CN105541982A - Zein and pigment extraction method - Google Patents
Zein and pigment extraction method Download PDFInfo
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- CN105541982A CN105541982A CN201510758780.0A CN201510758780A CN105541982A CN 105541982 A CN105541982 A CN 105541982A CN 201510758780 A CN201510758780 A CN 201510758780A CN 105541982 A CN105541982 A CN 105541982A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/425—Zeins
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- Life Sciences & Earth Sciences (AREA)
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- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a zein and pigment extraction method, and belongs to the technical field of agricultural product processing. Corn protein powder adopted as a raw material is extracted with a 95% ethanol solution at a temperature of 50DEG C according to a solid-to-liquid ratio of 1:15 for 5h to realize a best effect, the purity of obtained protein extract reaches 84.3%, and the extraction rate is 89.6%. The yield of an extracted maize yellow pigment is 1.125%, and the extraction rate of beta-carotene in the corn protein powder is 0.5458mg/g.
Description
Technical field
The present invention relates to the extracting method of a kind of zein and pigment, belong to deep-processing technical field of agricultural products.Zein is applicable to all kinds of capsule-shaped food and makes, and pigment can as functional food ingredient.
Background technology
Corn is one of world's Three major grain crops, and corn is important conventional food.According to statistics, within 2013, Chinese maize output reaches 2.11 hundred million tons, and amplification is 5.9%, and output is the second in the world, and be volume increase in continuous 5th year, 2014 annual production are up to 2.15 hundred million tons.Within 2011, Chinese maize import volume is 1,750,000 tons, import corn 5,200,000 tons in 2012.Though maize kernel starch-containing more than 70%, its protein content is higher than rice, and lipid content is higher than flour, rice and millet, and heat content is higher than flour, rice and Chinese sorghum.In Semen Maydis, maize yellow cellulose content is especially high, also containing abundant natural yellow pigment in Zein powder, with the development of society, natural pigment is more and more subject to the favor of people, corn yellow OB have anti-oxidant in body, contribute to the several functions such as absorption of nutrient ingredients, antitumous effect, the senile eye illness of prevention, corn pigment is approved as food dye by the states such as the U.S., Britain, Japan, France, Australia, the European Economic Community and area and uses, and corn yellow OB has been classified as edible natural pigment by China GB2760 standard; Zein is also known as prolamine, and have the characteristics such as protection against the tide, oxygen barrier, guarantor's perfume, oil resistance, having certain bacteriostatic action, is desirable crude antistaling agent.So utilize the albumen in Zein powder and pigment resource to have very large market outlook.
Summary of the invention
For solving the problem, the object of the present invention is to provide the extracting method of a kind of zein and pigment.
The invention provides the extracting method of a kind of zein and pigment, it is characterized in that extraction process flow process is: (1) weighs Zein powder; (2) at solid-liquid ratio 1: 15,95% ethanolic soln, temperature 60 C, extracts under time 5h condition; (4) centrifugation, selection rotating speed is 2000rpm, time 12min, and obtain throw out and alcohol solubleness extraction liquid, can be used as fodder additives after throw out drying, alcohol solubleness extraction liquid includes prolamine and pigment; (5) by alcohol solubleness extraction liquid vacuum concentration to original volume half, the pure water then adding 4 times of volumes dilutes; (6) the extraction liquid adjust ph after dilution to 6.0, then be cooled to-15 DEG C of temperature, filter and take off a layer throw out, with distillation washing 3 times, record into neutrality with pH test paper, washing thing is placed in 45 DEG C of baking ovens, obtain zein extract through 8h oven dry to constant weight; (7) filtrate concentrates through rotavapor under vacuum, until do not distilling out ethanol, finally obtains corn yellow OB.
Extraction and the centrifugally operated of Zein powder do extraction liquid with ethanolic soln, select solid-liquid ratio, temperature, extraction time to be factor, optimization of orthogonal test extracting parameter; Centrifugation rotating speed elects 2000rpm time 12min as.The alcohol solubleness extraction liquid obtained includes prolamine and pigment is maximum.Parameters optimization is 95% ethanolic soln extraction, at solid-to-liquid ratio 1: 15, temperature 50 C, extraction Zein powder 5h;
Corn yellow OB drying means is that filtrate concentrates through rotavapor under vacuum, and consider that corn yellow OB is better at 50 DEG C of stability inferiors, the warm air drying at 50 DEG C is selected in drying operation.
Compared with prior art, the present invention has following beneficial effect:
(1) the present invention optimizes processing parameter in extraction, make zein and corn yellow OB extraction yield higher.
(2) the present invention has the originality of lixiviate that zein and corn yellow OB combined, and has good application value.
(3) the adjustable people's physiological function of product of the present invention, supplements the nutrients, provides functional food raw material, promotes the deep processing of corn.
