CN105145255B - A kind of apple dwarf is from root anvil seedling-wood breeding method - Google Patents
A kind of apple dwarf is from root anvil seedling-wood breeding method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of apple dwarf from root anvil seedling-wood breeding method, by the nursery of base anvil, zanjon nursery, autumn connect short anvil, cut anvil bud picking, carve wound smear medicine, bury dry promoting root growth, summer connect kind, secondary cut anvil bud picking, lifting cut anvil, classification storage etc. series of steps realize breeding from root anvil nursery stock, simplify Dwarf rootstocks nursery stock and breed step, improve breeding coefficient.
Description
Technical field
The present invention relates to apple nursery stock raising technology field, specially a kind of apple dwarf is from root anvil seedling-wood breeding method.
Background technology
Short anvil apple intensive cultivating system is not only the trend and direction of world's apple industry, and even more China is modern
The important symbol of apple industry, dwarfing rootstock is the main approach for realizing Apple Dwarf Stocks cultivation.Current world apple master
Produce state's Newly built orchard and substantially use short anvil intensive culture, most countries have accounted for more than the 90% of the apple gross area.And I
The existing apple orchard more than 90% of state is tall anvil fruit tree, and orchard closing, illumination is bad, and management is taken a lot of work, and is consumption high, a low yield
The traditional cultivation pattern for going out.
Carrying out short anvil apple intensive cultivating needs to cultivate substantial amounts of short anvil apple nursery stock, current apple dwarf seedling raising mannerses
There is intermediate stock nursery and from 2 kinds of root anvil nursery.
First, Dwarf rootstocks seedling is, with some good characteristics, particularly excellent Qiao to be showed aborning from root anvil seedling
Change anvil or dwarfing stock to cultivate by asexual reproduction methods such as tissue cultures, cuttage, press strips is stock, the grafting product on this stock
Kind, the nursery stock cultivated.This nursery stock is kind grafting on the stock of vegetative propagation, directly using the root system of stock, only
Grafting once, can overcome many shortcomings of seedling rootstock nursery stock, and the characteristics of have its own, developed country is widely used to educate from root anvil
Seedling, this method has the following advantages that：
（1）The merit apple for keeping maternal plant is generally vegetative propagation from the dwarfing stock of root anvil nursery stock, not by subtrahend
Division and the combination of property cell, have only carried out simple mitosis, it is to avoid miscellaneous and hereditary unstability.Therefore, formed
The characteristic of new individual is just the same with its maternal plant characteristic, still remains the intrinsic hereditary capacity of maternal plant, and can for a long time keep this
Merit.
（2）Regularity is high relatively to be concentrated from the distribution of root anvil height of tree degree, and change is significantly less than intermediate stock tree, orchard garden looks outward appearance
Neat apple intermediate stock nursery occupies leading position in China's apple nursery stock production.
（3）Fibrous root is more, and well developed root system is used as the germplasm of own-rooted tree stock, and its stem section or root system belong to stem source root or tiller root,
Power of regeneration is stronger, does not have obvious main root, and fibrous root is flourishing.
（4）Early bearing, high yield, own-rooted tree of fine quality are larger due to physiological age, it is not necessary to by from children to old ripe mistake
Journey, easily into flower.
Mainly have from the raising technology of root anvil nursery stock at present：
1st, micropropagation is to be bred using tissue culture technique, it is necessary to the support of certain instrument and equipment, technology etc..
2nd, mound layering has the methods such as mound layering, horizontal press strip.
3rd, cutting propagation is divided into the slotting method of root and stiff wood inserts method, is breeding from the basis of root anvil nursery stock in Grafted Variety
Formed with Dwarf rootstocks as stock, kind is two composition nursery stocks of scion.
Because China dwarfing rootstock resource is introduced from foreign countries mostly, the propagation nursery area set up at home after introduction compared with
It is small.And the breeding of most of dwarfing stock is more difficult, the support of certain instrument and equipment, technology is needed using tissue cultures mode,
It is unfavorable for the application and popularization of large area.Excellent dwarfing rootstock breeds relatively difficult by way of cuttage mostly, so I
State and foreign countries mound layering is used from breeding for root anvil mostly.
