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CN105998752B - A kind of Chinese medicine composition for the treatment of of vascular dementia - Google Patents

A kind of Chinese medicine composition for the treatment of of vascular dementia Download PDF

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CN105998752B
CN105998752B CN201610565191.5A CN201610565191A CN105998752B CN 105998752 B CN105998752 B CN 105998752B CN 201610565191 A CN201610565191 A CN 201610565191A CN 105998752 B CN105998752 B CN 105998752B
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vascular dementia
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ginseng
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CN105998752A (en
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李�浩
刘剑刚
韦云
刘美霞
裴卉
刘龙涛
郭明冬
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Xiyuan Hospital China Academy Of Chinese Medical Sciences
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    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The invention discloses a kind of Chinese medicine compositions for the treatment of of vascular dementia, including ginseng, Rhizoma Gastrodiae, Ramulus euonymi and Rhizoma Chuanxiong.The composition can be prepared into the Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia, it is that ginseng and Rhizoma Chuanxiong are subjected to alcohol extracting, Rhizoma Gastrodiae and Ramulus euonymi carry out water and mention, gained alcohol extract and water extract mixing, vacuum drying, crushing, directly take orally or other dosage forms such as granule, capsule, soft capsule, electuary, pill, soft extract are made etc..Composition of the invention is made of natural medicinal plant completely, hormone-free, does not also add other synthetics such as pigment, after continuous use, has no toxic and side effect to human body.The Chinese medicine composition contains the Multiple components such as the ginsenoside being easily absorbed by the body, chuanxingol, ligustrazine, gastrodin, Quercetin, it can be effective for the treatment of vascular dementia, increase cerebral blood flow (CBF), reduce cerebral vascular resistance, enhance immunity of organism activity, it is calming the liver with qi-restoratives intelligence development, the effect of disperse blood stasis and dredge collateral.

Description

A kind of Chinese medicine composition for the treatment of of vascular dementia
Technical field
The present invention relates to especially a kind of Chinese medicine compositions for the treatment of of vascular dementia.
Background technique
Vascular dementia (vascular dementia, VD) is one group of intelligence as caused by cranial vascular disease and cognition function Energy distress syndrome, is one of common disease of senile dementia.VD morbidity is more clear, has potentially prevention and treatment property, again Referred to as " cataphrenia ", early diagnosis and early treatment improve or reverse especially pronounced for intelligence.It is old with population The disease incidence of age process, VD is in obvious ascendant trend, seriously threatens the health and life quality of the middle-aged and the old, to trouble Person, family and society bring long-term pain and burden, cause the extensive concern of society and medical profession.By 2010, generation The prevalence of dementia of over-65s crowd is 15% within the scope of boundary, wherein 9% is AD, 4% is VD.VD falls ill in stroke crowd Rate about 8.5%.And the prevalence survey of China VD is shown, 60 years old or more elderly dementia's illness rate is that 3.96%, VD accounts for 2/3, There are about 30% apoplexy patients to be likely to occur hypophrenia.Effective therapeutic agent is found, can be delayed in effectively preventing in early days Disease process, the quality of life for improving simultaneous phenomenon, improving patient, undoubtedly have important social reality meaning.
Different according to the cause of disease and pathology, the classification of VD mainly includes dementia with multiple cerebral infarction, multiple lacunar infarction It is dull-witted, massive cerebral infarction is dull-witted, binswanger disease (Binswanger disease), cerebral amyloid angiopathy are dull-witted, Dementia caused by dementia, hemorrhagic lesions caused by ischemic and Hypoxic Low perfusion syndrome etc..Diagnose vascular dementia, it is necessary to Have dementia symptom, history of stroke, transient ischemic attack history and focal nerve system sign, CT or MRI, which are checked, confirms brain Interior focal lesion；Dementia must take place at after stroke onset in 3 months, and dementia symptom can occur suddenly or slowly be in progress, The course of disease is in fluctuation or steps progress.
