CN104788535B - The method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously - Google Patents

Abstract

The invention discloses a kind of method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, including：Step 1: slurries obtain；Step 2: adding polysaccharide composite enzyme enzymolysis, a shinyleaf yellowhorn oil is obtained；Step 3: being once demulsified, secondary shinyleaf yellowhorn oil is obtained；Step 4: secondary demulsification, obtains shinyleaf yellowhorn oil three times, and mixed with a shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil, obtain shinyleaf yellowhorn oil；A Step 5: alkali carries；Step 6: secondary alkali carries；Step 7: acid is heavy, shiny-leaved yellowhorn albumen is obtained.The method that aqueous enzymatic method provided by the invention prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, technique is simple and safe, and the shinyleaf yellowhorn oil fragrance of extraction is denseer, of light color, and quality is good；Obtained shiny-leaved yellowhorn purity of protein is higher, and protein content is higher than 50%, can be widely used in as a kind of good functional ingredient in food processing.

Description

Aqueous enzymatic method for simultaneously preparing sorbifolia oil and radix sorbifolia protein

technical field

The invention relates to the technical field of intensive processing of agricultural products and comprehensive utilization of by-products thereof, in particular to a method for simultaneously preparing sorbifolia oil and radix sorbifolium protein by an aqueous enzymatic method.

Background technique

Xantho sorbifolium has high industrial value and nutritional value, and the oil content in the seeds and kernels is extremely high. The research results prove that the oil content in the kernels reaches 55-67%, and the oil content in the kernels of fine varieties reaches 72%. %, more than ordinary oil plants, the basic composition of its oil is as follows: stearic acid, oleic acid 38.9% (one of the main components of general edible oil), linoleic acid 40.2% (similar to soybean oil and walnut oil, but also the nutritional value The highest part), behenic acid 7.2%, linolenic acid and glycenoic acid are 0.3% each. Xingguan fruit is a unique economic woody oil tree species in my country. The oil content of the seeds is 30.4% to 47%, and the oil content of the kernels is as high as 66.39%. The oil is yellow and transparent, and it is delicious to eat. The main ingredient of Ning Ning has an excellent effect on lowering blood pressure. Eating Xanthan fruit oil can effectively prevent hypertension, hyperlipidemia, arteriosclerosis and other diseases.

The emulsifying property of Xingguan fruit protein is good, and Xingrong fruit protein can be used as an emulsifier, which can be added to baked food, frozen food and soup food to improve the stability of the product. The amino acid content of sorbifolia protein is rich, reaching 21.68%, and sorbifolium protein is a protein with relatively good quality. According to the recommendation of WHO, taking the ratio of amino acids contained in egg protein as the reference standard, the amino acid evaluation score of sorbifolia protein is better than that of peanut protein in the absence of tryptophan. Therefore, it is necessary to study a method for simultaneously preparing sorbifolia oil and sorbifolia protein.

Contents of the invention

Aiming at the deficiencies in the above-mentioned technologies, the present invention provides a method for simultaneously preparing sorbifolia oil and radix sorbifolium protein by an aqueous enzymatic method. Crown fruit protein is more pure.

The technical scheme adopted by the present invention to solve the technical problem is: a method for simultaneously preparing sorbifolia oil and sorbifolia protein by an aqueous enzymatic method, comprising the following steps: Step 1, slurry acquisition: mixing sorbifolia kernel raw materials with water homogeneously to obtain a slurry; step 2, enzymatic hydrolysis: after adjusting the pH of the slurry, add a polysaccharide compound enzyme for enzymatic hydrolysis, and after the enzymatic hydrolysis reaction is completed, the enzymatic hydrolysis solution is centrifuged to obtain the first layer of clear oil, the second layer of emulsified layer, and the second layer of The third layer of waste liquid and the fourth layer of crude protein are absorbed layer by layer to obtain the first layer of sorbifolia oil, the first emulsification layer and the first crude protein solution; step 3, the first demulsification: separate the first emulsification layer, freeze and then thaw to break the demulsification, and obtain The primary emulsion is centrifuged to obtain the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid in sequence, and the second layer of Xanthania sorbifolium oil and the second emulsified layer are obtained by absorbing layer by layer; step 4, the second emulsification layer: Separate the secondary emulsified layer, freeze and then thaw and demulsify to obtain the secondary emulsion and centrifuge it to obtain the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid in sequence, and absorb the upper layer of clear oil to obtain the third layer of xanthan fruit oil , and mixed with the first X. sorbifolium oil and the second X. X. sorbifolia oil to obtain X. sorbifolia oil; Step 5, an alkali extraction: carry out an alkali extraction on a crude protein solution to obtain an alkali extraction solution and extract it Centrifuge to obtain the upper protein solution and the lower layer of precipitation, which are separated to obtain the primary protein solution and the primary precipitation; step 6, secondary alkali extraction: the primary precipitation is subjected to secondary alkaline extraction to obtain the secondary alkaline extraction solution and centrifuged to obtain The upper protein solution and the lower layer are precipitated, and are separated to obtain the secondary protein solution; Step 7, acid precipitation: mix the primary protein solution and the secondary protein solution, adjust the pH, and carry out acid precipitation centrifugation to obtain the precipitated powder, and carry out the precipitation on the precipitated powder Freeze-drying to obtain xanthocarpus protein.

