CN104911103B - A kind of liquid transfer gun head for the fast quick change liquid of suspension cell - Google Patents
A kind of liquid transfer gun head for the fast quick change liquid of suspension cell Download PDFInfo
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- CN104911103B CN104911103B CN201510267443.1A CN201510267443A CN104911103B CN 104911103 B CN104911103 B CN 104911103B CN 201510267443 A CN201510267443 A CN 201510267443A CN 104911103 B CN104911103 B CN 104911103B
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- liquid
- cell
- suspension cell
- suction nozzle
- filter
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention is a kind of liquid transfer gun head for the fast quick change liquid of suspension cell, includes pipette tips body（1）, suction nozzle（2）, liquid-transfering gun interface（3）, wherein suction nozzle（2）It is installed in pipette tips body（1）One end, liquid-transfering gun interface（3）It is installed in pipette tips body（1）The other end, suction nozzle（2）End face be arc, suction nozzle（2）Inside it is equiped with filter core（21）.The present invention uses the structure with filter core, filter core can stop that cell is sucked away during nutrient solution is drawn, cell is set to stay in the container of culture, in addition, the aperture that filter core is provided with is less than the aperture of general suspension cell, prevents cell to be filtered across, in addition, in order to avoid cell blocks and sticks filter bores, the aperture of bumps is devised in filter opening.The present invention solves the problems, such as that suspension cell changes that liquid is difficult and slow, and liquid transfer gun head of the invention shortens the damage and loss changing the liquid time and reduce cell of suspension cell, reaches and suspension cell is carried out changing liquid easily and fast.
Description
Technical field
The present invention is a kind of consumptive material being related to used in suspension cell culture, and in particular to for the fast quick change liquid of suspension cell
Liquid transfer gun head, belong to the innovative technology of the liquid transfer gun head for the fast quick change liquid of suspension cell.
Background technology
Up to the present, the liquid that changes of suspension cell has two methods, the first：Cell is collected by centrifugation, plus fresh cultured
Base.Second：Inhaled after blake bottle uprightly is stood into a period of time and abandon upper strata culture medium, equivalent changes fresh culture medium.This two
All there is the shortcomings that certain in kind method, method of the first centrifugation has certain infringement to cell, especially more fragile to some
Cell, the less cell centrifuged deposit of some cell concentrations is rare, and cell is easily lost when abandoning supernatant, also operates ratio
Cumbersome, the time of consumption is very long, and the accurate experiment of some time requirements, such as cell administration are tested, and some medicines are taken the photograph
Take and the pharmacokinetics cell model of outer row is, it is necessary to be collected into the accumulation medicine of Each point in time, to Each point in time
Requirement it is more accurate experimental error it is larger.The method for changing liquid is collected by centrifugation according to cell, has in time certain
Error.Change liquid method and can lose a certain amount of cell for second, impact cell slow to the speed of growth once and more precious compared with
Greatly, and uprightly standing allows cell precipitation to need to consume the regular hour, requires that higher experiment does not apply to time point.Therefore
Developing a kind of there is quick and noninvasive liquid method of changing to be particularly important to suspension cell.Liquid-transfering gun is that laboratory one kind is common
Miniature instrument, quantifying for liquid is measured and the quick removal important role of waste liquid, if it is possible to for suspension cell
Change liquid, then will solve suspension cell and change the difficult slow problem of liquid, existing market does not have such to suspend with applying mechanically with removing
The pipette tips of cell culture fluid.
The content of the invention
A kind of being used for for fast quick change liquid of achievable suspension cell is provided it is an object of the invention to consider above mentioned problem
The liquid transfer gun head of the fast quick change liquid of suspension cell.The present invention is simple to operate, convenient and practical.
The technical scheme is that：The liquid transfer gun head for the fast quick change liquid of suspension cell of the present invention, includes pipette tips
Body, suction nozzle, liquid-transfering gun interface, wherein suction nozzle are installed in one end of pipette tips body, and liquid-transfering gun interface is installed in pipette tips body
The other end, the end face of suction nozzle is arc, and filter core is equiped with suction nozzle.
