CN106260589A - A kind of method that recycling wheat bran prepares albumen feedstuff - Google Patents
A kind of method that recycling wheat bran prepares albumen feedstuff Download PDFInfo
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- CN106260589A CN106260589A CN201610690865.4A CN201610690865A CN106260589A CN 106260589 A CN106260589 A CN 106260589A CN 201610690865 A CN201610690865 A CN 201610690865A CN 106260589 A CN106260589 A CN 106260589A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a kind of method that recycling wheat bran prepares albumen feedstuff, utilize biological enzymolysis and microbial fermentation technology, prepared by " two step enzymolysis+multi-cultur es four step solid fermentation " technique a kind of be of high nutritive value, the Wheat Brag Protein feedstuff of good palatability, the Forage Nutritive Value realizing wheat bran is high-valued, product is golden yellow, having light lactic acid taste, good palatability, in fermentation wheat bran, crude protein content is risen to 21.67% after fermentation by 14.2% before fermenting；And rich in nutritional labelings such as substantial amounts of useful viable bacteria, multivitamin, enzymes, there is enhancing development, raising efficiency of feed utilization, enhancing immunity, improvement intestinal microecology, prevent the advantages such as disease.The method uses solid fermentation, and production technology is simple, and energy consumption is low, non-environmental-pollution, investment is little, be prone to large-scale production.
Description
Technical field
The present invention relates to a kind of method that recycling wheat bran prepares albumen feedstuff, belong to field of feed processing.
Background technology
Protein raw material is the basis of feed industrial development, and the in short supply and price of China's protein raw material is surging to be had become as
The bottleneck of restriction animal husbandry development.Raising now with people's living standard and the attention to quality of life, to meat, egg, milk,
The demand of aquatic products is increasing, and grain and Feed Manufacturing face a severe test, and feed protein resource will be day by day deficient, therefore carry
The high grain transformation efficiency to livestock products and the utilization rate of feedstuff, expand feed resource, and exploitation unconventional water resources has been compeled at eyebrow
Eyelash.And the Critical policies alleviated the problems is aiming at existing protein sources, improving its utilization rate, that excavates feedstuff as much as possible can profit
By nutritional labeling, fundamentally alleviate the situation that feedstuff is in short supply.
Agricultural solid residue with corn straw, wheat bran, distiller grains etc. as representative and processing of farm products by-product, resource is rich
Richness, cheap, it is traditional feedstuff, but, this kind of feedstuff crude fiber content is higher, and nutritive value is on the low side, no
Be suitable to the development of modern efficient animal husbandry.Utilize the microbial metabolism effect in solid fermentation process, can effectively reduce raw material
Middle crude fibre, improves the content of the nutrition compositions such as protein；On the other hand, owing to microorganism growth process producing substantial amounts of dimension
The bioactive substances such as raw element, aminoacid, therefore the nutritive value of fermented feed significantly improves；Can also be by feed fermentation
During add probiotic bacteria, improve the Tiny ecosystem of animal intestines and stomach, improve the immunocompetence of body；In the fermentation of common feed solids
Yeast can be added, to increase tropina content, fermented feed can be made to have strong sweet-smelling simultaneously, improve the suitable of feedstuff
Mouth property, increases animal feed intake, improves production capacity.
In wheat bran in addition to containing more glucide, also have the most more rich in protein, its content 14% with
On, and the nutritive value of Wheat Brag Protein and biological value be superior to wheat protein.Contain this is because it has higher lysine
Amount and the several amino acids of needed by human.China's Testa Tritici aboundresources, whole world year processing wheat bran amount 2～300,000,000 tons, China
Year processing wheat bran amount more than 20,000,000 tons, but the most domestic substantially wheat bran is directly applied to feed industry, it is rarely used in deep
Processing and recycling, therefore economic worth is the highest.The high-valued feed industry that is applied to of the most relevant Testa Tritici albumen needs advanced skill badly
The development and application of art.
