CN105434323B - Saccharomycetes to make fermentation compound and its application in skin whitening, moisturizing skin care item - Google Patents
Saccharomycetes to make fermentation compound and its application in skin whitening, moisturizing skin care item Download PDFInfo
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- CN105434323B CN105434323B CN201510980162.0A CN201510980162A CN105434323B CN 105434323 B CN105434323 B CN 105434323B CN 201510980162 A CN201510980162 A CN 201510980162A CN 105434323 B CN105434323 B CN 105434323B
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- saccharomycetes
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- jasmine flower
- make fermentation
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- XMLSXPIVAXONDL-UHFFFAOYSA-N trans-jasmone Natural products CCC=CCC1=C(C)CCC1=O XMLSXPIVAXONDL-UHFFFAOYSA-N 0.000 description 1
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of saccharomycetes to make fermentation compound and its applications in skin whitening, moisturizing skin care item.The saccharomycetes to make fermentation compound is to add in Rhizoma Polygonati Odorati extract and jasmine flower extract in yeast culture medium, the zymotic fluid obtained after being fermented with saccharomycete.The composition clear appearance face light yellow complexion, smell uniqueness have the typical fragrance of jasmine and radix polygonati officinalis concurrently, and fragrance enriches pleasant.Present invention discover that saccharomycetes to make fermentation compound significantly inhibits tyrosinase activity effect and good moisture-keeping function.The fermentation compound can be used as adding ingredient, it is added into cosmetics according to method known in cosmetics industry and external preparation is made, such as solution, suspension, cream kind, type of latex type, gel-like, powder, spray State of cosmetics, so as to achieve the effect that skin whitening, moisturizing.
Description
Technical field
The invention belongs to microbial fermentation technology and cosmetic technical field, more particularly to a kind of saccharomycetes to make fermentation compound group
Close object and its application in skin whitening, moisturizing skin care item.
Background technology
The yeast extract generated after yeast fermentation contains abundant amino acid, polypeptide, various vitamin and minerals, this
A little substances play indispensable effect in the treatment of skin, and the work(such as preferable whitening, moisturizing can be played by different approaches
Effect.But yeast fermentation broth smell generally has vinasse taste or special fermentation taste, and part population does not receive this yeast water taste
Road, perfumer can add in compound essence according to cosmetic product feature or positioning and carry out fragrance adjustment or cover.But the often later stage
Add in smell after this compound essence it is not pure and mild enough or have increase irritation may, cause product effect undesirable.
Cosmetics add essence at present, the production of fragrance is mainly artificial synthesized and is extracted from natural animal-plant.By
The safety of artificial synthesized essence, fragrance is often worried in people, so being more likely to receive to obtain from natural animal-plant kind
Essence, spice material.Jasmine mostly extracts cream jasmin france by supercritical process extraction or organic solvent at present, although efficiently
Simplicity, but such as alcohol organic solvent is often remained, irritation easily is come to delicate skin tape.
Invention content
The shortcomings that primary and foremost purpose of the present invention is to overcome the prior art and deficiency, provide a kind of saccharomycetes to make fermentation compound group
Close object.
Another object of the present invention is to provide the saccharomycetes to make fermentation compound in skin whitening, moisturizing skin care item
Using.
The purpose of the present invention is achieved through the following technical solutions：A kind of saccharomycetes to make fermentation compound, is to carry radix polygonati officinalis
Object and jasmine flower extract is taken to add in yeast culture medium, the zymotic fluid obtained after being fermented with saccharomycete.
The saccharomycete must can Candida (Candida lambica) CGMCC 2.1763 and saccharomyces cerevisiae for youth
It is one or two kinds of in (Saccharomyces cerevisiae) CGMCC 2.1.
The Rhizoma Polygonati Odorati extract is water extract.
The jasmine flower extract is water extract.
The Rhizoma Polygonati Odorati extract and the jasmine flower extract in mass ratio 0.01:1~100：1 proportioning；Preferably
By 1：1~20：1 proportioning.
The yeast culture medium is to be suitble to the culture medium of Yeast Growth breeding.
The condition of the fermentation is to be suitble to the condition of Yeast Growth breeding；It is it is preferred that as follows：PH value is 4.0~7.0, is stirred
Speed is mixed as 60~250rpm, ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, and fermentation time is 2 days~4 days.
The Rhizoma Polygonati Odorati extract is prepared preferably by following steps：Fragrant Solomonseal Rhizome and water are mixed, ultrasound carries
It takes, extraction is boiled in then heating, is concentrated, is obtained Rhizoma Polygonati Odorati extract.
