CN105031735A - Small-caliber artificial blood vessel with three-layer composite structure, and preparation method of small-caliber artificial blood vessel - Google Patents
Small-caliber artificial blood vessel with three-layer composite structure, and preparation method of small-caliber artificial blood vessel Download PDFInfo
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Abstract
The invention provides a small-caliber artificial blood vessel with a three-layer composite structure, and a preparation method of the small-caliber artificial blood vessel. The small-caliber artificial blood vessel comprises a weaving inner layer, an electrostatic spinning intermediate layer and a polyurethane outer layer, wherein the electrostatic spinning intermediate layer is arranged on the outer side of the weaving inner layer; the polyurethane outer layer is arranged on the outer side of the electrostatic spinning intermediate layer; the weaving inner layer comprises a first weaving stand pipe and a second weaving bracket stand pipe; the second weaving stand pipe is a weaving stand pipe with axial yarns, and is arranged on the outer side of the first weaving stand pipe. The small-caliber artificial blood vessel adopts a simulation structure; the weaving stand pipes serve as enhancement layers and are relatively high in compliance; the electrostatic spinning intermediate layer adopts a precise three-dimensional reticular structure, so that coated drugs and growth factors are released step by step in the degradation process, thrombosis is prevented, and cell growth is facilitated; the polyurethane outer layer is relatively high in compliance and adopts a microporous structure, so that biomass transmission is facilitated; both PDO and PLLA are degradable materials, so that gradient degradation can be realized, the contact between the polyurethane material and blood can be reduced, the foreign-body reactions to the PDO and PLLA materials can be prevented, and the long-term patency rate can be improved.
Description
Technical field
The present invention relates to a kind of preparation of three-layer composite structure small-caliber artificial blood vessel, it is characterized in that the composite construction artificial blood vessel of design of Simulation, compliance is good, and middle level and internal layer can gradient degradations, reduce the long-term generation of thrombosis, improve long-term patency rate.
Background technology
At present, artificial blood vessel mainly adopts terylene or expanded polytetrafluoroethylsealing material through woven or to be knittingly prepared from, but no matter is that material or forming method all have problems.Polyester material blood compatibility is poor, thrombosis and anastomotic hyperplasia easily occurs and causes blocking; Expanded polytetrafluoroethylsealing hardness is comparatively large, does not mate and easily cause neointimal hyperplasia narrow after implanting with host blood vessel compliance.For small-caliber artificial blood vessel, woven artificial blood vessel's compliance is not good, rigidity is large, fugitive limit, is unfavorable for operation technique and sews up difficulty.Knitting artificial blood vessel surface is comparatively coarse, and flow resistance can be caused to increase, and accelerates the generation of blood coagulation, and knitting artificial blood vessel structure tightness degree is lower simultaneously, and pore-size is comparatively large, easy oozing of blood.Therefore, traditional small-caliber artificial blood vessel clinically problems faced easily forms thrombosis and smooth muscle cell hyperplasia at artificial blood vessel's inwall and causes vascular occlusion to cause graft failure.The elementary tactics of this two hang-up of current solution be external at artificial blood vessel's surface grafting endotheliocyte with endothelialization after promoting Operation.But research shows, the endotheliocyte of planting in expanded polytetrafluoroethylsealing artificial blood vessel surface can discharge some detrimental activity factors in operation process, stimulate smooth muscle cell migration and propagation, finally can cause intimal thickening and obliteration.Therefore, whether external repopulating cell technology is suitable for and still needs to be explored.
