CN105123298B - A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm - Google Patents
A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm Download PDFInfo
- Publication number
- CN105123298B CN105123298B CN201510396945.4A CN201510396945A CN105123298B CN 105123298 B CN105123298 B CN 105123298B CN 201510396945 A CN201510396945 A CN 201510396945A CN 105123298 B CN105123298 B CN 105123298B
- Authority
- CN
- China
- Prior art keywords
- beanpod
- eating
- bean worm
- core bean
- blackhead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 44
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 44
- 244000068988 Glycine max Species 0.000 title claims abstract description 42
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 42
- 238000004166 bioassay Methods 0.000 title claims abstract description 19
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 41
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 41
- 210000004681 ovum Anatomy 0.000 claims abstract description 41
- 206010000496 acne Diseases 0.000 claims abstract description 30
- 241000238631 Hexapoda Species 0.000 claims abstract description 20
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 6
- 238000011835 investigation Methods 0.000 claims abstract description 6
- 235000013601 eggs Nutrition 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 11
- 230000003020 moisturizing effect Effects 0.000 claims description 10
- 230000012447 hatching Effects 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 6
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 5
- 240000001624 Espostoa lanata Species 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 11
- 238000012797 qualification Methods 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000003556 assay Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Catching Or Destruction (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm, belong to soybean insect resistance capacity indoor appraising method technical field.Described assay method comprises the following steps：Pairing eating-core bean worm adult is accessed into beanpod, constant-temperature moisture-keeping in constant-temperature shaking incubator is put into and is incubated the eating-core bean worm ovum grain obtained with blackhead；The beanpod pod wall of the eating-core bean worm ovum grain with blackhead of culture is cut into pieces, each small pieces have 2 ovum grains with blackhead, it is inoculated with the non-selective experiment of newly hatched larvae, the eating-core bean worm work borer population that culture of pests is survived on the 6th~8 day in investigation beanpod one by one using each beanpod.The method of the present invention reduces the error of Soybean Resistance heart-eating worm indoor biometricses qualification result, it is inoculated with the non-selective experiment of newly hatched larvae one by one using each beanpod, ensure there is newly hatched larvae on each beanpod, the contact probability of beanpod and newly hatched larvae is allowed to be consistent, so as to improve the accuracy of qualification result.
Description
Technical field
The present invention relates to a kind of soybean insect resistance capacity indoor appraising method, more particularly to a kind of non-selective identification Soybean Resistance
The indoor bioassay method of eating-core bean worm.
Background technology
Current Soybean Resistance heart-eating worm indoor bioassay is main to be equal to design in 2011 by Northeast Agricultural University's Han Lan hazes
Disk split plot design (the such as Li Tingting, Han Lanlan, Zhao Kuijun " application of the disk split plot design on genetically engineered soybean insect resistance identification "《Make
Thing magazine》2011,4:20-22).This method is under the suitable temperature and humidity conditions of insect growth, by being placed on circular filter paper
The newly hatched larvae at center equidistantly randomly places the trend and moth food, death condition of beanpod to disk periphery to judge that insect resistace is poor
It is different.Disk split plot design identifies that soybean is a kind of selectivity experiment to eating-core bean worm resistance capacity, but eating-core bean worm is to posting
Main selection is that occur to carry out the beanpod of host selection spawning in Adult worms producting eggs stage, i.e. adult, newly hatched larvae directly eat into
Place beanpod, rather than larva are selected beanpod.This method allows newly hatched larvae independently to randomly choose beanpod, it is impossible to meet every
There is newly hatched larvae on individual beanpod.So the probability that beanpod is contacted with newly hatched larvae is inconsistent, the qualification result thus increased
Error.
The content of the invention
It is an object of the invention to overcome shortcoming present in above-mentioned prior art with deficiency, there is provided the non-selective mirror of one kind
Determine the indoor bioassay method of Soybean Resistance eating-core bean worm.This method is tied to reduce the identification of Soybean Resistance heart-eating worm indoor biometricses
The error of fruit, the non-selective experiment of newly hatched larvae is inoculated with using each beanpod one by one, it is ensured that is had on each beanpod and is just incubated children
Worm, allows the contact probability of beanpod and newly hatched larvae to be consistent, so as to improve the accuracy of qualification result.
