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CN104904962A - Complex fermented gynostemma pentaphylla type Chinese herbal medicine health tea - Google Patents

Complex fermented gynostemma pentaphylla type Chinese herbal medicine health tea Download PDF

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Publication number
CN104904962A
CN104904962A CN201510393057.7A CN201510393057A CN104904962A CN 104904962 A CN104904962 A CN 104904962A CN 201510393057 A CN201510393057 A CN 201510393057A CN 104904962 A CN104904962 A CN 104904962A
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gynostemma pentaphylla
chinese herbal
herbal medicine
cold tea
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王金波
董静静
侯文燕
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Qingdao Jiarui Biological Technology Co Ltd
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Qingdao Jiarui Biological Technology Co Ltd
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Abstract

The invention discloses gynostemma pentaphylla herbal tea which has the effects of traditional herbal tea of clearing heat and removing toxicity, clearing damp, relieving summer heat and decreasing internal heat, and also has the effects of resisting fatigue, promoting metabolism, boosting immunity, resisting ageing, decreasing blood lipid, protecting liver and dredging gallbladder, and tonifying liver and nourishing kidney. The health herbal tea is prepared from the following Chinese herbal medicines in parts by mass: 30 to 40 parts of gynostemma pentaphylla, 10 to 20 parts of honeysuckle, 10 to 20 parts of liquorice, 5 to 10 parts of chrysanthemum, 10 to 20 parts of lycium barbarum, 8 to 12 parts of mulberry, 5 to 10 parts of acanthopanax, 5 to 10 parts of rhodiola rosea, 3 to 5 parts of angelica sinensis, 3 to 5 parts of codonopsis pilosula, 3 to 5 parts of astragalus membranaceus, 2 to 5 parts of houttuynia cordata, 5 to 10 parts of leaf of paniculate microcos, 2 to 5 parts of plantain herb, 2 to 3 parts of polygala tenuifolia, 1 to 3 parts of sealwort, and 1 to 3 parts of polygonum multiflorum. According to the gynostemma pentaphylla herbal tea, the production process in combination of ultrasonic aided secondary extracting and microbial fermentation is adopted, so that the extracting rate of active substances is high, and the effect and palatability of the tea are good; in addition, the requirements on devices are small; mass industrial production can be conveniently carried out.

Description

A kind of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea
Technical field
The present invention relates to a kind of with the formula of the Chinese herbal medicines such as the gynostemma pentaphylla health-care cold tea that is raw material and preparation method, belong to herbal health care field of food.
Background technology
Current China beverage market is based on soda, fruit drink and functional beverage, meet consumer demand to a certain extent, but along with the raising of living standards of the people, particularly with the quickening pace of modern life and the increase of life stress, more consumer yearns for the pure natural beverage with health care good mouthfeel.Cold tea is more and more subject to the favor of consumers in general as the health-preserving beverage of the traditional daily life of China, tradition of drinking cold tea among the people is long-standing, records according to according to Compendium of Material Medica: the Chinese herbal medicines such as the primary raw material honeysuckle of cold tea, lophatherum gracile, mulberry leaf, dandelion, chrysanthemum, Radix Glycyrrhizae and peppermint have clearing heat and detoxicating, clearing damp, health-care effects such as falling fire of relieving summer heat.Cold tea huge number in current China market, function according to cold tea can be divided into traditional herb tea and health-care cold tea, the major function of traditional herb tea is based on clearing away summerheat, clearing away the lungheat and moisturizing, the current cold tea of China belongs to traditional herb tea more, as WANGLAOJI LIANGCHA, that its major function is clearing away summerheat, clearing damp disappears is stagnant, promote the production of body fluid to quench thirst; Health-care cold tea is different from traditional herb tea, is applicable to all healthy populations, can plays effect of clearing away summerheat, clearing away the lungheat and moisturizing, also there is specific health care, become the development priority of cold tea gradually, particularly pure vegetable Chinese herbal medicine health-care cold tea, deeply favor by consumers in general.
