CN106244453B - A kind of cell culture device for cultivating, measuring and analyzing - Google Patents
A kind of cell culture device for cultivating, measuring and analyzing Download PDFInfo
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- CN106244453B CN106244453B CN201610708334.3A CN201610708334A CN106244453B CN 106244453 B CN106244453 B CN 106244453B CN 201610708334 A CN201610708334 A CN 201610708334A CN 106244453 B CN106244453 B CN 106244453B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention discloses a kind of cell culture devices for cultivating, measuring and analyzing, including pedestal and several culture vessels, each culture vessel stacks gradually, culture vessel includes ware body, column and liquid injection pipe, and column upper/lower terminal is open, and a rotation pipe passes through all columns, rotation pipe can be relatively rotated with column, and it is in sealing contact between rotation pipe and column, rotation pipe lateral wall is equipped with sump pit, in a culture vessel：Column is equipped with the equal liquid hole being connected to inside ware body, cell filtering net and equal liquid check valve are equipped in equal liquid hole, equal liquid check valve is between cell filtering net and rotation pipe, and equal liquid check valve can be to be connected to inside liquid injection pipe lower end and ware body by external to ware in ware body by direction.The beneficial effects of the invention are as follows：Suitable for cell culture and measurement, comparison, rationally and stable structure, operating process is more convenient, and the adjusting consistency during testing is good, accuracy is high for space layout.
Description
Technical field
The invention belongs to technical field of cell culture more particularly to a kind of cell cultures for cultivating, measuring and analyzing
Device.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, for maintaining the nutrient matrix of cell growth to be known as culture medium.Culture medium can be divided by its physical state
Fluid nutrient medium and solid medium.Cell culture technology is more for testing at present other than being applied to production（It is such as right
Group sample parameter is measured, and is compared, analyzes and is studied）, and test in it is more commonly used to be fluid nutrient medium.This
Outside, cell culture needs to use cell culture apparatus, common are culture dish, incubator etc., the cell culture under general environment
Culture dish can be directly used, some particular surroundings are such as needed（It is such as powered, heats）, then special incubator can be used.
At present when carrying out cell culture and Comparability test in the process, carried for culture medium although common culture apparatus can play
For the function of bearing space, but in space layout reasonability, integrated operation convenience, regulating effect consistency etc., still have
It is short of.
Invention content
Being suitable for cell culture, measurement and analysis it is an object of the invention to overcome the defect of prior art, and provide a kind of
It compares, rationally and stable structure, operating process is more convenient, and the adjusting consistency during testing is good, accuracy for space layout
High breeding apparatus.
To achieve the goals above, the present invention uses following technical scheme：
A kind of cell culture device for cultivating, measuring and analyzing includes the incubator of pedestal and several upper end openings
Ware, each culture vessel stack gradually from the bottom to top, and a culture vessel of bottom contacts pedestal, and the pedestal, which is equipped with, to be fixed
Upright bar, culture vessel lateral wall are equipped with the retaining collar being adapted to fixed upright bar, and retaining collar is set in fixed upright bar, is trained
The liquid injection pipe that foster vessel include ware body, the column in ware body on bottom is arranged and is arranged on ware body sidewall and is in tilted layout,
Column upper/lower terminal is open, and a rotation pipe arranged vertically passes through all columns, the rotation pipe opposite can to turn with column
Dynamic and in sealing contact between rotation pipe lateral wall and column madial wall, rotation pipe lateral wall, which is equipped with, runs through rotation pipe upper and lower two
The sump pit at end, in a culture vessel：Column is equipped with the equal liquid hole being connected to inside ware body, and cell is equipped in equal liquid hole
It is connected to inside filter screen, liquid injection pipe lower end and ware body.
Preferably, the liquid injection pipe upper end is equipped with the pipe lid with liquid injection pipe screw-thread fit, it is arranged with and is used in rotation pipe
The vessel cover that the upper end opening of one culture vessel of the top is sealed.
Preferably, the pedestal includes matrix and several support columns being arranged below matrix, matrix, which is equipped with, to be run through
Matrix bottom through-hole upper and lower surfaces of, rotation pipe lower end are stretched into the through-hole of bottom, and fluid cylinder is connect equipped with opening up below the through-hole of bottom,
Be equipped with bottom bracing plate connect at least one support column below matrix, bottom bracing plate equipped with the cylinder slot that connects fluid cylinder bottom and be adapted to,
Fluid cylinder bottom is connect to be in a slot.
