CN106718065A - Using the method for tea tree branch cultivating black fungus - Google Patents
Using the method for tea tree branch cultivating black fungus Download PDFInfo
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- CN106718065A CN106718065A CN201611247345.2A CN201611247345A CN106718065A CN 106718065 A CN106718065 A CN 106718065A CN 201611247345 A CN201611247345 A CN 201611247345A CN 106718065 A CN106718065 A CN 106718065A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of method of utilization tea tree branch cultivating black fungus, comprise the following steps：(1) making of cultivation matrix；(2) making of edible black fungus bag；(3) edible black fungus bag sterilizing；(4) it is inoculated with；(5) cultivation；(6) ear management.Agaric fungus bags comprehensive nutrition obtained by the method for utilization tea tree branch cultivating black fungus of the invention, meets nutritional need in black fungus growth course, and the black fungus yield cultivated is high, quality better, shortens implantation time and makes full use of tea tree branch.
Description
Technical field
The present invention relates to fungus growing technique field, a kind of utilization tea tree branch cultivating black fungus are related in particular to
Method.
Background technology
Black fungus is a kind of nutritious edible mushroom, is again the traditional health food of China and exporting；It other
Name is a lot, because being grown on rotten wood, the ear of its likeness in form people, therefore named agaric.It has nutritive value higher and certain
Medical value, black fungus contains the necessary 8 kinds of Amino acids of human body and vitamin, also containing abundant colloid, is to human consumption
System has good clear and rich effect.It is a kind of saprophytic fungus, does not have chlorophyll, and oneself will not manufacture food, be by other lifes
Organic substance in object, while it seeks saprogenesis, must could grow as its nutriment on organism in the dust
Development, its mycelia is very strong to the cellulose in organism, the capacity of decomposition of hemicellulose, and organism can be made finally to crush.
The plantation of black fungus has turned into China Agricultural Structure Adjustment, peasant Tuo Ping and has got rich and ecological Export-Oriented Agriculture with cultivation
Important channel；With the continuous expansion and the development of mushroom industry of edible fungus culturing scale, substantial amounts of edible mushroom is so needed
Production matrix, Growing Edible Funguses with Substitutes technology has been widely adopted, and conventional alternative cultivation matrix is main by wood chip, wheat bran, stone
Cream powder etc. is constituted.Can just be obtained because original wood chip obtains to need to cut down a large amount of trees, cause a large amount of trees to be cut down totally,
The influence ecological balance；And oil tea tree branches and leaves are luxuriant, in order to ensure that Property of Tea oil Tree Fruit is got bumper crops, orchard worker will repair to tea oil tree every year
Cut.The annual branches and leaves resource very abundant by pruning, but existing tea tree branch is burnt mostly as firewood, causes resource
Significant wastage and serious environmental pollution；And due to the not comprehensive nutrition of traditional cultivation matrix, cause the black wood of cultivation
Ear yield is not high, and quality is also not good enough, and implantation time is more long.Conventional plantation raw material resources are fewer and feweri, it is therefore desirable to find
New raw material replace or supplement existing raw material resources.
The content of the invention
For above the deficiencies in the prior art, there is provided provide a kind of comprehensive nutrition, meet nutrition in black fungus growth course
Demand, and the black fungus yield cultivated is high, quality better, shortens implantation time and makes full use of the utilization tea tree branch of tea tree branch
The method of bar cultivating black fungus.