2nd, this short anvil seedling of the short anvil intermediate stock of invention of gardening scholar creativeness of dwarfing interstock China older generation
Wooden mode of reproduction, accelerates the utilization process of dwarfing stock.Dwarfing interstock seedling apple seedling is the reality bred with Malus spectabilis or crabapple
Used as base anvil, grafting dwarfing stock controls Growth status, then the grafting apple variety on dwarfing stock to raw seedling on base anvil, forms base
Three composition nursery stocks of anvil+dwarfing interstock+kind.
Although by cultivation practices for many years showing that using dwarfing interstock nursery stock building well dwarfing tree body can be reached, doing sth. in advance
The effect of result, but there is also big bound feet phenomenon more prominent, tree body early ageing phenomenon and add than more serious, tree body trunk disease
The disadvantage such as again.So Comparatively speaking the general performance of Dwarf rootstocks nursery stock building well is better than dwarfing interstock nursery stock.
The developing direction that intensive cultivating is China's Apple Industry is carried out using short anvil apple nursery stock building well.And short anvil apple
Dwarf rootstocks nursery stock should be widelyd popularize and applied in nursery stock.Because Dwarf rootstocks nursery stock is present：From root anvil seedling-wood breeding
Step is relatively complicated, the shortcomings of breeding coefficient is too low, so China is too small from the amount of root anvil nursery stock, far can not meet market
Demand, and the price serious popularization for limiting Dwarf rootstocks nursery stock too high.
How to simplify the propagation steps of Dwarf rootstocks nursery stock, improve breeding coefficient to reduce the breeding hardly possible from root anvil nursery stock
Degree, increases the amount from root anvil nursery stock, and it is to carry out intensive cultivation using Dwarf rootstocks nursery stock building well to reduce from the price of root anvil nursery stock
Whether training can be able to the key for quickly propelling.
The content of the invention
The present invention is directed to above weak point, there is provided a kind of apple dwarf is simplified short from root anvil seedling-wood breeding method
Change the propagation steps from root anvil nursery stock, improve breeding coefficient to reduce the breeding difficulty from root anvil nursery stock, increased from root anvil
The amount of nursery stock, reduces the price from root anvil nursery stock, has quickly propelled Dwarf rootstocks nursery stock building well and intensive culture.
The technical solution adopted for the present invention to solve the technical problems is：A kind of apple dwarf is from root anvil seedling-wood breeding side
Method, comprising having the following steps：
The first step：Base anvil nursery, this step provides material for second step,
Whole drill seeding kind at the beginning of 3 months, before sowing, nursery lot is ploughed deeply, soil deeptillage, is flattened and ditched in bedding, furrow and pour water in right amount,
Sowed after oozing under water, raked after broadcasting, covering with plastic film moisturizing is heated；Seeding method can select 70~80cm of wide row, narrow row
The double rows line seeding of 40cm, depth of planting is advisable with 2~3 times of seed diameter；
Second step：Base anvil is colonized, and this step provides material for the 3rd step,
Build garden land used turned over, sterilized, site preparation and fertilising, turn over after autumn, depth is 30--40cm, per 667m2Apply
High-quality compost 5000--10000kg, phosphate fertilizer 100kg, with mechanical whole furrow, ditching, furrow 1.5m wide is colonized ditch 20cm depths.At the beginning of 4 months
Zanjon nursery, when the base anvil seedling that the first step is cultivated grows 2~3 true leaves, by seedling transplant planting in field planting ditch, final singling
15~20 centimetres of spacing in the rows, mu stays ten thousand plants of seedling amount 0.9--1.2；
3rd step：Autumn connects short anvil, and this step provides material for the 4th step,
Mid or late August collection dwarfing stock kind health no disease and pests harm branch works as scion, and the base anvil young plant that second step is cultivated makees
It is stock, at base anvil young plant root neck position above 5cm place, is connect using the dwarfing stock kind that mode grafting gathers is connect with wooden bud
Fringe；
4th step：Cut anvil bud picking, this step provides material for the 5th step,