Modern study shows that increasing Chinese herbal medicine can be applicable in the production of health-care cold tea, makes the health-care efficacy of health-care cold tea more and more abundanter.In health-care cold tea product of the present invention, main Chinese herbal medicine comprises gynostemma pentaphylla, honeysuckle, Radix Glycyrrhizae, chrysanthemum, matrimony vine, mulberries, the Radix Astragali etc.The brief introduction of part herbal raw material is as follows:
Gynostemma pentaphylla ( gynostemma pentaphyllum), have another name called seven leaf courages, Pentapanax leschenaultii, gynostemma pentaphyllum makino etc., be Curcurbitaceae gynostemma pentaphyllum genus herbaceous perennial vine plant, can be used for the precious Chinese medicine material of health food, be listed in top grade in Shennong's Herbal.Composition Study shows, gynostemma pentaphylla contains multiple nutritional components and the functional health factors such as gypenoside, flavones, polysaccharide, vitamin, organic acid, alkaloid, protein.The medical treatment of gynostemma pentaphylla and medicine healthy sofa are worth oneself and studies have reported that, clinically to Tumor suppression, prevent aging, reduce blood fat, develop immunitypty, sedation-analgesia, cardioprotection and liver etc. all have definite curative effect.In recent years, research shows that gynostemma pentaphylla has multiple physiologically active, and gynostemma pentaphylla has antifatigue, reduces lipid peroxide, promotes cell metabolism and the clinical treatment deficiency of vital energy, white hair and prevents and treats disease of old people, anticancer aspect has positive role.Gynostemma pentaphylla has wide exploitation prospect.Because it has the primary efficacy of ginseng, again without the side effect of ginseng, therefore cause the great attention of domestic and international expert.But send out, still based on Chinese herbal medicine compatibility agent because food industries that the reason in effect, production technology etc. not yet realizes scale melts at China's gynostemma pentaphylla product at present;
Honeysuckle since ancient times just with medicinal and famous, Shennong's Herbal record " honeysuckle taste cold in nature is sweet, has effect that is clearing heat and detoxicating, cooling blood and removing stasis, cure mainly affection of exogenous wind-heat, seasonal febrile diseases originally, sore virus, red and swollen heat pain, passing stool with pus and blood " etc.Honeysuckle is recorded and has the health-care efficacy of " clothes are made light of one's life by commiting suicide for a long time, promote longevity " in Compendium of Material Medica.Radix Glycyrrhizae is a kind of traditional Chinese medicine, of many uses, and have saying of " ten side nine grass ", " Sheng Nong's herbal classic " is classified as top grade, and well-known doctor's Tao Hongjing is referred to as " state is old " and " king of many medicines ".Radix Glycyrrhizae taste is put down, and enter spleen, stomach, lung channel, invigorate the spleen and benefit qi, cough-relieving apophlegmatic, clearing heat and detoxicating, qualcomm meridian, sharp vim and vigour, the function of coordinating the drug actions of a prescription, have the title of " gentle man ".With the medicinal extract of Radix Glycyrrhizae processing, treatment tracheitis, pharyngitis, urethritis, diarrhoea, rheumatoid arthritis etc.In recent years, the extract of Radix Glycyrrhizae makes an addition in the food such as beer, beverage, candy, soy sauce.Chrysanthemum taste is sweet, bitter, and cold nature is nontoxic, has loose wind heat-clearing, flat liver improving eyesight, kobadrin, effect of eliminating inflammation and expelling toxin.Chrysanthemum water decoction and various extract, have the effect of obvious hemangiectasis, improve significantly to hypoferric anemia.Water decoction has inhibitory action to multiple pathogenic coccus and bacillus, multiple pathogenic dermatophyte.