VD is the concept in modern medicine, although traditional medicine has earlier understanding to dementia, does not have so far to VD and is The discussion of system, description thereof are still dispersed in the diseases such as " slow-witted card ", " amnesia ", " forgetful ".China Association of Traditional Chinese Medicine's gerontology meeting VD is clearly incorporated into " Research of Senile Dementia Treated " in " diagnosis, differentiation of symptoms and signs for classification of syndrome and the efficacy assessment standard of Research of Senile Dementia Treated " that nineteen ninety formulates Scope.Traditional Chinese medicine sees " Plain Questions menstruation regulating opinion " cloud in relation to dull-witted discussion earliest: " blood and under, gas and in upper are disorderly apt to Forget "；Jing-Yue Complete Works propose that dementia is independence disease for the first time, recognize it beginning and have a substantially frame, book neutrality have " it is demented, It is dull-witted " a special piece, it is provided that " dementia card, it is all usually without phlegm, and or with pent-up, or with unsuccessful, or to ponder over, or with terrified and gradually extremely Dementia, card is then very strange, penetrates everywhere ".It is totally spy with " slow-witted and stupid " although dull-witted symptom numerous and complicated Sign.Until the "guide to clinical practice with medical record" of clear YE Tian shi: " apoplexy in, the slow-witted enuresis of mind, old man are fainted obviously ".In explicitly pointing out Wind can cause intelligent decline.Contemporary, Shen Jin chela also has the note of " forgetful after apoplexy " in " miscellaneous diseases source stream rhinoceros candle apoplexy " Carry, everybody just gradually recognizes some type of dull-witted and apoplexy, and there is inner links, but in traditional Chinese medicine with other types Dementia does not make always stringent differentiation, therefore still together with other forms of dementia and claims " dementia ", " dementia " so far.Make a general survey of each learning handed down in a family Say, VD is asthenia in origin and asthenia in superficiality disease, this void is kidney essense, deficiency of qi and blood, actually phlegm is turbid for mark, hemostasis, turbid poison individually or mutually tie it is stagnant in Train of thought, this void influence each other in fact with mark, mutually convert, form the pathological state of vicious circle, above disturb clear key and cause brains dystrophy, clearly Spirit is disturbed and sends out as dementia.
With the increase of the aged, the prevention and treatment problem of vascular dementia has become that domestic and international medical field is of interest to ask Topic, modern medicine there is no ideal medicament at present.Therefore, TCM Features and advantage are played, this disease is made effective Diagnosis, prevention and remedy measures treat this global problem to senile dementia is solved, have important clinical value.
The Chinese patent of publication number CN101683368A (publication date 2010.03.31) discloses a kind of Chinese medicine composition, packet Rhizoma Chuanxiong, Radix Angelicae Sinensis and natural borneol are included, have the function of anti-stroke and its sequelae, improve cardiac and cerebral blood-supply etc..
The Chinese patent of publication number CN101856468A (publication date 2010.10.13), which discloses, a kind of treats senile dementia Pharmaceutical composition, including the total glycosides of Rhizoma Gastrodiae and uncaria total alkaloids have Post stroke multiple infarct dementia certain treatment Effect.
Summary of the invention
In order to efficiently and effectively prevent and treat vascular dementia disease, suffer from especially for vascular senile dementia The symptoms such as person's early memory power decline, the present invention provides a kind of Chinese medicine compositions for the treatment of of vascular dementia, can effectively mention The abilities such as height is directed toward memory, associative learning, image is recalled, random shape is re-recognized and portrait is recalled.
The Chinese medicine composition for the treatment of of vascular dementia provided by the invention, including ginseng, Rhizoma Gastrodiae, Ramulus euonymi and Rhizoma Chuanxiong.