Summary of the invention
Pin the problems referred to above of the present invention, utilize microbial fermentation technology, it is provided that one utilizes enzymolysis and fermentation technology coordinate system
Standby be of high nutritive value, the Wheat Brag Protein feedstuff of good palatability, thus improve Forage Nutritive Value and the Forage quality of wheat bran.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of method that recycling wheat bran prepares albumen feedstuff, it is characterised in that specifically include following step
Rapid:
(1) pretreatment: choose the wheat bran without going mouldy, pulverized 40 mesh sieves, adjusted wheat bran water content 12～18%；
(2) steaming and decocting: using steam by wheat bran steaming and decocting, digestion time controls 40～60min；
(3) one step enzymolysis；Wheat bran liquid after cooling adds mass ratio 3～5% saccharifying enzyme, 2～3% amylase, temperature control 30～
Enzymolysis 20～40min under the conditions of 40 DEG C；
(4) preparation solid medium: the wheat bran after enzymolysis adds wheat bran mass ratio 5～8% molasses, 2～3% Semen Maydis pulp, 2～
3% ammonium nitrate, 1～1.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjust moisture content in medium 30～40%；
(5) aspergillosis activation: take fresh wheat bran, removes fine powder, in wheat bran with 60 mesh sieve sieves: water=1:1.0～1.3 ratios add
Water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, being distributed in 2000mL triangular flask, every 2000mL triangle is bottled wet
Wheat bran about 100g, sterilizing 40～60min under 0.1MPa gauge pressure, it is cooled to 35 DEG C, each triangular flask accesses 1-2 ring the most alive
The inclined-plane trichoderma reesei changed, aspergillus niger sp-1 spore, culture medium product temperature control is at 35 ± 1 DEG C. every 12h shaking flask once, and spore
After son grows, spore suspension is prepared in stopping；
(6) one-step fermentation: trichoderma reesei, aspergillus niger are inoculated in mix in the ratio of 3～5%, 2～3%, mix thoroughly 35～
40 DEG C of cultivations, aerlbic culture 12～18h, periodic agitation；
(7) two step enzymolysis: add 3～5% non-starch polysaccharide enzyme, 2～3% xylanase, 2～3% saccharifying in the wheat bran after fermentation
Enzyme, enzymolysis 20～40min under the conditions of temperature control 30～40 DEG C；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) two-step fermentation: Candida utilis, saccharomyces cerevisiae are seeded to culture medium by the inoculative proportion of 2～3%, 3～5%, 28
～35 DEG C of aerlbic cultures 18～24h, periodic agitation；
(10) bacillus subtilis, Bacillus licheniformis activation: use LB culture medium to cultivate 24h under the conditions of 28～32 DEG C, system
Obtain liquid seeds；
(11) three steps fermentations: bacillus subtilis and Bacillus licheniformis are connect in the ratio of wheat bran mass ratio 2～3%, 1～2%
Plant to culture medium, 28～35 DEG C of aerlbic cultures 16～24h, periodic agitation；
(12) four step fermentations: solid lactic acid mycopowder is seeded to culture medium by the inoculative proportion of 2～4%, 26～35 DEG C of Anaerobic culturel
16～24h；
(13) it is dried: after fermentation ends, low-temperature aeration is dried；
(14) pulverize: dried fermentation wheat bran is pulverized and gets product.
In above-mentioned steps (3), solid medium is without sterilizing.
The present invention provides a kind of method that recycling wheat bran prepares albumen feedstuff, is greatly improved the feeding of wheat bran
Nutritive value: product is golden yellow, has light lactic acid taste, and good palatability, before in fermentation wheat bran, crude protein content is by fermenting
14.2% rises to after fermentation 21.67%；Before and after mensuration fermentation, in wheat bran, free aminoacid content reaches the highest 24.76%；Survey
Surely fermentation bran protein composition, little peptide content is risen to 19.87% after fermentation by 3.56% before fermenting；Breast in fermentation wheat bran
Acid content reaches 8.7g/kg；Additionally, containing nutritional labelings such as substantial amounts of useful viable bacteria, multivitamin, enzymes in fermentation wheat bran.
Result of the test shows to change fermentation and enzymolysis process can significantly reduce effect (P < 0.01), such as work of two-step fermentation and three steps being fermented
When skill merges, protein content is only 19.3%.
The present invention provides a kind of method that recycling wheat bran prepares albumen feedstuff, according to basal diet formula (wheat bran
11%, Semen Maydis 65%, bean cake 20%, premix material 4%, matched group wheat bran uses common wheat bran) it being applied to growing and fattening pigs production, feed intake increases
Adding 9.8%, feed-weight ratio have dropped 12.5%, and Average weight increasing a day improves 13.1%, and compares without using antibiotic with matched group, takes
Obtained unforeseeable technique effect.