Realize the technical scheme is that：A kind of method of utilization tea tree branch cultivating black fungus, comprises the following steps：
(1) making of cultivation matrix：Raw material is constituted by the parts by weight of following raw material：40~50 parts of tea tree branch, bamboo willow
30~40 parts of wood chip, 10~20 parts of napier grass, 1~5 part of pulverized limestone, 1~3 part of gypsum, 5~10 parts of dregs of beans, 1~3 part of oyster shell whiting, wheat
3~6 parts of meal stone, 4~6 parts of diatomite, 2~4 parts of calcium monohydrogen phosphate, 5~8 parts of glucose, 6~10 parts of sucrose, calcium superphosphate 1~3
It is part, 0.2~0.6 part of molybdenum trisulfate, 0.1~0.6 part of phosphoric acid hydrogen ammonia, 0.1~0.8 part of copper sulphate, 0.5~0.8 part of ferrous sulfate, micro-
0.03~0.08 part of biological nano selenium；The tea tree branch from through drying to solid water content of matter be 12~13%, without mould
The tea tree branch of change is raw material, size-reduced to obtain to 60~80 mesh；Half is graininess wood chip and half in the bamboo willow bits
It is band wood sawdust；
Preparation method is：All raw materials are sufficiently mixed uniformly；Add pyrolkigneous liquid, keep moisture 50~55% it
Between；After well mixed, sealing and fermenting 8~15 days makes its abundant water swelling and softening, that is, obtain cultivation of auricularia auricula matrix；
(2) making of edible black fungus bag：During the cultivation of auricularia auricula matrix that step (1) is obtained entered into polypropylene plastics bacterium bag；
Cultivation matrix accounts for the 70%~80% of the bacterium bag inner space；Stomata is pierced in the side of bacterium bag and bottom surface；
(3) edible black fungus bag sterilizing：The bacterium bag of the loading matrix that step (2) is obtained is 120~125 DEG C, height in temperature
2~3h of pressure steam sterilizing；After end subject to sterilization, normal temperature is returned to, take out bacterium bag；
(4) it is inoculated with：Under 23~26 DEG C of gnotobasis, access is routinely cultivated, bacterium ball is uniform in size, energetic
Agaric liquid spawn, access amount is 15~25ml/ bags；And one layer of gauze soaked with limewash is covered on bacterium bag surface, connect
Kind after sending out bacterium in room temperature, shading, dry culturing room；
(5) cultivation：At 15 DEG C~24 DEG C, air humidity is maintained at 50%~60% to culturing room's temperature control, every 3~5
It carries out a miscellaneous bacteria and checks and send out the inspection of bacterium situation, timely supplement inoculation and superseded miscellaneous bacteria；When the grain of rice occurs in bacterium bag surface
During shape ear base, ventilation 2~3 times is kept daily；Continue to draw hole in bacterium bag surrounding, bacterium bag is moved into agaric growing field by culture after 5~6 days
Carry out ear management；
(6) ear management：Produce agaric temperature control widens day and night temperature control at 9 DEG C~12 DEG C at 15 DEG C~25 DEG C, empty
Gas relative humidity is maintained at 80%~95%；In good time ventilation.
Preferably, raw material is constituted by the parts by weight of following raw material：45 parts of tea tree branch, bamboo willow consider 35 parts, napier grass 15 to be worth doing
Part, 2 parts of pulverized limestone, 2 parts of gypsum, 8 parts of dregs of beans, 2 parts of oyster shell whiting, 5 parts of medical stone, 5 parts of diatomite, 3 parts of calcium monohydrogen phosphate, glucose
6 parts, 8 parts of sucrose, 2 parts of calcium superphosphate, 0.4 part of molybdenum trisulfate, 0.3 part of phosphoric acid hydrogen ammonia, 0.5 part of copper sulphate, 0.6 part of ferrous sulfate,
0.05 part of microorganism nanometer selenium.
Preferably, the preparation method of the microorganism nanometer selenium is：By selenite be prepared into 0.005mol/L~
The solution of 0.05mol/L, by bacillus licheniformis according to mixing account for selenite solution 0.1%~0.3% ratio add to
In selenite solution；And 24~48h is kept at a temperature of 28~35 DEG C, obtained using low temperature drying after crystallization.
Preferably, the band wood sawdust is well mixed with spirit of vinegar, and regulation PH is 4~6；Under the conditions of 32~38 DEG C,
Add cellulase to react 8~10h to obtain.
Preferably, the volume of the graininess wood chip is 10~18mm3.
Preferably, it is 12~15% that the napier grass is dried to moisture content for fresh napier grass；Rub broken after chopping up, adjusted with spirit of vinegar
Section PH is 4~6.
Preferably, the cellulase is in circumscribed β~dextranase, inscribe β~dextranase and β~glucuroide
One or more.