Preferably, the preparation process of the raw material of X. sorbifolia nuts in said step 1 is as follows: shelling and peeling the X. sorbifolia nuts to obtain pure X. sorbifolia nuts, crushing and sieving the pure X. sorbifolia nuts to obtain particles with a particle size of 40-80 mesh Raw material of sorbifolia nuts.

Preferably, the slurry in the step 1 is prepared by uniformly mixing the raw material of sorbifolia nuts and water according to the ratio of material to liquid: 1g: 4-6mL.

Preferably, in the second step, hydrochloric acid or citric acid is added to the slurry to adjust the pH of the slurry to 4-6, and a polysaccharide complex enzyme is added for enzymolysis. The enzymolysis reaction temperature is 45-55°C, and the enzymolysis reaction time is 2-6 After the enzymatic hydrolysis reaction is completed, the enzymatic hydrolysis solution is heated to 75-85°C to inactivate the enzyme for 15-25 minutes, the pH of the enzymatic hydrolysis solution is adjusted to 4-5, and then centrifuged.

Preferably, the polysaccharide compound enzyme in the step 2 uses cellulase + pectinase, the dosage of the cellulase and pectinase is 1:0.5-10, and the enzyme amount of the polysaccharide compound enzyme is The amount of raw material for nuts is 500-2000u/g.

Preferably, the primary demulsification and secondary demulsification conditions are: freezing temperature -25°C, freezing time 24 hours, thawing temperature 40°C, thawing 4 hours.

Preferably, the conditions for one alkaline extraction in step five are: the ratio of alkaline extraction to liquid is 1:5-10, the alkaline extraction temperature is 50-60°C, the alkaline extraction pH is 8-10, and the alkaline extraction time is 1-3 hours.

Preferably, the conditions for the second alkaline extraction in step 6 are: the ratio of alkaline extraction to liquid is 1:10-15, the alkaline extraction temperature is 50-60°C, the alkaline extraction pH is 8-10, and the alkaline extraction time is 0.5-2 hours.

Preferably, in the step seven, the primary protein solution and the secondary protein solution are mixed, and hydrochloric acid or citric acid is added to adjust the pH to 4.6 for acid precipitation. The acid precipitation temperature is 40°C, and centrifuged after flocs appear.

The present invention compares with prior art, and its beneficial effect is:

(1) The present invention uses polysaccharide complex enzyme enzymolysis to destroy the cell structure to release oil, which has a higher yield than cellulase or pectinase enzymolysis alone; compared with protease enzymolysis, polysaccharide compound The optimal pH of the cellulase and pectinase in the enzyme is under acidic conditions, so only one enzymatic hydrolysis is required, and when protease is used for enzymatic hydrolysis, due to the difference in reaction conditions, the protease is mostly alkaline or neutral , so two enzymatic hydrolysis is required, increasing the enzymatic hydrolysis reaction time by about 2 to 5 hours; in addition, when protease is used for enzymatic hydrolysis, it is easy to hydrolyze the protein into polypeptides, which reduces the yield of protein, and the purity of the obtained protein product is low , and when using polysaccharide complex enzyme enzymolysis, and the crude protein solution obtained by demulsification alkali extraction and acid precipitation method, the protein yield can be increased to 40% and the purity is increased, which greatly increases the protein yield.

