CN104054501B - A kind of Phoebe bournei cuttage breeding method - Google Patents
A kind of Phoebe bournei cuttage breeding method Download PDFInfo
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Abstract
The invention discloses a kind of Phoebe bournei cuttage breeding method, cultivate including maternal plant, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant management and cutting seedling management operation, it is characterized in that: superior clone seedling growth is cultivated in equipped with the breeding device of substrate mother plant for cutting, block Seedling dry doubling in maternal plant base portion and spray hormone promotion rudiment, select high-quality fringe bar through root-inducing promoter process after cuttage in cool canopy, spray mixotrophism liquid, it is thus achieved that Phoebe bournei superior clone cutting seedling.This method can keep the merit of Phoebe bournei select tree completely, improve the Phoebe bournei root system absorbability to nutrition, the Phoebe bournei branch turning out high-quality makees cutting, breeding coefficient and cuttage survival rate are the highest, with low cost, promote easily, there is provided a kind of method effective, reliable for Phoebe bournei Germ-plasma resources protection and superior clone nursery stock popularization and afforestation, there is preferable Social benefit and economic benefit.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to a kind of Phoebe bournei cuttage breeding method.
Background technology
Phoebe bournei (Phoebe bournei) is Lauraceae Phoebe aiphyllium, it is II grade of endangered plants of China, in the existing evergreen broad-leaved forest in Taishan mountain being the most sporadicly distributed in height above sea level 200～1 000 m such as the West of Hubei Province of China, Southern Zhejiang, Fujian, Jiangxi, Hunan, Guangdong, In Northern Guangxi and eastern Guizhou.Phoebe bournei is the distinctive Landscape Trees of China and excellent Precious Timber Species, but owing to wildwood is few, seed production is low, it is impossible to meet the artificial afforestration demand to nursery stock.And pass through asexual propagation, it is possible to solve the problem that seed is not enough and genetic variation and genetic differentiation is big, for Phoebe bournei resource conservation with open up new approach.Qu Fenxia (study by Phoebe bournei group culturation rapid propagating technology, forestry practical technique, 2010, (11): 7-9.) being had been achieved with successfully by the method propagation ground Lignum Aquilariae Resinatum of tissue culture, but tissue culture's working condition and technology require higher, production cost is high, at present the most not yet can spread, and Technique on Cuttage Propagation is easily mastered, working condition requires low, and general nursery just can produce.nullAt home，Thank to celebrating (Phoebe bournei softwood cutting propagation technical research such as grand grade about Phoebe bournei Technique on Cuttage Propagation research report，Hunan Forestry science and technology，2011，38 (6): 43-45.)，Lei Lingjing etc. (study by Phoebe bournei Technique on Cuttage Propagation，Fujian Forestry science and technology，2006，33 (4): 104-106.)，(the impact that Phoebe bournei epicormic branch cuttage is taken root by different factors such as Shen Zhan，Sino-South African Forestry University of Science and Technology's journal，2013，33 (1): 63-67.)，Su Cunshui (study by Phoebe bournei Technique on Cuttage Propagation，Anhui agronomy circular，2011，17 (13): 26-28.)，These researchs are all to make maternal plant with seedling，It cannot be guaranteed that keep the fine quality of elite stand completely，And not about the breeding method of maternal plant、The mining method of fringe bar and management technique to maternal plant after adopting fringe，Cuttage survival rate extremely unstable.Currently without the patent of invention about Phoebe bournei cuttage breeding method.The present invention is to select the superior clone nursery stock carrying out tissue culture's gained with the tissue of select tree to make mother plant for cutting, is planted in container, can keep the fine quality of elite stand completely, and maternal plant is managed and protected and gathers convenient and easy with fringe bar, and breeding coefficient is high, and cuttage survival rate is higher.
Summary of the invention
It is an object of the invention to the deficiency for Phoebe bournei cottage propagation, it is desirable to provide a kind of Phoebe bournei cuttage breeding method.
It is an object of the invention to be achieved through the following technical solutions:
A kind of Phoebe bournei cuttage breeding method, cultivate including maternal plant, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant management and cutting seedling management operation, it is characterized in that: superior clone seedling growth is cultivated in equipped with the breeding device of substrate mother plant for cutting, block Seedling dry doubling in maternal plant base portion and spray hormone promotion rudiment, select high-quality fringe bar through root-inducing promoter process after cuttage in cool canopy, spray mixotrophism liquid, it is thus achieved that Phoebe bournei superior clone cutting seedling, comprise the following steps:
(1) maternal plant is cultivated: by superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate, during plantation maternal plant, earthing is less than the container rim of a cup of former nursery stock, and is 30～40 cm × 50 by spacing in the rows × line-spacing～60 cm put, cultivation mother plant for cutting；
(2) cutting strunk promotees to sprout: blocks trunk in maternal plant base portion, and topdresses and spray hormone promotion rudiment；
(3) cutting selects and cuttage: gather high-quality fringe bar, after processing cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium；
(4) maternal plant management: after gathering fringe bar, topdress；
(5) cutting seedling management: use covered rearing with plastic film cutting bed, raised thin film every 5～7 days, water according to a conventional method depending on substrate dry and wet situation and spray mixotrophism liquid, after cuttage rooting is stable, removes thin film.
