CN104397323A - Microbial feed additive - Google Patents
Microbial feed additive Download PDFInfo
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- CN104397323A CN104397323A CN201410434316.1A CN201410434316A CN104397323A CN 104397323 A CN104397323 A CN 104397323A CN 201410434316 A CN201410434316 A CN 201410434316A CN 104397323 A CN104397323 A CN 104397323A
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Abstract
The invention relates to a microbial feed additive and belongs to the field of an agricultural microbial feed additive. The microbial feed additive is prepared by compounding three microbes, namely bacillus subtilis, clostridium butyricum and saccharomyces cerevisiae according to a certain ratio, wherein bacillus subtilis and clostridium butyricum adopt liquid fermentation and saccharomyces cerevisiae adopts liquid-solid fermentation. As for solid culture, solid fermentation of a culture medium prepared by using lucid ganoderma cultural residue, wheat bran and the like as raw materials is carried out. The microbial feed additive is used for livestock breeding, can effectively raise immunologic functions such as disease resistance, disease prevention and the like of piglets and chicks and reduce death rate, and can be used in laying hens to enhance egg yield and improve egg quality. The microbial feed additive is of great significance for promoting sustainable development of poultry breeding industry and guaranteeing product quality and safety.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff for livestock and poultry cultivation, belong to agriculture additive for microbe feedstuff field.
Background technology
In recent years, along with the raising of aquaculture intensive degree, transmission of pathogen and invasion increase the weight of gradually; Culturist, in order to reduce the incidence of disease, save aquaculture cost, adopts antibiotic to prevent and treat usually.Long-term abuse of antibiotics, cause that animal is lower for the resistance, susceptible and antibody-resistant bacterium increase, make the control of disease more difficult with treatment, and antibiotic cannot overcome intestinal microecology disorder, medicament residue, nutrition stress wait series of problems, also make animal products simultaneously, there is very large potential safety hazard in the food such as esp meat, egg, milk, causes high risks to the healthy of people.
Additive for microbe feedstuff is a class no side effects, have no drug resistance, the preparation of non-environmental-pollution, not only has to maintain intestinal microecology balance, promote growth of animal, produce digestive ferment and vitamin, improve food conversion ratio and animal feeding benefit.And partial function bacterium can produce antibacterial peptide matters, there is the effect suppressing harmful bacteria, strengthen human body immune function, reduce mortality of animals.More and more important effect is being played in replacement antibiotic, the consumption of minimizing chemical synthetic drug in livestock and poultry cultivation.Day by day become the development trend of feed addictive.
Therefore, the present invention produces composite microbial feed additive in conjunction with different beneficial microorganism exceptional function, effect and Synergistic principle, is applied to livestock and poultry cultivation, for the sustainable health development of aquaculture industry provides technical support, has broad application prospects.
Summary of the invention
The object of the present invention is to provide a kind of additive for microbe feedstuff that can promote animal growth and development, improve efficiency of feed utilization, reduce the cub poult death rate and raising egg fowl production performance.
Main technical schemes of the present invention is:
The clostridium butyricum that additive for microbe feedstuff of the present invention comprises Bacillus subtillis BSD-2 that deposit number is CGMCC No.8440, deposit number is ATCC 19398 and deposit number are the saccharomyces cerevisiae of ATCC 9763.Bacillus subtilis BSD-2 described in additive for microbe feedstuff of the present invention and clostridium butyricum adopt solution culture fermentation, and saccharomyces cerevisiae adopts the fermentation of liquid-solid two-phase culture medium, containing Ganoderma Lucidum chaff and wheat bran in solid-phase culture base.Liquid phase medium in liquid-solid two-phase culture medium described in additive for microbe feedstuff of the present invention is GPY culture medium, solid matrix in solid-phase culture base comprises the wheat bran of 70 ~ 90 weight portions and the Ganoderma Lucidum chaff of 10 ~ 30 weight portions, adds starch 3 ~ 10 weight portion, glucose 1.5 ~ 4.0 weight portion, ammonium sulfate 1.5 ~ 2.5 weight portion and potassium dihydrogen phosphate 0.6 ~ 1.8 weight portion and water 80 ~ 200 weight portion in the solid matrix of every 100 weight portions.Bacillus subtilis described in additive for microbe feedstuff of the present invention and clostridium butyricum adopt spray drying process to make pulvis, bacterium number>=1.0 × 10
10cfu/g, dried and crushed after saccharomyces cerevisiae solid fermentation completes, bacterium number>=1.0 × 10
9cfu/g, described in additive for microbe feedstuff of the present invention, three kinds of mycorhiza are according to parts by weight, by bacillus subtilis: clostridium butyricum: saccharomyces cerevisiae=(1 ~ 3): (1 ~ 3): (4 ~ 6) are composite makes composite bacteria agent capable, total bacteria count>=4.0 × 10
9cfu/g.Bacillus subtilis BSD-2>=1.0 × 10 described in additive for microbe feedstuff of the present invention
9cfu/g, clostridium butyricum>=1.0 × 10
9cfu/g, saccharomyces cerevisiae>=1.0 × 10
8cfu/g.The application of additive for microbe feedstuff of the present invention in livestock and poultry cultivation.Additive for microbe feedstuff of the present invention is by 1: in the livestock and poultry basal diet of the mass ratio interpolation of (1000-2000), total bacteria count>=5.0 × 10 after adding in feed
6cfu/g.