Preferably, being equipped with core pipe and several horizontally disposed diaphragm plates in the rotation pipe, it is logical that bottom is stretched into core pipe lower end
In hole, support ring is arranged in core pipe, support ring is higher than core pipe bottom and support ring is in the through-hole of bottom, and branch is equipped in the through-hole of bottom
Seat is supportted, the annular groove for being equipped with seat slot at the top of support base and being adapted to rotation pipe lower end, support ring bottom contacts seat slot slot bottom, rotation pipe
Lower end is stretched into annular groove and rotation bottom of the tube contact annular groove slot bottom, rotation pipe are rotatably assorted with support base, and core pipe passes through support base
And core pipe is rotatably assorted with support base, diaphragm plate is set in core pipe, diaphragm plate cross section be in circular ring shape, rotation pipe, core pipe and
Each diaphragm plate is coaxial, and core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, diaphragm plate and rotation pipe
Between seal fix；
Diaphragm plate is equipped with even lumen pore, and core pipe is equipped with several hole sealing plates for the company's of sealing lumen pore, hole sealing plate and tabula
Plate corresponds, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts diaphragm plate
Top surface；
Quantitative chamber is formed in rotation pipe between adjacent two pieces of diaphragm plates, if rotation pipe is equipped with main pipe to fluid apertures, quantitative chamber
Fluid apertures one-to-one correspondence, quantitative chamber is given to be corresponded with culture vessel with pipe, in corresponding quantitative chamber and culture vessel：On column
Equipped with cylinder to fluid apertures, quantitative chamber is connected to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is in circle to fluid apertures to fluid apertures and cylinder
Cylindricality, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest point is given higher than cylinder
Fluid apertures lowest point, cylinder are higher than equal liquid hole highest point to fluid apertures lowest point.
Preferably, in corresponding quantitative chamber and culture vessel：Quantitative intracavitary is equipped with tune amount liquid capsule, is set in tune amount liquid capsule
It is useful for the liquid supply pipe of tune amount liquid capsule feed flow, liquid supply pipe one end is connected to tune amount liquid capsule, and liquid supply pipe is by being in quantitative intracavitary
Hard tube section and the outer segment being in outside quantitative chamber are constituted；
Pedestal is used for liquid supply pipe feed flow equipped with several for fluid cylinder, is included cylinder body for fluid cylinder, is slidingly sealed with cylinder body
The feed flow piston of cooperation and the piston rod for connecting and being in above feed flow piston with feed flow piston, the slidably direction of feed flow piston
For upper and lower directions, at one in fluid cylinder：Space in cylinder body below feed flow piston is sap cavity, in cylinder body above feed flow piston
Space is adjusting cavity；
Sap cavity is corresponded with liquid supply pipe, and the liquid supply pipe other end is connected to corresponding sap cavity, and liquid supply pipe passes through rotation pipe side
Wall and rotation pipe lower end seal between liquid supply pipe and rotation pipe side wall and fix.
Preferably, the pedestal is equipped with is equipped with horizontally disposed tune with the fixed adjustment seat of pedestal, adjustment seat lower section
Plate is saved, piston rod passes through adjustable plate, and equipped with upper holder block and lower drawing block, adjustment seat is equipped with vertical arrangement and across tune piston rod
The homophony pole of seat is saved, homophony pole is threadedly coupled with adjustment seat, and homophony pole is equipped with upper holder block and lower drawing block, upper holder block bottom
Face contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, is equipped with and the fixed master of homophony pole at the top of homophony pole
Adjusting knob.
Preferably, the homophony pole is equipped with the instruction disk being in above adjustment seat, indicate that disk and homophony pole are same
Axis, adjustment seat are equipped with the indicating arm of slot with a scale, and indicating arm is arranged vertically and indicating arm contact instruction disk side wall.
Preferably, be equipped with the sealing compression bar arranged vertically in the rotation pipe, sealing compression bar pass through all diaphragm plates and
It being threadedly coupled between sealing compression bar and all diaphragm plates, sealing compression bar is equipped with several pressure plates for compressing hole sealing plate,
Pressure plate is corresponded with hole sealing plate, and pressure plate is in the top of corresponding hole sealing plate.
