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CN106720301A - A kind of pasteurization milk and preparation method thereof - Google Patents

A kind of pasteurization milk and preparation method thereof Download PDF

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Publication number
CN106720301A
CN106720301A CN201611031141.5A CN201611031141A CN106720301A CN 106720301 A CN106720301 A CN 106720301A CN 201611031141 A CN201611031141 A CN 201611031141A CN 106720301 A CN106720301 A CN 106720301A
Authority
CN
China
Prior art keywords
milk
pasteurization
iii
preparation
lactobacillus plantarum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611031141.5A
Other languages
Chinese (zh)
Inventor
洪青
杭锋
穆海菠
雍靖怡
刘振民
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Bright Dairy and Food Co Ltd
Bright Dairy and Food Co Ltd
Original Assignee
Shanghai Bright Dairy and Food Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Bright Dairy and Food Co Ltd filed Critical Shanghai Bright Dairy and Food Co Ltd
Priority to CN201611031141.5A priority Critical patent/CN106720301A/en
Publication of CN106720301A publication Critical patent/CN106720301A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of pasteurization milk and preparation method thereof, wherein the pasteurization milk includes Lactobacillus plantarum ST III, and its addition viable count in pasteurization milk is not less than 1 × 107CFU/mL.Be added to Lactobacillus plantarum with beneficial function in cow's milk to prepare pasteurization milk by the present invention, can improve product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, immunity of organisms be improved, with promotional value higher.

