CN104956909B - A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum - Google Patents
A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum Download PDFInfo
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- CN104956909B CN104956909B CN201510453975.4A CN201510453975A CN104956909B CN 104956909 B CN104956909 B CN 104956909B CN 201510453975 A CN201510453975 A CN 201510453975A CN 104956909 B CN104956909 B CN 104956909B
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- Prior art keywords
- scale
- cuttage
- cutting
- detoxification
- lilium longiflorum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum, realizes that the technology comprises the steps：Detoxification Lilium longiflorum bulb Storage in cold bank, scale sterilization, scale cutting, cutting scale cuttage, cuttage Bin covering black thin film after inserting.Cutting scale can breed 2 seed balls inorganic without native Light media perlite cuttage asexual propagation, and full scale lining coefficient can be up to 4 ~ 6.There is advantages of the present invention asexual propagation seed ball the harms of microbe such as detoxification Lilium longiflorum detoxification quality, reduction cuttage process virus, the full scale lining coefficient of raising, reduction hormone can be kept to remain in scale or seed ball, save scale, reduces cost.
Description
Technical field
The present invention relates to a kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum, platymiscium cuttage vegetative propagation technique
Field.
Background technology
One of Lilium longiflorum, is edible lily place one of microspecies, and Jiangxi Ways of Special Agricultural Products, can eat raw, can process
Cheng Fen, can add and make noodles etc., can also be processed into all multipurposes such as beverage.Lilium longiflorum bulb price is higher.Produce at present
In, the breeding of detoxification Lilium longiflorum has 2 kinds of common methods, full scale or full scale to dip phytohormone cuttage and Lilium longiflorum tissue culture
Etc. mode.With ecommerce and food diversified development, Lilium longiflorum bulb and the converted productss market demand potential are big.Especially
As food-safety problem is increasingly paid attention to, food safety raw material production is the first link.The full scale cuttage of traditional Bulbus Lilii is present
Some problems, CN104663190A disclose a kind of full scale lining method of Herba Crotalariae sessiliflorae, but the method to there is scale utilization rate low
Problem.CN101711493A discloses a kind of vegetative propagation technique for directly stripping scale lining lily ball, but this side
Method has that breeding coefficient is low.CN102577804A discloses a kind of method for improving rate of scale cuttage propagation of lily.Should
Patent mainly takes hot water treatment, various chemical medicaments process, using measures such as organic substrates for viewing and admiring oriental hybrid lily kind
Its bird watching kind cutting propagation rate is improved, and full scale cuttage breeding coefficient is low, typically only 2；Fancy breed is unlike de-
Malicious Lilium longiflorum edible lily kind considers food safety and adopts physical disinfection more and inorganic arrange without native Light media perlite etc.
Apply.CN102577804 patents are also not directed to detoxification Lilium longiflorum edible variety, K cryogenic treatment, scale physical disinfection, inorganic without soil
The technology such as each cutting scale cuttage breeding seed ball after Light media perlite, scale cutting.
Existing plant cuttage will typically adopt hormone, but as the Lilium longiflorum that food is used, just should not be using normal
The HORMONE TREATMENT of rule cuttage.Existing Bulbus Lilii class plant scale cuttage typically adopts full scale cuttage, its full scale lining coefficient
General only 2, cause scale to waste, cost increases.
The content of the invention
The purpose of the present invention is, has for current Lilium longiflorum cutting propagation that hormone residues, scale utilization rate be low, breeding
Coefficient is low, high cost the problems such as.The present invention provides a kind of detoxification Lilium longiflorum safe and efficient scale cuttage technology.
The present invention is achieved through the following technical solutions：The inventive method includes scale sterilization, scale cutting and cutting squama
Piece cuttage, it is also inorganic asexual numerous without native Light media perlite cuttage including many cuttings of K cryogenic treatment and scale before scale cuttage
Grow.
The scale is disinfected, and is soaked using chitosan solution；
The many cuttings of the scale are processed, and outer layer and middle level scale are cut into independent 3 sections, i.e. 1/3 scale along vertical long axis；It is interior
Layer scale is cut into independent 2 sections, i.e. 1/2 scale along vertical long axis.
A kind of safe and efficient scale cuttage technical step of detoxification Lilium longiflorum of the present invention is as follows：
（1）Cold storage of seedball
August harvests the 3 years raw cultivation commodity bulbs in detoxification Lilium longiflorum tissue cultured seedling land for growing field crops, select it is disease-free, without wound, quality
Up to 35 g ~ 50 g detoxification Lilium longiflorum bulbs, the bulb exposure with a small amount of soil 2 days is put into 5 DEG C of Storage in cold bank afterwards.Cuttage
2 DEG C of low-temperature cold stores of front need are processed 2 days.
（2）Scale is sterilized
Without the detoxification Lilium longiflorum bulb such as disease-free that rots after taking cold preservation, scale is gently peeled from outside to inside, 100 are put into
Ppm shitosans soak 3 hours.And it is gently mixed solution.
