CN104509683A - Method for preparing yellow corn silage feed by compounding and fermenting shells of bamboo shoots - Google Patents
Method for preparing yellow corn silage feed by compounding and fermenting shells of bamboo shoots Download PDFInfo
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- CN104509683A CN104509683A CN201410823883.6A CN201410823883A CN104509683A CN 104509683 A CN104509683 A CN 104509683A CN 201410823883 A CN201410823883 A CN 201410823883A CN 104509683 A CN104509683 A CN 104509683A
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Abstract
The invention discloses a method for preparing a yellow corn silage feed by compounding and fermenting shells of bamboo shoots. The method specially comprises the following operating steps: (1) crushing the shells of the bamboo shoots, adding a small amount of bran and a proper amount of water, mixing uniformly, and sterilizing to prepare a fermenting base material; (2) inoculating oyster mushroom strains into the fermenting base material, and culturing oyster mushroom mycelia under the conditions of suitable temperature and humidity; (3) inoculating lactobacilli into the fermenting material of oyster mushroom, performing anaerobic fermentation at a suitable temperature to generate lactic acid until the pH of the material is lower than 6.0, and completing the fermentation. According to a process, the method of aerobic fermentation and anaerobic fermentation in series is adopted; two microbes are respectively inoculated to serve as fermenting strains, so that the shells of the bamboo shoots which are difficult to degrade are converted into the yellow corn silage feed which is easily absorbed and utilized by animals; by the process, the economic benefits of a bamboo shoot food processing plant can be improved, and meanwhile, the pollution of surrounding environment caused by the plant can be reduced.
Description
Technical field
The invention belongs to bioengineering field, specifically utilize fermentation engineering, the leftover bits and pieces that bamboo shoot process produces is changed into the nutriment can digested and assimilated by animal further, realizes refuse reclamation, make recycling economy.
Background technology
Compositions of Bamboo Shoot Shell splits away off after bamboo shoots grow up to bamboo or the byproduct of bamboo shoots after processing.Cellulose in Compositions of Bamboo Shoot Shell, hemicellulose and content of lignin enrich, also containing nutriments such as sugar, total nitrogen, amino acid, but former bamboo shoot chitin ground is hard, palatability is poor, is is not easily digested and assimilated by animal, and fermentable can improve the palatability of bamboo shoot shell and improve feeding value.
China's bamboo grove area is wide, and annual bamboo shoot shell amount millions of ton of meter produced, the leftover bits and pieces bamboo shoot shell especially after bamboo shoots food processing, conducting oneself well as improper to put to become huge environomental pollution source.If be translated into long keeping animal feed by modern biotechnology, be then all of great advantage to energy-saving and emission-reduction, environmental protection, the fine rural area of construction.
Summary of the invention
In order to make the leftover bits and pieces of bamboo shoots food processing---Compositions of Bamboo Shoot Shell is fully used, and reduces environmental pollution simultaneously, the invention provides a kind of method Compositions of Bamboo Shoot Shell composite fermentation being made yellow storage feed.
The present invention's flat mushroom strain used and Lactobacillus plantarum bacterial classification are respectively purchased from China typical culture collection center and China General Microbiological culture presevation administrative center, and Classification system is respectively
pleurotus ostreatuswith
lactobacillus plantarum, preserving number is respectively: CCTCC AF 92003 and CGMCC 1.2566.
The operating procedure that preparation Compositions of Bamboo Shoot Shell composite fermentation makes yellow storage feed is as follows:
(1) Compositions of Bamboo Shoot Shell is pulverized, adds wheat bran and water, mix thoroughly, sterilizing, make fermentation bed material;
(2) in bed material, flat mushroom strain is accessed, aerobic fermentation, obtained oyster mushroom fermentation material;
(3) in oyster mushroom fermentation material, access lactic acid bacteria, anaerobic fermentation lactic acid producing lower than 6.0 to material pH, has been fermented and has been obtained fermentation materials;
(4) by fermentation materials, granulation, obtained Compositions of Bamboo Shoot Shell Huang storage feed, gained Huang storage feed is glassy yellow, and the loose softness of quality, distribute pleasant sour fragrance, pH value 5.0-6.0, crude protein content 15-16%, content of reducing sugar is less than 1%, crude fiber content 70-73%.