Preferably, in the step (1), the mass fraction of the spirit of vinegar is 0.5%~1%.
Preferably, in the step (1), the mass fraction of the pyrolkigneous liquid is 0.1%~0.3%.
Compared with prior art the beneficial effects of the present invention are：1st, the present invention utilizes the side of tea tree branch cultivating black fungus
In method, various raw materials especially tea tree branch, bamboo willow and napier grass abundance are cheap；Greatly reduce cost of material；
In these raw materials, bamboo willow is new hybridization of willows kind, widely used, and bamboo willow material integrated fiber content is 89.88%, wooden
Cellulose content is 21.16%；Fiber content than general trees is higher；Content of cellulose is high, is conducive to the decomposition of edible mushroom to utilize,
Fungus mycelium is sent out bacterium speed fast, napier grass in addition to content of cellulose is high, its fresh grass crude protein content 5.66%, fermented decomposition
After produce various amino acid, there is provided nitrogen source in agaric growth course；Crude fibre is after black fungus decomposition in agaric growth course
Carbon source is provided；Above-mentioned three kinds of basic substances as substrate culture, meet the demand of basal nutrient in black fungus growth course；
After collocation pulverized limestone, gypsum, dregs of beans, medical stone, diatomite and nourishing additive agent, nutrition more horn of plenty meets black fungus life
The demand of nutrient in growth process, the black fungus yield using substrate culture of the present invention is high, quality better；
2nd, the present invention in various reasonable raw material proportionings, after adding nourishing additive agent, it is nutritious；There is provided black fungus
The demand of trace element in growth course, after particularly adding microorganism nanometer selenium, microorganism nanometer selenium uses lichens gemma bar
Bacterium, available organic active selenium is converted into by inorganic selenium, is directly absorbed by agaric, antagonism poisonous metal, regulation vitamin A,
Vitamin C, vitamin E, the absorption of vitamin K and utilization, promote synthesis of protein etc., prevent agaric to harmful heavy metal unit
The absorption of element, improves the quality of agaric；Containing Se content is high, agaric that is being gone out using substrate culture of the invention, Qi Erbi in agaric
It is thick, in good taste；
3rd, manufacturing process of the present invention is relatively simple, and operation is relatively easy to；Due to carrying out cellulose degradation treatment, acid to bamboo willow
Change is processed, and makes cellulose therein be easier to be decomposed, and is absorbed beneficial to black fungus；Because fermentation process with the addition of pyrolkigneous liquid, can
Effect is obviously promoted with the growth of mycelium, fructification to black fungus, hence it is evident that improve its yield；
4th, due to reasonable nutritional arrangment of the invention, and beneficial to the decomposition and absorption of black fungus；From be seeded to harvesting when
Between compared with it is original wood in advance 7~10 days or so；
5th, in daily management, accurate adjustment is adapted to the temperature and humidity that black fungus grows each stage, it is ensured that black fungus is given birth to
Suitable temperature and humidity environment is in growth process, is conducive to produce agaric and the growth of black fungus.
6th, the present invention takes full advantage of tea tree branch, turns waste into wealth, and substantially increases planting benefit.
Specific embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below.Based on the implementation in the present invention
Example, the every other embodiment that those skilled in the art are obtained under the premise of creative work is not made belongs to this hair
The scope of bright protection.
