CN105145560A - New application of 1-octene-3-ol as bactericide or bacteriostatic agent - Google Patents
New application of 1-octene-3-ol as bactericide or bacteriostatic agent Download PDFInfo
- Publication number
- CN105145560A CN105145560A CN201510634635.1A CN201510634635A CN105145560A CN 105145560 A CN105145560 A CN 105145560A CN 201510634635 A CN201510634635 A CN 201510634635A CN 105145560 A CN105145560 A CN 105145560A
- Authority
- CN
- China
- Prior art keywords
- ocol
- concentration
- bactericide
- fusarium
- disease fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide and provides new application of 1-octene-3-ol as bactericide or a bacteriostatic agent. The 1-octene-3-ol has the advantages that the 1-octene-3-ol has a remarkable preventing function on fungal diseases or bacterial diseases of plants, pesticide resistance is not prone to being formed, and the 1-octene-3-ol is safe to people and livestock and the environment. An efficient, non-toxic, safe and residue-free bacterium killing and inhibiting method is provided for common pathogenic fungi and bacteria of agriculture and forestry. The 1-octene-3-ol is efficient, wide in fungicide spectrum, easy to volatize in the environment, free of residues, harmless to people and livestock and capable of preventing pathogenic fungi, saccharomycetes and bacteria of fruit trees and soil. The inhibiting rate on spore germination and mycelial growth of fusarfum tricinctum and fusarium oxysporum is higher than 95%. The inhibiting effect on bacillus subtitles, escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and the like is remarkable.
Description
Technical field
The invention belongs to technical field of pesticide, there is provided the novelty teabag that 1-OCOL is applied as bactericide or bacteriostatic agent, 1-OCOL has preventive effect to the fungal disease of plant or bacterial disease and significantly, not easily develops immunity to drugs and advantage safe for human and animal and environment.
Background technology
Apple, banana, mango etc. are the Important Economic crops of China, to drive local economy, promote that increasing peasant income play an important role.But the damage by disease and insect such as dry rot, charcoal myiasis spread unchecked the productive life having had a strong impact on peasant, constrain the lifting of crop yield and quality.In many countries such as China, the U.S., green, the safety of crops receive increasing concern.Bactericide, as the efficient servicing unit of one, promotes to play at the cultivation management of crops and output and to a certain degree acts on.But existing bactericide, mostly tanginess, inconvenience is applied to human settlement, and easily remains, and can work the mischief to people and animals, therefore have impact on the foreign exchange earning of Important Economic crop.
1-OCOL, English name 1-Octen-3-ol; 3-Octenol.Have another name called mushroom alcohol (Mushroomalcohol), vinyl amyl carbinol (Vinylpentylcarbinol) or 1-vinyl hexanol (1-Vinylhexanol).Molecular formula is C
8h
16o, structural formula is CH
2=CHCH (OH) CH
2cH
2cH
2cH
2cH
3.1-OCOL is aliphatic unsaturated alcohol, and outward appearance is colourless to pale yellow oily liquid body, has mushroom, lavender, rose and hay fragrance.Occurring in nature, is mainly present in pennyroyal, thyme and fresh mushroom, belongs to natural equivalent spices.In the numerous food product such as the fruits such as banana and berry, beer, pork, river prawn, soybean, also trace contains.Water insoluble, solvable with organic solvents such as ethanol.Industrially produce 1-OCOL by synthesis, instead of be separated from natural goods and produce.
Main application is modulation fragrance and pharmaceutical raw material: (1), for daily use chemicals and flavoring essence, GB2760-1996 is defined as the flavorant allowing to use.Can be used for the preparation type such as mushroom and Earthy Taste essence, artificial essential oil, the essential oil or make ester perfume of recombinating.(2) as chemosynthesis raw material, pharmaceutical field is applied to; As mosquito attractant, be applied to and kill mosquito goods.There is not yet the report that it has sterilization or bacteriostasis efficacy.
Summary of the invention
First technical problem solved by the invention is to provide the novelty teabag of 1-OCOL.
Concrete, the invention provides the novelty teabag that 1-OCOL is applied as bactericide or bacteriostatic agent.
Concrete, 1-OCOL can suppress or kill disease fungus or pathogenetic bacteria.
