CN105481215A - Method for recovering crude oil in oil-containing hazardous wastes by utilizing microbial technology - Google Patents
Method for recovering crude oil in oil-containing hazardous wastes by utilizing microbial technology Download PDFInfo
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- 239000010779 crude oil Substances 0.000 title claims abstract description 80
- 239000003921 oil Substances 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 37
- 230000000813 microbial effect Effects 0.000 title claims abstract description 15
- 238000005516 engineering process Methods 0.000 title abstract description 7
- 239000002920 hazardous waste Substances 0.000 title abstract description 7
- 238000011084 recovery Methods 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 239000002689 soil Substances 0.000 claims abstract description 24
- 238000005273 aeration Methods 0.000 claims abstract description 23
- 244000005700 microbiome Species 0.000 claims abstract description 10
- 239000002910 solid waste Substances 0.000 claims abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 4
- 230000012010 growth Effects 0.000 claims abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 28
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 18
- 239000011707 mineral Substances 0.000 claims description 18
- 235000016709 nutrition Nutrition 0.000 claims description 18
- 230000035764 nutrition Effects 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 14
- 230000000050 nutritive effect Effects 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 238000004064 recycling Methods 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000000839 emulsion Substances 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- 239000004575 stone Substances 0.000 claims description 7
- 239000008399 tap water Substances 0.000 claims description 7
- 235000020679 tap water Nutrition 0.000 claims description 7
- -1 wooden unit Substances 0.000 claims description 7
- 238000012136 culture method Methods 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000013543 active substance Substances 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 230000005526 G1 to G0 transition Effects 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 230000010261 cell growth Effects 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 6
- 239000010802 sludge Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000013049 sediment Substances 0.000 abstract 4
- 241000894006 Bacteria Species 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 229910017053 inorganic salt Inorganic materials 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000007670 refining Methods 0.000 abstract 1
- 239000002002 slurry Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000007667 floating Methods 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 150000001454 anthracenes Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001555 benzenes Chemical class 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001698 pyrogenic effect Effects 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a technology for performing resourceful treatment on ground oil soil/ oil sludge/ oil sediment by utilizing efficient microorganisms. A screened microbial compound bacterium separates and extracts crude oil in oil-containing hazardous wastes for substantially reducing crude oil content in oil soil/oil sludge/oil sediment. After being sieved for removing fragments, ground oil soil/ oil sludge/ oil sediment coming from oil extraction and oil refining industries and an inorganic salt culture solution are poured into a biological slurry reactor according to a certain solid-liquid ratio, the mixture is inoculated with an efficient microbial flora, and an inocula is supplemented according to the flora growth case during running. After running is performed for 3-9 days, crude oil is separated from the substrate and floats upwards to the liquid surface under the aeration (air floatation) effect, and thus recovery is realized. The crude oil recovery rate is 60-90%, and the solid-waste crude residual quantity is 0.5-3.0% (W/W). The method is simple to operate, green, friendly to environment and low in cost, resource is recoverable, and the method possesses relatively high environment benefit and economic benefit, and provides new thinking for harmless and resourceful treatment on ground oil soil/ oil sludge/ oil sediment and other oil-containing hazardous wastes.
Description
Technical field
The present invention relates to a kind of method utilizing the oil-containing Hazardous wastes Crude Oils such as microbial technique refiltered oil soil, greasy filth and bed mud, belong to reclamation of solid wastes processing technology field.
Background technology
Produce a large amount of oily solid sludge at oil production, collection, transport and storage process, crude content is usually even higher about 10%.These oily solid sludges not only have the combustibility of height, and also show certain bio-toxicity due to the existence of benzene class, phenols and anthracene class material, endanger surrounding body, soil and environment structure.This kind of oily solid sludge is classified as Hazardous wastes by China, and it is collected, transport, store and disposal is subject to strict supervision.Oil-containing danger is useless mainly comprises landing oil soil, exploitation greasy filth and storage tank bed mud etc.Often exploit about 200,000 tons of the oil-containing solid waste that ten million ton of crude oil produces.China produces oil per year and surpasses hundred million tons, the micro-useless amount incomplete statistics more than 3,000,000 tons of oil-containing.