(2) Since the aqueous enzymatic method will form an emulsion, its components are oil, water, a small amount of protein, starch and cellulose, compared to heating demulsification, microwave irradiation and salting out demulsification, the present invention adopts Two consecutive freezing and thawing demulsification can better destroy the stability of the emulsion, because the oil phase crystallization will appear in the emulsion during the freezing process, and these fat crystals can penetrate into the water phase, when these fat crystals happen to appear in the adjacent oil When between drops, the interfacial membrane will be pierced to cause the aggregation of oil droplets, thereby greatly reducing the stability of the emulsion and achieving the purpose of demulsification. The present invention greatly improves the yield of oil by adopting two consecutive freezing and thawing demulsification methods. The oil yield has been increased from about 20% before demulsification to the highest 40%, fully extracting the oil in Xingguan fruit.

(3) The present invention extracts sorbifolia protein by using two consecutive alkali extraction and acid precipitation methods, and changes the conditions of the two alkaline extractions before and after, and increases the solid-liquid ratio of the second alkaline extraction, which can effectively promote the extraction of sorbifolia protein. Dissolution and suction; due to the increase of the liquid ratio of the secondary alkali extraction, the time is correspondingly reduced, and in the later stage of the second alkali extraction, the dissolution of protein decreases with the increase of time, so by changing the time of the two alkaline extractions before and after, the equipment can be made It has been fully utilized and the production efficiency has been improved.

(4) The water enzymatic method provided by the present invention simultaneously prepares sorbifolia oil and sorbifolia protein. The process is simple and safe, and the extracted sorbifolia oil has a strong fragrance, light color and good quality; Higher, the protein content is higher than 50%, and can be widely used in food processing as a good functional ingredient.

Description of drawings

Fig. 1 is the process flow diagram of the method for simultaneously preparing sorbifolia oil and radix sorbifolium protein by the aqueous enzymatic method of the present invention.

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

As shown in Fig. 1, the present invention provides a kind of method that hydroenzymatic method prepares sorbifolia oil and radix sorbifolium protein simultaneously, comprises the steps:

Step 1. Slurry acquisition: Shelling and peeling the radix sorbifolia nuts to obtain pure radix sorbifolia nuts, crushing and sieving the pure radix sorbifolia nuts to obtain the raw material of radix sorbifolia nuts with a particle size of 40 to 80 meshes, and reducing the radishes sorbifolia kernels The particle size of the raw material increases the specific surface area of the molecule, which can increase the contact area of the enzymatic hydrolysis, thereby better promoting the reaction and benefiting oil production. Mix the raw material of Xantho sorbifolia nuts and water according to the ratio of material to liquid: 1g: 4 ~ 6mL, The slurry is prepared, and the ratio of the concentration of the enzyme to the substrate can be guaranteed to be suitable within the range of the material-to-liquid ratio;

Step 2, enzymatic hydrolysis: add hydrochloric acid or citric acid to the slurry to adjust the pH of the slurry to 4-6, add polysaccharide compound enzyme for enzymolysis, the polysaccharide compound enzyme uses cellulase + pectinase, cellulase and pectinase The optimum pH is between 4-6, the optimum temperature is 45-55°C, the dosage of cellulase and pectinase is 1:0.5-10, and the enzyme dosage of polysaccharide compound enzyme is The amount of nut raw materials used is 500-2000u/g, the enzymolysis reaction temperature is 45-55°C, and the enzymolysis reaction time is 2-6 hours. After the enzymolysis reaction is completed, the enzymolysis solution is heated to 75-85°C to kill the enzyme 15 ~25 minutes, adjust the pH of the enzymolysis solution to 4~5 and then centrifuge. The centrifugation conditions are: speed 4000~5500r/min, centrifuge for 10~30min, and then get the first layer of clear oil, the second layer of emulsified layer, and the third layer of waste liquid And the fourth layer of crude protein, absorbing layer by layer to obtain the first layer of xanthan fruit oil, the first emulsification layer and the first crude protein solution;