The culture medium of bacillus subtilis BSD-2 of the present invention consists of: peptone 10 ~ 13 g/L, starch 8 ~ 12 g/L, beef extract 5 ~ 8 g/L, yeast extract 1 ~ 3 g/L, KH
2pO
40.5 ~ 1.5 g/L, K
2hPO
40.3 ~ 1.0 g/L, MgSO
40.5 ~ 1.0 g/L, CaCO
31 ~ 10 g/L, water 1 L, pH 7.0 ± 0.2.
The culture medium of clostridium butyricum of the present invention consists of: tryptone 0.25 ~ 0.5 g/L, yeast extract 0.5 ~ 1.0 g/L, peptone 0.25 ~ 0.5 g/L, L-cysteine hydrochloride 0.3 ~ 0.5 g/L, sodium chloride 1.0 ~ 1.5 g/L, KH
2pO
41 ~ 1.5 g/L, MgSO
40.5 ~ 0.8 g/L, mass fraction is the resazurin indicator 2 of 0.1%, water 1 L, pH value 7.0 ± 0.2.
Additive for microbe feedstuff of the present invention is used in livestock and poultry cultivation.
Additive for microbe feedstuff of the present invention, effectively can improve the feed conversion rate of piglet and chick, improves the immunologic functions such as piglet chick is disease-resistant, diseases prevention, reduces the death rate.
Additive for microbe feedstuff of the present invention is used for laying hen can be improved egg production and feed conversion rate, improve egg quality.
Bacillus subtillis BSD-2(of the present invention
bacillus subtilis): Gram-positive, raw in gemma, flagellum Zhousheng, does not have parasporal crystal to be formed.Produce antibacterial peptide, have inhibitory action to Escherichia coli, staphylococcus aureus etc., livestock and poultry resistance against diseases and immunocompetence can be significantly improved.Bacterial strain deposit number CGMCC No.8440.
Clostridium butyricum of the present invention (
clostridium butyricum): shaft-like, chaining or Dan Sheng, produce gemma, Gram-positive, and strictly anaerobic grows, and starch, glucose and carbohydrate can be utilized to produce acetic acid, butyric acid etc.Also amylase, protease etc. can be produced.There is the effect of adjustment animal and bird intestines microecological balance.Bacterial strain deposit number is ATCC 19398.
Saccharomyces cerevisiae of the present invention
(Saccharomyces cerevisiae): spherical or avette, diameter 5 ~ 10 μm, gemmation.Tunning protein content and vitamin content higher, can effectively improve livestock and poultry resistance against diseases and the speed of growth etc.Bacterial strain deposit number is ATCC 9763.
Good effect of the present invention is: compared with prior art, and first, complex micro organism fungicide prepared by the present invention, has good anti-pathogenic bacteria effect, can replace antibiotic, eliminate because antibiotic adds the side effect brought.Secondly, the microorganism that the present invention adopts is compound bacteria, relative to single bacterial strain, this compound bacteria in use makes full use of the synergistic function between bacterial strain, there is stronger adaptive capacity to environment in animal and bird intestines, not only can improve animal and bird intestines micro-ecological environment, but also livestock and poultry resistance against diseases and immunity can be improved, and improve animal products output, improve product quality.3rd, adding portion Ganoderma Lucidum chaff in the solid fermentation culture medium of saccharomyces cerevisiae, agricultural wastes not only can be made to be utilized effectively, improve the viscosity of culture medium, voidage and gas porosity, and make full use of immunity and resistance against diseases that the part nutritional labeling existed in Ganoderma Lucidum chaff improves feeding livestock and poultry.The application of additive for microbe feedstuff of the present invention reduces antibiotic to the effective way of the negative effect that livestock and poultry cultivation produces, safe significant to promoting the sustainable development of poultry farming, products quality guarantee.
culture presevation mode
Bacillus subtilis BSD-2 of the present invention, Classification And Nomenclature is
bacillus subtilis, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 6th, 2013, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 10010, culture presevation number is CGMCC No. 8440.
