CN104232592B - A kind of 451 plants of avian infectious bronchitis viruses and its application - Google Patents
A kind of 451 plants of avian infectious bronchitis viruses and its application Download PDFInfo
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- CN104232592B CN104232592B CN201410340674.6A CN201410340674A CN104232592B CN 104232592 B CN104232592 B CN 104232592B CN 201410340674 A CN201410340674 A CN 201410340674A CN 104232592 B CN104232592 B CN 104232592B
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Abstract
The present invention relates to infectious bronchitis vaccines field in veterinary biological product, particularly to a kind of 451 plants of infectious bronchitis of chicken, its deposit number is CGMCC No.8882.The 451 plants of applications in preparing infectious bronchitis of chicken live vaccine of described infectious bronchitis of chicken.451 plants of avian infectious bronchitis viruses have weaker pathogenic to chicken; and there is good immunogenicity; apply the live vaccine of its preparation safe and reliable; not only it is provided that homology counteracting toxic substances are protected; also certain cross protection can be provided for heterologous counteracting toxic substances, the M ＆ M connecing breeder flock all significantly reduces, can effectively prevent the popular of infectious bronchitis of chicken and propagate; reduce the economic loss that primary disease causes, have broad application prospects.
Description
Technical field
The present invention relates to avian infectious bronchitis viruses and technical field of vaccines, particularly to a kind of avian infectious gas
Pipe 51 plants of virus-4 of inflammation, further relates to the application of 451 plants of described avian infectious bronchitis viruses.
Background technology
Infectious bronchitis of chicken (infectious bronchitis, IB), is by infectious bronchitis virus
A kind of acute, highly infectious the viral respiratory of the chicken that (infectious bronchitis virus. IBV) causes
Disease, with tracheal sound, cough with play spray and be characterized.This disease mainly causes the weightening of chicken and the price of deed to reduce in broiler,
After IBV damages respiratory tract, also can cause insecondary bacterium infection, the chickling mortality rate causing may be up to more than 30%.Some IB
Strain can cause serious nephropathy pathological changes.IBV also can replicate in fallopian tube, after Adult Chicken infection egg production can decline 10%,
50% or more, abnormal-shape egg increasing number simultaneously, eggshell color and luster changes, and chicken infected laying rate typically can not be recovered again
To normal level, and sometimes also result in fallopian tube after Chickens Infected IB and be unable to normal development.This disease at present oneself through become to support
Chicken industry endangers one of disease of most serious.
Avian infectious bronchitis viruses（infectious bronchitis virus, IBV）Belong to coronaviridae hat
Y- coronavirus in shape Tobamovirus, are referred to as infectious bronchitis of chicken (chicken by the disease that the infection of this virus causes
Infectioubronchitis, IB), vaccine immunity prevention is to control one of maximally efficient means of this disease at present.IBV's
One of important feature is exactly that serotype is numerous, and between different serotypes, intersecting protective is relatively low or even can not protect completely.Different
Serum I BV has typical region epidemiological features, and because the restructuring between virus genomic mutation and strain makes newly
Serotype continuously emerge, lead to immunoprophylaxises increasingly to complicate.Intersecting protective is good and immunogenicity is strong therefore to excavate one plant
IBV and using its develop efficient IBV vaccine be this sick prevention and control in the urgent need to.
Content of the invention
Hand over to solve immunity between above existing domestic each serotype of infectious bronchitis of chicken commercialized vaccine
The fork problem that protection is low, immunogenicity is not strong, the invention provides one plant of chicken that cross-protection is good and immunogenicity is strong passes
451 plants of metachromia bronchitis virus（infectious bronchitis virus）.
Present invention also offers described 451 plants of neutralizing antibodies between each serotype of avian infectious bronchitis viruses
Level and in zoopery the Preliminary Applications as vaccine.
The present invention is achieved by the following measures：
A kind of 451 plants of avian infectious bronchitis viruses, its deposit number is CGMCC No.8882.
Described avian infectious bronchitis viruses titre (EID50) is 10-7.83/0.2mL.
The 451 plants of applications in preparing infectious bronchitis of chicken live vaccine of described avian infectious bronchitis viruses.
In live vaccine, viral number is 10-7.83(EID50)/0.2mL.
Beneficial effects of the present invention：451 plants of avian infectious bronchitis viruses have weaker pathogenic to chicken, and have good
Good immunogenicity, applies the live vaccine of its preparation safe and reliable, is not only provided that homology counteracting toxic substances are protected, also can be different for 821 etc.
Source counteracting toxic substances provide certain cross protection, can produce stronger immunity, the M ＆ M connecing breeder flock is equal after immunity
Significantly reduce, its immune effect reaches or better than existing commercialized vaccine on market, possesses competing with similar products at home and abroad
The advantage striven, can effectively prevent the popular of infectious bronchitis and propagate, reduce the economic loss that primary disease causes, have
Wide application prospect.
Bacterial strain preservation information
The preservation time：On May 8th, 2014,
Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Deposit number：CGMCC No. 8882,
Depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica,
Classification And Nomenclature：Avian infectious bronchitis viruses.
Specific embodiment
In order to be better understood from the present invention, to further illustrate with reference to specific embodiment.
Embodiment
451 plants of avian infectious bronchitis viruses are the wild poison got from the detached pathological material of disease of Shandong chicken house for 2012
Strain, through SPF Embryo Gallus domesticus Attenuation, obtains after passing for 100 generations.IBV is rounded indefinite to polygon, and virus has cyst membrane, directly
Footpath has bar-shaped fine prominent for 120 nm. surfaces, is about 20 nm, by shadow casting technique it is observed that spontaneous disintegrate virion institute
The nucleocapsid protein (RNP, core) discharging, the RNP being in most cases able to observe that is the pallial siphuncle of a diameter of 1-2nm,
But also can see the coiled structure of a diameter of 10-15 nm once in a while, it is commonly micro- in China Committee for Culture Collection of Microorganisms
The preserving number of Bio-Centers is CGMCC No.8882.