By regulation, Hazardous wastes must carry out harmless treatment by having the unit of qualification, and Wei Fei manufacturing enterprise needs to pay corresponding disposal expense.But due to history, the method for landfill after early stage simple landfill or solidification is still continued to use in the useless process of China oil-containing danger mostly, there is serious security risk and environmental hazard, does not realize innoxious object.Although high temperature incineration technique can meet the useless harmless treatment of oil-containing danger, too high disposal expense brings huge economical load to Wei Fei manufacturing enterprise, causes the serious waste of crude resources and the destruction completely of matrix soil simultaneously.Therefore, no matter from environment protection or the angle of energy recovery, the recycling treatment reclaiming crude oil to greatest extent represents the developing direction of the useless disposal technology of oil-containing danger.
Utilizing organic solvent be separated crude oil and soil and then complete former oil recovery, is the main technique of the useless recycling treatment of current oil-containing danger.Although this technique can realize the efficient of crude oil and reclaim fast, a large amount of uses of organic solvent cause System risk to significantly improve, and operational condition is also quite harsh; And the regeneration of organic solvent needs pyrogenic distillation, energy consumption is large, cost is high, process is complicated.Green, safety, economy, the efficiently useless resource process new technology of oil-containing danger are are urgently researched and developed.
Microbial technique is introduced the useless recycling treatment of oil-containing danger by the present invention, realizes oil-containing danger useless Central Plains oil recovery under the mild conditions of normal temperature and pressure.Its principle is: special efficacy microorganism species take crude oil as carbon source and energy decomposition long chain molecule and synthetic surfactant.Long chain molecule shortens the cohesive force reduced between crude oil and earth, and the generation of tensio-active agent further promotes the separation between crude oil and earth.Making oily native Crude Oil content be down to 1.0%-4.0% by about 5.0-20% by crude oil recovery, achieve the crude oil recovery rate of 70-90%, laying a good foundation for meeting 0.3% advanced treatment that is agricultural or 0.5% work standard.
Summary of the invention
The present invention seeks to, in order to give up carry out recycling treatment to landing oil soil, danger such as oil-containing such as exploitation greasy filth and storage tank bed mud etc., to propose a kind of microbial process of the recovery crude oil based on hardly biodegradableorganic.The object of the invention is to be achieved through the following technical solutions.
The present invention a kind ofly utilizes microbial technique to reclaim crude oil thus realizes the method for the useless recycling treatment of oil-containing danger, and concrete steps are as follows:
Step one, the cultivation of high efficiency flora
For crude oil recovery high effective microbial community from the oil-polluted soils of various places and bed mud seed selection composite.Comprise Alkanindigessp. (45%), Acinetobactersp. (30%), Pseudomonassp. (15%), Cupriavidussp. (5%) and Arthrobactersp. (5%).With oil-containing Solution culture method high efficiency flora, nutrient solution prescription: NH
4nO
3or KNO
3or NH
4cI or (NH
4)
2sO
4, 0.4 ~ 2.0g/L; Na
2hPO
4or KH
2pO
4, 0.4 ~ 2.0g/L; NaCl, 0.2 ~ 1.0g/L; MgSO
4, 0.2 ~ 1.0g/L; CaCl
2, 0.01 ~ 0.05g/L; FeSO
4, 0.01 ~ 0.05g/L; Crude oil, 10 ~ 50g/L; PH6.0-8.0.By supersound process or add a certain amount of Tweens tensio-active agent and make crude oil form homodisperse emulsion.With distilled water or tap water preparation nutritive medium.In shaking table or aeration and biological fermentor tank, cultivate flora, condition is as follows: temperature 20-40 degree, shaking table or mixing speed 40-200 rev/min, aeration rate 0.1-0.5 times of nutritive medium volume/minute.After cultivating about 2-4 days, Growth of Cells reaches stationary phase, cell turbidity 300-1200FTU.High efficiency flora suspension can be used for bioseparation and the recovery of the useless Crude Oil of oil-containing danger.
