CN104604497A - Bletilla striata tissue culture seedling exercising and cultivation method - Google Patents
Bletilla striata tissue culture seedling exercising and cultivation method Download PDFInfo
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- CN104604497A CN104604497A CN201510043793.XA CN201510043793A CN104604497A CN 104604497 A CN104604497 A CN 104604497A CN 201510043793 A CN201510043793 A CN 201510043793A CN 104604497 A CN104604497 A CN 104604497A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention provides a bletilla striata tissue culture seedling exercising and cultivation method. The method comprises the steps that a matrix is prepared, seedling exercising is conducted in bottles, seedlings are taken out of the bottles, domestication and cultivation are conducted, and domestication management is conducted, wherein the domestication matrix is prepared by evenly mixing sandy soil or soil with sand, humus soil and sawdust. According to the bletilla striata tissue culture seedling transplantation method, every three cleaned tissue culture seedlings form a cluster, and the tissue culture seedlings are shallowly planted into a seedbed in a green onion arranging mode. By means of the cultivation method, the survival rate of the bletilla striata tissue culture seedlings transplanted to the field and the yield are increased, the transplanting survival rate of domesticated seedlings reaches over 95%, and the investment cost is lowered for a grower.
Description
Technical field
The invention belongs to traditional Chinese medicine planting technology field, be specifically related to a kind of hardening cultivation method of bletilla plantlet in vitro.
Background technology
Bletilla is that the orchid family bletilla belongs to herbaceos perennial, calls white root, Pseudobulbus Bletillae (Rhizoma Bletillae), Cold boiled chicken baby, YANGJIAOQI, purple orchid, triflorous ainsliaea leaf, company and grass etc.With stem tuber hyoscine, energy astringing to arrest bleeding, detumescence and promoting granulation; Control pulmonary tuberculosis hemoptysis, the haematemesis of bronchiectasis hemoptysis, stomach ulcer, hematuria, the disease such as to have blood in stool.The diseases such as traumatism and bleeding, burn and scald, rhagadia manus et pedis are controlled in external application.Huge commercial exploitation is had in biological medicine, health food, textile printing and dyeing, sapecial coating and daily-use chemical industry etc.Along with the demand of the exploitation of bletilla food and dyeing industry, beauty culture, bletilla consumption increases increasingly.
The seed nature germination rate of bletilla is extremely low, breeding difficulty, and wild resource is rare, and because demand is large, wild resource faces exhaustion frequently, now lists " endangered animal and plant kind international trade pact " in and is protected, therefore only take artificial breeding.Current conventional method is main mainly with plant division greatly, and to be cut into small pieces plantation by stem tuber, reproduction coefficient is low, cannot meet market demand.People start artificial propagation, and many places have employed tissue culture propagation method to obtain a large amount of seedling fast; Because tissue culture propagation carries out in artificial environment, its self-support capacity is very poor, particularly the absorption function of its coring, cane dredge transportation function, the photosynthesis function of blade can not gather and so adapt to extraneous environment, its due function can't be played completely, cause transplanting survival rate very low like this, therefore need to carry out domestication could transplant smoothly, to improve its survival rate.
Summary of the invention
For solving above-mentioned prior art Problems existing, the present invention proposes a kind of hardening cultivation method of bletilla plantlet in vitro, and this cultivation method improves bletilla plantlet in vitro to the survival rate in land for growing field crops and output, reduces the cost of investment at plantation family.
Technical scheme of the present invention is achieved in that
A hardening cultivation method for bletilla plantlet in vitro, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by sandy soil or folder sandy soil, humus soil and wood sawdust according to weight ratio 3:2:1 mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then cover the blade of plantlet in vitro with wet towel, and only root is exposed, finally root is placed in the carbendazim aqueous solution and soaks several minutes, transfer in the root-growing agent aqueous solution and soak several minutes;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, ditching, ridging makes seedbed; Then the plantlet in vitro step (3) cleaned transplants the seedbed to booth, and clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 4-7 month.
Further, described sandy soil or folder sandy soil, humus soil and wood sawdust by weight proportioning be (1 ~ 3): (1 ~ 2): (1 ~ 2), preferably 3:2:1.