The condition of the ultrasonic extraction is preferably extracted 30~60 minutes in 50~60 DEG C, and ultrasonic power is preferably 100
~500W, more preferably 100~300W.
The jasmine flower extract is prepared preferably by following steps：Dried jasmine flower and water are mixed, heating
Infiltration, filtering, obtains jasmine flower extract.
The condition of the heating infiltration is preferably 70~90 DEG C of 2~4h of infiltration.
The preparation method of the saccharomycetes to make fermentation compound, comprises the following steps：
(1) Fragrant Solomonseal Rhizome and water are mixed, ultrasonic extraction, extraction is boiled in then heating, is concentrated, is obtained Rhizoma Polygonati Odorati extract；
(2) dried jasmine flower and water are mixed, heating infiltration, filtering obtains jasmine flower extract；
(3) above-mentioned Rhizoma Polygonati Odorati extract with jasmine flower extract is mixed, stirred evenly, filtered, degerming obtains mixture；
Mixture with by the yeast culture medium of bacteria removing is mixed, obtains fermentation medium；
(4) saccharomycete is inoculated into fermentation medium and fermented；
(5) the Preliminary fermentation liquid obtained after fermentation is subjected to separation of solid and liquid, takes liquid, obtain the combination of saccharomycetes to make fermentation compound
Object.
Fragrant Solomonseal Rhizome described in step (1) first pass through in advance clean after pulverize and sieve to obtain.
Water described in step (1) and step (3) is preferably deionized water.
The dosage of water described in step (1) is preferably equivalent to 5~8 times of radix polygonati officinalis quality.
The condition of ultrasonic extraction described in step (1) is preferably in 50~60 DEG C of ultrasonic extractions 30~60 minutes, ultrasound
Power is preferably 100~500W, more preferably 100~300W.
The time that extraction is boiled in heating described in step (1) is preferably 30~90 minutes.
Filtering described in step (1) and step (2) is preferably by 8 layers of filtered through gauze.
The degree of concentration described in step (1) is preferably concentrated to density 1.01~1.20；More preferably 1.05~
1.15。
The dosage of water described in step (2) is preferably equivalent to 5~10 times of dried jasmine flower quality；More preferably 8~10
Times.
The condition of heating infiltration described in step (2) preferably infiltrates 0.5~4h in 70 DEG C~100 DEG C, more preferably
2~4h is infiltrated in 70 DEG C~90 DEG C.
Rhizoma Polygonati Odorati extract and the jasmine flower extract in mass ratio 0.01 described in step (3):1~100：1 matches
Than；Preferably press 1：1~20：1 proportioning.
Filtering described in step (3) is preferably by 0.45 μm of filtering with microporous membrane.
The addition volume of mixture described in step (3) is preferably the 10~20% of the yeast culture medium volume.
Degerming described in step (3) is by 0.2~0.22 μm of filtering with microporous membrane degerming or passes through 110~121 DEG C
Processing sterilizes for 15~30 minutes.
Yeast culture medium described in step (3) is to be suitble to the culture medium of yeast growth breeding, preferably YPDA culture mediums.
Saccharomycete described in step (4) must can 2.1763 Hes of Candida (Candida lambica) CGMCC for youth
It is one or two kinds of in saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC 2.1.
Saccharomycete described in step (4) is preferably in the saccharomycete of exponential phase.
The inoculum concentration of yeast described in step (4) by yeast in the fermentation medium a concentration of 5~8 ×
106Cell/ml meters, preferably 6~8 × 106Cell/ml.
The condition of fermentation described in step (4) is preferably as follows：PH value is 4.0~7.0, mixing speed for 60~
250rpm, ventilatory capacity are 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, and fermentation time is 2 days~4 days.
Separation of solid and liquid described in step (5) is carried out preferably by following steps：Supernatant, supernatant are obtained by seperator
Saccharomycete is removed by 0.2~0.22 μm of filtering with microporous membrane again.
The saccharomycetes to make fermentation Chinese medicine composition containing skin-care effect traditional Chinese medicine ingredients obtained as stated above, which can be used as, to be added
Addition point, the production applied to cosmetics.Can according to method known in cosmetics industry will the present invention saccharomycetes to make fermentation in
Drug composition, which is added into cosmetics, is made external preparation, such as solution, suspension, cream kind, type of latex type, gel-like, powder, spray
Wait State of cosmetics.
Application of the saccharomycetes to make fermentation compound in skin whitening, moisturizing skin care item are prepared.
