CN105301227A - Parallel reaction treatment device for any project group for immunodetection - Google Patents
Parallel reaction treatment device for any project group for immunodetection Download PDFInfo
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- CN105301227A CN105301227A CN201510740007.1A CN201510740007A CN105301227A CN 105301227 A CN105301227 A CN 105301227A CN 201510740007 A CN201510740007 A CN 201510740007A CN 105301227 A CN105301227 A CN 105301227A
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 59
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 113
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000001914 filtration Methods 0.000 claims description 29
- 239000000376 reactant Substances 0.000 claims description 24
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 6
- 230000002093 peripheral effect Effects 0.000 claims 2
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 230000037431 insertion Effects 0.000 abstract 4
- 238000003780 insertion Methods 0.000 abstract 4
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000035484 reaction time Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 14
- 238000000034 method Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 9
- 238000003018 immunoassay Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000008346 aqueous phase Substances 0.000 description 3
- 101710142246 External core antigen Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003541 multi-stage reaction Methods 0.000 description 1
- 238000011085 pressure filtration Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention discloses a parallel reaction treatment device for any project group for immunodetection. The parallel reaction treatment device comprises a turntable driven by a rotating shaft of a time delay stepping motor, wherein a plurality of fan-shaped stations are outwards arranged at the center of the turntable in a radial manner; an insertion slot for insertion of a reagent project group is formed in each fan-shaped station; a first actuator for pushing the reagent project group into each insertion slot in the lateral direction and a second actuator for pushing the reagent project group out of each insertion slot are arranged at the two radial outer ends of the turntable; a turntable region from the second actuator to the first actuator is an empty region; a turntable region from the first actuator to the second actuator is a region for parallel treatment on multiple reagent project groups; a pressurization device and a liquid adding device are arranged on the turntable at an interval. According to the parallel reaction treatment device disclosed by the invention, a plurality of index detection projects can be subjected to parallel reaction treatment in a manner of grouping, so that the reaction time interval between every two adjacent detection project groups is shortened, and the bulk sample treatment efficiency is improved; combined reaction on any project group of multiple indexes can be realized, the instrument applicable detection project range is enlarged, and the requirements on detection and diagnosis of multiple indexes and multiple disease entities are guaranteed.
Description
Technical field
The present invention relates to any project team of a kind of immune detection parallel reaction treating apparatus, specifically a kind of in-vitro diagnosis that is used for detects multi objective project team parallel reaction treating apparatus immediately.
Background technology
In instant testing process, parallel parsing and simultaneously report multi objective extremely important.Multi objective can provide more for doctor, diagnostic message more accurately, makes correct diagnosis for doctor; Multi objective parallel parsing can meet better and immediately detects needs.In actual testing process, test event, the quantity of each patient are all not identical; The project team each patient detected can synchronously must process this in groups as a unit, completes reaction, and this is extremely important for instant detection.
BioMerieuxVidas, the Mitsubishi chemistry PATHFAST of Denmark thunder degree AQT90FLEX immunity analysis instrument, French Mei Liai are the instant pick-up units of a class whole blood sample, can realize particular area entry parallel parsing and report the result.Other liquid components is distributed into the integrated morphology of single part by this kind of device in advance, adds reagent during without the need to detecting; That the test index reagent of a patient is completed test with the formal machine of group during this kind of equipment Inspection; But this quasi-instrument after this organizes index test, then need start project team's test of other patients.Project team test of each patient is generally 16 ~ 20 minutes, waits for too long, and efficiency is low, is unfavorable for that outpatient service etc. is compared with full-page proof process during multisample.
Summary of the invention
For solving the problem, the present invention aims to provide any project team of a kind of immune detection parallel reaction treating apparatus, continuous parallel processing is reacted to the multi objective immune detection project team of each patient, the stand-by period at the test item group reaction interval of multidigit patient is shortened greatly, avoids multiple project team's reaction link time interval in active immunity quantitative test oversize and affect immune detection analysis efficiency.