Described pathogenetic bacteria is Staphylococcus epidermidis, staphylococcus albus, bacillus subtilis, side bacillus, pseudomonas aeruginosa, aurococcus, Escherichia coli, Bacillus cereus, bacillus thuringiensis, Agrobacterium tumefaciems.
Described disease fungus is fusarium tricinctum, Fusarium oxysporum, Fusarium graminearum, fusarium moniliforme, Fusarinm solani.
Concrete, for pathogenetic bacteria, in order to realize clear and definite, good antibacterial or bactericidal effect, the concentration of 1-OCOL is at least 1.25 μ l/ml.Under this concentration, 1-OCOL all has significant inhibition to pathogenetic bacteria Staphylococcus epidermidis, staphylococcus albus, bacillus subtilis, side bacillus, pseudomonas aeruginosa.The concentration of preferred 1-OCOL is at least 2.5 μ l/ml; Most preferably the concentration of 1-OCOL is at least 5 μ l/ml.
Known by the ne ar of scanning electron microscopic observation process, 1-OCOL, by the cell membrane of destroy microorganisms, causes microbial death.And detect discovery, through the microorganism of 1-OCOL process, its reducing sugar, protein, electrolyte can be discharged in culture fluid in a large number, also can illustrate because cell membrane is destroyed the death that result in bacterium, and then realize antibacterial or biocidal efficacies.
Concrete, for disease fungus, in order to realize clear and definite, good antibacterial or bactericidal effect, the concentration of 1-OCOL is at least 1.25 μ l/ml.Adopt 1-OCOL can realize suppressing spore germination or the mycelial growth of disease fungus.Preferred further, when the concentration of 1-OCOL is at least 2.5 μ l/ml, the spore germination of fusarium tricinctum, Fusarium oxysporum can be suppressed completely; When the concentration of 1-OCOL is at least 5 μ l/ml, fusarium tricinctum can be suppressed completely, the mycelial growth of Fusarium oxysporum.
Second technical problem solved by the invention is to provide a kind of bactericide or bacteriostatic agent, and the 1-OCOL that it includes effective amount is active component.
Concrete, in order to realize the effect killing or suppress pathogenetic bacteria, in bactericide or bacteriostatic agent, the concentration of 1-OCOL is for being at least 1.25 μ l/ml.The concentration of preferred 1-OCOL is at least 2.5 μ l/ml; Most preferably the concentration of 1-OCOL is at least 5 μ l/ml.
Concrete, in order to realize the effect killing or suppress disease fungus, in bactericide or bacteriostatic agent, the concentration of 1-OCOL is for being at least 1.25 μ l/ml.The concentration of preferred 1-OCOL is at least 2.5 μ l/ml; Most preferably the concentration of 1-OCOL is at least 5 μ l/ml.
Wherein: when killing or suppress disease fungus, suppress the spore germination of fusarium tricinctum, Fusarium oxysporum completely, the concentration of 1-OCOL is at least 2.5 μ l/ml; Suppress fusarium tricinctum completely, the mycelial growth of Fusarium oxysporum, the concentration of 1-OCOL is at least 5 μ l/ml.
In above-mentioned bactericide or bacteriostatic agent, solvent is water.
Further, suitable organic co-solvents can be added and promote that 1-OCOL dissolves.
Described organic co-solvents is absolute ethyl alcohol, glycerine, glycerine, polyethylene glycol.
The addition of organic co-solvents is 0.5-1% (percentage by weight).
3rd technical problem solved by the invention is to provide the preparation method of a kind of bactericide or bacteriostatic agent, comprises the steps:
A, to take 1-OCOL be active component;
B, be at least 1.25 μ l/ml by solvent dilution to the concentration of 1-OCOL.
Wherein, described solvent is water.
Further, suitable organic co-solvents can be added and promote that 1-OCOL dissolves.
Described organic co-solvents is absolute ethyl alcohol, glycerine, glycerine, polyethylene glycol.
The addition of organic co-solvents is 0.5-1% (percentage by weight).
4th technical problem solved by the invention is to provide the using method of a kind of bactericide or bacteriostatic agent, it is characterized in that: bactericide or bacteriostatic agent adopt pouring, the mode of sprinkling irrigation uses.