The purpose of the present invention is achieved through the following technical solutions：A kind of room of non-selective identification Soybean Resistance eating-core bean worm
Interior bioassay method, comprises the following steps：
(1) prepare for examination insect：The adult production for being connected to beanpod is put into after the eating-core bean worm adult pairing that field is gathered
In ovum cage, each spawning cage is put into 40~50 pairs of pairing eating-core bean worm adults, and fresh beanpod is changed daily and supplies Adult worms producting eggs, will
Beanpod with ovum is put into constant-temperature moisture-keeping in constant incubator and is incubated, and treats that egg hatching checks the development progress of ovum daily, when ovum grain
Occur after blackhead, obtain the eating-core bean worm ovum grain beanpod with blackhead；
(2) preparation of soybean material：The consistent beanpod of growth is gathered in field, valve bag is put into and takes back, each culture dish
10 beanpods are put into, beanpod is closely spaced together, the base portion of beanpod is encased with moisturizing cotton balls, and moisturizing is stand-by, and preparation is obtained
The culture dish of soybean pod must be carried；
(3) the beanpod pod wall for the eating-core bean worm ovum grain with blackhead for obtaining step (1) is carefully cut, and knife is used after counting
Piece cuts the beanpod pod wall of the eating-core bean worm ovum grain with blackhead into pieces, and each small pieces have 2 ovum grains with blackhead, use tweezers
Small pieces are transferred in step (2) in the culture dish with soybean pod, there are 2 bands to each beanpod access is a piece of in culture dish
The pod wall of the ovum grain of blackhead, is put into insectary and cultivates by 20 altogether, and pod is voluntarily eaten into after it is hatched；
(4) culture of pests pushes the eating-core bean worm work borer population survived in beanpod investigation beanpod aside after 6~8 days, realizes non-
The indoor bioassay of selectivity identification soybean insect resistace.
The temperature that constant-temperature moisture-keeping described in step (1) is incubated is 25 DEG C, and relative humidity is 80%.
Counting described in step (3) is preferably to be counted under stereoscope.
The condition cultivated in culturing room described in step (3) is that temperature is 25 DEG C, and relative humidity is 80%.
The present invention has the following advantages and effect relative to prior art：
The method of the present invention reduces the error of Soybean Resistance heart-eating worm indoor biometricses qualification result, is connect one by one using each beanpod
Plant the non-selective experiment of newly hatched larvae, it is ensured that have newly hatched larvae on each beanpod, make contact of the beanpod with newly hatched larvae several
Rate is consistent, so as to improve the accuracy of qualification result.
Embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
Embodiment 1
The invention provides a kind of indoor bioassay method of non-selective identification soybean insect resistace, including following step
Suddenly：
(1) prepare for examination insect：Be put into after the eating-core bean worm adult pairing that field is gathered access beanpod in advance into
In worm spawning cage, each spawning cage is put into 40 pairs of pairing eating-core bean worm adults, and fresh beanpod is changed daily and supplies Adult worms producting eggs, will
Beanpod with ovum is put into constant-temperature moisture-keeping incubation in constant incubator and treats egg hatching (25 DEG C of temperature, relative humidity 80%), inspection daily
The development progress of ovum is looked into, when ovum grain occurs taking out standby after blackhead；
(2) preparation of soybean material：Gathered in field after soybean material grows consistent beanpod, numbering and be put into valve bag band
Laboratory is gone back to, the beanpod in 1 Ge Long areas is put into 1 glass culture dish, and each culture dish is put into 10 beanpods, and beanpod is close
Arranged together, the base portion of beanpod is encased with moisturizing cotton balls, and moisturizing is stand-by；
(3) test process：The beanpod pod wall for the eating-core bean worm ovum grain with blackhead that step (1) is obtained carefully is cut,
The beanpod pod wall of the eating-core bean worm ovum grain with blackhead is cut into pieces with blade after counting, each small pieces there are 2 with blackhead
Small pieces, are transferred in step (2) in the culture dish with soybean pod by ovum grain with tweezers, and each beanpod in culture dish is accessed
It is a piece of have 2 with blackhead ovum grain pod wall, 20 altogether, be put into insectary cultivate, after its hatching after voluntarily eat into pod, support worm
The condition of culture of room is that temperature is 25 DEG C, and relative humidity is 80%；
(4) investigate：Culture of pests pushes the eating-core bean worm work borer population survived in beanpod investigation beanpod aside after 6 days, realize
The indoor bioassay of non-selective identification soybean insect resistace.