Clostridium butyricum of the present invention, Classification And Nomenclature is
clostridium butyricum, derive from American Type Culture Collection center, bacterial strain deposit number is ATCC 19398.
Saccharomyces cerevisiae of the present invention, Classification And Nomenclature is
saccharomyces cerevisiae, derive from American Type Culture Collection center, bacterial strain deposit number is ATCC 9763.
Detailed description of the invention
In the present invention, the Bacillus subtillis BSD-2 of bacterial strain deposit number CGMCC No.8440 and bacterial strain preservation compile the clostridium butyricum of ATCC 19398 and ATCC 9763 and saccharomyces cerevisiae can obtain respectively by China General Microbiological culture presevation administrative center and American Type Culture Collection center.
The kind of the bacillus subtilis that the present invention relates to and the culture medium of clostridium butyricum can in very large range change, the culture medium cultivating bacillus subtilis, clostridium butyricum can be can be used in for various, such as, it can be LB culture medium, also can be culture medium disclosed in this invention, when adopting culture medium disclosed in this invention to cultivate, thalli growth is relatively very fast, and fermentation efficiency is higher.Described LB culture medium is conventionally known to one of skill in the art.
Saccharomyces cerevisiae of the present invention adopts liquid-solid two-phase zymotechnique, and Liquid Culture adopts GPY culture medium, and solid culture adopts the solid medium of interpolation Ganoderma Lucidum chaff disclosed by the invention to cultivate, and concrete cultural method is consistent with conventional culture methods.
The preparation method of the additive for microbe feedstuff of various embodiments of the present invention is: bacillus subtilis and clostridium butyricum adopt Liquid Culture, after forming gemma, adopts spray drying process to make pulvis, often kind of bacterium number>=1.0 × 10 respectively
10cfu/g.Dried and crushed after saccharomyces cerevisiae solid fermentation completes, bacterium number>=1.0 × 10
9cfu/g.Three kinds of bacterium are composite by a certain percentage makes, total bacteria count>=2.0 × 10
9cfu/g.
In the present invention, the cultural method of bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae is conventionally known to one of skill in the art.
Below, by embodiment, the present invention is described in more details, but not as limitation of the present invention.
embodiment 1saccharomyces cerevisiae liquid-solid two-phase ferments
Saccharomyces cerevisiae adopts liquid-solid two-phase culture process, and liquid phase medium is GPY culture medium; The culture medium that solid-phase culture adopts conventional medium and the present invention to announce respectively ferments.
Conventional solid medium is: wheat bran: cavings=80 part: 20 parts, other compositions and ratio is added in described solid medium, be 100 g by its solid matrix composition total amount, add starch 4.0 g, glucose 2.0 g, ammonium sulfate 1.0 g, potassium dihydrogen phosphate 0.5 g, pH value nature; Stir with solid matrix again after these compositions being dissolved in 150 mL water, steam sterilizing.
The solid medium that the present invention announces is: wheat bran: Ganoderma Lucidum chaff=80 part: 20 parts.Adding other compositions and ratio in described solid medium, is 100 g by its solid matrix composition total amount, adds starch 6.0 g, glucose 4.0 g, ammonium sulfate 2.0 g, potassium dihydrogen phosphate 0.8 g, pH value nature; Stir with solid matrix again after these compositions being dissolved in 180 mL water, steam sterilizing.
Respectively by the saccharomyces cerevisiae bacterial classification cultivated with fluid nutrient medium GPY in 10%(weight ratio) ratio access sterilize solid culture medium in, stir, Conventional solid fermented and cultured 2 d, shady and cool place dried and crushed is for subsequent use.
To be used for piglet and Laying chicks administering transgenic with two kinds of solid-substrate fermentation yeast, saccharomyces cerevisiae adds in livestock and poultry basal diet in the ratio of 1: 1000, yeast count>=5.0 × 10
5cfu/g.