Step 2, by useless for the oil-containing such as oil soil, greasy filth, bed mud the danger broken and foreign material such as removing stone, wooden unit, cloth, plastics and stalk that sieve, to store for future use;
The microorganism of the useless Crude Oil of step 3, oil-containing danger is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: NH
4nO
3or KNO
3or NH
4cI or (NH
4)
2sO
4, 0.1 ~ 1.0g/L; Na
2hPO
4or KH
2pO
4, 0.1 ~ 1.0g/L; NaCl, 0.1 ~ 0.5g/L; MgSO
4, 0.1 ~ 0.5g/L; CaCl
2, 0.01 ~ 0.05g/L; FeSO
4, 0.01 ~ 0.05g/L; PH6.0-8.0.Bioseparation and the recovery of the useless Crude Oil of oil-containing danger is realized in mud reactor.Mud system is endangered useless by oil-containing and mineral nutrition liquid is formulated by a certain percentage, mineral nutrition liquid: the useless ratio of oil-containing danger is 3:1-6:1 (V/V), high efficiency flora inoculum size is 2%-10%, temperature of reaction is 20-40 degree, aeration rate be 0.05-0.5 times of nutritive medium volume/minute, mixing speed is 40-200 rev/min.Run after 3-9 days, the useless Crude Oil of oil-containing danger is separated with substrate and acts on float downward to reactor liquid level at aeration (air supporting), crude oil recovery rate 60-90%, solid waste crude oil residual quantity 0.5-3.0% (W/W).Operational process is looked flora growth situation and is suitably supplemented microbial inoculum.Mud reactor volume 1-500 cube, tool heating, aeration and whipping appts (see photo).
The present invention adopts the beneficial effect of above technical scheme
The present invention is based on the Microbial resources treatment technology that mud reactor reclaims crude oil from oil-containing danger is useless, than pyrolysis method and solidification method, there is resource reclaim advantage and good economic benefit undoubtedly, reclaim rule than organic solvent washing and show green, environmental protection, safe and economic feature.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Accompanying drawing is the structural representation of bio-reactor of the present invention and mud reactor.
In figure, 1 is motor, and 2 is venting ports, and 3 is circulating water outlets, and 4 is pH, Do probes, and 5 is aerating apparatuss, and 6 is discharge ports, and 7 is dog-houses, and 8 is agitators, and 9 is heating in water bath water-ins
Embodiment
Below in conjunction with examples of implementation, the present invention is described in detail.
Embodiment 1
1. the cultivation of high efficiency flora
High efficiency flora comprises Alkanindigessp., Acinetobactersp., Pseudomonassp., Cupriavidussp. and Arthrobactersp..Nutrient solution prescription: NH4NO3,1.0g/L; Na2HPO4,1.0g/L; NaCl, 0.5g/L; MgSO4,0.5g/L; CaCl2,0.05g/L; FeSO4,0.01g/L; Crude oil, 20g/L; PH7.2.Add 1.0% (v/v) polysorbas20 fully to stir and make crude oil form emulsion evenly spreading in nutrient solution.Nutritive medium is prepared with tap water.In 10 liters of biological fermentation tanks, cultivate flora, condition is as follows: temperature 35 degree, mixing speed 80 revs/min, aeration rate 2 liters/min.Cultivate after 4 days, cell turbidity reaches 600FTU, and high efficiency flora seed liquor is for subsequent use.
Step 2, by broken for oil soil and for subsequent use after the foreign material such as removing stone, wooden unit, cloth, plastics and stalk that sieve;
The microorganism of step 3, oily native Crude Oil is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: KNO3,0.5g/L; KH2PO4,0.5g/L; NaCl, 0.25g/L; MgSO4,0.25g/L; CaCl2,0.01g/L; FeSO4,0.01g/L; PH7.0.Bioseparation and the recovery of crude oil is realized in 100 liters of mud reactors.Mud system by oil soil and mineral nutrition liquid formulated, mineral nutrition liquid: oily native ratio is 4:1 (V/V), and high efficiency flora is inoculum size 10% first, temperature of reaction 35 degree, aeration rate 20 liters/min, mixing speed 120 revs/min.Run duration, collects the crude oil once floating on liquid level, and adds high efficiency flora 5% (v/v) for every two days.Run after 5 days, crude oil adds up the rate of recovery 80%, solid waste crude oil residual quantity 1.7% (W/W).The crude oil reclaimed directly weighs to calculate the rate of recovery; The heavy method of difference is adopted to measure in oil soil after the extraction of residual quantity organic reagent.
