CN105198095A - Treatment method of degrading xylene in wastewater by immobilization of microalgae of fungus medium - Google Patents
Treatment method of degrading xylene in wastewater by immobilization of microalgae of fungus medium Download PDFInfo
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- CN105198095A CN105198095A CN201510694267.XA CN201510694267A CN105198095A CN 105198095 A CN105198095 A CN 105198095A CN 201510694267 A CN201510694267 A CN 201510694267A CN 105198095 A CN105198095 A CN 105198095A
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Abstract
The invention discloses a treatment method of degrading xylene in wastewater by immobilization of microalgae of fungus medium. The treatment method comprises the following steps: carrying out degradation treatment on xylene in water by adopting microorganisms, wherein the microorganisms are spheres in which microalgae and fungi form microalgae symbiosis. Through adopting the microalgae and the fungi to form symbiotic spheres, the advantages of being simple and efficient, low in cost, good in contact interface property, capable of effectively degrading xylene in water, capable of avoid the use of a chemical reagent, and eco-friendly can be realized.
Description
Technical field
The present invention relates to a kind of waste water specific pollutants treatment process, particularly relate to the treatment process of p-Xylol in micro-algae immobilization degrading waste water of fungi medium.
Background technology
P-Xylol is important industrial chemicals, can be used as the thinner of the thinner of pigment, paint etc. etc., the solvent of the industry such as printing, rubber, leather.In the production process of the relevant industries such as organic synthesis, synthetic rubber, paint and dyestuff, all can produce a large amount of dimethylbenzene waste water and gas, these dimethylbenzene waste water and waste gas can enter water body with the waste water that it is produced and applying unit is discharged.In recent years, the direct discharge of trade effluent, causes the organic contamination that water body is serious, although dimethylbenzene in environment can biological degradation, biodegradation rate is low many more than rate of volatilization.Therefore, the discharge of dimethylbenzene waste water not only threatens fish and hydrobiological existence, and the health of the mankind in serious threat.
In recent years, large quantity research has been carried out to the potentiality playing algae purification sewage further both at home and abroad, had made great progress in the study mechanism of algae purification sewage.Compared with traditional method, the drawbacks such as the secondary pollution utilizing algae to dispose of sewage can to overcome traditional wastewater treatment process easily to cause, potential nutritive substance are run off, resource can not utilize completely, can effectively and at low cost remove causes p-Xylol to the pollution of water quality simultaneously, therefore has broad application prospects.
Summary of the invention
The object of the invention is to for prior art provide a kind of there is fungi-micro-algae symbiosis macrobead spheroid, simply efficient, with low cost, contact interface performance is good, the treatment process of p-Xylol in micro-algae immobilization degrading waste water of ecological friendly fungi medium.
The present invention solves the problems of the technologies described above adopted technical scheme: the treatment process of p-Xylol in micro-algae immobilization degrading waste water of fungi medium, comprise and adopt microorganism to carry out degradation treatment to the dimethylbenzene in water, wherein: microorganism is the spheroid that micro-algae and fungi form micro-algae symbiosis.Adopt micro-algae and fungi to form symbiosis spheroid, have simply efficient, with low cost, contact interface performance is good, can dimethylbenzene in effective degradation water, and the use of chemical reagent can be avoided, ecological friendly.
For optimizing technique scheme, the measure taked also comprises: proceed to bio-reactor high-density culture after being cultivated under autotrophy culture condition or Heterotrophic culture condition by micro-algae; Fungal spore is added in micro-algae liquid of bio-reactor high-density culture until form the spheroid of micro-algae and mycosymbiosis.Fungal spore enters micro-algae liquid through reaction contact after a while, can form the spheroid of symbiosis, thus have the effect of degraded dimethylbenzene beyond expectation.Micro-algae comprises Chlorella, simple post Trentepohlia, diatom, rhombus algae, splits kettle algae, Dunaliella, Nannochloropsis oceanica, Chlamydomonas, flat algae, empty ball Trentepohlia wherein one or more; The substratum that micro-algae adopts comprises BG-11 substratum or F/2 substratum or walne substratum wherein one or more.Experimentally, preferred appropriate culture medium can form the spherical Symbiont corresponding to experiment purpose.The spore of the fungal bacterial strain of fungus culture self energy balling-up; The fungal bacterial strain of balling-up comprises meegan enzyme, variable color auricularia auriculajudae, oyster cap fungus, morel, Penicllium chrysogenum, Twospore Mushroom, Mucor, Mortierella isabellina, marine alga aspergillus, Trichodermareesei, the flat lead fungi of yellow spore wherein one or more.Above-mentioned bacterial classification has certain Traits change, but all have growth fast, form Symbiont after effectively to degrade the effect of the dimethylbenzene removed in water body, and can Reusability.Autotrophy culture condition is: temperature controls in the scope of 20-45 DEG C, and the starting point concentration of illumination cultivation nitrogenous source glycine or yeast extract, between 1-15g/L, passes into the mixed gas of air or air and carbonic acid gas, and air flow is 50-300L/h.Gas concentration lwevel is 0.9-3%; Culturing process adopts 10-200umolm
-2s
-1sun exposure, pH controls between 5-9; Total incubation time is between 50-400h.Adopt specific autotrophy culture condition to coordinate corresponding bacterial strain, the symbiosis spheroid of formation, surface bonding is even, forms spheroid and stablizes.Heterotrophism condition is: organic carbon source glucose is 20g/L; Air flow 150-250L/h, in culturing process, pH value is 6 to 8, and total incubation time is 100-150h.The fractional yield of heterotrophism CMC model is stablized, and forms spheroid effect also no significant difference.
