CN105418787B - Extraction method of polysaccharides in a kind of moringa seeds - Google Patents
Extraction method of polysaccharides in a kind of moringa seeds Download PDFInfo
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- CN105418787B CN105418787B CN201511008885.0A CN201511008885A CN105418787B CN 105418787 B CN105418787 B CN 105418787B CN 201511008885 A CN201511008885 A CN 201511008885A CN 105418787 B CN105418787 B CN 105418787B
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- moringa seeds
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Extraction method of polysaccharides in a kind of moringa seeds, it is related to extraction method of polysaccharides in a kind of moringa seeds.The low yield that polysaccharide in moringa seeds polysaccharide extraction liquid be present the invention aims to solve the problems, such as the extracting method of polysaccharide in existing moringa seeds.Extraction method of polysaccharides in moringa seeds：Powdered moringa seeds are put into water, extract certain time at a certain temperature, obtain moringa seeds polysaccharide extraction liquid；The yield of polysaccharide reaches more than 14% in described moringa seeds polysaccharide extraction liquid.Advantage：The quality of strict control moringa seeds of the invention and the volume of water when extract problem and time, the yield of polysaccharide in moringa seeds polysaccharide extraction liquid is reached more than 14%, and reduce the consumption of water resource.Present invention is mainly used for extract polysaccharide in moringa seeds.
Description
Technical field
The present invention relates to extraction method of polysaccharides in a kind of moringa seeds.
Background technology
Moringa is a kind of tropical plants being worth with abundant nutrition and unique economic, is popular in the international market；
According to Thailand of India Jerry Millner all College of Horticultures of agriculture university, Moringa root crust because unusable containing two kinds of alkaloids,
In addition, the equal edible of Moringa complete stool, its taste micro-pungent, tepor and nontoxic.Moringa is not only the preferable food of vegetarian, more
It is the natural nutrition storehouse for solving the problems, such as poor regional nutritional deficiency.With anti-inflammatory, analgesic, decompression, protect liver, cardiac stimulant, diuresis, lactagogue
And other effects, because of as many as its effect, through commonly using its pharmacy, with prevent and treat digestive system neoplasm, immunity underground, hypertension,
The diseases such as scurvy, anaemia, skin disease, diabetes, obesity.
The extracting method of plant polyose is generally extracting in boiling water, and cycle length, temperature is high, and liquid ratio is big, is unfavorable for industrializing
Production.The extracting method of polysaccharide is in current existing moringa seeds：The liquid ratio of moringa seeds and water is：30-80mL/g；Extraction temperature
Spend for 100 DEG C, extraction time is 1-4 hours, and the yield for obtaining polysaccharide in moringa seeds polysaccharide extraction liquid is only up to 9.83%.
The content of the invention
The invention aims to solve the extracting method of polysaccharide in existing moringa seeds to exist in moringa seeds polysaccharide extraction liquid
The problem of low yield of polysaccharide；And provide extraction method of polysaccharides in a kind of moringa seeds.
Extraction method of polysaccharides in a kind of moringa seeds, is specifically realized by the following steps：Powdered moringa seeds are put into water
In, 1h~1.10h is extracted at being 78~79 DEG C in temperature, obtains moringa seeds polysaccharide extraction liquid；Described moringa seeds polysaccharide extraction liquid
The yield of middle polysaccharide reaches more than 14%；The quality of described powdered moringa seeds and the volume ratio of water are 1g:(55mL~
56mL)。
Advantage of the present invention：The quality of strict control moringa seeds of the invention and the volume of water when extract problem and time, make peppery
The yield of polysaccharide reaches more than 14% in wooden seed polysaccharide extract solution, and reduces the consumption of water resource.
Embodiment
Embodiment one：Present embodiment is extraction method of polysaccharides in a kind of moringa seeds, specifically according to the following steps
Complete：Powdered moringa seeds are put into water, 1h~1.10h is extracted at being 78~79 DEG C in temperature, obtains Moringa seed polysaccharide and carry
Take liquid；The yield of polysaccharide reaches more than 14% in described moringa seeds polysaccharide extraction liquid；The quality of described powdered moringa seeds
Volume ratio with water is 1g:(55mL~56mL).
The extracting method of plant polyose is generally extracting in boiling water, thus in existing moringa seeds the extracting method of polysaccharide extraction
Temperature is 100 DEG C, and the general time for assert extraction, the longer the better, thus in existing moringa seeds the extracting method of polysaccharide time
For up to arrive 4h；Present embodiment jumps out existing thinking, with extraction method of polysaccharides in moringa seeds be related to the quality of moringa seeds with
The volume ratio of water is (referred to as：Liquid ratio), Extracting temperature and extraction time as three changed factors, utilize Design Expert
Software optimizes according to Box-Behnken design principles, and Box-Behnken (BBD) center combination design model is in response to face
Analytic approach, with three changed factors, only with three levels, compared with Orthogonal Method, obtained a result with a small amount of experimental group can,
And resulting optimum condition is not the value of setting but within the scope of imposing a condition.By taking response phase method to analyze
Moringa seeds Polyose extraction is most had ready conditions, by extraction time, extraction temperature and solid-liquid ratio to moringa seeds polysaccharide extract rate
Influence factor optimize, obtain optimal optimal conditions.
Embodiment two：Present embodiment is with the difference of embodiment one：Described powdered moringa seeds
Prepare according to the following steps：Moringa seeds first pass around degreasing, de- albumen and depigmentation processing, then successively by drying and grinding
Mill, that is, obtain powdered moringa seeds.Other are identical with embodiment one.
Embodiment three：Present embodiment is with one of embodiment one or two difference：Described powder
Shape moringa seeds moisture content≤8%.Other are identical with embodiment one or two.
Embodiment four：Present embodiment is with one of embodiment one to three difference：It is in temperature
1h~1.10h is extracted at 78.5 DEG C.Other are identical with embodiment one to three.
Embodiment five：Present embodiment is with one of embodiment one to four difference：It is 78 in temperature
1.08h is extracted at~79 DEG C.Other are identical with embodiment one to four.
Embodiment six：Present embodiment is with one of embodiment one to five difference：Described powder
The quality of shape moringa seeds and the volume ratio of water are 1g:55.6mL.Other are identical with embodiment one to five.
Embodiment seven：Present embodiment is with one of embodiment one to six difference：Moringa seeds are done
It is dry then to crush to moisture content≤8%, 80 mesh sieves are crossed, powdered moringa seeds is obtained, powdered moringa seeds is put into water,
Temperature is to extract 1.08h at 78.5 DEG C, obtains moringa seeds polysaccharide extraction liquid；The yield of polysaccharide in described moringa seeds polysaccharide extraction liquid
For 14.31%；The quality of described moringa seeds and the volume ratio of water are 1g:55.6mL.Other and embodiment one to six
It is identical.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several embodiments
Contract sample can also realize the purpose of invention.
Using following verification experimental verifications effect of the present invention
Experiment 1, a kind of moringa seeds extraction method of polysaccharides, comprise the following steps：
By moringa seeds drying to moisture content≤8%, then crush, cross 80 mesh sieves, powdered moringa seeds are obtained, by 1g powder
Shape moringa seeds are put into 55.6mL deionized waters, are extracted 1.08h at being 78.5 DEG C in temperature, are obtained moringa seeds polysaccharide extraction liquid.
20min, supernatant are centrifuged to moringa seeds polysaccharide extraction liquid in the case where temperature is 15 DEG C and rotating speed is 4000rpm
Liquid reclaims, and with deionization constant volume, obtains determinand, with the content of polysaccharide in the phend-sulphuric acid quantitative determination determinand of improvement.
The rate of output is calculated with formula：Yield (%)=[extract concentrations × constant volume/raw material dry weight] × 100%；Can by calculating
The yield for knowing polysaccharide in moringa seeds polysaccharide extraction liquid prepared by experiment 1 is 14.31%.
The phend-sulphuric acid of improvement is from open source literature " the phend-sulphuric acid measure polysaccharide and oligosaccharide content of improvement
Research "；《Chinese Pharmaceutical Journal》, 1996 (9):550-553.
Experiment 2, extraction time change the influence to polysaccharide yield
By moringa seeds drying to moisture content≤8%, then crush, cross 80 mesh sieves, powdered moringa seeds are obtained, by 1g powder
Shape moringa seeds are put into 55.6mL deionized waters, are extracted 2h at being 78.5 DEG C in temperature, are obtained moringa seeds polysaccharide extraction liquid.
The yield that polysaccharide in the moringa seeds polysaccharide extraction liquid of the preparation of experiment 2 is understood by calculating is 13.21%.