Matrimony vine is the Chinese herbal medicine of China's healthcare field most using value and application prospect, the main medical species of current matrimony vine be lycium barbarum ( lycium barbarum L.), matrimony vine whole body is precious, records " nourishing the liver, improving eyesight, hard smart bone, goes fatigue, and easy color, bleaches, and improving eyesight is calmed the nerves, and makes us long-lived for matrimony vine, kidney-tonifying sperm-generating " according to Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.It is rich in various active material, and LBP-X (LBP) is one of its main active, have increase leukocyte activity, promote that liver cell is newborn, have immunological regulation, antitumor, protect the liver, step-down, hypoglycemic anti-ageing multiple effect of waiting for a long time;
Mulberries have another name called sorosis, Sang Zi, and be the fruit ear of perennial woody plant mulberry, the Regional Distribution of planting from south to north in China is all very extensive.Mulberries are nutritious, also have good value medical health care.Moisture content 84.71% in mulberries, crude protein 0.36%, invert sugar 9.16%, ash content 0.66%, crude fibre 0.91%, in addition also containing malic acid, V b1, V b2, thiamine, riboflavin, ascorbic acid and carrotene etc.Record according to many medical history records, mulberries taste is sweet, cold in nature, has and promotes the production of body fluid to quench thirst, liver-kidney tonifying, and relieve dizziness, high fever, infantile convulsions, epilepsy, etc. taste liquid, black invention order, sharp joint, the effect of relieving the effect of alcohol and eliminating disease and prolong life.Modern medicine carries out pharmacology to mulberries, the result of toxicity test also shows, it can improve the activity of animal body endoenzyme, suppresses the generation of harmful substance, strengthens resistivity, delays or prevent the generation of disease, and improves the immunologic function etc. of human body.Mulberries are one of agricultural product being classified as " being food and medicine " by the Ministry of Public Health.
Summary of the invention
One is the object of the present invention is to provide both to have traditional herb tea effect, there is again the gynostemma pentaphylla Chinese herbal medicine health care cold tea of the herbal health care effects such as gynostemma pentaphylla, matrimony vine, mulberries, the Radix Astragali, this product is applicable to all groups, not only there is clearing heat and detoxicating, clearing damp, effects such as falling fire of relieving summer heat, also there is antifatigue, enhance metabolism, develop immunitypty, anti-ageing, reducing blood lipid, hepatic cholagogic, the effect such as liver-kidney tonifying.
The invention provides a kind of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, it is characterized in that: health-care cold tea is made up of the Chinese herbal medicine of following mass fraction: gynostemma pentaphylla 30 ~ 40 parts, honeysuckle 10 ~ 20 parts, 10 ~ 20 parts, Radix Glycyrrhizae, chrysanthemum 5 ~ 10 parts, matrimony vine 10 ~ 20 parts, mulberries 8 ~ 12 parts, wilsonii 5 ~ 10 parts, rhodiola root 5 ~ 10 parts, Radix Angelicae Sinensis 3 ~ 5 parts, Radix Codonopsis 3 ~ 5 parts, the Radix Astragali 3 ~ 5 parts, cordate houttuynia 2 ~ 5 parts, buzhaye 5 ~ 10 parts, Asiatic plantain 2 ~ 5 parts, polygala root 2 ~ 3 parts, sealwort 1 ~ 3 part, the fleece-flower root 1 ~ 3 part.