Be the spheroid that micro-algae and fungi form micro-algae symbiosis owing to present invention employs microorganism.Adopt micro-algae and fungi to form symbiosis spheroid, have simply efficient, with low cost, contact interface performance is good, can dimethylbenzene in effective degradation water, and the use of chemical reagent can be avoided, ecological friendly.Thus the present invention have fungi-micro-algae symbiosis macrobead spheroid, simply efficient, with low cost, contact interface performance is good, ecological friendly advantage.
Embodiment
Below in conjunction with attached embodiment, the present invention is described in further detail.
Embodiment 1: the treatment process of p-Xylol in micro-algae immobilization degrading waste water of fungi medium, comprises and adopts microorganism to carry out degradation treatment to the dimethylbenzene in water, wherein: microorganism is the spheroid that micro-algae and fungi form micro-algae symbiosis.Adopt micro-algae and fungi to form symbiosis spheroid, have simply efficient, with low cost, contact interface performance is good, can dimethylbenzene in effective degradation water, and the use of chemical reagent can be avoided, ecological friendly.Bio-reactor high-density culture is proceeded to after being cultivated under autotrophy culture condition or Heterotrophic culture condition by micro-algae; Fungal spore is added in micro-algae liquid of bio-reactor high-density culture until form the spheroid of micro-algae and mycosymbiosis.Fungal spore enters micro-algae liquid through reaction contact after a while, can form the spheroid of symbiosis, thus have the effect of degraded dimethylbenzene beyond expectation.Micro-algae comprises Chlorella, simple post Trentepohlia, diatom, rhombus algae, splits kettle algae, Dunaliella, Nannochloropsis oceanica, Chlamydomonas, flat algae, empty ball Trentepohlia wherein one or more; The substratum that micro-algae adopts comprises BG-11 substratum or F/2 substratum or walne substratum wherein one or more.Experimentally, preferred appropriate culture medium can form the spherical Symbiont corresponding to experiment purpose.The spore of the fungal bacterial strain of fungus culture self energy balling-up; The fungal bacterial strain of balling-up comprises meegan enzyme, variable color auricularia auriculajudae, oyster cap fungus, morel, Penicllium chrysogenum, Twospore Mushroom, Mucor, Mortierella isabellina, marine alga aspergillus, Trichodermareesei, the flat lead fungi of yellow spore wherein one or more.Above-mentioned bacterial classification has certain Traits change, but all have growth fast, form Symbiont after effectively to degrade the effect of the dimethylbenzene removed in water body, and can Reusability.Autotrophy culture condition is: temperature controls in the scope of 20-45 DEG C, and the starting point concentration of illumination cultivation nitrogenous source glycine or yeast extract, between 1-15g/L, passes into the mixed gas of air or air and carbonic acid gas, and air flow is 50-300L/h.Gas concentration lwevel is 0.9-3%; Culturing process adopts 10-200umolm
-2s
-1sun exposure, pH controls between 5-9; Total incubation time is between 50-400h.Adopt specific autotrophy culture condition to coordinate corresponding bacterial strain, the symbiosis spheroid of formation, surface bonding is even, forms spheroid and stablizes.Heterotrophism condition is: organic carbon source glucose is 20g/L; Air flow 150-250L/h, in culturing process, pH value is 6 to 8, and total incubation time is 100-150h.The fractional yield of heterotrophism CMC model is stablized, and forms spheroid effect also no significant difference.
Further, the present invention is further illustrated by example.
(1) switching of micro-algae: take out the strain of frozen micro-algae algae from-70 DEG C of refrigerators and use transfering loop scraping a little to the dull and stereotyped illumination cultivation of solid slope.