Application of the saccharomycetes to make fermentation compound in skin whitening, moisturizing skin care item are prepared, wherein, the yeast
A concentration of mass percent 0~100% of the bacterium fermentation compound in the skin whitening, moisturizing skin care item, without endpoint value
0 and 100%；Preferably mass percent 1~30%.
The skin whitening, moisturizing skin care item can also contain whitening agent, moisturizer, antioxidant, surfactant, alcohols
One kind in compound and water or at least two.
A kind of skin whitening, moisturizing skin care item contain above-mentioned saccharomycetes to make fermentation compound.
The present invention is had the following advantages relative to the prior art and effect：
(1) radix polygonati officinalis has yin-nourishing, moisturizes, relieving restlessness, quenches the thirst and other effects.Radix polygonati officinalis exists《Compendium of Materia Medica》In be referred to as top grade, be
Enriching yin panacea, the demulcen of medicine-food two-purpose.Modern medicine study also indicates that radix polygonati officinalis is rich in protein, crude fibre, niacin, the lily of the valley
Glucoside, convallamarin, Kaempferol, Quercetin, mucilaginous substance, carbohydrate, vitamin A, zinc, manganese etc..Skin nourishment, whitening are made
With apparent.Jasmine, it is pungent, sweet, cool, have clearing heat and detoxicating, dampness removing effect.Benzyl alcohol is mainly contained in Jasmine volatile materials
Or its lipid, jasmone, linalool, benzoic acid linalool ester, jasmine lactone etc..With skin is improved, moisturizing skin is moistened
The effect of skin, while pleasant aroma are easy to that people is allowed to receive.The present invention is directly leached with water from Jasmine, radix polygonati officinalis plant cell
Active ingredient, using biofermentation technique life it is allowed further to be coexisted with saccharomycete, it is found that it does not have inhibition to saccharomycete,
Yeast cometabolism biosynthesis pathway may be stimulated to change instead, so as to change fermented liquid smell, substantially reduced after fermentation
Yeast fermentation broth is original vinasse taste or special fermentation taste, overcomes current yeast fermentation broth smell bad or adds in
The defects of irritation increase is easy to cause after cream jasmin france.Yeast provided by the invention fermentation compound clear appearance, face
Color is colourless or slightly micro- Huang, and smell uniqueness has the typical fragrance of jasmine and radix polygonati officinalis concurrently, and fragrance enriches pleasant.While micro- life of fermenting
Object effect is lower to make the active principle contained by radix polygonati officinalis further convert, as saponins macromolecular substances are converted into smaller sapogenin
Molecule etc. makes nourishing yin effect stronger, skin is acted on more prominent.Result of the present invention also finds in surprise, Jasmine extracting solution, radix polygonati officinalis
Extracting solution handles the fermentation composition prepared through saccharomycete co-fermentation, is added in cosmetics, is remarkably improved cosmetics U.S.
White moisture-keeping efficacy has the function of more detailed nourishing, skin whitening.
(2) present invention provides a kind of new developing direction for Cosmetic Market.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 5 times of quality of radix polygonati officinalis, 50 DEG C of ultrasounds
(120W) is extracted 30 minutes, and extraction 60 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.08 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:8 ratio mixing, in 80 DEG C of infiltration extraction 2h, 8 layers of gauze
Filtering, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 1:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 20 minutes through 121 DEG C, cooled to room temperature.
(4) the good saccharomyces cerevisiae of advance shaking table culture (Saccharomyces cerevisiae) CGMCC 2.1 is inoculated with
Into yeast complete medium (YPDA culture mediums), the mixture that step (3) obtains, the addition volume phase of mixture are added
When in the 1/10 of yeast complete medium volume, it is 8 × 10 to make yeast concentration6Cell/ml, ferments, fermentation parameter
It is 4.0~7.0 for pH value, mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, hair
The ferment time is 3 days.
(5) the Preliminary fermentation liquid seperator obtained after fermentation (butterfly seperator, at full speed 6500 revs/min) is divided
From collection separation supernatant is saccharomycetes to make fermentation compound through 0.2 μm of filtering with microporous membrane.The composition clear appearance
Color is colourless, and smell uniqueness has the typical fragrance of jasmine and radix polygonati officinalis concurrently, and fragrance enriches pleasant.
Embodiment 2
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 5 times of quality of radix polygonati officinalis, 60 DEG C of ultrasounds
(120W) is extracted 60 minutes, and extraction 60 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.11 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:10 ratio mixing, infiltrates 4h, 8 layers of gauze mistake in 70 DEG C
Filter, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 5:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 30 minutes through 121 DEG C, cooled to room temperature.