Embodiment 1
A kind of method of utilization tea tree branch cultivating black fungus, it is characterised in that：Comprise the following steps：
(1) making of cultivation matrix：Raw material is constituted by the parts by weight of following raw material：40 parts of tea tree branch, bamboo willow bits
30 parts, 10 parts of napier grass, 1 part of pulverized limestone, 1 part of gypsum, 5 parts of dregs of beans, 1 part of oyster shell whiting, 3 parts of medical stone, 4 parts of diatomite, phosphoric acid hydrogen
2 parts of calcium, 5 parts of glucose, 6 parts of sucrose, 1 part of calcium superphosphate, 0.2 part of molybdenum trisulfate, 0.1 part of phosphoric acid hydrogen ammonia, 0.1 part of copper sulphate, sulphur
Sour ferrous iron 0.5 part, 0.03 part of microorganism nanometer selenium；Tea tree branch from through drying to solid water content of matter be 12~13%,
It is raw material without the tea tree branch that goes mouldy, it is size-reduced to obtain to 60 mesh；Half is that graininess wood chip and half are band in bamboo willow bits
Wood sawdust, the volume of graininess wood chip is 10mm3；The preparation method of microorganism nanometer selenium is in the present embodiment：By selenite
Be prepared into the solution of 0.005mol/L~0.05mol/L, by bacillus licheniformis according to mixing account for selenite solution 0.1%~
0.3% ratio is added into selenite solution；And 24~48h is kept at a temperature of 28~35 DEG C, low temperature is utilized after crystallization
It is dried to obtain；It is well mixed with the spirit of vinegar that mass fraction is 0.5% with wood sawdust, regulation PH is 6；In 32~38 DEG C of conditions
Under, add cellulase reaction 8h to obtain, cellulase is circumscribed β~dextranase, inscribe β~dextranase and β~grape
One or more in glycosidase；It is 12~15% that napier grass is dried to moisture content for fresh napier grass；Rub broken after chopping up, with quality point
Number is 6 for 0.5% spirit of vinegar regulation PH.
Preparation method is：All raw materials are sufficiently mixed uniformly；The pyrolkigneous liquid that mass fraction is 0.1% is added, water is kept
Point content is between 50~55%；After well mixed, sealing and fermenting 15 days makes its abundant water swelling and softening, that is, obtain black
Fungus cultivation matrix；
(2) making of edible black fungus bag：During the cultivation of auricularia auricula matrix that step (1) is obtained entered into polypropylene plastics bacterium bag；
Cultivation matrix accounts for the 70%~80% of the bacterium bag inner space；Stomata is pierced in the side of bacterium bag and bottom surface；
(3) edible black fungus bag sterilizing：The bacterium bag of the loading matrix that step (2) is obtained is 120 DEG C, high steam in temperature
Sterilizing 3h；After end subject to sterilization, normal temperature is returned to, take out bacterium bag；
(4) it is inoculated with：Under 23~26 DEG C of gnotobasis, access is routinely cultivated, bacterium ball is uniform in size, energetic
Agaric liquid spawn, access amount is 15ml/ bags；And one layer of gauze soaked with limewash is covered on bacterium bag surface, after inoculation
In sending out bacterium in room temperature, shading, dry culturing room；
(5) cultivation：At 15 DEG C~24 DEG C, air humidity is maintained at 50%~60% to culturing room's temperature control, every 3~5
It carries out a miscellaneous bacteria and checks and send out the inspection of bacterium situation, timely supplement inoculation and superseded miscellaneous bacteria；When the grain of rice occurs in bacterium bag surface
During shape ear base, ventilation 2~3 times is kept daily；Continue to draw a hole in bacterium bag surrounding, specially the whole body of bacterium bag four draw " V " or
"×" type mouthful, rule a length of 2.5~3cm, and depth is 0.3~0.5cm, and angle is 45~60 degree ,/bag of quantity about 5~10；Training
Bacterium bag is moved into agaric growing field after supporting 5~6 days carries out ear management；
(6) ear management：Produce agaric temperature control widens day and night temperature control at 9 DEG C~12 DEG C at 15 DEG C~25 DEG C, empty
Gas relative humidity is maintained at 80%~95%；In good time ventilation.