Autotrophy culture condition is as follows: temperature controls at 20 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 1g/L, passes into air or air and CO
2mixed gas, air flow 50L/h; CO
2concentration 0.9%.10umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls between 5; Total incubation time 50 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 0.01; Pass into air, air flow 100L/h; 5umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 72 hours.
(2) high-density culture of micro-algae: plate culture is seeded to bioreactor culture, cultivates at Heterotrophic culture base middle-high density; Biological reaction apparatus comprises shaking flask, ventilation bottle, bioreactor, fermentor tank and open culture pond.Cultivate at above-mentioned suitable condition, until cell log vegetative period, cell density reaches (more than 106-1010).
Autotrophy culture condition is as follows: temperature to control at 20 DEG C of illumination cultivation nitrogenous sources as the starting point concentration such as glycine, yeast extract 1g/L, passes into air or air and CO
2mixed gas, air flow 50L/h; CO
2concentration 0.9%.10umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 50 hours.
Heterotrophic culture condition is as follows: organic carbon source, as glucose concn 0.01g/L, passes into air, air flow 100L/h; 5umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 72h
3) cultivation of the spore of the fungi of energy balling-up: take out frozen fungal bacterial strain from-70 DEG C of refrigerators and cultivate 4-7 days to solid plate a little by transfering loop scraping, then repeatedly rinses with sterilized water and obtains fresh fungal spore.
(4) add in Fresh spores suspension to micro-algae culture medium and cultivate cell log vegetative period at microalgae cell, cell density reaches (10
6-10
10above), the fungal spore of some amount is added in the substratum of Xiang Weizao.
(5) fungi mediation microalgae cell immobilization: optimum culture condition, causes until all micro-algaes and fungi form the spheroid of micro-algae symbiosis.Form micro-algae of micro-algae symbiosis spheroid, carry out microalgae harvesting with simple filter method.
(6) immobilized microalgae cell is used for the p-Xylol in degrading waste water.
Embodiment 2:
(1) switching of micro-algae: take out the strain of frozen micro-algae algae from-70 DEG C of refrigerators and use transfering loop scraping a little to the dull and stereotyped illumination cultivation of solid slope.
Autotrophy culture condition is as follows: temperature controls at 45 DEG C, and illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 15g/L, passes into air or air and CO
2mixed gas, air flow 50L/h; CO
2concentration 3%.10umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5, total incubation time 50 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 0.01g/L; Pass into air, air flow 100L/h; 5umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time visual cell grows 72 hours.
(2) high-density culture of micro-algae: plate culture is seeded to bioreactor culture, cultivates at Heterotrophic culture base middle-high density; Biological reaction apparatus comprises shaking flask, ventilation bottle, bioreactor, fermentor tank and open culture pond.Cultivate at above-mentioned suitable condition, until cell log vegetative period, cell density reaches (more than 106-1010).
Autotrophy culture condition is as follows: temperature controls at 45 DEG C, and illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 1g/L, passes into air or air and CO
2mixed gas, air flow 50L/h; CO
2concentration 3%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 9 total incubation time 120 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 200g/L; Pass into air, air flow 400L/h; 40umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 200 hours.
(3) cultivation of the spore of the fungi of energy balling-up: take out frozen fungal bacterial strain from-70 DEG C of refrigerators and cultivate 4-7 days to solid plate a little by transfering loop scraping, then repeatedly rinses with sterilized water and obtains fresh fungal spore.
(4) add in Fresh spores suspension to micro-algae culture medium and cultivate cell log vegetative period at microalgae cell, cell density reaches (more than 106-1010), adds the fungal spore of some amount in the substratum of Xiang Weizao.
(5) fungi mediation microalgae cell immobilization: optimum culture condition, causes until all micro-algaes and fungi form the spheroid of micro-algae symbiosis.Form micro-algae of micro-algae symbiosis spheroid, carry out microalgae harvesting with simple filter method.
(6) immobilized microalgae cell is used for the p-Xylol in degrading waste water.
Embodiment 3:
(1) switching of micro-algae: take out the strain of frozen micro-algae algae from-70 DEG C of refrigerators and use transfering loop scraping a little to the dull and stereotyped illumination cultivation of solid slope.
Autotrophy culture condition is as follows: temperature controls at 45 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 4g/L, passes into air or air and CO
2mixed gas, air flow 300L/h; CO
2concentration 3%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 9; Total incubation time 200 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 20g/L; Pass into air, air flow 300L/h; 40umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 72 hours.