Piglet, with the Large White 60 of 22 age in days health wean, is divided into 3 groups at random, often organizes 20.If 2 experimental group, feed respectively and add the saccharomyces cerevisiae feed of different culture media fermentation; If 1 control group, feed perfect compound feed, do not carry out the prevention and control of the methods such as injection of antibiotic agent medication, other feeding and management is undertaken by the conventional method on pig farm, daily ration and feeding and management etc. are identical, feed 30 days, observe the impact of each process on weanling pig average daily gain, feedstuff-meat ratio, diarrhea rate.Laying chicks white 240 tests in 1 age in days Beijing, are divided into 3 groups at random, often organize 80.If 2 experimental group, feed respectively and add the S. cervisiae agent feedstuff of different culture media fermentation; If 1 blank group, other feedings and managements are undertaken by chicken house conventional method, daily ration and feeding and management identical.Feed 28 days, observe the impact of each process on chick average daily gain, feedstuff-meat ratio, death rate.Concrete outcome is in table 1.
As shown in Table 1, in basal feed, add saccharomyces cerevisiae, the average daily gain of piglet and Laying chicks can be significantly improved, reduce feedstuff-meat ratio, the diarrhea rate of piglet and the death rate of chick.The saccharomyces cerevisiae of Conventional solid culture medium and culture medium of the present invention fermentation is wherein adopted to affect difference to piglet and Laying chicks average daily gain and feedstuff-meat ratio not remarkable, but the saccharomyces cerevisiae adding culture medium of the present invention fermentation, compared with conventional medium, significantly can reduce the diarrhea rate of piglet and the death rate of Laying chicks.Illustrate that the saccharomyces cerevisiae medium preparing microbial inoculum adopting the present invention to announce can significantly improve immunity and the resistance against diseases of livestock and poultry.
Table 1
Note:
diarrhea rate=(number of times of always suffering from diarrhoea/(test pig's head number × test number of days)) × 100%.
be shown in 5% level without significant difference according to rear identical lowercase alphabet with item number in table.
embodiment 2 ~ 7
Bacillus subtilis bacterium culture medium: peptone 12 g/L, starch 10 g/L, beef extract 5 g/L, yeast extract 3 g/L, KH
2pO
40.5 g/L, K
2hPO
40.5 g/L, MgSO
40.5 g/L, CaCO
33 g/L, water 1 L, pH 7.0 ± 0.2,121 DEG C of autoclaving 30 min.
Clostridium butyricum culture medium: tryptone 0.3 g/L, yeast extract 0.5 g/L, peptone 0.4 g/L, L-cysteine hydrochloride 0.3 g/L, sodium chloride 1.0 g/L, KH
2pO
41.0 g/L, MgSO
40.5 g/L, mass fraction is the resazurin indicator 2 of 0.1%, water 1 L, pH value 7.0 ± 0.2,121 DEG C of autoclaving 30 min.
Respectively by bacillus subtilis and clostridium butyricum activation, by 10% inoculum concentration, access in respective culture medium, 30 DEG C, 200 rpm shaking table shaken cultivation 2 ~ 3 d, 80% thalline stops cultivating after forming gemma.Fermentation of bacillus subtilis level>=3.0 × 10
9cfu/g, clostridium butyricum fermentation level>=2.0 × 10
9cfu/g.Obtain pulvis respectively by spraying dry, gained pulvis is containing total number of bacteria>=1.0 × 10
10cfu/mL.
Saccharomyces cerevisiae fluid nutrient medium is GPY, and solid medium is: wheat bran 90 g, Ganoderma Lucidum chaff 10 g, starch 5 g, glucose 2 g, ammonium sulfate 1.5 g, potassium dihydrogen phosphate 0.8 g, water 150 mL, stirs and evenly mixs bottling, pH value nature, 115 DEG C of autoclaving 30 min.
Saccharomyces cerevisiae adopts liquid-solid two-phase culture process, first activates saccharomyces cerevisiae bacterial classification, goes down to posterity 2 ~ 3 times, cultivates kind of a liquid with GPY solution culture fermentation, by 10% ~ 15% access liquid spawn of solid medium weight, stirs, 30 DEG C of quiescent culture 2 ~ 3 days.Fermentation by saccharomyces cerevisiae level>=1.0 × 10
9cfu/g, cloudy place dries, and pulverizes stand-by.
Three kinds of microbial solid pulvis are counted by weight, bacillus subtilis: clostridium butyricum: saccharomyces cerevisiae=(1 ~ 3): (1 ~ 3): (4 ~ 6) mix, detect packaging.Total bacteria count>=4.0 × 10
9cfu/g.In embodiment 1 ~ 6, the concrete proportioning of three kinds of bacterium is in table 2.
