CN104920360A - Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen - Google Patents
Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen Download PDFInfo
- Publication number
- CN104920360A CN104920360A CN201510341914.9A CN201510341914A CN104920360A CN 104920360 A CN104920360 A CN 104920360A CN 201510341914 A CN201510341914 A CN 201510341914A CN 104920360 A CN104920360 A CN 104920360A
- Authority
- CN
- China
- Prior art keywords
- bufexamac
- bactericide
- plant disease
- preparation
- pathogenic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- MXJWRABVEGLYDG-UHFFFAOYSA-N bufexamac Chemical compound CCCCOC1=CC=C(CC(=O)NO)C=C1 MXJWRABVEGLYDG-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 229960000962 bufexamac Drugs 0.000 title claims abstract description 58
- 201000010099 disease Diseases 0.000 title claims abstract description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 37
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 29
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims description 40
- 241000894006 Bacteria Species 0.000 claims description 33
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 21
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 21
- 235000021014 blueberries Nutrition 0.000 claims description 21
- 230000001717 pathogenic effect Effects 0.000 claims description 20
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 19
- 240000003768 Solanum lycopersicum Species 0.000 claims description 19
- 235000011430 Malus pumila Nutrition 0.000 claims description 18
- 235000015103 Malus silvestris Nutrition 0.000 claims description 18
- 240000000851 Vaccinium corymbosum Species 0.000 claims description 18
- 241000233866 Fungi Species 0.000 claims description 15
- 244000070406 Malus silvestris Species 0.000 claims description 14
- 244000052769 pathogen Species 0.000 claims description 14
- 230000000813 microbial effect Effects 0.000 claims description 13
- 240000008067 Cucumis sativus Species 0.000 claims description 11
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 11
- 241000123650 Botrytis cinerea Species 0.000 claims description 9
- 244000061176 Nicotiana tabacum Species 0.000 claims description 8
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 8
- 235000021307 Triticum Nutrition 0.000 claims description 8
- 241000223195 Fusarium graminearum Species 0.000 claims description 6
- 208000025865 Ulcer Diseases 0.000 claims description 6
- 231100000397 ulcer Toxicity 0.000 claims description 6
- 244000144730 Amygdalus persica Species 0.000 claims description 5
- 241000193738 Bacillus anthracis Species 0.000 claims description 5
- 235000006040 Prunus persica var persica Nutrition 0.000 claims description 5
- 241000555706 Botryosphaeria dothidea Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 241001330975 Magnaporthe oryzae Species 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 241000220324 Pyrus Species 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000021017 pears Nutrition 0.000 claims description 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 17
- 238000011161 development Methods 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000003912 environmental pollution Methods 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 244000000004 fungal plant pathogen Species 0.000 abstract description 3
- 231100000053 low toxicity Toxicity 0.000 abstract description 3
- 241000282414 Homo sapiens Species 0.000 abstract description 2
- 235000012055 fruits and vegetables Nutrition 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 206010059866 Drug resistance Diseases 0.000 abstract 2
- 244000144972 livestock Species 0.000 abstract 1
- -1 small molecule compound Chemical class 0.000 abstract 1
- 239000000575 pesticide Substances 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 16
- 230000003385 bacteriostatic effect Effects 0.000 description 12
- 230000006378 damage Effects 0.000 description 11
- 230000012010 growth Effects 0.000 description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 8
- 241000209140 Triticum Species 0.000 description 7
- 230000006872 improvement Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 244000081841 Malus domestica Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 238000012271 agricultural production Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 201000004624 Dermatitis Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 244000077233 Vaccinium uliginosum Species 0.000 description 3