(2) high-density culture of micro-algae: plate culture is seeded to bioreactor culture, cultivates at Heterotrophic culture base middle-high density; Biological reaction apparatus comprises shaking flask, ventilation bottle, bioreactor, fermentor tank and open culture pond.Cultivate at above-mentioned suitable condition, until cell log vegetative period, cell density reaches (more than 106-1010).
Autotrophy culture condition is as follows: temperature controls at 20 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 1g/L, passes into air or air and CO
2mixed gas, air flow 50L/h; CO
2concentration 0.9%.10umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 50 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 0.01g/L; Pass into air, air flow 100L/h, preferred 150-250L/h; 5umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time looks 72 hours.
(3) cultivation of the spore of the fungi of energy balling-up: take out frozen fungal bacterial strain from-70 DEG C of refrigerators and cultivate 4-7 days to solid plate a little by transfering loop scraping, then repeatedly rinses with sterilized water and obtains fresh fungal spore.
(4) add in Fresh spores suspension to micro-algae culture medium and cultivate cell log vegetative period at microalgae cell, cell density reaches (10
6-10
10above), the fungal spore of some amount is added in the substratum of Xiang Weizao.
(5) fungi mediation microalgae cell immobilization: optimum culture condition, causes until all micro-algaes and fungi form the spheroid of micro-algae symbiosis.Form micro-algae of micro-algae symbiosis spheroid, carry out microalgae harvesting with simple filter method.
(6) immobilized microalgae cell is used for the p-Xylol in degrading waste water.
Embodiment 4:
(1) switching of micro-algae: take out the strain of frozen micro-algae algae from-70 DEG C of refrigerators and use transfering loop scraping a little to the dull and stereotyped illumination cultivation of solid slope.
Autotrophy culture condition is as follows: temperature controls at 20 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 4g/L, passes into air or air and CO
2mixed gas, air flow 300L/h; CO
2concentration 3%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 200 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 0.01g/L; Pass into air, air flow 400L/h; 5umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 72 hours.
(2) high-density culture of micro-algae: plate culture is seeded to bioreactor culture, cultivates at Heterotrophic culture base middle-high density; Biological reaction apparatus comprises shaking flask, ventilation bottle, bioreactor, fermentor tank and open culture pond.Cultivate at above-mentioned suitable condition, until cell log vegetative period, cell density reaches (more than 106-1010).
Autotrophy culture condition is as follows: temperature controls at 20 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 15g/L, passes into air or air and CO
2mixed gas, air flow 300L/h; CO
2concentration 0.9%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 8; Total incubation time 120 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 200g/L; Pass into air, air flow 400L/h; 40umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 7.0; Total incubation time 72 hours.
(3) cultivation of the spore of the fungi of energy balling-up: take out frozen fungal bacterial strain from-70 DEG C of refrigerators and cultivate 4-7 days to solid plate a little by transfering loop scraping, then repeatedly rinses with sterilized water and obtains fresh fungal spore.
(4) add in Fresh spores suspension to micro-algae culture medium and cultivate cell log vegetative period at microalgae cell, cell density reaches (10
6-10
10above), the fungal spore of some amount is added in the substratum of Xiang Weizao.
(5) fungi mediation microalgae cell immobilization: optimum culture condition, causes until all micro-algaes and fungi form the spheroid of micro-algae symbiosis.Form micro-algae of micro-algae symbiosis spheroid, carry out microalgae harvesting with simple filter method.
(6) immobilized microalgae cell is used for the p-Xylol in degrading waste water.
Embodiment 5:
(1) switching of micro-algae: take out the strain of frozen micro-algae algae from-70 DEG C of refrigerators and use transfering loop scraping a little to the dull and stereotyped illumination cultivation of solid slope.
Autotrophy culture condition is as follows: temperature controls at 45 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 15g/L, passes into air or air and CO
2mixed gas, air flow 150L/h; CO
2concentration 2.5%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 7; Total incubation time 50 hours.
Heterotrophic culture condition is as follows: organic carbon source is as glucose concn 200g/L; Pass into air, air flow 100L/h; 40umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 100 hours.
(2) high-density culture of micro-algae: plate culture is seeded to bioreactor culture, cultivates at Heterotrophic culture base middle-high density; Biological reaction apparatus comprises shaking flask, ventilation bottle, bioreactor, fermentor tank and open culture pond.Cultivate at above-mentioned suitable condition, until cell log vegetative period, cell density reaches (10
6-10
10above).
Autotrophy culture condition is as follows: temperature controls at 20 DEG C; Illumination cultivation nitrogenous source, as the starting point concentration such as glycine, yeast extract 15g/L, passes into air or air and CO
2mixed gas, air flow 300L/h; CO
2concentration 0.9%.200umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 9; Total incubation time 150 hours.