Step 3, primary demulsification: separate the primary emulsified layer, freeze and then thaw and demulsify. The primary demulsification conditions are: freezing temperature -25°C, freezing time 24 hours, thawing temperature 40°C, thawing for 4 hours, to obtain a primary emulsion and It is centrifuged, and the centrifugal conditions are as follows: rotating speed 4000-5500r/min, centrifuging for 10-30min, successively obtain the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid, and absorb layer by layer to obtain the second layer of Xanthania sorbifolium oil and the second layer of oil. sub-emulsion layer;

Step 4, secondary demulsification: separate the secondary emulsified layer, freeze and then thaw and demulsify. The secondary demulsification conditions are: freezing temperature -25°C, freezing time 24 hours, thawing temperature 40°C, thawing 4 hours, Obtain the secondary emulsion and centrifuge it. The centrifugation conditions are as follows: rotating speed 4000-5500r/min, centrifuging for 10-30min, successively obtain the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid, absorb the upper layer of clear oil to obtain the third layer of liquid Xanthan fruit oil, and mixed with the primary xanthus xanthus oil and the secondary xanthus xanthus oil, to obtain the xanthus xanthus oil;

Step 5. Alkaline extraction once: carry out an alkali extraction on a crude protein solution, and the protein can be fully dissolved in an alkaline environment. The conditions for an alkali extraction are: the ratio of alkali extraction material to liquid is 1:5~10, and the alkali extraction temperature is 50~ 60°C, alkaline extraction pH 8-10, alkaline extraction time 1-3 hours, obtain an alkaline extraction solution and centrifuge it, the centrifugation conditions are: speed 4000-5500r/min, centrifuge 10-30min, obtain the upper layer protein solution and the lower layer precipitate , which is separated to obtain a protein solution and a precipitation;

Step 6. Second alkali extraction: carry out the second alkali extraction on the first precipitation. The purpose of the second alkali extraction is to ensure that the undissolved protein can be fully dissolved during the first alkali extraction. The condition of the second alkali extraction is: the ratio of alkali extraction to liquid is 1 : 10～15, alkali extraction temperature 50-60 ℃, alkali extraction pH8-10, alkali extraction time 0.5～2 hours, get the secondary alkali extraction solution and centrifuge it, centrifugation conditions are: rotating speed 4000～5500r/min, centrifugation After 10-30 minutes, the upper layer protein solution and the lower layer precipitate are obtained, which are separated to obtain the secondary protein solution. The increase of the solid-liquid ratio during the secondary alkaline extraction is to fully dissolve the protein and increase the protein yield;

Step 7. Acid precipitation: mix the primary protein solution and the secondary protein solution, add hydrochloric acid or citric acid to adjust the pH to 4.6 for acid precipitation (the isoelectric point of X. Centrifuge after the flocs appear, and the centrifugation conditions are as follows: rotating speed 4000-5500r/min, centrifuging for 10-30min to obtain precipitated powder, which is freeze-dried to obtain sorbifolia protein.

Compared with the prior art, the present invention has the beneficial effects that: the present invention adopts polysaccharide complex enzyme enzymolysis to destroy the cell structure to release the oil, and the yield of oil is higher than that of cellulase or pectinase enzymolysis alone. High; compared with enzymatic hydrolysis with protease, the optimum pH of cellulase and pectinase in polysaccharide complex enzymes falls under acidic conditions, so only one enzymatic hydrolysis is required, and when enzymatic hydrolysis with protease, due to the reaction The conditions are different, proteases are mostly alkaline or neutral, so two enzymatic hydrolysis is required, and the enzymatic hydrolysis reaction time is increased by about 2 to 5 hours; in addition, when protease is used for enzymatic hydrolysis, it is easy to hydrolyze the protein into polypeptides, reducing the protein Yield, and the purity of the obtained protein product is low, and when the crude protein solution obtained by enzymatic hydrolysis with polysaccharide compound enzyme and the method of demulsification, alkaline extraction and acid precipitation can increase the protein yield to 40% and increase the purity, Greatly increased protein yield. Since an emulsion will be formed when the aqueous enzymatic method is used to extract oil, its components are oil, water, a small amount of protein, starch and cellulose. Compared with heating demulsification, microwave irradiation and salting out demulsification, the present invention uses two Freezing and thawing demulsification can better destroy the stability of the emulsion, because the oil phase crystallization will appear in the emulsion during the freezing process, and these fat crystals can penetrate into the water phase, when these fat crystals happen to appear between adjacent oil droplets When the interface film is pierced, the oil droplets will gather, thereby greatly reducing the stability of the emulsion and achieving the purpose of demulsification. The present invention greatly improves the oil yield and oil yield by adopting two consecutive freeze-thaw demulsification methods. From about 20% before demulsification to the highest 40%, the oil in Xingguan fruit is fully extracted. The present invention extracts radix sorbifolium protein by adopting two consecutive alkali extraction and acid precipitation methods, changes the conditions of the two alkaline extractions before and after, and increases the solid-liquid ratio of the second alkaline extraction, which can effectively promote the dissolution and absorption of sorbiflora protein ;Due to the increase of the liquid ratio of the secondary alkali extraction, the time is correspondingly reduced, and in the later stage of the secondary alkali extraction, the dissolution of protein decreases with the increase of time, so the equipment can be fully utilized by changing the time of the two alkaline extractions before and after. , improving production efficiency. The method for simultaneously preparing sorbifolia oil and radix sorbifolium protein by the aqueous enzymatic method provided by the present invention has a simple and safe process, and the extracted radix sorbifolium oil has strong fragrance, light color and good quality; the obtained radix sorbifolia protein has higher purity and The protein content is higher than 50%, which can be widely used in food processing as a good functional ingredient.