With ginseng be monarch in side, have reinforce vital energy, calming the nerves increases the function of intelligence, be to be directed to assertive evidence qi deficiency to blood stasis and set；Rhizoma Gastrodiae There is suppressing hyperactive liver for calming endogenous wind, nourishing and suppressing Yang for minister, first the function of blood circulation puts down its high upper sun, first prevents the change of its positive highization wind, There is the meaning of " preventiveing treatment of disease "；Ramulus euonymi be assistant have promoting circulation of blood dredging collateral, dissipate the stasis of blood function, can weeding through the old to new, restore crystal balance, make ginseng It is active smooth flourish greasy without stopping up.Rhizoma Chuanxiong is to make simultaneous guiding drug, has activating microcirculation and removing stasis medicinal, and the function of promoting the circulation of qi is the gas medicine in blood, can lead all medicines Directly enter blood system, and benefaction head, all medicines can be carried and directly enter brain key；All medicines share plays qi-restoratives intelligence development, calming the liver, disperse blood stasis and dredge collateral altogether Function.
Preferably, the Chinese medicine composition of above-mentioned treatment of vascular dementia, according to parts by weight, the dosage of each component are ginseng 6~12 parts, 6~12 parts of Rhizoma Gastrodiae, 6~12 parts of Ramulus euonymi, 3~9 parts of Rhizoma Chuanxiong.
Preferably, the Chinese medicine composition of above-mentioned treatment of vascular dementia, according to parts by weight, the dosage of each component are ginseng 9 parts, 9 parts of Rhizoma Gastrodiae, 9 parts of Ramulus euonymi, 6 parts of Rhizoma Chuanxiong.
The present invention also provides a kind of Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia, use any description above Chinese medicine composition is prepared by the following method:
Ginseng and Rhizoma Chuanxiong mixing, add 70% (v/v) ethanol water refluxing extraction of 8 times of weight, collect leachate, then 70% (v/v) the ethanol water refluxing extraction for adding 4 times of weight into ginseng and Rhizoma Chuanxiong again, collects leachate, two-stage leaching liquid Mixing concentration, obtains alcohol extracting thick paste；
Rhizoma Gastrodiae and Ramulus euonymi mixing, add the water of 10 times of weight to decoct, filter, the water for adding 8 times of weight continues to decoct, mistake Filter, merges filtrate twice, and water is obtained after reduced pressure and mentions thick paste；
Alcohol extracting thick paste and water are mentioned thick paste to merge, are dried in vacuo, is crushed.
The effective component Ligustilide of Rhizoma Chuanxiong, ligustrazine, phenolic acid (tanshin polyphenolic acid B, ferulic acid) are to vascular dementia There is preferable therapeutic effect, also there is obvious coronary artery dilator, increase coronary blood flow, relaxation vascular smooth muscle, drop The pharmacological actions such as low platelet surface-active.Ligustrazine, the extracting method of ferulic acid are higher using alcohol extracting content simultaneously.Prevention and treatment The ginseng active principle of vascular dementia is ginsenoside, and ginsenoside has extensive bioactivity, can improve the life of patient Bioplasm amount, extending life have good effect, and the alcohol extracting thing content of ginsenoside is higher, and ginseng and Rhizoma Chuanxiong are using above-mentioned The method of alcohol extracting being capable of effective component extracting to greatest extent.
Rhizoma Gastrodiae has the effect of arresting convulsion that relieves dizziness, high fever, infantile convulsions, epilepsy, etc., calming soporific, be mainly used for treatment have a dizzy spell, cold and headache, limbs fiber crops Wood, insomnia, infantile convulsion etc..The effective component of Rhizoma Gastrodiae is generally evaluated with Gastrodin, the total glycosides of Rhizoma Gastrodiae, and Gastrodin molecular weight is smaller, pole Property it is larger, adopt and be extracted with water with simple process, extract the higher advantage of content.Main component Quercetin, the winged euonymus of Ramulus euonymi It is relatively stable that alcohol water extracts content, so, it is more beneficial that Rhizoma Gastrodiae and Ramulus euonymi adopt the method being extracted with water.
It is highly preferred that the Chinese materia medica preparation bulk pharmaceutical chemicals of above-mentioned treatment of vascular dementia, ginseng and Rhizoma Chuanxiong first time return time It is 2.0 hours, second of return time is 1.5 hours.The return time that ethyl alcohol extracts is controlled at 2.0 and 1.5 hours, further Abundant effective component extracting.