The present invention provides a kind of method that recycling wheat bran prepares albumen feedstuff, has an advantage in that: (1) passes through steaming and decocting
Processing with complex enzyme hydrolysis, cellulose, the xylan etc. that are difficult to microorganism utilize are degraded to reducing sugar, and content of reducing sugar rises to
The growth and breeding of microorganism provides the carbon source of abundance；(2) aspergillus niger and trichoderma reesei are to be currently known degradation of fibers effect
Significantly bacterial strain, is degraded the antinutritional factor such as cellulose, lignin in wheat bran by two-step fermentation technique, and is led to by mycete
The structural carbohydrate of wheat bran is degraded to the monosaccharide materials such as the available monosaccharide and disaccharide of yeast by the ability of words starch；
(3) degraded further the antinutritional factor in wheat bran by two step enzymolysis process, improve the nutrition digestibility of wheat bran, and be follow-up
Probiotics fermention sufficient carbon source is provided；(4) many bacterium mixed fungus fermentation, combination strain fermentation is used to add the many in fermentation
Gene function, by the combination of different metabolic ability, completes the complicated metabolism that single strain has been difficult to, passes through microorganism
Between reciprocal and laterality improve production efficiency, the existence of saccharomyces cerevisiae and Candida utilis promotes the degraded of mycete and makees
With, the amount reproduction of yeast adds the tropina in product, bacillus subtilis and Bacillus licheniformis and improves product
Middle probiotic bacteria content, improves effect of product；Lactic acid bacteria produces lactic acid etc. then can improve the palatability of fermentation wheat bran.Collaborative
Ferment form serves important positive role to the effectively conversion of various raw materials, the quality raising of albumen feedstuff；(5) multistep is used
Fermentation, fermentation strain requires difference, different reaction controlling conditions to temperature, pH, logical oxygen etc., improves the growth speed of thalline
Rate, shortens fermentation time, improves ferment effect；(6) without sterilizing in sweat, it is to avoid because of high temperature in sterilization process
The aminoacid caused and carbohydrate reaction thus cause the problem that protein digestibility declines；(7) in product rich in probiotic bacteria,
The nutritional labeling such as vitamin, enzyme and aminoacid, small peptide, high quality protein, have enhancing development, improve efficiency of feed utilization,
Enhancing immunity, improve intestinal microecology, prevent disease, reduce the advantage such as environmental pollution；(8) use solid fermentation, produce work
Skill is simple, and energy consumption is low, non-environmental-pollution, investment is little, be prone to large-scale production.
Detailed description of the invention
Embodiment 1:
A kind of method that recycling wheat bran prepares albumen feedstuff, concrete preparation process is as follows:
(1) pretreatment: choose the wheat bran without going mouldy, pulverized 40 mesh sieves, adjusted wheat bran water content 12～18%；
(2) steaming and decocting: using steam by wheat bran steaming and decocting, digestion time controls 40～60min；
(3) one step enzymolysis；Wheat bran liquid after cooling adds mass ratio 5% saccharifying enzyme, 3% amylase, temperature control 30～40 DEG C of bars
Enzymolysis 20min under part；
(4) preparation solid medium: in the wheat bran after enzymolysis add wheat bran mass ratio 8% molasses, 3% Semen Maydis pulp, 3% ammonium nitrate,
1.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, adjust moisture content in medium 30～40%；
(5) aspergillosis activation: take fresh wheat bran, removes fine powder, in wheat bran with 60 mesh sieve sieves: water=1:1.0～1.3 ratios add
Water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, being distributed in 2000mL triangular flask, every 2000mL triangle is bottled wet
Wheat bran about 100g, sterilizing 40～60min under 0.1MPa gauge pressure, it is cooled to 35 DEG C, each triangular flask accesses 1-2 ring the most alive
The inclined-plane trichoderma reesei changed, aspergillus niger sp-1 spore, culture medium product temperature control is at 35 ± 1 DEG C. every 12h shaking flask once, and spore
After son grows, spore suspension is prepared in stopping；
(6) one-step fermentation: trichoderma reesei, aspergillus niger are inoculated in mix in the ratio of 5%, 3%, mixes 35～40 DEG C of trainings thoroughly
Support, aerlbic culture 12h, periodic agitation；
(7) two step enzymolysis: add 5% non-starch polysaccharide enzyme, 3% xylanase, 3% saccharifying enzyme, temperature control 30 in the wheat bran after fermentation
～enzymolysis 20min under the conditions of 40 DEG C；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) two-step fermentation: Candida utilis, saccharomyces cerevisiae are seeded to culture medium by the inoculative proportion of 3%, 5%, 28～35 DEG C
Aerlbic culture 18h, periodic agitation；
(10) bacillus subtilis, Bacillus licheniformis activation: use LB culture medium to cultivate 24h under the conditions of 28～32 DEG C, system
Obtain liquid seeds；
(11) three step fermentations: bacillus subtilis and Bacillus licheniformis are seeded to training in the ratio of wheat bran mass ratio 3%, 2%
Support base, 28～35 DEG C of aerlbic culture 16h, periodic agitation；
(12) four step fermentations: solid lactic acid mycopowder is seeded to culture medium by the inoculative proportion of 4%, 26～35 DEG C of Anaerobic culturel
16h；
(13) it is dried: after fermentation ends, low-temperature aeration is dried；
(14) pulverize: dried fermentation wheat bran is pulverized and gets product.