Embodiment 2
1. the cultivation of high efficiency flora
High efficiency flora comprises Alkanindigessp., Acinetobactersp., Pseudomonassp., Cupriavidussp. and Arthrobactersp..Nutrient solution prescription: NH
4nO
3, 1.0g/L; Na
2hPO
4, 1.0g/L; NaCl, 0.5g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.01g/L; Crude oil, 20g/L; PH7.2.Add 1.0% (v/v) polysorbas20 fully to stir and make crude oil form emulsion evenly spreading in nutrient solution.Nutritive medium is prepared with tap water.In 10 liters of biological fermentation tanks, cultivate flora, condition is as follows: temperature 35 degree, mixing speed 80 revs/min, aeration rate 2 liters/min.Cultivate after 4 days, cell turbidity reaches 600FTU, and high efficiency flora seed liquor is for subsequent use.
Step 2, oil-mud disintegrating is after the foreign material such as removing stone, wooden unit, cloth, plastics and stalk etc. that sieve for subsequent use;
The microorganism of step 3, greasy filth Crude Oil is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: KNO
3, 0.5g/L; KH
2pO
4, 0.5g/L; NaCl, 0.25g/L; MgSO
4, 0.25g/L; CaCl
2, 0.01g/L; FeSO
4, 0.01g/L; PH7.0.Bioseparation and the recovery of crude oil is realized in 100 liters of mud reactors.Mud system by oil soil and mineral nutrition liquid formulated, mineral nutrition liquid: oily native ratio is 4:1 (V/V), and high efficiency flora is inoculum size 10% first, temperature of reaction 35 degree, aeration rate 20 liters/min, mixing speed 120 revs/min.Run duration, collects the crude oil once floating on liquid level, and adds high efficiency flora 5% (v/v) for every two days.Run after 7 days, crude oil adds up the rate of recovery 72%, solid waste crude oil residual quantity 2.3% (W/W).The crude oil reclaimed directly weighs to calculate the rate of recovery; The heavy method of difference is adopted to measure in greasy filth after the extraction of residual quantity organic reagent.
Embodiment 3
1. the cultivation of high efficiency flora
High effective microbial community comprises Alkanindigessp., Acinetobactersp., Pseudomonassp., Cupriavidussp. and Arthrobactersp..With oil-containing Solution culture method high efficiency flora, nutrient solution prescription: KNO
3, 2.0g/L; Na
2hPO
4, 2.0g/L; NaCl, 1.0g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.01g/L; Crude oil, 30g/L; PH7.0.Supersound process makes crude oil form homodisperse emulsion for two hours.Nutritive medium is prepared with tap water.In 100 liters of aeration and biological fermentor tanks, cultivate flora, condition is as follows: temperature 30 degree, mixing speed 100 revs/min, aeration rate 25 liters/min.Cultivate after 3 days, cell turbidity 750FTU.High efficiency flora suspension is used for follow-up crude separation and reclaims.
2., by broken for oil soil and the foreign material such as removing stone, wooden unit, cloth, plastics and stalk that sieve, store for future use;
3. the microorganism of oily native Crude Oil is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: KNO
3, 0.5g/L; NH
4cI, 0.5g/L; Na
2hPO
4, 0.5g/L; KH
2pO
4, 0.5g/L; NaCl, 0.5g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.05g/L; PH7.0.Bioseparation and the recovery of crude oil is realized in 1000 liters of mud reactors.Mud system by oil soil and mineral nutrition liquid formulated, nutritive medium: oily native ratio is 5:1 (V/V), and high efficiency flora inoculum size is 8.0%, and temperature of reaction is 30 degree, and aeration rate is 100 liters/min, and mixing speed is 120 revs/min.Run duration, collects the crude oil once floating on liquid level, and adds high efficiency flora 5% (v/v) for every two days.Run after 5 days, crude oil adds up the rate of recovery 78%, solid waste crude oil residual quantity 1.8% (W/W).The crude oil reclaimed directly weighs to calculate the rate of recovery; The heavy method of difference is adopted to measure in greasy filth after the extraction of residual quantity organic reagent.