It is highly preferred that when the Chinese materia medica preparation bulk pharmaceutical chemicals of above-mentioned treatment of vascular dementia, Rhizoma Gastrodiae and Ramulus euonymi decoct for the first time Between be 2.0 hours, second decocting time is 1.5 hours.The time of water boiling and extraction controls at 2.0 and 1.5 hours, further Abundant effective component extracting.
It is highly preferred that the Chinese materia medica preparation bulk pharmaceutical chemicals of above-mentioned treatment of vascular dementia, the temperature condition of the reduced pressure are 60℃.Temperature is excessively high to be easily destroyed effective component, and the too low concentration speed of temperature is slow, with 60 DEG C of optimums.
Above-described bulk pharmaceutical chemicals both can directly be taken, can also again by conventional formulations be prepared into it is a variety of its His dosage form, for example, pill, tablet, electuary, granule, capsule, soft capsule, pill, etc..
Compared with prior art, the invention has the following advantages:
Chinese medicine composition of the invention adds ginseng and Ramulus euonymi flavour of a drug, has the effect of qi-restoratives intelligence development, disperse blood stasis and dredge collateral. Ginseng mainly contains panaxoside and organic acid and esters, has to learning memory disorder and improves effect, while also having Play the role of it is antifatigue, significantly improve maincenter intend choline activity and quasi- catecholamine activity, choline system function can be enhanced, increase Cerebral blood flow (CBF) and brain energy metabolism.Addition Rhizoma Chuanxiong contains the main components such as Ligustilide, chuanxingol, ligustrazine and ferulic acid, has It is improved the effect of coronary flow, Central nervous system has apparent sedation.Ramulus euonymi contains Quercetin, dulcitol etc. Substance, (20%) containing saturated fatty acid in seed oil have regulating lipid metabolism and mitigate atherosclerosis (AS) lesion journey The effect of degree.And the vascular dementia mainly cognition dysfunction syndrome as caused by cranial vascular disease, therefore this medicine group At the cognition dysfunction for improving patient while more targetedly, preventing and treating cerebrovascular.Several herbal medicines are shared with comprehensive Prevention and treatment cranial vascular disease is closed, the cognitive function of patient is improved.
The technique that bulk pharmaceutical chemicals use conventional method and modernism to combine, it is easy to operate, while effective component maximum limit Degree retains.
Specific embodiment
In order to enable those skilled in the art to better understand the solution of the present invention, With reference to embodiment to this hair It is bright to be described in further detail.
Embodiment 1 joins the preparation of numb Yizhi Decoction
9 parts of ginseng, 9 parts of Rhizoma Gastrodiae, 9 parts of Ramulus euonymi, 6 parts of Rhizoma Chuanxiong are weighed by weight.
The technique preparation of drug is combined using conventional method and modernism, and specific processing is as follows:
Ginseng, Rhizoma Chuanxiong prepare mixing in proportion, using adding 70% (v/v) ethanol water refluxing extraction 2 of 8 times of weight small When, leachate is collected, 70% (v/v) ethanol water of 4 times of weight is then added into remaining ginseng Rhizoma Chuanxiong mixture again Refluxing extraction 1.5 hours, leachate is collected, two-stage leaching liquid merges, is concentrated under reduced pressure into paste, obtains alcohol extracting thick paste.
Rhizoma Gastrodiae, Ramulus euonymi prepare mixing in proportion, are extracted by the way of adding water to cook in tank, first plus the water of 10 times of weight The water that decoction 2.0 hours, filtering, remaining Rhizoma Gastrodiae and Ramulus euonymi add 8 times of weight continues to decoct 1.5 hours, and filtering merges two Secondary filtrate, 60 DEG C are concentrated under reduced pressure into paste, obtain water and mention thick paste.
Add the amount of ethanol water for the second time, and the multiple of the amount of water is added to calculate by radix of bulk pharmaceutical chemicals ability weight, belongs to In calculation method generally in the art.