The beneficial effects of the invention are as follows：Suitable for cell culture and measurement, comparison, space layout rationally and stable structure,
Operating process is more convenient, and the adjusting consistency during testing is good, accuracy is high.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the structural schematic diagram of orifice plate of the present invention；
Fig. 3 is the enlarged drawing at A in Fig. 1；
Fig. 4 is the enlarged drawing at B in Fig. 1；
Fig. 5 is the enlarged drawing at C in Fig. 1；
Fig. 6 is a sectional view at rotation pipe of the present invention.
In figure：Pedestal 1, fixed upright bar 11, to connect fluid cylinder 12, bottom bracing plate 13, support base 14, matrix 1a, support column 1b, bottom logical
Hole 1c, culture vessel 2, retaining collar 21, equal liquid hole 22, pipe lid 23, vessel cover 24, orifice plate 25, cylinder to fluid apertures 26, ware body 2a,
Column 2b, liquid injection pipe 2c, rotation pipe 3, sump pit 31, diaphragm plate 32, even lumen pore 321, pipe be to fluid apertures 33, tune amount liquid capsule 34, fixed
Measure chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, for fluid cylinder 5, cylinder body 5a, feed flow piston 5b, piston rod 5c, sap cavity 5d, adjust
Chamber 5e, adjustment seat 6, adjustable plate 61, upper holder block 611, lower drawing block 612, homophony pole 62, main regulation knob 621, instruction disk 622,
Indicating arm 63, sealing compression bar 7, pressure plate 71.
Specific implementation mode
The present invention will be further described with reference to the accompanying drawings and detailed description.
In embodiment as shown in Figures 1 to 6, a kind of cell culture device for cultivating, measuring and analyzing, including bottom
The culture vessel 2 of seat 1 and several upper end openings, each culture vessel stack gradually from the bottom to top, a culture vessel of bottom
Pedestal is contacted, the pedestal is equipped with fixed upright bar 11, and culture vessel lateral wall is equipped with the fixing sleeve being adapted to fixed upright bar
Ring 21, retaining collar are set in fixed upright bar, culture vessel includes ware body 2a, the column 2b that is arranged in ware body on bottom and
The liquid injection pipe 2c for being arranged on ware body sidewall and being in tilted layout, column upper/lower terminal are open, a rotation pipe arranged vertically
3 pass through all columns, the rotation pipe that can be relatively rotated with column, and sealing connects between rotation pipe lateral wall and column madial wall
It touches, rotation pipe lateral wall is equipped with the sump pit 31 through rotation pipe upper/lower terminal, in a culture vessel：It is set on column
There is the equal liquid hole 22 being connected to inside ware body, cell filtering net is equipped in equal liquid hole, is connected to inside liquid injection pipe lower end and ware body.
Retaining collar is set to be inserted in before experiment, when each culture vessel can from the bottom to top be stacked gradually to placement, and being placed solid
Determine upright bar, make outside column set to rotation pipe（Structure in rotation pipe and rotation pipe can be pre-set）, so that each culture
Vessel can be aligned, and the culture vessel of top just covers the culture vessel of lower section.Then liquid feeding is carried out to each liquid injection pipe（Add
Enter fluid nutrient medium or culture solution）, without deliberately controlling liquid volume added when liquid feeding, as long as liquid level lid crosses equal liquid hole.All add
After the completion of liquid, turn rotation pipe allows sump pit to be connected to each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid
Hole is flowed out, and remaining liquid measure reaches consistent in culture vessel, then can turn rotation pipe, allow sump pit to leave equal liquid hole（Make it
It is not connected to）, liquid feeding has so just been rapidly completed.
It should be pointed out that cell to be cultivated can just be placed in culture vessel before culture solution is added（It needs to wait cultivating
Cell of short duration can leave culture solution and non-inactivation or death）, at this time due to thering is the presence of cell filtering net, cell will not flow out
Culture vessel；It can also be added in culture solution, equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process）Cell to be cultivated is added later；Also the culture solution that band directly can be needed to be cultivated cell is directly injected into training
Support vessel（Experiment under the premise of this should not consider the influence of initial cell quantity, and the presence due to there is cell filtering net, cell
It will not flow out culture vessel）.It can specifically be selected according to test objective, demand.
The liquid injection pipe upper end is equipped with pipe lid 23 with liquid injection pipe screw-thread fit, is arranged in rotation pipe for by the top
A culture vessel the vessel cover 24 that seals of upper end opening.Pipe lid can block rotation pipe and extraneous connection, vessel cover
A culture vessel of the top and extraneous connection can be blocked, remaining culture vessel is then by a nearest training of itself top
Vessel are supported to block and extraneous connection.So as to ensure that each culture vessel separates during cell culture, experiment with the external world,
To allow the present invention to be applicable to the experiment under the premise of being isolated from the outside world.
The pedestal includes matrix 1a and several support column 1b being arranged below matrix, and matrix, which is equipped with, runs through matrix
Through-hole 1c in bottom upper and lower surfaces of, rotation pipe lower end are stretched into the through-hole of bottom, and fluid cylinder 12 is connect equipped with opening up below the through-hole of bottom,
Be equipped with the bottom bracing plate 13 connect at least one support column below matrix, bottom bracing plate equipped with the cylinder that connects fluid cylinder bottom and be adapted to
Slot connects fluid cylinder bottom and is in a slot.The liquid being discharged from sump pit can be recycled easily（It falls into and connects fluid cylinder）, can avoid wave
Take and avoids polluting the external world.
The column includes cylinder and orifice plate 25, and cylinder lateral wall is equipped with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made of mutually symmetrical with upper half plate with lower half plate, upper half plate and lower half
Plate is an integral molding structure, and equal liquid hole is located on upper half plate.In addition to equal liquid hole, orifice plate is structure symmetrical above and below, thus,
One piece of orifice plate can be achieved with the Liquid level of two kinds of different heights, that is, by orifice plate by upper half plate in upper and lower half plate in the form of lower
It is caught in side opening, then passes through equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）
Afterwards, liquid level is consistent with equal liquid hole lowest point in culture vessel（More than equal liquid hole is in the middle part of orifice plate at this time）；And by orifice plate
By upper half plate under, lower half plate side opening is caught in the form of upper, then liquid level and equal liquid hole lowest point in final culture vessel
Unanimously（Equal liquid hole is in following in the middle part of orifice plate at this time）.Furthermore, it is possible to configure more set orifice plates, there was only equal liquid hole between each set orifice plate
Height is different, so can be achieved with the quick control of various different initial liquid measures in culture vessel（Which liquid level is needed, just
A set of orifice plate corresponding with the liquid level is caught in advance and is used in the side opening of each culture vessel）.
It is equipped with core pipe 4 in the rotation pipe and several horizontally disposed diaphragm plates 32, core pipe lower end are stretched into the through-hole of bottom, core
Support ring 41 is arranged on pipe, support ring is higher than core pipe bottom and support ring is in the through-hole of bottom, and support base is equipped in the through-hole of bottom
14, the annular groove for being equipped with seat slot at the top of support base and be adapted to rotation pipe lower end, support ring bottom contact seat slot slot bottom, under rotation pipe
End is stretched into annular groove and rotation bottom of the tube contact annular groove slot bottom, rotation pipe are rotatably assorted with support base, core pipe across support base and
Core pipe is rotatably assorted with support base, and diaphragm plate is set in core pipe, and diaphragm plate cross section is in circular ring shape, rotation pipe, core pipe and each
Diaphragm plate is coaxial, and core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, diaphragm plate and rotation pipe it
Between seal fix；
Diaphragm plate be equipped with even lumen pore 321, core pipe be equipped with several hole sealing plates 42 for the company's of sealing lumen pore, hole sealing plate and
Diaphragm plate corresponds, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, and the contact of hole sealing plate bottom surface is horizontal
Partition board top surface；
Quantitative chamber 3a is formed in rotation pipe between adjacent two pieces of diaphragm plates, if rotation pipe is equipped with main pipe to fluid apertures 33, is determined
Measure chamber gives fluid apertures one-to-one correspondence, quantitative chamber to be corresponded with culture vessel with pipe, in corresponding quantitative chamber and culture vessel：It is perpendicular
Cylinder is equipped with cylinder to fluid apertures 26, and quantitative chamber is connected to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is to fluid apertures and cylinder to fluid apertures
It is cylinder, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest point is high
Fluid apertures lowest point, cylinder is given to be higher than equal liquid hole highest point to fluid apertures lowest point in cylinder.