- 235000010724 Wisteria floribunda Nutrition 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000855 fungicidal effect Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000000041 non-steroidal anti-inflammatory agent Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 241000893212 Pestalotia Species 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000001408 fungistatic effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229940021182 non-steroidal anti-inflammatory drug Drugs 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000006072 paste Substances 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 206010039073 rheumatoid arthritis Diseases 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- NEAQRZUHTPSBBM-UHFFFAOYSA-N 2-hydroxy-3,3-dimethyl-7-nitro-4h-isoquinolin-1-one Chemical compound C1=C([N+]([O-])=O)C=C2C(=O)N(O)C(C)(C)CC2=C1 NEAQRZUHTPSBBM-UHFFFAOYSA-N 0.000 description 1
- 241000208140 Acer Species 0.000 description 1
- 235000006667 Aleurites moluccana Nutrition 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- MWLYPKOCGSRFMH-UHFFFAOYSA-N C(C)(=O)NC1=CC=CC=C1.[O] Chemical compound C(C)(=O)NC1=CC=CC=C1.[O] MWLYPKOCGSRFMH-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 244000062995 Cassia occidentalis Species 0.000 description 1
- 235000001948 Cassia occidentalis Nutrition 0.000 description 1
- 240000004957 Castanea mollissima Species 0.000 description 1
- 235000018244 Castanea mollissima Nutrition 0.000 description 1
- QCDFBFJGMNKBDO-UHFFFAOYSA-N Clioquinol Chemical compound C1=CN=C2C(O)=C(I)C=C(Cl)C2=C1 QCDFBFJGMNKBDO-UHFFFAOYSA-N 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 229940122964 Deacetylase inhibitor Drugs 0.000 description 1
- 244000004281 Eucalyptus maculata Species 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000218652 Larix Species 0.000 description 1
- 235000005590 Larix decidua Nutrition 0.000 description 1
- 235000005087 Malus prunifolia Nutrition 0.000 description 1
- 244000134242 Malus prunifolia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 244000132436 Myrica rubra Species 0.000 description 1
- 201000009053 Neurodermatitis Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 244000294611 Punica granatum Species 0.000 description 1
- 235000014360 Punica granatum Nutrition 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 235000012511 Vaccinium Nutrition 0.000 description 1
- 241000736767 Vaccinium Species 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 230000001741 anti-phlogistic effect Effects 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 244000046738 asparagus lettuce Species 0.000 description 1
- 235000006705 asparagus lettuce Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- UREBDLICKHMUKA-DVTGEIKXSA-N betamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-DVTGEIKXSA-N 0.000 description 1
- 229960002537 betamethasone Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- FEBLZLNTKCEFIT-VSXGLTOVSA-N fluocinolone acetonide Chemical compound C1([C@@H](F)C2)=CC(=O)C=C[C@]1(C)[C@]1(F)[C@@H]2[C@@H]2C[C@H]3OC(C)(C)O[C@@]3(C(=O)CO)[C@@]2(C)C[C@@H]1O FEBLZLNTKCEFIT-VSXGLTOVSA-N 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000003779 hair growth Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000003637 steroidlike Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the application of bufexamac in preparation of a bactericide used for controlling a plant disease caused by phytopathogen. It is proved that bufexamac has the good inhibitory activity on plant pathogenic fungi through indoor toxicity measurement. The bufexamac used as the bactericide has the advantages of high efficiency and low toxicity, and meets the requirement for plant disease chemical control. At present, a large number of bactericides are used, phytopathogen drug resistance is enhanced, and traditional bactericides are high in environmental pollution and residue and directly threaten the food safety of human beings. However, the bufexamac is a small molecule compound which is degradable, free of pollution, environmentally friendly, poor in drug resistance, safe for non-target organisms, people and livestock, capable of ensuring high quality of agricultural products, fruits and vegetables and meeting the requirement for sustainable development, and wide in search and market application prospect.
Description
Technical field
The present invention relates to Fungicidal active compound, particularly bufexamac for the preparation of control by the purposes in the bactericide of the microbial plant disease of pathogenic.