Above-mentioned alcohol extracting thick paste and water are mentioned thick paste to merge, vacuum drying obtains dry extract, crushes.Products obtained therefrom is named as ginseng Numb Yizhi Decoction.
Embodiment 2 joins the toxicological study of numb Yizhi Decoction
Join numb Yizhi Decoction side's acute toxicity testing (maximum dosage-feeding experiment)
1. experimental animal selects 4 week old cleaning grade ICR mouse 40, half male and half female, weight 20g~28g is tieed up purchased from Beijing Experimental animal Technology Co., Ltd., tonneau China, quality certification number: SCXK (capital) 2011-0004.It is fed in clean laboratory animal room Adaptation is tested after a week.
2. joining numb Yizhi Decoction liquid extract, raw drug content 4.00g/g, by China TCM Academy of Sciences Xiyuan Hospital Chinese materia medica preparation Room prepares (preparation method is shown in embodiment 1).The medical fluid of the 2.00g/ml of concentration containing crude drug is configured to before test with distilled water.
3. grouping and administration, 40 mouse are divided into 2 groups according to the method for random (applying SPSS software), i.e., control group and Administration group, every group 20, half male and half female.No. 16 stomach-filling syringe needles of administration group, for 24 hours in the maximum that can once be born with intragastric administration on mice Volume (0.4ml/10g weight) gastric infusion.Fasting 12h before stomach-filling can't help water, and conventional feeds after 4h are administered, and observe 14d, tire out It counts dosage 1120.0g crude drug/kg (about 170 times of quantity), control group gavages same volume distilled water, the same administration group of method.
4. observing mouse gross morphology appearance, behavioral activity, diet situation, excrement shape etc., and experiment note is carried out daily Record, is observed continuously in 4h, the morning, the afternoon of 2~14d is respectively observed 1 time after administration.Before daily administration and the 15d morning measures Mouse weight.Dissection dead animal in time observes organ disease situation, and the internal organs for being observed visually lesion is taken to carry out pathology system Piece inspection；Observation period terminates, and cervical dislocation puts to death all animals, and Systematic anatomy takes the internal organs for being observed visually lesion to carry out Pathology film-making inspection.
It is generally believed that according to the weight, maximum dosage-feeding is equivalent to 100 times of adult clinical daily dosage in mouse 1 day It is safer above.The result shows that 14d is observed continuously after administration, 20 mouse of administration group have no significant discomfort upon administration Or it is dead.The mouse state of mind is normal after administration, and hair color is white and glossy, and two is just normal.The execution of mouse cervical dislocation is gone forward side by side after 14d Row dissection, abdominal cavity, thoracic cavity, cranial cavity and main organs (heart, liver, spleen, lung, kidney) are showed no obvious macroscopic pathology and become Change.During test, survival mice body weight increase is normal, and through statistical analysis, mouse weight changes between two groups during observation Male and female there are no significant difference (P > 0.05).Therefore, it is safe and reliable for taking under routine dose.
Embodiment 3. joins the drug efficacy study of numb Yizhi Decoction
1, which joins numb Yizhi Decoction blood vessel blocking method, leads to the influence of VD
Pharmacodynamic study uses 4- blood vessel blocking method (4-VO method) VD model, from multiple links: (water maze is real for behaviouristics Test), Apoptosis and the effect of Elements research this medicine primary and secondary target spot such as controlling gene expression, inflammatory factor level, oxidative stress it is special Point and connection illustrate its multipath, the curative effect and mechanism of VD are intervened in multiple target point integration.
Animal packet: blank group gives isometric physiological saline；
Sham-operation group gives isometric physiological saline；
Model group gives isometric physiological saline；
Donepezil group, by 0.51g/d stomach-filling (stomach-filling Western medicine Doneppezil Hydrochloride, be equivalent to clinical equivalent dosage)；
Chinese medicine group, by 1.034g crude drug/d stomach-filling (being equivalent to clinical equivalent dosage)；
Group in Chinese medicine, by 5.17g crude drug/d stomach-filling (being equivalent to 5 times of clinical equivalent dosage)；
Big group of Chinese medicine, by 10.34g crude drug/d stomach-filling (being equivalent to 10 times of clinical equivalent dosage).