（3）Scale cutting
With sterilizing, outer layer and middle level scale are cut into independent 3 sections, i.e. 1/3 scale along vertical long axis by knife；Internal layer scale edge
Vertical long axis are cut into independent 2 sections, i.e. 1/2 scale.Under room temperature, after 3 hours, cutting scale is killed virus with uviol lamp in cool place.
（4）Cutting scale cuttage
It is before cutting scale cuttage, fresh inorganic without the exposure in 3 days in advance of native Light media perlite, by exposure inorganic without native light base
Matter perlite loads treats cuttage bubble chamber, and appropriate compacting pearlite interstitial substance, and with 50 ppm shitosans spraying stromal surface.
Cutting scale without the need for Plant hormone treatment, by the cuttage down of cutting scale lower end biology in inorganic without native Light media Margarita batholith
Matter, 3 cm of cuttage depth, 5 cm × 5 cm of cuttage seeding row spacing.Cutting case surrounding leaves pore.
（5）Black thin film is covered after inserting
After cutting scale cuttage, inorganic without native Light media pearlite interstitial substance surface spray moisturizing, black thin film is sprayed and is hung
There is water droplet, cover cutting case immediately.Observation substrate and black film humidity, are sprayed as one sees fit, and after cuttage, cutting case is put in vinyl house.
（6）Seed ball culture
After waiting cutting scale lower end biology to grow 2 white seed balls, i.e., full scale lining coefficient is up to 4 ~ 6.Solution except black
Color thin film is covered, grown cultures management under the conditions of normal management in booth.
The invention has the beneficial effects as follows, the inventive method is inorganic asexual without native Light media perlite cuttage using cutting scale
Breeding, can breed 2 seed balls, i.e., up to 2, full scale lining coefficient is up to 4 ~ 6 for each cut-out scale lining coefficient；Save squama
Piece, reduces cost.
The present invention adopts shitosan soaking disinfection to scale, can prevent pollution of the disinfectant solution to Lilium longiflorum；Cuttage process
Hormone is not adopted, prevents hormone from remaining in scale or seed ball；Ensure the edible safety standard of Lilium longiflorum.
Specific embodiment
A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum of the present invention, its specific embodiment include following step
Suddenly：
（1）Cold storage of seedball
After August detoxification Lilium longiflorum land for growing field crops aerial partss are withered, the raw cultivation in 3 years of detoxification Lilium longiflorum tissue cultured seedling land for growing field crops is harvested
Commodity bulb, select it is disease-free, without wound, scale length and hypertrophy, quality up to the fresh bulb of detoxification Lilium longiflorum such as 35 g ~ 50 g,
By the fresh bulb exposure 2 days of band a small amount of soil, 5 DEG C or so Storage in cold bank are put into afterwards.Certain space is kept between fresh bulb.
2 DEG C of freezer K cryogenic treatment 2 days before cuttage.
（2）Scale is sterilized
Select, without the fresh bulb of K cryogenic treatment detoxification Lilium longiflorum such as disease-free that rots, gently to peel scale from outside to inside, gently
Put down gently and soak 3 hours into 100 ppm shitosans.Period is simultaneously gently mixed solution.
（3）Scale cutting
Outer layer and middle level scale are averagely cut into independent 3 sections i.e. 1/3 scale along vertical long axis by high-temperature sterilization knife（Cut upper end
Section, middle cutting, lower end cutting）, 1/3 cutting scale mean fresh, 1.11 g；Internal layer scale is cut into independent 2 sections along vertical long axis
That is 1/2 scale（Upper end cutting and lower end cutting）, 1/2 cutting scale mean fresh 1.66g.Under room temperature, cool place was cut after 1 hour
Section scale uviol lamp is killed virus 10 seconds.
（4）Cutting scale cuttage
It is fresh inorganic without the exposure in 3 days in advance of native Light media perlite, treat exposure inorganic without the loading of native Light media perlite
Foam cutting case, appropriate compacting pearlite interstitial substance, and with 50 ppm shitosans spray stromal surface.Cutting scale is without the need for plant
HORMONE TREATMENT, by cutting scale lower end biology down gently cuttage in inorganic without in native Light media pearlite interstitial substance, cuttage depth
3 cm are spent, cutting scale does not expose, 5 cm × 5 cm of seeding row spacing.The design of cutting case surrounding leaves pore.