For reaching above-mentioned purpose, the invention provides any project team of a kind of immune detection parallel reaction treating apparatus, comprise a rotating disk driven by time delay stepping motor rotating shaft, center of turntable outwards arranges some fan-shaped stations radially, is provided with a slot of locating for the plant of Reagent Project group in each fan-shaped station; Being provided with at outside two ends, rotating disk footpath can the first actuator of Reagent Project group side direction drive slot and the second actuator releasing slot; Rotate clockwise on direction at rotating disk, the second actuator is vacant district to the rotating disk region of the first actuator; First actuator is the reaction zone of many Reagent Project group parallel processing to the rotating disk region of the second actuator; To rotate clockwise at rotating disk on the reaction zone on direction also sequentially interval arrange provide a pressue device of source of the gas to the measuring samples press filtration in Reagent Project group, provide a liquid-adding device of reactant liquor to Reagent Project group, pressue device that a pair reactant liquor press filtration provides source of the gas.
Described each Reagent Project group is made up of side by side at least one integrated Reagent Tube, and each slot two side is arranged with the arc-shaped slot compressing integrated Reagent Tube outer wall; Inwards the Reagent Project group side direction that five integrated Reagent Tubes are formed can be pushed slot along rotating disk is radial, the outer wall of each integrated Reagent Tube, by arc-shaped slot extruding location in opposite directions, can make Reagent Project group quick position in slot.
Described integrated Reagent Tube comprises one can immobilised micropore cup and one and micropore rim of a cup place's dismounting sample filtration device of joining in advance, arrange the sample filter bowl that micropore cup is inserted in a bottom in the middle of sample filtration device, sample filter bowl surrounding is connected on sample filtration device by some every beam; Sample filter bowl bottom surface is provided with the minim pipette be communicated with inside and outside sample filter bowl; Annotate in micropore cup after liquid to be measured can being filtered by sample filter bowl.
Described sample filter bowl inner bottom surface is fixed with the binding film be made up of sample filter membrane and label film-stack from top to bottom by snap ring; Determinand is obtained by reacting electrochemiluminescent immunoassay detection object reaction chain again by the micropore cup instilling solid phase in advance after binding film initial reaction.
Described pressue device comprises the vertical pole of a fixing turntable periphery, the cross bar and on a fixing vertical pole top can be fixed a point the slide block that slides along cross bar on slot, slider top is provided with a gas-tpe fitting be connected with air supply hose, arranges and communicate and the gas-adding pipe of automatically retractable with gas-tpe fitting under slide block.Time below Reagent Project group temporal persistence sheer pole, after skid to assigned address, gas-adding pipe stretches out downwards the pressure filtration of Reagent Project group.
Described cross bar is provided with the block limiting slide block sliding scale directly over slot; To guarantee that gas-adding pipe range of movement on slide block is in the length range of slot.
Described liquid-adding device comprises the vertical rod of a fixing turntable periphery, the slide bar and of a fixing rod end can be fixed a point the sliding sleeve that slides along slide bar on slot, sliding sleeve top is provided with a liquid pipe connection be connected with feed liquid hose, arranges and communicate and the liquid injection pipe of automatically retractable with liquid pipe connection under sliding sleeve.Time below Reagent Project group temporal persistence drop shot, after sliding into assigned address by sliding sleeve, liquid injection pipe can stretch out downwards and to Reagent Project group filling reactant liquor.
Described slide bar is provided with to limit and slides over the limited block of sliding scale directly over slot, to guarantee that liquid injection pipe range of movement on sliding sleeve is in the length range of slot.