1.1 join influence of the numb Yizhi Decoction to treated rats in Morris water maze performance
Compared with normal group, model group either wear platform number, fourth quadrant distance or fourth quadrant time have it is aobvious It writes statistical difference (P < 0.01)；Rats in sham-operated group is without significant statistical difference (P > 0.05).Compared with model group rats, It is organized in donepezil group, Chinese medicine and either wears platform number, fourth quadrant distance or fourth quadrant time and have significant statistics Difference (P < 0.05 or P < 0.01), big group of Chinese medicine wear platform number, fourth quadrant distance has significant statistical difference (P < 0.05).Knot Fruit is shown in Table 1.
The comparison of 1. each group cognitive ability of rat of table
Group n Wear platform number (secondary) Fourth quadrant distance (cm) The fourth quadrant time (s)
Normal group 8 9.09±1.38 167.04±33.97 78.23±14.50
Sham-operation group 8 8.88±0.51 140.81±97.04 76.64±10.78
Model group 7 6.22±0.96■■▲▲ 73.97±22.48■■▲ 66.22±9.26■
Donepezil group 8 8.57±1.66●● 146.54±40.70● 77.56±12.44●
Chinese medicine group 7 7.25±1.56■■▲* 135.56±86.33 75.55±6.86
Group in Chinese medicine 8 7.59±0.79■▲● 157.43±65.09● 77.38±6.88●
Big group of Chinese medicine 7 7.64±1.54■▲● 142.13±47.07● 72.08±10.15
Note: compared with normal group,■P < 0.05,■■P < 0.01；Compared with sham-operation group,▲P < 0.05,▲▲P < 0.01； Compared with model group,●P < 0.05,●●P < 0.01；Compared with donepezil group,**P < 0.01
1.2, which join numb Yizhi Decoction, influences NOS, IL-1, TNF-α content in rat hippocampus
Compared with normal group, model group nitricoxide synthase (NOS), interleukin 1 (IL-1), tumor necrosis The factor-α (TNF-α) content has significant statistical difference (P < 0.01)；Rats in sham-operated group without significant statistical difference (P > 0.05).Compared with model group, the NOS content of each medication group has significant statistical difference (P < 0.05 or P < 0.01), each Chinese medicine The TNF-α content of intervention group has significant statistical difference (P < 0.01).It the results are shown in Table 2.
The comparison of NOS, IL-1, TNF-α content in 2. each group rat hippocampus of table
Group n NOS(U/ml) IL-1(ng/L) TNF-α(ng/L)
Normal group 8 6.84±0.90 0.179±0.066 0.217±0.058
Sham-operation group 8 6.88±0.81 0.186±0.038 0.223±0.057
Model group 7 9.36±0.94■■▲▲ 0.293±0.062■■▲▲ 0.664±0.080■■▲▲
Donepezil group 8 7.45±0.82●● 0.212±0.061● 0.312±0.124
Chinese medicine group 7 7.25±1.56●● 0.241±0.061 0.369±0.063■■▲▲●●
Group in Chinese medicine 8 7.71±1.58● 0.247±0.091 0.322±0.111■▲●●
Big group of Chinese medicine 7 7.64±1.54● 0.230±0.123 0.316±0.139●●
Note: compared with normal group,■P < 0.05,■■P < 0.01；Compared with sham-operation group,▲P < 0.05,▲▲P < 0.01； Compared with model group,●P < 0.05,●●P < 0.01；Compared with donepezil group,**P < 0.01.
1.3 join influence of the numb Yizhi Decoction to SOD, MDA and GSH, GSH-PX content in rat hippocampus
Compared with normal group, model group superoxide dismutase (SOD), malonaldehyde (MDA) and reduced glutathione (GSH), glutathione-peroxidase (GSH-PX) content has significant statistical difference (P < 0.01)；Rats in sham-operated group Without significant statistical difference (P > 0.05).Compared with model group, donepezil group SOD, MDA and GSH content has significant statistics It learns difference (P < 0.05 or P < 0.01)；Chinese medicine group MDA and GSH content have significant statistical difference (P < 0.05 or P < 0.01)； SOD, MDA and GSH content are organized in Chinese medicine significant statistical difference (P < 0.05 or P < 0.01)；Chinese medicine big group of SOD, MDA and GSH-PX content has significant statistical difference (P < 0.05 or P < 0.01).It the results are shown in Table 3.