Embodiment 2
A kind of method of utilization tea tree branch cultivating black fungus, comprises the following steps：
(1) making of cultivation matrix：Raw material is constituted by the parts by weight of following raw material：45 parts of tea tree branch, bamboo willow bits
35 parts, 15 parts of napier grass, 2 parts of pulverized limestone, 2 parts of gypsum, 8 parts of dregs of beans, 2 parts of oyster shell whiting, 5 parts of medical stone, 5 parts of diatomite, phosphoric acid hydrogen
3 parts of calcium, 6 parts of glucose, 8 parts of sucrose, 2 parts of calcium superphosphate, 0.4 part of molybdenum trisulfate, 0.3 part of phosphoric acid hydrogen ammonia, 0.5 part of copper sulphate, sulphur
Sour ferrous iron 0.6 part, 0.05 part of microorganism nanometer selenium；Tea tree branch from through drying to solid water content of matter be 12~13%,
It is raw material without the tea tree branch that goes mouldy, it is size-reduced to obtain to 70 mesh；Half is that graininess wood chip and half are band in bamboo willow bits
Wood sawdust, the volume of graininess wood chip is 15mm3；The preparation method of microorganism nanometer selenium is with embodiment 1 in the present embodiment；Band saw
Wood chip is well mixed with the spirit of vinegar that mass fraction is 0.8%, and regulation PH is 5；Under the conditions of 32~38 DEG C, cellulose is added
Enzyme reaction 9h is obtained, and cellulase is the one kind in circumscribed β~dextranase, inscribe β~dextranase and β~glucuroide
Or it is several；It is 12~15% that napier grass is dried to moisture content for fresh napier grass；Rub broken after chopping up, it is 5 to adjust PH with spirit of vinegar；
Preparation method is：All raw materials are sufficiently mixed uniformly；The pyrolkigneous liquid that mass fraction is 0.2% is added, water is kept
Point content is between 50~55%；After well mixed, sealing and fermenting 10 days makes its abundant water swelling and softening, that is, obtain black
Fungus cultivation matrix；
(2) making of edible black fungus bag：During the cultivation of auricularia auricula matrix that step (1) is obtained entered into polypropylene plastics bacterium bag；
Cultivation matrix accounts for the 70%~80% of the bacterium bag inner space；Stomata is pierced in the side of bacterium bag and bottom surface；
(3) edible black fungus bag sterilizing：The bacterium bag of the loading matrix that step (2) is obtained is 122 DEG C, high steam in temperature
Sterilizing 3h；After end subject to sterilization, normal temperature is returned to, take out bacterium bag；
(4) it is inoculated with：Under 23~26 DEG C of gnotobasis, access is routinely cultivated, bacterium ball is uniform in size, energetic
Agaric liquid spawn, access amount is 20ml/ bags；And one layer of gauze soaked with limewash is covered on bacterium bag surface, after inoculation
In sending out bacterium in room temperature, shading, dry culturing room；
(5) cultivation：At 15 DEG C~24 DEG C, air humidity is maintained at 50%~60% to culturing room's temperature control, every 3~5
It carries out a miscellaneous bacteria and checks and send out the inspection of bacterium situation, timely supplement inoculation and superseded miscellaneous bacteria；When the grain of rice occurs in bacterium bag surface
During shape ear base, ventilation 2~3 times is kept daily；Continue to draw hole in bacterium bag surrounding, bacterium bag is moved into agaric growing field by culture after 5~6 days
Carry out ear management；
(6) ear management：Produce agaric temperature control widens day and night temperature control at 9 DEG C~12 DEG C at 15 DEG C~25 DEG C, empty
Gas relative humidity is maintained at 80%~95%；In good time ventilation.