Example 1:

Shell and peel the radix sorbifolia nuts to obtain pure radix sorbifolia nuts, crush and sieve the pure radix sorbifolia nuts to obtain the raw material of radix sorbifolia nuts with a particle size of 60 mesh, mix the raw materials of radix sorbifolia nuts with water according to the ratio of material to liquid: 1g: Mix 5 mL to make a slurry; add hydrochloric acid to the slurry to adjust the pH of the slurry to 5, add polysaccharide compound enzyme for enzymatic hydrolysis, the polysaccharide compound enzyme uses cellulase + pectinase, the dosage of the cellulase and pectinase The ratio is 1:1, and the amount of polysaccharide compound enzyme added is 1000u/g of the amount of raw material of X. Heat the solution to 80°C to inactivate the enzyme for 20 minutes, adjust the pH of the enzymolysis solution to 4.6, and then centrifuge at a speed of 5500r/min for 10 minutes to obtain the first layer of clear oil, the second layer of emulsified layer, and the third layer of waste liquid And the fourth layer of crude protein, absorb layer by layer to obtain the first layer of xanthan fruit oil, the first emulsification layer and the first crude protein liquid; separate the first emulsification layer, freeze and then thaw to break the emulsification, the first emulsification condition is: freezing temperature -25 ℃, Freeze for 24 hours, thaw at 40°C, thaw for 4 hours, obtain an emulsion and centrifuge it. The centrifugation conditions are: speed 4000/min, centrifuge for 10 minutes, and then obtain the first layer of clear oil, the second layer of emulsified layer and the third layer The waste liquid is absorbed layer by layer to obtain the secondary xanthania sorbifolia oil and the secondary emulsification layer; the secondary emulsification layer is separated, frozen and then thawed to break the emulsion. The conditions of the second emulsion break are: freezing temperature -25 ° C, freezing time 24 Hours, thawing temperature 40 ℃, thawing 4 hours, get the secondary emulsion and centrifuge it, the centrifugation conditions are: rotating speed 4000r/min, centrifugation 10min, get the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid in sequence , absorbing the supernatant clear oil to obtain three times of Xantho sorbifolia oil, and mixing with the first and second times of X. sorbifolia oil to obtain X. It is: the ratio of alkali extraction to liquid is 1:5, the temperature of alkali extraction is 60°C, the pH of alkali extraction is 9, the time of alkali extraction is 3 hours, and the alkali extraction solution is obtained once and centrifuged. The upper layer of protein solution and the lower layer of precipitation are separated to obtain a primary protein solution and a primary precipitation; the primary precipitation is subjected to secondary alkali extraction, and the conditions of the secondary alkaline extraction are: the ratio of alkaline extraction to liquid is 1:10, and the temperature of alkaline extraction is 60°C. Alkaline extraction pH9, alkaline extraction time 1 hour, obtain the secondary alkaline extraction solution and centrifuge it, the centrifugation conditions are: rotating speed 4000r/min, centrifugation 10min, obtain the upper layer protein solution and the lower layer precipitate, separate them to obtain the secondary protein solution; Mix the primary protein solution and the secondary protein solution, add hydrochloric acid or citric acid to adjust the pH to 4.6 for acid precipitation, the acid precipitation temperature is 40°C, and centrifuge after flocs appear. After 10 minutes, the precipitated powder was obtained, and the precipitated powder was freeze-dried to obtain X. sorbifolia protein; the yield of X. sorbifolia oil was 35.6%, the extraction rate was 61.4%, the purity (dry basis) of X. sorbifolia protein was 51.6%, and the extraction rate was 40.8%. Among them, the oil content of sorbifolia nuts is 58.2%, and the protein content is 29.7%.