The comparison of SOD, MDA and GSH, GSH-PX content in 3. each group rat hippocampus of table
Note: compared with normal group,■P < 0.05,■■P < 0.01；Compared with sham-operation group,▲P < 0.05,▲▲P < 0.01； Compared with model group,●P < 0.05,●●P < 0.01；Compared with donepezil group,**P < 0.01.
1.4 join influence of the numb Yizhi Decoction to the area CA1 Bcl-2 in rat hippocampus and Bax expression
Compared with normal group, -2 (B cell of the model group Hippocampal CA 1 apoptogene B cell lymphoma factor Leukemia2, Bcl-2) and -2 relevance X protein of the B cell lymphoma factor (Bcl-2associated X protein, Bax) Expression has significant statistical difference (P < 0.01)；Rats in sham-operated group is without significant statistical difference (P > 0.05).With model Group compares, and each medication intervention group Hippocampal CA 1 Bcl-2 and Bax expression have significant statistical difference (P < 0.05 or P < 0.01). It the results are shown in Table 4.
4. each group CA 1 Zone of Hippocampus in Mouse Bcl-2 of table is compared with Bax is expressed
Group n Bcl-2 (%) Bax (%)
Normal group 8 36.98±7.73 37.83±5.32
Sham-operation group 8 37.19±8.43 35.95±7.96
Model group 7 19.48±3.71■■▲▲ 60.92±10.7■■▲▲
Donepezil group 8 34.48±9.57●● 40.73±7.13●●
Chinese medicine group 7 32.48±6.21●● 42.48±9.94●●
Group in Chinese medicine 8 31.98±9.52●● 41.98±9.71●●
Big group of Chinese medicine 7 29.51±5.95● 42.36±10.32●●
Note: compared with normal group,■P < 0.05,■■P < 0.01；Compared with sham-operation group,▲P < 0.05,▲▲P < 0.01； Compared with model group,●P < 0.05,●●P < 0.01；Compared with donepezil group,**P < 0.01
2. joining the clinical research of numb Yizhi Decoction
Diagnostic criteria meet American Psychiatric Association phrenoblabia diagnosis handbook and statistic handbook standard (1994, the 4th Version, DSM- IV), US National neurological disease and stroke research institute and Neuroscience Research association, Switzerland (NINDS/AIREN) are made The vascular dementia diagnostic criteria ordered and neurology branch, Chinese Medical Association vascular dementia diagnostic criteria in 2002.In conjunction with Vascular dementia Chinese medical discrimination scale (the scale for thedifferentiation of of the formulations such as Tian Jinzhou in 1997 Syndromesof vascular dementia, SDSVD) vascular dementia TCM syndrome quantitative criteria is carried out, draft the VD void stasis of blood Hinder key, the Chinese medical discrimination draft of liver-yang upper harassing syndrome.
In June, 2012 to the test principle for using random, Oxiracetam control, non-pessimum between in December, 2015, it is included in Subject 65, from clinical symptoms, simple mental state checklist (MMSE), activities of daily living scale (ADL), serum Central cholinergic system etc. is studied, observe the light moderate Patients with Vascular Dementia of compound of Chinese herbal medicine clinical efficacy and Safety, 6 weeks as a treatment course, observes 2 courses for the treatment of altogether.63 parts of complete case history is filtered out after the course for the treatment of, and (2 patients are because cannot be hard Hold and take granule and fall off) test group (taking the numb Yizhi Decoction of ginseng) 31, control group (taking Oxiracetam) 32.Two groups of patients The no difference of science of statistics in terms of age, gender, course of disease isobase data, is comparable.