Embodiment 3
A kind of method of utilization tea tree branch cultivating black fungus, comprises the following steps：
(1) making of cultivation matrix：Raw material is constituted by the parts by weight of following raw material：50 parts of tea tree branch, bamboo willow bits
40 parts, 20 parts of napier grass, 5 parts of pulverized limestone, 3 parts of gypsum, 10 parts of dregs of beans, 3 parts of oyster shell whiting, 6 parts of medical stone, 6 parts of diatomite, phosphoric acid hydrogen
4 parts of calcium, 8 parts of glucose, 10 parts of sucrose, 3 parts of calcium superphosphate, 0.6 part of molybdenum trisulfate, 0.6 part of phosphoric acid hydrogen ammonia, 0.8 part of copper sulphate, sulphur
Sour ferrous iron 0.8 part, 0.08 part of microorganism nanometer selenium；Tea tree branch from through drying to solid water content of matter be 12~13%,
It is raw material without the tea tree branch that goes mouldy, it is size-reduced to obtain to 80 mesh；Half is that graininess wood chip and half are band in bamboo willow bits
Wood sawdust；The volume of graininess wood chip is 18mm3；The preparation method of microorganism nanometer selenium is with embodiment 1 in the present embodiment；Band saw
Wood chip is well mixed with the spirit of vinegar that mass fraction is 1%, and regulation PH is 4；Under the conditions of 32~38 DEG C, cellulase is added
Reaction 8h is obtained, cellulase be one kind in circumscribed β~dextranase, inscribe β~dextranase and β~glucuroide or
It is several；It is 12~15% that napier grass is dried to moisture content for fresh napier grass；Rub broken after chopping up, with the spirit of vinegar that mass fraction is 1%
Regulation PH is 4；
Preparation method is：All raw materials are sufficiently mixed uniformly；The pyrolkigneous liquid that mass fraction is 0.3% is added, water is kept
Point content is between 50~55%；After well mixed, sealing and fermenting 8 days makes its abundant water swelling and softening, that is, obtain black wood
Ear cultivation matrix；
(2) making of edible black fungus bag：During the cultivation of auricularia auricula matrix that step (1) is obtained entered into polypropylene plastics bacterium bag；
Cultivation matrix accounts for the 70%~80% of the bacterium bag inner space；Stomata is pierced in the side of bacterium bag and bottom surface；
(3) edible black fungus bag sterilizing：The bacterium bag of the loading matrix that step (2) is obtained is 125 DEG C, high steam in temperature
Sterilizing 2h；After end subject to sterilization, normal temperature is returned to, take out bacterium bag；
(4) it is inoculated with：Under 23~26 DEG C of gnotobasis, access is routinely cultivated, bacterium ball is uniform in size, energetic
Agaric liquid spawn, access amount is 25ml/ bags；And one layer of gauze soaked with limewash is covered on bacterium bag surface, after inoculation
In sending out bacterium in room temperature, shading, dry culturing room；
(5) cultivation：At 15 DEG C~24 DEG C, air humidity is maintained at 50%~60% to culturing room's temperature control, every 3~5
It carries out a miscellaneous bacteria and checks and send out the inspection of bacterium situation, timely supplement inoculation and superseded miscellaneous bacteria；When the grain of rice occurs in bacterium bag surface
During shape ear base, ventilation 2~3 times is kept daily；Continue to draw hole in bacterium bag surrounding, bacterium bag is moved into agaric growing field by culture after 5~6 days
Carry out ear management；
(6) ear management：Produce agaric temperature control widens day and night temperature control at 9 DEG C~12 DEG C at 15 DEG C~25 DEG C, empty
Gas relative humidity is maintained at 80%~95%；In good time ventilation.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (9)
1. a kind of method of utilization tea tree branch cultivating black fungus, it is characterised in that：Comprise the following steps：
(1) making of cultivation matrix：Raw material is constituted by the parts by weight of following raw material：40~50 parts of tea tree branch, bamboo willow bits
30~40 parts, 10~20 parts of napier grass, 1~5 part of pulverized limestone, 1~3 part of gypsum, 5~10 parts of dregs of beans, 1~3 part of oyster shell whiting, medical stone
3~6 parts, 4~6 parts of diatomite, 2~4 parts of calcium monohydrogen phosphate, 5~8 parts of glucose, 6~10 parts of sucrose, 1~3 part of calcium superphosphate, sulphur
0.2~0.6 part of sour molybdenum, 0.1~0.6 part of phosphoric acid hydrogen ammonia, 0.1~0.8 part of copper sulphate, 0.5~0.8 part of ferrous sulfate, microorganism
0.03~0.08 part of nanometer selenium；It is that 12~13%, nothing is gone mouldy that the tea tree branch is selected by drying to solid water content of matter
Tea tree branch is raw material, size-reduced to obtain to 60~80 mesh；Half is graininess wood chip and half is band in bamboo willow bits