The concrete preparation manipulation step that Compositions of Bamboo Shoot Shell composite fermentation makes yellow storage feed is as follows:
(1) making of Compositions of Bamboo Shoot Shell fermentation bed material
Compositions of Bamboo Shoot Shell dried, pulverizes, cross 20 mesh sieves, obtain Compositions of Bamboo Shoot Shell powder, add wheat bran by the amount of Compositions of Bamboo Shoot Shell grain weight amount 1/4 ~ 1/5, then add water by the amount of Compositions of Bamboo Shoot Shell grain weight amount 2 ~ 2.5 times, mix thoroughly, steam sterilizing, obtain mixing bed material;
(2) aerobic fermentation obtains oyster mushroom fermentation material
In mixing bed material, access mushroom cultivation kind, inoculum concentration is 1 ~ 2% of Compositions of Bamboo Shoot Shell grain weight amount, and temperature 27 ± 2 DEG C, humidity 60 ~ 70%, cultivate 15 d, and mycelia is long close long real in material, obtains oyster mushroom fermentation material;
(3) anaerobic fermentation obtains fermentation materials
Oyster mushroom fermentation material is broken up, dried, access Lactobacillus plantarum bacteria suspension, inoculum concentration is 1.0-1.2 times of oyster mushroom fermentation siccative weight.Isolated air, temperature 40 DEG C cultivate 30 d, obtain fermentation materials;
(4) fermentation materials allotment
Fermentation materials is spread out and dries, control water content 40-50%, directly packaging or granulation, obtain Compositions of Bamboo Shoot Shell Huang storage feed.
Advantageous Effects of the present invention embodies in the following areas:
1. the Huang storage feed that the present invention obtains has nutritious, fragrant, quality softness, and good palatability, is easy to animal chew; This finished product can directly or grain ration as ruminant composite with other feed;
2. the whole operating process of preparation technology of the present invention is simple, and without the need to the production equipment of complexity, can gather materials on the spot, that suits measures to local conditions carries out the work, and is applicable to large-scale promotion;
3. wherein the effect of aerobic fermentation utilizes the lignocellulosic in white-rot fungi decomposition bamboo shoot shell.Flat mushroom is a kind of white-rot fungi, Pleurotus ostreatus can synthesize and secrete the cellulase of high vigor, hemicellulase and lignoenzyme in growth course, the small-molecule substance that the large molecular breakdown Cheng Yi of lignocellulose being difficult to degrade in Compositions of Bamboo Shoot Shell can be absorbed by organism by the synergy of these three kinds of enzymes; In Compositions of Bamboo Shoot Shell, add a small amount of wheat bran and appropriate moisture, sterilizing, then access flat mushroom strain, under suitable temperature and humidity condition, hypha of Pleurotus ostreatus spreads the lignocellulosic in growth decomposition bamboo shoot shell in a large number;
Lactic acid bacteria anaerobic fermentation lactic acid producing: organic containing a large amount of Small molecular in the Compositions of Bamboo Shoot Shell material that flat mushroom decomposes, especially the monose of some reproducibilities and oligosaccharides, lactic acid bacteria under anaerobic can produce lactic acid for decomposition-reduction sugar, lactic acid can reduce the pH value of material, suppress the growth of other miscellaneous bacteria, give material special fragranced simultaneously, increase the palatability of material;
Aerobic fermentation and anaerobic fermentation are cascaded by the inventive method, and the dry matter content in former Compositions of Bamboo Shoot Shell can be made after having fermented to reduce about 40%;
4. before fermentation process, the content of the crude fibre of Compositions of Bamboo Shoot Shell powder, crude protein, DDGS and reduced sugar is respectively: 73.8%, 12.7%, 26.2% and 1.28%, and pH value is in neutral; After this PROCESS FOR TREATMENT, in gained fermentation materials, the content of crude fibre, crude protein, DDGS and reduced sugar is respectively: 70-73%, 15-16%, 27-30% and 0.7-0.9%, pH value 5.0-6.0; In sweat, the consumption rate of total dry material is 26-30%.
Detailed description of the invention
Below by embodiment, the present invention is described in further detail.
The present invention's flat mushroom strain used and Lactobacillus plantarum bacterial classification are respectively purchased from China typical culture collection center and China General Microbiological culture presevation administrative center, and Classification system is respectively
pleurotus ostreatuswith
lactobacillus plantarum, preserving number is respectively: CCTCC AF 92003 and CGMCC 1.2566.
Embodiment 1
The concrete operation step that Compositions of Bamboo Shoot Shell composite fermentation makes yellow storage feed is as follows:
1, the making of Compositions of Bamboo Shoot Shell fermentation bed material
Take Compositions of Bamboo Shoot Shell 20 g dried, cross 20 mesh sieves, add 5 g wheat brans, then add 50 mL running water, mix thoroughly, load in 250 mL beakers, compacting after pulverizing, newspaper seals, tightens, 121 DEG C of steam sterilizing 30 min;
2, aerobic fermentation obtains oyster mushroom fermentation material
The flat mushroom strain getting Compositions of Bamboo Shoot Shell dried bean noodles weight 2% is placed in the surface of fermentation bed material, and temperature 28 DEG C, humidity 65-70%, cultivate 15 days, and mycelia is long close long real in material, stops cultivating;
The preparation method of flat mushroom seed: the vial waterishlogged grain of rice being filled 500 mL, with newspaper sealing, tighten, 121 DEG C of steam sterilizing 30 min, dig out the square fritter of one 5 mm by flat mushroom slant strains, move in vial, 28 DEG C of lucifuges are cultured to till mycelia covers with the grain of rice;
3, anaerobic fermentation obtains fermentation materials
By the hypha of Pleurotus ostreatus fermented group again break up, 60 DEG C of oven dry, add the lactobacillus solution of oyster mushroom fermentation material dry weight 1.2 times, mix thoroughly, fill the screw socket centrifuge tube that 50 mL are with cover, compacting, sealing, be placed in 40 DEG C of incubator 30 d and take out, and Compositions of Bamboo Shoot Shell Huang storage feed fermentation completes;
The preparation method of lactobacillus solution: get a ring culture with oese from Lactobacillus plantarum inclined-plane and put into 50 mL MRS fluid nutrient mediums, inoculation medium is placed in 40 DEG C of incubator quiescent culture 3 d lactic acid bacteria culture solution, get centrifugal 2 min of 5 mL lactic acid bacteria culture solution 12000 r/min, abandon supernatant, precipitation adds 10 mL sterilized water vibrations and beats, more centrifugal 2 min of 12000 r/min, abandons supernatant, precipitation adds 20 mL sterilized water vibrations and beats, and is namely " lactobacillus solution ";
MRS culture medium: peptone 10 g, beef extract 10 g, dusty yeast 5 g, K
2hPO
42 g, dibasic ammonium citrate 2 g, sodium acetate 5 g, glucose 20 g, Tween 80 1.0 mL, epsom salt (MgSO
47H
2o) 0.58g, four water manganese sulfate (MnSO
44H
2o) 0.25 g, distilled water 1000 mL;
4, fermentation materials is spread out dry, control water content 50%, granulation, obtained Compositions of Bamboo Shoot Shell Huang storage feed, gained Huang storage feed is glassy yellow, and the loose softness of quality, distributes pleasant sour fragrance, pH value 5.8, crude protein content 15.2%, content of reducing sugar 0.71%, crude fiber content 70%.