Background technology
Agricultural is the basis of human society and economic development, and sustainable agriculture is the trend of world agriculture development.Since 20th century, along with the raising of scientific and technological progress and social productive forces, the mankind create unprecedented wealth, meanwhile, the problems such as population increase, shortage of resources, environmental pollution, ecological disruption and frequent natural calamity become increasingly conspicuous, seriously hinder the sustainable development of agricultural, therefore agricultural production also bears unprecedented immense pressure.Domestic and international research and practice shows, plant disease is a large factor of restriction agricultural sustainable development.Be mainly manifested in the following aspects: first, plant disease directly causes grain drop in production and quality to reduce, and affects farmers' income, affects agricultural sustainable development.Secondly, although use chemical pesticide control disease to improve the output of crops, facilitate the development of agricultural, major contribution has been made in the existence for the mankind, due to its irrational use, causes very serious destruction to ecotope; Moreover causing harm of plant disease causes the increase of the population below the poverty line.
Chemical control is the effective ways controlling plant disease, advantages such as having instant effect, fungicidal spectrum is wide, cost is low, be easy to use, but long-term a large amount of use of chemical bactericide can cause the environmental pollution such as soil, air, destroy the ecological balance.Along with people are in recent years to the concern of food security and problem of environmental pollution, some chemical bactericides are strictly limited use at many developed countries and regions.People start to find a kind of to the mankind and environmentally friendly and have the new control of plant disease strategy of good control efficiency.Novel green bactericide is efficient and nontoxic, harmless, pollution-free, do not develop immunity to drugs, not only meet the demand of people to pollution-free food, and be that agriculture sustainable development provides guarantee, therefore, the research for novel green bactericide more and more comes into one's own.
Bufexamac, also known as bufexamac, Bufexamac.Chemical name is 4-n-butoxy-N-hydroxyphenylacetyl amine, also known as to fourth oxygen phenyl acetamide hydroxamic acid.English name: bufexamac, English another name: 2-(4-butoxyphenyl) acetohydroxamicacid; 2-(4-butoxyphenyl)-N-hydroxyacetamide; 4-Butoxy-N-hydroxybenzene acetamide.No. CAS: 2438-72-4, molecular formula: C
12h
17nO
3, molecular weight: 223.27, molecular structural formula is:
Bufexamac is crystallization or crystalline powder, and micro-frowziness, is soluble in dimethyl formamide, slightly soluble in methyl alcohol or ethanol, water insoluble or ether.For non-steroidal anti-inflammatory analgesics, be applicable to rheumatoid arthritis, coxitis and eczema, neurodermatitis, rheumatism etc.Preparation has tablet, creme.In animal experiment, this medicine of local topical is effective to various scytitis, special and corticosteroid unlike, do not affect the reparation of tissue, and have certain bacteriostasis.
With the antiphlogistic of bufexamac synthesis, be widely used in treatment rheumatoid arthritis, eczema and other various skin inflammation, act on quite with many cortin preparations (as Fluocet, BETAMETHASONE emulsifiable paste etc.).The preparation that this intermediate is made is nontoxic, and side effect is little.From 1973, this medicine was in succession in Britain, France, Germany, Japan and other countries listing.Until 20th century the mid-80, bufexamac emulsifiable paste remains the NSAID (non-steroidal anti-inflammatory drug) preparation of the only treatment scytitis in West Europe, is abroad used in again on toothpaste and cosmetic products recently.In addition it also has functions such as reducing body temperature, prolongation sleep, promotion natural on-off cycles of hair growth.
Bufexamac, as a kind of deacetylase inhibitor, can improve acetylation of histone level, be widely used, and to it in agricultural chemicals, the application study particularly as Fungicidal active substance is not also reported at present.
At present, there is no both at home and abroad about bufexamac the inhibiting report of agriculture pathogen.
Summary of the invention
The object of the present invention is to provide bufexamac for the preparation of control by the purposes in the bactericide of the microbial plant disease of pathogenic.The present invention, by toxicity test, demonstrates bufexamac and has good inhibit activities to plant pathogenic fungi.Bufexamac is as bactericide, and it is efficient, low toxicity, is suitable for the requirement of plant disease chemical control.
For achieving the above object, the present invention is achieved by the following technical solutions:
Bufexamac for the preparation of control by the purposes in the bactericide of the microbial plant disease of pathogenic.
Further improvement to technique scheme: described phytopathogen is Deuteromycotina fungi and Ascomycotina fungi.