The liquid transfer gun head for the fast quick change liquid of suspension cell of the present invention, using the structure with filter core, drawing nutrient solution
During filter core can stop that cell is sucked away, cell is stayed in the container of culture, in addition, the aperture that filter core is provided with is less than
The aperture of general suspension cell, prevents cell to be filtered across, in addition, in order to avoid cell blocks and sticks filter bores, in filter opening
Devise the aperture of bumps.The present invention solves the problems, such as that suspension cell changes that liquid is difficult and slow, and liquid transfer gun head of the invention shortens
The damage and loss changing the liquid time and reduce cell of suspension cell, reach and suspension cell are carried out changing liquid easily and fast.This
Invention is a kind of ingenious in design, function admirable, the convenient and practical liquid transfer gun head for the fast quick change liquid of suspension cell.Present invention behaviour
Make simply, it is convenient and practical.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 looks up enlarged drawing for filter core of the present invention.
Embodiment
Below in conjunction with the accompanying drawings, the embodiment of the present invention is further elaborated.
The structural representation of the present invention is as shown in figure 1, the liquid transfer gun head for the fast quick change liquid of suspension cell of the present invention, bag
Pipette tips body 1, suction nozzle 2, liquid-transfering gun interface 3 are included, wherein suction nozzle 2 is installed in one end of pipette tips body 1, and liquid-transfering gun interface 3 fills
The other end of pipette tips body 1 is located at, the end face of suction nozzle 2 is arc, and filter core 21 is equiped with suction nozzle 2.The filter cartridge construction can be with
Stop that the suspension cell of culture is sucked away with culture medium.
The filter core 21 of installing includes some convex surfaces 211 and some concave surfaces 212 in above-mentioned suction nozzle 2, and wherein convex surface 211 is not set
Filter opening, concave surface 212 sets filter opening 213, and in the present embodiment, the bottom surface and side of concave surface 212 all set filter opening 213, filter core 21
End face is arc.
In the present embodiment, the pore size of the filter opening 213 that above-mentioned concave surface 212 is set is can prevent cell obstruction to be advisable.
In the present embodiment, the aperture for being smaller than cell between above-mentioned each convex surface 211.Above-mentioned each convex surface 211 it
Between spacing can have different specifications, according to the liquid transfer gun head of the different optional different size of the diameter of cell.The present invention
The concaveconvex structure of the filter core can not only stop that the suspension cell of culture is sucked away with culture medium, and concavo-convex filter opening and suction
The arcuate structure of head filter core is less useful for cell and shows to stick in suction nozzle, and filter opening is not set in convex, and filter opening is set in recess, each
Maximum between the part of protrusion is smaller than the diameter of cell, prevent cell enter concave surface block concave surface aperture can be according to thin
The diameter of born of the same parents come select application specification.
The material of above-mentioned filter core 21 is glass fibre or plastics.In the present embodiment, the material of above-mentioned filter core 21 is glass fibers
Dimension.
In the present embodiment, the aperture for the filter opening 213 that above-mentioned concave surface 212 is set is completed with the mode of laser boring.Filter core
Male and fomale(M＆F) can use the modelling prepared.
The liquid transfer gun head of the present invention is mainly used in the fast quick change liquid of suspension cell, and can be used for other similar suspensions
The liquid of liquid and emulsion etc. pipettes.This pipette tips need to only support the use with the liquid-transfering gun of laboratory routine.The present invention can
Enough by cell filtration, it can prevent cell from blocking filter opening and causing liquid not siphon away again, quickly culture medium can be siphoned away and will hanged
Floating cell stays in bottom of bottle, solves the problem that suspension cell changes liquid inconvenience.
