CN104663865A

CN104663865A - Method for extracting biological preservative from coptis chinensis and application of method

Info

Publication number: CN104663865A
Application number: CN201310624126.1A
Authority: CN
Inventors: 崔桂林
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-11-26
Filing date: 2013-11-26
Publication date: 2015-06-03

Abstract

The invention relates to a method for extracting a biological preservative from coptis chinensis and an application of the method. The method comprises the following steps: 1, selecting coptis chinensis, and removing impurities such as soil and tree leaves; 2, crushing coptis chinensis; 3, putting the crushed coptis chinensis into a round bottom flask, adding an ethanol solution in a material liquid ratio of 1:(5-7), putting the mixture in a reflux condensing tube, performing heating reflux for 0.5-1 hour in a hot water bath, cooling, standing for 0.5-1 hour, and performing suction filtration so as to obtain a filtrate; 4, adding the filtrate into the ethanol solution in a material liquid ratio of 1:(7-10), performing condensation reflux for 1-2 hours, cooling, standing for 0.5-1 hour, performing suction filtration and taking a filtrate; 5, combining the filtrates obtained in two steps, and depressurizing in a water pump so as to steam out the ethanol solution; and 6, adding 1-2% of an acetic acid solution into the extract in a volume ratio of 1:(1-2), heating to dissolve the components, performing suction filtration in hot so as to remove undissolved substances, thereby obtaining the biological preservative. As flowers and/or leaves of honeysuckle are adopted to extract the biological preservative for preserve dates, the preservative is safe, non-toxic and free of pollution and can be used for replacing chemical bactericides and preservatives for preserving dates, and problems of environment pollution caused by chemical bactericides, food safety and the like can be avoided.

Description

Method and the application thereof of bio-preservative is extracted from the coptis

Technical field

The present invention relates to biological preservation field, from the capsule of weeping forsythia, extract method and the application thereof of bio-preservative specifically.

Background technology

The coptis is herbaceos perennial, belongs to Ranunculaceae Coptis.The coptis is also a kind of conventional Chinese medicine, just the most on the books in Shennong's Herbal, and because its rhizome is beaded, look yellow, so be referred to as " coptis ".For the dry rhizome of the ranunculaceae plant coptis, the triangle leaf coptis and Yun Lian, practise title " taste connects ", " refined company ", " cloud connects " respectively.There is heat-clearing and damp-drying drug, effect of purging intense heat and detonicating.Its taste entrance is extremely bitter, has common saying cloud " a dumb person taking a dose of bitter medicine, has hardship could not explain ", has namely said wherein flavour.

The coptis is also a kind of conventional Chinese medicine, just the most on the books in Shennong's Herbal, and because its rhizome is beaded, look yellow, so be referred to as " coptis ".For the dry rhizome of the ranunculaceae plant coptis, the triangle leaf coptis and Yun Lian, practise title " taste connects ", " refined company ", " cloud connects " respectively.There is heat-clearing and damp-drying drug, effect of purging intense heat and detonicating.Its taste entrance is extremely bitter, has common saying cloud " a dumb person taking a dose of bitter medicine, has hardship could not explain ", has namely said wherein flavour.

The coptis has broad-spectrum antibacterial action, all has certain effect to staphylococcus aureus, hemolytic streptococcus, pneumococcus, diplococcus meningitidis, shigella dysenteriae, bacillus anthracis etc.

Fruit, because its moisture is high, delicious flavour; rich in nutritive value, but the respiratory intensity of general berry fruits is large, and protective tissue is poor; preservation and freshness is more difficult, and preservation term and shelf life are all shorter, can bring great loss like this for orchard worker and consumer.And the preservation method of berries fruit conventional at present mainly adopts chemical bactericide, and a large amount of uses of chemical bactericide, pathogenic microorganism can be caused to produce drug resistance, and environmental pollution; In addition, because cleaning is more difficult, be difficult to thoroughly wash away chemical bactericide, after edible, health hidden danger can be caused.

Summary of the invention

According to above-mentioned weak point, the object of this invention is to provide a kind of method and application thereof of from the capsule of weeping forsythia, extracting bio-preservative.

For achieving the above object, technical scheme of the present invention is: a kind of method extracting bio-preservative from the coptis, and its operating procedure is as follows: 1, cleaned by the coptis, foreign material such as removing earth, leaf etc.; 2, the coptis is pulverized; 3, the coptis of flour is put into round-bottomed flask, be that the ratio of 1:5-7 adds ethanolic solution, load onto reflux condensing tube, add hot reflux 0.5-1h in hot bath with solid-liquid ratio, and cooling leaves standstill 0.5-1h, suction filtration, gets filtrate; 4, be that the ratio of 1:7-10 adds ethanolic solution, condensing reflux 1-2h again with solid-liquid ratio by filter residue, cooling leaves standstill 0.5-1h, and suction filtration, gets filtrate; 5, merge twice filtrate, under water pump decompression, steam ethanolic solution; 6, take volume ratio as the acetum that the ratio of 1:1-2 adds 1-2% in extract, heating for dissolving, suction filtration is to remove insoluble matter while hot, obtains bio-preservative.

Preferably: described coptis powder is broken to 100-200 order.

Preferably: filtrate is heated to 40-60 DEG C, fruit is soaked 2-5min in the solution, drop goes out air-dry, then preserves with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.

The present invention also provides a kind of application of extracting bio-preservative from the coptis, and described fruit storage condition is relative humidity 90-95%, and temperature is 2 ± 0.5 DEG C.

Preferably: described fruit is banana, orange, orange, mango, dragon fruit, mangosteen etc.

Beneficial effect of the present invention is: the present invention is that raw material extraction bio-preservative carries out fresh-keeping to fruit with the coptis, safety.Nontoxic, nuisanceless, fresh-keeping for fruit of chemical bactericide, anticorrisive agent can be replaced, avoid the problem such as pollution, food security of chemical bactericide to environment.

Detailed description of the invention

Below in conjunction with detailed description of the invention, the present invention will be further described.

Embodiment 1

From the coptis, extract a method for bio-preservative, its step is 1, is cleaned by the coptis, foreign material such as removing earth, leaf etc.; 2, the coptis is pulverized; 3, the coptis of flour is put into round-bottomed flask, be that the ratio of 1:5 adds ethanolic solution, load onto reflux condensing tube, add hot reflux 0.5h in hot bath with solid-liquid ratio, and cooling leaves standstill 0.5h, suction filtration, gets filtrate; 4, be that the ratio of 1:7 adds ethanolic solution, condensing reflux 1h again with solid-liquid ratio by filter residue, cooling leaves standstill 0.5h, and suction filtration, gets filtrate; 5, merge twice filtrate, under water pump decompression, steam ethanolic solution; 6, take volume ratio as the acetum that the ratio of 1:1 adds 1% in extract, heating for dissolving, suction filtration is to remove insoluble matter while hot, obtains bio-preservative.Wherein, coptis powder is broken to 100 orders.

The present invention also provide a kind of extract from the coptis bio-preservative application, filtrate is heated to 40 DEG C, fruit is soaked 2min in the solution, drop goes out air-dry, then preserves with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Described fruit storage condition is relative humidity 90%, and temperature is 1.5 DEG C.The bio-preservative extracted due to the coptis has certain bitter taste, the fruit that fruit is preferably eaten after the skin is removed, such as, be banana, orange, orange, mango, dragon fruit, mangosteen etc.

Embodiment 2

From the coptis, extract a method for bio-preservative, its step is 1, is cleaned by the coptis, foreign material such as removing earth, leaf etc.; 2, the coptis is pulverized; 3, the coptis of flour is put into round-bottomed flask, be that the ratio of 1:7 adds ethanolic solution, load onto reflux condensing tube, add hot reflux 1h in hot bath with solid-liquid ratio, and cooling leaves standstill 1h, suction filtration, gets filtrate; 4, be that the ratio of 1:10 adds ethanolic solution, condensing reflux 2h again with solid-liquid ratio by filter residue, cooling leaves standstill 1h, and suction filtration, gets filtrate; 5, merge twice filtrate, under water pump decompression, steam ethanolic solution; 6, take volume ratio as the acetum that the ratio of 1:2 adds 2% in extract, heating for dissolving, suction filtration is to remove insoluble matter while hot, obtains bio-preservative.Wherein, coptis powder is broken to 200 orders.

The present invention also provide a kind of extract from the coptis bio-preservative application, filtrate is heated to 60 DEG C, fruit is soaked 5min in the solution, drop goes out air-dry, then preserves with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Described fruit storage condition is relative humidity 95%, and temperature is 2.5 DEG C.The bio-preservative extracted due to the coptis has certain bitter taste, the fruit that fruit is preferably eaten after the skin is removed, such as, be banana, orange, orange, mango, dragon fruit, mangosteen etc.

Embodiment 3

From the coptis, extract a method for bio-preservative, its step is 1, is cleaned by the coptis, foreign material such as removing earth, leaf etc.; 2, the coptis is pulverized; 3, the coptis of flour is put into round-bottomed flask, be that the ratio of 1:6 adds ethanolic solution, load onto reflux condensing tube, add hot reflux 0.7h in hot bath with solid-liquid ratio, and cooling leaves standstill 0.8h, suction filtration, gets filtrate; 4, be that the ratio of 1:8 adds ethanolic solution, condensing reflux 1-21.5h again with solid-liquid ratio by filter residue, cooling leaves standstill 0.7h, and suction filtration, gets filtrate; 5, merge twice filtrate, under water pump decompression, steam ethanolic solution; 6, take volume ratio as the acetum that the ratio of 1:1-2 adds 1-2% in extract, heating for dissolving, suction filtration is to remove insoluble matter while hot, obtains bio-preservative.Wherein, coptis powder is broken to 100-200 order.

The present invention also provide a kind of extract from the coptis bio-preservative application, filtrate is heated to 50 DEG C, fruit is soaked 4min in the solution, drop goes out air-dry, then preserves with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Described fruit storage condition is relative humidity 85-95%, and temperature is 2 DEG C.The bio-preservative extracted due to the coptis has certain bitter taste, the fruit that fruit is preferably eaten after the skin is removed, such as, be banana, orange, orange, mango, dragon fruit, mangosteen etc.

Claims

1. from the coptis, extract a method for bio-preservative, it is characterized in that, its operating procedure is as follows:

(1) coptis is cleaned, foreign material such as removing earth, leaf etc.;

(2) coptis is pulverized;

(3) coptis of flour is put into round-bottomed flask, be that the ratio of 1:5-7 adds ethanolic solution, load onto reflux condensing tube, add hot reflux 0.5-1h in hot bath with solid-liquid ratio, and cooling leaves standstill 0.5-1h, suction filtration, gets filtrate;

(4) be that the ratio of 1:7-10 adds ethanolic solution, condensing reflux 1-2h again with solid-liquid ratio by filter residue, cooling leaves standstill 0.5-1h, and suction filtration, gets filtrate;

(5) merge twice filtrate, under water pump decompression, steam ethanolic solution;

(6) take volume ratio as the acetum that the ratio of 1:1-2 adds 1-2% in extract, heating for dissolving, suction filtration is to remove insoluble matter while hot, obtains bio-preservative.

2. according to the method extracting bio-preservative from the coptis according to claim 1, it is characterized in that, described coptis powder is broken to 100-200 order.

3. according to according to claim 1 extract from the coptis bio-preservative application, it is characterized in that, filtrate is heated to 40-60 DEG C, fruit is soaked 2-5min in the solution, drop goes out air-dry, then preserves with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.

4. according to application of extracting bio-preservative from the coptis according to claim 3, it is characterized in that: described fruit storage condition is relative humidity 90-95%, temperature is 2 ± 0.5 DEG C.

5. according to application of extracting bio-preservative from the coptis according to claim 4, it is characterized in that: described fruit is banana, orange, orange, mango, dragon fruit, mangosteen etc.