Embodiment 4
1. the cultivation of high efficiency flora
High effective microbial community comprises Alkanindigessp., Acinetobactersp., Pseudomonassp., Cupriavidussp. and Arthrobactersp..With oil-containing Solution culture method high efficiency flora, nutrient solution prescription: KNO
3, 2.0g/L; Na
2hPO
4, 2.0g/L; NaCl, 1.0g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.01g/L; Crude oil, 30g/L; PH7.0.Supersound process makes crude oil form homodisperse emulsion for two hours.Nutritive medium is prepared with tap water.In 100 liters of aeration and biological fermentor tanks, cultivate flora, condition is as follows: temperature 30 degree, mixing speed 100 revs/min, aeration rate 25 liters/min.Cultivate after 3 days, cell turbidity 750FTU.High efficiency flora suspension is used for follow-up crude separation and reclaims.
2., by oil-mud disintegrating and the foreign material such as removing stone, wooden unit, cloth, plastics and stalk etc. that sieve, store for future use;
3. the microorganism of greasy filth Crude Oil is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: KNO
3, 0.5g/L; NH
4cI, 0.5g/L; Na
2hPO
4, 0.5g/L; KH
2pO
4, 0.5g/L; NaCl, 0.5g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.05g/L; PH7.0.Bioseparation and the recovery of crude oil is realized in 1000 liters of mud reactors.Mud system by greasy filth and mineral nutrition liquid formulated, nutritive medium: greasy filth ratio is 5:1 (V/V), and high efficiency flora inoculum size is 8.0%, and temperature of reaction is 30 degree, and aeration rate is 100 liters/min, and mixing speed is 150 revs/min.Run duration, collects the crude oil once floating on liquid level, and adds high efficiency flora 5% (v/v) for every two days.Run after 7 days, crude oil adds up the rate of recovery 70%, solid waste crude oil residual quantity 2.5% (W/W).The crude oil reclaimed directly weighs to calculate the rate of recovery; The heavy method of difference is adopted to measure in greasy filth after the extraction of residual quantity organic reagent.
Embodiment 5
1. the cultivation of high efficiency flora
High effective microbial community comprises Alkanindigessp., Acinetobactersp., Pseudomonassp., Cupriavidussp. and Arthrobactersp..With oil-containing Solution culture method high efficiency flora, nutrient solution prescription: KNO
3, 2.0g/L; Na
2hPO
4, 2.0g/L; NaCl, 1.0g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.01g/L; Crude oil, 15g/L; PH7.0.Add 0.75% (v/v) tween 80 fully to stir and supersound process makes crude oil form emulsion evenly spreading in nutrient solution for one hour.Nutritive medium is prepared with tap water.In 500 liters of aeration and biological fermentor tanks, cultivate flora, condition is as follows: temperature 35 degree, mixing speed 100 revs/min, aeration rate 50 liters/min.Cultivate after 4 days, cell turbidity 450FTU.High efficiency flora suspension is used for follow-up crude separation and reclaims.
2., by broken for oil soil and the foreign material such as removing stone, wooden unit, cloth, plastics and stalk that sieve, store for future use;
3. the microorganism of oily native Crude Oil is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: KNO
3, 0.5g/L; NH
4cI, 0.5g/L; Na
2hPO
4, 0.5g/L; KH
2pO
4, 0.5g/L; NaCl, 0.5g/L; MgSO
4, 0.5g/L; CaCl
2, 0.05g/L; FeSO
4, 0.05g/L; PH7.0.Bioseparation and the recovery of crude oil is realized in 5000 liters of mud reactors.Mud system by oil soil and mineral nutrition liquid formulated, nutritive medium: oily native ratio is 6:1 (V/V), and high efficiency flora inoculum size is 6.0%, and temperature of reaction is 30 degree, and aeration rate is 500 liters/min, and mixing speed is 150 revs/min.Run duration, collects the crude oil once floating on liquid level, and adds high efficiency flora 5% (v/v) for every two days.Run after 7 days, crude oil adds up the rate of recovery 79%, solid waste crude oil residual quantity 1,7% (W/W).The crude oil reclaimed directly weighs to calculate the rate of recovery; The heavy method of difference is adopted to measure in greasy filth after the extraction of residual quantity organic reagent.