Second Year March, in the local cut anvil of autumn last year graft union above 0.5-1cm, top is all cut, after cut anvil
The rudiment of stock base portion is all erased, the young sprout for only staying the short anvil bud of grafting to sprout；
5th step：Carve wound and smear medicine, this step provides material for the 6th step,
Second Year mid-April when short anvil rudiment young sprout grows to 40cm or so, in the range of short anvil graft union above 20cm
Carried out carving 6 roads of wound with pocket knife, hinder standard at quarter：As deep as xylem, the 1/3-1/2 of dry crude degree based on length carved and use after wound
200ppmIBA smears wound；
6th step：Dry promoting root growth is buried, this step is that the supplement of the 7th step is consolidated,
Carve after wound smears medicine, the micro-ridge soil formed when second step is opened into zanjon is pushed aside to cover short anvil young sprout, carries out promoting root growth；
7th step：Summer connects kind, and this step provides material for the 8th step,
Second Year gathered at the beginning of 6 months need Grafted Variety health no disease and pests harm branch work as scion, with preceding step divide breed it is short
Anvil does stock, and in stock, 20-30cm is local from the ground, and mode grafting improved seeds are connect using with wooden bud；
8th step：Secondary cut anvil bud picking, this step provides material for the 9th step,
The one week after of grafting carries out cut anvil and promotees to sprout in grafting interface above 0.5-1cm scopes, more than grafting interface
The scion of 0.5-1cm is all cut, and the rudiment beyond Grafted Variety bud is all erased, and is only retained Grafted Variety bud and is sprouted what is produced
Young sprout；
9th step：Lifting cut anvil, the step of this step the ten provides material,
Arrive by the end of October it is freeze-up before, nursery stock is dug out, the base anvil below graft union when the 3rd step autumn was connect into dwarfing stock is cut
Remove；
Tenth step：Classification storage, this step is this patent final step,
The nursery stock that the treatment of the 9th step will be carried out is classified according to standards such as rugosity, height, and the nursery stock of point good level is carried out
Heel in storage
The beneficial effects of the invention are as follows：
Second step：Zanjon nursery changes traditional flat furrow nursery as one of this patent core procedure, and small seed is existed
In zanjon, its Main Function is：It is the 6th step that zanjon nursery is easy to the fertilizing, watering in seedling raising process to operate what is more important
Bury dry promoting root growth operation and facility is provided.
3rd step：Autumn connects and most importantly change in short anvil grafting part for 5cm from the ground, and such 6th step buries dry promoting root growth
More dwarfing stocks can be buried in the earth so as to promoting root growth.
5th step：Carve wound and smear medicine one of for this patent core procedure, carve wound, smear two operations of medicine dwarfing stock can be promoted to exist
More adventitious roots can be sent during the treatment of the 6th step.
6th step：It is one of this patent core procedure to bury dry promoting root growth.The restrictive factor bred of Dwarf rootstocks nursery stock
It is that the numerous coefficient of expansion is low.Generally conventional press strip mode is general to want the branch of 40-50cm just to breed 2-3 nursery stock, one mu
The dwarfing stock female parent garden on ground is at best able to breed ten thousand plants of 2-3 from root anvil nursery stock；But dwarfing stock can be promoted to connect by this step
Bud sprouting branch buries native part and goes out adventitious root so that breeds a dwarfing stock seedling from a dwarfing stock bud and is possibly realized,
Significantly improve breeding coefficient so that the dwarfing stock of 0.07 hectare female parent garden can breed 10-15 ten thousand from root anvil seedling.
9th step：Lifting cut anvil is one of this patent core procedure, and it is two compositions to change three compositions by this step,
It is from root anvil that realization changes intermediate stock.
Brief description of the drawings
It is operational flowchart of the invention shown in Fig. 1.
Specific embodiment
The present invention will be described in detail with specific embodiment below in conjunction with the accompanying drawings：
Embodiment one：Rich No. 3 Dwarf rootstocks seedling of cigarette is bred using this patent method.
Material：Seed：Hupehensis Rehd；Dwarfing stock：M9T337, kind：Cigarette is rich No. 3.
Time：- 2013 years 2012.