Further improvement to technique scheme: in described bactericide, effective working concentration of bufexamac is 5-10mM.
Further improvement to technique scheme: described Deuteromycotina fungi comprises fusarium graminearum, botrytis cinerea, tomato wilt bacterium, blueberry shoot blight bacterium, tobacco brown spot pathogen, tomato early blight bacterium, cucumber rod spore leaf spot fungi, blueberry plan stem point shoot blight bacterium and rice blast fungus.
Further improvement to technique scheme: described Ascomycotina fungi comprises apple anthrax leaf spoting bacteria, Botryosphaeria berengeriana f. sp and blueberry limb ulcer bacteria.
Further improvement to technique scheme: when the concentration of bufexamac is 10mM in described bactericide, the inhibiting rate that bufexamac intends stem point shoot blight bacterium to botrytis cinerea and blueberry is the highest.
Further improvement to technique scheme: described plant is apple, peach, pears, tomato, blueberry, tobacco, cucumber, paddy rice, corn, wheat and soybean.
Compared with prior art, advantage of the present invention and technique effect are: chemical control is the major measure of control of plant disease always, but considerable peasant and plant protection technology personnel put undue emphasis on disease chemical control, main dependence agricultural chemicals, chemical pesticide as a shortcut, is regarded as drug effect fast by mistake, preventive effect is good, be guaranteed to cure all diseases, the gesture of this unreasonable use chemical pesticide grows in intensity, cause chemical pesticide come out so far its output and usage amount all in the gesture of rising year by year.Lavishment chemical pesticide brings " 3R " problem, namely pest pesticide resistance (Resistanee), pest is ferocious again sticks up (Resurgenee) and residue of pesticide (Residue), negative consequences caused by it is quite serious, even makes us startling.Be mainly manifested in: the 1. continuous pollution of ecotope and deterioration.2. the destruction of field ecosystem balance.3. agricultural product quality declines.4. poisoning is serious.5. pesticide resistance strengthens.Excessive applying pesticides, easily makes part pest occur pesticide resistance, not only cost of idleness, and fails timely and effective controlling disease, brings not expectable loss to agricultural production.Along with the continuous repetition of the excessive use of chemical pesticide, the continuous prolongation of service time and access times, germ produces resistance to chemical pesticide, chemical pesticide must continue to increase dosage just has control efficiency, even escalated dose is also of no avail, the Pesticide use life-span constantly shortens, and due to the shortening in Pesticide use life-span, new pesticide species certainly will to be developed.This is just in the urgent need to the research and development of the bactericide to novel green.The present invention, by Toxicity Determination, demonstrates bufexamac and has good inhibit activities to plant pathogenic fungi.Bufexamac, as bactericide, has advantage that is efficient and low toxicity, is suitable for the requirement of plant disease chemical control.
Along with people improve day by day to cry that is pollution-free, pollution-free green food, green bactericide has become the important prevention and controls in agricultural production, and many green bactericide are come out one after another, and is widely used in producing, and achieves remarkable result.And bufexamac is a kind of degradable, pollution-free, environment amenable micromolecular compound, and its pesticide resistance is poor, to non-target organism and person poultry safety, the high-quality of agricultural product and fruits and vegetables can be ensured, meet the requirement of sustainable development, its research and market application foreground wide.