(4) the good saccharomyces cerevisiae of advance shaking table culture (Saccharomyces cerevisiae) CGMCC 2.1 is inoculated with
Into yeast complete medium (YPDA culture mediums), the mixture that step (3) obtains, the addition volume phase of mixture are added
When in the 1/5 of yeast complete medium volume, it is 7 × 10 to make yeast concentration6Cell/ml, ferments, and fermentation parameter is
PH value is 4.0~7.0, and mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, fermentation
Time is 3 days.
(5) the Preliminary fermentation liquid obtained after fermentation with butterfly seperator is detached, separation supernatant is collected, through 0.2 μ
M filtering with microporous membrane is saccharomycetes to make fermentation compound.
Embodiment 3
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 8 times of quality of radix polygonati officinalis, 50 DEG C of ultrasounds
(200W) is extracted 30 minutes, and extraction 90 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.15 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:8 ratio mixing, infiltrates 3h, 8 layers of gauze mistake in 90 DEG C
Filter, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 10:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 15 minutes through 115 DEG C, cooled to room temperature.
(4) by the good youth of advance shaking table culture must can Candida (Candida lambica) CGMCC 2.1763 be inoculated with
Into yeast complete medium (YPDA culture mediums), the mixture that step (3) obtains, the addition volume phase of mixture are added
When in the 15% of yeast complete medium volume, it is 8 × 10 to make yeast concentration6Cell/ml, ferments, fermentation parameter
It is 4.0~7.0 for pH value, mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, hair
The ferment time is 2 days.
(5) the Preliminary fermentation liquid obtained after fermentation with butterfly seperator is detached, separation supernatant is collected, through 0.2 μ
M filtering with microporous membrane obtains saccharomycetes to make fermentation compound.
Embodiment 4
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 6 times of quality of radix polygonati officinalis, 60 DEG C of ultrasounds
(200W) is extracted 60 minutes, and extraction 90 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.10 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:10 ratio mixing, infiltrates 4h, 8 layers of gauze mistake in 70 DEG C
Filter, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 15:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 20 minutes through 121 DEG C, cooled to room temperature.
(4) by the good youth of advance shaking table culture must can Candida (Candida lambica) CGMCC 2.1763 be inoculated with
Into yeast complete medium (YPDA culture mediums), the mixture that step (3) obtains, the addition volume phase of mixture are added
When in the 1/5 of yeast complete medium volume, it is 8 × 10 to make yeast concentration6Cell/ml, ferments, and fermentation parameter is
PH value is 4.0~7.0, and mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, fermentation
Time is 4 days.
(5) the Preliminary fermentation liquid obtained after fermentation with butterfly seperator is detached, separation supernatant is collected, through 0.2 μ
M filtering with microporous membrane obtains saccharomycetes to make fermentation compound.
Embodiment 5
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 6 times of quality of radix polygonati officinalis, 60 DEG C of ultrasounds
(300W) is extracted 30 minutes, and extraction 30 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.08 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:8 ratio mixing, infiltrates 2h, 8 layers of gauze mistake in 80 DEG C
Filter, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 20:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 10 minutes through 115 DEG C, cooled to room temperature.
(4) the good saccharomyces cerevisiae of advance shaking table culture (Saccharomyces cerevisiae) CGMCC 2.1 is inoculated with
Into yeast complete medium (YPDA culture mediums), the mixture that step (3) obtains, the addition volume phase of mixture are added
When in the 30% of yeast complete medium volume, it is 6 × 10 to make yeast concentration6Cell/ml, ferments, fermentation parameter
It is 4.0~7.0 for pH value, mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, hair
The ferment time is 2 days.
(5) the Preliminary fermentation liquid obtained after fermentation with butterfly seperator is detached, separation supernatant is collected, through 0.2 μ
M filtering with microporous membrane obtains saccharomycetes to make fermentation compound.
Reference examples 1
(1) by dried jasmine flower and deionized water in mass ratio 1:10 ratio infiltrates 2h in 80 DEG C, and 8 layers of filtered through gauze obtain
Filtrate.
(2) the good saccharomyces cerevisiae of advance shaking table culture (Saccharomyces cerevisiae) CGMCC 2.1 is inoculated with
Into yeast complete medium, the mixture that step (3) obtains is added, it is complete that the addition volume of mixture is equivalent to yeast
The 1/10 of culture volume, it is 6 × 10 to make yeast concentration6Cell/ml, ferments, fermentation parameter be pH value be 4.0~
7.0, mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, and fermentation time is 2 days.