Example 2:

Shell and peel the radix sorbifolia nuts to obtain pure radix sorbifolia nuts, crush and sieve the pure radix sorbifolia nuts to obtain the raw material of radix sorbifolia nuts with a particle size of 60 mesh, mix the raw materials of radix sorbifolia nuts with water according to the ratio of material to liquid: 1g: Mix 5 mL to make a slurry; add hydrochloric acid to the slurry to adjust the pH of the slurry to 5, add polysaccharide compound enzyme for enzymatic hydrolysis, the polysaccharide compound enzyme uses cellulase + pectinase, the dosage of the cellulase and pectinase The ratio is 1:10, and the amount of polysaccharide compound enzyme added is 2000u/g of the raw material of Xanthos sorbifolium nuts, the enzymolysis reaction temperature is 50°C, and the enzymolysis reaction time is 5 hours. After the enzymolysis reaction is completed, the enzymolysis reaction Heat the solution to 80°C to inactivate the enzyme for 20 minutes, adjust the pH of the enzymolysis solution to 4.6, and then centrifuge at a speed of 5500r/min for 10 minutes to obtain the first layer of clear oil, the second layer of emulsified layer, and the third layer of waste liquid And the fourth layer of crude protein, absorb layer by layer to obtain the first layer of xanthan fruit oil, the first emulsification layer and the first crude protein liquid; separate the first emulsification layer, freeze and then thaw to break the emulsification, the first emulsification condition is: freezing temperature -25 ℃, Freeze for 24 hours, thaw at 40°C, thaw for 4 hours, obtain an emulsion and centrifuge it. The centrifugation conditions are: speed 4000/min, centrifuge for 10 minutes, and then obtain the first layer of clear oil, the second layer of emulsified layer and the third layer The waste liquid is absorbed layer by layer to obtain the secondary xanthania sorbifolia oil and the secondary emulsification layer; the secondary emulsification layer is separated, frozen and then thawed to break the emulsion. The conditions of the second emulsion break are: freezing temperature -25 ° C, freezing time 24 Hours, thawing temperature 40 ℃, thawing 4 hours, get the secondary emulsion and centrifuge it, the centrifugation conditions are: rotating speed 4000r/min, centrifugation 10min, get the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid in sequence , absorbing the supernatant clear oil to obtain three times of Xantho sorbifolia oil, and mixing with the first and second times of X. sorbifolia oil to obtain X. It is: the ratio of alkali extraction to liquid is 1:5, the temperature of alkali extraction is 60°C, the pH of alkali extraction is 9, the time of alkali extraction is 3 hours, and the alkali extraction solution is obtained once and centrifuged. The upper layer of protein solution and the lower layer of precipitation are separated to obtain a primary protein solution and a primary precipitation; the primary precipitation is subjected to secondary alkali extraction, and the conditions of the secondary alkaline extraction are: the ratio of alkaline extraction to liquid is 1:15, and the temperature of alkaline extraction is 60°C. Alkaline extraction pH9, alkaline extraction time 2 hours, get the secondary alkaline extraction solution and centrifuge it, the centrifugation conditions are: rotating speed 4000r/min, centrifugation 10min, get the upper layer protein solution and the lower layer precipitate, separate them to get the secondary protein solution; Mix the primary protein solution and the secondary protein solution, add hydrochloric acid or citric acid to adjust the pH to 4.6 for acid precipitation, the acid precipitation temperature is 40°C, and centrifuge after flocs appear. After 10 minutes, the precipitated powder was obtained, and the precipitated powder was freeze-dried to obtain X. sorbifolia protein; the yield of X. sorbifolia oil was 41.6%, the extraction rate was 75.4%, the purity (dry basis) of X. sorbifolia protein was 55.9%, and the extraction rate was 48.1%. Among them, the oil content of sorbifolia nuts is 55.1%, and the protein content is 29.9%.