Above step (1) maternal plant cultivate described in its component of maternal plant substrate and parts by weight be: yellow soil or forest top soil 10 parts, wood bran or coconut palm bran 10 parts, through the farm manure of overcuring or peat soil 0.1～0.3 part.
Above step (1) maternal plant cultivate described in maternal plant cultivating container be to select mouth and a diameter of 25～30 cm at the end, a height of 40～50 control root breeding devices of cm.
Above step (2) cutting strunk promote sprout described in its method of trunk of blocking be: footpath reaches the maternal plant of 0.5～0.8 cm over the ground, blocks trunk at base portion 25～30 cm.
Above step (2) cutting strunk promote to sprout described in topdress and spray its method of hormone and be: every strain maternal plant is imposed reached, by Beijing Huimin, refined organic fertilizer 5～10 g+compound fertilizer (N:P:K=15:15:15) 5～10 g that development in science and technology center produces according to a conventional method, water permeable, and to spray mass concentration according to a conventional method be 20～50 mg.L-16-Furfurylaminopurine (6KT).
It is to gather in annual 1～March to germinate the two grades of side shoots not taking out young sprout on maternal plant trunk that above step (3) cutting selects with the high-quality fringe bar described in cuttage, side shoot is cut into according to a conventional method the cutting of a length of 10～12 cm.
Above step (3) cutting selects: yellow soil 70 parts, coconut palm bran 29 parts and Vermiculitum 1 part.
Its method of topdressing described in the management of above step (4) maternal plant is: after having gathered cutting, and every strain maternal plant then imposes and reached, by Beijing Huimin, refined organic fertilizer 5～10 g+compound fertilizer (N:P:K=15:15:15) 5～10 g that development in science and technology center produces.
Mixotrophism liquid is sprayed by being the mixotrophism liquid that 0.2～0.3 % peptide urea+0.2～0.3 % ferrous sulfate forms containing mass concentration described in the management of above step (5) cutting seedling.
The present invention has the advantage that relative to existing technology
One Phoebe bournei cuttage breeding method the most of the present invention, the superior clone nursery stock carrying out tissue culture's gained with the tissue of select tree is selected to make cuttage maternal plant, the merit of select tree can be kept completely, the cutting seedling cultivated is superior clone Seedling, and seedling cost is well below tissue culture, can be as the main method for culturing seedlings of superior clone nursery stock popularization and afforestation.
One Phoebe bournei cuttage breeding method the most of the present invention, use control root breeding device plantation maternal plant, overcome the defect that conventional vessel nursery brings root to be wound around, make maternal plant form the shortest and thick side root, improve the root system absorbability to nutrition, promote maternal plant rudiment and the growth of rudiment branch；And earthing, less than the container rim of a cup of original nursery stock, is conducive to sprouting new buds from base portion, improves breeding coefficient during owing to planting maternal plant.
One Phoebe bournei cuttage breeding method the most of the present invention, sprays 6-Furfurylaminopurine after maternal plant cutting strunk, can promote the sprouting of bud, improves breeding coefficient；Spraying peptide urea+ferrous sulfate mixed solution after cuttage, can effectively facilitate the photosynthesis of cutting and the conversion of nutrient, thus promote cuttage rooting, cuttage survival rate, at about 94 %, improves cuttage survival rate.
Detailed description of the invention
By specific examples presented below, the present invention can be well understood to further, but they are not limitation of the invention.