Accompanying drawing explanation
Fig. 1 be in the present invention bufexamac to the bacteriostatic experiment result of fusarium graminearum;
Fig. 2 be in the present invention bufexamac to the bacteriostatic experiment result of blueberry shoot blight bacterium;
Fig. 3 be in the present invention bufexamac to the bacteriostatic experiment result of botrytis cinerea;
Fig. 4 be in the present invention bufexamac to the bacteriostatic experiment result of Botryosphaeria berengeriana f. sp;
Fig. 5 be in the present invention bufexamac to the bacteriostatic experiment result of apple anthrax leaf spoting bacteria;
Fig. 6 be in the present invention bufexamac to the bacteriostatic experiment result of tobacco brown spot pathogen;
Fig. 7 be in the present invention bufexamac to the bacteriostatic experiment result of tomato wilt bacterium;
Fig. 8 be in the present invention bufexamac to the bacteriostatic experiment result of cucumber rod spore leaf spot fungi;
Fig. 9 be in the present invention bufexamac to the bacteriostatic experiment result of blueberry limb ulcer bacteria;
Figure 10 is the bacteriostatic experiment result that in the present invention, bufexamac intends stem point shoot blight bacterium to blueberry;
Figure 11 be in the present invention bufexamac to the bacteriostatic experiment result of tomato early blight bacterium;
Figure 12 be in the present invention bufexamac to the bacteriostatic experiment result of rice blast fungus.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Embodiment 1
One, experiment material
Bufexamac is the finished product bought from Fluka company.Take 4.47g bufexamac in the dimethyl sulfoxide (DMSO) of 20ml, be made into the mother liquor of 1M, filtration sterilization 4 DEG C preservation is stand-by.
Phytopathogen used in this experiment is the bacterial classification that 4 DEG C, laboratory is preserved, and the medium of employing is potato culture (being called for short PDA).
PDA culture medium prescription: potato (peeling) 200g, glucose 20g, agar 15g, running water 1000ml, natural PH
Compound method: potato is cleaned peeling, 200g is claimed to be cut into small pieces, add water well-done (boil 20-30 minute, can be poked by glass bar), by eight layers of filtered through gauze in beaker, experimentally need to add 15-20g agar, add 20g glucose, stir, after fully dissolving, slightly cooling supplies water to 1000ml, 121 DEG C of sterilizings 20 minutes after packing, for subsequent use after cooling.
Two, experimental technique
Adopt growth rate method.
1, first by stand-by for 12 Plants pathogens, 25 DEG C of cultivation about 2d on PDA flat board.
2, the heating of PDA medium is dissolved, be cooled to 45-50 DEG C, the bufexamac adding variable concentrations respectively makes the medium (adding the dimethyl sulfoxide (DMSO) of respective volume in contrast) containing 0,2.5,5 and 10mM liquid, and pours in culture dish respectively and cool.
3, with sterile working formality, beat get circular bacterium cake (diameter 0.60cm) with each bacterial strain mycelia edge (upgrowth situation is as far as possible consistent) that 2d cultivated by card punch, choose to the dull and stereotyped central authorities of pastille with transfer needle again, then culture dish is inverted in incubator (25 DEG C) and cultivates.
4, in the growing state of the rear different time Observe and measure mycelia of process, and adopt right-angled intersection method record diameter and process data, calculate inhibiting rate and also take pictures.
Inhibiting rate (%)=(contrast hyphal diameter-process hyphal diameter)/contrast hyphal diameter × 100
Each process repetition 3 times.
Three, bufexamac is to the fungistatic effect of 12 Plants pathogen mycelial growths
Botrytis cinerea: botrytis cinerea host is extensive, can infect the various vegetables such as tomato, cucumber, eggplant, Kidney bean, green pepper, celery, asparagus lettuce, lettuce.
Fusarium graminearum: head blight is a kind of destructive disease, can cause fringe rotten, causes Severe Reduction and quality to reduce.Along with the change of global warming, cropping system and mode, wheat scab constantly spreads expansion, usually causes wheat yield, quality to reduce, and containing mycotoxin in the wheat seed infected, can cause person poultry poisoning and serious disease.This germ, except harm wheat, can also be infected the grassy weed such as the multiple gramineous crop such as barley, oat, paddy rice, corn and goose hat grass, in addition, also can infect the crops such as soybean, cotton, Ipomoea batatas.
Tobacco brown spot pathogen: alternaria altemata host range is comparatively wide, except tobacco, also can infect the various plants such as cotton, peanut, soybean, tomato, peach, Lee, wheat, cause the symptom such as spot, root rot.
Tomato wilt bacterium: the specialization of tomato wilt bacterium is extremely strong, only endangers tomato.This bacterium mainly infects flora conduit tissue, causes plant wilt by obstruction xylem material and Water Transportation.It is the soil-borne disease of a kind of serious harm quality of vegetable and output, and the Seedling Stage of tomato is all subject to infecting of wilt during Adult plant.Tomato wilt is wilt disease again, occurs serious in Lao Caipeng room.