For achieving the above object, the present invention adopts following technical scheme:
The preparation method of gynostemma pentaphylla Chinese herbal medicine health care cold tea, is characterized in that: specifically comprise the following steps:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, is chopped into 2 ~ 3mm;
(2) weigh: according to each raw material of gynostemma pentaphylla Chinese herbal medicine health care cold tea formula for a product precise;
(3) clean: clean traditional Chinese medicinal material raw materials 3 times, to guarantee product health with the drinking water of flowing;
(4) Ultrasonic Wave-Assisted Extraction: join in herbal raw material by the ratio of leaching liquor pure water 30 ~ 50:1 in mass ratio, under ultrasonic assistant effect, keeps slight boiling condition 30min;
(5) fermentable: after ultrasonic-leaching terminates, with glucose adjustment zymotic fluid initial total sugar content 6 ~ 8%, add 0.15% ammonium sulfate, 0.10% potassium dihydrogen phosphate, inoculation saccharomyces cerevisiae and bacillus, inoculative proportion is respectively 3 ~ 5%, 1 ~ 2%, and ferment 12 ~ 24h under agitation;
(6) ultrasonic assistant secondary lixiviate: after fermentation ends under ultrasonic assistant effect secondary lixiviate 10 ~ 20min, extraction temperature controls at 60 ~ 80 DEG C;
(7) centrifugal: centrifugal supernatant, filter residue adds 3 ~ 5 times of pure water, centrifugal, merges supernatant and is leaching liquor;
(8) prepare burden: add in 1 ~ 1.5g/L xylitol, 1 ~ 2g/L calcium lactate, 5 ~ 10g/L citric acid, 10 ~ 20g/L oligoisomaltose, 50mg/L calgon, 50mg/L Vc, 50mg/L potassium sorbate ratio in leaching liquor and prepare burden and stir;
(9) micro-pore-film filtration: namely obtain gynostemma pentaphylla Chinese herbal medicine health care cold tea finished product with the micro-pore-film filtration leaching liquor that aperture is 1.5 μm;
(10) sterile filling sealing: filling and sealing in sterile filling production line.
The invention provides a kind of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, product color uniformity, in even brown color liquid; Have the intrinsic flavour of cold tea product and fragrance, sense of taste is coordinated soft, sour and sweet palatability, free from extraneous odour.Soluble solid >=8g/100g in cold tea product; PH4.5 ~ 6.5; Total arsenic (in As)≤0.2m g/L; Plumbous (in Pb)≤0.2mg/L; Total plate count≤100cfu/m L, coliform≤6 MPN/100mL, mould≤10cfu/mL, yeast≤10cfu/mL, detects without pathogenic bacteria.Health-care cold tea prepared by the technique that the present invention adopts Ultrasonic Wave-Assisted Extraction and fermentable to combine, in product, sugared content reaches 695.71 ± 6.35mg/L, functional oligosaccharide content accounts for more than 60% ratio, and be rich in microbial polysaccharide, saponin content reaches 121.34 ± 4.11mg/100mL, be significantly higher than product prepared by simple Ultrasonic Wave-Assisted Extraction or microorganism fermentation process, achieve good effect.
The invention provides a kind of formula and processing technology of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, wherein gynostemma pentaphylla Chinese herbal medicine health care cold tea product there is clearing heat and detoxicating, clearing damp, relieve summer heat fall fire, antifatigue, enhance metabolism, develop immunitypty, anti-ageing, reducing blood lipid, hepatic cholagogic, the effect such as liver-kidney tonifying, be suitable for all groups and drink.Transformation Application of the present invention not only can change the consumption result of antipyretic beverage, meet the demand of the people to health-care food, be of value to national health, and high-valuedly can make full use of gynostemma pentaphylla resource, increase the income of the gynostemma pentaphylla growing area people, drive farmer richness.
The invention provides a kind of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, it is advantageous that: one is that the technique adopting ultrasonic assistant second extraction and fermentable to combine extracts Chinese herbal medicine active material, improve the recovery rate of active material, improve the nutritive value of gynostemma pentaphylla Chinese herbal medicine health care cold tea; Two be that product not only has that traditional herb tea is clearing heat and detoxicating, clearing damp, effects such as falling fire of relieving summer heat, also there is the antifatigue of the Chinese herbal medicines such as gynostemma pentaphylla, enhance metabolism, develop immunitypty, anti-ageing, reducing blood lipid, hepatic cholagogic, the effect such as liver-kidney tonifying; Three is with the addition of the batchings such as xylitol, calcium lactate, citric acid, oligoisomaltose, calgon, Vc, potassium sorbate in gynostemma pentaphylla Chinese herbal medicine health care cold tea product, product good palatability, extend the shelf life of product, and instead of sucrose with oligoisomaltose, make health-care cold tea product be suitable for comprising all groups of diabetic; Four is substitute traditional bus sterilization with micro-pore-film filtration, and sterilization effect is thorough, and avoid the nutriment loss that high temperature brings, product clarity is high; Five is that production technology recovery rate is high, relatively low to equipment requirement, is convenient to large-scale industrial production.
