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CA2433330A1 - Amorces et procedes de detection et de discrimination d'acides nucleiques - Google Patents

Amorces et procedes de detection et de discrimination d'acides nucleiques Download PDF

Info

Publication number
CA2433330A1
CA2433330A1 CA002433330A CA2433330A CA2433330A1 CA 2433330 A1 CA2433330 A1 CA 2433330A1 CA 002433330 A CA002433330 A CA 002433330A CA 2433330 A CA2433330 A CA 2433330A CA 2433330 A1 CA2433330 A1 CA 2433330A1
Authority
CA
Canada
Prior art keywords
nucleic acid
oligonucleotide
nucleotide
acid molecule
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002433330A
Other languages
English (en)
Inventor
Irina Nazarenko
Ayoub Rashtchian
Joseph Solus
Richard M. Pires
Marlene Darfler
Gulilat Gebeyehu
Mekbib Astatke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2433330A1 publication Critical patent/CA2433330A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne de nouvelles amorces et des procédés de détection de séquences d'acides nucléiques spécifiques. Les amorces et les procédés de l'invention sont utiles dans un large éventail d'applications de biologique moléculaire et sont particulièrement utiles dans la PCR allèle-spécifique.
CA002433330A 2000-12-27 2001-12-27 Amorces et procedes de detection et de discrimination d'acides nucleiques Abandoned CA2433330A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US74814600A 2000-12-27 2000-12-27
US09/748,146 2000-12-27
US33046801P 2001-10-23 2001-10-23
US60/330,468 2001-10-23
PCT/US2001/050460 WO2002057479A2 (fr) 2000-12-27 2001-12-27 Amorces et procedes de detection et de discrimination d'acides nucleiques

Publications (1)

Publication Number Publication Date
CA2433330A1 true CA2433330A1 (fr) 2002-07-25

Family

ID=26987295

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002433330A Abandoned CA2433330A1 (fr) 2000-12-27 2001-12-27 Amorces et procedes de detection et de discrimination d'acides nucleiques

Country Status (5)

Country Link
EP (1) EP1353936A4 (fr)
JP (1) JP2004532615A (fr)
CA (1) CA2433330A1 (fr)
NZ (1) NZ527128A (fr)
WO (1) WO2002057479A2 (fr)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
EP1537240A2 (fr) * 2002-09-06 2005-06-08 Johns Hopkins Singapore Pte Ltd Detection et quantification specifiques des brins
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
DK1735459T3 (da) * 2004-04-07 2012-05-29 Exiqon As Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN1280422C (zh) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
US7517977B2 (en) * 2004-10-18 2009-04-14 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
CA2595716A1 (fr) * 2005-01-25 2006-08-03 Rosetta Inpharmatics Llc Procedes pour quantifier de petites molecules d'arn
AU2007314516A1 (en) 2006-09-28 2008-05-08 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
GB0806676D0 (en) 2008-04-12 2008-05-14 Environment Agency The Environmental monitoring
EP2133434A1 (fr) * 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Procédé pour la détection d'un acide nucléique cible
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
WO2010147848A2 (fr) * 2009-06-15 2010-12-23 Rd Biosciences, Inc. Coffrets et procédés pour une amplification et une détection sélective de cibles d'acide nucléique
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
JP5851496B2 (ja) * 2010-06-14 2016-02-03 ナショナル ユニヴァーシティー オブ シンガポール 修飾ステムループオリゴヌクレオチドが仲介する逆転写および塩基間隔が制限された定量的ｐｃｒ
BR112013004044A2 (pt) 2010-08-13 2016-07-05 Envirologix Inc composições e métodos para quantificação de uma sequência de ácido nucleico em uma amostra.
EP2705159B1 (fr) * 2011-05-06 2018-03-21 Qiagen GmbH Procédés de séquençage, d'amplification et de détection d'acides nucléiques comprenant une amorce marquée en interne
UA125112C2 (uk) * 2012-04-09 2022-01-12 Енвіролоджикс Інк. Спосіб ампліфікації полінуклеотиду
WO2015077427A1 (fr) 2013-11-20 2015-05-28 The University Of Akron Chromophores de pyrrole-bf2 hautement fluorescents
US9273078B2 (en) 2013-12-05 2016-03-01 The University Of Akron Half-phthalocyanine-like chelates and synthesis thereof
CA2946737A1 (fr) 2014-04-22 2015-10-29 Envirologix, Inc. Compositions et procedes permettant d'ameliorer et/ou de predire l'amplification d'adn
CN107208146B (zh) * 2014-11-20 2022-01-18 安普里怀斯公司 用于核酸扩增的组合物和方法
WO2023129167A1 (fr) * 2021-12-30 2023-07-06 Cue Health Inc. Compositions et procédés d'amplification isotherme d'acides nucléiques

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69233391D1 (de) * 1991-11-07 2004-09-02 Nanotronics Inc Hybridisierung von mit Chromophoren und Fluorophoren konjugierten Polynukleotiden zur Erzeugung eines Donor-Donor Energietransfersystems
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
EP0930370B1 (fr) * 1997-12-15 2007-08-08 CSL Behring GmbH Amorce marquée approprié pour la détéction des acides nucléiques
US6379888B1 (en) * 1999-09-27 2002-04-30 Becton, Dickinson And Company Universal probes and methods for detection of nucleic acids

Also Published As

Publication number Publication date
EP1353936A2 (fr) 2003-10-22
EP1353936A4 (fr) 2005-05-18
NZ527128A (en) 2005-11-25
JP2004532615A (ja) 2004-10-28
WO2002057479A3 (fr) 2003-08-14
WO2002057479A2 (fr) 2002-07-25

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Legal Events

Date Code Title Description
FZDE Discontinued