(3) the Preliminary fermentation liquid obtained after fermentation with seperator is detached, collects separation supernatant, it is micro- through 0.2 μm
Hole membrane filtration obtains saccharomycetes to make fermentation compound.
Reference examples 2
(1) by the good youth of advance shaking table culture must can Candida (Candida lambica) CGMCC 2.1763 be inoculated with
Into yeast complete medium, it is 6 × 10 to make yeast concentration6Cell/ml, ferments, and fermentation parameter is that pH value is 4.0
~7.0, mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C, fermentation time 2
My god.
(2) the Preliminary fermentation liquid obtained after fermentation with seperator is detached, collects separation supernatant, it is micro- through 0.2 μm
Hole membrane filtration, obtains saccharomycetes to make fermentation liquid.
Reference examples 3
(1) radix polygonati officinalis is cleaned, crushed 60 mesh sieve, add in the deionized water for being equivalent to 5 times of quality of radix polygonati officinalis, 50 DEG C of ultrasounds
(120W) is extracted 30 minutes, and extraction 60 minutes is boiled in then heating, and 8 layers of filtered through gauze are concentrated to density 1.08 (in temperature 60 C
When measure to obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by dried jasmine flower and deionized water in mass ratio 1:8 ratio mixing, in 80 DEG C of infiltration extraction 2h, 8 layers of gauze
Filtering, obtains filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and jasmine flower extract in mass ratio 1:1 mixing, stirs evenly, micro- through 0.45 μm
Hole membrane filtration sterilizes 20 minutes through 121 DEG C, and cooled to room temperature obtains Rhizoma Polygonati Odorati extract and jasmine flower extract mixed liquor.
(4) the good saccharomyces cerevisiae of advance shaking table culture (Saccharomyces cerevisiae) CGMCC 2.1 is inoculated with
Into yeast complete medium (YPDA culture mediums), it is 8 × 10 to make yeast concentration6Cell/ml, ferments, zymotechnique ginseng
It is 4.0~7.0 that number, which is pH value, and mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM, and fermentation temperature is 25 DEG C~30 DEG C,
Fermentation time is 3 days.
(5) the Preliminary fermentation liquid obtained after fermentation with seperator is detached, collects separation supernatant, it is micro- through 0.2 μm
Hole membrane filtration obtains zymotic fluid stoste.
(6) by Rhizoma Polygonati Odorati extract in step (3) and jasmine flower extract mixed liquor, the fermentation that step (5) obtains is added
In stoste, the addition volume of mixed liquor is equivalent to that step (5) obtains in step (3) the 1/10 of fermenation raw liquid volume, obtains
Yeast juice compound.
Effect example：
(1) detection of whitening effect：Tyrosinase inhibition test：Test philosophy：Tyrosinase urges DOPA
Change reaction, can generate DOPA quinone, DOPA quinone maximum absorption wavelength is in 475nm, can be with by measuring the absorptance of 475nm wavelength
Reflect the size of the concentration of generation DOPA quinone.When tyrosinase and whitening active ingredients are existed simultaneously in solution, tyrosine
The catalytic activity of enzyme can be by a degree of inhibition, so as to reduce the generation of DOPA quinone.It is compared, can sentenced according to front and rear absorbance
Inhibition level of the whitening active ingredients to tyrosinase activity is determined, so as to assess the white-skinned face function of this kind of whitening composition in vitro.
Reagent：Reagent 1：(phosphate standard buffer solution weighs after 110 DEG C~130 DEG C dry 2~3h PBS respectively
Potassium dihydrogen phosphate (KH2PO4) 3.388g and disodium hydrogen phosphate (Na2HPO4) 3.533g is dissolved in water and is diluted in volumetric flask
1L), pH value 6.86；Reagent 2：DOPA solution (is prepared, mass fraction 0.03%) with PBS；Reagent 3：Tyrosinase solution (is used
PBS is prepared, 200 μ g/ml of concentration)；Reagent 4：Saccharomycetes to make fermentation compound sample solution (embodiment and reference examples, it is identical
Extension rate).
Experimental method：A test specimens：3ml PBS+0.5ml tyrosinase solutions；B test specimens：PBS；C test specimens：1ml samples
Product solution+2ml PBS+0.5ml tyrosinase solutions；D test specimens：1ml saccharomycetes to make fermentation compounds sample solution+
2.5ml PBS.A, DOPA solution 0.5ml is added after B, C, D test sample liquid prepare simultaneously respectively immediately, it is anti-in 25 DEG C of constant temperature
5min is answered, measures the absorbance value at 475nm.Every group of experiment is parallel to be repeated 3 times, and is averaged.