Detailed description of the invention
embodiment 1:
Prepare gynostemma pentaphylla Chinese herbal medicine health care cold tea with Chinese herbal medicines such as gynostemma pentaphylla, its concrete production technology is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, is chopped into 2 ~ 3mm;
(2) weigh: by gynostemma pentaphylla 35 parts, honeysuckle 15 parts, 15 parts, Radix Glycyrrhizae, chrysanthemum 8 parts, matrimony vine 15 parts, mulberries 10 parts, wilsonii 8 parts, rhodiola root 8 parts, Radix Angelicae Sinensis 4 parts, Radix Codonopsis 4 parts, the Radix Astragali 4 parts, cordate houttuynia 3 parts, buzhaye 7 parts, Asiatic plantain 3 parts, polygala root 2.5 parts, sealwort 2 parts, each raw material of the fleece-flower root 2 parts formula precise;
(3) clean: clean traditional Chinese medicinal material raw materials 3 times, to guarantee product health with the drinking water of flowing;
(4) Ultrasonic Wave-Assisted Extraction: join in herbal raw material by the ratio of leaching liquor pure water 40:1 in mass ratio, under ultrasonic assistant effect, keeps slight boiling condition 30min;
(5) fermentable: after ultrasonic-leaching terminates, with glucose adjustment zymotic fluid initial total sugar content 7%, add 0.15% ammonium sulfate, 0.10% potassium dihydrogen phosphate, inoculation saccharomyces cerevisiae and bacillus, inoculative proportion is respectively 4%, 1.5%, and ferment 18h under agitation;
(6) ultrasonic assistant secondary lixiviate: after fermentation ends under ultrasonic assistant effect secondary lixiviate 15min, extraction temperature controls at 60 ~ 80 DEG C;
(7) centrifugal: centrifugal supernatant, filter residue adds 4 times of pure water, centrifugal, merges supernatant and is leaching liquor;
(8) prepare burden: add in proportion in leaching liquor and prepare burden and stir: 1g/L xylitol, 2g/L calcium lactate, 10g/L citric acid, 10g/L oligoisomaltose, 50mg/L calgon, 50mg/L Vc, 50mg/L potassium sorbate;
(9) micro-pore-film filtration: namely obtain gynostemma pentaphylla Chinese herbal medicine health care cold tea finished product with the micro-pore-film filtration leaching liquor that aperture is 1.5 μm;
(10) sterile filling sealing: filling and sealing in sterile filling production line.
embodiment 2:
Prepare gynostemma pentaphylla Chinese herbal medicine health care cold tea with Chinese herbal medicines such as gynostemma pentaphylla, its concrete production technology is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, is chopped into 2 ~ 3mm;
(2) weigh: by gynostemma pentaphylla 30 parts, honeysuckle 20 parts, 10 parts, Radix Glycyrrhizae, chrysanthemum 10 parts, matrimony vine 10 parts, mulberries 12 parts, wilsonii 5 parts, rhodiola root 10 parts, Radix Angelicae Sinensis 3 parts, Radix Codonopsis 5 parts, the Radix Astragali 3 parts, cordate houttuynia 5 parts, buzhaye 5 parts, Asiatic plantain 5 parts, polygala root 2 parts, sealwort 3 parts, each raw material of the fleece-flower root 1 part formula precise;
(3) clean: clean traditional Chinese medicinal material raw materials 3 times, to guarantee product health with the drinking water of flowing;
(4) Ultrasonic Wave-Assisted Extraction: join in herbal raw material by the ratio of leaching liquor pure water 30:1 in mass ratio, under ultrasonic assistant effect, keeps slight boiling condition 30min;
(5) fermentable: after ultrasonic-leaching terminates, with glucose adjustment zymotic fluid initial total sugar content 6%, add 0.15% ammonium sulfate, 0.10% potassium dihydrogen phosphate, inoculation saccharomyces cerevisiae and bacillus, inoculative proportion is respectively 3%, 1%, and ferment 24h under agitation;
(6) ultrasonic assistant secondary lixiviate: after fermentation ends under ultrasonic assistant effect secondary lixiviate 20min, extraction temperature controls at 60 ~ 80 DEG C;
(7) centrifugal: centrifugal supernatant, filter residue adds 3 times of pure water, centrifugal, merges supernatant and is leaching liquor;
(8) prepare burden: add in proportion in leaching liquor and prepare burden and stir: 1.2g/L xylitol, 1.5g/L calcium lactate, 8g/L citric acid, 15g/L oligoisomaltose, 50mg/L calgon, 50mg/L Vc, 50mg/L potassium sorbate;
(9) micro-pore-film filtration: namely obtain gynostemma pentaphylla Chinese herbal medicine health care cold tea finished product with the micro-pore-film filtration leaching liquor that aperture is 1.5 μm;
(10) sterile filling sealing: filling and sealing in sterile filling production line.