When needs carry out mid-term liquid feeding simultaneously to each culture vessel（New culture solution, Ke Yiyu are added in incubation
Initial incubation liquid type, proportioning are identical, can also be different from initial incubation liquid type, proportioning）When, for each ware body, liquid
The time difference that body is added is smaller, and the interference of suffered external cause is also smaller when finally measuring（It is preferably capable of that culture is added simultaneously
Liquid）.But it unless more people is allowed to carry out liquid feeding to each culture vessel simultaneously, and to ensure height while property, can be only achieved more
Ideal effect, and in practical operation, the feasibility of this mode is relatively low.And in the present solution, first turn rotation pipe, allowing pipe
It is staggered between fluid apertures with corresponding cylinder to fluid apertures（It is not connected to）, then allow rotation pipe motionless, first turn core pipe（If rotation pipe with
Frictional force is sufficiently large between column, then can direct turn core pipe, at this time rotation pipe will not be driven；If rubbing between rotation pipe and column
Wiping power is limited, then rotation pipe can be pinched on the other hand, the other hand carrys out turn core pipe；The present invention is in design, preferential former feelings
Shape）, hole sealing plate is driven to leave even lumen pore（Allow the sealing plate not company's of sealing lumen pore）, liquid feeding, culture then can be carried out at the top of rotation pipe
Liquid can pass through each even lumen pore, and finally be full of all quantitative chambers, then proceed to make rotation pipe motionless, turn core pipe makes envelope
Orifice plate seals even lumen pore, separates between quantitative chamber each at this time, and each quantitative intracavitary liquid measure is consistent.Then can turn rotation pipe, lead to
Crossing diaphragm plate drives core pipe to rotate together（It is fixed between diaphragm plate and rotation pipe, although can phase between core pipe and diaphragm plate
To rotation, but necessarily there is the power compressed mutually due in sealing contact between core pipe and diaphragm plate, between core pipe and diaphragm plate
And frictional force needs to apply the external force for allowing them to relatively rotate so if relatively rotating.And only turn core pipe when, rubbing
Under the action of wiping power, diaphragm plate can be rotated together with core pipe, so relative position is constant between rotation pipe and core pipe）, allow
Pipe is connected to corresponding cylinder between fluid apertures to fluid apertures, at this time each quantify chamber can give corresponding ware body inside simultaneously, equivalent
Liquid feeding can thoroughly eliminate during intermediate liquid feeding the disturbing factors such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent.This
Outside, this programme is in addition to can be used for mid-term liquid feeding, it can also be used to liquid feeding when initially.And after having support base, it is arranged from sump pit
The liquid gone out can first reach at the top of support base, then flow down outward.
It is noted that when sump pit is connected to equal liquid hole, pipe is staggered with corresponding cylinder between fluid apertures to fluid apertures；Manage to
Fluid apertures with corresponding cylinder to being connected between fluid apertures when, sump pit is staggered with equal liquid hole.And pass through equal hydraulic control amount（I.e. mentioned by equal
Fluid apertures carries out drain so that the consistent process of liquid measure in each ware body）Afterwards, in the behaviour using quantitative chamber to progress liquid feeding in ware body
During work（When turn rotation pipe）, sump pit cannot be allowed to be connected to again with equal liquid hole, otherwise can lead to the leakage from sump pit
（Liquid is flowed out from equal liquid hole, sump pit again in ware body）, there are many kinds of specific constructive forms and mode of operation, in this measure two
Example,
Example 1：In the circumferential direction of rotation pipe, sump pit, equal liquid hole, pipe to fluid apertures, cylinder to fluid apertures along up time pointing needle successively
Arrangement.Equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）When operation, row
Liquid bath is clockwise to alignment equal liquid hole with rotation pipe（The process middle pipe is not connected to corresponding cylinder to fluid apertures to fluid apertures）,
After the completion of hydraulic control amount, sump pit continues to rotate clockwise and be staggered with equal liquid hole, at this time pipe to fluid apertures and corresponding cylinder to fluid apertures according to
So do not reach connected state, if then continuing turn rotation pipe clockwise, pipe can be allowed to be connected to fluid apertures with corresponding cylinder to fluid apertures；
Example 2：In the circumferential direction of rotation pipe, equal liquid hole, sump pit, pipe to fluid apertures, cylinder to fluid apertures along clockwise direction successively
Arrangement, equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）When operation, row
Liquid bath is rotated counterclockwise with rotation pipe to alignment equal liquid hole（The process middle pipe is not connected to corresponding cylinder to fluid apertures to fluid apertures）,
After the completion of hydraulic control amount, sump pit is rotated clockwise and is staggered with equal liquid hole, and pipe does not have still with corresponding cylinder to fluid apertures to fluid apertures at this time
Have and reach connected state, if then continuing turn rotation pipe clockwise, pipe can be allowed to be connected to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber and culture vessel：Quantitative intracavitary is equipped with tune amount liquid capsule 34, and tune amount liquid capsule is equipped with and is used for
To the liquid supply pipe of tune amount liquid capsule feed flow, liquid supply pipe one end is connected to tune amount liquid capsule, and liquid supply pipe is by being in the hard tube section of quantitative intracavitary
And the outer segment being in outside quantitative chamber is constituted；
Pedestal is used for liquid supply pipe feed flow equipped with several for fluid cylinder 5, includes cylinder body 5a for fluid cylinder, close with cylinder body sliding
It seals the feed flow piston 5b of cooperation and is connect with feed flow piston and be in the piston rod 5c above feed flow piston, feed flow piston is slided
Dynamic direction is upper and lower directions, at one in fluid cylinder：Space in cylinder body below feed flow piston is sap cavity 5d, and feed flow is lived in cylinder body
Square space is adjusting cavity 5e beyond the Great Wall；
Sap cavity is corresponded with liquid supply pipe, and the liquid supply pipe other end is connected to corresponding sap cavity, and liquid supply pipe passes through rotation pipe side
Wall and rotation pipe lower end seal between liquid supply pipe and rotation pipe side wall and fix.