A kind of bactericidal method that the present invention is the common disease fungus of agriculture and forestry, bacterium provides efficient, nontoxic, safety, noresidue.The 1-OCOL that the present invention adopts is efficient, sterilization spectrum width, is easy to volatilization in the environment, noresidue, nontoxic to people and animals, can to fruit tree, and pathogenic soil fungi, yeast, bacterium prevent and treat.95% is greater than to three line reaping hooks, Fusarium oxysporum spore germination and mycelial growth inhibition rate; Remarkable inhibition is all had to bacillus subtilis, Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa etc.
Accompanying drawing explanation
Figure 11-octene-3-alcohol is to the inhibitory action photo of bacterium.
Bacterial cell morphological differences before and after the process of Figure 25 μ l/ml1-octene-3-alcohol.
Wherein, the left figure upper right of Fig. 1 is depicted as the inhibition zone that the 1-OCOL containing 10 μ l2.5 μ l/ml concentration produces, and bottom right is depicted as the contrast filter paper containing sterile water, and on culture dish, indicator bacteria is Staphylococcus epidermidis; Right figure upper right is depicted as the inhibition zone that the 1-OCOL containing 10 μ l2.5 μ l/ml concentration produces, and bottom right is depicted as the contrast filter paper containing sterile water, and on culture dish, indicator bacteria is bacillus subtilis.
Wherein, the left figure of Fig. 2 is depicted as 1-OCOL bacillus subtilis form before treatment, and right figure is depicted as the bacillus subtilis form after process.Completely destroyed by ne ar after 5 μ l/ml1-octene-3-alcohol process, cell wall cracking, clasmatosis, content discharges in a large number, cell death.
Embodiment
Below by way of bacteriostatic test, effective effect of the present invention is described.
One. Minimal inhibitory concentration
1, the concentration gradient of 1-OCOL is arranged: 0; 0.625 μ l/ml; 1.25 μ l/ml; 2.5 μ l/ml; 5 μ l/ml; A 10 μ l/ml6 concentration gradient, each test arranges 3 repetitions
2, indicator bacteria is selected: staphylococcus albus; Pseudomonas aeruginosa; Side bacillus; Staphylococcus epidermidis; Bacillus subtilis;
3, filter paper osmosis is adopted to survey minimum inhibitory concentration, concrete grammar is: get a small pieces circular filter paper (diameter 13mm) and be positioned over the dull and stereotyped central authorities of the PDA scribbling indicator bacteria bacterium liquid, then the treatment fluid drawing each concentration of 10 μ l adds on circular filter paper, cultivates 12h for 37 DEG C and surveys inhibition zone size.
The results are shown in Table 1 and Fig. 1:
Table 1 antibacterial circle diameter
| Indicator bacteria | 0μl/ml | 0.625μl/ml | 1.25μl/ml | 2.5μl/ml | 5μl/ml | 10μl/ml |
| Staphylococcus epidermidis | 13mm | 13.2 | 13.4 | 14.1 | 15.6 | 16.5 |
| Staphylococcus albus | 13mm | 13.4 | 13.5 | 14.3 | 15.9 | 16.9 |
| Bacillus subtilis | 13mm | 13.1 | 13.4 | 14.5 | 16.1 | 17.0 |
| Side bacillus | 13mm | 13.0 | 13.2 | 13.9 | 14.9 | 16.0 |
| Pseudomonas aeruginosa | 13mm | 13.5 | 13.7 | 14.6 | 15.8 | 16.5 |
Experimental result shows, 1-OCOL all has significant inhibition to pathogenetic bacteria Staphylococcus epidermidis, staphylococcus albus, bacillus subtilis, side bacillus, pseudomonas aeruginosa, and minimum inhibitory concentration is 2.5 μ l/ml.
Two. to the inhibition test of disease fungus
1, disease fungus fusarium tricinctum, Fusarium oxysporum from crop rot position be separated, then by morphology, molecular biology method qualification.
2, the 1-OCOL of each concentration gradient is added in PDA medium, be mixed with containing 0; 0.625 μ l/ml; 1.25 μ l/ml; 2.5 μ l/ml; 5 μ l/ml; The PDA plating medium of a 10 μ l/ml6 concentration gradient 1-OCOL, then three line reaping hooks and Fusarium oxysporum are got a fritter pure culture biscuits involvng inoculation to the PDA medium central authorities containing each concentration treatment fluid by hole knockout, every day surveys mycelial growth rate, calculates its inhibiting rate.