Further, described step bletilla plantlet in vitro (2) to be cultivated spends the hardening phase at other booths, then only need put in this booth and just can transplant for 2 days.
Further, described step plantlet in vitro root (3) soaks 2 minutes with in 1000 times of carbendazim aqueous solution, transfers in 1000 times of root-growing agent aqueous solution to soak after 3 minutes to wait for transplanting.
Further, (4) described step is evenly sprayed onto on seedbed with the carbendazim aqueous solution and the root-growing agent aqueous solution after blinding again, and waters sufficient normal root water.
Further, described step admixes bactericide and insecticide after (4) ploughing deeply soil, and is exposed to the sun 3 days.
Further, (5) described step is tamed management and is comprised temperature treatment, when noon, external temperature was too high, carries out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
Beneficial effect of the present invention:
1, the component of plantlet in vitro domestication matrix of the present invention is made up of sandy soil or folder sandy soil, humus soil and wood sawdust, and the soil permeability of this matrix is good, is conducive to again the growth of bletilla plantlet in vitro.Clean plantlet in vitro is one clump with 3 by transplanting method, and by the mode surface planting of row green onion on seedbed, such transplanting method improves plantlet in vitro survival rate.This is because the survival rate that the survival rate of single planting kind does not have several to plant together is high, and bletilla pseudobulb form is to arrange the mode surface planting of green onion to seedbed, is more conducive to its growth than upright plantation.
2, the present invention provides an applicable transition method from test bottle to the Nature for bletilla plantlet in vitro, make that plantlet in vitro is very enough improves the absorption function of its coring, the photosynthesis function of dredging transportation function, blade of cane very soon, can reform of nature environment quickly, improve the transplanting survival rate of plantlet in vitro, application the method, can make domestication transplantation of seedlings survival rate to more than 95%.
Embodiment
Embodiment 1
A hardening cultivation method for bletilla plantlet in vitro, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by folder sandy soil, humus soil and wood sawdust according to weight ratio 3:2:1 mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then the blade of plantlet in vitro is covered with wet towel, and only root is exposed, finally root is placed in 1000 times of carbendazim aqueous solution and soaks 2 minutes, transfer in 1000 times of root-growing agent aqueous solution to soak after 3 minutes and wait for transplanting;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, admixes bactericide and insecticide mixes, and sees that insect catches and kill in process of digging, and 3 days effects of being exposed to the sun are better, and ditching, ridging makes seedbed; Then plantlet in vitro step (3) cleaned transplants the seedbed to booth, clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding, and then be evenly sprayed onto on seedbed with the carbendazim aqueous solution and the root-growing agent aqueous solution, and water sufficient normal root water;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 4 months; When noon, external temperature was too high, carry out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
Wherein, booth does traditional cements framework booth, the shade net of lid layer horticultural covering film and one deck shade density 80%, erection sprinkling irrigation system, and the fly net of 50 centimetres high is established in bottom, booth four sides, hangs up properly the yellow insect protected paster of suitable density in canopy.
In the present embodiment, transplant plantlet in vitro 300, finally survive 294, survival rate 98.1%.
Embodiment 2
A hardening cultivation method for bletilla plantlet in vitro, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by sandy soil, humus soil and wood sawdust according to weight ratio 1:1:1 mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then the blade of plantlet in vitro is covered with wet towel, and only root is exposed, finally root is placed in 1000 times of carbendazim aqueous solution and soaks 2 minutes, transfer in 1000 times of root-growing agent aqueous solution to soak after 3 minutes and wait for transplanting;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, admixes bactericide and insecticide mixes, and sees that insect catches and kill in process of digging, and 3 days effects of being exposed to the sun are better, and ditching, ridging makes seedbed; Then the plantlet in vitro step (3) cleaned transplants the seedbed to booth, and clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 7 months; When noon, external temperature was too high, carry out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
Wherein, booth does traditional cements framework booth, the shade net of lid layer horticultural covering film and one deck shade density 80%, erection sprinkling irrigation system, and the fly net of 50 centimetres high is established in bottom, booth four sides, hangs up properly the yellow insect protected paster of suitable density in canopy.
In the present embodiment, transplant plantlet in vitro 300, finally survive 287, survival rate 95.8%.
Embodiment 3
A hardening cultivation method for bletilla plantlet in vitro, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by folder sandy soil, humus soil and wood sawdust according to weight ratio 2:2:1 mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then the blade of plantlet in vitro is covered with wet towel, and only root is exposed, finally root is placed in 1000 times of carbendazim aqueous solution and soaks 2 minutes, transfer in 1000 times of root-growing agent aqueous solution to soak after 3 minutes and wait for transplanting;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, admixes bactericide and insecticide mixes, and sees that insect catches and kill in process of digging, and 3 days effects of being exposed to the sun are better, and ditching, ridging makes seedbed; Then the plantlet in vitro step (3) cleaned transplants the seedbed to booth, and clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 5 months; When noon, external temperature was too high, carry out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
Wherein, booth does traditional cements framework booth, the shade net of lid layer horticultural covering film and one deck shade density 80%, erection sprinkling irrigation system, and the fly net of 50 centimetres high is established in bottom, booth four sides, hangs up properly the yellow insect protected paster of suitable density in canopy.
In the present embodiment, transplant plantlet in vitro 300, finally survive 290, survival rate 96.7%.
Embodiment 4
A hardening cultivation method for bletilla plantlet in vitro, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by folder sandy soil, humus soil and wood sawdust according to weight ratio 1:2:2 mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then the blade of plantlet in vitro is covered with wet towel, and only root is exposed, finally root is placed in 1000 times of carbendazim aqueous solution and soaks 2 minutes, transfer in 1000 times of root-growing agent aqueous solution to soak after 3 minutes and wait for transplanting;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, admixes bactericide and insecticide mixes, and sees that insect catches and kill in process of digging, and 3 days effects of being exposed to the sun are better, and ditching, ridging makes seedbed; Then the plantlet in vitro step (3) cleaned transplants the seedbed to booth, and clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 6 months; When noon, external temperature was too high, carry out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
Wherein, booth does traditional cements framework booth, the shade net of lid layer horticultural covering film and one deck shade density 80%, erection sprinkling irrigation system, and the fly net of 50 centimetres high is established in bottom, booth four sides, hangs up properly the yellow insect protected paster of suitable density in canopy.
In the present embodiment, transplant plantlet in vitro 300, finally survive 292, survival rate 97.4%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a hardening cultivation method for bletilla plantlet in vitro, is characterized in that, comprise substrate preparation, in-bottle seeding, bottle outlet, domesticating and cultivating and domestication management, concrete steps are as follows:
(1) substrate preparation: by sandy soil or folder sandy soil, humus soil and wood sawdust mix make plantlet in vitro domestication matrix for subsequent use;
(2) in-bottle seeding: bletilla plantlet in vitro to be cultivated is moved in booth together with seedling bottle and carries out hardening, place 10-15 days under indoor natural light, then bottle cap is opened, place 1 day;
(3) bottle outlet: have the tweezers of cotton to take out plantlet in vitro by head bundle and the medium of its root is cleaned, making plantlet in vitro be divided into individual plant as far as possible; Cleaned plantlet in vitro is put into ventilating and cooling place and places a few hours, then cover the blade of plantlet in vitro with wet towel, and only root is exposed, finally root is placed in the carbendazim aqueous solution and soaks several minutes, transfer in the root-growing agent aqueous solution and soak several minutes;
(4) domesticating and cultivating: domestication matrix step (1) prepared evenly is sowed in booth, and carry out ploughing deeply soil, ditching, ridging makes seedbed; Then the plantlet in vitro step (3) cleaned transplants the seedbed to booth, and clean plantlet in vitro is one clump with 3 by transplanting method, by the mode surface planting of row green onion on seedbed, and then blinding;
(5) tame management: water weekly once permeable, topsoil only need be kept every day moistening and spray, plantlet in vitro transplants the dead seedling of cleaning after a week, transplant after 15 days, carry out single-dose application and weeding, transplant and execute foliage fertilizer once after one month, and spray an insecticide, the domestication time is 4-7 month.
2. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, is characterized in that, described sandy soil or folder sandy soil, humus soil and wood sawdust by weight proportioning are (1 ~ 3): (1 ~ 2): (1 ~ 2).
3. the hardening cultivation method of bletilla plantlet in vitro according to claim 2, is characterized in that, described sandy soil or folder sandy soil, humus soil and wood sawdust by weight proportioning are 3:2:1.
4. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, is characterized in that, described step bletilla plantlet in vitro (2) to be cultivated spends the hardening phase at other booths, then only need put in this booth and just can transplant for 2 days.
5. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, it is characterized in that, described step plantlet in vitro root (3) soaks 2 minutes with in 1000 times of carbendazim aqueous solution, transfers in 1000 times of root-growing agent aqueous solution to soak after 3 minutes to wait for transplanting.
6. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, is characterized in that, (4) described step is evenly sprayed onto on seedbed with the carbendazim aqueous solution and the root-growing agent aqueous solution after blinding again, and waters sufficient normal root water.
7. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, is characterized in that, described step admixes bactericide and insecticide after (4) ploughing deeply soil, and is exposed to the sun 3 days.
8. the hardening cultivation method of bletilla plantlet in vitro according to claim 1, is characterized in that, (5) described step is tamed management and comprised temperature treatment, when noon, external temperature was too high, carries out aeration-cooling; When winter, external temperature was lower, on seedbed, Small plastic shed insulation taken by cover.
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Cited By (17)
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| CN104904488A (en) * | 2015-06-30 | 2015-09-16 | 普洱良宝生物科技有限公司 | Cultivation method of bletilla striata seedlings |
| CN104938170A (en) * | 2015-05-27 | 2015-09-30 | 贵州省农作物品种资源研究所 | Greenhouse cultivation method for rhizoma bletillae seed stems |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN105453942A (en) * | 2015-12-09 | 2016-04-06 | 遵义市黔北绿野科技产业有限责任公司 | Method for increasing survival rate of hardening seedlings of rhizoma bletillae tissue culture seedlings |
| CN105532218A (en) * | 2015-12-14 | 2016-05-04 | 安徽世纪绿药生态农林有限公司 | Bletilla striata planting technology |
| CN105557270A (en) * | 2015-12-01 | 2016-05-11 | 贵州省农作物品种资源研究所 | Saddle-type bletilla striata tissue culture seed stem planting method |
| CN106305055A (en) * | 2016-08-18 | 2017-01-11 | 安徽省彩枫农林科技有限公司 | Domestication method for rhizoma bletillae seedlings |
| CN106342527A (en) * | 2016-08-27 | 2017-01-25 | 房县九州通药用植物科技有限公司 | Greenhouse cultivation method for tissue cultured seedling of bletilla striata |
| CN106417027A (en) * | 2016-10-14 | 2017-02-22 | 贵州万重山生态农业开发有限公司 | Low-temperature seedling hardening method for bletilla striata tissue culture seedlings |
| CN106613264A (en) * | 2016-12-29 | 2017-05-10 | 铜仁昱丰农业发展有限公司 | Bletilla striata tissue culture seedling transplantation and acclimation technology |
| CN106688848A (en) * | 2016-11-29 | 2017-05-24 | 云南省农业科学院药用植物研究所 | Efficient exercising technique for Bletilla striata tissue culture seedlings |
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN107493916A (en) * | 2017-09-25 | 2017-12-22 | 贵州大学 | A kind of hardening off method of bamboo shoot orchid tissue culture seedling |
| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
| CN115191310A (en) * | 2022-07-14 | 2022-10-18 | 西藏自治区高原生物研究所 | Seedling group hardening method for orchids |
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| CN104938170A (en) * | 2015-05-27 | 2015-09-30 | 贵州省农作物品种资源研究所 | Greenhouse cultivation method for rhizoma bletillae seed stems |
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| CN108770623A (en) * | 2018-06-23 | 2018-11-09 | 云南省农业科学院农业环境资源研究所 | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature |
| CN108901720A (en) * | 2018-06-23 | 2018-11-30 | 云南省农业科学院农业环境资源研究所 | Bletilla tissue culture seedlings waveform seedling bed hardening off method |
| CN114667857A (en) * | 2022-02-08 | 2022-06-28 | 贵州省农作物品种资源研究所 | Bletilla striata seedling domestication optimization method and domesticated bletilla striata seedlings |
| CN115191310A (en) * | 2022-07-14 | 2022-10-18 | 西藏自治区高原生物研究所 | Seedling group hardening method for orchids |
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