CN105594666A - Breeding method for fast increasing activity of antithrombase of leeches - Google Patents
Breeding method for fast increasing activity of antithrombase of leeches Download PDFInfo
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- CN105594666A CN105594666A CN201510989033.8A CN201510989033A CN105594666A CN 105594666 A CN105594666 A CN 105594666A CN 201510989033 A CN201510989033 A CN 201510989033A CN 105594666 A CN105594666 A CN 105594666A
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- 241000545744 Hirudinea Species 0.000 title claims abstract description 99
- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 230000001965 increasing effect Effects 0.000 title abstract 4
- 230000000694 effects Effects 0.000 claims abstract description 33
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims description 41
- 239000004019 antithrombin Substances 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 210000002966 serum Anatomy 0.000 claims description 14
- 230000002354 daily effect Effects 0.000 claims description 10
- 230000003592 biomimetic effect Effects 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 235000015192 vegetable juice Nutrition 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000012447 hatching Effects 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000003306 harvesting Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 17
- 238000012360 testing method Methods 0.000 description 15
- 239000012141 concentrate Substances 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 241000283690 Bos taurus Species 0.000 description 7
- 108090000190 Thrombin Proteins 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 229960004072 thrombin Drugs 0.000 description 7
- 230000037396 body weight Effects 0.000 description 4
- FYFFGSSZFBZTAH-UHFFFAOYSA-N methylaminomethanetriol Chemical compound CNC(O)(O)O FYFFGSSZFBZTAH-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
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- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000037406 food intake Effects 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- MCHZKGNHFPNZDP-UHFFFAOYSA-N 2-aminoethane-1,1,1-triol;hydrochloride Chemical compound Cl.NCC(O)(O)O MCHZKGNHFPNZDP-UHFFFAOYSA-N 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 244000169997 Schnittsellerie Species 0.000 description 2
- 235000013559 Schnittsellerie Nutrition 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 235000020384 spinach juice Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 101100042371 Caenorhabditis elegans set-3 gene Proteins 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 238000006424 Flood reaction Methods 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000009863 impact test Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- 230000004083 survival effect Effects 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
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- A01K67/033—
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a breeding method for fast increasing the activity of antithrombase of leeches. The breeding method comprises the following steps of 1 young leech collection, 2 weight-increasing breeding, 3 temperature control and feed deprivation and 4 harvesting. The breeding method for fast increasing the activity of the antithrombase of the leeches is short in breeding period, the activity of the antithrombase with unit mass of the leeches can be fast improved in the short term, the activity of the antithrombase with unit mass of the leeches can be improved by 35.28% to 116.61% within one month, the medicinal value of the leeches is effectively improved, the temporary keeping time is shortened, production management cost is reduced, breeding risks are reduced, and therefore the economic benefits of breeding can be increased.
Description
Technical field
The cultural method that the present invention relates to a kind of quick increase leech antithrombin activity, specifically belongs to hirudiniculture technical field.
Background technology
Hirudiniculture generally adopts kind of the alternate land for growing field crops of leech floods and droughts furrow formula cultivation and cement pit to concentrate two kinds of patterns of cultivation. Land for growing field crops cultivationRefer to concentrate after natural spawning hatching and grow seedlings point pool cultivated or parent and seedling with pond mixed breed formula, operating procedure is simple, be easy to manage,But be subject to Effect of Natural Disaster larger, wild environment leech natural enemy is many, and disease control difficulty is larger, and growing-seedling period is long and density is little, largeField cultivation is directly fished for for 1 to 2 year afterwards, and this kind of pattern individual weight gain rate is very little, mainly produces economic valency by the multiplication of leech quantityValue. After cement pit is concentrated cultivation to refer to concentrate annual 4, the little individual leech of buying in May to raise to 7, stop eating 2 months August, adoptReceive and sell, this kind of pattern do not have the increase of leech quantity, is mainly to produce economic benefit by the multiplication of whose body weight. Above two kindsAll there is the problem that the culture-cycle is long, production cost is high in mode, and cement pit cultivation is simply stopped eating processing to improving leech unitThe efficiency of mass reactance thrombin activity is lower and body weight loss is larger. Therefore provide a kind of culture-cycle short, can be efficiently, increase fastThe cultural method that adds leech antithrombin activity seems particularly necessary.
Summary of the invention
For solving the deficiencies in the prior art, the object of the present invention is to provide a kind of cultivation of quick increase leech antithrombin activityMethod, can effectively improve leech antithrombin activity fast.