embodiment 3:
Prepare gynostemma pentaphylla Chinese herbal medicine health care cold tea with Chinese herbal medicines such as gynostemma pentaphylla, its concrete production technology is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, is chopped into 2 ~ 3mm;
(2) weigh: by gynostemma pentaphylla 40 parts, honeysuckle 10 parts, 20 parts, Radix Glycyrrhizae, chrysanthemum 5 parts, matrimony vine 20 parts, mulberries 8 parts, wilsonii 10 parts, rhodiola root 5 parts, Radix Angelicae Sinensis 5 parts, Radix Codonopsis 3 parts, the Radix Astragali 5 parts, cordate houttuynia 2 parts, buzhaye 10 parts, Asiatic plantain 2 parts, polygala root 3 parts, sealwort 1 part, each raw material of the fleece-flower root 3 parts formula precise;
(3) clean: clean traditional Chinese medicinal material raw materials 3 times, to guarantee product health with the drinking water of flowing;
(4) Ultrasonic Wave-Assisted Extraction: join in herbal raw material by the ratio of leaching liquor pure water 50:1 in mass ratio, under ultrasonic assistant effect, keeps slight boiling condition 30min;
(5) fermentable: after ultrasonic-leaching terminates, with glucose adjustment zymotic fluid initial total sugar content 8%, add 0.15% ammonium sulfate, 0.10% potassium dihydrogen phosphate, inoculation saccharomyces cerevisiae and bacillus, inoculative proportion is respectively 5%, 2%, and ferment 12h under agitation;
(6) ultrasonic assistant secondary lixiviate: after fermentation ends under ultrasonic assistant effect secondary lixiviate 20min, extraction temperature controls at 60 ~ 80 DEG C;
(7) centrifugal: centrifugal supernatant, filter residue adds 5 times of pure water, centrifugal, merges supernatant and is leaching liquor;
(8) prepare burden: add in proportion in leaching liquor and prepare burden and stir: 1.5g/L xylitol, 1/L calcium lactate, 5g/L citric acid, 20g/L oligoisomaltose, 50mg/L calgon, 50mg/L Vc, 50mg/L potassium sorbate;
(9) micro-pore-film filtration: namely obtain gynostemma pentaphylla Chinese herbal medicine health care cold tea finished product with the micro-pore-film filtration leaching liquor that aperture is 1.5 μm;
(10) sterile filling sealing: filling and sealing in sterile filling production line.