The results are shown in Table 2:
Table 21-octene-3-alcohol is to three line reaping hooks and Fusarium oxysporum percentage mycelial inhibition
| 0μl/ml | 0.625μl/ml | 1.25μl/ml | 2.5μl/ml | 5μl/ml | 10μl/ml | |
| Fusarium tricinctum | 0 | 29.6％ | 35.8％ | 44.9％ | 100％ | 100％ |
| Fusarium oxysporum | 0 | 15.5％ | 29.2％ | 82.4％ | 100％ | 100％ |
From result, when the concentration of 1-OCOL is 5 μ l/ml, fusarium tricinctum can be suppressed completely, the mycelial growth of Fusarium oxysporum.
Three. to disease fungus spore germination Inhibition test
1, certain density three line reaping hooks, Fusarium oxysporum spore suspension is first prepared.
2, with 0; 0.625 μ l/ml; 1.25 μ l/ml; 2.5 μ l/ml; 5 μ l/ml; After the 1-OCOL process of a 10 μ l/ml6 concentration gradient, examine under a microscope spore germination rate.
The results are shown in Table 3:
Table 31-octene-3-alcohol is on the impact of three line reaping hooks and Fusarium oxysporum spore germination rate
| 0μl/ml | 0.625μl/ml | 1.25μl/ml | 2.5μl/ml | 5μl/ml | 10μl/ml | |
| Fusarium tricinctum | 95％ | 45％ | 8％ | 0％ | 0％ | 0％ |
| Fusarium oxysporum | 98％ | 39％ | 11％ | 0％ | 0％ | 0％ |
From result, when the concentration of 1-OCOL is 2.5 μ l/ml, the spore germination of fusarium tricinctum, Fusarium oxysporum can be suppressed completely.
Four. antimicrobial mechanism
Known by the ne ar of scanning electron microscopic observation process, 1-OCOL, by the cell membrane of destroy microorganisms, causes microbial death.And can be found by detection, the microorganism of 1-OCOL process, its reducing sugar, protein, electrolyte can be discharged in culture fluid in a large number, the membranolysis of microorganism also can be described, cause microbial death.The results are shown in Figure 2.
To sum up, the present invention is the common disease fungus of agriculture and forestry, bacterium provides a kind of bactericidal method of efficient, nontoxic, safety, noresidue.The 1-OCOL that the present invention adopts is efficient, sterilization spectrum width, is easy to volatilization in the environment, noresidue, nontoxic to people and animals, can to fruit tree, and pathogenic soil fungi, yeast, bacterium prevent and treat.95% is greater than to three line reaping hooks, Fusarium oxysporum spore germination and mycelial growth inhibition rate; Remarkable inhibition is all had to bacillus subtilis, Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa etc.
Claims (10)
1.1-octene-3-alcohol is as bactericide or bacteriostatic agent application.
2. application according to claim 1, is characterized in that: 1-OCOL suppresses or kills disease fungus or pathogenetic bacteria.
3. application according to claim 1, is characterized in that: 1-OCOL suppresses or kills pathogenetic bacteria is the cell membrane destroying pathogenetic bacteria.
4. application according to claim 1, is characterized in that: 1-OCOL suppresses or kills disease fungus is the spore germination or the mycelial growth that suppress disease fungus.
5. the application according to any one of claim 2-4, is characterized in that: meet following any one:
Described pathogenetic bacteria is Staphylococcus epidermidis, staphylococcus albus, bacillus subtilis, side bacillus, pseudomonas aeruginosa, aurococcus, Escherichia coli, Bacillus cereus, bacillus thuringiensis, Agrobacterium tumefaciems;
Described disease fungus is fusarium tricinctum, Fusarium oxysporum, Fusarium graminearum, fusarium moniliforme, Fusarinm solani.
6. the application according to any one of claim 2-4, is characterized in that: meet following any one:
The concentration suppressed or kill pathogenetic bacteria 1-OCOL is at least 1.25 μ l/ml;
Preferred suppression or the concentration killing pathogenetic bacteria 1-OCOL are at least 2.5 μ l/ml;
The concentration most preferably suppressed or kill pathogenetic bacteria 1-OCOL is at least 5 μ l/ml;
The concentration suppressed or kill disease fungus 1-OCOL is at least 1.25 μ l/ml;
Preferred suppression or the concentration killing disease fungus 1-OCOL are at least 2.5 μ l/ml;
The concentration most preferably suppressed or kill disease fungus 1-OCOL is at least 5 μ l/ml;
Suppress fusarium tricinctum completely, the concentration of spore germination 1-OCOL of Fusarium oxysporum is at least 2.5 μ l/ml;
Suppress fusarium tricinctum completely, the concentration of the mycelial growth 1-OCOL of Fusarium oxysporum is at least 5 μ l/ml.