Poor in order to solve small-caliber artificial blood vessel antithrombotic, the problems such as smooth muscle cell hyperplasia, desired design goes out a kind of micro-structure surface, can make artificial blood vessel's organizational structure that endotheliocyte depends on.Nonwoven process or molding in integral is adopted to have good effect.Wherein electrostatic spinning technique accurately can obtain two dimension and the three-dimensional micro-nano structure of artificial blood vessel, is widely used at present.The fibre structure that electrostatic spinning obtains is formed by the fiber accumulations of different orientation, and the three dimensional structure of formation is similar to natural extracellular matrix (ECM); Fibre diameter can from tens nanometers to several microns, have that porosity is high, specific surface area large, pore-size distribution is wider, a connective feature such as better between hole simultaneously.And comparatively loose between fiber, when cell adhesion is to fiber surface, the fiber of surrounding can be promoted with extending space, thus improve the cell permeability of material, be conducive to cell intyrusive.Adopt artificial blood vessel prepared by degradation material, promote tissue regeneration while degrading in position, the substitute is autologous tissue.Polyester-amides PEA is dissolved in the mixed solvent of dimethyl formamide by Mo Xiumei etc., prepare electrostatic spinning adsorbable artificial blood vessel, after implanting, growth activity is good, and smooth muscle cell can along fiber growth (number of patent application: CN200810201765.6).The people such as Telemeco have prepared PGA-PLA tissue engineering bracket by method of electrostatic spinning, and by this stenter to implant Mice Body, result shows that this support has good histocompatibility; Xu Weilin etc. have prepared polyethylene terephthalate and polyurethane tubular bracket by method of electrostatic spinning, and inquired into the impact of pipe thickness on mechanical properties such as its compliances, research shows that this tubular bracket is the good scaffold for vascular tissue engineering of a kind of compliance (number of patent application: CN201010565248.4).But Autologous arterial blood pipe, by blood pressure effect, has certain retractility and elasticity, has good radial mechanical performance simultaneously.But the electrostatic spinning artificial blood vessel radial support poor-performing of monolayer, the effect being subject to diastolic pressure and systolic pressure in vivo easily causes artificial blood vessel break and cause massive hemorrhage, causes danger to patient.
The method that global formation prepares micropore artificial blood vessel mainly comprises laser pore method, dipping-percolation, phase separation method etc.The people such as Wang utilize lyophilization multistep processes to obtain porous artificial blood vessel that 3 kinds of internal diameters are 1.5mm, result shows that this loose structure can promote that endotheliocyte adheres to, and endodermis can stride across the end that coincide to interstitial growth.The spraying phase transition method such as Yaguchi obtains small-bore micropore artificial blood vessel, and water transmitance is 26mL/ (mincm
2), meet clinical requirement.Miyamoto etc. by the mixed solution dip-coating of polyurethane and calcium carbonate on glass axle, then glass axle is put into water, remove glass axle core again, finally the polyurethane tube soak with hydrochloric acid obtained is obtained multi-cellular structure artificial blood vessel, its modulus is similar to host blood vessel, in zoopery, there is no neointimal hyperplasia, show good anticoagulant property simultaneously.
Another main cause causing artificial blood vessel's patency rate to decline at present is that elastic properties of materials is poor, causes artificial blood vessel's poor compliance, together cannot beat with host blood vessel, can not adapt to the change of organism environment, and blood is easily at Ink vessel transfusing formation of deposits thrombosis.Polyurethane material good biocompatibility, particularly excellent spring, therefore receive publicity gradually.The elasticity that polyurethane material is good improves the radial diastolic performance of small-caliber artificial blood vessel, makes autologous vein and artificial blood vessel have similar compliance.Grasl etc. have prepared long 140mm by electrostatic spinning, and internal diameter is 2.1mm, and average thickness is the small-bore PU artificial blood vessel of 80 ± 4um, and test finds that endotheliocyte can adhere to and grow on this blood vessel, can express the functional protein of endotheliocyte.The people such as He Wei prepare three-dimensional porous cancellated PU artificial blood vessel by electrostatic spinning, mean porosities is (51.48 ± 4.47) %, axial tensile-strength is (5.85 ± 0.62) Mpa, no cytotoxicity, and is conducive to endothelial cell adhesion and propagation.Jeschke etc. develop internal diameter 1.5mm, the PAUR blood vessel of long 10mm, by with expanded PTFE artificial blood vessel comparing in zoopery, find that this polyurethane blood vessel more easily realizes endothelialization than expanded PTFE blood vessel, induce less neointimal hyperplasia, possess more excellent performance.But polyurethane material also also exists problem.The small-caliber artificial blood vessel prepared of polyurethane material is along with the increase of blood vessel wall thickness, and blood vessel intensity increases, and compliance reduces.Must reduce blood vessel wall thickness to improve compliance, but the decline of wall thickness also reduces the intensity of blood vessel.Contradiction between intensity and compliance limits the application of polyurethane in small-caliber artificial blood vessel.On the other hand, although polyurethane has good cell compatibility, its hydrophilic is poor, and blood compatibility is bad, also can cause thrombosis in vivo with after contacting blood.Method conventional at present carries out hydrophilic modifying to polyurethane material, but modified effect is not desirable especially.