Description

A kind of pasteurization milk and preparation method thereof
Technical field
The present invention relates to dairy fermentation technical field, and in particular to a kind of bus sterilization breast and preparation method thereof, more specifically It is related to a kind of bus sterilization breast containing Lactobacillus plantarum ST-III and preparation method thereof.
Background technology
Probiotics refers to the microorganism of the work for reaching a number of, to act on host's unsoundness.Play probiotics Prebiotic effect, it is necessary to assure daily intake sufficient amount of active bacteria, absorption mode is relatively conventional by carrier of dairy products.When Preceding in the market probiotic yogurt species is various, vdiverse in function, and consumer's range of choice is wider.Add probiotics commonly at present It is without probiotics in acidified milk or Yoghourt, in pasteurized milk.And most probiotics energy in milk Growth;Growth and breeding can produce acid in milk, cause taste difference.Further, for often drinking fresh milk or pasteurize milk Consumer groups, then extra intake probiotic yogurt meets body and enteron aisle demand, substantially increases consumption pressure, if by probiotics Effectively it is combined together with fresh milk or pasteurize milk, pole is significantly met the demand of this kind of crowd, at present city of the country Almost there be not such product, therefore pasteurize milk of the exploitation containing probiotics has certain necessity, is both to prebiotic on field Perfect, raising product quality and the nutrition added valve of becteriums product, can also help consumer's regulating intestinal canal balance, raising body to exempt from Epidemic disease power.
It has been reported that Lactobacillus plantarum ST-III has in Chinese invention patent ZL03116377.7, ZL200410066891.7 There are antihyperglycemic, reducing blood lipid, reduce the functions such as cholesterol, adhesion intestinal epithelial cell, beneficial function is notable.While plant breast bar Bacterium ST-III is also domestic first open bacterial strain reported and carry out industrialization, its application value is carried out into expansion and is pushed away, for the country The development of dairy industry is significant.
The content of the invention
For prior art blank, the present invention provides a kind of pasteurization milk, by the Lactobacillus plantarum with beneficial function ST-III is added in cow's milk to prepare pasteurization milk, and ST-III hardly grows in cow's milk and produces acid, will not cause mouth Taste difference, and the probiotics ST-III of viable count high has norcholesterol, reducing blood lipid, adhesion intestinal epithelial cell and fat-reducing etc. Effect, can improve product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, improve immunity of organisms.
The preparation method of above-mentioned pasteurization milk is also disclosed, is added by using Lactobacillus plantarum ST-III as probiotics Obtained into pasteurization milk, method is simple, it is easy to grasp, and nonnutritive loss.
To achieve the above object, the present invention is adopted the following technical scheme that:
A kind of pasteurization milk, the pasteurization milk includes Lactobacillus plantarum ST-III.
Preferably, the addition of Lactobacillus plantarum ST-III viable count in pasteurization milk be not less than 1 × 107CFU/mL。
The preparation method of above-mentioned pasteurization milk, comprises the following steps:
(1) homogeneous, pasteurize are carried out to raw milk, in cooling under aseptic condition;
(2) pure culture of Lactobacillus plantarum ST-III is aseptically seeded to the raw milk described in step (1) In, it is stored refrigerated to obtain final product.
Wherein, the raw milk be in raw milk, skimmed milk, reconstituted milk and Soybean Milk any one is several.More preferably It, selected from any one in raw milk and skimmed milk, is most preferably raw milk that ground is.
The Parameter Conditions of the homogeneous that step (1) is used are preferably 60-65 DEG C, 18-20MPa.
The pasteurize condition for being used for:The sterilization processing 30min under 63 DEG C of temperature conditionss, or killed under the conditions of 72 DEG C Bacterium processes the 15s or sterilization processing 0.5s under the conditions of 90 DEG C, and three can reach preferable treatment effect.
Preferably, before pasteurize is carried out, raw milk can be standardized.
Preferably, the refrigerated storage temperature is maintained at 4-10 DEG C.
Wherein, the invention further particularly discloses above-mentioned use Lactobacillus plantarum ST-III pure cultures preparation method For:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with body Product is seeded on MRS culture mediums than the ratio for 2%, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:After step (1) gained nutrient solution is carried out into centrifugal treating, take Sediment pH is that 7.0 PBS is rinsed 2 times, adds skimmed milk freeze drying protectant, and adjustment cell concentration is to 109cfu/ ML, vacuum freeze drying is carried out after being well mixed, and the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
In step (2), the condition of the centrifugal treating is:Temperature be 4 DEG C, rotating speed be 4000rpm under conditions of from The heart processes 10min.
On the basis of common sense in the field is met, above-mentioned each optimum condition can be combined, and obtain final product each good reality of the present invention Example.
Above-mentioned acidified milk can be prepared into various fermented dairy products according to actual conditions, every to utilize acidified milk of the invention The dairy products of preparation, are within the scope of the present invention.
Beneficial effects of the present invention are:
Be added to Lactobacillus plantarum with beneficial function in cow's milk to prepare pasteurization milk by the present invention, can improve Product quality and nutrition added valve, can also help consumer's regulating intestinal canal to balance, improve immunity of organisms.
Most of probiotics growth and breeding in milk can produce acid, cause taste difference.And Lactobacillus plantarum ST-III exists Hardly grown in cow's milk and produce acid, taste difference will not be caused.And probiotics contains in pasteurization milk prepared by the present invention Amount is high, has facilitation to human body intestinal canal health after intake.
Specific embodiment
The present invention will be further explained by specific embodiment below, it being understood, however, that can be with each The form of kind realizes the present invention without that should be limited by embodiments set forth here.Conversely, there is provided these embodiments are able to Be best understood from the present invention, and can by the scope of the present invention it is complete convey to those skilled in the art.
As the "comprising" in specification in the whole text and claim mentioned in or " including " be an open language, therefore should It is construed to " include but be not limited to ".To implement better embodiment of the invention, so description is specification subsequent descriptions For the purpose of the rule of specification, the scope of the present invention is not limited to.Protection scope of the present invention is when regarding appended power Profit requires that the person of defining is defined.
Such as without specified otherwise, various raw materials of the present invention are commercially available.
The preparation of the Lactobacillus plantarum ST-III pure cultures of embodiment 1
Comprise the following steps that:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with body Product is seeded on MRS culture mediums than the ratio for 2%, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:Step (1) gained nutrient solution is carried out into centrifugal treating (in temperature It is 4 DEG C to spend, and rotating speed is centrifugal treating 10min under conditions of 4000rpm) after, taking precipitate pH is 7.0 PBS punching Wash 2 times, add skimmed milk freeze drying protectant, adjustment cell concentration to 109Cfu/mL, carries out vacuum refrigeration and does after being well mixed It is dry, the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
A kind of preparation of the pasteurization milk of embodiment 2
(1) raw milk 1000.0g is weighed, in 65 DEG C, homogenization under the conditions of 18MPa, the then Pasteur under the conditions of 72 DEG C Sterilized 15s, is cooled to 4-10 DEG C.
(2) the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III prepared according to the method for embodiment 1 are weighed and adds to step (1) In gained cow's milk, stir, in the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 3
(1) skimmed milk 1000.0g is weighed, under the conditions of 65 DEG C, pasteurize 30min is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained skimmed milk, stir, In the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 4
(1) commercially available fresh cow milk 1000.0g, in 60 DEG C, homogenization under the conditions of 20MPa are weighed.Then under the conditions of 90 DEG C Pasteurize 0.5s, is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained cow's milk, stir, nothing In the filling container to sterilizing of bacterium condition, 4 DEG C of refrigerations.
A kind of preparation of the pasteurization milk of embodiment 5
(1) Soybean Milk 1000.0g is weighed, under the conditions of 65 DEG C, pasteurize 30min is cooled to 4-10 DEG C.
(2) weigh during the lyophilized bacterium powder 0.05g of Lactobacillus plantarum ST-III add to step (1) gained Soybean Milk, stir, In the filling container to sterilizing of aseptic condition, 4 DEG C of refrigerations.
The pasteurization milk that applicant's selection embodiment 2 is prepared carries out index determining, including pH value, titratable acidity, benefit Raw bacterial content is determined.
It is specific as follows:
Using pH value in pH meter (Switzerland METTLER) measure storage;
The change of titratable acidity in storage is determined using GB 5413.34-2010 methods;
The content of probiotics in storage is determined using GB 4789.35-2010 methods.
Wherein blank is the change for being not added with prebiotic fungus lactobacillus ST-III.
Wherein, probiotics pasteurization milk during probiotics pasteurization milk pH measurement results are shown in Table 1, storage during storage Probiotics pasteurization milk probiotics assay the results are shown in Table 3 during titratable acidity measurement result is shown in Table 2, storage.
Probiotics pasteurization milk pH measurement results during the storage of table 1
Probiotics pasteurization milk titratable acidity measurement result during the storage of table 2
Probiotics pasteurization milk probiotics assay result during the storage of table 3
From the comparing result of table 1 and 2, the addition of Lactobacillus plantarum ST-III is not to the physical and chemical index of pasteurization milk Impact, and from table 3 it can be seen that viable count is held essentially constant within the shelf-life.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention Any modification, equivalent and simple modifications for being made in content etc., should be included within the scope of the present invention.