The computational methods of tyrosinase inhibition rate：
Tyrosinase inhibition rate %=[(A-B)-(C-D)]/(A-B) × 100
The measurement result of tyrosinase inhibition test, as shown in table 1：
Table 1
| Inhibiting rate | |
| Embodiment 1 | 53.72% |
| Embodiment 2 | 56.10% |
| Embodiment 3 | 55.84% |
| Embodiment 4 | 54.37% |
| Embodiment 5 | 55.92% |
| Reference examples 1 | 40.25% |
| Reference examples 2 | 37.18% |
| Reference examples 3 | 44.92% |
(2) detection of moistening effect：Keratoderma hydration rate is tested：Test philosophy be using capacitor as instrument probe,
Since water is the substance of dielectric constant maximum on skin, when skin moisture content changes, the capacitance of skin also occurs
Variation, it is possible to by measuring skin pricktest capacitance, analyzing skin surface moisture content.Common instrument is Corneometer
CM825.The variation of water content of stratum corneum is weighed using the variation of keratoderma capacitance before and after product by measuring, so as to
Quantification is carried out to the moisture of keratoderma to evaluate such method of the moisture-keeping efficacy of cosmetics, can delicately be reacted
The variation of skin moisture content, and favorable reproducibility are one of current common methods of moisture-keeping cosmetics efficacy assessments.
Experimental method：Side, which is bent, in candidate or so forearm marks 3 × 3cm2The square Experimental Area of size, is made with left arm
For the test zone of moisturizer, the corresponding symmetrical region of right arm is blank control, is then tested and examined with Corneometer CM825
The moisture of each experiment position is surveyed, is repeated 5 times respectively, obtains average value.With Formulas I calculated hydration rate.
Hydration rate=(test value-blank value)/blank value × 100%
(Formulas I).
The testing result of moistening effect, as shown in table 2：
Table 2
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Reference examples 1 | Reference examples 2 | Reference examples 3 | |
| 1h | 79% | 81% | 73% | 78% | 79% | 66% | 62% | 67% |
| 2h | 65% | 60% | 66% | 59% | 63% | 52% | 47% | 55% |
| 4h | 47% | 51% | 49% | 47% | 48% | 46% | 43% | 48% |
| 6h | 43% | 46% | 43% | 42% | 44% | 43% | 40% | 43% |
| 8h | 39% | 41% | 38% | 40% | 40% | 39% | 38% | 40% |
Application Example
(1) facial cleanser formula is (by mass percentage) as shown in table 3：
Table 3
Facial cleanser manufacture craft is as follows：
1. by sodium sulfate of polyethenoxy ether of fatty alcohol, lauryl sodium sulfate, coco-nut oil fatty acid diethanol acyl in A phases
Amine, Cocoamidopropyl betaine, dodecyldimethyl ammonium oxide, glycerine and methylisothiazolinone are dissolved in deionization
Water is heated to 70 DEG C while stirring；
2. it is heated to 70 DEG C while stirring after lanolin in B phases and stearic acid are mixed；
3. by step, 2. gains are slowly added to step 1. in gains, and 65 DEG C of stirring homogeneous cool to 50 DEG C, will be in C phases
Saccharomycetes to make fermentation compound add in, continue to stir；
4. stopping stirring after temperature is down to 35 DEG C, discharge to get saccharomycetes to make fermentation compound facial cleanser.
(2) toner formula is (by mass percentage) as shown in table 4：
Table 4
Toner manufacture craft is as follows：
It is 1. 1,3 butylene glycol, methylisothiazolinone, dipotassium glycyrrhizinate, Sodium Hyaluronate, saccharomycetes to make fermentation in B phases is multiple
Square composition and deionized water mixing are stirred to dissolving；
2. glycerine, leukotrienes, Tween-80, Retinol Palmitate and Vitwas E in A phases are stirred equal
3. by step, 2. gains are added to step 1. in gains, stir evenly, filter to obtain the final product.
(3) preparation of skin whitening, moisturizing frost
The present invention takes above-mentioned saccharomycetes to make fermentation compound to be configured to skin whitening, moisturizing frost, and formula (presses quality as shown in table 5
Percentages)：
Table 5
The manufacture craft of skin whitening, moisturizing frost is as follows：
1. the caprylic/capric triglyceride in A phases, jojoba oil, Butyrospermum parkii fruit fat, ring poly- two are added in oil phase pot
Methylsiloxane, stearine, cetostearyl alcohol, PEG-20 methyl semi-solid fat acid esters and methyl semihard resin acid
Ester is heated to 85 DEG C while stirring；
2. adding in each ingredient in B phases in water phase pot, it is heated to 85 DEG C while stirring；
3. oil pumping mutually and in water phase to emulsification pot stirs homogeneous respectively after being vacuumized to emulsification pot；
4. adding in C phases triethanolamine homogeneous again after temperature is down to 65 DEG C, it is different to be cooled to the methyl added in D phases after 45 DEG C
Thiazolinone adds in E phase saccharomycetes to make fermentation compounds under stirring；
5. stopping stirring after temperature is down to 36 DEG C, discharging obtains saccharomycetes to make fermentation compound skin whitening, moisturizing frost.