Blueberry shoot blight bacterium: this germ belongs to plan Pestalotia is a kind of important phytopathogen, can the plant of parasitic about section more than 50, shows as tikka, rots, the symptom such as ulcer after hazard of plant, has a strong impact on the yield and quality of product.Therefore, the production of research plan pestalotia bacteria to farm-forestry crop has important economic implications.At present, the blueberry main breed in production, Ru Lanfeng, duke and blue beauty etc., all have shoot blight to occur, significantly affect its fruit yield and quality.
Apple anthrax leaf spoting bacteria: apple anthrax leaf blight is by the microbial a kind of leaf diseases of anthracnose, the kinds such as the loud, high-pitched sound of main harm apple is drawn, Jin Guan, Qiao Najin, Qin Guan, cause the apple tree leaf and fruit that falls in a large number seriously to rot, tree vigo(u)r seriously undermines, and causes next year to be had no harvest.Fuji, red magnitude kind height are disease-resistant, and the loud, high-pitched sound of morbidity is drawn and contrasted clearly in orchard with adjacent Fuji apple trees of not falling ill.Apple anthrax-bacilus leaf blight main harm blade, and cause a large amount of fallen leaves.
Blueberry intends stem point shoot blight bacterium: host mainly contains the plants such as Larch, peach, pear, Vaccinium, Jianshi maple.
Cucumber rod spore leaf spot fungi: this germ host range is extensive, and circulation way is various.Further investigation reveals that in recent years, by leaf spot 11 province, city and region's large area generations in the Shandong of China, Hebei, Liaoning, the Inner Mongol etc. of the vegetables such as the microbial cucumber of cucumber rod spore leaf spot, tomato, caused serious economic loss.
Botryosphaeria berengeriana f. sp: ring rot of apple has another name called scurf, wheel line decayed fruit is sick is the important biomolecule disaster on apple limb portion and fruit, often causes that apple limb bark is coarse, locality is downright bad and fruit rot.Ill plant fruit-setting rate is low, and cause the weak and output of tree body to reduce, even garden is ruined in total crop failure.In recent years, along with the commerial growing of susceptible kind Fuji apple, the ring rot of apple incidence of disease increases year by year, and hazard area constantly expands, and become the serious plant disease in Chinese apple production, the sustainable development of Apple Industry in serious threat.
Tomato early blight bacterium: this disease is except directly endangering stem, leaf, fruit, and tomato growth and fruit also can be suppressed to be formed, and open country and protecting field are injured all heavier, long-term underproduction 20%-30%, can reach time serious 5 both more than, even have no harvest.This cause of disease host range is extensive, except harm tomato, also can endanger the solanaceous vegetable crops such as eggplant, capsicum and potato.
Rice blast fungus: this germ not only infects paddy rice, can also infect the crops such as wheat, barley and grain, even infect the root of plant, cause serious harm to agricultural production.
Blueberry limb ulcer bacteria: cause the pathogen-Botryosphaeria of blueberry limb canker to be the universal fungi of a class, the Tree resistance caused is the great disease occurred in world wide.The host of its harm is very wide, reach 45 genus (broad-leaved tree 40 genus, coniferous tree 5 genus), serious to the harm of woods fruit tree, the symptom such as limb ulcer withered death, dry rot, gummosis of the host plants such as eucalyptus, willow, apple, pomegranate, peach, Cassia occidentalis, red bayberry, Chinese chestnut can be caused.
The raw survey of the in vitro bacteriostasis of bufexamac to 12 Plants pathogens the results are shown in Table 1.
Table 1 bufexamac is to the inhibitory action measurement result (5d) of 12 kinds of pathogen mycelial growths
Note: in test, three repetitions are established in each process, in table, data are the mean value repeated for three times.