Experiment 3, Extracting temperature change the influence to polysaccharide yield：
By moringa seeds drying to moisture content≤8%, then crush, cross 80 mesh sieves, powdered moringa seeds are obtained, by 1g powder
Shape moringa seeds are put into 55.6mL deionized waters, are extracted 1.08h at being 70 DEG C in temperature, are obtained moringa seeds polysaccharide extraction liquid.
The yield that polysaccharide in the moringa seeds polysaccharide extraction liquid of the preparation of experiment 3 is understood by calculating is 13.89%.
Experiment 4, extraction liquid ratio change the influence to polysaccharide yield
By moringa seeds drying to moisture content≤8%, then crush, cross 80 mesh sieves, powdered moringa seeds are obtained, by 1g powder
Shape moringa seeds are put into 40mL deionized waters, are extracted 1.08h at being 78.5 DEG C in temperature, are obtained moringa seeds polysaccharide extraction liquid.
The yield that polysaccharide in the moringa seeds polysaccharide extraction liquid of the preparation of experiment 4 is understood by calculating is 10.24%.
Claims (1)
1. extraction method of polysaccharides in a kind of moringa seeds, it is characterised in that it is completed according to the following steps：By moringa seeds dry to
Moisture content≤8%, then crush, cross 80 mesh sieves, obtain powdered moringa seeds, powdered moringa seeds are put into water, in temperature
To extract 1.08h at 78.5 DEG C, moringa seeds polysaccharide extraction liquid is obtained；The yield of polysaccharide is in described moringa seeds polysaccharide extraction liquid
14.31%；The quality of described moringa seeds and the volume ratio of water are 1g:55.6mL.
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| CN201511008885.0A CN105418787B (en) | 2015-12-28 | 2015-12-28 | Extraction method of polysaccharides in a kind of moringa seeds |
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| CN201511008885.0A CN105418787B (en) | 2015-12-28 | 2015-12-28 | Extraction method of polysaccharides in a kind of moringa seeds |
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| CN105418787A CN105418787A (en) | 2016-03-23 |
| CN105418787B true CN105418787B (en) | 2018-03-27 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108752489A (en) * | 2018-05-08 | 2018-11-06 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and ethylene glycol |
| CN108676102A (en) * | 2018-05-08 | 2018-10-19 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and glycerine |
| CN108373512A (en) * | 2018-05-08 | 2018-08-07 | 桂林理工大学 | A method of the depth congruent melting solvent extraction Moringa seed polysaccharide made of choline chloride and urea |
| CN108771074B (en) * | 2018-05-17 | 2021-08-31 | 广西贺州市桑辰科技有限公司 | Preparation method of moringa seed solid beverage |
| CN110003352A (en) * | 2019-04-12 | 2019-07-12 | 北部湾大学 | A kind of extracting method that moringa seeds selenium active constituent is effectively kept |
| CN110721134A (en) * | 2019-11-29 | 2020-01-24 | 广州环亚化妆品科技有限公司 | Method for extracting tyrosinase inhibitor from moringa seeds, tyrosinase inhibitor prepared by same and application thereof |
| CN113024679B (en) * | 2021-02-25 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for extracting selenium polysaccharide and polyphenol from selenium-rich moringa seeds |
| CN115521384A (en) * | 2022-09-28 | 2022-12-27 | 无锡绿色分离应用技术研究所有限公司 | High-efficiency extraction method of moringa oleifera polysaccharide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102617747A (en) * | 2012-03-26 | 2012-08-01 | 浙江大学 | Extracting process of dendrobium candidum polysaccharides and method for optimizing process by response surface method |
| CN104829743A (en) * | 2015-05-25 | 2015-08-12 | 萧丽雅 | Preparation method and use of moringa oleifera leaf polysaccharides |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102617747A (en) * | 2012-03-26 | 2012-08-01 | 浙江大学 | Extracting process of dendrobium candidum polysaccharides and method for optimizing process by response surface method |
| CN104829743A (en) * | 2015-05-25 | 2015-08-12 | 萧丽雅 | Preparation method and use of moringa oleifera leaf polysaccharides |
Non-Patent Citations (1)
| Title |
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| 辣木水溶性多糖提取工艺的研究;任安祥 等;《食品科技》;20080531(第5期);第1.3-1.4节 * |
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