Embodiment 2
The concrete operation step that Compositions of Bamboo Shoot Shell composite fermentation makes yellow storage feed is as follows:
1, the making of Compositions of Bamboo Shoot Shell fermentation bed material:
Take Compositions of Bamboo Shoot Shell 150 g dried, cross 20 mesh sieves after pulverizing, add 30 g wheat brans, then add 300 mL running water, mix thoroughly, load in 1000 mL plastic lunch box with cover, compacting, cover lid, 121 DEG C of steam sterilizing 30 min;
2, aerobic fermentation obtains oyster mushroom fermentation material
Get the surface that flat mushroom strain that Compositions of Bamboo Shoot Shell dried bean noodles weighs 1% is placed in fermentation bed material, temperature 25 DEG C, humidity 60-65%, cultivate 15 d, and mycelia is long close long real in material, stops cultivating;
The preparation method of flat mushroom seed: the vial waterishlogged grain of rice being filled 500 mL, with newspaper sealing, tighten, 121 DEG C of steam sterilizing 30 min, dig out the square fritter of one 5 mm by flat mushroom slant strains, move in vial, 28 DEG C of lucifuges are cultured to till mycelia covers with the grain of rice;
3, anaerobic fermentation obtains fermentation materials
By the hypha of Pleurotus ostreatus fermented group again break up, 60 DEG C of oven dry, add the lactobacillus solution of oyster mushroom fermentation material dry weight 1.0 times, mix thoroughly, fill the screw socket centrifuge tube that 5 50 mL are with cover, compacting, sealing, be placed in 40 DEG C of incubator 30 d and take out, and Compositions of Bamboo Shoot Shell Huang storage feed fermentation completes;
The preparation method of lactobacillus solution: get a ring culture with oese from Lactobacillus plantarum inclined-plane and put into 50 mL MRS fluid nutrient mediums, inoculation medium is placed in 40 DEG C of incubator quiescent culture 3 d lactic acid bacteria culture solution, get centrifugal 2 min of 35 mL lactic acid bacteria culture solution 12000 r/min, abandon supernatant, precipitation adds 40 mL sterilized water vibrations and beats, be distributed into 4 pipes, centrifugal 2 min of 12000 r/min again, abandon supernatant, often pipe precipitation adds 35 mL sterilized waters vibrations and beats and mix, is namely " lactobacillus solution ";
MRS culture medium: peptone 10 g, beef extract 10 g, dusty yeast 5 g, K
2hPO
42 g, dibasic ammonium citrate 2 g, sodium acetate 5 g, glucose 20 g, Tween 80 1.0 mL, epsom salt (MgSO
47H
2o) 0.58g, four water manganese sulfate (MnSO
44H
2o) 0.25 g, distilled water 1000 mL;
4, spread out by fermentation materials and dry, control water content 40%, obtained Compositions of Bamboo Shoot Shell Huang storage feed, gained Huang storage feed is glassy yellow, the loose softness of quality, distributes pleasant sour fragrance, pH value 5.1, crude protein content 15.9%, content of reducing sugar 0.80%, crude fiber content 73%.
Claims (2)
1. Compositions of Bamboo Shoot Shell composite fermentation makes a method for yellow storage feed, it is characterized in that operating procedure is as follows:
(1) Compositions of Bamboo Shoot Shell is pulverized, adds wheat bran and water, mix thoroughly, sterilizing, make fermentation bed material;
(2) in bed material, flat mushroom strain is accessed, aerobic fermentation, obtained oyster mushroom fermentation material;
(3) in oyster mushroom fermentation material, access lactic acid bacteria, anaerobic fermentation lactic acid producing lower than 6.0 to material pH, has been fermented and has been obtained fermentation materials;
(4) by fermentation materials, granulation, obtained Compositions of Bamboo Shoot Shell Huang storage feed, gained Huang storage feed is glassy yellow, and the loose softness of quality, distribute pleasant sour fragrance, pH value 5.0-6.0, crude protein content 15-16%, content of reducing sugar is less than 1%, crude fiber content 70-73%.