As can be known from Table 1, bufexamac, when 10mM, all has certain inhibition to 12 Plants pathogens of Deuteromycotina and Ascomycotina.Wherein to the inhibiting rate of fusarium graminearum, the cucumber rod 8 Plants pathogens such as spore leaf spot fungi and blueberry shoot blight bacterium all more than 80%.In addition, its to botrytis cinerea and blueberry intend stem point shoot blight bacterium inhibiting rate higher, more than 90%, effectively can prevent and treat it.
To the fungistatic effect picture of 12 Plants pathogen mycelial growths as shown in figs. 1-12, when the concentration of bufexamac is at 10mM, botrytis cinerea and blueberry do not intend stem point shoot blight bacterium almost not or have little mycelial growth to bufexamac.And concerning the 10 Plants pathogens such as fusarium graminearum, tobacco brown spot pathogen and cucumber rod spore leaf spot fungi, mycelial growth is little, though or have mycelial growth, compared with the control, mycelial growth is more weak, illustrates that bufexamac serves good inhibition.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (7)
1. bufexamac for the preparation of control by the purposes in the bactericide of the microbial plant disease of pathogenic.
2. bufexamac according to claim 1 is for the preparation of preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: described phytopathogen is Deuteromycotina fungi and Ascomycotina fungi.
3. bufexamac according to claim 1 and 2 is for the preparation of preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: in described bactericide, effective working concentration of bufexamac is 5-10mM.
4. bufexamac according to claim 2 is for the preparation of preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: described Deuteromycotina fungi comprises fusarium graminearum, botrytis cinerea, tomato wilt bacterium, blueberry shoot blight bacterium, tobacco brown spot pathogen, tomato early blight bacterium, cucumber rod spore leaf spot fungi, blueberry plan stem point shoot blight bacterium and rice blast fungus.
5. bufexamac according to claim 2 is for the preparation of preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: described Ascomycotina fungi comprises apple anthrax leaf spoting bacteria, Botryosphaeria berengeriana f. sp and blueberry limb ulcer bacteria.
6. bufexamac according to claim 1 for the preparation of control by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: when in described bactericide, the concentration of bufexamac is 10mM, the inhibiting rate that bufexamac intends stem point shoot blight bacterium to botrytis cinerea and blueberry is the highest.
7. bufexamac according to claim 1 is for the preparation of preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, it is characterized in that: described plant is apple, peach, pears, tomato, blueberry, tobacco, cucumber, paddy rice, corn, wheat and soybean.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510341914.9A CN104920360B (en) | 2015-06-18 | 2015-06-18 | Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510341914.9A CN104920360B (en) | 2015-06-18 | 2015-06-18 | Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104920360A true CN104920360A (en) | 2015-09-23 |
| CN104920360B CN104920360B (en) | 2017-04-12 |
Family
ID=54108077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510341914.9A Expired - Fee Related CN104920360B (en) | 2015-06-18 | 2015-06-18 | Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104920360B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613547A (en) * | 2016-03-30 | 2016-06-01 | 青岛农业大学 | Application of glibenclamide in preparing bactericide for preventing and treating plant diseases caused by phytopathogen |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050004098A1 (en) * | 2003-03-20 | 2005-01-06 | Britten Nancy Jean | Dispersible formulation of an anti-inflammatory agent |
| WO2007061529A1 (en) * | 2005-11-18 | 2007-05-31 | Scidose Llc. | Lyophilization process and products obtained thereby |
| CN101919822A (en) * | 2010-07-16 | 2010-12-22 | 钟术光 | Tablet with improved combination properties and preparation method thereof |
| CN101987082A (en) * | 2010-07-16 | 2011-03-23 | 钟术光 | Solid preparation and preparation method thereof |
| US20140030312A1 (en) * | 2006-05-12 | 2014-01-30 | Christian Noe | Use of combination preparations comprising antifungal agents |
| CN103896810A (en) * | 2014-04-04 | 2014-07-02 | 湖南迪诺制药有限公司 | Synthesis method of bufexamac |