Because existing Weave type artificial blood vessel tube wall mostly is single layer structure, therefore also there is the problem of oozing of blood.Oozing of blood when Ding Xin etc. find to adopt the artificial blood vessel of two-layer different structure can prevent from performing the operation, simultaneously cell tissue can be made again to breed smoothly, and prepare internal layer be warp plain stitch, outer be the Weave type artificial blood vessel (number of patent application: CN200710041266.0) through the two-layer warp-knitted tissue of floss tissue.The medical research of New Jersey Auckland have developed the artificial blood vessel of three-decker, several control of two-dimensional braided layer is sticked together or is stitched together.Japan professor HiromichiSonoda proposes to prepare coaxial double-layer structure small-caliber artificial blood vessel.Obtain highly conforming properties artificial blood vessel by the difference controlling performance on double-layer structure, keep matching with the compliance of autologous vein, increase endothelialization speed, reduce thrombosis and occur, improve long-term patency rate.Result of the test confirms the feasibility of coaxial two-layer small-caliber artificial blood vessel, for the structural design of later artificial blood vessel provides theoretical foundation.China Medical Sciences Academy Fu Wai Hospital professor Hu Shengshou has prepared coaxial three-decker small-caliber artificial blood vessel by coating.The small-caliber artificial blood vessel of coaxial three-decker is prepared on the inside and outside two sides that the PLGA biomaterial with good biocompatibility is coated in polyurethane tube.The people such as You Qingjun are woven into net tubing as middle level with PPDO suture, blended chondroitin sulfate-the collagenic coating of internal layer, outer bag, by matrix fiber layer, is reinforced with suture, is compounded to form small-caliber artificial blood vessel (number of patent application: CN200810244720.7).Artificial blood vessel's biocompatibility of a series of characterization result display preparation and good mechanical properties, reach the requirement of Clinical practice substantially.Compared with traditional monolayer artificial blood vessel, three-decker has following characteristics: can control hole gap rate, loosing-resistant, has good self-supporting structure, can prevent from expanding and subsiding, and has longitudinal compatibility, is easy to transplant.
Other weaving pipeline forming method relatively, Weaving type pipeline fabric inner surface is comparatively bright and clean, compliance is good, available thicker yarn is prepared simultaneously, improve the radial support performance of pipeline fabric on the one hand, reduce the specific surface area of material on the other hand, prevent platelet and fibrinous over-deposit.Braiding structure is introduced in the preparation of artificial blood vessel by the present invention, for improving artificial blood vessel's radial strength, solves the contradiction between small-caliber artificial blood vessel intensity and compliance.Design braiding structure simultaneously, utilize the feature of knitting forming, be prepared into two-layer composite braided pipeline, internal layer is rule braiding pipeline, and the axial yarn of outer introducing, by introducing axle yarn increase space between layers, Promote cell's growth.
Summary of the invention
The object of the invention is to solve the poor and polyurethane artificial blood vessel Problems existing of present stage small-caliber artificial blood vessel compliance, a kind of artificial blood vessel of three-layer composite structure is provided, promote the quick growth of cell, dissolve between intensity and compliance and while contradiction, avoid polyurethane and contacting blood.