2. a kind of Compositions of Bamboo Shoot Shell composite fermentation according to claim 1 makes the method for yellow storage feed, it is characterized in that concrete operation step is as follows:
(1) making of Compositions of Bamboo Shoot Shell fermentation bed material
Compositions of Bamboo Shoot Shell dried, pulverizes, cross 20 mesh sieves, obtain Compositions of Bamboo Shoot Shell powder, add wheat bran by the amount of Compositions of Bamboo Shoot Shell grain weight amount 1/4 ~ 1/5, then add water by the amount of Compositions of Bamboo Shoot Shell grain weight amount 2 ~ 2.5 times, mix thoroughly, steam sterilizing, obtain mixing bed material;
(2) aerobic fermentation obtains oyster mushroom fermentation material
In mixing bed material, access mushroom cultivation kind, inoculum concentration is 1 ~ 2% of Compositions of Bamboo Shoot Shell grain weight amount, and temperature 27 ± 2 DEG C, humidity 60 ~ 70%, cultivate 15 d, and mycelia is long close long real in material, obtains oyster mushroom fermentation material;
(3) anaerobic fermentation obtains fermentation materials
Oyster mushroom fermentation material is broken up, dried, access Lactobacillus plantarum bacteria suspension, inoculum concentration is 1.0-1.2 times of oyster mushroom fermentation siccative weight; Isolated air, temperature 40 DEG C cultivate 30 d, obtain fermentation materials;
(4) fermentation materials allotment
Fermentation materials is spread out and dries, control water content 40-50%, directly packaging or granulation, obtain Compositions of Bamboo Shoot Shell Huang storage feed.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104920902A (en) * | 2015-07-01 | 2015-09-23 | 浙江农林大学 | Sheep feed based on bamboo shoot shells |
CN106173295A (en) * | 2016-07-12 | 2016-12-07 | 南宁学院 | A kind of ruminant feed made with Broussonetia papyrifera and preparation method thereof |
CN106343165A (en) * | 2016-08-26 | 2017-01-25 | 兴润生态(大连)生物基质处理有限公司 | Industrialized production method of yellow corn silage fermented feed |
CN106578419A (en) * | 2016-11-08 | 2017-04-26 | 浙江省农业科学院 | Method for processing feed by using bamboo shoot shell |
CN109497471A (en) * | 2018-12-29 | 2019-03-22 | 广西壮族自治区农业科学院农产品加工研究所 | Fine nutrient powder of height with intestinal regulatory function and preparation method thereof |
CN115606689A (en) * | 2022-10-12 | 2023-01-17 | 湖南农业大学 | A kind of solid fermentation method of bamboo shoot processing waste fermented feed |
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CN102125167A (en) * | 2010-12-30 | 2011-07-20 | 浙江大学 | Method for preparing bamboo shell feed by using waste bamboo shells |
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KANJI OKANO等: "Improving the nutritive value of madake bamboo,", 《ANIMAL FEED SCIENCE AND TECHNOLOGY》 * |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104920902A (en) * | 2015-07-01 | 2015-09-23 | 浙江农林大学 | Sheep feed based on bamboo shoot shells |
CN104920902B (en) * | 2015-07-01 | 2019-04-26 | 浙江农林大学 | A sheep feed based on bamboo shoots |
CN106173295A (en) * | 2016-07-12 | 2016-12-07 | 南宁学院 | A kind of ruminant feed made with Broussonetia papyrifera and preparation method thereof |
CN106343165A (en) * | 2016-08-26 | 2017-01-25 | 兴润生态(大连)生物基质处理有限公司 | Industrialized production method of yellow corn silage fermented feed |
CN106578419A (en) * | 2016-11-08 | 2017-04-26 | 浙江省农业科学院 | Method for processing feed by using bamboo shoot shell |
CN109497471A (en) * | 2018-12-29 | 2019-03-22 | 广西壮族自治区农业科学院农产品加工研究所 | Fine nutrient powder of height with intestinal regulatory function and preparation method thereof |
CN109497471B (en) * | 2018-12-29 | 2022-05-31 | 广西壮族自治区农业科学院农产品加工研究所 | High-fiber nutrition powder with intestinal tract adjusting function and preparation method thereof |
CN115606689A (en) * | 2022-10-12 | 2023-01-17 | 湖南农业大学 | A kind of solid fermentation method of bamboo shoot processing waste fermented feed |
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Application publication date: 20150415 |