Embodiment 2:
A kind of method that recycling wheat bran prepares albumen feedstuff, concrete preparation process is as follows:
(1) pretreatment: choose the wheat bran without going mouldy, pulverized 40 mesh sieves, adjusted wheat bran water content 12～18%；
(2) steaming and decocting: using steam by wheat bran steaming and decocting, digestion time controls 40～60min；
(3) one step enzymolysis；Wheat bran liquid after cooling adds mass ratio 3% saccharifying enzyme, 2% amylase, temperature control 30～40 DEG C of bars
Enzymolysis 40min under part；
(4) preparation solid medium: in the wheat bran after enzymolysis add wheat bran mass ratio 5% molasses, 2% Semen Maydis pulp, 2% ammonium nitrate,
1% ammonium sulfate, 0.1% potassium dihydrogen phosphate, adjust moisture content in medium 30～40%；
(5) aspergillosis activation: take fresh wheat bran, removes fine powder, in wheat bran with 60 mesh sieve sieves: water=1:1.0～1.3 ratios add
Water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, being distributed in 2000mL triangular flask, every 2000mL triangle is bottled wet
Wheat bran about 100g, sterilizing 40～60min under 0.1MPa gauge pressure, it is cooled to 35 DEG C, each triangular flask accesses 1-2 ring the most alive
The inclined-plane trichoderma reesei changed, aspergillus niger sp-1 spore, culture medium product temperature control is at 35 ± 1 DEG C. every 12h shaking flask once, and spore
After son grows, spore suspension is prepared in stopping；
(6) one-step fermentation: trichoderma reesei, aspergillus niger are inoculated in mix in the ratio of 3%, 2%, mixes 35～40 DEG C of trainings thoroughly
Support, aerlbic culture 18h, periodic agitation；
(7) two step enzymolysis: add 3% non-starch polysaccharide enzyme, 2% xylanase, 2% saccharifying enzyme, temperature control 30 in the wheat bran after fermentation
～enzymolysis 40min under the conditions of 40 DEG C；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) two-step fermentation: Candida utilis, saccharomyces cerevisiae are seeded to culture medium by the inoculative proportion of 2%, 3%, 28～35 DEG C
Aerlbic culture 24h, periodic agitation；
(10) bacillus subtilis, Bacillus licheniformis activation: use LB culture medium to cultivate 24h under the conditions of 28～32 DEG C, system
Obtain liquid seeds；
(11) three step fermentations: bacillus subtilis and Bacillus licheniformis are seeded to training in the ratio of wheat bran mass ratio 2%, 1%
Support base, 28～35 DEG C of aerlbic culture 24h, periodic agitation；
(12) four step fermentations: solid lactic acid mycopowder is seeded to culture medium by the inoculative proportion of 2%, 26～35 DEG C of Anaerobic culturel
24h；
(13) it is dried: after fermentation ends, low-temperature aeration is dried；
(14) pulverize: dried fermentation wheat bran is pulverized and gets product.
Embodiment 3:
A kind of method that recycling wheat bran prepares albumen feedstuff, concrete preparation process is as follows:
(1) pretreatment: choose the wheat bran without going mouldy, pulverized 40 mesh sieves, adjusted wheat bran water content 12～18%；
(2) steaming and decocting: using steam by wheat bran steaming and decocting, digestion time controls 40～60min；
(3) one step enzymolysis；Wheat bran liquid after cooling adds mass ratio 4% saccharifying enzyme, 2.5% amylase, temperature control 30～40 DEG C
Under the conditions of enzymolysis 30min；
(4) preparation solid medium: in the wheat bran after enzymolysis add wheat bran mass ratio 6.5% molasses, 2.5% Semen Maydis pulp, 2.5%
Ammonium nitrate, 1.2% ammonium sulfate, 0.15 % potassium dihydrogen phosphate, adjust moisture content in medium 30～40%；
(5) aspergillosis activation: take fresh wheat bran, removes fine powder, in wheat bran with 60 mesh sieve sieves: water=1:1.0～1.3 ratios add
Water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, being distributed in 2000mL triangular flask, every 2000mL triangle is bottled wet
Wheat bran about 100g, sterilizing 40～60min under 0.1MPa gauge pressure, it is cooled to 35 DEG C, each triangular flask accesses 1-2 ring the most alive
The inclined-plane trichoderma reesei changed, aspergillus niger sp-1 spore, culture medium product temperature control is at 35 ± 1 DEG C. every 12h shaking flask once, and spore
After son grows, spore suspension is prepared in stopping；
(6) one-step fermentation: trichoderma reesei, aspergillus niger are inoculated in mix in the ratio of 4%, 2.5%, mix 35～40 DEG C thoroughly
Cultivate, aerlbic culture 15h, periodic agitation；
(7) two step enzymolysis: add 4% non-starch polysaccharide enzyme, 2.5% xylanase, 2.5% saccharifying enzyme in the wheat bran after fermentation, control
Enzymolysis 30min under the conditions of temperature 30～40 DEG C；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) two-step fermentation: Candida utilis, saccharomyces cerevisiae are seeded to culture medium by the inoculative proportion of 2.5%, 4%, 28～35
DEG C aerlbic culture 21h, periodic agitation；
(10) bacillus subtilis, Bacillus licheniformis activation: use LB culture medium to cultivate 24h under the conditions of 28～32 DEG C, system
Obtain liquid seeds；
(11) three step fermentations: bacillus subtilis and Bacillus licheniformis are inoculated in the ratio of wheat bran mass ratio 2.5%, 1.5%
To culture medium, 28～35 DEG C of aerlbic culture 20h, periodic agitation；
(12) four step fermentations: solid lactic acid mycopowder is seeded to culture medium by the inoculative proportion of 3%, 26～35 DEG C of Anaerobic culturel
20h；
(13) it is dried: after fermentation ends, low-temperature aeration is dried；
(14) pulverize: dried fermentation wheat bran is pulverized and gets product.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Execute example to be described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Execute the technical scheme described in example to modify, or wherein portion of techniques feature is carried out equivalent；And these are revised
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (1)
1. the method that a recycling wheat bran prepares albumen feedstuff, it is characterised in that specifically include following steps:
(1) pretreatment: choose the wheat bran without going mouldy, pulverized 40 mesh sieves, adjusted wheat bran water content 12～18%；
(2) steaming and decocting: using steam by wheat bran steaming and decocting, digestion time controls 40～60min；
(3) one step enzymolysis；Wheat bran liquid after cooling adds mass ratio 3～5% saccharifying enzyme, 2～3% amylase, temperature control 30～
Enzymolysis 20～40min under the conditions of 40 DEG C；
(4) preparation solid medium: the wheat bran after enzymolysis adds wheat bran mass ratio 5～8% molasses, 2～3% Semen Maydis pulp, 2～
3% ammonium nitrate, 1～1.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjust moisture content in medium 30～40%；
(5) aspergillosis activation: take fresh wheat bran, removes fine powder, in wheat bran with 60 mesh sieve sieves: water=1:1.0～1.3 ratios add
