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CA2478722A1 - Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype - Google Patents

Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype Download PDF

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Publication number
CA2478722A1
CA2478722A1 CA002478722A CA2478722A CA2478722A1 CA 2478722 A1 CA2478722 A1 CA 2478722A1 CA 002478722 A CA002478722 A CA 002478722A CA 2478722 A CA2478722 A CA 2478722A CA 2478722 A1 CA2478722 A1 CA 2478722A1
Authority
CA
Canada
Prior art keywords
fragments
nucleic acid
restriction
immobilized
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002478722A
Other languages
English (en)
Inventor
Pascal Mayer
Ilia Leviev
Magne Osteras
Laurent Farinelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Solexa Ltd Great Britain
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0205153A external-priority patent/GB0205153D0/en
Application filed by Solexa Ltd Great Britain, Lynx Therapeutics Inc filed Critical Solexa Ltd Great Britain
Publication of CA2478722A1 publication Critical patent/CA2478722A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne des procédés permettant de déterminer des variations de séquence à l'échelle du génome associées au phénotype d'une espèce, sans émettre d'hypothèses. Selon les procédés de l'invention, un ensemble de fragments de restriction pour chaque sous-population d'individus qui présentent ledit phénotype est généré par digestion d'acides nucléiques provenant d'un individu, au moyen d'une ou de plusieurs enzymes de restriction. Un ensemble de marques de séquence de restriction pour l'individu est ensuite déterminé à partir dudit ensemble de fragments de restriction. Lesdites marques de séquence de restriction pour la sous-population d'organismes sont comparées et regroupées en un ou plusieurs groupes, comprenant chacun des marques de séquence de restriction qui comportent des séquences homologues. Le ou les groupes obtenus de marques de séquence de restriction identifient les variations de séquence associées audit phénotype. Lesdits procédés de l'invention peuvent être utilisés, par exemple, pour l'analyse de grands nombres de variants de séquence dans de nombreux échantillons de patients afin d'identifier des facteurs de risque génétique légèrement perceptible.
CA002478722A 2002-03-05 2003-03-05 Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype Abandoned CA2478722A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36202302P 2002-03-05 2002-03-05
GB0205153A GB0205153D0 (en) 2002-03-05 2002-03-05 Methods for detecting genome-wide sequence variations associated with a phenotype
GB0205153.0 2002-03-05
US60/362,023 2002-03-05
PCT/GB2003/000941 WO2003074734A2 (fr) 2002-03-05 2003-03-05 Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype

Publications (1)

Publication Number Publication Date
CA2478722A1 true CA2478722A1 (fr) 2003-09-12

Family

ID=27790185

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002478722A Abandoned CA2478722A1 (fr) 2002-03-05 2003-03-05 Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype

Country Status (6)

Country Link
EP (1) EP1483404A2 (fr)
JP (1) JP2005518811A (fr)
KR (1) KR20050008651A (fr)
AU (1) AU2003208480A1 (fr)
CA (1) CA2478722A1 (fr)
WO (1) WO2003074734A2 (fr)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1682680B2 (fr) * 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Procedes de production d'une etiquette appariee a partir d'une sequence d'acides nucleiques et methodes d'utilisation associees
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
US20050176019A1 (en) * 2004-02-10 2005-08-11 Beutel Bruce A. Method for identification of the location of mutations in whole genomes
US20080096255A1 (en) * 2004-07-02 2008-04-24 Matthias Harbers Method for Preparing Sequence Tags
CA2615323A1 (fr) 2005-06-06 2007-12-21 454 Life Sciences Corporation Sequencage d'extremites appariees
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
DE602007009634D1 (de) * 2006-01-04 2010-11-18 Si Lok Verfahren zur zuordnung von nukleinsäuren und zur identifikation fein strukturierter variationen in nukleinsäuren sowie hilfsmittel dafür
CA2641851A1 (fr) 2006-02-08 2007-08-16 Eric Hans Vermaas Procede de sequencage d'une matrice polynucleotidique
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
AU2008282862B2 (en) 2007-07-26 2014-07-31 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
WO2012044847A1 (fr) 2010-10-01 2012-04-05 Life Technologies Corporation Adaptateurs d'acides nucléiques et leurs utilisations
WO2009089384A1 (fr) 2008-01-09 2009-07-16 Life Technologies Procédé de fabrication d'une banque de marqueurs appariés pour le séquençage d'acides nucléiques
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
WO2010048337A2 (fr) 2008-10-22 2010-04-29 Illumina, Inc. Préservation d'informations liées à une méthylation d'adn génomique
CA2770389C (fr) 2009-08-25 2015-12-29 Illumina, Inc. Procedes de selection et d?amplification de polynucleotides
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
EP3646327B1 (fr) * 2017-06-27 2024-05-22 Bonnie Berger Leighton Externalisation ouverte de génome sécurisée pour études d'association à grande échelle
JP7477183B2 (ja) * 2019-04-22 2024-05-01 ポステック・リサーチ・アンド・ビジネス・ディベロップメント・ファウンデーション 新規な等温単一反応用プローブセット及びその用途
AU2021234084A1 (en) 2020-03-09 2022-01-06 Illumina, Inc. Methods for sequencing polynucleotides
CN117813396A (zh) 2021-12-16 2024-04-02 因美纳有限公司 混合聚类
AU2022413263A1 (en) 2021-12-17 2024-01-18 Illumina, Inc. Orthogonal hybridization
WO2023175037A2 (fr) 2022-03-15 2023-09-21 Illumina, Inc. Séquençage simultané de brins de complément avant et inverse sur des polynucléotides séparés pour la détection de méthylation
EP4341435A1 (fr) 2022-03-15 2024-03-27 Illumina, Inc. Procédés de reconnaissance de bases pour nucléobases
WO2024061799A1 (fr) 2022-09-19 2024-03-28 Illumina, Inc. Polymères déformables comprenant des amorces immobilisées
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007647A1 (fr) * 1988-02-22 1989-08-24 Pioneer Hi-Bred International, Inc. Liaisons genetiques entre des genes importants du point de vue agronomique et polymorphismes de longueurs de fragments a restriction
IL103267A (en) * 1991-09-24 2004-07-25 Keygene Nv Process and kit for amplification of restriction fragment obtained from starting dna
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO2000014282A1 (fr) * 1998-09-04 2000-03-16 Lynx Therapeutics, Inc. Procede de depistage de polymorphisme genique
EP1242630B1 (fr) * 1999-12-29 2006-02-01 Keygene N.V. Methode de generation d'oligonucleotides, plus particulierement de detection de fragments de restriction amplifies obtenus au moyen d'aflp
EP2278030B1 (fr) * 2000-04-10 2017-05-24 Taxon Biosciences, Inc. Procédés pour l'étude et l'analyse génétique de populations

Also Published As

Publication number Publication date
EP1483404A2 (fr) 2004-12-08
JP2005518811A (ja) 2005-06-30
KR20050008651A (ko) 2005-01-21
AU2003208480A1 (en) 2003-09-16
WO2003074734A3 (fr) 2004-02-19
WO2003074734A2 (fr) 2003-09-12

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Legal Events

Date Code Title Description
FZDE Discontinued