In order to realize above-mentioned target, the present invention adopts following technical scheme:
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers; (2) weightening finishCultivation; (3) temperature control is stopped eating; (4) results.
Particularly, aforementioned cultural method comprises the following steps: (1) seedling gathers: collect the leech seedling after hatching, concentrate and educateSeedling divides seedling processing after 20 days; (2) weightening finish cultivation: after dividing seedling, leech seedling is placed in daily raising at 22 DEG C～34 DEG C temperatureFeed 2 months, fast weightening finish; (3) temperature control is stopped eating: raising temperature is controlled to 30 DEG C, and constant temperature is stopped eating 1 month; (4) results.
In aforementioned cultural method, in the breeding process of step (2) and (3), clean and change water, every day uniform tempClean water once cleaned and changed, choose dead leech simultaneously.
In aforementioned cultural method, in step (2), cultivation density is 60～80 tails/L.
In aforementioned cultural method, daily feeding as the bait of throwing something and feeding taking Biomimetic membranes as medium one time for every 4 days in step (2).
In aforementioned cultural method, described bait can be cow's serum, the former blood of ox, pig serum or the former blood of pig, can be also volume ratioBe the vegetable juice of 1～5:6～10 (being preferably 2: 8) and the mixture of cow's serum/pig serum; Described vegetable juice can be celeryJuice, spinach juice or smallage juice.
Further, in aforementioned cultural method, before Feeding time is preferably chosen in 12:00 in the morning, be conducive to improve anticoagulationEnzyme content.
When daily feeding, leech is cleaned up before throwing something and feeding, carries out feeding after being filtered dry water, eating time be controlled at 30min～90min。
In leech feed process, choose at any time excessive individuality, to control its single food-intake, prevent swollen dead.
Throw something and feed after finishing leech is separated with the container of throwing something and feeding, leech cleans up to put back in feeder and deposits, the cleaning container of throwing something and feeding,Dry while getting inferior throwing something and feeding ready and reuse.
One, the impact test of temperature on leech growth
1, test objective
Add up by designing different thermograde cultivation leech, record the survival rate that leech grows under various temperature environments, pointAnalyse and determine leech optimum growth temperature.
2, content of the test
2.1 experimental design
(1) 18 DEG C, 22 DEG C, 26 DEG C, 30 DEG C, 34 DEG C, 38 DEG C and seven grades of natural environment temperature, each temperature etc. are set3 repetitions of level, each repetition 50 healthy leech seedlings in tail 1g left and right; (2) matrix: water; (3) humidity: wet under natural conditionsDegree; (4) density: 62.5 tails/L; (5) test period: 60 days.
2.2 test material
(1) test chamber: 2000mL large beaker; (2) substrates quantity: 800mL; (3) the anti-net of escaping: 40 order nylon wires; (4)Bait: fresh bovine serum
2.3 test daily managements
(1) clean and change water: every day, clear water cleaned one time; (2) mode of throwing something and feeding: Biomimetic membranes suction type; (3) frequency of throwing something and feeding: 4It once; (4) duration of throwing something and feeding: 30min/ time; (5) necrology: once dead leech quantity and weight of statistics every day.
3, result of the test
From dead quantity and the weight statistics of leech, growing environment temperature is in the time of 20 DEG C～30 DEG C, and leech rate of body weight gainHeight, especially taking under 30 DEG C of conditions as best.
Two, the temperature control impact on leech antithrombin activity of stopping eating
In leech body, antithrombase mainly concentrates on the head of leech, the impact of the variation of leech body weight on overall antithrombin activityRelatively little, therefore, the present invention mainly embodies the antithrombin activity of leech by the antithrombase content of leech unit mass,And the antithrombase content that adopts following method to measure leech unit mass is studied the shadow that temperature control is stopped eating to leech antithrombin activityRing.
1, the preparation of solution
(1) preparation of 0.2mol/L trihydroxy methyl aminomethane solution
Get trihydroxy methyl aminomethane (analyzing pure) 2.4228g, add water to 100mL, be 0.2mol/L trihydroxy methylAminomethane solution.
(2) preparation of 0.1mol/L hydrochloric acid solution
Get concentrated hydrochloric acid 9mL, add water to 1000mL, be 0.1mol/L hydrochloric acid solution.