Moisturizing skin whitener is made as stated above by the saccharomycetes to make fermentation compound of Examples 1 to 5 respectively to order respectively
Entitled skin whitening, moisturizing frost 1, skin whitening, moisturizing frost 2, skin whitening, moisturizing frost 3, skin whitening, moisturizing frost 4, skin whitening, moisturizing frost 5.
The above-mentioned moisturizing skin whitener being prepared is subjected to clinic trial observation.
1. volunteer's condition：
(1) women, age (gestational period or women breast-feeding their children except) between 18~60 years old；The different degrees of pigment of face
The problems such as calm, dark yellow, dry skin 35.
(2) without serious systemic disease, without immune deficiency or autoimmune disease person, recipient site did not receive skin
Treatment, beauty and other may influence the test of result；
(3) no active anaphylactia person；
(4) without the extremely sensitive person of constitution；
(5) hormone medicine and immunosuppressor person are not used in nearly one month；
(6) present or nearest three months recipient sites do not participate in other clinical trial persons.
Observation method：Before sleep every night after cleaning skin, above-mentioned skin whitening, moisturizing frost is coated.It is one to be used continuously 35 days
A course for the treatment of.Criterion of therapeutical effect：(1) it is effective：Pigment is dark yellow to subside up to more than 90%, and skin has apparent whitening to moisten smooth feeling；(2)
Effectively：Dark yellow more than 50% recession of pigment, skin is moistened smooth compared with whitening；(3) it is invalid：It is unchanged before and after treatment.As a result such as table 6
It is shown：
Table 6
| It is effective | Effectively | In vain | Total effective rate | |
| Skin whitening, moisturizing frost 1 | 29 | 1 | 5 | 85.71% |
| Skin whitening, moisturizing frost 2 | 27 | 2 | 6 | 82.86% |
| Skin whitening, moisturizing frost 3 | 28 | 3 | 4 | 88.57% |
| Skin whitening, moisturizing frost 4 | 27 | 4 | 4 | 88.57% |
| Skin whitening, moisturizing frost 5 | 28 | 4 | 3 | 91.43% |
There is no the allergic symptoms such as irritation or itch, erythema to feed back or describe in 35 (every groups) under observation.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of saccharomycetes to make fermentation compound, it is characterised in that：It is that Rhizoma Polygonati Odorati extract and jasmine flower extract are added in into ferment
In female culture medium, the zymotic fluid that is obtained after being fermented with saccharomycete.
2. saccharomycetes to make fermentation compound according to claim 1, it is characterised in that：
The Rhizoma Polygonati Odorati extract is water extract；
The jasmine flower extract is water extract；
The yeast culture medium is to be suitble to the culture medium of Yeast Growth breeding；
The condition of the fermentation is to be suitble to the condition of Yeast Growth breeding.
3. saccharomycetes to make fermentation compound according to claim 2, it is characterised in that：
The Rhizoma Polygonati Odorati extract is is made by the steps to obtain：Fragrant Solomonseal Rhizome and water are mixed, ultrasonic extraction, Ran Houjia
Heat boils extraction, and concentration obtains Rhizoma Polygonati Odorati extract；
The jasmine flower extract is is made by the steps to obtain：Dried jasmine flower and water are mixed, heating infiltration, mistake
Filter, obtains jasmine flower extract.
4. saccharomycetes to make fermentation compound according to claim 1, it is characterised in that：
The Rhizoma Polygonati Odorati extract and the jasmine flower extract in mass ratio 0.01:1~100：1 proportioning；
The saccharomycete must can Candida (Candida lambica) CGMCC 2.1763 and saccharomyces cerevisiae for youth
It is one or two kinds of in (Saccharomyces cerevisiae) CGMCC 2.1；
The condition of the fermentation is as follows：PH value is 4.0~7.0, and mixing speed is 60~250rpm, and ventilatory capacity is 1~4VVM,
Fermentation temperature is 25 DEG C~30 DEG C, and fermentation time is 2 days~4 days.