Claims (2)
1. utilize microbial technique to reclaim crude oil thus realize the method for the useless recycling treatment of oil-containing danger, it is characterized in that the step of the method:
Step one, the cultivation of high efficiency flora
For crude oil recovery high effective microbial community from the oil-polluted soils of various places and bed mud seed selection composite.Comprise Alkanindigessp. (45%), Acinetobactersp. (30%), Pseudomonassp. (15%), Cupriavidussp. (5%) and Arthrobactersp. (5%).With oil-containing Solution culture method high efficiency flora, nutrient solution prescription: NH
4nO
3or KNO
3or NH
4cI or (NH
4)
2sO
4, 0.4 ~ 2.0g/L; Na
2hPO
4or KH
2pO
4, 0.4 ~ 2.0g/L; NaCl, 0.2 ~ 1.0g/L; MgSO
4, 0.2 ~ 1.0g/L; CaCl
2, 0.01 ~ 0.05g/L; FeSO
4, 0.01 ~ 0.05g/L; Crude oil, 10 ~ 50g/L; PH6.0-8.0.By supersound process or add a certain amount of Tweens tensio-active agent and make crude oil form homodisperse emulsion.With distilled water or tap water preparation nutritive medium.In shaking table or aeration and biological fermentor tank, cultivate flora, condition is as follows: temperature 20-40 degree, shaking table or mixing speed 40-200 rev/min, aeration rate 0.1-0.5 times of nutritive medium volume/minute.After cultivating about 2-4 days, Growth of Cells reaches stationary phase, cell turbidity 300-1200FTU.High efficiency flora suspension can be used for bioseparation and the recovery of the useless Crude Oil of oil-containing danger.
Step 2, by useless for the oil-containing such as oil soil, greasy filth, bed mud the danger broken and foreign material such as removing stone, wooden unit, cloth, plastics and stalk that sieve, to store for future use;
The microorganism of the useless Crude Oil of step 3, oil-containing danger is separated and reclaims
Preparation mineral nutrition liquid, fills a prescription as follows: NH
4nO
3or KNO
3or NH
4cI or (NH
4)
2sO
4, 0.1 ~ 1.0g/L; Na
2hPO
4or KH
2pO
4, 0.1 ~ 1.0g/L; NaCl, 0.1 ~ 0.5g/L; MgSO
4, 0.1 ~ 0.5g/L; CaCl
2, 0.01 ~ 0.05g/L; FeSO
4, 0.01 ~ 0.05g/L; PH6.0-8.0.Bioseparation and the recovery of the useless Crude Oil of oil-containing danger is realized in mud reactor.Mud system is endangered useless by oil-containing and mineral nutrition liquid is formulated by a certain percentage, mineral nutrition liquid: the useless ratio of oil-containing danger is 3:1-6:1 (V/V), high efficiency flora inoculum size is 2%-10%, temperature of reaction is 20-40 degree, aeration rate be 0.05-0.5 times of nutritive medium volume/minute, mixing speed is 40-200 rev/min.Run after 3-9 days, the useless Crude Oil of oil-containing danger is separated with substrate and acts on float downward to reactor liquid level at aeration (air supporting), crude oil recovery rate 60-90%, solid waste crude oil residual quantity 0.5-3.0% (W/W).Operational process is looked flora growth situation and is suitably supplemented microbial inoculum (2%-10%) (v/v).
2. the method with microbial technique recycling treatment landing oil soil/greasy filth/bed mud.It is characterized in that:
It with the crude oil in oily soil/greasy filth/bed mud for carbon source and the energy, and can not only be separated with substrate by selected microorganism species, realizes former oil recovery and the useless recycling treatment of oil-containing danger.
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