Embodiment 2
The invention provides a kind of indoor bioassay method of non-selective identification soybean insect resistace, including following step
Suddenly：
(1) prepare for examination insect：Be put into after the eating-core bean worm adult pairing that field is gathered access beanpod in advance into
In worm spawning cage, each spawning cage is put into 45 pairs of pairing eating-core bean worm adults, and fresh beanpod is changed daily and supplies Adult worms producting eggs, will
Beanpod with ovum is put into constant-temperature moisture-keeping incubation in constant incubator and treats egg hatching (25 DEG C of temperature, relative humidity 80%), inspection daily
The development progress of ovum is looked into, when ovum grain occurs taking out standby after blackhead；
(2) preparation of soybean material：Gathered in field after soybean material grows consistent beanpod, numbering and be put into valve bag band
Laboratory is gone back to, the beanpod in 1 Ge Long areas is put into 1 glass culture dish, and each culture dish is put into 10 beanpods, and beanpod is close
Arranged together, the base portion of beanpod is encased with moisturizing cotton balls, and moisturizing is stand-by；
(3) test process：The beanpod pod wall for the eating-core bean worm ovum grain with blackhead that step (1) is obtained carefully is cut,
The beanpod pod wall of the eating-core bean worm ovum grain with blackhead is cut into pieces with blade after counting, each small pieces there are 2 with blackhead
Small pieces, are transferred in step (2) in the culture dish with soybean pod by ovum grain with tweezers, and each beanpod in culture dish is accessed
It is a piece of have 2 with blackhead ovum grain pod wall, 20 altogether, be put into insectary cultivate, after its hatching after voluntarily eat into pod, support worm
The condition of culture of room is that temperature is 25 DEG C, and relative humidity is 80%；
(4) investigate：Culture of pests pushes the eating-core bean worm work borer population survived in beanpod investigation beanpod aside after 7 days, realize
The indoor bioassay of non-selective identification soybean insect resistace.
Embodiment 3
The invention provides a kind of indoor bioassay method of non-selective identification soybean insect resistace, including following step
Suddenly：
(1) prepare for examination insect：Be put into after the eating-core bean worm adult pairing that field is gathered access beanpod in advance into
In worm spawning cage, each spawning cage is put into 50 pairs of pairing eating-core bean worm adults, and fresh beanpod is changed daily and supplies Adult worms producting eggs, will
Beanpod with ovum is put into constant-temperature moisture-keeping incubation in constant incubator and treats egg hatching (25 DEG C of temperature, relative humidity 80%), inspection daily
The development progress of ovum is looked into, when ovum grain occurs taking out standby after blackhead；
(2) preparation of soybean material：Gathered in field after soybean material grows consistent beanpod, numbering and be put into valve bag band
Laboratory is gone back to, the beanpod in 1 Ge Long areas is put into 1 glass culture dish, and each culture dish is put into 10 beanpods, and beanpod is close
Arranged together, the base portion of beanpod is encased with moisturizing cotton balls, and moisturizing is stand-by；
(3) test process：The beanpod pod wall for the eating-core bean worm ovum grain with blackhead that step (1) is obtained carefully is cut,
The beanpod pod wall of the eating-core bean worm ovum grain with blackhead is cut into pieces with blade after counting, each small pieces there are 2 with blackhead
Small pieces, are transferred in step (2) in the culture dish with soybean pod by ovum grain with tweezers, and each beanpod in culture dish is accessed
It is a piece of have 2 with blackhead ovum grain pod wall, 20 altogether, be put into insectary cultivate, after its hatching after voluntarily eat into pod, support worm
The condition of culture of room is that temperature is 25 DEG C, and relative humidity is 80%；
(4) investigate：Culture of pests pushes the eating-core bean worm work borer population survived in beanpod investigation beanpod aside after 8 days, realize
The indoor bioassay of non-selective identification soybean insect resistace.