Table 2
experimental example 1: the application on piglet
Adopt the additive for microbe feedstuff of embodiment 2.Respectively by bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and additive for microbe feedstuff in 1: 1000 ratio add in piglet perfect compound feed, stir stand-by, total bacteria count>=5.0 × 10 in mixed feed
6cfu/g, total bacteria count>=2.0 × 10 in bacillus subtilis and clostridium butyricum
6cfu/g, S. cervisiae number>=5.0 × 10
5cfu/g.
Test the Large White piglet 100 with 22 age in days health wean, be divided into 5 groups at random, often organize 20.If 4 experimental group, feed respectively and add the feed of bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and complex microorganism; If 1 control group, feed perfect compound feed, do not carry out the prevention and control of the methods such as injection of antibiotic agent medication, other feeding and management is undertaken by the conventional method on pig farm.When the diseases such as diarrhoea occur piglet, drug therapy is carried out in the management in conjunction with pig farm, and daily ration and feeding and management etc. are completely identical with experimental group.Feed 30 days, observe and add different microorganisms microbial inoculum to the impact of weanling pig average daily gain, feedstuff-meat ratio, diarrhea rate in table 3.
Result shows, no matter adds single microorganism microbial inoculum or composite microbial feed adds agent, and the average daily gain of piglet is apparently higher than contrast, and diarrhea rate and feedstuff-meat ratio are significantly lower than contrast.Composite microbial feed additive is compared with single microorganism microbial inoculum, the experimental group of adding single microorganism is better than from the successful of average daily gain, diarrhea rate and feedstuff-meat ratio Data Representation, the application of additive for microbe feedstuff of the present invention in pig starter feed is described, diseases prevention ability and the feed conversion rate of piglet can be significantly improved.
Table 3
Table 4
experimental example 3: the application in laying hen produces
Adopt the composite microbial feed additive of embodiment 6.Respectively by bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and composite microbial feed additive in 1: in the piglet basal diet that the ratio of (1000 ~ 2000) is added, stir stand-by, total bacteria count>=5.0 × 10 in mixed feed
6cfu/g, total bacteria count>=1.0 × 10 in bacillus subtilis and clostridium butyricum
6cfu/g, S. cervisiae number>=1.0 × 10
5cfu/g.
Experiment is tested with the white laying hen in Beijing in 28 week age, totally 1000, is divided into 5 groups at random, often organizes 200.If 4 experimental group, feed respectively and add bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and complex micro organism fungicide feed; If 1 control group, does not add any material.Other feedings and managements are undertaken by chicken house conventional method, daily ration and feeding and management identical.Feed 5 weeks, observe and add different microorganisms microbial inoculum to the impact of laying rate of laying hen, feedstuff-egg ratio, death rate in table 5.
As shown in Table 4, the experimental group death rate and the feedstuff-egg ratio that add microbial bacterial agent are starkly lower than control group, laying rate is significantly higher than control group, and adds the experimental group death rate of composite bacteria agent capable and feedstuff-egg ratio is starkly lower than the process adding single microbial inoculum, and laying rate is higher than single microbial inoculum process.The application of additive for microbe feedstuff of the present invention in egg feedstuff is described, the laying rate of laying hen, resistance against diseases and feed conversion rate can be significantly improved.
Table 5
Claims (8)
1. an additive for microbe feedstuff, is characterized in that it comprises Bacillus subtillis BSD-2 that deposit number is CGMCC No.8440, deposit number is ATCC 19398 clostridium butyricum and deposit number are the saccharomyces cerevisiae of ATCC 9763.
2. a kind of additive for microbe feedstuff according to claim 1, it is characterized in that described bacillus subtilis BSD-2 and clostridium butyricum adopt solution culture fermentation, saccharomyces cerevisiae adopts the fermentation of liquid-solid two-phase culture medium, containing Ganoderma Lucidum chaff and wheat bran in solid-phase culture base.
3. a kind of additive for microbe feedstuff according to claim 2, it is characterized in that the liquid phase medium in described liquid-solid two-phase culture medium is GPY culture medium, solid matrix in solid-phase culture base comprises the wheat bran of 70 ~ 90 weight portions and the Ganoderma Lucidum chaff of 10 ~ 30 weight portions, adds starch 3 ~ 10 weight portion, glucose 1.5 ~ 4.0 weight portion, ammonium sulfate 1.5 ~ 2.5 weight portion and potassium dihydrogen phosphate 0.6 ~ 1.8 weight portion and water 80 ~ 200 weight portion in the solid matrix of every 100 weight portions.