The invention provides a kind of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, different Chinese herbal medicine nature and flavor are different, effect also exists huge difference, complicated interaction relationship is there is after different Chinese herbal medicine combinations, and proportioning between Chinese herbal medicine is closer, different ratio also will make monarch relation that substantial change occurs, thus make the relation generation material alterations of composite Chinese herbal.The application, and there is complicated interaction relationship to each other by Chinese herbal medicines such as gynostemma pentaphylla and honeysuckle, Radix Glycyrrhizae, chrysanthemum, matrimony vine, mulberries, the Radix Astragali, cordate houttuynia, buzhaye, Asiatic plantain, sealworts, rational proportion, achieve unforeseeable technique effect, make the relation generation material alterations of gynostemma pentaphylla Chinese herbal medicine health care cold tea, the TAC of gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea reaches 323.58 ± 10.44U/mL, and change Chinese herbal medicine proportioning or Chinese herbal medicine composition can reduce the oxidation resistance of health-care cold tea.Below by way of description of test the application health-care cold tea anti-fatigue effect:
Experimental animal: healthy Kunming Strains of Mouse, male, body weight 18 ~ 22g;
Animal grouping and process: with national standard spray tooth class animal drying word material adaptability feed 2 days after (room temperature controls at about 25 DEG C), mouse is divided into 4 groups at random: control group, test group 1(embodiment 1), test group 2(embodiment 2) and test group 3(embodiment 3), often organize 10, sub-cage rearing.At set time every morning section health-care cold tea gastric infusion respectively, control group is filled with the common bread gastric infusion of equal-volume, continuous 12d.Fasting 2 h (can freely drink water) after last administration, the water went swimming then each group of mouse being placed in 22 ± 1 DEG C exhausts to power, record swimming time.Sink more than also floating for 10 seconds after water with mouse, and put and can not complete righting reflex in the plane and exhaust criterion as power.After swimming terminates, each group of mouse is placed in dry mouse cage and recovers 24 h, pluck eyeball Bian blood immediately for subsequent use;
Serum Indexes measures: adopt industry standard methods to measure;
Result of the test:
(1) health-care cold tea is on the impact of mouse swimming time
Table 1 respectively group mouse is swum to the time that power exhausts (n=10)
Note: * represents that otherness is remarkable compared with control group, * p<0.05, * p<0.01
(2) health-care cold tea is on the impact of Biochemical Indices In Serum
The comparison of mice serum biochemical indicator respectively organized by table 2
Note: * represents that otherness is remarkable compared with control group, * p<0.05, * p<0.01
From above-mentioned result of the test, the application's health-care cold tea can be swum to the time that power exhausts by significant prolongation mouse compared with blank group, improves mice serum biochemical indicator, statistical analysis significant difference; Cold tea Chinese herbal medicine composition or the remarkable antifatigue effect reducing health-care cold tea of extraction process meeting is changed in test.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.

Claims (1)

1. a gynostemma pentaphylla Composite fermentation type Chinese herbal medicine health care cold tea, is characterized in that preparing as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, is chopped into 2 ~ 3mm;
(2) weigh: according to gynostemma pentaphylla 30 ~ 40 parts, honeysuckle 10 ~ 20 parts, 10 ~ 20 parts, Radix Glycyrrhizae, chrysanthemum 5 ~ 10 parts, matrimony vine 10 ~ 20 parts, mulberries 8 ~ 12 parts, wilsonii 5 ~ 10 parts, rhodiola root 5 ~ 10 parts, Radix Angelicae Sinensis 3 ~ 5 parts, Radix Codonopsis 3 ~ 5 parts, the Radix Astragali 3 ~ 5 parts, cordate houttuynia 2 ~ 5 parts, buzhaye 5 ~ 10 parts, Asiatic plantain 2 ~ 5 parts, polygala root 2 ~ 3 parts, sealwort 1 ~ 3 part, each raw material of the fleece-flower root 1 ~ 3 part formula precise;
(3) clean: clean traditional Chinese medicinal material raw materials 3 times, to guarantee product health with the drinking water of flowing;
(4) Ultrasonic Wave-Assisted Extraction: join in herbal raw material by the ratio of leaching liquor pure water 30 ~ 50:1 in mass ratio, under ultrasonic assistant effect, keeps slight boiling condition 30min;
(5) fermentable: after ultrasonic-leaching terminates, with glucose adjustment zymotic fluid initial total sugar content 6 ~ 8%, add 0.15% ammonium sulfate, 0.10% potassium dihydrogen phosphate, inoculation saccharomyces cerevisiae and bacillus, inoculative proportion is respectively 3 ~ 5%, 1 ~ 2%, and ferment 12 ~ 24h under agitation;
(6) ultrasonic assistant secondary lixiviate: after fermentation ends under ultrasonic assistant effect secondary lixiviate 10 ~ 20min, extraction temperature controls at 60 ~ 80 DEG C;
(7) centrifugal: centrifugal supernatant, filter residue adds 3 ~ 5 times of pure water, centrifugal, merges supernatant and is leaching liquor;
(8) prepare burden: add in 1 ~ 1.5g/L xylitol, 1 ~ 2g/L calcium lactate, 5 ~ 10g/L citric acid, 10 ~ 20g/L oligoisomaltose, 50mg/L calgon, 50mg/L Vc, 50mg/L potassium sorbate ratio in leaching liquor and prepare burden and stir;
(9) micro-pore-film filtration: namely obtain gynostemma pentaphylla Chinese herbal medicine health care cold tea finished product with the micro-pore-film filtration leaching liquor that aperture is 1.5 μm;
(10) sterile filling sealing: filling and sealing in sterile filling production line.