2.1 two groups of ADL integrals, MMSE integral contrasts
Comparison among groups, two groups of pretherapy and post-treatment ADL integrals, MMSE integrals are without significant statistical difference (P > 0.05)；In group Compare, has statistical difference (P < 0.05 or P < 0.01) before relatively treating after two groups of treatments, the results are shown in Table 5.
Table 5. two groups of ADL integrals, MMSE integral contrasts
Note: comparison among groups, compared with the control group,△P<0.05,△△P<0.01；In group relatively, compared with before treatment,#P< 0.05,##P<0.01。
The comparison of 2.2 two groups of MMSE curative effects
For two groups of MMSE projects, there is increased trend, only treatment group's directive force integral has aobvious before relatively treating It writes statistical difference (P < 0.05).It the results are shown in Table 6.
The comparison of 6. two groups of MMSE curative effects of table
Note: with table 5.
2.3 two groups of pretherapy and post-treatment disease total marks compare
Disease integration pretherapy and post-treatment for two groups compares, comparison among groups, compared with the control group without significant statistics before treatment Difference (P>0.05) has significant statistical difference (P<0.01) after treatment.Group in relatively, with treatment before compared with, two groups have it is aobvious It writes statistical difference (P < 0.01).It the results are shown in Table 7.
7. two groups of table pretherapy and post-treatment disease total marks compare
Note: with table 5.
The comparison of 2.4 two groups of Ach, AchE contents
Interior and control group ratio is organized in influence to patients serum's acetylcholine (Ach), acetylcholinesterase (AchE) content Compared with equal no difference of science of statistics (P>0.05) before treating, only Ach has significant statistical difference (P<0.05) after treatment.In group relatively, Compared with relatively, two groups have significant statistical difference (P < 0.01) to Ach, AchE content before treatment.It the results are shown in Table 8.
The comparison of 8. two groups of Ach, AchE contents of table
Note: with table 5.
Two groups of patients have carried out three big routines and hepatic and renal function inspection before and after treatment, do not find the exception of Index for examination Change.
The above description of the embodiments is only for helping to understand the core idea of the present invention.It should be pointed out that for this technology For the those of ordinary skill in field, without departing from the principle of the present invention, can also to medicine preparation of the invention into Row some improvements and modifications, these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims (5)

1. a kind of Chinese medicine composition for the treatment of of vascular dementia, which is characterized in that according to parts by weight, crude drug by 9 parts of ginseng, 9 parts of Rhizoma Gastrodiae, 9 parts of Ramulus euonymi, 6 parts of Rhizoma Chuanxiong compositions.
2. a kind of Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia, which is characterized in that use Chinese medicine group described in claim 1 Object is closed, is prepared by the following method:
Ginseng and Rhizoma Chuanxiong mixing, add 70% (v/v) ethanol water refluxing extraction of 8 times of weight, collect leachate, then again to In ginseng and Rhizoma Chuanxiong plus 70% (v/v) ethanol water refluxing extraction of 4 times of weight, collection leachate, two-stage leaching liquid mix Concentration, obtains alcohol extracting thick paste；
Rhizoma Gastrodiae and Ramulus euonymi mixing, add the water of 10 times of weight to decoct, filter, and the water for adding 8 times of weight continues to decoct, and filter, Merge filtrate twice, water is obtained after reduced pressure and mentions thick paste；
Alcohol extracting thick paste and water are mentioned thick paste to merge, are dried in vacuo, is crushed.
3. the Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia according to claim 2, which is characterized in that ginseng and Rhizoma Chuanxiong First time return time is 2.0 hours, and second of return time is 1.5 hours.
4. the Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia according to claim 2, which is characterized in that Rhizoma Gastrodiae and terrible arrow Plumage first time decocting time is 2.0 hours, and second of decocting time is 1.5 hours.
5. the Chinese materia medica preparation bulk pharmaceutical chemicals for the treatment of of vascular dementia according to claim 2, which is characterized in that the decompression is dense The temperature condition of contracting is 60 DEG C.
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