Process：Whole drill seeding kind at the beginning of 3 months 2012, before sowing, nursery lot is ploughed deeply, soil deeptillage, flattens trench digging in bedding, furrow
And pour water in right amount, sowed after oozing under water, to be raked after broadcasting, covering with plastic film moisturizing is heated；Seeding method can select wide row 70~
The double rows line seeding of 80cm, narrow row 40cm, hupehensis Rehd depth of planting is advisable with 2~3 times of seed diameter；Building garden land used is carried out
Turn over, sterilize, site preparation and fertilising, turn over after autumn, depth is 30--40cm, per 667m2Apply high-quality compost 5000--
10000kg, phosphate fertilizer 100kg, with mechanical whole furrow, ditching, furrow 1.5m wide is colonized ditch 20cm depths.Zanjon nursery at the beginning of 4 months, when base anvil
When seedling grows 2~3 true leaves, by seedling transplant planting in field planting ditch, 15~20 centimetres of final singling spacing in the rows, mu stays seedling amount
Ten thousand plants of 0.9--1.2；Mid or late August collection M9T337 dwarfing stocks kind health no disease and pests harm branch works as scion, the base anvil of cultivation
Young plant at base anvil young plant root neck above 5cm, the dwarfing stock product that mode grafting is gathered is connect using with wooden bud as stock
Plant scion；Second Year March, in the local cut anvil of autumn last year graft union above 0.5-1cm, top is all cut, will after cut anvil
The rudiment of stock base portion is all erased, the young sprout for only staying the short anvil bud of grafting to sprout；Second Year works as short anvil rudiment young sprout mid-April
When growing to 40cm or so, carried out carving 6 roads of wound with pocket knife in the range of short anvil graft union above 20cm, hinder standard at quarter：As deep as wooden
Portion, the 1/3-1/2 of dry crude degree based on length to be carved and smear wound with 200ppmIBA after wound；Carve after wound smears medicine, second step is opened
The micro-ridge soil formed during zanjon is pushed aside to cover short anvil young sprout, carries out promoting root growth；Second Year is gathered at the beginning of 6 months and needs Grafted Variety cigarette richness 3
Number healthy no disease and pests harm branch work as scion, stock is done with the short anvil that preceding step point is bred, in stock 20-30cm from the ground
Side, mode grafting improved seeds are connect using with wooden bud；The one week after of grafting is cut in grafting interface above 0.5-1cm scopes
Anvil promotees to sprout, and the scion of grafting interface above 0.5-1cm is all cut, and the rudiment beyond Grafted Variety bud is all erased, only
Retain Grafted Variety bud and sprout the young sprout for producing；Arrive by the end of October it is freeze-up before, nursery stock is dug out, when the 3rd step autumn was connect into dwarfing stock
Graft union below base anvil wipe out；The nursery stock that the treatment of the 9th step will be carried out is classified according to standards such as rugosity, height, will be divided
The nursery stock of good level carries out heeling in storage
Embodiment two：The Dwarf rootstocks seedling of the richest No. 1 is bred using this patent method.
Material：Seed：Hupehensis Rehd；Dwarfing stock：M9T337, kind：The richest No. 1.
Time：- 2013 years 2012.
Process：Whole drill seeding kind at the beginning of 3 months 2012, before sowing, nursery lot is ploughed deeply, soil deeptillage, flattens trench digging in bedding, furrow
And pour water in right amount, sowed after oozing under water, to be raked after broadcasting, covering with plastic film moisturizing is heated；Seeding method can select wide row 70~
The double rows line seeding of 80cm, narrow row 40cm, hupehensis Rehd depth of planting is advisable with 2~3 times of seed diameter；Building garden land used is carried out
Turn over, sterilize, site preparation and fertilising, turn over after autumn, depth is 30--40cm, per 667m2Apply high-quality compost 5000--
10000kg, phosphate fertilizer 100kg, with mechanical whole furrow, ditching, furrow 1.5m wide is colonized ditch 20cm depths.Zanjon nursery at the beginning of 4 months, when base anvil
When seedling grows 2~3 true leaves, by seedling transplant planting in field planting ditch, 15~20 centimetres of final singling spacing in the rows, mu stays seedling amount
Ten thousand plants of 0.9--1.2；Mid or late August collection M9T337 dwarfing stocks kind health no disease and pests harm branch works as scion, the base anvil of cultivation
Young plant at base anvil young plant root neck above 5cm, the dwarfing stock product that mode grafting is gathered is connect using with wooden bud as stock
Plant scion；Second Year March, in the local cut anvil of autumn last year graft union above 0.5-1cm, top is all cut, will after cut anvil
The rudiment of stock base portion is all erased, the young sprout for only staying the short anvil bud of grafting to sprout；Second Year works as short anvil rudiment young sprout mid-April
When growing to 40cm or so, carried out carving 6 roads of wound with pocket knife in the range of short anvil graft union above 20cm, hinder standard at quarter：As deep as wooden
Portion, the 1/3-1/2 of dry crude degree based on length to be carved and smear wound with 200ppmIBA after wound；Carve after wound smears medicine, second step is opened
The micro-ridge soil formed during zanjon is pushed aside to cover short anvil young sprout, carries out promoting root growth；Second Year is gathered at the beginning of 6 months and needs the Grafted Variety richest 1
Number healthy no disease and pests harm branch work as scion, stock is done with the short anvil that preceding step point is bred, in stock 20-30cm from the ground
Side, mode grafting improved seeds are connect using with wooden bud；The one week after of grafting is cut in grafting interface above 0.5-1cm scopes
Anvil promotees to sprout, and the scion of grafting interface above 0.5-1cm is all cut, and the rudiment beyond Grafted Variety bud is all erased, only
Retain Grafted Variety bud and sprout the young sprout for producing；Arrive by the end of October it is freeze-up before, nursery stock is dug out, when the 3rd step autumn was connect into dwarfing stock
Graft union below base anvil wipe out；The nursery stock that the treatment of the 9th step will be carried out is classified according to standards such as rugosity, height, will be divided
The nursery stock of good level carries out heeling in storage
Embodiment three：The Dwarf rootstocks seedling of loud, high-pitched sound is bred using this patent method.