Embodiment
Below in conjunction with specific embodiment, the present invention is further detailed.
embodiment 1:
Zein powder 20.000g, at solid-liquid ratio 1: 15,95% ethanolic soln, temperature 60 C, extracts under time 5h condition.Centrifugation rotating speed elects 2000rpm time 12min as.The alcohol solubleness extraction liquid obtained includes prolamine and pigment.By alcohol solubleness extraction liquid vacuum concentration to original volume half, the pure water then adding 4 times of volumes dilutes.The extraction liquid adjust ph after dilution to 6.0, then be cooled to-15 DEG C of freezing temps.Get lower sediment, with distillation washing 3 times, record into neutrality with pH test paper.Washing thing is placed in 45 DEG C of baking ovens, obtains zein extract through 8h oven dry to constant weight.Filtrate concentrates through rotavapor under vacuum, and consider that corn yellow OB is better at 50 DEG C of stability inferiors, the warm air drying at 50 DEG C is selected in drying operation.Obtain zein extract 4.70g, the content recording protein through Kjeldahl determination is: 84.3%.Empirically recording raw material containing protein 55.3%, is 40% calculating in ratio contained by the prolamine in corn, extraction using alcohol prolamine extraction yield 89.6%.Again by filtrate-corn yellow OB liquid distillation and concentration, until do not distilling out ethanol, the weight of the corn yellow OB claiming last is being 0.225g.Extract goes out rate 1.125%, in β-carotene extraction yield 0.5458mg/g in Zein powder.
embodiment 2:
Zein powder 60.000g, at solid-liquid ratio 1: 15,95% ethanolic soln, temperature 60 C, extracts under time 5h condition.Centrifugation rotating speed elects 2000rpm time 12min as.The alcohol solubleness extraction liquid obtained includes prolamine and pigment.By alcohol solubleness extraction liquid vacuum concentration to original volume half, the pure water then adding 4 times of volumes dilutes.The extraction liquid adjust ph after dilution to 6.0, then be cooled to-15 DEG C of freezing temps.Get lower sediment, with distillation washing 3 times, record into neutrality with pH test paper.Washing thing is placed in 45 DEG C of baking ovens, obtains zein extract through 8h oven dry to constant weight.Filtrate concentrates through rotavapor under vacuum, and consider that corn yellow OB is better at 50 DEG C of stability inferiors, the warm air drying at 50 DEG C is selected in drying operation.Obtain zein extract 14.10g, the content recording protein through Kjeldahl determination is: 84.3%.Empirically recording raw material containing protein 55.3%, is 40% calculating in ratio contained by the prolamine in corn, extraction using alcohol prolamine extraction yield 89.6%.Again by filtrate-corn yellow OB liquid distillation and concentration, until do not distilling out ethanol, the weight of the corn yellow OB claiming last is being 0.675g, and extract goes out rate 1.125%, β-carotene pigment extraction yield 1.637mg/g in Zein powder.
embodiment 3:
Zein powder 50.000g, at solid-liquid ratio 1: 15,95% ethanolic soln, temperature 60 C, extracts under time 5h condition.Centrifugation rotating speed elects 2000rpm time 12min as.The alcohol solubleness extraction liquid obtained includes prolamine and pigment.By alcohol solubleness extraction liquid vacuum concentration to original volume half, the pure water then adding 4 times of volumes dilutes.The extraction liquid adjust ph after dilution to 6.0, then be cooled to-15 DEG C of freezing temps.Get lower sediment, with distillation washing 3 times, record into neutrality with pH test paper.Washing thing is placed in 45 DEG C of baking ovens, obtains zein extract through 8h oven dry to constant weight.Filtrate concentrates through rotavapor under vacuum, and consider that corn yellow OB is better at 50 DEG C of stability inferiors, the warm air drying at 50 DEG C is selected in drying operation.Obtain zein extract 11.75g, the content recording protein through Kjeldahl determination is: 84.3%.Empirically recording raw material containing protein 55.3%, is 40% calculating in ratio contained by the prolamine in corn, extraction using alcohol prolamine extraction yield 89.6%.Again by filtrate-corn yellow OB liquid distillation and concentration, until do not distilling out ethanol, the weight of the corn yellow OB claiming last is being 0.5625g, and extract goes out rate 1.125%, β-carotene pigment extraction yield 1.3645mg/g in Zein powder.
Above embodiment is only the preferred embodiment of this creation, and not in order to limit this creation, any amendment made within all spirit in this creation and principle, equivalent replacement, improvement etc., within the protection domain that all should be included in this creation.
Claims (4)
1. an extracting method for zein and pigment, is characterized in that its technical process is: (1) weighs Zein powder; (2) at solid-liquid ratio 1: 15,95% ethanolic soln, temperature 60 C, extracts under time 5h condition; (4) centrifugation, selection rotating speed is 2000rpm, time 12min, and obtain throw out and alcohol solubleness extraction liquid, can be used as fodder additives after throw out drying, alcohol solubleness extraction liquid includes prolamine and pigment; (5) by alcohol solubleness extraction liquid vacuum concentration to original volume half, the pure water then adding 4 times of volumes dilutes; (6) the extraction liquid adjust ph after dilution to 6.0, then be cooled to-15 DEG C of temperature, filter and take off a layer throw out, with distillation washing 3 times, record into neutrality with pH test paper, washing thing is placed in 45 DEG C of baking ovens, obtain zein extract through 8h oven dry to constant weight; (7) filtrate concentrates through rotavapor under vacuum, until do not distilling out ethanol, finally obtains corn yellow OB.