7. bactericide or bacteriostatic agent, is characterized in that: the 1-OCOL that it includes effective amount is active component, and solvent is water;
Preferably, the organic co-solvents of 0.5-1% is added in solvent;
Preferred further, described organic co-solvents is absolute ethyl alcohol, glycerine, glycerine, polyethylene glycol.
8. bactericide according to claim 7 or bacteriostatic agent, is characterized in that: meet following any one:
The concentration suppressed or kill pathogenetic bacteria 1-OCOL is at least 1.25 μ l/ml;
Preferred suppression or the concentration killing pathogenetic bacteria 1-OCOL are at least 2.5 μ l/ml;
The concentration most preferably suppressed or kill pathogenetic bacteria 1-OCOL is at least 5 μ l/ml;
The concentration suppressed or kill disease fungus 1-OCOL is at least 1.25 μ l/ml;
Preferred suppression or the concentration killing disease fungus 1-OCOL are at least 2.5 μ l/ml;
The concentration most preferably suppressed or kill disease fungus 1-OCOL is at least 5 μ l/ml;
Suppress fusarium tricinctum completely, the concentration of spore germination 1-OCOL of Fusarium oxysporum is at least 2.5 μ l/ml;
Suppress fusarium tricinctum completely, the concentration of the mycelial growth 1-OCOL of Fusarium oxysporum is at least 5 μ l/ml.
9. the preparation method of bactericide or bacteriostatic agent, comprises the steps:
A, to take 1-OCOL be active component;
B, be at least 1.25 μ l/ml by solvent dilution to the concentration of 1-OCOL;
Preferably, described solvent is water;
Preferred further, add the organic co-solvents of 0.5-1% in solvent;
Preferably, described organic co-solvents is absolute ethyl alcohol, glycerine, glycerine, polyethylene glycol.
10. the using method of bactericide or bacteriostatic agent, is characterized in that: bactericide or bacteriostatic agent adopt pouring, the mode of sprinkling irrigation uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510634635.1A CN105145560A (en) | 2015-09-29 | 2015-09-29 | New application of 1-octene-3-ol as bactericide or bacteriostatic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510634635.1A CN105145560A (en) | 2015-09-29 | 2015-09-29 | New application of 1-octene-3-ol as bactericide or bacteriostatic agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105145560A true CN105145560A (en) | 2015-12-16 |
Family
ID=54786964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510634635.1A Pending CN105145560A (en) | 2015-09-29 | 2015-09-29 | New application of 1-octene-3-ol as bactericide or bacteriostatic agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105145560A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109221110A (en) * | 2018-10-01 | 2019-01-18 | 云南农业大学 | A kind of composition pesticide that can prevent and treat various plants disease fungus |
| CN112825915A (en) * | 2021-01-21 | 2021-05-25 | 中国科学院植物研究所 | Application of 1-octen-3-ol in preventing and treating postharvest diseases of fruits |
| CN113854292A (en) * | 2021-11-03 | 2021-12-31 | 上海市农业科学院 | Combined application of arginine and mushroom alcohol as deoxynivalenol synthesis inhibitor |
| CN113875754A (en) * | 2021-11-03 | 2022-01-04 | 上海市农业科学院 | Use of mushroom alcohol as biosynthesis inhibitor of three kinds of fusarium toxin |
| CN114342737A (en) * | 2022-01-26 | 2022-04-15 | 四川省食用菌研究所 | Paecilomyces varioti disease prevention and control method for morchella esculenta |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103429087A (en) * | 2011-03-11 | 2013-12-04 | 拜欧方吉特克有限责任公司 | Fungicidal and bactericidal composition that comprises volatile organic compounds of natural origin |
-
2015
- 2015-09-29 CN CN201510634635.1A patent/CN105145560A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103429087A (en) * | 2011-03-11 | 2013-12-04 | 拜欧方吉特克有限责任公司 | Fungicidal and bactericidal composition that comprises volatile organic compounds of natural origin |
Non-Patent Citations (1)
| Title |
|---|
| 苗建强等: "五种挥发性化合物对土传病原真菌及线虫的生物活性", 《植物保护学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109221110A (en) * | 2018-10-01 | 2019-01-18 | 云南农业大学 | A kind of composition pesticide that can prevent and treat various plants disease fungus |