CN201510393057.7A 2015-07-07 2015-07-07 Complex fermented gynostemma pentaphylla type Chinese herbal medicine health tea Pending CN104904962A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
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CN106387237A (en) * 2016-12-10 2017-02-15 青岛嘉瑞生物技术有限公司 Fermentation type Chinese-herbal-medicine health-care herbal tea with herba of salicornia europaea
CN107227226A (en) * 2017-07-07 2017-10-03 平利县绞股蓝研究所 A kind of compound gynostemma pentaphylla powder sweet rice wine and preparation method thereof
CN108478707A (en) * 2018-04-18 2018-09-04 罗仕伟 A kind of fermented type herbal health care preparation and preparation method thereof
CN108771727A (en) * 2018-08-09 2018-11-09 黔西南州长霖种植农民专业合作社 A kind of anti-inflammatory radix tetrastigme health protection tea of heat-clearing and preparation method thereof
CN110326692A (en) * 2019-07-29 2019-10-15 时秀林 A kind of birch young pilose antler health-care cold tea
CN110823819A (en) * 2019-08-19 2020-02-21 广西中医药大学 Compound radix polygoni multiflori preparata mulberry parasitic tea, preparation method and quality detection method

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CN103168889A (en) * 2013-04-08 2013-06-26 贵州汇强科技有限公司 Fermented kudzuvine root herbal tea and preparation method thereof
CN103583759A (en) * 2013-11-26 2014-02-19 青岛嘉瑞生物技术有限公司 Health-care herbal tea prepared from Chinese herbal medicines including gynostemma pentaphylla
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105994843A (en) * 2016-06-27 2016-10-12 青岛铭晨信息技术有限公司 Suaeda glaucu bunge herb healthcare herbal tea
CN106387237A (en) * 2016-12-10 2017-02-15 青岛嘉瑞生物技术有限公司 Fermentation type Chinese-herbal-medicine health-care herbal tea with herba of salicornia europaea
CN107227226A (en) * 2017-07-07 2017-10-03 平利县绞股蓝研究所 A kind of compound gynostemma pentaphylla powder sweet rice wine and preparation method thereof
CN108478707A (en) * 2018-04-18 2018-09-04 罗仕伟 A kind of fermented type herbal health care preparation and preparation method thereof
CN108771727A (en) * 2018-08-09 2018-11-09 黔西南州长霖种植农民专业合作社 A kind of anti-inflammatory radix tetrastigme health protection tea of heat-clearing and preparation method thereof
CN110326692A (en) * 2019-07-29 2019-10-15 时秀林 A kind of birch young pilose antler health-care cold tea
CN110823819A (en) * 2019-08-19 2020-02-21 广西中医药大学 Compound radix polygoni multiflori preparata mulberry parasitic tea, preparation method and quality detection method

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Application publication date: 20150916