（5）Black thin film is covered after inserting
After cuttage, inorganic without native Light media pearlite interstitial substance surface spray moisturizing, spraying black thin film to be covered simultaneously hangs with
Water droplet, black film cover cutting case immediately.Observation soil humidity and black film humidity, are sprayed as one sees fit, and after cuttage, cutting case is put into plastics
In canopy.
（6）Seed ball culture
After waiting cutting scale lower end biology to grow 2 white seed balls, i.e., full scale lining coefficient is up to 4 ~ 6.Solution except black
Film is covered, and Small plastic shed is taken outside cutting case, grown cultures and management detoxification Lilium longiflorum clove seed ball under the conditions of normal management.Skewer
After being inserted to culture April, each scale lining bulb seed ball growing state that processes is shown in Table 1.
Seed ball after detoxification Lilium longiflorum cutting scale safe and efficient cuttage April（Bulb seed ball）Growth indexes such as following table institute
Show：
。
Claims (1)
1. the sterilization of a kind of safe and efficient scale cuttage method of detoxification Lilium longiflorum, including scale, scale cutting and scale cuttage, its
Be characterised by, methods described include the K cryogenic treatment before scale cuttage and cutting scale it is inorganic without the cuttage of native Light media perlite without
Sexual reproduction；
August is harvested the 3 years raw cultivation commodity kinds in detoxification Lilium longiflorum tissue cultured seedling land for growing field crops by the K cryogenic treatment before the scale cuttage
Ball, selection is disease-free, 35g～50g detoxification Lilium longiflorum bulbs are reached without wound, quality, the bulb exposure with a small amount of soil 2 days,
5 DEG C of Storage in cold bank are put into afterwards, need 2 DEG C of low-temperature cold stores to process before cuttage 2 days；
The scale sterilization, takes the rotten disease-free detoxification Lilium longiflorum bulb of nothing after cold preservation, gently peels scale from outside to inside, put
Enter 100ppm shitosans to soak 3 hours；
Outer layer and middle level scale are cut into independent 3 sections, i.e. 1/3 cutting scale along vertical long axis by the scale cutting；Internal layer scale
Independent 2 sections, i.e. 1/2 cutting scale are cut into along vertical long axis；
It is described inorganic without native Light media perlite cuttage asexual propagation, it is before cutting scale cuttage, fresh inorganic without native Light media
Perlite shifts to an earlier date exposure in 3 days, treats cuttage bubble chamber, and appropriate compacting perlite without the loading of native Light media perlite by exposure inorganic
Substrate, and with 50ppm shitosans spray stromal surface；Cutting scale without the need for Plant hormone treatment, by under cutting scale biology
End cuttage down is in inorganic without native Light media pearlite interstitial substance, cuttage depth 3cm, cuttage seeding row spacing 5cm × 5cm；Cutting case four
Zhou Liuyou pores.
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| CN201510453975.4A CN104956909B (en) | 2015-07-30 | 2015-07-30 | A kind of safe and efficient scale cuttage technology of detoxification Lilium longiflorum |
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| CN104956909A CN104956909A (en) | 2015-10-07 |
| CN104956909B true CN104956909B (en) | 2017-03-29 |
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| CN105325272A (en) * | 2015-10-25 | 2016-02-17 | 湖北京穗农林科技发展有限公司 | Lagerstroemia indica cutting breeding method |
| CN106171878A (en) * | 2016-06-30 | 2016-12-07 | 贵州省普安县普白珍稀资源开发有限公司 | A kind of method of Lilies breeding |
| CN106688657A (en) * | 2017-01-05 | 2017-05-24 | 江西省科学院生物资源研究所 | Method of breeding seedball with longya lily scalesunder LED illuminant |
| CN107466628A (en) * | 2017-08-02 | 2017-12-15 | 广西壮族自治区林业科学研究院 | A kind of method of the efficient cutting propagation of giant lily scale |
| CN110959493A (en) * | 2019-11-13 | 2020-04-07 | 湖南工业大学 | Method for preventing lily from degeneration |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH1033078A (en) * | 1996-05-22 | 1998-02-10 | Ishikawajima Harima Heavy Ind Co Ltd | Production of bulb of liliaceae plant |
| CN103141381A (en) * | 2013-02-24 | 2013-06-12 | 周世永 | Tissue culture method for oriental lily |
| CN103563611A (en) * | 2013-07-24 | 2014-02-12 | 王运海 | Planting method for lilies |
| CN104663190B (en) * | 2013-12-03 | 2017-05-10 | 铜仁学院 | Wild lily bulb scale propagation method |
| CN103907522B (en) * | 2014-04-04 | 2016-08-03 | 天津农学院 | A kind of edible lily organic soilless cultivation matrix formulations and cultural method |
| CN104115637A (en) * | 2014-06-25 | 2014-10-29 | 湖南省宝庆农产品进出口有限公司 | Dried lily bulb cultivation method |
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