After adopting technique scheme, rotating disk rotates according to the time delay order stepping of setting, the Reagent Project group of one group of group is pushed in the slot on rotating disk by the first actuator sequence side, the Reagent Project group of one group of group is after pressue device is to the press filtration of determinand, by liquid-adding device, reactant liquor is injected to the Reagent Project group of one group of group again, when the Reagent Project group of one group of group is taken out by the second actuator, make all fully to complete immune response by one group of group reagent project team of reaction zone; Make patient's test item with the parallel turnover of mode in groups and complete reaction, detecting to realize high efficiency instant electrochemiluminescent immunoassay in groups and analyze.
Beneficial effect of the present invention is as follows:
1. make the multiple determination project of each patient with the process of burst mode parallel reaction, improve the instant detection efficiency of multi objective of external electrochemiluminescent immunoassay.
2. make the reacting space time between the test item group of each patient greatly shorten, the stand-by period of patient is shortened greatly, be beneficial to compared with full-page proof process during multisample.
3. the present invention can realize the composite reaction of any project team of multi objective, greatly increases instrument and is suitable for test item scope, ensure that multi objective, many sick kinds detect, diagnosis needs.
Accompanying drawing explanation
Fig. 1 is one-piece construction vertical view of the present invention.
Fig. 2 is the structure side view of pressue device of the present invention.
Fig. 3 is pressue device principle of work schematic diagram of the present invention.
Fig. 4 is the structure side view of liquid-adding device of the present invention.
Fig. 5 is liquid-adding device principle of work schematic diagram of the present invention.
Fig. 6 is the structure side view of slot of the present invention.
Fig. 7 is the structural representation snapping in integrated Reagent Tube group (Reagent Project group) of slot of the present invention.
Fig. 8 is the structure cut-open view of the single integrated Reagent Tube of the present invention.
Fig. 9 is that an integrated Reagent Tube group is pushed the structural representation of slot location by the first actuator.
Figure 10 is integrated Reagent Tube group is sequentially pushed slot location structural representation by the first actuator.
Figure 11 is the structural representation by the air pressure of pressue device, integrated Reagent Tube group being carried out to press filtration.
Figure 12 is the structural representation carrying out adding reactant liquor to integrated Reagent Tube group by liquid-adding device.
Figure 13 is the structural representation that integrated Reagent Tube group sequentially arrives the second actuator position.
In figure, accompanying drawing is designated: 20. time delay stepping motor rotating shafts; 30. rotating disks; 40. fan-shaped stations; 50. slots; 60. first actuators; 70. second actuators; 80. reaction chamber covers; 90. pressue devices; 100. liquid-adding device; 200. integrated Reagent Tubes; 201. micropore cups; 202. filtration unit; 203. every beam; 204. sample filter bowls; 205. minim pipette; 206. snap ring; 207. binding film; 208. sample filter membranes; 209. label films; 300. vacant districts; 400. reaction zone; A. the first Reagent Project group; B. the second Reagent Project group; C. the 3rd Reagent Project group.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further described.
Any project team of a kind of immune detection parallel reaction treating apparatus as shown in Figure 1, comprise a rotating disk driven by time delay stepping motor rotating shaft 20 30, rotating disk 30 center outwards arranges some fan-shaped stations 40 radially, is provided with a slot 50 of locating for the plant of Reagent Project group in each fan-shaped station 40; Being provided with at outside two ends, rotating disk 30 1 footpath can the first actuator 60 of Reagent Project group side direction drive slot 50 and the second actuator 70 releasing slot 50; Rotate clockwise on direction at rotating disk 30, rotating disk 30 region of the second actuator 70 to the first actuator 60 is vacant district 300; Rotating disk 30 region of the first actuator 60 to the second actuator 70 is the reaction zone 400 of many Reagent Project group parallel processing; To rotate clockwise at rotating disk 30 on the reaction zone 400 on direction also sequentially interval arrange provide a pressue device 90 of source of the gas to the measuring samples press filtration in Reagent Project group, Reagent Project group provided to a liquid-adding device 100 of reactant liquor, pressue device 90 that a pair reactant liquor press filtration provides source of the gas.In the present embodiment, arranging label in reaction zone 400 is 1,2,3,4,5 ... 10,12 fan-shaped stations 40 on the corresponding rotating disk 30 in 11,12 track positions; First actuator 60 correspondence is arranged on the 1st track position in reaction zone 400, and the second actuator 70 correspondence is arranged on the 12nd track position in reaction zone 400, and two pressue devices 90 are separately positioned on the 3rd track position and the 8th track position; Liquid-adding device 100 is arranged on the 6th track position.A rotating disk 30 reaction chamber cover 80 covers; The slot 50 of vacant district 300 correspondence can be used for Reagent Project group to be measured to be inserted in slot 50 radial opening in advance, when Reagent Project group to be measured is turned to the 1st track position by rotating disk 30, is promoted to snap in slot 50 by the first actuator 60 side direction.