Material：Seed：Hupehensis Rehd；Dwarfing stock：M9, kind：Loud, high-pitched sound.
Time：- 2014 years 2013.
Process：Whole drill seeding kind at the beginning of 3 months 2012, before sowing, nursery lot is ploughed deeply, soil deeptillage, flattens trench digging in bedding, furrow
And pour water in right amount, sowed after oozing under water, to be raked after broadcasting, covering with plastic film moisturizing is heated；Seeding method can select wide row 70~
The double rows line seeding of 80cm, narrow row 40cm, hupehensis Rehd depth of planting is advisable with 2~3 times of seed diameter；Building garden land used is carried out
Turn over, sterilize, site preparation and fertilising, turn over after autumn, depth is 30--40cm, per 667m2Apply high-quality compost 5000--
10000kg, phosphate fertilizer 100kg, with mechanical whole furrow, ditching, furrow 1.5m wide is colonized ditch 20cm depths.Zanjon nursery at the beginning of 4 months, when base anvil
When seedling grows 2~3 true leaves, by seedling transplant planting in field planting ditch, 15~20 centimetres of final singling spacing in the rows, mu stays seedling amount
Ten thousand plants of 0.9--1.2；Mid or late August collection M9 dwarfing stocks kind health no disease and pests harm branch works as scion, the base anvil young plant of cultivation
As stock, at base anvil young plant root neck position above 5cm places, connect using the dwarfing stock kind that mode grafting gathers is connect with wooden bud
Fringe；Second Year March, in the local cut anvil of autumn last year graft union above 0.5-1cm, top is all cut, by stock after cut anvil
The rudiment of base portion is all erased, the young sprout for only staying the short anvil bud of grafting to sprout；Second Year mid-April grows to when short anvil rudiment young sprout
During 40cm or so, carried out carving 6 roads of wound with pocket knife in the range of short anvil graft union above 20cm, hinder standard at quarter：It is long as deep as xylem
The 1/3-1/2 of dry crude degree based on degree, to carve and smear wound with 200ppmIBA after hindering；Carve after wound smears medicine, when second step is opened into zanjon
The micro-ridge soil of formation is pushed aside to cover short anvil young sprout, carries out promoting root growth；Second Year is gathered at the beginning of 6 months needs Grafted Variety loud, high-pitched sound health disease-free
Insect pest branch works as scion, and stock is done with the short anvil that preceding step point is bred, and in stock, 20-30cm is local from the ground, using band wood
Matter bud connects mode grafting improved seeds；The one week after of grafting carries out cut anvil and promotees to sprout in grafting interface above 0.5-1cm scopes, will
The scion of grafting interface above 0.5-1cm is all cut, and the rudiment beyond Grafted Variety bud is all erased, and only retains Grafted Variety
Bud sprouts the young sprout for producing；Arrive by the end of October it is freeze-up before, nursery stock is dug out, below graft union when the 3rd step autumn to be connect dwarfing stock
Base anvil wipe out；The nursery stock that the treatment of the 9th step will be carried out is classified according to standards such as rugosity, height, and the nursery stock of point good level is entered
Row heels in storage
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
Change, remodeling, addition or replacement that the technical staff in domain is made in essential scope of the invention, should also belong to of the invention
Protection domain.