In order to achieve the above object, the invention provides a kind of three-layer composite structure small-caliber artificial blood vessel, it is characterized in that, comprise braiding internal layer, the outside of braiding internal layer is provided with electrostatic spinning middle level, and the outside in electrostatic spinning middle level is provided with polyurethane outer, wherein, described braiding internal layer comprises the first braided support pipe and is located at the second braided support pipe in outside of the first braided support pipe, and the second described braided support pipe is tape spool yarn braided support pipe.
Preferably, described polyurethane outer distributes porose.
Preferably, the first described braided support pipe to be braid angle be PDO (PPDO) support tube of 60 ° ~ 70 °, the second described braided support pipe is three-dimensional braiding, is the PDO support tube of 30 ° ~ 45 ° with axial yarn, braid angle.
Preferably, the internal diameter of described braiding internal layer is 4-6mm, wall thickness is 0.6-1.0mm, and the wall thickness in electrostatic spinning middle level is 0.2-0.5mm, and the wall thickness of polyurethane outer is 0.3-0.5mm.
Present invention also offers a kind of preparation method of three-layer composite structure small-caliber artificial blood vessel, it is characterized in that, concrete steps comprise:
Step 1: adopt PDO monofilament as knitting yarn, spindle type braider is prepared the first braided support pipe, and carries out thermal finalization process;
Step 2: introduce axle yarn on spindle type braider, at the outer surface of the first braided support pipe, weaves the second braided support pipe, forms braiding internal layer, and carries out thermal finalization process;
Step 3: preparation PLLA spinning solution, adopts electrostatic spinning technique to form one deck electrostatic spinning middle level at the skin of braiding internal layer;
Step 4: preparation polyurethane solutions, forms polyurethane outer in the outside in electrostatic spinning middle level, obtains three-layer composite structure small-caliber artificial blood vessel.
Preferably, the spindle type braider in described step 1 is two-dimentional spindle type braider.
Preferably, the diameter of the PDO monofilament in described step 1 is 0.20-0.30mm.
Preferably, containing PEG in the PLLA spinning solution in described step 3.Described PEG is for improving the hydrophobicity of PLLA.
More preferably, the weight ratio of described PLLA and PEG is 7: 3.
Preferably, the solvent of the PLLA spinning solution in described step 3 is volatile solvent, and PLLA solution concentration is 2-5%.
More preferably, described volatile solvent is CH
2cl
2.
Preferably, the electrospinning conditions in described step 3 is: applied voltage is 7-15kV, spinning flow velocity is 1-5mL/h, receiving range is 15-20cm.
Preferably, containing at least one in antithrombotic reagent, anti-neointimal hyperplasia medicine, the medicine of anti-inflammatory response or the somatomedin of Promote cell's growth in the PLLA spinning solution in described step 3.
Preferably, containing at least one in heparin, hirudin, paclitaxel and rapamycin in the PLLA spinning solution in described step 3.
Preferably, containing salt in polyurethane solutions in described step 4, the weight ratio of salt and polyurethane is 4-8: 1, the concentration of polyurethane solutions is 20%-30%, the step that the described outside in electrostatic spinning middle level forms polyurethane outer comprises: by polyurethane solutions brushing outside electrostatic spinning middle level, put into 50 DEG C of-60 DEG C of water and separate out salt grain, dry formation polyurethane outer.
Preferably, the outside in electrostatic spinning middle level in described step 4 forms polyurethane outer and adopts dipping-percolation.
Multilamellar artificial blood vessel internal diameter prepared by the present invention and each layer wall thickness carry out rational design according to actual needs, to coordinate specific function.