As shown in Figure 1, Figure 2 and Figure 3, the cross bar 92 and one that pressue device 90 of the present invention comprises fixing vertical pole 91 top of vertical pole 91, of fixing rotating disk 30 periphery can be fixed a point the slide block 93 that slides along cross bar 92 on slot 50, slide block 93 top is provided with a gas-tpe fitting 931 be connected with air supply hose 933, and slide block arranges for 93 times and communicates and the gas-adding pipe 932 of automatically retractable with gas-tpe fitting 931; Cross bar 92 is provided with the block 921 limiting slide block 93 sliding scale directly over slot 50; As shown in figures 3 and 8, slide block 93 is when cross bar 92 moves to integrated Reagent Tube 200 position, gas-adding pipe 932 stretches out downwards, the air with pressure presses down sealed sample filter bowl 204 rim of a cup by air supply hose 933 through gas-adding pipe 932, and testing sample is obtained initial reaction liquid by pressing down by instilling bottom micropore cup 201 through minim pipette 205 after binding film 206 press filtration.
As shown in Figure 1, Figure 4 and Figure 5, the slide bar 102 and one that liquid-adding device 100 of the present invention comprises fixing vertical rod 101 top of vertical rod 101, of fixing rotating disk 30 periphery can be fixed a point the sliding sleeve 103 that slides along slide bar 102 on slot 50, sliding sleeve 103 top is provided with a liquid pipe connection 1031 be connected with feed liquid hose 1033, and sliding sleeve arranges for 103 times and communicates and the liquid injection pipe 1032 of automatically retractable with liquid pipe connection 1031; Slide bar 102 is provided with the limited block 1021 limiting sliding sleeve 103 sliding scale directly over slot 50.As shown in Figure 5 and Figure 8, sliding sleeve 103 is when slide bar 102 moves to integrated Reagent Tube 200 position, liquid injection pipe 1032 stretches out downwards, reactant liquor injects sealed sample filter bowl 204 by feed liquid hose 1033 through liquid injection pipe 1032, by the initial reactant on reactant liquor press filtration washing binding film 206, instill bottom micropore cup 201 by minim pipette 205.
As shown in Fig. 1, Fig. 6 and Fig. 7, each Reagent Project group that the present invention will react is made up of side by side five integrated Reagent Tubes 200, and each slot 50 two side on rotating disk 30 is arranged with the arc-shaped slot 51 compressing integrated Reagent Tube 200 outer wall.