Wood sawdust；
Preparation method is：All raw materials are sufficiently mixed uniformly；Pyrolkigneous liquid is added, holding moisture is between 50~55%；
After well mixed, sealing and fermenting 8~15 days makes its abundant water swelling and softening, that is, obtain cultivation of auricularia auricula matrix；
(2) making of edible black fungus bag：During the cultivation of auricularia auricula matrix that step (1) is obtained entered into polypropylene plastics bacterium bag；Cultivation
Matrix accounts for the 70%~80% of the bacterium bag inner space；Stomata is pierced in the side of bacterium bag and bottom surface；
(3) edible black fungus bag sterilizing：The bacterium bag of the loading matrix that step (2) is obtained is 120~125 DEG C, high pressure steaming in temperature
2~3h of vapour sterilizing；After end subject to sterilization, normal temperature is returned to, take out bacterium bag；
(4) it is inoculated with：Under 23~26 DEG C of gnotobasis, access is routinely cultivated, the agaric that bacterium ball is uniform in size, energetic
Liquid spawn, access amount is 15~25ml/ bags；And one layer of gauze soaked with limewash is covered on bacterium bag surface, after inoculation
In sending out bacterium in room temperature, shading, dry culturing room；
(5) cultivation：At 15 DEG C~24 DEG C, air humidity is maintained at 50%~60% to culturing room's temperature control, enters every 3~5 days
Miscellaneous bacteria of row checks and sends out the inspection of bacterium situation, timely supplement inoculation and superseded miscellaneous bacteria；When rice-shaped ear occurs in bacterium bag surface
During base, ventilation 2~3 times is kept daily；Continue to draw hole in bacterium bag surrounding, bacterium bag is moved into agaric growing field and carried out by culture after 5~6 days
Ear management；
(6) ear management：Produce agaric temperature control widens day and night temperature control in 9 DEG C~12 DEG C, air phase at 15 DEG C~25 DEG C
80%~95% is maintained to humidity；In good time ventilation.
2. according to claim 1 using the method for tea tree branch cultivating black fungus, it is characterised in that：Raw material presses following raw material
Parts by weight composition：45 parts of tea tree branch, bamboo willow consider to be worth doing 35 parts, 15 parts of napier grass, 2 parts of pulverized limestone, 2 parts of gypsum, 8 parts of dregs of beans,
2 parts of oyster shell whiting, 5 parts of medical stone, 5 parts of diatomite, 3 parts of calcium monohydrogen phosphate, 6 parts of glucose, 8 parts of sucrose, 2 parts of calcium superphosphate, sulfuric acid
0.4 part of molybdenum, 0.3 part of phosphoric acid hydrogen ammonia, 0.5 part of copper sulphate, 0.6 part of ferrous sulfate, 0.05 part of microorganism nanometer selenium.
3. the method for utilization tea tree branch cultivating black fungus according to claim 1 or claim 2, it is characterised in that：The microorganism
The preparation method of nanometer selenium is：Selenite is prepared into the solution of 0.005mol/L~0.05mol/L, by bacillus licheniformis
The ratio for accounting for selenite solution 0.1%~0.3% according to mixing is added into selenite solution；And in 28~35 DEG C of temperature
24~48h of lower holding, is obtained after crystallization using low temperature drying.
4. according to claim 1 using the method for tea tree branch cultivating black fungus, it is characterised in that：It is described to be with wood sawdust
Well mixed with spirit of vinegar, regulation PH is 4~6；Under the conditions of 32~38 DEG C, add cellulase to react 8~10h and obtain.
5. according to claim 1 using the method for tea tree branch cultivating black fungus, it is characterised in that：The graininess wood chip
Volume be 10~18mm3。
6. according to claim 1 using the method for tea tree branch cultivating black fungus, it is characterised in that：The napier grass is fresh
It is 12~15% that napier grass is dried to moisture content；Rub broken after chopping up, it is 4~6 to adjust PH with spirit of vinegar.
7. according to claim 1 using the method for tea tree branch cultivating black fungus, it is characterised in that：The cellulase is
One or more in circumscribed β~dextranase, inscribe β~dextranase and β~glucuroide.
8. the preparation method of the method for tea tree branch cultivating black fungus is utilized according to claim 4 or 6, it is characterised in that；
In the step (1), the mass fraction of the spirit of vinegar is 0.5%~1%.
9. the preparation method of the method for tea tree branch cultivating black fungus is utilized according to claim 1, it is characterised in that：It is described
In step (1), the mass fraction of the pyrolkigneous liquid is 0.1%~0.3%.
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