2. the extracting method of zein according to claim 1 and pigment, is characterized in that: it take Zein powder as raw material, with 95% ethanolic soln extraction, at solid-to-liquid ratio 1: 15, temperature 50 C, extraction Zein powder 5h.
3. the extracting method of zein according to claim 1 and pigment, it is characterized in that: described zein extracting method, extraction and the centrifugally operated of Zein powder do extraction liquid with ethanolic soln, solid-liquid ratio, temperature, extraction time is selected to be factor, optimization of orthogonal test extracting parameter; Centrifugation rotating speed elects 2000rpm time 12min as, and the corn alcohol solubleness extraction liquid of acquisition includes prolamine and pigment is maximum.
4. the extracting method of zein according to claim 1 and pigment, is characterized in that: described corn yellow OB drying means is that filtrate concentrates through rotavapor under vacuum, and corn yellow OB is warm air drying at 50 DEG C of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510758780.0A CN105541982A (en) | 2015-11-10 | 2015-11-10 | Zein and pigment extraction method |
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| CN201510758780.0A CN105541982A (en) | 2015-11-10 | 2015-11-10 | Zein and pigment extraction method |
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| CN105541982A true CN105541982A (en) | 2016-05-04 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106349742A (en) * | 2016-08-30 | 2017-01-25 | 杭州上拓环境科技股份有限公司 | Membrane treatment system for extracting corn peptide and corn yellow pigment and treatment technology |
| CN106867652A (en) * | 2016-12-29 | 2017-06-20 | 齐鲁工业大学 | A kind of continuous method for preparing millet cavings crude oil and alcohol soluble protein |
| WO2019060179A1 (en) * | 2017-09-21 | 2019-03-28 | Cargill, Incorporated | Corn protein retention during extraction |
| CN114601175A (en) * | 2021-12-28 | 2022-06-10 | 六安益普罗科技有限公司 | Preparation method of enteric slow-release high-activity glutathione nanospheres |
| US11375736B2 (en) | 2016-03-24 | 2022-07-05 | Cargill, Incorporated | Corn protein product having decreased free sulfite levels and method for manufacturing same |
| US11980217B2 (en) | 2017-08-02 | 2024-05-14 | Cargill, Incorporated | Extruded corn protein material |
| US11985990B2 (en) | 2016-09-23 | 2024-05-21 | Cargill, Incorporated | Corn protein retention during extraction |
| US12054515B2 (en) | 2015-03-24 | 2024-08-06 | Cargill, Incorporated | Corn protein isolate and methods of manufacturing same |
-
2015
- 2015-11-10 CN CN201510758780.0A patent/CN105541982A/en not_active Withdrawn
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12054515B2 (en) | 2015-03-24 | 2024-08-06 | Cargill, Incorporated | Corn protein isolate and methods of manufacturing same |
| US11375736B2 (en) | 2016-03-24 | 2022-07-05 | Cargill, Incorporated | Corn protein product having decreased free sulfite levels and method for manufacturing same |
| CN106349742B (en) * | 2016-08-30 | 2018-08-07 | 杭州上拓环境科技股份有限公司 | A kind of film processing system and treatment process of extraction Semen Maydis polypeptide and corn yellow OB |
| CN106349742A (en) * | 2016-08-30 | 2017-01-25 | 杭州上拓环境科技股份有限公司 | Membrane treatment system for extracting corn peptide and corn yellow pigment and treatment technology |
| US11985990B2 (en) | 2016-09-23 | 2024-05-21 | Cargill, Incorporated | Corn protein retention during extraction |
| CN106867652A (en) * | 2016-12-29 | 2017-06-20 | 齐鲁工业大学 | A kind of continuous method for preparing millet cavings crude oil and alcohol soluble protein |
| US11980217B2 (en) | 2017-08-02 | 2024-05-14 | Cargill, Incorporated | Extruded corn protein material |
| CN111132557B (en) * | 2017-09-21 | 2023-06-30 | 嘉吉公司 | Corn protein retention during extraction |
| US11667670B2 (en) | 2017-09-21 | 2023-06-06 | Cargill, Incorporated | Corn protein retention during extraction |
| EP3684191A4 (en) * | 2017-09-21 | 2021-07-07 | Cargill, Incorporated | Corn protein retention during extraction |
| CN111132557A (en) * | 2017-09-21 | 2020-05-08 | 嘉吉公司 | Zein retention during extraction |
| WO2019060179A1 (en) * | 2017-09-21 | 2019-03-28 | Cargill, Incorporated | Corn protein retention during extraction |
| CN114601175A (en) * | 2021-12-28 | 2022-06-10 | 六安益普罗科技有限公司 | Preparation method of enteric slow-release high-activity glutathione nanospheres |
| CN114601175B (en) * | 2021-12-28 | 2023-09-05 | 六安益普罗科技有限公司 | Preparation method of enteric sustained-release high-activity glutathione nano-microsphere |
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Application publication date: 20160504 |