-
2015
- 2015-06-18 CN CN201510341914.9A patent/CN104920360B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050004098A1 (en) * | 2003-03-20 | 2005-01-06 | Britten Nancy Jean | Dispersible formulation of an anti-inflammatory agent |
| WO2007061529A1 (en) * | 2005-11-18 | 2007-05-31 | Scidose Llc. | Lyophilization process and products obtained thereby |
| US20140030312A1 (en) * | 2006-05-12 | 2014-01-30 | Christian Noe | Use of combination preparations comprising antifungal agents |
| CN101919822A (en) * | 2010-07-16 | 2010-12-22 | 钟术光 | Tablet with improved combination properties and preparation method thereof |
| CN101987082A (en) * | 2010-07-16 | 2011-03-23 | 钟术光 | Solid preparation and preparation method thereof |
| CN103896810A (en) * | 2014-04-04 | 2014-07-02 | 湖南迪诺制药有限公司 | Synthesis method of bufexamac |
Non-Patent Citations (3)
| Title |
|---|
| 孙文等: ""丁苯羟酸新的合成方法及应用"", 《精细与专用化学品》 * |
| 李庆明等: ""丁苯羟酸合成方法的改进"", 《广东药学学报》 * |
| 潘鹤林等: ""丁苯羟酸的合成"", 《合成化学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613547A (en) * | 2016-03-30 | 2016-06-01 | 青岛农业大学 | Application of glibenclamide in preparing bactericide for preventing and treating plant diseases caused by phytopathogen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104920360B (en) | 2017-04-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101851356B (en) | Shitake mushroom extract and application thereof | |
| Babu Joseph et al. | Bioefficacy of plant extracts to control Fusarium solani f. sp. melongenae incitant of brinjal wilt | |
| CN103931671A (en) | Plant seed soaking and dressing agent | |
| CN103444708A (en) | Water dispersible granule containing chitosan | |
| CN107950540B (en) | Application of 5-azacytidine in preparation of bactericide for preventing and treating plant diseases caused by phytopathogens | |
| CN110622975B (en) | Application of miconazole in preparation of bactericide for preventing and treating phytopathogen | |
| CN106942301A (en) | A kind of botanical pesticide preparation of prevention and control rice blast and preparation method thereof | |
| CN114668004B (en) | Application of perillaldehyde in preparation of bactericide for preventing and treating plant diseases | |
| CN104920367A (en) | Application of nicotinamide for preparing fungicide used for controlling plant diseases caused by pathogenic fungi | |
| CN105766906B (en) | Curcumin is being prepared for preventing by the purposes in the fungicide of the microbial plant disease of pathogenic | |
| CN104904714B (en) | Purposes of the sodium butyrate in preparing for preventing and treating the bactericide by the microbial plant disease of pathogenic | |
| CN104904715B (en) | Application of sodium valproate in preparation of fungicide used for preventing and controlling plant diseases caused by phytopathogens | |
| CN107232238A (en) | A kind of application of litsea cubeba oil on control capsicum epidemic disease | |
| CN104920360A (en) | Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen | |
| CN108157367B (en) | Application of 2, 6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple leaf | |
| CN109645007B (en) | C14H10Cl2NNaO2 is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic | |
| CN105746516B (en) | Denatonium Benzoate is being prepared for preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic | |
| CN109744246A (en) | Itraconazole is preparing the application in the fungicide for preventing and treating phytopathogen | |
| CN109645017A (en) | Sodium diethyldithiocarbamate is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic | |
| CN101919332A (en) | Method for biologically preventing and controlling leaf mold of tomatoes | |
| CN103704230A (en) | Ultralow volume liquid containing pyraclostrobin and benzamide bactericidal composition | |
| CN105746522B (en) | Indomethacin is being prepared for preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic | |
| CN110720466A (en) | Application of loperamide hydrochloride in preparation of bactericide for preventing and treating plant diseases caused by plant pathogenic bacteria | |
| CN107410318A (en) | A kind of pyridine heterocycle quaternary cationics disinfectant use in agriculture | |
| Muramoto et al. | Anaerobic soil disinfestation for controlling Fusarium wilt in strawberries |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170412 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |