CN105779546A - Preparation method and application of intestinal membrane peptide - Google Patents
Preparation method and application of intestinal membrane peptide Download PDFInfo
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- CN105779546A CN105779546A CN201610296935.8A CN201610296935A CN105779546A CN 105779546 A CN105779546 A CN 105779546A CN 201610296935 A CN201610296935 A CN 201610296935A CN 105779546 A CN105779546 A CN 105779546A
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- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a preparation method and application of an intestinal membrane peptide. According to the method, the problems of great production odor, high salinity, high water content and single function of intestinal membrane stoste, namely intestinal membrane liquid I are solved. By adopting the steps of filtering the intestinal membrane stoste, adjusting a pH value, deodorizing, desalting and dehydrating a biological membrane, performing biological enzymolysis, drying, packaging products and the like, the content of small peptide in a produced intestinal membrane peptide product is greater than 50 percent (the molecular weight is smaller than or equal to 1000Da), and the product is easily digested and absorbed and can be used for promoting digestion and absorption of amino acid and mineral substances. The intestinal membrane peptide disclosed by the invention can be used for an animal feed additive and a functional protein raw material.
Description
Technical field
The present invention be directed to the recycling of agriculture field animal by-product processing waste resource goldbeater's skin liquid, particularly relate to preparation method and the application thereof of a kind of goldbeater's skin peptide.
Background technology
China's live pig aboundresources, is one of heparin sodium raw material and natural casing leading exporter, and current China mainly carries out heparin sodium extraction with intestinal mucosa for raw material.Heparin becomes complex with covalent bond form with protein bound in tissue, and heparin is disintegrated down by tradition mainly through salt solution and two kinds of approach of enzymatic isolation method.Extracting the high concentrated organic wastewater that the waste water after heparin is a kind of high salt, high nitrogen, desalination solution can directly obtain outside filtering residue intestine membrane protein, and the goldbeater's skin waste water of enzymatic isolation method is substantially directly with sewage discharge.It is reported reckoning, China's heparin waste water annual emissions about 700,000 tons, rough estimate containing crude protein quantity up to 4800 tons.These discharge of wastewater not only bring high risks to surrounding enviroment but also make a large amount of protein resources in short supply run off in vain.
The processing of goldbeater's skin waste water is mainly adopted wastewater collection enzymolysis by tradition, prepares the intestine membrane protein product rich in little peptide.This processing method Problems existing, one is that goldbeater's skin waste liquid is in the course of processing especially dry run, produce dense especially bad smell, extreme influence is brought to plant area's nearby residents life, can not get effective solution because stench abnormal flavour and cause goldbeater's skin process scale to be stagnated, therefore goldbeater's skin waste liquid does not obtain extensively effective recovery processing and utilizes；Two is the salinity often containing higher concentration in goldbeater's skin waste liquid, and after testing, in goldbeater's skin waste liquid dry, salt content is up to 45%, how to reduce salinity, improves the active constituent contents such as albumen and improves palatability etc., is never better solved；Three are machined to that this is higher, and in goldbeater's skin waste water, the overwhelming majority is water and salinity, and dry is only about 4.5%, is remained high by low concentration goldbeater's skin liquid drying cost.
Adopt the shortcoming that traditional method can not solve goldbeater's skin processing bad smell, high salinity, high drying cost very well, and the pig-breeding scale of China is still rising year by year, the processing capacity of heparin sodium and natural casing is also rising year by year, the goldbeater's skin waste liquid produced also will be continuously increased, therefore this field is in the urgent need to a kind of method providing goldbeater's skin stock solution deodorization, especially can reduce salinity and improve the process of product function.
Summary of the invention
It is an object of the invention to: preparation method and the application thereof of a kind of goldbeater's skin peptide are provided, the hydrogenperoxide steam generator under specified conditions is adopted to can be good at removing the bad smell of goldbeater's skin liquid I, simultaneously through test repeatedly, find the desalination that specific molecular biosciences film can be good, active constituent-enriched, and utilize the proteinase combination of special ratios, can well realize the biological enzymolysis of intestine membrane protein.
To achieve these goals, the present invention provides following technical scheme:
The preparation method of a kind of goldbeater's skin peptide, comprises the following steps:
(1) goldbeater's skin liquid I is collected: collects the waste residue and liquid after intestinal mucosa extracts heparin and filters, removes impurity, obtain goldbeater's skin liquid I；
(2) deodorize processes: goldbeater's skin liquid I keeps uniform temperature and pH value, after adding hydrogenperoxide steam generator reaction, obtains goldbeater's skin liquid II；
(3) dewatering and desalting processes: goldbeater's skin liquid II adopts molecular biosciences film carry out dewatering and desalting process and obtains goldbeater's skin liquid III；
(4) enzymolysis: adopt mixing protease to carry out biological enzymolysis goldbeater's skin liquid III, obtain goldbeater's skin liquid IV；
(5) dry: goldbeater's skin liquid IV to be dried, obtains goldbeater's skin peptide product；
(6) product packaging molding.
Preferably, step (1) filters employing 20～50 mesh filter screen.
Preferably, the temperature that in step (2), deodorize processes is 30～40 DEG C, and pH value is 7.0～8.5, and the response time is 1～4 hour.
Preferably, the weight of the interpolation of the hydrogenperoxide steam generator described in step (2) is the 0.5～3.0% of goldbeater's skin liquid I weight；Adopting concentration when regulating pH value is the sodium hydroxide solution of 5～10%.
Preferably, step (3) Middle molecule biomembrane is the one in plate and frame, tubular type, spiral wound and hollow fiber form, and molecular biosciences membrane aperture is 100～200Da, and after concentration, goldbeater's skin liquid III is the 10～50% of goldbeater's skin liquid II volume.
Preferably, in step (4), hydrolysis temperature is 40～60 DEG C, and enzymolysis time is 3～10 hours, weight is goldbeater's skin liquid III weight the 0.1～0.5% of mixing protease interpolation.
Preferably, in step (4), mixing protease is (2～5) by mass ratio: (2～5): the animal protease of (1～2), alkaline protease, papain form.
Preferably, in step (5), dried forms is the one in drum-type drying, centrifugal spray drying, pressure spray dryer.
Preferably, in prepared goldbeater's skin peptide, peptide molecular weight is distributed as 300-900Da, ash≤20%, and wherein, the little peptide content of molecular weight≤1000Da is more than 50%.
Further, the application of a kind of goldbeater's skin peptide, can be used for animal feed additive, it may also be used for functional protein raw material.
The present invention has the advantage that compared to existing technology
1, the present invention adopts hydrogen peroxide oxidation deodorize to process, and is removed by stink, meets national requirements for environmental protection, it is ensured that production security and sustainability.
2, the present invention adopts molecular biosciences membrane technology, realize thickening and the desalination of goldbeater's skin stock solution in a mild condition, both saved drying cost, meet national energy-saving and reduce discharging policy idea, improve again the content of the effective ingredient such as albumen, peptide, it is achieved the raising of product quality simultaneously.
3, the present invention adopts mixed biologic protease hydrolyzed technology, makes the goldbeater's skin peptide produced easy to digest, it is possible to promote aminoacid and mineral absorption, promotes Protein retention, has the specific functions such as antibacterial and enhancing immunity simultaneously.
4, the goldbeater's skin peptide of the present invention is the completely new product of a low salt, high nutrition, low energy consumption, has wide market prospect, it is possible to create good economic benefit and social benefit.
Detailed description of the invention
The preparation method of a kind of goldbeater's skin peptide, comprises the following steps:
(1) goldbeater's skin liquid I is collected: collect the waste residue and liquid after intestinal mucosa extracts heparin, adopts 20～50 mesh filter screens to filter, removes impurity, obtain goldbeater's skin liquid I；
(2) deodorize processes: goldbeater's skin liquid I is maintained at temperature and is 30～40 DEG C and when pH value is 7.0～8.5, adds after hydrogenperoxide steam generator reacts 1～4 hour, obtains goldbeater's skin liquid II；Wherein, the weight of the interpolation of hydrogenperoxide steam generator is the 0.5～3.0% of goldbeater's skin liquid I weight, and adopting concentration when regulating pH value is the sodium hydroxide solution of 5～10%.
(3) dewatering and desalting processes: goldbeater's skin liquid II adopts molecular biosciences film carry out dewatering and desalting process and obtains goldbeater's skin liquid III；Wherein, molecular biosciences film is the one in plate and frame, tubular type, spiral wound and hollow fiber form, it is preferred to hollow fiber form, and molecular biosciences membrane aperture is 100～200Da, and after concentration, goldbeater's skin liquid III is the 10～50% of goldbeater's skin liquid II volume.
