CN105325274A - Paphiopedilum seed germination culture medium, culture method and application - Google Patents
Paphiopedilum seed germination culture medium, culture method and application Download PDFInfo
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- CN105325274A CN105325274A CN201510732333.8A CN201510732333A CN105325274A CN 105325274 A CN105325274 A CN 105325274A CN 201510732333 A CN201510732333 A CN 201510732333A CN 105325274 A CN105325274 A CN 105325274A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the field of plant seedling propagation, and in particular relates to a paphiopedilum seed germination culture medium, a culture method and application. The paphiopedilum seed germination culture medium adopts a 1/4 MS culture medium as a basal culture medium, and comprises the following components with the content: 100mL/L of pure coconut juice, 1g/L of activated carbon, 0.5g/L of peptone, and 1-16mg/L of diethylaminoethyl hexanoate. The culture method comprises the following steps of grafting paphiopedilum seeds into the paphiopedilum seed germination culture medium, and culturing at the temperature of 22 DEG C and under the condition of darkness until seed germination. According to the paphiopedilum seed germination culture medium and the culture method thereof, the germination rate is greatly improved, and the production efficiency is improved.
Description
Technical field
The invention belongs to plant seedling breeding field, be specifically related to the blue seed germination medium of a kind of pocket and cultural method and application.
Background technology
The general designation that pocket orchid (PaphiopedilumPfitzer) is all plants of the orchid family Paphiopedilum, be also called celestial carry out blue, slippers are blue, belong to the monoid that in the orchid family (Orchidaceae) one is comparatively original, full genus about has 76 kinds, mainly be distributed in subspecies torrid areas to Pacific Islands, growth is under tropical rain forest, and ground is raw or grow nonparasitically upon another plant.Wherein paphiopedilum armeniacum (P.armeniacum) and spend pocket orchid (P.emersonii) in vain for China endemic species.The rare and endangered species of I level that full genus is all listed in " wild animals and plants Convention on International Trade in Endangered Species (CITES) " protection, simultaneously Ye Shi China first class of protection rare plant.(Luo Yibo etc., 2003)
Pocket orchid type is unique, and plant type is graceful, beautiful in colour, and the florescence is long, is one of orchid kind most popular in global range.Cultivation and the Breeding history of pocket orchid are long, are developed so far the Hybrid having enormous amount, have fans the most numerous in worldwide.Traditional modes of reproduction of pocket orchid is division propagation, and reproduction speed is extremely slow, though the tissue culture technology utilizing explant to increase has some to report, can't carry out industrialization operation.The in vitro non-symbiosis germination technology of seed is the blue seeling industry technology of pocket generally adopted in current global range.The blue seed of pocket is small, and not containing endosperm, under natural conditions, it is sprouted needs and mycosymbiosis, and germination rate is extremely low.The problems such as though early have research for the in vitro non-symbiosis germination technology of Paphiopedilum, it is low that ubiquity sprouts quantity, and sprouting state is unstable; Its vegetative propagation, namely clones very difficult especially; Therefore commercially producing of pocket orchid obtains fruit mainly through artificial pollination, and the mode that the in vitro non-symbiosis germination carrying out seed obtains seedling obtains seedling; Be limited to the low and poor stability of the germination rate of seed, large-scale production has certain difficulty.
Summary of the invention
In order to overcome the deficiencies in the prior art and shortcoming, primary and foremost purpose of the present invention is to provide a kind of pocket blue seed germination medium.
Another object of the present invention is to provide a kind of cultural method improving the in vitro non-symbiosis germination rate of the blue seed of pocket, this cultural method effectively can improve the germination rate of the in vitro non-symbiosis germination of the blue seed of pocket, effectively shortens and sprouts the time used.
Another object of the present invention is the application providing the blue seed germination medium of above-mentioned pocket.
Object of the present invention is achieved by the following technical programs:
The blue seed germination medium of a kind of pocket, medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
The content of described diethyl aminoethyl hexanoate is preferably 1 ~ 8 mg/litre;
Content more preferably 8 mg/litre of described diethyl aminoethyl hexanoate;
Improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, comprise following steps:
By in the blue seed germination medium of blue for pocket seed access pocket, 22 DEG C, be cultured to seed germination under dark condition;
The blue seed of described pocket is preferably the blue seed of red magic Supreme Being's pocket;
The blue seed of described pocket is preferably by such as under type acquisition: select the blue fruit of the pocket of latter 180 days of pollination (180DAP), after surface sterilization, cut fruit, the blue seed of acquisition pocket;
The mercuric chloride solution that the mode of described surface sterilization is preferably employing 0.1% (w/v) soaks 7 ~ 15 minutes, and period repeatedly shakes, to ensure the effect of surface sterilization;
The time of described cultivation is preferably 50 days;
Described accesses concrete being operating as of the blue seed germination medium of pocket by blue for pocket seed: by blue for pocket seed broadcasting on the blue seed germination medium surface of sterilized pocket;
Described diethyl aminoethyl hexanoate chemical name is acetic acid Diethylaminoethanol fat (DiethylAminoethylHexanoate), molecular formula C
12h
25nO
2, molecular weight 215.33, CAS is numbered 10369-83-2;
The application of the blue seed germination medium of described pocket in the blue breeding field of pocket;
The present invention has following advantage and effect relative to prior art:
(1) the blue seed germination medium composition of pocket provided by the invention is simple, cost is low, adopt medium based on 1/4MS medium, by reducing the ion concentration of macroelement, being conducive to the blue seed germination of pocket, improving the in vitro non-symbiosis germination rate of the blue seed of pocket.