Embodiment
1
:
By superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate, its component of maternal plant substrate and parts by weight are: yellow soil 10 parts, coconut palm bran 10 parts, peat soil 0.2 part, and maternal plant cultivating container is to select mouth and a diameter of 30 cm at the end, the control root breeding device of a height of 40 cm.During plantation maternal plant, earthing is less than the container rim of a cup of former nursery stock, and is that 40 cm × 50 cm put by spacing in the rows × line-spacing, cultivates mother plant for cutting.Footpath reaches the maternal plant of 0.5 cm over the ground, blocks trunk at base portion 30 cm.According to a conventional method every strain maternal plant is imposed and reached, by Beijing Huimin, refined organic fertilizer 5 g+compound fertilizer (N:P:K=15:15:15) 10 g that development in science and technology center produces, water permeable, and to spray mass concentration according to a conventional method be 30 mg.L-16-Furfurylaminopurine (6KT).Gather January germinate on maternal plant trunk do not take out young sprout two grades of side shoots, side shoot is cut into according to a conventional method the cutting of a length of 10 cm.Processing after cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium, its component of cutting medium and parts by weight are: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.Use covered rearing with plastic film cutting bed, raised thin film every 6 days, regard substrate dry and wet situation and water according to a conventional method and spray by the mixotrophism liquid formed as 0.2 % peptide urea+0.3 % ferrous sulfate containing mass concentration, after cuttage rooting is stable, remove thin film.After having gathered cutting, every strain maternal plant then imposes and is reached, by Beijing Huimin, refined organic fertilizer 10 g+ compound fertilizer (N:P:K=15:15:15) 5 g that development in science and technology center produces.Average every strain maternal plant obtains cutting 10, cuttage survival rate 91.7 %.
Embodiment
2
:
By superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate, its component of maternal plant substrate and parts by weight are: forest top soil 10 parts, wood bran 10 parts, through the farm manure 0.3 part of overcuring, maternal plant cultivating container is to select mouth and a diameter of 30 cm at the end, the control root breeding device of a height of 50 cm.During plantation maternal plant, earthing is less than the container rim of a cup of former nursery stock, and is that 40 cm × 60 cm put by spacing in the rows × line-spacing, cultivates mother plant for cutting.Footpath reaches the maternal plant of 0.6 cm over the ground, blocks trunk at base portion 30 cm.According to a conventional method every strain maternal plant is imposed and reached, by Beijing Huimin, refined organic fertilizer 10 g+compound fertilizer (N:P:K=15:15:15) 8 g that development in science and technology center produces, water permeable, and to spray mass concentration according to a conventional method be 50 mg.L-16-Furfurylaminopurine (6KT).Gather February germinate on maternal plant trunk do not take out young sprout two grades of side shoots, side shoot is cut into according to a conventional method the cutting of a length of 12 cm.Processing after cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium, its component of cutting medium and parts by weight are: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.Use covered rearing with plastic film cutting bed, raised thin film every 7 days, regard substrate dry and wet situation and water according to a conventional method and spray by the mixotrophism liquid formed as 0.3 % peptide urea+0.3 % ferrous sulfate containing mass concentration, after cuttage rooting is stable, remove thin film.After having gathered cutting, every strain maternal plant then imposes and is reached, by Beijing Huimin, refined organic fertilizer 10 g+ compound fertilizer (N:P:K=15:15:15) 8 g that development in science and technology center produces.Average every strain maternal plant obtains cutting 11, cuttage survival rate 95.1 %.
Embodiment
3
:
By superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate, its component of maternal plant substrate and parts by weight are: forest top soil 10 parts, coconut palm bran 10 parts, through the farm manure 0.1 part of overcuring, maternal plant cultivating container is to select mouth and a diameter of 25 cm at the end, the control root breeding device of a height of 50 cm.During plantation maternal plant, earthing is less than the container rim of a cup of former nursery stock, and is that 30 cm × 50 cm put by spacing in the rows × line-spacing, cultivates mother plant for cutting.Footpath reaches the maternal plant of 0.8 cm over the ground, blocks trunk at base portion 30 cm.According to a conventional method every strain maternal plant is imposed and reached, by Beijing Huimin, refined organic fertilizer 10 g+compound fertilizer (N:P:K=15:15:15) 5 g that development in science and technology center produces, water permeable, and to spray mass concentration according to a conventional method be 20 mg.L-16-Furfurylaminopurine (6KT).Gather January germinate on maternal plant trunk do not take out young sprout two grades of side shoots, side shoot is cut into according to a conventional method the cutting of a length of 11 cm.Processing after cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium, its component of cutting medium and parts by weight are: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.Use covered rearing with plastic film cutting bed, raised thin film every 5 days, regard substrate dry and wet situation and water according to a conventional method and spray by the mixotrophism liquid formed as 0.3 % peptide urea+0.2 % ferrous sulfate containing mass concentration, after cuttage rooting is stable, remove thin film.After having gathered cutting, every strain maternal plant then imposes and is reached, by Beijing Huimin, refined organic fertilizer 5 g+compound fertilizer (N:P:K=15:15:15) 5 g that development in science and technology center produces.Average every strain maternal plant obtains cutting 9, cuttage survival rate 93.8 %.