Heterotrophic culture condition is as follows: organic carbon source as glucose concn 20g/L; Pass into air, air flow 200L/h; 40umol.m is adopted in culturing process
-2.s
-1sun exposure, pH value controls 5; Total incubation time 120 hours.
(3) cultivation of the spore of the fungi of energy balling-up: take out frozen fungal bacterial strain from-70 DEG C of refrigerators and cultivate 4-7 days to solid plate a little by transfering loop scraping, then repeatedly rinses with sterilized water and obtains fresh fungal spore.
(4) add in Fresh spores suspension to micro-algae culture medium and cultivate cell log vegetative period at microalgae cell, cell density reaches (10
6-10
10above), the fungal spore of some amount is added in the substratum of Xiang Weizao.
(5) fungi mediation microalgae cell immobilization: optimum culture condition, causes until all micro-algaes and fungi form the spheroid of micro-algae symbiosis.Form micro-algae of micro-algae symbiosis spheroid, carry out microalgae harvesting with simple filter method.
(6) immobilized microalgae cell is used for the p-Xylol in degrading waste water.
The test of degraded catalytic capability:
Get certain density p-Xylol aqueous solution 500mL, cultured micro-algae 100mL is joined in this aqueous solution.Carry out the real time measure to para-xylene concentration in the aqueous solution, record data are as shown in the table.
Although describe the present invention in conjunction with preferred embodiment; so itself and be not used to limit the present invention; any those skilled in the art; without departing from the spirit and scope of the present invention; can implement various change, the displacement of coordinator and amendment here to the theme listed, therefore protection scope of the present invention be as the criterion when the scope limited depending on proposed claim.
Claims (6)
1. the treatment process of p-Xylol in micro-algae immobilization degrading waste water of fungi medium, comprises and adopts microorganism to carry out degradation treatment to the dimethylbenzene in water, it is characterized in that: described microorganism is the spheroid that micro-algae and fungi form micro-algae symbiosis.
2. in micro-algae immobilization degrading waste water of fungi medium according to claim 1, the treatment process of p-Xylol, is characterized in that: proceed to bio-reactor high-density culture after being cultivated under autotrophy culture condition or Heterotrophic culture condition by described micro-algae; Fungal spore is added in micro-algae liquid of described bio-reactor high-density culture until form the spheroid of micro-algae and mycosymbiosis.
3. in micro-algae immobilization degrading waste water of fungi medium according to claim 1, the treatment process of p-Xylol, is characterized in that: described micro-algae comprises Chlorella, simple post Trentepohlia, diatom, rhombus algae, splits kettle algae, Dunaliella, Nannochloropsis oceanica, Chlamydomonas, flat algae, empty ball Trentepohlia wherein one or more; The substratum that described micro-algae adopts comprises BG-11 substratum or F/2 substratum or walne substratum wherein one or more.
4. in micro-algae immobilization degrading waste water of fungi medium according to claim 1, the treatment process of p-Xylol, is characterized in that: the spore of the fungal bacterial strain of described fungus culture self energy balling-up; The fungal bacterial strain of described balling-up comprises meegan enzyme, variable color auricularia auriculajudae, oyster cap fungus, morel, Penicllium chrysogenum, Twospore Mushroom, Mucor, Mortierella isabellina, marine alga aspergillus, Trichodermareesei, the flat lead fungi of yellow spore wherein one or more.
5. the treatment process of p-Xylol in micro-algae immobilization degrading waste water of fungi medium according to claim 1, it is characterized in that: described autotrophy culture condition is: temperature controls in the scope of 20-45 DEG C, the starting point concentration of illumination cultivation nitrogenous source glycine or yeast extract is between 1-15g/L, pass into the mixed gas of air or air and carbonic acid gas, air flow is 50-300L/h; Gas concentration lwevel is 0.9-3%; Culturing process adopts 10-200umol.m
-2.s
-1sun exposure, pH controls between 5-9; Total incubation time is between 50-400 hour.
6. in micro-algae immobilization degrading waste water of fungi medium according to claim 1, the treatment process of p-Xylol, is characterized in that: described heterotrophism condition is: organic carbon source glucose is 20g/L; Air flow 150-250L/h, in culturing process, pH value is 6 to 8, and total incubation time is 100-150 hour.
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| EP4153547A4 (en) * | 2020-05-22 | 2024-06-26 | Algaenergy N.A. Inc. | Microalgae enhanced biological crop nutrition granules |
| CN116333893A (en) * | 2023-03-23 | 2023-06-27 | 大理大学 | Fungus for degrading o-xylene and application thereof |
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