Example 3:

Shell and peel the radix sorbifolia nuts to obtain pure radix sorbifolia nuts, crush and sieve the pure radix sorbifolia nuts to obtain the raw material of radix sorbifolia nuts with a particle size of 60 mesh, mix the raw materials of radix sorbifolia nuts with water according to the ratio of material to liquid: 1g: Mix 5 mL to make a slurry; add hydrochloric acid to the slurry to adjust the pH of the slurry to 5, add polysaccharide compound enzyme for enzymatic hydrolysis, the polysaccharide compound enzyme uses cellulase + pectinase, the dosage of the cellulase and pectinase The ratio is 1:2, and the amount of polysaccharide compound enzyme added is 1000u/g of the amount of raw material of X. Heat the solution to 80°C to inactivate the enzyme for 20 minutes, adjust the pH of the enzymolysis solution to 4.6, and then centrifuge at a speed of 5000r/min for 10 minutes to obtain the first layer of clear oil, the second layer of emulsified layer, and the third layer of waste liquid And the fourth layer of crude protein, absorb layer by layer to obtain the first layer of xanthan fruit oil, the first emulsification layer and the first crude protein liquid; separate the first emulsification layer, freeze and then thaw to break the emulsification, the first emulsification condition is: freezing temperature -25 ℃, Freeze for 24 hours, thaw at 40°C, and thaw for 4 hours to obtain an emulsion and centrifuge it at a speed of 5000/min for 10 minutes to obtain the first layer of clear oil, the second layer of emulsified layer and the third layer The waste liquid is absorbed layer by layer to obtain the secondary xanthania sorbifolia oil and the secondary emulsification layer; the secondary emulsification layer is separated, frozen and then thawed to break the emulsion. The conditions of the second emulsion break are: freezing temperature -25 ° C, freezing time 24 Hours, thawing temperature 40 ℃, thawing 4 hours, get the secondary emulsion and centrifuge it, the centrifugation conditions are: rotating speed 5000r/min, centrifugation 10min, get the first layer of clear oil, the second layer of emulsified layer and the third layer of waste liquid in sequence , absorbing the supernatant clear oil to obtain three times of Xantho sorbifolia oil, and mixing with the first and second times of X. sorbifolia oil to obtain X. It is: the ratio of alkali extraction to liquid is 1:5, the temperature of alkali extraction is 60°C, the pH of alkali extraction is 8.5, and the time of alkali extraction is 2 hours. The alkali extraction solution is obtained once and centrifuged. The centrifugation conditions are: speed 4000r/min, centrifugation 10min , to obtain the upper layer protein solution and the lower layer of precipitation, which are separated to obtain the primary protein solution and the primary precipitation; the primary precipitation is subjected to secondary alkali extraction, and the conditions of the secondary alkaline extraction are: the ratio of alkali extraction material to liquid is 1:15, and the alkali extraction temperature is 60 ℃, alkaline extraction pH 8.5, alkaline extraction time 2 hours, obtain the secondary alkaline extraction solution and centrifuge it, the centrifugation conditions are: rotating speed 4000r/min, centrifugation 10min, obtain the upper layer protein solution and the lower layer precipitate, which are separated to obtain two Secondary protein solution; mix the primary protein solution and the secondary protein solution, add hydrochloric acid or citric acid to adjust the pH to 4.6 for acid precipitation, the acid precipitation temperature is 40°C, centrifuge after flocs appear, the centrifugation condition is: speed 4000r /min, centrifuged for 10min, to obtain the precipitated powder, freeze-dried the precipitated powder, to obtain the Xanthania sorbifolium protein; the yield of Xantho sorbifolia oil was 37.0%, the extraction rate was 65.9%, and the purity of the Xantho sorbifolia protein (dry basis) was 56.2%. The rate is 44.7%. Among them, the oil content of sorbifolia nuts is 56.1%, and the protein content is 28.9%.