Claims (2)
1. a Phoebe bournei cuttage breeding method, cultivate including maternal plant, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant management and cutting seedling management operation, it is characterized in that: superior clone seedling growth is cultivated in equipped with the breeding device of substrate mother plant for cutting, block Seedling dry doubling in maternal plant base portion and spray hormone promotion rudiment, select high-quality fringe bar through root-inducing promoter process after cuttage in cool canopy, spray mixotrophism liquid, it is thus achieved that Phoebe bournei superior clone cutting seedling, comprise the following steps:
(1) maternal plant is cultivated: by superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate, during plantation maternal plant, and earthing is less than the container rim of a cup of former nursery stock, and is 30～40 cm × 50 by spacing in the rows × line-spacing～60 cm put, cultivation mother plant for cutting；
(2) cutting strunk promotees to sprout: blocks trunk in maternal plant base portion, and topdresses and spray hormone promotion rudiment；
(3) cutting selects and cuttage: gather high-quality fringe bar, after processing cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium；
(4) maternal plant management: after gathering fringe bar, topdress；
(5) cutting seedling management: use covered rearing with plastic film cutting bed, raised thin film every 5～7 days, water according to a conventional method depending on substrate dry and wet situation and spray mixotrophism liquid, after cuttage rooting is stable, removes thin film；
Step (1) maternal plant cultivate described in maternal plant matrix components and parts by weight be: yellow soil or forest top soil 10 parts, wood bran or coconut palm bran 10 parts, through the farm manure of overcuring or peat soil 0.1～0.3 part；
Step (2) cutting strunk promote to sprout described in topdress and spray Hormonal methods and be: according to a conventional method every strain maternal plant is imposed by Beijing Huimin reach refined organic fertilizer 5～10 g that development in science and technology center produces+
Compound fertilizer 5～10 g of N:P:K=15:15:15, waters permeable, and to spray mass concentration according to a conventional method be 20～50 mg L-16-Furfurylaminopurine (6-KT)；
Step (1) maternal plant cultivate described in maternal plant cultivating container be to select mouth and a diameter of 25～30 cm at the end, a height of 40～50 control root breeding devices of cm；
Step (2) cutting strunk promote sprout described in trunk method of blocking be: footpath reaches the maternal plant of 0.5～0.8 cm over the ground, blocks trunk at base portion 25～30 cm；
It is to gather in annual 1～March to germinate the two grades of side shoots not taking out young sprout on maternal plant trunk that step (3) cutting selects with the high-quality fringe bar described in cuttage, two grades of side shoots is cut into according to a conventional method the cutting of a length of 10～12 cm；
Step (3) cutting selects: yellow soil 70 parts, coconut palm bran 29 parts and Vermiculitum 1 part；
Step (4) maternal plant management described in its method of topdressing be: after having gathered cutting, every strain maternal plant then impose by Beijing Huimin reach development in science and technology center produce refined organic fertilizer 5～10 g+
Compound fertilizer 5～10 g of N:P:K=15:15:15；
Mixotrophism liquid is sprayed by being the mixotrophism liquid that 0.2～0.3 % peptide urea+0.2～0.3 % ferrous sulfate forms containing mass concentration described in the management of step (5) cutting seedling.
null2. a Phoebe bournei cuttage breeding method，It is characterized in that: by superior clone seedling growth in equipped with in the maternal plant cultivating container of maternal plant substrate，Maternal plant matrix components and parts by weight are: forest top soil 10 parts、Coconut palm bran 10 parts、Farm manure 0.1 part through overcuring，Maternal plant cultivating container is to select mouth and a diameter of 25 cm at the end、The control root breeding device of a height of 50 cm，During plantation maternal plant, earthing is less than the container rim of a cup of former nursery stock，And be that 30 cm × 50 cm put by spacing in the rows × line-spacing，Cultivate mother plant for cutting，Footpath reaches the maternal plant of 0.8 cm over the ground，Trunk is blocked at base portion 30 cm，According to a conventional method every strain maternal plant is imposed compound fertilizer 5 g being reached the refined organic fertilizer 10 g+N:P:K=15:15:15 that development in science and technology center produces by Beijing Huimin，Water permeable，And to spray mass concentration according to a conventional method be 20 mg L-16-Furfurylaminopurine (6-KT)；Gather January and on maternal plant trunk, germinate the two grades of side shoots not taking out young sprout, two grades of side shoots are cut into according to a conventional method the cutting of a length of 11 cm, after processing cutting with root-inducing promoter, according to a conventional method by cutting cuttage to cool canopy built with in the container of cutting medium, cutting medium component and parts by weight are: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part, use covered rearing with plastic film cutting bed, thin film is raised every 5 days, regard substrate dry and wet situation water according to a conventional method and spray by the mixotrophism liquid formed as 0.3 % peptide urea+0.2 % ferrous sulfate containing mass concentration, after cuttage rooting is stable, remove thin film；After having gathered cutting, every strain maternal plant then imposes compound fertilizer 5 g being reached the refined organic fertilizer 5 g+N:P:K=15:15:15 that development in science and technology center produces by Beijing Huimin.
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