(2) the present invention based on 1/4MS medium on, the blue seed germination rate of pocket can be significantly improved by adding appropriate diethyl aminoethyl hexanoate and shorten sprout time, wherein germination rate is up to more than 90%, adds the sprouting that too high or too low diethyl aminoethyl hexanoate is all unfavorable for the blue seed of pocket.
(3) the present invention based on 1/4MS medium on, by adding active carbon, peptone, diethyl aminoethyl hexanoate cooperate with seed, phenols, quinones substance that effective absorbed seed produces, Browning control, break the dormancy of seed, promote seed germination, significantly shorten sprout time.
(4) the invention provides a kind of cultural method improving the in vitro non-symbiosis germination rate of the blue seed of pocket, the method have easy and simple to handle, cost is low, improve the effect of the germination rate of the in vitro non-symbiosis germination of seed, the large-scale breeding that may be used for pocket orchid is produced.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
The blue seed germination medium of a kind of pocket, medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
Improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, comprise the following steps:
(1) acquisition of explant: when the blue plant blossom of red magic Supreme Being's pocket, choose the good plant of growing way and carry out artificial pollination as parent, latter 180 days picking fruits of pollinating, seed is as explant material in fruit, carries out aseptic seeding;
(2) mercuric chloride solution that the fruit after step (1) being gathered is placed in 0.10% (w/v) carries out disinfection 12 minutes, and period repeatedly shakes, to ensure the effect of surface sterilization; Then rinsed with sterile water is used 3 times; In superclean bench, cut fruit, seed is sprinkling upon the blue seed germination medium surface of sterilized pocket;
(3) postvaccinal blake bottle is placed in 22 DEG C, cultivates 50 days under dark condition, when after seed germination, be cultured to 2 true leaves under proceeding to the illumination condition of 2000lux and formed, namely switchable;
The germination rate of the blue seed of the present embodiment pocket is 27.2%.
Embodiment 2
The blue seed germination medium of a kind of pocket, medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
Improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, comprise the following steps:
(1) acquisition of explant: when the blue plant blossom of red magic Supreme Being's pocket, choose the good plant of growing way and carry out artificial pollination as parent, latter 180 days picking fruits of pollinating, seed is as explant material in fruit, carries out aseptic seeding;
(2) mercuric chloride solution that the fruit after step (1) being gathered is placed in 0.10% (w/v) carries out disinfection 12 minutes, and period repeatedly shakes, to ensure the effect of surface sterilization; Then rinsed with sterile water is used 3 times; In superclean bench, cut fruit, seed is sprinkling upon the blue seed germination medium surface of sterilized pocket.
(3) postvaccinal blake bottle is placed in 22 DEG C, cultivates 50 days under dark condition, when after seed germination, be cultured to 2 true leaves under proceeding to the illumination condition of 2000lux and formed, namely switchable;
The germination rate of the blue seed of the present embodiment pocket is 90.1%.
Embodiment 3
The blue seed germination medium of a kind of pocket, medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
Improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, comprise the following steps:
(1) acquisition of explant: when the blue plant blossom of red magic Supreme Being's pocket, choose the good plant of growing way and carry out artificial pollination as parent, latter 180 days picking fruits of pollinating, seed is as material in fruit, carries out aseptic seeding;
(2) mercuric chloride solution that the fruit after step (1) being gathered is placed in 0.10% (w/v) carries out disinfection 12 minutes, and period repeatedly shakes, to ensure the effect of surface sterilization; Then rinsed with sterile water is used 3 times; In superclean bench, cut fruit, seed is sprinkling upon the blue seed germination medium surface of sterilized pocket.
(3) postvaccinal blake bottle is placed in 22 DEG C, cultivates 50 days under dark condition, when after seed germination, be cultured to 2 true leaves under proceeding to the illumination condition of 2000lux and formed, namely switchable;
The germination rate of the blue seed of the present embodiment pocket is 4.2%.
Comparative examples
The blue seed germination medium of a kind of pocket, medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
Improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, comprise the following steps:
(1) acquisition of explant: when the blue plant blossom of red magic Supreme Being's pocket, choose the good plant of growing way and carry out artificial pollination as parent, latter 180 days picking fruits of pollinating, seed is as material in fruit, carries out aseptic seeding;
(2) mercuric chloride solution that the fruit after step (1) being gathered is placed in 0.10% (w/v) carries out disinfection 12 minutes, and period repeatedly shakes, to ensure the effect of surface sterilization; Then rinsed with sterile water is used 3 times; In superclean bench, cut fruit, seed is sprinkling upon the blue seed germination medium surface of sterilized pocket.
(3) postvaccinal blake bottle is placed in 22 DEG C, cultivates 50 days under dark condition, when after seed germination, be cultured to 2 true leaves under proceeding to the illumination condition of 2000lux and formed, namely switchable;
The germination rate of the blue seed of the present embodiment pocket is 2.6%.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. the blue seed germination medium of pocket, is characterized in that: medium based on 1/4MS medium;
The component that the blue seed germination medium of described pocket also comprises and content as follows:
2. the blue seed germination medium of pocket according to claim 1, is characterized in that:
The content of described diethyl aminoethyl hexanoate is 1 ~ 8 mg/litre.
3. the blue seed germination medium of pocket according to claim 2, is characterized in that:
The content of described diethyl aminoethyl hexanoate is 8 mg/litre.
4. improve a cultural method for the in vitro non-symbiosis germination rate of the blue seed of pocket, it is characterized in that comprising following steps:
By in the blue seed germination medium of pocket described in blue for pocket any one of seed access claim 1 ~ 3,22 DEG C, be cultured to seed germination under dark condition.
5. the cultural method of the in vitro non-symbiosis germination rate of the blue seed of raising pocket according to claim 4, is characterized in that:
The blue seed of described pocket is the blue seed of red magic Supreme Being's pocket.
6. the cultural method of the in vitro non-symbiosis germination rate of the blue seed of raising pocket according to claim 4, is characterized in that:
The blue seed of described pocket obtains in the following way: select the blue fruit of the pollination pocket of latter 180 days, after surface sterilization, cut fruit, obtains the blue seed of pocket.
7. the cultural method of the in vitro non-symbiosis germination rate of the blue seed of raising pocket according to claim 4, is characterized in that:
The mode of described surface sterilization is adopt the mercuric chloride solution of 0.1%w/v to soak 7 ~ 15 minutes.
8. the cultural method of the in vitro non-symbiosis germination rate of the blue seed of raising pocket according to claim 4, is characterized in that:
The time of described cultivation is 50 days.
9. the cultural method of the in vitro non-symbiosis germination rate of the blue seed of raising pocket according to claim 4, is characterized in that:
Described accesses concrete being operating as of the blue seed germination medium of pocket by blue for pocket seed: by blue for pocket seed broadcasting on the blue seed germination medium surface of sterilized pocket.
10. the application of the blue seed germination medium of the pocket described in claims 1 to 3 in the blue breeding field of pocket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510732333.8A CN105325274A (en) | 2015-10-30 | 2015-10-30 | Paphiopedilum seed germination culture medium, culture method and application |
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| CN201510732333.8A CN105325274A (en) | 2015-10-30 | 2015-10-30 | Paphiopedilum seed germination culture medium, culture method and application |
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| CN105325274A true CN105325274A (en) | 2016-02-17 |
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| CN201510732333.8A Pending CN105325274A (en) | 2015-10-30 | 2015-10-30 | Paphiopedilum seed germination culture medium, culture method and application |
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|---|---|---|---|---|
| CN101558744A (en) * | 2009-06-04 | 2009-10-21 | 贵州省亚热带作物研究所 | Method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds |
| CN101569285A (en) * | 2009-06-02 | 2009-11-04 | 中国科学院华南植物园 | Method for cross breeding and seedling propagation of paphiopedilum |
| CN102283114A (en) * | 2011-06-23 | 2011-12-21 | 中国科学院华南植物园 | Orchid aseptic seeding and test tube seedling breeding method and used broad-spectrum culture mediums |
| CN103416294A (en) * | 2013-08-06 | 2013-12-04 | 广西壮族自治区中国科学院广西植物研究所 | Concolor paphiopedilum crossbreeding method and seedling breeding method thereof |
| CN103704139A (en) * | 2013-12-30 | 2014-04-09 | 浙江森禾种业股份有限公司 | Multi-flower paphiopedilum test-tube seedling cultivation method |
| CN104186295A (en) * | 2014-09-29 | 2014-12-10 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for paphiopedilum seed germination and culture method |
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Patent Citations (6)
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| CN101569285A (en) * | 2009-06-02 | 2009-11-04 | 中国科学院华南植物园 | Method for cross breeding and seedling propagation of paphiopedilum |
| CN101558744A (en) * | 2009-06-04 | 2009-10-21 | 贵州省亚热带作物研究所 | Method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds |
| CN102283114A (en) * | 2011-06-23 | 2011-12-21 | 中国科学院华南植物园 | Orchid aseptic seeding and test tube seedling breeding method and used broad-spectrum culture mediums |
| CN103416294A (en) * | 2013-08-06 | 2013-12-04 | 广西壮族自治区中国科学院广西植物研究所 | Concolor paphiopedilum crossbreeding method and seedling breeding method thereof |
| CN103704139A (en) * | 2013-12-30 | 2014-04-09 | 浙江森禾种业股份有限公司 | Multi-flower paphiopedilum test-tube seedling cultivation method |
| CN104186295A (en) * | 2014-09-29 | 2014-12-10 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for paphiopedilum seed germination and culture method |
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Application publication date: 20160217 |
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