Claims (9)

1. a kind of pasteurization milk, it is characterised in that the pasteurization milk includes Lactobacillus plantarum ST-III.
2. pasteurization milk according to claim 1, it is characterised in that the addition of the Lactobacillus plantarum ST-III exists Viable count is not less than 1 × 10 in pasteurization milk7CFU/mL。
3. a kind of preparation method of pasteurization milk according to claim 1 and 2, it is characterised in that the preparation method Comprise the following steps:
(1) homogeneous, pasteurize are carried out to raw milk, in cooling under aseptic condition;
(2) aseptically the pure culture of Lactobacillus plantarum ST-III is seeded in the raw milk described in step (1), it is cold Preservation is hidden to obtain final product.
4. the preparation method of pasteurization milk according to claim 3, it is characterised in that the raw milk be raw milk, Any one in skimmed milk, reconstituted milk and Soybean Milk is several.
5. the preparation method of pasteurization milk according to claim 4, it is characterised in that the raw milk is raw milk.
6. the preparation method of pasteurization milk according to claim 3, it is characterised in that the Parameter Conditions of homogeneous are 60- 65 DEG C, 18-20MPa.
7. the preparation method of pasteurization milk according to claim 3, it is characterised in that the pasteurize condition for being used For:The sterilization processing 30min under 63 DEG C of temperature conditionss, or sterilization processing 15s or the sterilization under the conditions of 90 DEG C under the conditions of 72 DEG C Treatment 0.5s.
8. the preparation method of pasteurization milk according to claim 3, it is characterised in that the pure trainings of Lactobacillus plantarum ST-III Support thing preparation method be:
(1) Lactobacillus plantarum ST-III spreads cultivation:Under aseptic technique by Lactobacillus plantarum ST-III seed liquors with volume ratio Ratio for 2% is seeded on MRS culture mediums, and quiescent culture 18h obtains nutrient solution under 37 DEG C of temperature conditionss;
(2) prepared by the lyophilized bacterium powders of Lactobacillus plantarum ST-III:After step (1) gained nutrient solution is carried out into centrifugal treating, precipitation is taken Thing pH is that 7.0 PBS is rinsed 2 times, adds skimmed milk freeze drying protectant, and adjustment cell concentration is to 109Cfu/mL, mixes Vacuum freeze drying is carried out after closing uniformly, the lyophilized bacterium powders of described Lactobacillus plantarum ST-III are obtained final product after freezing.
9. the preparation method of pasteurization milk according to claim 8, it is characterised in that the condition of the centrifugal treating For:Temperature be 4 DEG C, rotating speed be 4000rpm under conditions of centrifugal treating 10min.
CN201611031141.5A 2016-11-17 2016-11-17 A kind of pasteurization milk and preparation method thereof Pending CN106720301A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611031141.5A CN106720301A (en) 2016-11-17 2016-11-17 A kind of pasteurization milk and preparation method thereof

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113632839A (en) * 2021-07-20 2021-11-12 卡士乳业(深圳)有限公司 Pasteurized milk containing probiotics and preparation method thereof
CN115736024A (en) * 2022-12-07 2023-03-07 光明乳业股份有限公司 Probiotic-containing oat beverage and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1754545A (en) * 2004-09-29 2006-04-05 光明乳业股份有限公司 Use of plant lactobacillus ST-III in fat-reduction
CN103120208A (en) * 2011-11-18 2013-05-29 光明乳业股份有限公司 Lactobacillus plantarum fermented milk and preparation method thereof
CN104663884A (en) * 2014-12-11 2015-06-03 内蒙古农业大学 Pasteurized milk containing probiotics and preparation method of pasteurized milk

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1754545A (en) * 2004-09-29 2006-04-05 光明乳业股份有限公司 Use of plant lactobacillus ST-III in fat-reduction
CN103120208A (en) * 2011-11-18 2013-05-29 光明乳业股份有限公司 Lactobacillus plantarum fermented milk and preparation method thereof
CN104663884A (en) * 2014-12-11 2015-06-03 内蒙古农业大学 Pasteurized milk containing probiotics and preparation method of pasteurized milk

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113632839A (en) * 2021-07-20 2021-11-12 卡士乳业(深圳)有限公司 Pasteurized milk containing probiotics and preparation method thereof
CN113632839B (en) * 2021-07-20 2024-09-10 卡士乳业(深圳)有限公司 Pasteurized milk containing probiotics and preparation method thereof
CN115736024A (en) * 2022-12-07 2023-03-07 光明乳业股份有限公司 Probiotic-containing oat beverage and preparation method thereof

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Application publication date: 20170531

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