CN105600943A - Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater - Google Patents
Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater Download PDFInfo
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- CN105600943A CN105600943A CN201511000061.9A CN201511000061A CN105600943A CN 105600943 A CN105600943 A CN 105600943A CN 201511000061 A CN201511000061 A CN 201511000061A CN 105600943 A CN105600943 A CN 105600943A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention provides a method using microorganism to effectively reduce BOD and COD levels in fermented wastewater, which comprises: performing composite culture on a composite strain in a culture medium to obtain composite strain liquid, wherein the composite strain comprises a combination of brewer's yeast, streptococcus lactis and rhodopseudomonas palustris; inoculating the composite strain liquid into a mixed system of the fermented wastewater and molasses, fermenting hermetically and finishing fermenting till pH is reduced to 3.5. By using the synergetic action of the microorganism, C and N elements in the wastewater are converted and used by beneficial bacterium in the composite microorganism, thereby effectively reducing the BOD and COD levels in the fermented wastewater; an applicant also finds that after the processing by the method, the obtained processing liquid can be further developed and used and the method is a very simple method capable of effectively reducing the BOD and COD levels in fermented wastewater.
Description
Technical field
The present invention relates to sewage treatment area, relate in particular to a kind of method of microbiological treatment fermentation waste water, enter oneStep ground is a kind of method of utilizing microorganism effectively to reduce BOD in fermentation waste water, COD level.
Background technology
Fermentation waste water, as a kind of sewage, wherein contains ammonia nitrogen, phosphorus, multiple enzyme, residual that fermentation producesThe material such as fermentation medium and a small amount of statoblast, do not meet " Shanghai City integrated wastewater discharge standard ".At present, large-scale fermentation enterprise is all furnished with corresponding sewage treatment facility, produces the fermentation waste water warp that fermentation producesCross several complicated treatment process, could discharge until up to standard.
Existing sewage disposal technology, according to degree for the treatment of, can be divided into coagulation, two stage treatment and three grades of placesReason. Wherein, coagulation is mainly removed the solid pollution material that is suspended state in sewage, general physics placeReason method major part can complete the requirement of coagulation, in fact, also can only complete the requirement of coagulation,Through the sewage of coagulation, BOD generally can remove 30% left and right, also can not reach final row far awayPut standard, can think that coagulation is only the pretreatment of two stage treatment. Two stage treatment is mainly removed in sewageBe the organic pollution (BOD, COD material) of colloid and dissolved state, utilization be biomembrance process, lifeThe methods such as thing edman degradation Edman, activated sludge process, wherein clearance can reach 90% left and right, thereby makes organic dirtDye thing and reach the discharge standard of walk-through. Tertiary treatment be mainly the organic matter of processing further difficult degradation, nitrogen andPhosphorus etc. can cause the solubility inorganic matter of body eutrophication etc., and main method has biological degradation method, coagulation to sinkShallow lake method, sand coarse aggregate ratio method, active carbon adsorption, ion-exchange and electric osmose analytic approach etc. Visible, sewage is completeCompletely be disposed wholely, make it to reach discharge standard, need appreciable equipment, operation and expense etc.
Therefore, a kind of simpler and easy, convenient, method of effectively processing fermentation waste water, is current this area skillArt personnel in the urgent need to.
Summary of the invention
The defect existing in actual application for above-mentioned background technology and prior art, the invention providesA kind of method of utilizing microorganism effectively to reduce BOD in fermentation waste water, COD level, compound by utilizingThe effect that microorganism is worked in coordination with mutually, makes C in waste water, N element by the beneficial bacterium in complex microorganism selfTrans-utilization, thus effectively reduce BOD, the COD level in fermentation waste water, moreover, the applicationInventor also finds, passes through as after the method processing, and the treatment fluid obtaining can be further for opening againSend out and utilize. Method provided by the present invention does not need the input of specific equipment and high energy consumption, is very letter of oneJust effectively reduce the method for BOD in fermentation waste water, COD level.
Technical scheme of the present invention comprises that one utilizes microorganism effectively to reduce BOD in fermentation waste water, COD waterFlat method, is characterized in that, comprises the following steps:
Step 1: composite bacteria is carried out in culture medium to compound cultivation, obtain composite bacteria liquid, wherein, described inComposite bacteria is the combination of saccharomyces cerevisiae, streptococcus lactis, Rhodopseudomonas palustris;
Step 2: described composite bacteria liquid is inoculated in the mixed system of fermentation waste water and molasses, sealed fermenting,Until pH is down to 3.5, finish fermentation.
In one embodiment of the invention, in described step 1, described saccharomyces cerevisiae (SaccharomycesCerevisiae) bacterial classification that is ACCC20065 for deposit number;
The bacterial classification that described streptococcus lactis (StreptococcusLactis) is ACCC10653 for deposit number;
Described Rhodopseudomonas palustris (Rhodopseudomonaspalustris) for deposit number isThe bacterial classification of ACCC10649.
The weight proportion of composite bacteria and culture medium is 1-10:90-99.
In one embodiment of the invention, also comprise the cultivation of bacterial classification, specifically comprise:
To in S. cervisiae bacterial classification access potato glucose fluid nutrient medium, carry out shaken cultivation, wherein, trainingFoster temperature is 20-35 DEG C, and the revolution of shaken cultivation is 180-220rpm, and incubation time is 20-48h, treats culture mediumAfter muddy, take out, put into 2-4 DEG C of Refrigerator store stand-by;
Streptococcus lactis strain and Rhodopseudomonas palustris bacterial classification are accessed respectively in nutrient broth fluid nutrient mediumRow shaken cultivation, wherein, cultivation temperature is 30-38 DEG C, the revolution of shaken cultivation is 180-220rpm, trainingThe foster time is 24-48 hour, after culture medium muddiness, takes out, and puts into 2-4 DEG C of Refrigerator store stand-by.
Wherein, through the incubation step of described bacterial classification, obtain 200,000,000/ml saccharomyces cerevisiae, 500,000,000/ml breast hammerBacterium and 0.6 hundred million/ml Rhodopseudomonas palustris, by according to the weight proportion of 20-25:40-44:31-40Mix, obtain composite bacteria.
Wherein, the component of described culture medium and weight proportion thereof preferably include: molasses 1-5 part, peptone 0-1Part, water 94-99 part, further, can add other suitable component as required on this basis.
The condition of described compound cultivation is: under 20-35 DEG C of condition, cultivate 100-150h, obtain described composite bacteria liquid.
In an embodiment of the present invention, in described step 2, the interpolation weight of fermentation waste water, honey, composite bacteria liquidAmount proportioning is 100-200:3-5:10-20.
The condition of described sealed fermenting is: sealed fermenting 5-10 days under 20-35 DEG C of condition, makes the pH of zymotic fluidBe down to 3.5. Now, stop fermentation, find that BOD, the COD value in fermentation waste water has all been reduced to and can have arrangedPut standard value.
Basis is bright utilizes useful complex microorganism to join in fermentation waste water, utilizes bacterium to the mesotrophic suction of waste liquidReceive, the nutrients accumulating can be decomposed into amino acid, lower fatty acid etc., and finally be degraded in fermentation waste waterInorganic matter, can effectively reduce COD in water, BOD value, makes fermentation waste water reach the standard of discharge. Warp simultaneouslyCross the fermentation waste water of processing and also can be used to manufacture the derived product such as liquid fertilizer, environmental purifying agent. The method is implementedMeans are very simple and easy, reduced actual processing cost concerning enterprise, increased economic benefit; For environmentProtection, has reduced discharge capacity, contributes to implement energy-saving and emission-reduction.
Detailed description of the invention
The invention provides a kind of side that utilizes microorganism effectively to reduce BOD in fermentation waste water, COD levelMethod, is characterized in that, comprises the following steps:
Step 1: composite bacteria is carried out in culture medium to compound cultivation, obtain composite bacteria liquid, wherein, described inComposite bacteria is the combination of saccharomyces cerevisiae, streptococcus lactis, Rhodopseudomonas palustris;
Step 2: described composite bacteria liquid is inoculated in the mixed system of fermentation waste water and molasses, sealed fermenting,Until pH is down to 3.5, finish fermentation.
In one embodiment of the invention, in described step 1, described saccharomyces cerevisiae (SaccharomycesCerevisiae) bacterial classification that is ACCC20065 for deposit number;
The bacterial classification that described streptococcus lactis (StreptococcusLactis) is ACCC10653 for deposit number;
Described Rhodopseudomonas palustris (Rhodopseudomonaspalustris) for deposit number isThe bacterial classification of ACCC10649.
The weight proportion of composite bacteria and culture medium is 1-10:90-99.
In one embodiment of the invention, also comprise the cultivation of bacterial classification, specifically comprise:
To in S. cervisiae bacterial classification access potato glucose fluid nutrient medium, carry out shaken cultivation, wherein, trainingFoster temperature is 20-35 DEG C, and the revolution of shaken cultivation is 180-220rpm, and incubation time is 20-48h, treats culture mediumAfter muddy, take out, put into 2-4 DEG C of Refrigerator store stand-by;
Streptococcus lactis strain and Rhodopseudomonas palustris bacterial classification are accessed respectively in nutrient broth fluid nutrient mediumRow shaken cultivation, wherein, cultivation temperature is 30-38 DEG C, the revolution of shaken cultivation is 180-220rpm, trainingThe foster time is 24-48 hour, after culture medium muddiness, takes out, and puts into 2-4 DEG C of Refrigerator store stand-by.
Wherein, through the incubation step of described bacterial classification, obtain 200,000,000/ml saccharomyces cerevisiae, 500,000,000/ml breast hammerBacterium and 0.6 hundred million/ml Rhodopseudomonas palustris, by according to the weight proportion of 20-25:40-44:31-40Mix, obtain composite bacteria.
Wherein, the component of described culture medium and weight proportion thereof preferably include: molasses 1-5 part, peptone 0-1Part, water 94-99 part, further, can add other suitable component as required on this basis.
The condition of described compound cultivation is: under 20-35 DEG C of condition, cultivate 100-150h, obtain described composite bacteria liquid.
In an embodiment of the present invention, in described step 2, the interpolation weight of fermentation waste water, honey, composite bacteria liquidAmount proportioning is 100-200:3-5:10-20.
The condition of described sealed fermenting is: sealed fermenting 5-10 days under 20-35 DEG C of condition, makes the pH of zymotic fluidBe down to 3.5. Now, stop fermentation, find that BOD, the COD value in fermentation waste water has all been reduced to and can have arrangedPut standard value.
Embodiment 1
Utilize microorganism effectively to reduce a method for BOD in fermentation waste water, COD level, comprise following stepRapid:
Step 1: will in S. cervisiae bacterial classification access potato glucose fluid nutrient medium, carry out shaken cultivation,Wherein, cultivation temperature is 25 DEG C, and the revolution of shaken cultivation is 200rpm, and incubation time is 48h, treats culture mediumAfter muddy, take out, put into 2-4 DEG C of Refrigerator store stand-by; By streptococcus lactis strain and Rhodopseudomonas palustris bacterial classificationAccess respectively in nutrient broth fluid nutrient medium and carry out shaken cultivation, wherein, cultivation temperature is 35 DEG C, vibrationThe revolution of cultivating is 195rpm, and incubation time is 35 hours, after culture medium muddiness, takes out, and puts into 2-4DEG C Refrigerator store is stand-by; By the 200000000/ml saccharomyces cerevisiae obtaining, 500,000,000/ml streptococcus lactis and 0.6 hundred million/mlRhodopseudomonas palustris mixes according to the weight proportion of 20:40:40, obtains composite bacteria;
Step 2: composite bacteria is carried out in culture medium to compound cultivation, obtain composite bacteria liquid, wherein, compoundThe condition of cultivating is to cultivate 120h under 30 DEG C of conditions, and the weight proportion of composite bacteria and culture medium is 5:95,Component and the weight proportion thereof of described culture medium preferably include: 5 parts, molasses, 1 part of peptone, 94 parts, water;
Step 3: described composite bacteria liquid is inoculated in the mixed system of fermentation waste water and molasses, under 32 DEG C of conditionsSealed fermenting 8 days, until pH is down to 3.5, finishes fermentation, wherein, and fermentation waste water, honey, compound bacteriaThe interpolation weight proportion of liquid is 100:5:12.
Embodiment 2
Utilize microorganism effectively to reduce a method for BOD in fermentation waste water, COD level, comprise following stepRapid:
Step 1: will in S. cervisiae bacterial classification access potato glucose fluid nutrient medium, carry out shaken cultivation,Wherein, cultivation temperature is 30 DEG C, and the revolution of shaken cultivation is 210rpm, and incubation time is 40h, treats culture mediumAfter muddy, take out, put into 2-4 DEG C of Refrigerator store stand-by; By streptococcus lactis strain and Rhodopseudomonas palustris bacterial classificationAccess respectively in nutrient broth fluid nutrient medium and carry out shaken cultivation, wherein, cultivation temperature is 32 DEG C, vibrationThe revolution of cultivating is 200rpm, and incubation time is 37 hours, after culture medium muddiness, takes out, and puts into 2-4DEG C Refrigerator store is stand-by; By the 200000000/ml saccharomyces cerevisiae obtaining, 500,000,000/ml streptococcus lactis and 0.6 hundred million/mlRhodopseudomonas palustris mixes according to the weight proportion of 25:40:35, obtains composite bacteria;
Step 2: composite bacteria is carried out in culture medium to compound cultivation, obtain composite bacteria liquid, wherein, compoundThe condition of cultivating is to cultivate 130h under 32 DEG C of conditions, and the weight proportion of composite bacteria and culture medium is 3:97,Component and the weight proportion thereof of described culture medium preferably include: 3 parts, molasses, 1 part of peptone, 96 parts, water;Step 3: described composite bacteria liquid is inoculated in the mixed system of fermentation waste water and molasses, airtight under 31 DEG C of conditionsFerment 10 days, until pH is down to 3.5, finish fermentation, wherein, fermentation waste water, honey, composite bacteria liquidInterpolation weight proportion is 100:5:10.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention alsoBe not restricted to specific embodiment described above. To those skilled in the art, any the present invention is carried outEquivalent modifications and substitute also all among category of the present invention. Therefore, do not departing from spirit of the present invention and modelEnclose lower done equalization conversion and amendment, all should contain within the scope of the invention.
Claims (9)
1. utilize microorganism effectively to reduce a method for BOD in fermentation waste water, COD level, it is characterized in that,Comprise the following steps:
Step 1: composite bacteria is carried out in culture medium to compound cultivation, obtain composite bacteria liquid, wherein, described inComposite bacteria is the combination of saccharomyces cerevisiae, streptococcus lactis, Rhodopseudomonas palustris;
Step 2: described composite bacteria liquid is inoculated in the mixed system of fermentation waste water and molasses, sealed fermenting,Until pH is down to 3.5, finish fermentation.
2. method according to claim 1, is characterized in that, in described step 1, and described saccharomyces cerevisiae(Saccharomycescerevisiae) bacterial classification that is ACCC20065 for deposit number; Described newborn hammerThe bacterial classification that bacterium (StreptococcusLactis) is ACCC10653 for deposit number; The red vacation in described marshThe bacterial classification that monad (Rhodopseudomonaspalustris) is ACCC10649 for deposit number.
3. method according to claim 1 and 2, is characterized in that, in described step 1, composite bacteria withThe weight proportion of culture medium is 1-10:90-99.
4. method according to claim 1, is characterized in that, also comprises the cultivation of bacterial classification, specifically comprises:
To in S. cervisiae bacterial classification access potato glucose fluid nutrient medium, carry out shaken cultivation, wherein, trainingFoster temperature is 20-35 DEG C, and the revolution of shaken cultivation is 180-220rpm, and incubation time is 20-48h, treats culture mediumAfter muddy, take out, put into 2-4 DEG C of Refrigerator store stand-by;
Streptococcus lactis strain and Rhodopseudomonas palustris bacterial classification are accessed respectively in nutrient broth fluid nutrient mediumRow shaken cultivation, wherein, cultivation temperature is 30-38 DEG C, the revolution of shaken cultivation is 180-220rpm, trainingThe foster time is 24-48 hour, after culture medium muddiness, takes out, and puts into 2-4 DEG C of Refrigerator store stand-by.
5. method according to claim 4, is characterized in that, through the incubation step of described bacterial classification, obtains200000000/ml saccharomyces cerevisiae, 500,000,000/ml streptococcus lactis and 0.6 hundred million/ml Rhodopseudomonas palustris, will be according toThe weight proportion of 20-25:40-44:31-40 mixes, and obtains composite bacteria.
6. method according to claim 1, is characterized in that, in described step 1, and the group of described culture mediumPoint and weight proportion comprise: molasses 1-5 part, peptone 0-1 part, water 94-99 part.
7. method according to claim 1, is characterized in that, in described step 1, described compound cultivationCondition is: under 20-35 DEG C of condition, cultivate 100-150h, obtain described composite bacteria liquid.
8. method according to claim 1, is characterized in that, in described step 2, fermentation waste water, honey,The interpolation weight proportion of composite bacteria liquid is 100-200:3-5:10-20.
9. according to the method described in claim 1 or 8, it is characterized in that, the condition of described sealed fermenting is: 20-35Sealed fermenting 5-10 days under DEG C condition, makes the pH of zymotic fluid be down to 3.5.
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| CN201511000061.9A CN105600943A (en) | 2015-12-28 | 2015-12-28 | Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater |
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| CN201511000061.9A CN105600943A (en) | 2015-12-28 | 2015-12-28 | Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962187A (en) * | 1996-10-25 | 1999-10-05 | Clarient International Ltd. | Radiation sensitive composition |
| CN1411872A (en) * | 2001-10-16 | 2003-04-23 | 广东省微生物研究所 | Microbial deodorant and its preparation method |
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
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- 2015-12-28 CN CN201511000061.9A patent/CN105600943A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962187A (en) * | 1996-10-25 | 1999-10-05 | Clarient International Ltd. | Radiation sensitive composition |
| CN1411872A (en) * | 2001-10-16 | 2003-04-23 | 广东省微生物研究所 | Microbial deodorant and its preparation method |
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
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