1.1 serum cross neutralization
Result of the test shows：451 plants of positive serums of avian infectious bronchitis viruses have certain to different separation strains
Cross-protection, illustrates that 451 plants are suitable for clinically doing vaccine use, can have one to different infectious bronchitis of chickens
Fixed protection.（It is shown in Table 1）
The neutralization test result to different IBV separation strains for the 1 451 plants of positive serums of table
Wherein N represents feminine gender, and P represents weakly positive.
1.2 safety evaluatio tests
To avian infectious bronchitis viruses, 451 plants carry out safety evaluatio, and test chicken is divided into three groups：A1, A2, A3,
Every group of 20 chickens, 451 plants of injection, 491 vaccine strains, normal saline respectively, injection volume is 4.5（EID50）.Each group case fatality rate result
It is shown in Table 2.Can see from the result of case fatality rate, 451 plants as live vaccine immunity chicken, case fatality rate be 0, illustrate safe and reliable.
The safety evaluatio result of 2 451 plants of table
1.3 animal protection power tests
1.3.1 homology virulent strain counteracting toxic substances protection is used to test
In order to check 451 plants of protections as vaccine to animal, spy has made tests below.Test chicken is divided into four groups：
B1, B2, B3, every group of 30 chickens, 451 plants of immunity, normal saline, normal saline respectively, immunizing dose 4.5 (EID50).With 451
Qiang Du street strain counteracting toxic substances B1, B2 group of the non-Attenuation of strain, work(toxic agent amount 5.0 (EID50), protective rate the results are shown in Table 3.From table
It can be seen that 451 use as vaccine, chicken can be made to be protected well.
Table 3 homology virulent strain counteracting toxic substances protection result of the test
1.3.2 tested with heterologous virulent strain counteracting toxic substances protection
Test chicken is divided into four groups：C1, C2, C3, every group of 30 chickens, 451 plants of immunity, normal saline, normal saline respectively,
Immunizing dose is 4.5 (EID50).With heterologous strong by malicious 821,395,702 strain counteracting toxic substances, work(toxic agent amount 6.0 (EID50).Protective rate
The results are shown in Table 4, table 5, table 6.
The heterologous virulent strain counteracting toxic substances protection result of the test of table 4
The heterologous virulent strain counteracting toxic substances protection result of the test of table 5
The heterologous virulent strain counteracting toxic substances protection result of the test of table 6
451 plants can be seen use as live vaccine from table, have good cross-protection.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not limited by embodiment
System, other any spirit without departing from the present invention and the change made under principle, modification, combines, substitutes, simplifying and all should be
Equivalence replacement mode, is included within protection scope of the present invention.
Claims (4)
1. a kind of 451 plants of avian infectious bronchitis viruses are it is characterised in that its deposit number is CGMCC No.8882.
2. 451 plants of avian infectious bronchitis viruses according to claim 1 are it is characterised in that its virus titer EID50For
10-7.83/0.2mL.
3. 451 plants of the avian infectious bronchitis viruses for CGMCC No.8882 for the deposit number are preparing avian infectious bronchus
Application in scorching live vaccine.
4. application according to claim 3 is it is characterised in that virus titer EID in live vaccine50For 10-7.83/0.2mL.
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Citations (6)
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| CN102286638A (en) * | 2011-07-27 | 2011-12-21 | 山东省农业科学院畜牧兽医研究所 | Method for identifying avian infectious bronchitis virus |
| CN102851257A (en) * | 2012-08-27 | 2013-01-02 | 上海启盛生物科技有限公司 | Attenuated vaccine strain for avian infectious bronchitis virus and application thereof |
| CN102994460A (en) * | 2012-12-31 | 2013-03-27 | 青岛易邦生物工程有限公司 | Infectious bronchitis virus attenuated vaccine strain |
| CN103007272A (en) * | 2012-12-31 | 2013-04-03 | 青岛易邦生物工程有限公司 | Infectious chicken bronchitis vaccine |
| CN103146654A (en) * | 2013-03-25 | 2013-06-12 | 浙江诺倍威生物技术有限公司 | Artificially weakened infectious bronchitis virus and application thereof |
| CN103497934A (en) * | 2013-10-08 | 2014-01-08 | 南京天邦生物科技有限公司 | Avian infectious bronchitis virus vaccine strain (HF2 strain) and application thereof |
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2014
- 2014-07-17 CN CN201410340674.6A patent/CN104232592B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286638A (en) * | 2011-07-27 | 2011-12-21 | 山东省农业科学院畜牧兽医研究所 | Method for identifying avian infectious bronchitis virus |
| CN102851257A (en) * | 2012-08-27 | 2013-01-02 | 上海启盛生物科技有限公司 | Attenuated vaccine strain for avian infectious bronchitis virus and application thereof |
| CN102994460A (en) * | 2012-12-31 | 2013-03-27 | 青岛易邦生物工程有限公司 | Infectious bronchitis virus attenuated vaccine strain |
| CN103007272A (en) * | 2012-12-31 | 2013-04-03 | 青岛易邦生物工程有限公司 | Infectious chicken bronchitis vaccine |
| CN103146654A (en) * | 2013-03-25 | 2013-06-12 | 浙江诺倍威生物技术有限公司 | Artificially weakened infectious bronchitis virus and application thereof |
| CN103497934A (en) * | 2013-10-08 | 2014-01-08 | 南京天邦生物科技有限公司 | Avian infectious bronchitis virus vaccine strain (HF2 strain) and application thereof |
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