Prepared by the present invention is multi-layer compound structure small-caliber artificial blood vessel.The preparation proposed by Japanese professor HiromichiSonoda is theoretical, in conjunction with the advantage of different textile structural, prepares the emulated artificial blood vessel more mating autologous vein structure.Braided support, as enhancement layer, can be resisted autologous vein diastole and shrink the vascular pressure produced, having good compliance simultaneously; Electrostatic spinning layer has accurate tridimensional network, and in the process of degraded, coated medicine and somatomedin progressively discharge, and prevent thrombosis and Promote cell's growth; Layer of polyurethane has good compliance and microcellular structure, contributes to the transmission of somatomedin, nutrient substance and metabolic waste.The PDO that braided support is used and electrostatic spinning PLLA used is degradation material, can realize gradient degradation, reduces polyurethane material and contacting blood, and the foreign body reaction simultaneously preventing from material to be in producing in body also improves long-term patency rate.
After multilayer materials artificial blood vessel involved in the present invention replaces host blood vessel by operation, smooth muscle cell and endotheliocyte will from artificial blood vessel two ends to multiple structure between grow, and pass through the micropore on electrostatic spinning layer and layer of polyurethane surface to somatomedin, nutrient substance and metabolic waste transmit, and the platelet etc. in blood can be adsorbed on braid and the electrostatic spinning layer of internal layer, by release somatomedin, contribute to the further reparation of inner membrance, effectively reduce the probability of polyurethane and contacting blood simultaneously, reduce the problem that polyurethane material blood compatibility difference causes.Through the time PPDO braided support degraded of about a year completely, through the time PLLA electrostatic spinning layer degraded of about 2 years completely, reduce long-term foreign body reaction, improve long-term patency rate.
Compared with prior art, the present invention has following beneficial effect:
(1) three-layer composite structure small-caliber artificial blood vessel involved in the present invention, internal layer and middle level can realize gradient degradation, outer polyurethane layer has good elasticity and compliance, has design of Simulation, improves the advantage such as artificial blood vessel's compliance and long-term patency rate;
(2) technical method that three-layer composite structure small-caliber artificial blood vessel involved in the present invention adopts comprises weaving knitting skill, electrostatic spinning technique and integral shaping method, in conjunction with the advantage of different weaving forming method, can reach good result of use;
(3) three-layer composite structure small-caliber artificial blood vessel surface involved in the present invention has microcellular structure, and aperture is less, without the need to solidifying process in advance, reduces the operation waiting time, is conducive to improving success rate of operation;
(4) three-layer composite structure artificial blood vessel involved in the present invention can carry out the control of the physical dimension such as internal diameter, tube wall, can directional preparation to different pathological environment.
Accompanying drawing explanation
Fig. 1 a is three-layer composite structure small-caliber artificial blood vessel structural representation;
Fig. 1 b is braiding endothecium structure schematic diagram;
Fig. 1 c is electrostatic spinning media layer damage schematic diagram;
Fig. 1 d is polyurethane outer structural representation.
Fig. 2 is the transverse sectional view of three-layer composite structure small-caliber artificial blood vessel.
Fig. 3 a is two-layer braiding compound rest structural representation;
Fig. 3 b is the first braided support tubular construction schematic diagram;
Fig. 3 c is the second braided support tubular construction schematic diagram.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
As shown in Figure 1a, three-layer composite structure small-caliber artificial blood vessel of the present invention, is made up of braiding internal layer 1, electrostatic spinning middle level 2 and polyurethane outer 3, by braiding internal layer 1, electrostatic spinning middle level 2 and polyurethane outer 3 structure as shown in figs. lb-1d.As shown in Figure 2, the outside of braiding internal layer 1 is provided with electrostatic spinning middle level 2, the outside in electrostatic spinning middle level 2 is provided with polyurethane outer 3, wherein, as shown in Figure 3 a, described braiding internal layer 1 comprises the first braided support pipe 11 as shown in Figure 3 b and is located at the second braided support pipe 12 as shown in Figure 3 c in outside of the first braided support pipe 11, and the second described braided support pipe 12 is tape spool yarn braided support pipe.Described polyurethane outer 3 distributes porose.