Data statistics and analysis：Duncan Multiple range tests are carried out using the single factor test function of statistic analysis of DPS softwares.
By taking AG serial soybean materials as an example, indoor anti-eating-core bean worm is carried out by this method and identified, as a result shows there is 8
The insect resistace of part material shows significant difference compared with acceptor control Willams82, as a result as shown in table 1.
The insect resistace identification of the AG series materials of table 1
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (4)
1. a kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm, it is characterised in that：Including following
Step：
(1) prepare for examination insect：The Adult worms producting eggs cage for being connected to beanpod is put into after the eating-core bean worm adult pairing that field is gathered
In, each spawning cage is put into 40~50 pairs of pairing eating-core bean worm adults, and fresh beanpod is changed daily and supplies Adult worms producting eggs, will be with ovum
Beanpod be put into constant incubator constant-temperature moisture-keeping and be incubated, treat that egg hatching checks the development progress of ovum daily, when ovum grain occurs
After blackhead, the eating-core bean worm ovum grain beanpod with blackhead is obtained；
(2) preparation of soybean material：The consistent beanpod of growth is gathered in field, valve bag is put into and takes back, each culture dish is put into
10 beanpods, beanpod is closely spaced together, and the base portion of beanpod is encased with moisturizing cotton balls, and moisturizing is stand-by, prepares band
There is the culture dish of soybean pod；
(3) the beanpod pod wall for the eating-core bean worm ovum grain with blackhead for obtaining step (1) is carefully cut, will with blade after counting
The beanpod pod wall of eating-core bean worm ovum grain with blackhead is cut into pieces, and each small pieces have 2 ovum grains with blackhead, will be small with tweezers
Piece is transferred in step (2) in the culture dish with soybean pod, and a piece of to each beanpod access in culture dish have 2 band blackheads
Ovum grain pod wall, 20 altogether, be put into insectary cultivate, after its hatching after voluntarily eat into pod；
(4) culture of pests pushes the eating-core bean worm work borer population survived in beanpod investigation beanpod aside after 6~8 days, realizes non-selection
Property identification soybean insect resistace indoor bioassay.
2. the indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm according to claim 1, it is special
Levy and be：The temperature that constant-temperature moisture-keeping described in step (1) is incubated is 25 DEG C, and relative humidity is 80%.
3. the indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm according to claim 1, it is special
Levy and be：Being counted as described in step (3) counts under stereoscope.
4. the indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm according to claim 1, it is special
Levy and be：The condition cultivated in culturing room described in step (3) is that temperature is 25 DEG C, and relative humidity is 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510396945.4A CN105123298B (en) | 2015-07-08 | 2015-07-08 | A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510396945.4A CN105123298B (en) | 2015-07-08 | 2015-07-08 | A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105123298A CN105123298A (en) | 2015-12-09 |
| CN105123298B true CN105123298B (en) | 2017-07-11 |
Family
ID=54709196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510396945.4A Expired - Fee Related CN105123298B (en) | 2015-07-08 | 2015-07-08 | A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105123298B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11135806B2 (en) | 2018-02-16 | 2021-10-05 | American Nano Llc. | Compositions incorporating silica fibers |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1731512A1 (en) * | 2004-03-05 | 2006-12-13 | Nissan Chemical Industries, Ltd. | Isoxazoline-substituted benzamide compound and noxious organism control agent |
| CN101365795A (en) * | 2005-09-16 | 2009-02-11 | 德福根有限公司 | Dsrna used as pest control agent |
| CN101891710A (en) * | 2010-07-27 | 2010-11-24 | 南开大学 | 4-halogenated methyl-1,2,3-thiadiazole compounds and preparation method and application thereof |
| CN101917852A (en) * | 2007-10-04 | 2010-12-15 | 陶氏益农公司 | Pesticidal compositions |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9133475B2 (en) * | 2008-11-26 | 2015-09-15 | Board Of Trustees Of Michigan State University | Aphid resistant soybean plants |