| CN112825915A (en) * | 2021-01-21 | 2021-05-25 | 中国科学院植物研究所 | Application of 1-octen-3-ol in preventing and treating postharvest diseases of fruits |
| CN113854292A (en) * | 2021-11-03 | 2021-12-31 | 上海市农业科学院 | Combined application of arginine and mushroom alcohol as deoxynivalenol synthesis inhibitor |
| CN113875754A (en) * | 2021-11-03 | 2022-01-04 | 上海市农业科学院 | Use of mushroom alcohol as biosynthesis inhibitor of three kinds of fusarium toxin |
| CN114342737A (en) * | 2022-01-26 | 2022-04-15 | 四川省食用菌研究所 | Paecilomyces varioti disease prevention and control method for morchella esculenta |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| El Khetabi et al. | Role of plant extracts and essential oils in fighting against postharvest fruit pathogens and extending fruit shelf life: A review | |
| Dukare et al. | Non-fungicides-based promising technologies for managing post-production penicillium induced spoilage in horticultural commodities: a comprehensive review | |
| Tripathi et al. | Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables | |
| Venturini et al. | In vitro antifungal activity of several antimicrobial compounds against Penicillium expansum | |
| Wang et al. | Antifungal activities of neem (Azadirachta indica) seed kernel extracts on postharvest diseases in fruits | |
| US20040248764A1 (en) | Treatment and prevention of infections in plants | |
| CN102461653B (en) | Application of polyhexamethylene guanidine in citrus sour rot prevention and treatment, and citrus antistaling agent containing polyhexamethylene guanidine | |
| CN105145560A (en) | New application of 1-octene-3-ol as bactericide or bacteriostatic agent | |
| Zhang et al. | Inhibitory effect of chlorine dioxide (ClO2) fumigation on growth and patulin production and its mechanism in Penicillum expansum | |
| Saxena et al. | Marvellous Muscodor spp.: Update on their biology and applications | |
| CN105875592B (en) | One kind killing Neurospora composite drug | |
| Sehsah et al. | Efficacy of Bacillus subtilis, Moringa oleifera seeds extract and potassium bicarbonate on Cercospora leaf spot on sugar beet | |
| WO2000049865A2 (en) | Terpene compositions and methods of use | |
| Thanaboripat et al. | Inhibition of aflatoxin production and growth of Aspergillus flavus by citronella oil | |
| KR20090127498A (en) | Microbicidal and insecticidal composition for preventing plant pest and plant disease containing microorganisms and natural extracts | |
| Akhtar et al. | Evaluation of fungicides and biopesticides for the control of fusarium wiltof tomato | |
| Khadiri et al. | Challenges in apple preservation: Fungicide resistance and emerging biocontrols | |
| KR101143033B1 (en) | Compositions for protecting Plant diseases | |
| KR20160043547A (en) | Natural disinfection for horticultural crops produced thereby and method of manufacturing pesticides and natural disinfection and pesticides | |
| Mekonnen et al. | Antifungal activities of some essential oils against Fusarium oxysporum of rosemary and sage plants | |
| Abd-Alla et al. | Effect of some volatile compounds on black mould disease on onion bulbs during storage | |
| Şehirli et al. | The effect of acetic, formic and propionic acids on plant pathogenic fungi | |
| Ata et al. | In vitro Evaluation of Cymbopogon nardus essential oil against leaf disease fungus of narra (Pterocarpus indicus Wild) | |
| CN1275527C (en) | Plant source pesticide and production method thereof | |
| Suneeta et al. | Study of Antimicrobial Compounds of Bacillus subtilis (PSB5) and its Interaction with Fungicides against Fusarium oxysporum f. sp. gerberae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160614 Address after: 610000 No. 106 lion mountain road, Chengdu, Sichuan, Jinjiang District Applicant after: Institute of Nuclear and Biotechnology, Sichuan Academy of Agriculture Science Address before: 610066, Chengdu, Sichuan, Jinjiang District Province, No. 20 East Temple Road Applicant before: Sichuan Academy of Agricultural Sciences |
|
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151216 |
|
| RJ01 | Rejection of invention patent application after publication |