Claims (5)

1. a kind of method that aqueous enzymatic method prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, it is characterised in that comprise the following steps：

Step 1: slurries obtain：Shiny-leaved yellowhorn benevolence raw material and water are mixed, slurries are made；

Step 2: enzymolysis：Hydrochloric acid or citric acid are added in slurries slurries pH is adjusted to 4~6, add cellulase and pectase Enzymolysis, the dosage of the cellulase and pectase is 1：0.5~10, and the enzyme concentration of cellulase and pectase is shiny-leaved yellowhorn 500~2000u/g of benevolence raw material dosage, enzyme digestion reaction temperature are 45~55 DEG C, and the enzyme digestion reaction time is 2~6 hours, waits to digest Reaction terminates, and enzymolysis liquid is warming up into 75~85 DEG C of enzyme deactivations 15~25 minutes, centrifuged after adjusting enzymolysis liquid pH to 4~5, successively To first layer edible vegetable oil, second layer emulsion layer, third layer waste liquid and the 4th layer of crude protein, successively draw obtain a shinyleaf yellowhorn oil, Emulsion layer and a crude protein liquid；

Step 3: once it is demulsified：Emulsion layer is separated, demulsification of thawing again is freezed, obtains an emulsion and centrifuged, according to It is secondary to obtain first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid, successively draw and obtain secondary shinyleaf yellowhorn oil and second emulsifying Layer；

Step 4: secondary demulsification：Second emulsifying layer is separated, demulsification of thawing again is freezed, obtains secondary emulsion and centrifuged, according to It is secondary to obtain first layer edible vegetable oil, second layer emulsion layer and third layer waste liquid, draw upper strata edible vegetable oil and obtain shinyleaf yellowhorn oil three times, and with one Secondary shinyleaf yellowhorn oil and secondary shinyleaf yellowhorn oil mix, and shinyleaf yellowhorn oil is made；

A Step 5: alkali carries：Crude protein liquid is subjected to an alkali carries, the condition of an alkali carries is：Alkali carries solid-liquid ratio 1:5 ~10,50~60 DEG C of alkali carries temperature, alkali carries pH8~10,1~3 hour alkali carries time, obtain an alkali extract and centrifuged, Upper strata protein solution and lower sediment are obtained, is isolated to obtain a protein liquid and primary sedimentation；

Step 6: secondary alkali carries：Primary sedimentation is subjected to secondary alkali carries, the condition of secondary alkali carries is：Alkali carries solid-liquid ratio 1:10~ 15,50-60 DEG C of alkali carries temperature, alkali carries pH8-10,0.5~2 hour alkali carries time, obtain secondary alkali extract and centrifuged, obtained To upper strata protein solution and lower sediment, it is isolated to obtain secondary protein liquid；

Step 7: acid is heavy：Protein liquid and secondary protein liquid are mixed, the heavy centrifugation of acid is carried out after adjusting pH, obtains precipitated powder, Precipitated powder is freeze-dried, obtains shiny-leaved yellowhorn albumen.

2. the method that aqueous enzymatic method as claimed in claim 1 prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, it is characterised in that institute The shiny-leaved yellowhorn benevolence stock preparation process stated in step 1 is：Shiny-leaved yellowhorn decortication of shelling is obtained into pure shiny-leaved yellowhorn benevolence, by pure shiny-leaved yellowhorn Benevolence pulverizes and sieves, and obtains the shiny-leaved yellowhorn benevolence raw material that granular size is 40~80 mesh.

3. the method that aqueous enzymatic method as claimed in claim 2 prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, it is characterised in that institute State slurries in step 1 by by shiny-leaved yellowhorn benevolence raw material and water by solid-liquid ratio 1g：4~6mL, which is mixed, to be made.

4. the method that aqueous enzymatic method as claimed in claim 1 prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, it is characterised in that institute Stating once demulsification and secondary demulsification condition is：- 25 DEG C of cryogenic temperature, cooling time 24 hours, 40 DEG C of thaw point, defrosting 4 are small When.

5. the method that aqueous enzymatic method as claimed in claim 1 prepares shinyleaf yellowhorn oil and shiny-leaved yellowhorn albumen simultaneously, it is characterised in that institute State in step 7 and mix a protein liquid and secondary protein liquid, add hydrochloric acid or lemon acid for adjusting pH and sour heavy, acid is carried out to 4.6 Heavy temperature is 40 DEG C, is centrifuged after floccule to appear.