The pedestal is equipped with to be equipped with horizontally disposed adjustable plate 61, lives with the fixed adjustment seat 6 of pedestal, adjustment seat lower section
Stopper rod passes through adjustable plate, and equipped with upper holder block 611 and lower drawing block 612, adjustment seat is equipped with vertical arrangement and across tune piston rod
The homophony pole 62 of seat is saved, homophony pole is threadedly coupled with adjustment seat, and homophony pole is equipped with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, is equipped at the top of homophony pole fixed with homophony pole
Main regulation knob 621.
The homophony pole is equipped with the instruction disk 622 being in above adjustment seat, indicates that disk and homophony pole are coaxial, adjusts
Seat is equipped with the indicating arm 63 of slot with a scale, and indicating arm is arranged vertically and indicating arm contact instruction disk side wall.
As previously mentioned, the present invention can be to carrying out equivalent while liquid feeding in each ware body.And in the present solution, can also be right
Liquid volume added is regulated and controled.At the beginning, tune amount liquid capsule is vacuum, if specified rate chamber is full of at this time, liquid volume added is exactly the reality of quantitative chamber
Border volume.It, can be with turn homophony pole when needing to control liquid volume added（Main regulation knob）, homophony pole is made to move upward or downward
It is dynamic, for moving down：Homophony pole drives adjustable plate to move down vertically by the upper holder block on homophony pole at this time（Upper holder block
Rotation, adjustable plate do not turn）, adjustable plate pushes down on each feed flow piston, the liquid in each sap cavity by each lower drawing block on piston rod
Body（Can be water or other liquid, as long as in stable condition, will not be damaged to structures such as the pipelines of process）Injection pair
The tune amount liquid capsule answered, at this point, the actual volume of quantitative chamber（Actual volume refers to accommodate the volume of culture solution）Become smaller,
And the numerical value specifically to become smaller, it can be read from indicating arm（Scale slot on indicating arm can mark and the practical appearance of quantitative chamber
The corresponding number of product, digital occurrence can be converted when manufacturing and designing with measured in advance.Bid can be done on instruction disk
Note, convenient for the reading being directly aligned come mark-sense according to label, alternatively, instruction disk itself can also be used as label to make
With, such as using instruction disk top surface or bottom surface be used as label）.The above process can become smaller the actual volume of quantitative chamber, to
Control liquid volume added（Make its reduction）；Conversely, homophony pole is allowed to move up, then the liquid in tune amount liquid capsule can be extracted out, to
Controllable liquid volume added（Make its increase）.It is noted that liquid supply pipe is by being in the hard tube section of quantitative intracavitary and being in outside quantitative chamber
Outer segment constitute, wherein outer segment cannot be perfect rigidity, to ensure that diaphragm plate, core pipe etc. being capable of normal rotations（Core pipe
It is connect with outer segment, when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity）, outer segment can
To select the pipe of the types such as sebific duct, oil cylinder petroleum pipeline.
