 DEG C, and three can reach preferable treatment effect.
Preferably, before pasteurize is carried out, raw milk can be standardized.
Preferably, the refrigerated storage temperature is maintained at 4-10 DEG C.
Wherein, the invention further particularly discloses above-mentioned use Lactobacillus plantarum ST-III pure cultures preparation method
For:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with body
Product is seeded on MRS culture mediums than the ratio for 2%, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:After step (1) gained nutrient solution is carried out into centrifugal treating, take
Sediment pH is that 7.0 PBS is rinsed 2 times, adds skimmed milk freeze drying protectant, and adjustment cell concentration is to 109cfu/
ML, vacuum freeze drying is carried out after being well mixed, and the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
In step (2), the condition of the centrifugal treating is:Temperature be 4 DEG C, rotating speed be 4000rpm under conditions of from
The heart processes 10min.
On the basis of common sense in the field is met, above-mentioned each optimum condition can be combined, and obtain final product each good reality of the present invention
Example.
Above-mentioned acidified milk can be prepared into various fermented dairy products according to actual conditions, every to utilize acidified milk of the invention
The dairy products of preparation, are within the scope of the present invention.
Beneficial effects of the present invention are:
Be added to Lactobacillus plantarum with beneficial function in cow's milk to prepare pasteurization milk by the present invention, can improve
Product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, improve immunity of organisms.
Most of probiotics growth and breeding in milk can produce acid, cause taste difference.And Lactobacillus plantarum ST-III exists
Hardly grown in cow's milk and produce acid, taste difference will not be caused.And probiotics contains in pasteurization milk prepared by the present invention
Amount is high, has facilitation to human body intestinal canal health after intake.
Specific embodiment
The present invention will be further explained by specific embodiment below, it being understood, however, that can be with each
The form of kind realizes the present invention without that should be limited by embodiments set forth here.Conversely, there is provided these embodiments are able to
Be best understood from the present invention, and can by the scope of the present invention it is complete convey to those skilled in the art.
As the "comprising" in specification in the whole text and claim mentioned in or " including " be an open language, therefore should
It is construed to " include but be not limited to ".To implement better embodiment of the invention, so description is specification subsequent descriptions
For the purpose of the rule of specification, the scope of the present invention is not limited to.Protection scope of the present invention is when regarding appended power
Profit requires that the person of defining is defined.
Such as without specified otherwise, various raw materials of the present invention are commercially available.
The preparation of the Lactobacillus plantarum ST-III pure cultures of embodiment 1
Comprise the following steps that:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with body
Product is seeded on MRS culture mediums than the ratio for 2%, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:Step (1) gained nutrient solution is carried out into centrifugal treating (in temperature
It is 4 DEG C to spend, and rotating speed is centrifugal treating 10min under conditions of 4000rpm) after, taking precipitate pH is 7.0 PBS punching
Wash 2 times, add skimmed milk freeze drying protectant, adjustment cell concentration to 109Cfu/mL, carries out vacuum refrigeration and does after being well mixed
It is dry, the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
A kind of preparation of the pasteurization milk of embodiment 2
(1) raw milk 1000.0g is weighed, in 65 DEG C, homogenization under the conditions of 18MPa, the then Pasteur under the conditions of 72 DEG C
Sterilized 15s, is cooled to 4-10 DEG C.
(2) the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III prepared according to the method for embodiment 1 are weighed and adds to step (1)
In gained cow's milk, stir, in the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 3
(1) skimmed milk 1000.0g is weighed, under the conditions of 65 DEG C, pasteurize 30min is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained skimmed milk, stir,
In the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 4
(1) commercially available fresh cow milk 1000.0g, in 60 DEG C, homogenization under the conditions of 20MPa are weighed.Then under the conditions of 90 DEG C
Pasteurize 0.5s, is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained cow's milk, stir, nothing
In the filling container to sterilizing of bacterium condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 5
(1) Soybean Milk 1000.0g is weighed, under the conditions of 65 DEG C, pasteurize 30min is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained Soybean Milk, stir,
In the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
The pasteurization milk that applicant's selection embodiment 2 is prepared carries out index determining, including pH value, titratable acidity, benefit
Raw bacterial content is determined.
It is specific as follows:
Using pH value in pH meter (Switzerland METTLER) measure storage;
The change of titratable acidity in storage is determined using GB 5413.34-2010 methods;
The content of probiotics in storage is determined using GB 4789.35-2010 methods.
Wherein blank is the change for being not added with prebiotic fungus lactobacillus ST-III.
Wherein, probiotics pasteurization milk during probiotics pasteurization milk pH measurement results are shown in Table 1, storage during storage
Probiotics pasteurization milk probiotics assay the results are shown in Table 3 during titratable acidity measurement result is shown in Table 2, storage.