Water, mix thoroughly to without dry powder again without clustering phenomena, after mixing thoroughly, be distributed in 2000mL triangular flask, every 2000mL triangle is bottled wet
Wheat bran about 100g, sterilizing 40～60min under 0.1MPa gauge pressure, it is cooled to 35 DEG C, each triangular flask accesses 1-2 ring the most alive
The inclined-plane trichoderma reesei changed, aspergillus niger sp-1 spore, culture medium product temperature control is at 35 ± 1 DEG C. every 12h shaking flask once, and spore
After son grows, spore suspension is prepared in stopping；
(6) one-step fermentation: trichoderma reesei, aspergillus niger are inoculated in mix in the ratio of 3～5%, 2～3%, mix thoroughly 35～
40 DEG C of cultivations, aerlbic culture 12～18h, periodic agitation；
(7) two step enzymolysis: add 3～5% non-starch polysaccharide enzyme, 2～3% xylanase, 2～3% saccharifying in the wheat bran after fermentation
Enzyme, enzymolysis 20～40min under the conditions of temperature control 30～40 DEG C；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) two-step fermentation: Candida utilis, saccharomyces cerevisiae are seeded to culture medium by the inoculative proportion of 2～3%, 3～5%, 28
～35 DEG C of aerlbic cultures 18～24h, periodic agitation；
(10) bacillus subtilis, Bacillus licheniformis activation: use LB culture medium to cultivate 24h under the conditions of 28～32 DEG C, system
Obtain liquid seeds；
(11) three steps fermentations: bacillus subtilis and Bacillus licheniformis are connect in the ratio of wheat bran mass ratio 2～3%, 1～2%
Plant to culture medium, 28～35 DEG C of aerlbic cultures 16～24h, periodic agitation；
(12) four step fermentations: solid lactic acid mycopowder is seeded to culture medium by the inoculative proportion of 2～4%, 26～35 DEG C of Anaerobic culturel
16～24h；
(13) it is dried: after fermentation ends, low-temperature aeration is dried；
(14) pulverize: dried fermentation wheat bran is pulverized and gets product.
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| CN109744366A (en) * | 2019-03-21 | 2019-05-14 | 佛山奇诺福饲料科技有限公司 | A kind of feed addictive and its application method |
| CN110663820A (en) * | 2018-07-02 | 2020-01-10 | 辽宁波尔莱特农牧实业有限公司 | Method for preparing fermented bran through bacterium-enzyme synergistic fermentation |
| CN113575764A (en) * | 2021-06-24 | 2021-11-02 | 山东泰山生力源集团股份有限公司 | Saccharomyces cerevisiae culture and application thereof |
| CN114287515A (en) * | 2021-12-27 | 2022-04-08 | 郑州云益生物科技有限公司 | Method for preparing biological feed by using wheat processing by-products |
| CN114437950A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma GOY1 for improving aquatic animal body color and preparation method and application of culture of phaffia rhodozyma GOY1 |
| WO2024213599A1 (en) * | 2023-04-12 | 2024-10-17 | Dsm Ip Assets B.V. | Glucoamylase as binding agent for non-animal protein food products |
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| CN110663820A (en) * | 2018-07-02 | 2020-01-10 | 辽宁波尔莱特农牧实业有限公司 | Method for preparing fermented bran through bacterium-enzyme synergistic fermentation |
| CN109744366A (en) * | 2019-03-21 | 2019-05-14 | 佛山奇诺福饲料科技有限公司 | A kind of feed addictive and its application method |
| CN113575764A (en) * | 2021-06-24 | 2021-11-02 | 山东泰山生力源集团股份有限公司 | Saccharomyces cerevisiae culture and application thereof |
| CN113575764B (en) * | 2021-06-24 | 2024-04-12 | 山东泰山生力源集团股份有限公司 | Saccharomyces cerevisiae culture and application thereof |
| CN114437950A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma GOY1 for improving aquatic animal body color and preparation method and application of culture of phaffia rhodozyma GOY1 |
| CN114437950B (en) * | 2021-12-24 | 2023-11-24 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma GOY1 for improving body color of aquatic animals and preparation method and application of culture of Phaffia rhodozyma GOY |
| CN114287515A (en) * | 2021-12-27 | 2022-04-08 | 郑州云益生物科技有限公司 | Method for preparing biological feed by using wheat processing by-products |
| WO2024213599A1 (en) * | 2023-04-12 | 2024-10-17 | Dsm Ip Assets B.V. | Glucoamylase as binding agent for non-animal protein food products |
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