The preparation method of above-mentioned three-layer composite structure small-caliber artificial blood vessel is:
Step 1: on 32 ingot two dimension spindle type braiders, employing diameter is that the PDO monofilament of 0.30mm weaves as knitting yarn the first braided support pipe 11 that internal diameter is 4mm, through 100 DEG C in forced air oven, 10min thermal finalization process, the braid angle of the first braided support pipe 11 is 65 °, and external diameter is 4.5mm;
Step 2: on 16 ingot two dimension spindle type braiders, diameter is adopted to be that the PDO monofilament of 0.30mm is as knitting yarn and axial yarn, at the outer surface of the first braided support pipe 11, weave the second braided support pipe 12, form braiding internal layer 1, through 100 DEG C in forced air oven, 10min thermal finalization process, the braid angle of the second braided support pipe 12 is 45 °, and external diameter is 5.0mm;
Step 3: preparation PLLA spinning solution: by PLLA (molecular weight is 1,500,000), be dissolved in CH
2cl
2add PEG (molecular weight is 800,000) and heparin, be uniformly mixed, obtain PLLA spinning solution, wherein, PLLA mass fraction is 2%, the weight ratio of PLLA and PEG is 7: 3, the weight ratio of PLLA and heparin is 100: 1, and adopt electrostatic spinning technique to form one deck electrostatic spinning middle level 2 at the skin of braiding internal layer 1, electrospinning conditions is: applied voltage is 10kV, spinning flow velocity is 5mL/h, receiving range is 20cm;
Step 4: preparation polyurethane solutions: by polyurethane (molecular weight is 4.2 ten thousand), add in dimethylacetamide solvent, add sodium chloride powder, stir and obtain suspension, the weight ratio of sodium chloride and polyurethane is 6: 1, the concentration of polyurethane solutions is 25%, polyurethane solutions is evenly brushed for several times at electrostatic spinning middle level 2 outer surface repeatedly, put into 50 DEG C of water and separate out salt grain, form polyurethane outer 3 in the outside in electrostatic spinning middle level 2 after drying, obtain three-layer composite structure small-caliber artificial blood vessel.
The internal diameter of the described braiding internal layer 1 of the three-layer composite structure small-caliber artificial blood vessel of gained is 4mm, wall thickness is 1.0mm, wherein the first braided support pipe 11 for braid angle be the PDO support tube of 65 °, internal diameter is 4mm, external diameter is 4.5mm, the second described braided support pipe 12 is three-dimensional braiding, is the PDO support tube of 45 ° with axial yarn, braid angle, and external diameter is 5.0mm.The wall thickness in electrostatic spinning middle level 2 is 0.2mm, and external diameter is 5.2mm, and the wall thickness of polyurethane outer 3 is 0.3mm, and external diameter is 5.5mm.
The fibre diameter in the electrostatic spinning middle level 2 of the three-layer composite structure small-caliber artificial blood vessel of gained is 100-200nm, the outer wall aperture of polyurethane outer 3 is (140 ± 41 μm), inwall aperture is (100 ± 3 μm), porosity is 79.8%, and radial Cdgn dyanamic compliance is 8.82%.
The present invention is at the implant into body initial stage, and due to the existence of braid and electrostatic spinning layer, layer of polyurethane does not contact blood substantially, prevents the blood compatibility difference due to polyurethane from causing blood coagulation to produce; Implant after 1-2, along with the degraded gradually of braid and electrostatic spinning layer, cell progressively in the growth of layer of polyurethane inner surface and basic endothelialization completes, and the good compliance of layer of polyurethane ensure that the long-term patency rate of artificial blood vessel.
Claims (10)
1. a three-layer composite structure small-caliber artificial blood vessel, it is characterized in that, comprise braiding internal layer (1), the outside of braiding internal layer (1) is provided with electrostatic spinning middle level (2), the outside in electrostatic spinning middle level (2) is provided with polyurethane outer (3), wherein, described braiding internal layer (1) comprises the first braided support pipe (11) and is located at the second braided support pipe (12) in outside of the first braided support pipe (11), and the second described braided support pipe (12) is tape spool yarn braided support pipe.
2. three-layer composite structure small-caliber artificial blood vessel as claimed in claim 1, is characterized in that, the upper distribution of described polyurethane outer (3) is porose.
3. three-layer composite structure small-caliber artificial blood vessel as claimed in claim 1, it is characterized in that, the first described braided support pipe (11) for braid angle be the PDO support tube of 60 ° ~ 70 °, the second described braided support pipe (12) is three-dimensional braiding, is the PDO support tube of 30 ° ~ 45 ° with axial yarn, braid angle.
4. three-layer composite structure small-caliber artificial blood vessel as claimed in claim 1, it is characterized in that, the internal diameter of described braiding internal layer (1) is 4-6mm, wall thickness is 0.6-1.0mm, the wall thickness in electrostatic spinning middle level (2) is 0.2-0.5mm, and the wall thickness of polyurethane outer (3) is 0.3-0.5mm.
5. the preparation method of the three-layer composite structure small-caliber artificial blood vessel according to any one of claim 1-4, it is characterized in that, concrete steps comprise:
Step 1: adopt PDO monofilament as knitting yarn, spindle type braider is prepared the first braided support pipe (11), and carries out thermal finalization process;
Step 2: introduce axle yarn on spindle type braider, at the outer surface of the first braided support pipe (11), weaves the second braided support pipe (12), forms braiding internal layer (1), and carries out thermal finalization process;
Step 3: preparation PLLA spinning solution, adopts electrostatic spinning technique to form one deck electrostatic spinning middle level (2) at the skin of braiding internal layer (1);
Step 4: preparation polyurethane solutions, forms polyurethane outer (3) in the outside of electrostatic spinning middle level (2), obtains three-layer composite structure small-caliber artificial blood vessel.
6. the preparation method of three-layer composite structure small-caliber artificial blood vessel as claimed in claim 5, it is characterized in that, the spindle type braider in described step 1 is two-dimentional spindle type braider.
7. the preparation method of three-layer composite structure small-caliber artificial blood vessel as claimed in claim 5, it is characterized in that, the diameter of the PDO monofilament in described step 1 is 0.20-0.30mm.
8. the preparation method of three-layer composite structure small-caliber artificial blood vessel as claimed in claim 5, is characterized in that, containing PEG in the PLLA spinning solution in described step 3.
9. the preparation method of three-layer composite structure small-caliber artificial blood vessel as claimed in claim 5, it is characterized in that, containing at least one in antithrombotic reagent, anti-neointimal hyperplasia medicine, the medicine of anti-inflammatory response or the somatomedin of Promote cell's growth in the PLLA spinning solution in described step 3.
10. the preparation method of three-layer composite structure small-caliber artificial blood vessel as claimed in claim 5, it is characterized in that, containing salt in polyurethane solutions in described step 4, the weight ratio of salt and polyurethane is 4-8: 1, the concentration of polyurethane solutions is 20%-30%, the step that the described outside in electrostatic spinning middle level (2) forms polyurethane outer (3) comprises: by polyurethane solutions brushing in electrostatic spinning middle level (2) outside, put into 50 DEG C of-60 DEG C of water and separate out salt grain, dry formation polyurethane outer (3).
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Effective date of registration: 20240422 Address after: Room 805, Zone E, 8th Floor, Building 5, No. 2278 Zhaozhong Road, Zhonggu Town, Qingpu District, Shanghai, June 2017 Patentee after: Shanghai Youmaichuang Technology Co.,Ltd. Country or region after: China Address before: 201620 No. 2999 North Renmin Road, Shanghai, Songjiang District Patentee before: DONGHUA University Country or region before: China |