As shown in Figure 8, integrated Reagent Tube 200 of the present invention comprise one can in advance immobilised micropore cup 201 and one and 201 mouthfuls, micropore cup locate the sample filtration device 202 that dismounting joins, arrange the sample filter bowl 204 that micropore cup 201 is inserted in a bottom in the middle of sample filtration device 202, sample filter bowl 204 surrounding is connected on sample filtration device 202 by some every beam 203; Sample filter bowl 204 bottom surface is provided with the minim pipette 205 be communicated with inside and outside sample filter bowl 204; Sample filter bowl 204 inner bottom surface is fixed with from top to bottom by sample filter membrane 208 and the stacked binding film 207 formed of label film 209 by snap ring 206.The present invention, in advance the micropore vestibule immobilization of micropore cup 201, makes it mate with the label film 209 in sample filter bowl 204; The principle of work of the integrated Reagent Tube 200 of the present invention is: the determinand sample adding designated volume in sample filter bowl 204, cell granulations in determinand is intercepted in film upper strata by sample filter membrane 208, aqueous phase oozes to label film 209 downwards from sample filter membrane 208 hole, label in label film 209 by aqueous phase dissolved and with the initial association reaction in label film 209 of the determinand in aqueous phase, obtain initial reactant; The initial reactant in sample filter bowl 204 is made to flow into the solid formation association reaction on micropore cup 201 inwall by minim pipette 205 by gas positive pressure, even if after the acellular composition in initial reactant and the label solid phase region entered in micropore cup 201 participates in reaction; Form solid phase-testing molecule-label reaction chain, for follow-up, electrochemiluminescent immunoassay detection is carried out to object.
The following detailed description of principle of work of the present invention with specifically implement action.
Process as shown in Fig. 9 ~ Figure 13 is that the reactions steps of case 1 is as follows:
Load case 1: being designated location in the first Reagent Project group A that the integrated Reagent Tube 200 of TSH, T3, FT3, T4, FT4 five forms to block on the rotating disk 30 advancing corresponding 1st track position slot 50 by the first actuator 60.
First Reagent Project group A is detained reaction minute 1 minute respectively in the 1st, 2 track positions.
First Reagent Project group A in the 3rd track position by pressue device 90 malleation mode respectively by sample press filtration in integrated for TSH, T3, FT3, T4, FT4 five Reagent Tube 200 to the micropore cup 201 inner bottom part reaction zone of integrated Reagent Tube 200.
First Reagent Project group A moves to the 4th, 5 track positions, each delay 1 minute.
First Reagent Project group A adds reactant liquor by liquid-adding device 100 in each integrated Reagent Tube 200 when the 6th track position.
Reaction zone bottom the initial reactant press filtration that reactant liquor washs in the 8th track position by the first Reagent Project group A by each integrated Reagent Tube 200 to micropore cup 201.
Each integrated Reagent Tube 200 top filtration unit 202 takes out in the 9th, 10,11 any track positions and abandons by the first Reagent Project group A.
Period: the first Reagent Project group A moves to the 9th, 10,11,12 track positions and is respectively detained 1 minute.
First Reagent Project group A is after the 12nd track steric retardation stays end in 1 minute, and the first Reagent Project group A entirety is released slot 50 by the second actuator 70 and entered washing link.
Process as shown in Figure 10 ~ Figure 13 is that the reactions steps of case 2 is as follows:
After TSH, T3, FT3, T4, FT4 five integrated project group cards of case 1 advance reaction unit the 2nd track positions, on rotating disk 30, in the slot 50 of the 1st track position correspondence, load case 2 Reagent Project group at once.
Load case 2: being designated location in the second Reagent Project group B that the integrated Reagent Tube 200 of TSH, FT3, FT4 tri-forms to block on the rotating disk 30 advancing corresponding 1st track position slot 50 by the first actuator 60.
Second Reagent Project group B is detained reaction minute 1 minute respectively in the 1st, 2 track positions.
Second Reagent Project group B in the 3rd track position by pressue device 90 malleation mode respectively by sample press filtration in integrated for TSH, FT3, FT4 tri-Reagent Tube 200 to the micropore cup 201 inner bottom part reaction zone of integrated Reagent Tube 200.
Second Reagent Project group B moves to the 4th, 5 track positions, each delay 1 minute.
Second Reagent Project group B adds reactant liquor by liquid-adding device 100 in each integrated Reagent Tube 200 when the 6th track position.