4. a kind of additive for microbe feedstuff according to claim 1, is characterized in that described bacillus subtilis and clostridium butyricum adopt spray drying process to make pulvis, bacterium number>=1.0 × 10
10cfu/g, dried and crushed after saccharomyces cerevisiae solid fermentation completes, bacterium number>=1.0 × 10
9cfu/g.
5. a kind of additive for microbe feedstuff according to claim 4, it is characterized in that described three kinds of mycorhiza are according to parts by weight, by bacillus subtilis: clostridium butyricum: saccharomyces cerevisiae=(1 ~ 3): (1 ~ 3): (4 ~ 6) are composite makes composite bacteria agent capable, total bacteria count>=4.0 × 10
9cfu/g.
6. a kind of additive for microbe feedstuff according to claim 5, is characterized in that described bacillus subtilis BSD-2>=1.0 × 10
9cfu/g, clostridium butyricum>=1.0 × 10
9cfu/g, saccharomyces cerevisiae>=1.0 × 10
8cfu/g.
7. a kind of additive for microbe feedstuff according to claim 1, is characterized in that its application in livestock and poultry cultivation.
8. a kind of additive for microbe feedstuff according to claim 7, to it is characterized in that described composite microbial feed additive by 1: in the livestock and poultry basal diet that the mass ratio of (1000 ~ 2000) adds, total bacteria count>=5.0 × 10 after adding in feed
6cfu/g.
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| CN104605143A (en) * | 2015-03-12 | 2015-05-13 | 湖北俊宏生态牧业开发有限责任公司 | Method for preparing chicken feed by fermenting pine needles with intestinal original probiotics of laying hens |
| CN105000975A (en) * | 2015-07-06 | 2015-10-28 | 合肥福泉现代农业科技有限公司 | Dictyophora indusiata high-permeation moisture-retention medium completely prepared from cultivation waste of JUNCAO Ganoderma lucidum and preparation method thereof |
| CN105420130A (en) * | 2015-12-28 | 2016-03-23 | 湖北华扬科技发展有限公司 | Liquid-solid two-phase fermentation method for saccharomyces cerevisiae used for feed |
| CN105685474A (en) * | 2016-02-03 | 2016-06-22 | 辽宁旭辉生物科技有限公司 | Bacillus subtilis-containing feed additive and preparation method thereof |
| CN105695370A (en) * | 2016-04-13 | 2016-06-22 | 南京工业大学 | Clostridium butyricum and culture method and application thereof |
| CN106615936A (en) * | 2016-12-20 | 2017-05-10 | 柳江县素情家禽专业合作社 | Chicken feed and preparation method thereof |
| CN106721480A (en) * | 2016-12-30 | 2017-05-31 | 河南金百合生物科技股份有限公司 | Feed addictive of layer chicken and preparation method thereof |
| CN112153904A (en) * | 2018-05-15 | 2020-12-29 | 乐斯福公司 | Probiotics for avians or for ruminants |
| CN108740313A (en) * | 2018-06-07 | 2018-11-06 | 沧州新大地生物科技有限公司 | A kind of additive for microbe feedstuff |
| CN109548963A (en) * | 2019-01-02 | 2019-04-02 | 海南舜钦生物科技有限公司 | A kind of mixed type the feed for piglet additive and preparation method thereof |
| CN111317073A (en) * | 2020-03-26 | 2020-06-23 | 吉林农业大学 | Green compound feed additive for improving quality and hatchability of hen eggs |
| CN113136353A (en) * | 2021-05-21 | 2021-07-20 | 通化万赢生物科技有限公司 | Microbial preparation and application thereof |
| CN113604380A (en) * | 2021-07-15 | 2021-11-05 | 山东益昊生物科技有限公司 | Method for producing composite microbial inoculum by fermenting clostridium butyricum centrifugal waste liquid, corresponding composite microbial inoculum and application thereof |
| CN113699201A (en) * | 2021-08-25 | 2021-11-26 | 吉林省畜牧兽医科学研究院 | Method for preparing bioactive peptide |
| CN113999787A (en) * | 2021-08-30 | 2022-02-01 | 西安海吉焦生物科技研究院有限公司 | Clostridium butyricum, microbial inoculum, preparation method and application of microbial inoculum and feed |
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Application publication date: 20150311 |