CN104095692A - Method for manufacturing bovine jugular vein valved conduit - Google Patents

Abstract

The invention relates to an improved method for manufacturing a medical bovine jugular vein valved conduit. The bovine jugular vein valved conduit needs to be fixed before being implanted into human body. A fixing treatment method is improved. The method is characterized in that during fixing, a tube cavity is fully filled with fixing liquid, and the conduit is soaked into a container containing the fixing liquid after the two tail ends of blood vessel are ligated. The method has the advantages that the valved conduit can be fixed fully to better enhance the tissue strength and durability; moreover, the original shape of blood vessel can be well kept, wrinkling, twisting and knotting are avoided, convenience is brought to anastomosis in surgeries, pain of a patient and trouble of an operator are reduced, the surgical risk is lowered, and the use safety is improved.

Description

The manufacture method of bovine jugular vein valved conduit

Technical field

This patent belongs to the manufacturing technology field that utilizes biomaterial for medical purpose, relates in particular to a kind of manufacture method of bovine jugular vein valved conduit.

Background technology

Congenital heart disease sickness rate occupies the first place of birth defect.Congenital heart disease approximately has 1/3 patient to exist right ventricle or left ventricular outflow tract view in various degree to block, wherein quite a few need to be applied valved conduit or Valved allograft sheet and rebuilds right ventricular outflow and can obtain radical cure, as severe lung stricture of artery, pulmonary atresia or truncus arteriosus communis etc. in tetralogy of Fallot, double outlet of right ventricle, transposition of great artery.Conventional material has xenogenesis valved conduit, allogeneic aorta valved conduit, the homograft pipeline of synthetic, but these materials all exist limitation, calcification as easy in xenogenesis pipeline, neointimal hyperplasia; Pipeline of the same race source difficulty, implants in host and still has slight rejection, and in the time rescuing infant pathological changes, size is difficult for coupling.Bovine jugular vein possesses the advantages such as source is abundant, length is enough, is considered at present a kind of biomaterial very with research future.But the same with other foreign materials in manufacturing process, all need to process through fixing, to increase tissue intensity and durability, reduce antigenicity and immunogenicity.Tradition fixing operation be by gather pruned after for blood vessel, clear water, normal saline, balanced salt solution solution clean up, directly be put in fixative and be fixed, this method can not ensure that valved conduit is fully fixing, cannot ensure the original shape of valved conduit simultaneously, be unfavorable for coincideing of operation, can not ensure the quality of product, carry out risk to patient life zone.

Summary of the invention

This patent provides a kind of manufacture method of bovine jugular vein valved conduit for solving the technical problem existing in known technology, the method had both ensured that blood vessel was inside and outside completely fixing, had ensured again the natural shape of blood vessel, not fold, non-warping knotting, is beneficial to coincideing of operation.

The technical scheme that this patent is taked for the technical problem existing in solution known technology is:

A manufacture method for bovine jugular vein valved conduit, comprises the following steps,

A. select Niu Yuan: selecting the cows of 2～5 years old age of cattle, health, free from infection is Niu Yuan;

B. gather blood vessel: scratch cattle neck fat layer and search out jugular vein blood vessel with cutter, catching blood vessel cutter to draw to heart place along blood vessel, the blood vessel taking off proceeded to cleaning in 1 minute;

C. clean: repeatedly rinse with clear water, wash color and other pollutant off, clean blood vessel is put into the ice bucket interim storage of the normal saline that fills 4 DEG C～10 DEG C, the time of storage was controlled in 3 hours;

D. primary election, cleaning: the fat of blood vessel surface and sheath are cut off with operating scissors, by pathological changes, there is the blood vessel etc. of scar to eliminate;

E. rinsing: balanced salt solution is poured in the container that fills blood vessel, stirred gently by stirring rod, blood vessel is evenly scattered, changed one time liquid every 1 hour, change altogether balanced salt solution 6 times, then again clean with normal saline, concrete operations are identical with the cleaning part in C step;

F. fixing: first by the suture ligation of blood vessel one side, the 0.6% glutaraldehyde fixative preparing is in advance poured in tube chamber, fill rear ligation opposite side, then blood vessel is put into the container that fills 0.6% glutaraldehyde fixative and fix, temperature remains on 4-12 DEG C; After 48 hours, blood vessel is transferred in 0.3% glutaraldehyde fixative again, fixes one week, for subsequent use;

G. wrap woven dacron: be sewn into tubular with the woven dacron that width is 25mm, its internal diameter should be identical at natural relaxed state diameter with blood vessel, and be enclosed within on the position of valve of blood vessel, lower port and blood vessel top layer were around each stitching one week thereon, can not piercing blood vessel in the time sewing up, and keep the natural relaxed state of blood vessel.

This patent can also adopt following technical scheme:

Described A selects the step of Niu Yuan to select stable breeding Babalus bubalis L..