Reaction zone bottom the initial reactant press filtration that reactant liquor washs in the 8th track position by the second Reagent Project group B by each integrated Reagent Tube 200 to micropore cup 201.
Each integrated Reagent Tube 200 top filtration unit 202 takes out in the 9th, 10,11 any track positions and abandons by the second Reagent Project group B.
Period: the second Reagent Project group B moves to the 9th, 10,11,12 track positions and is respectively detained 1 minute.
Second Reagent Project group B is after the 12nd track steric retardation stays end in 1 minute, and the second Reagent Project group B entirety is released slot 50 by the second actuator 70 and entered washing link.
Process as shown in Figure 11 ~ Figure 13 is that the reactions steps of case 3 is as follows:
After TSH, FT3, FT4 tri-integrated project group cards of case 2 advance reaction unit the 2nd track positions, on rotating disk 30, in the slot 50 of the 1st track position correspondence, load case 3 Reagent Project group at once.
Load case 3: being designated HBsAg, Anti-HBsAg antibody, HBeAg, anti-HBe, the 3rd Reagent Project group C that the integrated Reagent Tube 200 of Anti-HBc Serum five is formed blocks location in the slot 50 on the rotating disk 30 advancing corresponding 1st track position by the first actuator 60.
3rd Reagent Project group C is detained reaction minute 1 minute respectively in the 1st, 2 track positions.
3rd Reagent Project group C passes through pressue device 90 malleation mode respectively by HBsAg in the 3rd track position, Anti-HBsAg antibody, and HBeAg, anti-HBe, in the integrated Reagent Tube 200 of Anti-HBc Serum five, sample press filtration is to the micropore cup 201 inner bottom part reaction zone of integrated Reagent Tube 200.
3rd Reagent Project group C moves to the 4th, 5 track positions, each delay 1 minute.
3rd Reagent Project group C adds reactant liquor by liquid-adding device 100 in each integrated Reagent Tube 200 when the 6th track position.
Reaction zone bottom the initial reactant press filtration that reactant liquor washs in the 8th track position by the 3rd Reagent Project group C by each integrated Reagent Tube 200 to micropore cup 201.
Each integrated Reagent Tube 200 top filtration unit 202 takes out in the 9th, 10,11 any track positions and abandons by the 3rd Reagent Project group C.
Period: the 3rd Reagent Project group C moves to the 9th, 10,11,12 track positions and is respectively detained 1 minute.
3rd Reagent Project group C is after the 12nd road is detained end in 1 minute, and the 3rd Reagent Project group C entirety is released slot 50 by the second actuator 70 and entered washing link.
Above embodiment is used for illustrative purposes only, but not the present invention is limited, those skilled in the art, without departing from the spirit and scope of the present invention, various conversion or change can also be made, therefore, all equivalent technical schemes also should belong to category of the present invention, are limited by each claim.
Claims (8)
1. any project team of immune detection parallel reaction treating apparatus, comprise a rotating disk (30) driven by time delay stepping motor rotating shaft (20), rotating disk (30) center outwards arranges some fan-shaped stations (40) radially, is provided with a slot (50) of locating for the plant of Reagent Project group in each fan-shaped station (40); Being provided with at outside two ends, rotating disk (30) footpath can first actuator (60) of Reagent Project group side direction drive slot (50) and the second actuator (70) releasing slot (50);
Rotate clockwise on direction at rotating disk (30), the second actuator (70) is vacant district (300) to rotating disk (30) region of the first actuator (60); First actuator (60) is the reaction zone (400) of many Reagent Project group parallel processing to rotating disk (30) region of the second actuator (70);
To rotate clockwise at rotating disk (30) on the reaction zone (400) on direction also sequentially interval arrange provide a pressue device (90) of source of the gas to the measuring samples press filtration in Reagent Project group, Reagent Project group provided to a liquid-adding device (100) of reactant liquor, pressue device (90) that a pair reactant liquor press filtration provides source of the gas.
2. any project team of a kind of immune detection as claimed in claim 1 parallel reaction treating apparatus, it is characterized in that: described each Reagent Project group is made up of side by side at least more than one integrated Reagent Tube (200), each slot (50) two side is arranged with the arc-shaped slot (51) compressing integrated Reagent Tube outer wall.
3. any project team of a kind of immune detection as claimed in claim 2 parallel reaction treating apparatus, it is characterized in that: described integrated Reagent Tube (200) comprises one can immobilised micropore cup (201) and one and micropore cup (201) the mouth place dismounting sample filtration device (202) of joining in advance, arrange the sample filter bowl (204) that micropore cup (201) is inserted in a bottom in the middle of sample filtration device (202), sample filter bowl (204) surrounding is connected on sample filtration device (202) by some every beam (203); Sample filter bowl (204) bottom surface is provided with the minim pipette (205) be communicated with inside and outside sample filter bowl (204).
4. any project team of a kind of immune detection as claimed in claim 3 parallel reaction treating apparatus, is characterized in that: described sample filter bowl (204) inner bottom surface is fixed with from top to bottom by sample filter membrane (208) and the stacked binding film (207) formed of label film (209) by snap ring (206).
5. any project team of a kind of immune detection as claimed in claim 1 parallel reaction treating apparatus, it is characterized in that: described pressue device (90) comprises the peripheral vertical pole (91) of a fixing rotating disk (30), the cross bar (92) and on fixing vertical pole (91) top can be fixed a point the slide block (93) that slides along cross bar (92) on slot (50), slide block (93) top is provided with a gas-tpe fitting (931) be connected with air supply hose (933), arranges and communicate and the gas-adding pipe (932) of automatically retractable with gas-tpe fitting (931) under slide block (93).
6. any project team of a kind of immune detection as claimed in claim 5 parallel reaction treating apparatus, is characterized in that: described cross bar (92) is provided with the block (921) limiting slide block (93) sliding scale directly over slot (50).
7. any project team of a kind of immune detection as claimed in claim 1 parallel reaction treating apparatus, it is characterized in that: described liquid-adding device (100) comprises the peripheral vertical rod (101) of a fixing rotating disk (30), the slide bar (102) and one on one fixing vertical rod (101) top can be fixed a point the sliding sleeve (103) that slides along slide bar (102) on slot (50), sliding sleeve (103) top is provided with a liquid pipe connection (1031) be connected with feed liquid hose (1033), arrange under sliding sleeve (103) and communicate and the liquid injection pipe (1032) of automatically retractable with liquid pipe connection (1031).
8. any project team of a kind of immune detection as claimed in claim 7 parallel reaction treating apparatus, is characterized in that: described slide bar (102) is provided with the limited block (1021) limiting sliding sleeve (103) sliding scale directly over slot (50).
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| CN201510740007.1A CN105301227B (en) | 2015-11-04 | 2015-11-04 | A kind of any project team's parallel reaction processing meanss of immune detection |
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| CN201510740007.1A CN105301227B (en) | 2015-11-04 | 2015-11-04 | A kind of any project team's parallel reaction processing meanss of immune detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680479A (en) * | 2017-03-09 | 2017-05-17 | 厦门先明生物技术有限公司 | Parallel detection and arbitrary combination integrator for multi-index integrated reagents |
| CN117723743A (en) * | 2023-12-18 | 2024-03-19 | 浙江华芯云生物科技有限公司 | Full-automatic reaction waiting mechanism of immunochromatography detection card and using method |
Citations (6)
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| CN105301227B (en) | 2017-03-29 |
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Effective date of registration: 20191114 Address after: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289 building third unit 10 branch Patentee after: XIAMEN XIANMING BIOTECHNOLOGY CO., LTD. Address before: Siming District Yuqing road Xiamen City, Fujian province 361003 No. 33 building B room 1302 Patentee before: Xu Enliang |