-
2015
- 2015-07-08 CN CN201510396945.4A patent/CN105123298B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1731512A1 (en) * | 2004-03-05 | 2006-12-13 | Nissan Chemical Industries, Ltd. | Isoxazoline-substituted benzamide compound and noxious organism control agent |
| CN101365795A (en) * | 2005-09-16 | 2009-02-11 | 德福根有限公司 | Dsrna used as pest control agent |
| CN101917852A (en) * | 2007-10-04 | 2010-12-15 | 陶氏益农公司 | Pesticidal compositions |
| CN101891710A (en) * | 2010-07-27 | 2010-11-24 | 南开大学 | 4-halogenated methyl-1,2,3-thiadiazole compounds and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 棉花抗棉铃虫性室内生物测定新方法;马丽华等;《中国棉花》;19980720(第07期);第27页 * |
| 转Bt基因棉抗虫性的室内鉴定技术;周冬生等;《安徽农业科学》;20001025(第05期);第620-622页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105123298A (en) | 2015-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Khanamani et al. | Linking pollen quality and performance of Neoseiulus californicus (Acari: Phytoseiidae) in two‐spotted spider mite management programmes | |
| Blaauw et al. | Larger wildflower plantings increase natural enemy density, diversity, and biological control of sentinel prey, without increasing herbivore density | |
| CN109965088A (en) | Spodopterafrugiperda larva artificial feed, preparation method and application | |
| San Martin y Gomez et al. | Ecotypic differentiation between urban and rural populations of the grasshopper Chorthippus brunneus relative to climate and habitat fragmentation | |
| CN110367407A (en) | A kind of Spodopterafrugiperda larva artificial feed and its preparation method and application | |
| CN105766808B (en) | The method that compatriot likes raising and breeding in victory earthworm room | |
| CN105123298B (en) | A kind of indoor bioassay method of non-selective identification Soybean Resistance eating-core bean worm | |
| Pfitzer et al. | Life history traits and a method for continuous mass rearing of the planthopper Pentastiridius leporinus, a vector of the causal agent of syndrome ‘basses richesses’ in sugar beet | |
| Cunningham et al. | Reproduction in the Mediterranean fruit fly: depletion of stored sperm in females | |
| CN106106375A (en) | A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth | |
| Ye et al. | Population parameters and feeding preference of Spodoptera litura (Lepidoptera: Noctuidae) on different Asparagus officinalis tissues | |
| CN106416715B (en) | Method for identifying anti-selection performance of soybean seedling stage on prodenia litura larvae living bodies | |
| CN107279136B (en) | A kind of araecerus fasciculatus attractant and its application | |
| Hanife | Modified agar-based diet for small scale laboratory rearing of olive fruit fly, Bactrocera oleae (Diptera: Tephritidae) | |
| Kadkhodazadeh et al. | Suitability of different pollen grains and Tetranychus urticae as food for the predatory mite, Amblyseius swirskii (Acari: Phytoseiidae) | |
| Nandhini et al. | Host Plant Feeding and Ovipositional Preferences of Spodoptera frugiperda (Lepidoptera: Noctuidae) under Laboratory Conditions1 | |
| Yin et al. | Reduced plant nutrition under elevated CO2 depresses the immunocompetence of cotton bollworm against its endoparasite | |
| Marri et al. | Basic Developmental Characteristics of the Fall Armyworm, Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae), Reared under Laboratory Conditions | |
| Yeargan et al. | Comparison of common milkweed and honeyvine milkweed (Asclepiadaceae) as host plants for monarch larvae (Lepidoptera: Nymphalidae) | |
| Mo et al. | Interactive effect of diet and temperature on instar numbers in Spodoptera litura, with reference to head capsule width and weight | |
| Liao et al. | Temperature-dependent development and reproduction of rice leaffolder, Marasmia exigua (Lepidoptera: Pyralidae) | |
| Rizal et al. | Optimal mycelial conditions and spawn production for the domestication of Macrolepiota detersa | |
| Xin et al. | Alterations in antioxidant enzyme activities and lipid peroxidation induced by diflubenzuron in the carmine spider mite, Tetranychus cinnabarinus (Boisduval)(Acari: Tetranychidae) | |
| CN112471368A (en) | Artificial feed for cowpea pod borer larvae and preparation method thereof | |
| CN103976162A (en) | Artificial feed for soybean pod borers, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170711 Termination date: 20180708 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |