CN104528955B - A kind of microorganism formulation for repairing chemical fertilizer factory's sewage - Google Patents
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage Download PDFInfo
- Publication number
- CN104528955B CN104528955B CN201510038477.3A CN201510038477A CN104528955B CN 104528955 B CN104528955 B CN 104528955B CN 201510038477 A CN201510038477 A CN 201510038477A CN 104528955 B CN104528955 B CN 104528955B
- Authority
- CN
- China
- Prior art keywords
- atcc
- microorganism formulation
- prepare
- sewage
- microbe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 39
- 239000010865 sewage Substances 0.000 title claims abstract description 37
- 244000005700 microbiome Species 0.000 title claims abstract description 36
- 238000009472 formulation Methods 0.000 title claims abstract description 31
- 239000000126 substance Substances 0.000 title claims abstract description 26
- 239000003337 fertilizer Substances 0.000 title claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 16
- 239000003463 adsorbent Substances 0.000 claims abstract description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 17
- 241000209094 Oryza Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 239000012467 final product Substances 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 239000010902 straw Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 241000194032 Enterococcus faecalis Species 0.000 claims description 13
- 241000191938 Micrococcus luteus Species 0.000 claims description 13
- 241000187563 Rhodococcus ruber Species 0.000 claims description 13
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 13
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 12
- 244000105624 Arachis hypogaea Species 0.000 claims description 12
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 12
- 235000018262 Arachis monticola Nutrition 0.000 claims description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 235000012054 meals Nutrition 0.000 claims description 12
- 235000020232 peanut Nutrition 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 241000589291 Acinetobacter Species 0.000 claims description 10
- 229910021536 Zeolite Inorganic materials 0.000 claims description 9
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 9
- 239000004021 humic acid Substances 0.000 claims description 9
- 239000000377 silicon dioxide Substances 0.000 claims description 9
- 235000012239 silicon dioxide Nutrition 0.000 claims description 9
- 239000010457 zeolite Substances 0.000 claims description 9
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 8
- 229920001661 Chitosan Polymers 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 8
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 241000588626 Acinetobacter baumannii Species 0.000 claims description 3
- 241001524201 Arthrobacter crystallopoietes Species 0.000 claims description 3
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 12
- 150000001299 aldehydes Chemical class 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 239000003344 environmental pollutant Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 231100000719 pollutant Toxicity 0.000 description 8
- 238000004062 sedimentation Methods 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 238000005842 biochemical reaction Methods 0.000 description 4
- 238000012851 eutrophication Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 239000004576 sand Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical class [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to microbial technology field, disclose a kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms: step 1) prepare adsorbent, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microorganism formulation.Microorganism formulation of the present invention can remove ammonia nitrogen, SS and aldehydes matter in chemical fertilizer factory's sewage effectively, and removal effect is preferable, reaches discharge standard.
Description
Technical field
The invention belongs to microbial technology field, disclose a kind of microorganism formulation for repairing chemical fertilizer factory's sewage and application thereof.
Background technology
Chemical fertilizer factory's sewage is the important sources of trade effluent, and the pollutant index that mainly exceeds standard is ammonia nitrogen, sulfide and aldehydes matter.In sewage, all kinds of pollutant loads are high, belong to high pollution thing sewage, and ammonia nitrogen concentration height can reach more than 1000mg/L up to 700mg/L, COD, and sulfide can reach 100mg/L, and phenols can reach 50mg/L.At present, method to high density pollution thing sewage disposal is divided into physico-chemical process, biochemical combination method, novel biological dinitrification method and traditional biological denitrogenation both at home and abroad.At present, more physico-chemical process, novel biological dinitrification method and traditional biological denitrogenation is studied.
Ammonia nitrogen is to cause body eutrophication and pollute the important polluter of one of environment, and in the almost all of Polluted water area of current China, ammonia nitrogen is all one of main pollutant.Body eutrophication has endangered all conglomeraties such as agricultural, fishery, tourist industry, also drinking water hygiene and food safety is constituted huge threat.Water pollution particularly body eutrophication has become as the influence factor that China's economic development is important.Often containing ammonia nitrogen in high density in chemical fertilizer industry sewage, it it is one of ammonia nitrogen in sewage main source.Therefore, how to remove the ammonia nitrogen in chemical fertilizer industry sewage and become a major issue in current Industrial Waste Water Treatments.Ammonia nitrogen is the major pollutants of chemical fertilizer factory's sewage, enters water body and can cause body eutrophication, causes water quality deterioration, make discharge severely limited.Chemical fertilizer factory's sewage is essentially from synthesis ammonia, the high concentration ammonia nitrogen sewage of urea plant, and it is inorganic ammonia that this part of contaminated water ammonia nitrogen is primarily present form.In sum, along with expanding economy, the ambient pressure that chemical fertilizer factory faces is increasingly strict, and for the needs of sustained economic development, in actual production, sewage effluent becomes giving top priority to what is the most important of chemical fertilizer factory's environmental problem.
For a long time, biochemical sewage processes adding of carbon source and uses methanol, white sugar (or cerelose) always, and methanol belongs to harmful influence, does not the most only exist the biggest danger；White sugar is relatively costly, and enterprise is the most beyond affordability.The microorganism formulation mechanism of action in terms of environmental conservation is mainly: pollutant oxidation is decomposed into the end-product such as carbon dioxide and water by metabolic response or is converted into the nutrient substance of microorganism by microbial inoculum; promote the growth and breeding of self, just as H2S can be changed into the element sulphur required for own growth by photosynthetic bacteria and bacillus cereus etc..Prior art processes the microorganism formulation of chemical fertilizer factory sewage, and mostly to there is bacteria microorganism compatibility unreasonable, and removal efficiency is low, and and relatively costly, enterprise is difficult to the defects such as acceptance, significantly hinders the application process of Biochemical method sewage.
Summary of the invention
The technical issues that need to address of the present invention are, in place of overcoming the deficiencies in the prior art, provide a kind of microorganism formulation for repairing chemical fertilizer factory's sewage and application thereof, this microorganism formulation comprises multiple bacterial strain, compatibility is reasonable, and synergism is strong, it is possible to effectively remove the ammonia nitrogen in sewage, sulfide, aldehydes matter and industry COD, low in input cost, can large-scale promotion use.
It is an object of the invention to be achieved through the following technical solutions:
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms: step 1) prepare adsorbent, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microorganism formulation.
Specifically, described microorganism formulation is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain particle diameter zeolite powder between 50-100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product；
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer；Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer；Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product；The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5；
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product；
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20-22 DEG C, and dried water content is 10-12%, to obtain final product；The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Preferably,
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442；
Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212；
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906；
Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606；
The brilliant arthrobacterium of described one-tenth is for becoming brilliant arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481；
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842；
Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
The particle diameter of described silicon dioxide or humic acids is 50-100 mesh.
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7-7.5, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Strain of the present invention all can be commercially available from commercial channel such as American Type Culture collection warehousing (ATCC).The amplification culture method of the bacterial strain of the present invention can record according to document or the record of prior art is carried out, and is not the most the innovative point of the present invention, as space is limited, repeats the most one by one.
The beneficial effect that the present invention obtains is primarily but not limited to the following aspects:
Reasonable compatibility between each strain in microorganism formulation of the present invention, symbiosis coordination, the most not antagonism, activity is high, and Biomass is big, and breeding is soon；The microorganism formulation of the present invention, by the various strains that can form dominant microflora, is configured to high-effect bacterial, is added in Waste Water Treatment by a certain amount of, accelerates the bacterial strain degraded to pollutant, to improve the biological treatment efficiency of system, it is ensured that system stable operation；Microorganism formulation of the present invention is suitable to chemical fertilizer factory's sewage disposal, can improve the process water yield and water quality treatment, reduces operating cost, promotes qualified discharge；The present invention is prepared for microbe carbon source nutriment, low price by agricultural wastes, is easily accept by enterprises for；The present invention with the addition of the adsorbent that the silicon dioxide of certain particle diameter, zeolite powder and humic acids are constituted, and can be used to assemble pollutant and thalline, improves the contact area of bacterial strain and pollutant, improves biological oxidation efficiency.
Detailed description of the invention
For the technical scheme making those skilled in the art be more fully understood that in the application, below in conjunction with the application specific embodiment, the technical scheme of the application is clearly and completely described, it is clear that, described embodiment is only some embodiments of the present application rather than whole embodiments.Based on the embodiment in the application, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all should belong to the scope of protection of the invention.
Embodiment 1
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain the zeolite powder that particle diameter is 50, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product；The particle diameter of described silicon dioxide or humic acids is 50 mesh.
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer；Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer；Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor at 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product；The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5；
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product；
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20 DEG C, and dried water content is 10%, to obtain final product；The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Embodiment 2
A kind of microorganism formulation for repairing chemical fertilizer factory's sewage, it is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain the particle diameter zeolite powder at 100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product；The particle diameter of described silicon dioxide or humic acids is 100 mesh；
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer；Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer；Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor at 100 DEG C, insulation reaction 30 minutes, last natural cooling and get final product；The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5；
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product；
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 22 DEG C, and dried water content is 12%, to obtain final product；The mass ratio of described adsorbent, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Described micrococcus luteus be micrococcus luteus (Micrococcus luteus) ATCC 49442 (APPLIED AND ENVIRONMENTAL MICROBIOLOGY1992, p.3423-3425)；
Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212 (In Vivo.2009 Jan-Feb；23 (1): 81-7.)；
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906 (J.Bacteriol.November 2001)；
Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606 (Infect Immun.2012Mar；80 (3): 1015-24)；
The brilliant arthrobacterium of described one-tenth is finished product arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481 (Appl.Environ.Microbiol.March 15,200975:61774-1777).
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842 (J.Microbiol.Biotechnol. (2006), 16 (10), 1650-1655)；
Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC49642 (WO2003066873A1).
The using method of mentioned microorganism preparation includes: first remove the float in sewage, subsequently into sedimentation tank, sedimentation tank is laid the fine sand of 50cm thickness, is precipitated 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microorganism formulations according to every cubic metre of liquid every time, adds 1 every day, after adding three days continuously, stand one week again, by liquid filtration sterilization, finally discharge.
Embodiment 3
With the sewage of this city chemical fertilizer factory as example, first remove the float in sewage, subsequently into sedimentation tank, the fine sand of 50cm thickness laid by sedimentation tank, precipitating 12 hours, subsequently enter biochemical reaction tank, the pH adding hydrochloric acid adjustment sewage is 7, add 20 grams of microorganism formulations according to every cubic metre of liquid every time, add every day 1 time, after adding three days continuously, then stand one week, by liquid filtration sterilization, finally discharge.Detection all takes three water samples every time, averages；The most each index all reaches preferable discharge standard, and the Testing index of sewage is shown in Table 1:
Table 1
Conclusion: microorganism formulation of the present invention can remove ammonia nitrogen, SS and aldehydes matter in chemical fertilizer factory's sewage effectively, removal effect is preferable, reaches discharge standard.
Although, the most illustrating by generality and detailed explanation has been made in this case by detailed description of the invention, but on the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, the amendment made without departing from theon the basis of the spirit of the present invention or improvement, belong to the scope of protection of present invention.
Claims (3)
1. the microorganism formulation being used for repairing chemical fertilizer factory's sewage, it is characterised in that described microorganism formulation is prepared as follows and forms:
Step 1) prepare adsorbent: first zeolite is carried out pulverization process, obtain particle diameter zeolite powder between 50-100 mesh, then mix homogeneously according to the weight ratio of 1: 2: 2 with silicon dioxide, humic acids, to obtain final product；
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer；Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer；Above-mentioned rice straw powder and peanut hull meal are added in agitator tank, then bean cake and water are added, after the speed of 500 revs/min stirs 15 minutes, material in agitator tank is sent in reactor, it is passed through water vapour in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last natural cooling and get final product；The mass ratio of rice straw powder, peanut hull meal, bean cake and water is 2: 2: 3: 5；
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 mesh, then by chitosan, trehalose, kieselguhr and glycerol according to 1: 1: 1: 2 quality than mix homogeneously, to obtain final product；
Step 4) prepare microorganism formulation: micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, one-tenth crystalline substance arthrobacterium, many glutinous series bacillus and pseudomonas fluorescens are trained concentration respectively is 1 × 108The bacterium solution of individual/ml, according to 7: 5: 5: the volume ratio mixing of 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours, add step 1 subsequently for 30 DEG C) adsorbent prepared and step 3) microbe carrier prepared, 100 turns/min stirs 3min, finally carry out cold drying, baking temperature is 20-22 DEG C, and dried water content is 10-12%, to obtain final product；
Described step 4) in, the mass ratio of adsorbent, microbe carrier and microbe carbon source is 1: 2: 5.
Microorganism formulation the most according to claim 1, it is characterised in that described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442；Described enterococcus faecalis be enterococcus faecalis (Enterococcus faecalis) be ATCC 29212；Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906；Described Acinetobacter bauamnnii be Acinetobacter bauamnnii (Acinetobacter baumanii) be ATCC 19606；The brilliant arthrobacterium of described one-tenth is for becoming brilliant arthrobacterium (Arthrobacter crystallopoietes) ATCC 15481；Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842；Described pseudomonas fluorescens is pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
Microorganism formulation the most according to claim 1, it is characterised in that the particle diameter of described silicon dioxide or humic acids is 50-100 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510038477.3A CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510038477.3A CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104528955A CN104528955A (en) | 2015-04-22 |
| CN104528955B true CN104528955B (en) | 2016-08-24 |
Family
ID=52844649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510038477.3A Expired - Fee Related CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104528955B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475161A (en) * | 2017-09-25 | 2017-12-15 | 黄灵君 | The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132345A (en) * | 2015-10-27 | 2015-12-09 | 淮海工学院 | Enterococcus sp. Y12 and application thereof |
| CN106148223B (en) * | 2016-05-06 | 2020-04-21 | 国家海洋局第一海洋研究所 | Arctic domestic sewage low-temperature efficient degrading bacterium and screening method and application thereof |
| CN106862260A (en) * | 2017-03-10 | 2017-06-20 | 刘晨 | A kind of biochemical process for administering Cadmium Pollution in Soils |
| CN106929453B (en) * | 2017-04-11 | 2020-06-26 | 内蒙古阜丰生物科技有限公司 | Biological agent for treating monosodium glutamate fermentation mother liquor and preparation method thereof |
| CN106964647A (en) * | 2017-06-06 | 2017-07-21 | 临沂大学 | A kind of method of utilization composite bacteria agent remediating lead-contaminated soil |
| CN107446862B (en) * | 2017-09-06 | 2023-12-15 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading pesticide residues and preparation method thereof |
| CN107446861B (en) * | 2017-09-06 | 2024-02-27 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading heavy metal residues and preparation method thereof |
| CN107557318B (en) * | 2017-09-25 | 2021-07-20 | 杭州富阳佳畅机械有限公司 | Microbial preparation for removing hydrogen sulfide malodorous substance |
| CN109650642A (en) * | 2017-10-11 | 2019-04-19 | 卢松 | A kind of environment-protective process that xanthan gum industrial wastewater is effectively treated |
| CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
| CN110902849B (en) * | 2020-02-10 | 2020-07-03 | 广州赛太特生物医学科技有限公司 | Method for treating industrial wastewater by using biological adsorbent |
| CN110921850B (en) * | 2020-02-10 | 2020-06-05 | 广州赛太特生物医学科技有限公司 | Method for treating sewage by using microorganisms |
| CN112607954A (en) * | 2020-11-24 | 2021-04-06 | 安徽广信农化股份有限公司 | Novel process for reducing hexazinone wastewater |
| CN115044580A (en) * | 2022-08-03 | 2022-09-13 | 广州微立旺生物科技有限公司 | Compound microbial agent for removing sulfide and preparation method thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6325934B1 (en) * | 2000-05-09 | 2001-12-04 | James Francis Tobey, Jr. | Enzyme and bacterial combination in a slowly dissolvable matrix for septic tanks, grease traps and waste treatment |
| CN103409404B (en) * | 2013-08-23 | 2014-12-10 | 山东元通监测有限公司 | Biological preparation for treating domestic sewage |
| CN103739085A (en) * | 2013-12-20 | 2014-04-23 | 大连赛姆生物工程技术有限公司 | Holothurian culture water body remediation agent prepared by mixed fermentation process and preparation method and application thereof |
| CN104152374A (en) * | 2014-07-10 | 2014-11-19 | 河南恒野农牧股份有限公司 | Microbial preparation for purifying breeding wastewater |
-
2015
- 2015-01-22 CN CN201510038477.3A patent/CN104528955B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475161A (en) * | 2017-09-25 | 2017-12-15 | 黄灵君 | The technique for removing hydrogen sulfide contamination thing in house refuse using microorganism formulation |
| CN107475161B (en) * | 2017-09-25 | 2021-07-20 | 杭州富阳佳畅机械有限公司 | Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104528955A (en) | 2015-04-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104528955B (en) | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage | |
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN104478098B (en) | For the treatment of the microbial preparation of coal chemical industrial waste water | |
| Das et al. | Microalgal nutrients recycling from the primary effluent of municipal wastewater and use of the produced biomass as bio-fertilizer | |
| CN104630101B (en) | It is a kind of to be used to handle biological agent of ammonia-nitrogen sewage and preparation method thereof | |
| CN102888374B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN102888345B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN103373762B (en) | Biological denitrification method for salt-containing sewage | |
| CN106754461B (en) | Biological floc and preparation method and application thereof | |
| CN103449680B (en) | Method for removing pollutants in sewage | |
| CN104086001B (en) | Treatment process applicable to industrial wastewater and application of industrial wastewater in preparation of bacterial manure | |
| CN104528956B (en) | A kind of composite bacteria agent capable for administering coal chemical industry sewage | |
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| CN104628225B (en) | A kind of processing method containing ammonia nitrogen industrial wastewater | |
| CN103740764A (en) | Functional biocatalyst for biogas fermentation and method for fermenting using catalyst | |
| CN102430142A (en) | Composite ecological deodorant for controlling malodorous gas | |
| CN105060495A (en) | Biological agent for treatment of high ammonia-nitrogen industrial sewage | |
| CN104973696A (en) | Preparation technology of biological agent for restoring industrial sewage | |
| CN104891678A (en) | Biological agent used for treating high-concentration waste water produced by amino acid fermentation | |
| CN105000674A (en) | Method used for processing environmental geology industrial wastewater via biotechnology | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN105060508A (en) | Biological sewage-treatment complex microbial inoculant and preparation method thereof | |
| CN105152478B (en) | Treatment method of industrial wastewater produced during preparation of sodium glutamate through concentration isoelectric process | |
| CN104496114B (en) | A kind of environment-protective process processing coal chemical industry sewage | |
| CN104496118B (en) | A kind of method of disposing of sewage in Coal Chemical Industry synthetic ammonia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Cao Junping Inventor before: Gong Yanhua Inventor before: Chen Jing |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160413 Address after: 276001 room 23, environmental protection bureau, No. 512, Beijing Road, Linyi new North District, Shandong Applicant after: Cao Junping Address before: 276001 room 23, environmental protection bureau, No. 506, Beijing Road, Linyi new North District, Shandong Applicant before: Gong Yanhua |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Wei Inventor after: Zhao Fei Inventor before: Cao Junping |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170927 Address after: 063000 Hebei Tangshan City Road North District Construction North Road Jianshe Road Red Star Building 44 Building 1 gate 203 Co-patentee after: Zhao Fei Patentee after: Liu Wei Address before: 276001 room 23, environmental protection bureau, No. 512, Beijing Road, Linyi new North District, Shandong Patentee before: Cao Junping |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180420 Address after: 310018 No. 4 standard workshop No. 1, No. 1, 8 Avenue, Hangzhou economic and Technological Development Zone, Hangzhou, Zhejiang. Patentee after: Hangzhou lubricant Science Equipment Co., Ltd. Address before: 063000 Hebei Tangshan City Road North District Construction North Road Jianshe Road Red Star Building 44 Building 1 gate 203 Co-patentee before: Zhao Fei Patentee before: Liu Wei |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160824 Termination date: 20190122 |