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CA3173882A1 - Proteines de liaison d'acide nucleique guidees par arn, fragments et variants actifs associes et procedes d'utilisation - Google Patents

Proteines de liaison d'acide nucleique guidees par arn, fragments et variants actifs associes et procedes d'utilisation Download PDF

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Publication number
CA3173882A1
CA3173882A1 CA3173882A CA3173882A CA3173882A1 CA 3173882 A1 CA3173882 A1 CA 3173882A1 CA 3173882 A CA3173882 A CA 3173882A CA 3173882 A CA3173882 A CA 3173882A CA 3173882 A1 CA3173882 A1 CA 3173882A1
Authority
CA
Canada
Prior art keywords
sequence
rgnabp
seq
polypeptide
sequence identity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3173882A
Other languages
English (en)
Inventor
Alexandra Briner CRAWLEY
Tedd D. Elich
Joshua SAILSBERY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lifeedit Therapeutics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA3173882A1 publication Critical patent/CA3173882A1/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des compositions et des procédés de liaison à une séquence cible d'intérêt. Les compositions trouvent une utilisation dans le clivage ou la modification d'une séquence cible d'intérêt, la visualisation d'une séquence cible d'intérêt et la modification de l'expression d'une séquence d'intérêt. Les compositions comprennent des polypeptides de protéines de liaison à l'acide nucléique guidées par ARN, des polypeptides de fusion comprenant une protéine de liaison à l'acide nucléique guidée par ARN, des ARN CRISPR, des ARN CRISPR de trans-activation, des ARN guides et des molécules d'acide nucléique codant pour ceux-ci. L'invention concerne également des vecteurs et des cellules hôtes comprenant les molécules d'acide nucléique. L'invention concerne en outre des systèmes de protéines de liaison à l'acide nucléique guidées par ARN pour lier une séquence cible d'intérêt, le système de protéines de liaison à l'acide nucléique guidées par ARN comprenant un polypeptide de protéines de liaison à l'acide nucléique guidées par ARN et un ou plusieurs ARN guides.
CA3173882A 2020-05-11 2021-05-11 Proteines de liaison d'acide nucleique guidees par arn, fragments et variants actifs associes et procedes d'utilisation Pending CA3173882A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063023009P 2020-05-11 2020-05-11
US63/023,009 2020-05-11
PCT/US2021/031794 WO2021231437A1 (fr) 2020-05-11 2021-05-11 Protéines de liaison d'acide nucléique guidées par arn, fragments et variants actifs associés et procédés d'utilisation

Publications (1)

Publication Number Publication Date
CA3173882A1 true CA3173882A1 (fr) 2021-11-18

Family

ID=76270064

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3173882A Pending CA3173882A1 (fr) 2020-05-11 2021-05-11 Proteines de liaison d'acide nucleique guidees par arn, fragments et variants actifs associes et procedes d'utilisation

Country Status (2)

Country Link
CA (1) CA3173882A1 (fr)
WO (1) WO2021231437A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024095245A2 (fr) 2022-11-04 2024-05-10 LifeEDIT Therapeutics, Inc. Désaminases d'adénine évoluées et protéines de fusion de nucléase guidée par arn avec des sites d'insertion internes et procédés d'utilisation
WO2024178397A2 (fr) 2023-02-24 2024-08-29 Elevatebio Technologies, Inc. Cellules effectrices immunitaires modifiées et méthodes d'utilisation

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
US4853331A (en) 1985-08-16 1989-08-01 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
EP0388186A1 (fr) 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company Régulation externe de l'expression de gènes
ATE225853T1 (de) 1990-04-12 2002-10-15 Syngenta Participations Ag Gewebe-spezifische promotoren
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
AU7979491A (en) 1990-05-03 1991-11-27 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
CA2051562C (fr) 1990-10-12 2003-12-02 Jewel M. Payne Isolats de bacillus thuringiensis actifs contre les dipteres
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
AU668096B2 (en) 1991-08-27 1996-04-26 Syngenta Participations Ag Proteins with insecticidal properties against homopteran insects and their use in plant protection
TW261517B (fr) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
ES2273127T3 (es) 1998-02-26 2007-05-01 Pioneer Hi-Bred International, Inc. Promotor alfa-tubulin 3-18 del maiz.
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
CN1360632A (zh) 1999-05-04 2002-07-24 孟山都技术有限公司 鞘翅目毒性多肽组合物和抗虫转基因植物
EP1218513A2 (fr) 1999-09-15 2002-07-03 Monsanto Technology LLC COMPOSITIONS D'ENDOTOXINES $g(d) DU i BACILLUS THURINGIENSIS /i ACTIVES CONTRE LES LEPIDOPTERES, ET LEURS PROCEDES D'UTILISATION
US20050183161A1 (en) 2003-10-14 2005-08-18 Athenix Corporation AXMI-010, a delta-endotoxin gene and methods for its use
US7629504B2 (en) 2003-12-22 2009-12-08 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry9 nucleic acids
PL2032598T3 (pl) 2006-06-14 2013-08-30 Athenix Corp AXMI-031, AXMI-039, AXMI-040 oraz AXMI-049, rodzina genów delta-endotoksyny oraz sposoby ich zastosowania
DK2334794T3 (en) 2008-09-15 2017-02-20 Children's Medical Center Corp MODULATION OF BCL11A FOR TREATMENT OF HEMOGLOBINOPATHIES
CN102648281B (zh) 2009-07-02 2017-04-05 阿森尼克斯公司 Axmi‑205杀虫基因和它的使用方法
US8586832B2 (en) 2009-12-21 2013-11-19 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with Lepidopteran activity
CN103154247A (zh) 2010-08-19 2013-06-12 先锋国际良种公司 对鳞翅目昆虫具有活性的新苏云金杆菌基因
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
EP3816281A1 (fr) 2012-07-11 2021-05-05 Sangamo Therapeutics, Inc. Compositions et procédés pour le traitement de maladies lysosomiale congénitale
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
AU2016334225B2 (en) * 2015-10-09 2022-11-10 Monsanto Technology Llc Novel RNA-guided nucleases and uses thereof
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
EP3426778A1 (fr) * 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
EP3494215A1 (fr) 2016-08-03 2019-06-12 President and Fellows of Harvard College Éditeurs de nucléobases d'adénosine et utilisations associées
WO2018035250A1 (fr) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Méthodes d'identification de systèmes crispr-cas de classe 2
MX2020002990A (es) * 2017-09-19 2020-07-22 Evogene Ltd Genes y aislados bacterianos para conferir resistencia a insectos.
EP3755792A4 (fr) * 2018-02-23 2021-12-08 Pioneer Hi-Bred International, Inc. Nouveaux orthologues de cas9
WO2019195379A1 (fr) * 2018-04-04 2019-10-10 Lifeedit, Inc. Procédés et compositions pour identifier de nouveaux systèmes crispr
US20220096543A1 (en) 2018-12-24 2022-03-31 Virgina Tech Intellectual Properties, Inc. Tollip deficient neutrophils and uses thereof

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