Advantage and good effect that this patent has are:

This patent has adopted after above technical scheme, reasonably utilize the natural pipeline configuration of bovine jugular vein, fixative is directly poured in tube chamber, ligation blood vessel two ends, then bovine jugular vein is put in fixative, both ensure that blood vessel is inside and outside completely fixing, ensure again the natural shape of blood vessel, not fold, non-warping knotting, is beneficial to coincideing of operation.Reduce patient suffering and operator's trouble, improve the safety of its use, reduce operation risk, and improved operation efficiency.

Detailed description of the invention

For further understanding summary of the invention, the Characteristic of this patent, hereby exemplify following examples, and be described in detail as follows:

1. select Niu Yuan: 2～5 years old age of cattle, healthy (outward appearance ox hair is thin, even, shinny), and the cows of free from infection, kind is taking stable breeding Babalus bubalis L. as good.

2. gather blood vessel: scratch cattle neck fat layer and search out jugular vein blood vessel with cutter, catch blood vessel cutter to draw to heart place along blood vessel, intercept long part as far as possible and can not injure blood vessel wall, win and in process, should avoid the material of the difficult removals in subsequent handling such as fiber to pollute blood vessel, the blood vessel taking off should proceed to cleaning in 1 minute.

3. clean: use clear water (without any visible impurity) repeatedly to rinse, wash color and other pollutant off, clean blood vessel is put into the ice bucket interim storage of the normal saline that fills 4 DEG C～10 DEG C.The ice bucket that fills blood vessel is directly taken back between processing, and the time in way should be controlled in 3 hours.

4. primary election, cleaning: the fat of blood vessel surface and sheath are cut off with operating scissors.By diameter change too greatly, the blood vessel of pathological changes, scar etc. disposes.

5. rinsing: balanced salt solution is poured in the container that fills blood vessel, stirred gently by stirring rod, blood vessel is evenly scattered.Change one time liquid every 1 hour, change altogether balanced salt solution 6 times.Then again clean with normal saline.Concrete operations are identical with the cleaning part in 3 steps

6. fixing: first by the suture ligation of blood vessel one side, the 0.6% glutaraldehyde fixative preparing is in advance poured in tube chamber, fill rear ligation opposite side, then blood vessel is put into the container that fills 0.6 glutaraldehyde fixative and fix, temperature remains on 4-12 DEG C.After 48 hours, blood vessel is transferred in 0.3% glutaraldehyde fixative again, fixes one week, for subsequent use.

7. bag woven dacron: be sewn into tubular with the woven dacron that width is 25mm, this internal diameter should be identical at natural relaxed state diameter with blood vessel, and be enclosed within on the position of valve of blood vessel, lower port and blood vessel top layer were around each stitching one week thereon, certain attention can not piercing blood vessel, and keeps the natural relaxed state of blood vessel.

Although above the preferred embodiment of this patent is described; but this patent is not limited to above-mentioned detailed description of the invention; above-mentioned detailed description of the invention is only schematic; be not restrictive; those of ordinary skill in the art is under the enlightenment of this patent; not departing from the scope situation that this patent aim and claim protects, can also make a lot of forms, within these all belong to the protection domain of this patent.

Claims (2)

1. a manufacture method for bovine jugular vein valved conduit, is characterized in that: comprises the following steps,

A. select Niu Yuan: selecting the cows of 2～5 years old age of cattle, health, free from infection is Niu Yuan;

B. gather blood vessel: scratch cattle neck fat layer and search out jugular vein blood vessel with cutter, catching blood vessel cutter to draw to heart place along blood vessel, the blood vessel taking off proceeded to cleaning in 1 minute;

C. clean: repeatedly rinse with clear water, wash color and other pollutant off, clean blood vessel is put into the ice bucket interim storage of the normal saline that fills 4 DEG C～10 DEG C, the time of storage was controlled in 3 hours;

D. primary election, cleaning: the fat of blood vessel surface and sheath are cut off with operating scissors, by pathological changes, there is the blood vessel etc. of scar to eliminate;

E. rinsing: balanced salt solution is poured in the container that fills blood vessel, stirred gently by stirring rod, blood vessel is evenly scattered, changed one time liquid every 1 hour, change altogether balanced salt solution 6 times, then again clean with normal saline, concrete operations are identical with the cleaning part in C step;

F. fixing: first by the suture ligation of blood vessel one side, the 0.6% glutaraldehyde fixative preparing is in advance poured in tube chamber, fill rear ligation opposite side, then blood vessel is put into the container that fills 0.6% glutaraldehyde fixative and fix, temperature remains on 4-12 DEG C; After 48 hours, blood vessel is transferred in 0.3% glutaraldehyde fixative again, fixes one week, for subsequent use;

G. wrap woven dacron: be sewn into tubular with the woven dacron that width is 25mm, its internal diameter should be identical at natural relaxed state diameter with blood vessel, and be enclosed within on the position of valve of blood vessel, lower port and blood vessel top layer were around each stitching one week thereon, can not piercing blood vessel in the time sewing up, and keep the natural relaxed state of blood vessel.

2. the manufacture method of bovine jugular vein valved conduit according to claim 1, is characterized in that: described A selects the step of Niu Yuan to select stable breeding Babalus bubalis L..