5. the preparation method of Claims 1 to 4 any one of them saccharomycetes to make fermentation compound, it is characterised in that comprising such as
Lower step：
(1) Fragrant Solomonseal Rhizome and water are mixed, ultrasonic extraction, extraction is boiled in then heating, is concentrated, is obtained Rhizoma Polygonati Odorati extract；
(2) dried jasmine flower and water are mixed, heating infiltration, filtering obtains jasmine flower extract；
(3) above-mentioned Rhizoma Polygonati Odorati extract with jasmine flower extract is mixed, stirred evenly, filtered, degerming obtains mixture；It will be mixed
It closes object to mix with by the yeast culture medium of bacteria removing, obtains fermentation medium；
(4) saccharomycete is inoculated into fermentation medium and fermented；
(5) the Preliminary fermentation liquid obtained after fermentation is subjected to separation of solid and liquid, takes liquid, obtain saccharomycetes to make fermentation compound.
6. the preparation method of saccharomycetes to make fermentation compound according to claim 5, it is characterised in that：
The condition of ultrasonic extraction described in step (1) is in 50~60 DEG C, 100~500W ultrasonic extractions 30~60 minutes；
The time that extraction is boiled in heating described in step (1) is 30~90 minutes；
The degree of concentration described in step (1) is is concentrated to density 1.01~1.20；
The dosage of water described in step (2) is is equivalent to 5~10 times of dried jasmine flower quality；
The condition of heating infiltration described in step (2) is to infiltrate 0.5~4h in 70 DEG C~100 DEG C；
Rhizoma Polygonati Odorati extract and the jasmine flower extract in mass ratio 0.01 described in step (3):1~100：1 proportioning；
The addition volume of mixture described in step (3) is the 10~20% of the yeast culture medium volume.
7. the preparation method of saccharomycetes to make fermentation compound according to claim 6, it is characterised in that：
The power of described ultrasound is in 50~60 DEG C, 100~300W ultrasonic extractions 30~60 minutes；
The condition of the heating infiltration is to infiltrate 2~4h in 70 DEG C~90 DEG C；
The Rhizoma Polygonati Odorati extract and the jasmine flower extract in mass ratio 1：1~20：1 proportioning；
The yeast culture medium is YPDA culture mediums.
8. Claims 1 to 4 any one of them saccharomycetes to make fermentation compound answering in skin whitening, moisturizing skin care item are prepared
With.
9. application of the saccharomycetes to make fermentation compound according to claim 8 in skin whitening, moisturizing skin care item are prepared,
It is characterized in that：A concentration of mass percent 1 of the saccharomycetes to make fermentation compound in the skin whitening, moisturizing skin care item
~30%.
10. a kind of skin whitening, moisturizing skin care item, it is characterised in that：It is answered containing Claims 1 to 4 any one of them saccharomycetes to make fermentation
Square composition.
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| CN106860233A (en) * | 2017-01-18 | 2017-06-20 | 长沙协浩吉生物工程有限公司 | A kind of compound method of ferment antiallergy emulsion |
| CN107823026A (en) * | 2017-12-24 | 2018-03-23 | 陈霞 | A kind of fermentation and its is preparing the application of skin whitening, moisturizing skin care item at compound |
| CN109350584B (en) * | 2018-10-17 | 2022-12-13 | 广州市孝真生物科技有限公司 | Fermented raw juice of traditional Chinese medicine composition with powerful moisturizing effect and preparation and application thereof |
| CN110680774B (en) * | 2019-10-28 | 2022-07-01 | 上海仪玳化妆品有限公司 | Preparation method of jasmine double-bacterium fermented cosmetic and application of product |
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| CN114099407A (en) * | 2021-09-09 | 2022-03-01 | 四川省宜宾高洲酒业有限责任公司 | Preparation method of natural multi-effect skin lotion |
| CN114621881A (en) * | 2021-12-30 | 2022-06-14 | 广州君研生物科技有限公司 | Saccharomyces cerevisiae fermentation liquor and skin care product comprising saccharomyces cerevisiae fermentation liquor |
| CN115554226B (en) * | 2022-11-11 | 2023-03-31 | 广州优科生物科技有限公司 | Multi-strain fermentation filtrate and preparation method and application thereof |
| CN115737454A (en) * | 2022-11-30 | 2023-03-07 | 何直源 | Double-bacterium composite extract and preparation method and application thereof |
| CN116035994B (en) * | 2023-02-08 | 2024-08-20 | 上海侬本雅生物科技有限公司 | Method for preparing polygonatum odoratum extract by adopting probiotics fermentation |
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