Claims (2)
1. a kind of liquid transfer gun head for the fast quick change liquid of suspension cell, it is characterised in that include pipette tips body（1）, suction nozzle（2）、
Liquid-transfering gun interface（3）, wherein suction nozzle（2）It is installed in pipette tips body（1）One end, liquid-transfering gun interface（3）It is installed in pipette tips body
（1）The other end, suction nozzle（2）End face be arc, suction nozzle（2）Inside it is equiped with filter core（21）；Above-mentioned suction nozzle（2）The filter of interior installing
Core（21）Include some convex surfaces（211）And concave surface（212）, convex surface（211）Do not set filter opening, concave surface（212）Bottom surface and side
Filter opening is all set（213）；The concave surface of filter core（212）The filter opening being provided with（213）Aperture be less than the aperture of suspension cell, prevent thin
Born of the same parents are filtered across；Above-mentioned each convex surface（211）Between the aperture for being smaller than suspension cell；Above-mentioned concave surface（212）The filter of setting
Hole（213）Use laser boring.
2. the liquid transfer gun head according to claim 1 for the fast quick change liquid of suspension cell, it is characterised in that above-mentioned filter core
（21）Material be glass fibre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510267443.1A CN104911103B (en) | 2015-05-25 | 2015-05-25 | A kind of liquid transfer gun head for the fast quick change liquid of suspension cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510267443.1A CN104911103B (en) | 2015-05-25 | 2015-05-25 | A kind of liquid transfer gun head for the fast quick change liquid of suspension cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104911103A CN104911103A (en) | 2015-09-16 |
| CN104911103B true CN104911103B (en) | 2018-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510267443.1A Active CN104911103B (en) | 2015-05-25 | 2015-05-25 | A kind of liquid transfer gun head for the fast quick change liquid of suspension cell |
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| CN (1) | CN104911103B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410714B (en) * | 2018-03-16 | 2021-05-04 | 苏州亚通生物医疗科技有限公司 | Cell mixing pipette |
| KR102237531B1 (en) * | 2019-01-08 | 2021-04-06 | 재단법인대구경북과학기술원 | Pipette tip for preventing loss of sample |
| CN116037240B (en) * | 2022-11-25 | 2023-08-01 | 杭州皓丰生物技术有限公司 | A liquid-transfering gun head for suspending quick liquid of cell |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7785466B1 (en) * | 1996-04-10 | 2010-08-31 | Smith James C | Membrane filtered pipette tip |
| CN202983705U (en) * | 2012-11-06 | 2013-06-12 | 无锡耐思生物科技有限公司 | Pipette gun head structure with filter element |
| CN203065472U (en) * | 2013-02-04 | 2013-07-17 | 中国热带农业科学院橡胶研究所 | Spearhead |
| CN203625369U (en) * | 2013-12-17 | 2014-06-04 | 朱廷准 | Filter gun head of pipette for cell sphere experiment |
| CN204093472U (en) * | 2014-10-15 | 2015-01-14 | 浙江硕华医用塑料有限公司 | Disposable liquid transfer gun head |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012103214A2 (en) * | 2011-01-26 | 2012-08-02 | Molecular Bioproducts, Inc. | Magnetic pipette tip |
| CN203540173U (en) * | 2013-09-30 | 2014-04-16 | 虞小生 | Anti-blocking filter |
-
2015
- 2015-05-25 CN CN201510267443.1A patent/CN104911103B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7785466B1 (en) * | 1996-04-10 | 2010-08-31 | Smith James C | Membrane filtered pipette tip |
| CN202983705U (en) * | 2012-11-06 | 2013-06-12 | 无锡耐思生物科技有限公司 | Pipette gun head structure with filter element |
| CN203065472U (en) * | 2013-02-04 | 2013-07-17 | 中国热带农业科学院橡胶研究所 | Spearhead |
| CN203625369U (en) * | 2013-12-17 | 2014-06-04 | 朱廷准 | Filter gun head of pipette for cell sphere experiment |
| CN204093472U (en) * | 2014-10-15 | 2015-01-14 | 浙江硕华医用塑料有限公司 | Disposable liquid transfer gun head |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104911103A (en) | 2015-09-16 |
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