(3) preparation of trihydroxy methyl aminomethane hydrochloride buffer
Get 0.2mol/L trihydroxy methyl aminomethane solution 25mL and 0.1mol/L hydrochloric acid solution 40mL adds water to 100mL, fillDivide and mix, regulate pH to 7.4, to obtain final product.
The preparation of (4) 0.5% BFGs (with coagulum note) solution
Get in BFG (containing coagulum 59%) 0.085g to 10mL volumetric flask, with three hydroxyls that are heated in advance 37 DEG CYlmethyl aminomethane hydrochloride buffer is diluted to scale, and that 37 DEG C of water-bath jogs slowly make is molten, and (putting into the water-bath time should not exceed 10Minute).
(5) preparation of thrombin solution
Getting content is that 560U/ props up 1 of fibrin ferment, gets its entire contents to 10mL volumetric flask, with normal saline dilution extremelyScale, shakes up gently and is 56U/mL, is contained in glass tube.
(6) preparation of need testing solution
Get the about 1g of Leech (No. 3 sieves), accurately weighed, precision adds 0.9% sodium chloride solution 5mL, fully stirs, and soaksCarry 30 minutes, and jolting constantly, 3500r/min is centrifugal, centrifugal 15 minutes, gets supernatant.
2, assay operating procedure
(1) precision measures supernatant 100 μ L, is placed in (10mm × 75mm) test tube;
(2) precision adds the trihydroxy methyl aminomethane hydrochloride buffer 200 μ L containing 0.5% BFG, shakes up;
(3) test tube being placed in to water-bath (37 ± 0.5 DEG C) (put water-bath with thermometer and observe measurement temperature) slowly dripsEvery 1mL is containing the thrombin solution (5 μ L per minute, dropping limit, limit shakes up gently) of 56 U of unit, most while dripping thrombin solutionAmount is not taken out test tube, so that it remains in 37 ± 0.5 DEG C of temperature. When approaching while solidifying, 5 μ L gradation drip, drip 1 at every turn～2 μ L, dropping limit, limit jog, whether close observation it solidify (can put test tube in printing opacity place), and available syringe needle point is gently chosen, and seesWhether there is filiform to produce, solidify if having filiform or floccule to produce to be judged to. Each sample is put the time of detecting in water-bathMust not more than 30 minutes.
(4) record consumes the volume of thrombin solution, calculates according to the following formula its content.
In formula, U is that every 1g is containing antithrombin activity unit, U/g; C1For the concentration of thrombin solution, U/mL; C2For test sampleThe concentration of solution, g/mL; V1For consuming the volume of thrombin solution, μ L; V2For the addition of need testing solution, μ L; NeutralizationThe amount of the fibrin ferment of a unit is an antithrombin activity unit.
Measure respectively the weightening finish cultivation stage finish after and stop eating 1 month after the antithrombase content of leech unit mass, result asShown in table 1.
The antithrombase assay result of table 1 leech unit mass
As shown in Table 1, temperature control is stopped eating and is raised after 1 month, and the antithrombase content of leech unit mass has had significant raising.When the phase temperature of wherein stopping eating is 30 DEG C, the antithrombase content raising amount maximum of leech unit mass, therefore, the present invention selectsThe stop eating temperature in stage of 30 DEG C of conducts.
In cultural method of the present invention, also can adopt volume ratio is that the vegetable juice of 1～5:6～10 and the mixture of cow's serum/pig serum replaceIn generation,, conventional bait cultivated. Cultivate according to the method described above and test, measure temperature control stop eating 1 month after leech unit massAntithrombase content. Result is as shown in table 2.
The antithrombase assay result of table 2 leech unit mass
As shown in Table 2, in cultural method of the present invention, adopting volume ratio is the vegetable juice of 1～5:6～10 and cow's serum/pig serumThe conventional bait of mixture replacing cultivates, and the antithrombase content of leech unit mass improves more remarkable.
Usefulness of the present invention is: the present invention increases the cultural method of leech antithrombin activity fast, and the culture-cycle is short,Can improve fast in a short time the antithrombin activity of leech unit mass, the antithrombin activity of leech unit mass in monthCan improve 35.28%～116.61%, not only effectively improve leech medical value, and shorten temporarily and support the time, reduce production managementCost, reduces cultivation risk, thereby can improve fanning economics. If adopt isolation cultivation, also help disease control,Can reduce cross infection of disease, significantly improve fanning economics.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further introduced.
Embodiment 1
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers: collect hatchingAfter leech seedling, concentrate after growing seedlings 20 days and divide seedling processing; (2) weightening finish cultivation: leech seedling is placed in to 22 DEG C of temperatureLower daily feeding 2 months, fast weightening finish; Cultivation density is 60 tails/L, and the bait of throwing something and feeding taking Biomimetic membranes as medium for every 4 days is (newFresh cow's serum), before Feeding time is chosen in 12:00 in the morning, leech is cleaned up before throwing something and feeding, carry out feeding after being filtered dry water,Eating time is controlled at 30min; In leech feed process, choose at any time excessive individuality, to control its single food-intake, preventSwollen dead; (3) temperature control is stopped eating: raising temperature is controlled to 30 DEG C, and constant temperature is stopped eating 1 month; (4) results. In addition, in stepAnd in the breeding process of (3), also need to clean to change water (2), every day once cleaned and changed with the clean water of uniform temp,Choose dead leech simultaneously.
Embodiment 2
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers: collect hatchingAfter leech seedling, concentrate after growing seedlings 20 days and divide seedling processing; (2) weightening finish cultivation: leech seedling is placed in to 34 DEG C of temperatureLower daily feeding 2 months, fast weightening finish; Cultivation density is 80 tails/L, and the bait of throwing something and feeding taking Biomimetic membranes as medium for every 4 days is (newFresh porcine blood is clear), before Feeding time is chosen in 12:00 in the morning, leech is cleaned up before throwing something and feeding, carry out feeding after being filtered dry water,Eating time is controlled at 90min; (3) temperature control is stopped eating: raising temperature is controlled to 30 DEG C, and constant temperature is stopped eating 1 month; (4) receiveObtain. In addition, in the breeding process of step (2) and (3), also need to clean and change water, use the clean of uniform temp every dayWater purification is once cleaned and changed, chooses dead leech simultaneously.
Embodiment 3
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers: collect hatchingAfter leech seedling, concentrate after growing seedlings 20 days and divide seedling processing; (2) weightening finish cultivation: the leech seedling of 1g left and right is placed inDaily feeding 2 months at 30 DEG C of temperature, fast weightening finish; Cultivation density is 75 tails/L, within every 4 days, throws something and feeds one taking Biomimetic membranes as mediumInferior bait (Celery Juice that volume ratio is 2:8 and the mixture of cow's serum), before Feeding time is chosen in 12:00 in the morning, throws something and feedsLeech is cleaned up before, carry out feeding after being filtered dry water, eating time is controlled at 60min; In leech feed process, choose at any timeGo out excessive individuality, to control its single food-intake, prevent swollen dead; Throw something and feed finish after leech is separated with the container of throwing something and feeding, leechClean up to put back in feeder and deposit, after the cleaning container of throwing something and feeding, dry for subsequent use; (3) temperature control is stopped eating: raising temperature is controlled at30 DEG C, constant temperature is stopped eating 1 month; (4) results. In addition, in the breeding process of step (2) and (3), also need to carry out clearlyWash water, use every day the clean water of uniform temp once cleaned and changed, choose dead leech simultaneously.
Embodiment 4
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers: collect hatchingAfter leech seedling, concentrate after growing seedlings 20 days and divide seedling processing; (2) weightening finish cultivation: the leech seedling of 1g left and right is placed inDaily feeding 2 months at 26 DEG C of temperature, fast weightening finish; Cultivation density is 65 tails/L, within every 4 days, throws something and feeds one taking Biomimetic membranes as mediumInferior bait (spinach juice that volume ratio is 1:9 and the mixture of cow's serum), before Feeding time is chosen in 12:00 in the morning, throws something and feedsLeech is cleaned up before, carry out feeding after being filtered dry water, eating time is controlled at 75min; In leech feed process, choose at any timeGo out excessive individuality, prevent swollen dead; Throw something and feed finish after leech is separated with the container of throwing something and feeding, leech cleans up puts back in feederDeposit, after the cleaning container of throwing something and feeding, dry for subsequent use; (3) temperature control is stopped eating: raising temperature is controlled to 30 DEG C, and constant temperature is stopped eating 1Month; (4) results. In addition, in the breeding process of step (2) and (3), also need to clean and change water, every day phaseSynthermal clean water is once cleaned and changed, chooses dead leech simultaneously.
Embodiment 5
A cultural method for quick increase leech antithrombin activity, comprises the following steps: (1) seedling gathers: collect hatchingAfter leech seedling, concentrate after growing seedlings 20 days and divide seedling processing; (2) weightening finish cultivation: leech seedling is placed in to natural environment temperatureLower daily the feeding 2 months of degree, weightening finish fast; Cultivation density is 70 tails/L, the bait of throwing something and feeding taking Biomimetic membranes as medium one time for every 4 days(the smallage juice that volume ratio is 4:10 and the mixture of pig serum); (3) temperature control is stopped eating: raising temperature is controlled to 30 DEG C,Constant temperature is stopped eating 1 month; (4) results. In addition, in the breeding process of step (2) and (3), also need to clean and change water,Be that every day is once cleaned and changed with the clean water of uniform temp, choose dead leech simultaneously.
Claims (10)
1. a cultural method that increases fast leech antithrombin activity, is characterized in that: comprise the following steps: (1) seedling gathers;(2) weightening finish cultivation; (3) temperature control is stopped eating; (4) results.
2. the cultural method of quick increase leech antithrombin activity according to claim 1, is characterized in that: comprise following stepRapid: (1) seedling gathers: to collect the leech seedling after hatching, after concentrated growing seedlings 20 days, divide seedling processing; (2) weightening finish cultivation:After dividing seedling, leech seedling is placed in daily feeding 2 months at 22 DEG C～34 DEG C temperature, fast weightening finish; (3) temperature control is stopped eating: will raiseFoster temperature is controlled at 30 DEG C, and constant temperature is stopped eating 1 month; (4) results.
3. the cultural method of quick increase leech antithrombin activity according to claim 1 and 2, is characterized in that: in step(2) and in the breeding process of (3) clean and change water, use every day the clean water of uniform temp once cleaned and changed, simultaneouslyChoose dead leech.
4. the cultural method of quick increase leech antithrombin activity according to claim 1 and 2, is characterized in that: step (2)Middle cultivation density is 60～80 tails/L.
5. the cultural method of quick increase leech antithrombin activity according to claim 2, is characterized in that: step (2)In daily feeding as the bait of throwing something and feeding taking Biomimetic membranes as medium one time for every 4 days.
6. the cultural method of quick increase leech antithrombin activity according to claim 5, is characterized in that: described bait isCow's serum, the former blood of ox, pig serum or the former blood of pig, or volume ratio be 1～5:6～10 vegetable juice and cow's serum/pig serumMixture.
7. the cultural method of quick increase leech antithrombin activity according to claim 5, is characterized in that: Feeding time choosingSelected before morning 12:00.
8. the cultural method of quick increase leech antithrombin activity according to claim 5, is characterized in that: when daily feeding,Leech is cleaned up before throwing something and feeding, carry out feeding after being filtered dry water, eating time is controlled at 30min～90min.
9. the cultural method of quick increase leech antithrombin activity according to claim 5, is characterized in that: leech was taken foodIn journey, choose at any time excessive individuality, prevent swollen dead.
10. the cultural method of quick increase leech antithrombin activity according to claim 5, is characterized in that: the end of throwing something and feedingAfter leech is separated with the container of throwing something and feeding, leech cleans up to put back in feeder and deposits, and dries for subsequent use after the cleaning container of throwing something and feeding.
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| CN109937927A (en) * | 2017-12-21 | 2019-06-28 | 南京农业大学 | A kind of method of quick raising leech antithrombase content |
| CN112493186A (en) * | 2020-11-13 | 2021-03-16 | 重庆市药物种植研究所 | Culture method for rapidly increasing anticoagulant enzyme activity of hirudo nipponica |
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
| CN115316319A (en) * | 2022-08-29 | 2022-11-11 | 重庆多普泰制药股份有限公司 | Cultivation method for improving activity of antithrombin of leeches |
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| 孙国梅等: "《药用水蛭养殖与人工繁育》", 31 January 2013, 科学技术文献出版社 * |
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| CN109937927A (en) * | 2017-12-21 | 2019-06-28 | 南京农业大学 | A kind of method of quick raising leech antithrombase content |
| CN112493186A (en) * | 2020-11-13 | 2021-03-16 | 重庆市药物种植研究所 | Culture method for rapidly increasing anticoagulant enzyme activity of hirudo nipponica |
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
| CN115316319A (en) * | 2022-08-29 | 2022-11-11 | 重庆多普泰制药股份有限公司 | Cultivation method for improving activity of antithrombin of leeches |
| CN115316319B (en) * | 2022-08-29 | 2023-07-07 | 重庆多普泰制药股份有限公司 | Breeding method for improving antithrombin activity of leeches |
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