The sealing compression bar 7 arranged vertically is equipped in the rotation pipe, sealing compression bar passes through all diaphragm plates and sealing compression bar
It is threadedly coupled between all diaphragm plates, sealing compression bar is equipped with several pressure plates 71 for compressing hole sealing plate, pressure plate
It is corresponded with hole sealing plate, pressure plate is in the top of corresponding hole sealing plate.As previously mentioned, filling it up with liquid in rotation pipe, then seal
After orifice plate seals even lumen pore, separate between each quantitative chamber.But, in order to allow hole sealing plate want can the thorough company's of sealing lumen pore, hole sealing plate
Mutual extruding between one piece of diaphragm plate of lower contact is inevitable larger.After being used for multiple times, it is easy to cause because abrasion is larger
Sealing is insufficient.And in the present solution, hole sealing plate seals even after lumen pore, compression bar can be sealed with turn, and pressure plate is driven to move down and press
Tight corresponding hole sealing plate, further increases leakproofness, ensures the partition effect between each quantitative chamber.And there are sealing compression bar, pressure
After tight plate, the too tight of pressure is not had to during fabrication, between hole sealing plate and diaphragm plate（Leakproofness can be protected by the pushing of pressure plate
Barrier）, so as to effectively reduce abrasion, prolong the service life.It is noted that after having sealing compression bar, pressure plate, turn allowing
Before the motionless and turn core pipe of dynamic pipe, pressure plate should be allowed to be not located at the state for compressing corresponding hole sealing plate, otherwise will appear " turning motionless "
The case where.
Claims (6)
1. a kind of cell culture device for cultivating, measuring and analyzing, characterized in that including pedestal and several upper end openings
Culture vessel, each culture vessel stack gradually from the bottom to top, and a culture vessel of bottom contacts pedestal, is set on the pedestal
There are fixed upright bar, culture vessel lateral wall to be equipped with the retaining collar being adapted to fixed upright bar, retaining collar is set in fixed vertical
On bar, culture vessel includes ware body, the column in ware body on bottom is arranged and is arranged on ware body sidewall and is in tilted layout
Liquid injection pipe, column upper/lower terminal are open, and a rotation pipe arranged vertically passes through all columns, the rotation pipe and column
It can relatively rotate, and in sealing contact between rotation pipe lateral wall and column madial wall, rotation pipe lateral wall is equipped with through rotation
The sump pit of pipe upper/lower terminal, in a culture vessel：Column is equipped with the equal liquid hole being connected to inside ware body, equal liquid hole
It is interior to be equipped with cell filtering net, it is connected to inside liquid injection pipe lower end and ware body；
It is equipped with core pipe in the rotation pipe and several horizontally disposed diaphragm plates, core pipe lower end are stretched into the through-hole of bottom, covered in core pipe
Equipped with support ring, support ring is higher than core pipe bottom and support ring is in the through-hole of bottom, and support base, support base top are equipped in the through-hole of bottom
The annular groove that portion is equipped with seat slot and is adapted to rotation pipe lower end, support ring bottom contact seat slot slot bottom, and annular groove is stretched into rotation pipe lower end
Interior and rotation bottom of the tube contacts annular groove slot bottom, and rotation pipe is rotatably assorted with support base, and core pipe is across support base and core pipe and support
Seat is rotatably assorted, and diaphragm plate is set in core pipe, and diaphragm plate cross section is in circular ring shape, and rotation pipe, core pipe and each diaphragm plate are same
Axis, core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, be sealed between diaphragm plate and rotation pipe solid
It is fixed；
Diaphragm plate is equipped with even lumen pore, and core pipe is equipped with several hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One corresponds to, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts diaphragm plate top
Face；
Quantitative chamber is formed in rotation pipe between adjacent two pieces of diaphragm plates, if rotation pipe is equipped with main pipe to fluid apertures, quantitative chamber and pipe
Fluid apertures one-to-one correspondence, quantitative chamber is given to be corresponded with culture vessel, in corresponding quantitative chamber and culture vessel：Column is equipped with
Cylinder gives fluid apertures, quantitative chamber to be connected to cylinder to fluid apertures to fluid apertures by corresponding pipe, and pipe is cylinder to fluid apertures with cylinder to fluid apertures,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest point is higher than cylinder to fluid apertures
Lowest point, cylinder are higher than equal liquid hole highest point to fluid apertures lowest point.
2. a kind of cell culture device for cultivating, measuring and analyzing according to claim 1, characterized in that described
Pedestal includes matrix and several support columns being arranged below matrix, and matrix, which is equipped with, runs through matrix bottom through-hole upper and lower surfaces of,
Rotation pipe lower end is stretched into the through-hole of bottom, and the opening up fluid cylinder that connects is equipped with below the through-hole of bottom, be equipped with below matrix with it is at least one
The bottom bracing plate of support column connection, bottom bracing plate be equipped with the cylinder slot that connects fluid cylinder bottom and be adapted to, connect fluid cylinder bottom and be in a slot.