Probiotics pasteurization milk pH measurement results during the storage of table 1
Probiotics pasteurization milk titratable acidity measurement result during the storage of table 2
Probiotics pasteurization milk probiotics assay result during the storage of table 3
From the comparing result of table 1 and 2, the addition of Lactobacillus plantarum ST-III is not to the physical and chemical index of pasteurization milk
Impact, and from table 3 it can be seen that viable count is held essentially constant within the shelf-life.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Any modification, equivalent and simple modifications for being made in content etc., should be included within the scope of the present invention.
Claims (9)
1. a kind of pasteurization milk, it is characterised in that the pasteurization milk includes Lactobacillus plantarum ST-III.
2. pasteurization milk according to claim 1, it is characterised in that the addition of the Lactobacillus plantarum ST-III exists
Viable count is not less than 1 × 10 in pasteurization milk7CFU/mL。
3. a kind of preparation method of pasteurization milk according to claim 1 and 2, it is characterised in that the preparation method
Comprise the following steps:
(1) homogeneous, pasteurize are carried out to raw milk, in cooling under aseptic condition;
(2) aseptically the pure culture of Lactobacillus plantarum ST-III is seeded in the raw milk described in step (1), it is cold
Preservation is hidden to obtain final product.
4. the preparation method of pasteurization milk according to claim 3, it is characterised in that the raw milk be raw milk,
Any one in skimmed milk, reconstituted milk and Soybean Milk is several.
5. the preparation method of pasteurization milk according to claim 4, it is characterised in that the raw milk is raw milk.
6. the preparation method of pasteurization milk according to claim 3, it is characterised in that the Parameter Conditions of homogeneous are 60-
65 DEG C, 18-20MPa.
7. the preparation method of pasteurization milk according to claim 3, it is characterised in that the pasteurize condition for being used
For:The sterilization processing 30min under 63 DEG C of temperature conditionss, or sterilization processing 15s or the sterilization under the conditions of 90 DEG C under the conditions of 72 DEG C
Treatment 0.5s.
8. the preparation method of pasteurization milk according to claim 3, it is characterised in that the pure trainings of Lactobacillus plantarum ST-III
Support thing preparation method be:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with volume ratio
Ratio for 2% is seeded on MRS culture mediums, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:After step (1) gained nutrient solution is carried out into centrifugal treating, precipitation is taken
Thing pH is that 7.0 PBS is rinsed 2 times, adds skimmed milk freeze drying protectant, and adjustment cell concentration is to 109Cfu/mL, mixes
Vacuum freeze drying is carried out after closing uniformly, the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
9. the preparation method of pasteurization milk according to claim 8, it is characterised in that the condition of the centrifugal treating
For:Temperature be 4 DEG C, rotating speed be 4000rpm under conditions of centrifugal treating 10min.
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CN201611031141.5A CN106720301A (en) | 2016-11-17 | 2016-11-17 | A kind of pasteurization milk and preparation method thereof |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113632839A (en) * | 2021-07-20 | 2021-11-12 | 卡士乳业(深圳)有限公司 | Pasteurized milk containing probiotics and preparation method thereof |
CN115736024A (en) * | 2022-12-07 | 2023-03-07 | 光明乳业股份有限公司 | Probiotic-containing oat beverage and preparation method thereof |
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CN1754545A (en) * | 2004-09-29 | 2006-04-05 | 光明乳业股份有限公司 | Use of plant lactobacillus ST-III in fat-reduction |
CN103120208A (en) * | 2011-11-18 | 2013-05-29 | 光明乳业股份有限公司 | Lactobacillus plantarum fermented milk and preparation method thereof |
CN104663884A (en) * | 2014-12-11 | 2015-06-03 | 内蒙古农业大学 | Pasteurized milk containing probiotics and preparation method of pasteurized milk |
-
2016
- 2016-11-17 CN CN201611031141.5A patent/CN106720301A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1754545A (en) * | 2004-09-29 | 2006-04-05 | 光明乳业股份有限公司 | Use of plant lactobacillus ST-III in fat-reduction |
CN103120208A (en) * | 2011-11-18 | 2013-05-29 | 光明乳业股份有限公司 | Lactobacillus plantarum fermented milk and preparation method thereof |
CN104663884A (en) * | 2014-12-11 | 2015-06-03 | 内蒙古农业大学 | Pasteurized milk containing probiotics and preparation method of pasteurized milk |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113632839A (en) * | 2021-07-20 | 2021-11-12 | 卡士乳业(深圳)有限公司 | Pasteurized milk containing probiotics and preparation method thereof |
CN113632839B (en) * | 2021-07-20 | 2024-09-10 | 卡士乳业(深圳)有限公司 | Pasteurized milk containing probiotics and preparation method thereof |
CN115736024A (en) * | 2022-12-07 | 2023-03-07 | 光明乳业股份有限公司 | Probiotic-containing oat beverage and preparation method thereof |
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