(4) enzymolysis: adopt mixing protease to carry out biological enzymolysis goldbeater's skin liquid III, obtain goldbeater's skin liquid IV；Wherein, hydrolysis temperature is 40～60 DEG C, enzymolysis time is 3～10 hours, weight is goldbeater's skin liquid III weight the 0.1～0.5% of mixing protease interpolation, mixing protease is (2～5) by mass ratio: (2～5): the animal protease of (1～2), alkaline protease, papain form.
(5) dry: being dried by goldbeater's skin liquid IV, obtain goldbeater's skin peptide product, wherein, dried forms is the one in drum-type drying, centrifugal spray drying, pressure spray dryer.
(6) product packaging molding.
In prepared goldbeater's skin peptide, peptide molecular weight is distributed as 300-900Da, ash≤20%, and wherein, the little peptide content of molecular weight≤1000Da is more than 50%.
The functional characteristic of goldbeater's skin peptide prepared by said method is as follows:
1, goldbeater's skin peptide biological activity peptide, has raising immunity of organisms, reduces cultivated animals young baby especially piglet diseases and occurs, thus improving survival rate.
2, goldbeater's skin peptide can promote the deposition of amino acid whose absorption and protein, little peptide and aminoacid have separate mechanism of absorption, it is to avoid the common antagonism absorbing site and produce of vying each other, thus promoting amino acid whose absorption, accelerates the synthesis of protein.When protein supplies with little peptide form completely, the infiltration rate of lysine is no longer affected by arginine.
3, goldbeater's skin peptide promotes the absorbing of mineral element, and accelerates lipid metabolism, rich in being prone to the little peptide of trophism that absorbs, thus improving cultivated animals daily gain.
4, goldbeater's skin peptide has good palatability, promoting cultivated animals appetite, thus improving the cultivated animals speed of growth, improving efficiency of feed utilization.
5, there is antioxidation, parahormone, class antibiotic effect.
6, there is the growth function that stimulates digestion.
The application of goldbeater's skin peptide prepared by said method
Choosing 200 piglets of 40 age in days, be randomly divided into 2 groups, matched group eats normal daily ration, and test group eats the daily ration adding goldbeater's skin peptide prepared by 3% the inventive method in normal daily ration, 30 days experimental periods.Test data is as shown in the table:
Showing that intestinal membrane powder can improve daily gain and the feed efficiency of piglet by testing, all there is not diarrheal dehydration phenomenon in all test group piglets, and 5 diarrhoea occurs in matched group, it was shown that goldbeater's skin peptide can reduce piglet diarrhea；Test group villi height is high with the ratio relatively matched group of Crypt depth, illustrates that goldbeater's skin Toplink promotes the absorption of nutrient substance and the growth of intestinal tissue.
Goldbeater's skin peptide provided by the invention is rich in being prone to the little peptide that absorbs, little peptide and aminoacid have separate mechanism of absorption, thus promoting amino acid whose absorption, accelerate the synthesis of protein, improve the cultivated animals speed of growth, improve efficiency of feed utilization, and goldbeater's skin comes from intestinal mucosa, good palatability, promoting cultivated animals appetite, goldbeater's skin peptide also has absorbing of promotion mineral element, accelerates lipid metabolism, thus reaching to improve cultivated animals daily gain, it is a kind of green, efficient, safe nutritional feed additive.
Goldbeater's skin peptide provided by the invention is rich in the biologically active peptide of vital movement regulatory function, there is raising cultivated animals immunity of organisms, there is antioxidation, parahormone, class antibiotic effect, stimulate digestion growth, reach to reduce ablactational baby pig disease to occur, improve survival rate, be a kind of new type functional protein raw materials.
The foregoing is only presently preferred embodiments of the present invention, it is not limited to the substantial technological context of the present invention, the substantial technological content of the present invention is broadly to be defined in the right of application, any technology entities that other people complete or method, if defined with the right of application is identical, also or the change of a kind of equivalence, all it is covered by being considered among this right.