Claims (1)
1. a kind of apple dwarf is from root anvil seedling-wood breeding method, it is characterised in that comprising having the following steps：
The first step：Base anvil nursery, this step provides material for second step,
Whole drill seeding kind at the beginning of 3 months, before sowing, nursery lot is ploughed deeply, soil deeptillage, is flattened and ditched in bedding, furrow and pour water in right amount, treats water
Under ooze after sow, raked after broadcasting, covering with plastic film moisturizing is heated；Seeding method from 70~80cm of wide row, narrow row 40cm it is double
Row drilling, depth of planting is advisable with 2~3 times of seed diameter；
Second step：Zanjon nursery, this step provides material for the 3rd step,
Build garden land used turned over, sterilized, site preparation and fertilising, turn over after autumn, depth is 30-40cm, per 667m2Apply high-quality soil
Excrement 5000-10000kg, phosphate fertilizer 100kg, with mechanical whole furrow, ditching, furrow 1.5m wide is colonized ditch 20cm depths, and zanjon at the beginning of 4 months is educated
Seedling, when the base anvil seedling that the first step is cultivated grows 2~3 true leaves, by seedling transplant planting in field planting ditch, final singling spacing in the rows
15~20 centimetres, mu stays ten thousand plants of seedling amount 0.9-1.2；
3rd step：Autumn connects short anvil, and this step provides material for the 4th step,
Mid or late August collection dwarfing stock kind health no disease and pests harm branch works as scion, and the base anvil young plant that second step is cultivated is used as anvil
Wood, at base anvil young plant root neck above 5cm, the dwarfing stock kind scion that mode grafting is gathered is connect using with wooden bud；
4th step：Cut anvil bud picking, this step provides material for the 5th step,
Second Year March, in the local cut anvil of autumn last year graft union above 0.5-1cm, top is all cut, by anvil after cut anvil
The rudiment of wooden base portion is all erased, the young sprout for only staying the short anvil bud of grafting to sprout；
5th step：Carve wound and smear medicine, this step provides material for the 6th step,
Second Year mid-April when short anvil rudiment young sprout grows to 40cm or so, with small in the range of short anvil graft union above 20cm
Knife carries out carving 6 roads of wound, hinders standard at quarter：As deep as xylem, the 1/3-1/2 of dry crude degree based on length to be carved and use 200ppmIBA after wound
Smear wound；
6th step：Dry promoting root growth is buried, this step is that the supplement of the 7th step is consolidated,
Carve after wound smears medicine, the micro-ridge soil formed when second step is opened into zanjon is pushed aside to cover short anvil branch, carries out promoting root growth；
7th step：Summer connects kind, and this step provides material for the 8th step,
Second Year is gathered at the beginning of 6 months needs Grafted Variety health no disease and pests harm branch to work as scion, and anvil is done with the short anvil that preceding step is bred
Wood, in stock, 20-30cm is local from the ground, and mode grafting improved seeds are connect using with wooden bud；
8th step：Secondary cut anvil bud picking, this step provides material for the 9th step,
The one week after of grafting carries out cut anvil and promotees to sprout in grafting interface above 0.5-1cm scopes, by grafting interface above 0.5-1cm
Scion all cut, the rudiment beyond Grafted Variety bud is all erased, only retain a Grafted Variety bud and sprout the young sprout for producing；
9th step：Lifting cut anvil, the step of this step the ten provides material,
Arrive by the end of October it is freeze-up before, nursery stock is dug out, the base anvil below graft union when the 3rd step autumn was connect into dwarfing stock is wiped out；
Tenth step：Classification storage, this step is this patent final step,
The nursery stock that the treatment of the 9th step will be carried out is classified according to rugosity, Height Standard, and the nursery stock of point good level is carried out heeling in storage
Hide.
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| CN107318475A (en) * | 2017-07-12 | 2017-11-07 | 北京市农林科学院 | A kind of apple is from root anvil with branch big seedling cultivation method |
| CN107173083A (en) * | 2017-07-17 | 2017-09-19 | 杨凌职业技术学院 | A kind of Apples Dwarf Stocks grafting seedlings-growing method |
| CN108541469B (en) * | 2018-03-19 | 2020-06-16 | 山东省果树研究所 | Winter grafting rapid seedling method for dwarf apple interstock |
| CN113068562A (en) * | 2018-08-02 | 2021-07-06 | 辽宁省果树科学研究所 | Grafting method for dwarf apples |
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