3. a kind of cell culture device for cultivating, measuring and analyzing according to claim 1, characterized in that right
In the quantitative chamber and culture vessel answered：Quantitative intracavitary is equipped with tune amount liquid capsule, and tune amount liquid capsule is equipped with for giving tune amount liquid capsule feed flow
Liquid supply pipe, liquid supply pipe one end is connected to tune amount liquid capsule, and liquid supply pipe is by being in the hard tube section of quantitative intracavitary and being in outside quantitative chamber
Outer segment constitute；
Pedestal is used for liquid supply pipe feed flow equipped with several for fluid cylinder, is included cylinder body for fluid cylinder, is slidingly sealed cooperation with cylinder body
Feed flow piston and the piston rod that connect and be in above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, at one in fluid cylinder：Space in cylinder body below feed flow piston is sap cavity, the space in cylinder body above feed flow piston
For adjusting cavity；
Sap cavity and liquid supply pipe correspond, and the liquid supply pipe other end is connected to corresponding sap cavity, liquid supply pipe across rotation pipe side wall and
Rotation pipe lower end seals between liquid supply pipe and rotation pipe side wall and fixes.
4. a kind of cell culture device for cultivating, measuring and analyzing according to claim 3, characterized in that described
Pedestal is equipped with is equipped with horizontally disposed adjustable plate with the fixed adjustment seat of pedestal, adjustment seat lower section, and piston rod passes through adjustable plate,
Piston rod is equipped with upper holder block and lower drawing block, and adjustment seat is equipped with vertical arrangement and the homophony pole across adjustment seat, main regulation
Bar is threadedly coupled with adjustment seat, and homophony pole is equipped with upper holder block and lower drawing block, and the contact of upper holder block bottom surface adjusts plate top surface, drop-down
Block top surface contacts adjustable plate bottom surface, is equipped with and the fixed main regulation knob of homophony pole at the top of homophony pole.
5. a kind of cell culture device for cultivating, measuring and analyzing according to claim 4, characterized in that described
Homophony pole is equipped with the instruction disk being in above adjustment seat, indicates that disk and homophony pole are coaxial, adjustment seat is equipped with a scale
The indicating arm of slot, indicating arm is arranged vertically and indicating arm contact instruction disk side wall.
6. a kind of cell culture device for cultivating, measuring and analyzing according to claim 1 or 3 or 4, feature
It is, all diaphragm plates are passed through equipped with the sealing compression bar arranged vertically, sealing compression bar in the rotation pipe and seals compression bar and own
It is threadedly coupled between diaphragm plate, sealing compression bar is equipped with several pressure plates for compressing hole sealing plate, pressure plate and hole sealing plate
It corresponds, pressure plate is in the top of corresponding hole sealing plate.
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| CN201610708334.3A CN106244453B (en) | 2016-08-22 | 2016-08-22 | A kind of cell culture device for cultivating, measuring and analyzing |
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| CN201610708334.3A CN106244453B (en) | 2016-08-22 | 2016-08-22 | A kind of cell culture device for cultivating, measuring and analyzing |
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| CN106244453B true CN106244453B (en) | 2018-08-24 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106467893B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment auxiliary device |
| CN106282017B (en) * | 2016-08-22 | 2018-09-21 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106222089B (en) * | 2016-08-22 | 2018-09-21 | 王铁苗 | A kind of experiment auxiliary culture apparatus |
| CN106047703B (en) * | 2016-08-22 | 2018-08-10 | 青岛爱博生物科技有限公司 | A kind of test sample culture apparatus |
| CN106222087B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of sample culture carrier |
| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
| CN106222090B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment assistant carrier |
| CN106244457B (en) * | 2016-08-22 | 2018-08-03 | 日照安维信生物科技有限公司 | A kind of experiment culture carrier structure |
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Address after: 311200, Zhejiang province Hangzhou city Xiaoshan District Xiaoshan science and technology city B block 3 floor Rong Ze biology Patentee after: Hangzhou Rongze Biotechnology Group Co.,Ltd. Address before: 311200, Zhejiang province Hangzhou city Xiaoshan District Xiaoshan science and technology city B block 3 floor Rong Ze biology Patentee before: HANGZHOU RONGZE BIOTECHNOLOGY Co.,Ltd. |