Claims (10)
1. the preparation method of a goldbeater's skin peptide, it is characterised in that comprise the following steps:
(1) goldbeater's skin liquid I is collected: collects the waste residue and liquid after intestinal mucosa extracts heparin and filters, removes impurity, obtain goldbeater's skin liquid I；
(2) deodorize processes: goldbeater's skin liquid I keeps uniform temperature and pH value, after adding hydrogenperoxide steam generator reaction, obtains goldbeater's skin liquid II；
(3) dewatering and desalting processes: goldbeater's skin liquid II adopts molecular biosciences film carry out dewatering and desalting process and obtains goldbeater's skin liquid III；
(4) enzymolysis: adopt mixing protease to carry out biological enzymolysis goldbeater's skin liquid III, obtain goldbeater's skin liquid IV；
(5) dry: goldbeater's skin liquid IV to be dried, obtains goldbeater's skin peptide product；
(6) product packaging molding.
2. the preparation method of goldbeater's skin peptide according to claim 1, it is characterised in that filter employing 20～50 mesh filter screen in step (1).
3. the preparation method of goldbeater's skin peptide according to claim 1, it is characterised in that the temperature that in step (2), deodorize processes is 30～40 DEG C, and pH value is 7.0～8.5, and the response time is 1～4 hour.
4. the preparation method of goldbeater's skin peptide according to claim 1, it is characterised in that the weight of the interpolation of the hydrogenperoxide steam generator described in step (2) is the 0.5～3.0% of goldbeater's skin liquid I weight；Adopting concentration when regulating pH value is the sodium hydroxide solution of 5～10%.
5. the preparation method of goldbeater's skin peptide according to claim 1, it is characterized in that, step (3) Middle molecule biomembrane is the one in plate and frame, tubular type, spiral wound and hollow fiber form, molecular biosciences membrane aperture is 100～200Da, and after concentration, goldbeater's skin liquid III is the 10～50% of goldbeater's skin liquid II volume.
6. the preparation method of goldbeater's skin peptide according to claim 1, it is characterised in that in step (4), hydrolysis temperature is 40～60 DEG C, enzymolysis time is 3～10 hours, weight is goldbeater's skin liquid III weight the 0.1～0.5% of mixing protease interpolation.
7. the preparation method of goldbeater's skin peptide according to claim 1, it is characterized in that, in step (4), mixing protease is (2～5) by mass ratio: (2～5): the animal protease of (1～2), alkaline protease, papain form.
8. the preparation method of goldbeater's skin peptide according to claim 1, it is characterised in that in step (5), dried forms is the one in drum-type drying, centrifugal spray drying, pressure spray dryer.
9. the preparation method of the goldbeater's skin peptide according to claim 1-8 any one, it is characterised in that in prepared goldbeater's skin peptide, peptide molecular weight is distributed as 300-900Da, ash≤20%, and wherein, the little peptide content of molecular weight≤1000Da is more than 50%.
10. the application of a goldbeater's skin peptide, it is characterised in that can be used for animal feed additive, it may also be used for functional protein raw material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610296935.8A CN105779546A (en) | 2016-05-06 | 2016-05-06 | Preparation method and application of intestinal membrane peptide |
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| CN201610296935.8A CN105779546A (en) | 2016-05-06 | 2016-05-06 | Preparation method and application of intestinal membrane peptide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520881A (en) * | 2016-12-10 | 2017-03-22 | 广州市嘉仁高新科技有限公司 | Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines |
| CN108260707A (en) * | 2016-12-30 | 2018-07-10 | 上海杰隆生物制品股份有限公司 | A kind of preparation method of animal dried porcine saluble |
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| CN103665192A (en) * | 2012-09-17 | 2014-03-26 | 什邡市乐励馥阳生物有限责任公司 | Method for extracting heparin sodium from small intestine of pig and co-producing protein powder |
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2016
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Patent Citations (4)
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| CN101703146A (en) * | 2009-10-30 | 2010-05-12 | 华南理工大学 | Method for purifying food-grade corn protein powder |
| CN102604747A (en) * | 2012-03-12 | 2012-07-25 | 南宁创新科技医药技术有限公司 | Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas |
| CN103665192A (en) * | 2012-09-17 | 2014-03-26 | 什邡市乐励馥阳生物有限责任公司 | Method for extracting heparin sodium from small intestine of pig and co-producing protein powder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520881A (en) * | 2016-12-10 | 2017-03-22 | 广州市嘉仁高新科技有限公司 | Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines |
| CN108260707A (en) * | 2016-12-30 | 2018-07-10 | 上海杰隆生物制品股份有限公司 | A kind of preparation method of animal dried porcine saluble |
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