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CA2377488A1 - Regulation de l'assimilation du carbone - Google Patents

Regulation de l'assimilation du carbone Download PDF

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Publication number
CA2377488A1
CA2377488A1 CA002377488A CA2377488A CA2377488A1 CA 2377488 A1 CA2377488 A1 CA 2377488A1 CA 002377488 A CA002377488 A CA 002377488A CA 2377488 A CA2377488 A CA 2377488A CA 2377488 A1 CA2377488 A1 CA 2377488A1
Authority
CA
Canada
Prior art keywords
dna fragment
polypeptide
gene
host microorganism
derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002377488A
Other languages
English (en)
Inventor
P. John Rayapati
Corey M. Crafton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Archer Daniels Midland Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2377488A1 publication Critical patent/CA2377488A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne un procédé permettant d'augmenter le rendement d'un micro-organisme par l'amélioration de l'assimilation du gaz carbonique. Plus précisément, cette invention concerne un polypeptide ayant une activité carboxylase phosphoénolpyruvate qui n'exige pas de coenzyme A acétyle pour son activation et qui est désensibilisé à la rétroinhibition par l'acide aspartique, et aux gènes codant pour ce polypeptide. Un gène codant pour une carboxylase PEP qui n'est pas régulé par acetyl-CoA ou par acide aspartique peut améliorer le flux de carbone provenant des trois intermédiaire de carbone de PEP à destination des quatre intermédiaires de carbone de OAA, former des composés dérivés de OAA, et accélérer la biosynthèse des acides aminés. Cette invention concerne aussi des molécules d'ADN de recombinaison contenant ces gènes, et une technique de production d'acides aminés utilisant cette bactérie transformée.
CA002377488A 1999-06-29 1999-06-29 Regulation de l'assimilation du carbone Abandoned CA2377488A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1999/014437 WO2001000852A1 (fr) 1999-06-29 1999-06-29 Regulation de l'assimilation du carbone

Publications (1)

Publication Number Publication Date
CA2377488A1 true CA2377488A1 (fr) 2001-01-04

Family

ID=22273055

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002377488A Abandoned CA2377488A1 (fr) 1999-06-29 1999-06-29 Regulation de l'assimilation du carbone

Country Status (8)

Country Link
EP (1) EP1196611A1 (fr)
JP (1) JP2003503064A (fr)
KR (1) KR20020015708A (fr)
CN (1) CN1373810A (fr)
AU (1) AU4720999A (fr)
CA (1) CA2377488A1 (fr)
MX (1) MXPA01013445A (fr)
WO (1) WO2001000852A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7235901A (en) 2000-07-06 2002-01-21 Novozymes As Method of preparing a dough or a baked product made from a dough, with addition of lipolytic enzymes
WO2012014228A1 (fr) 2010-07-28 2012-02-02 Abhishek Narain Singh Méthode pour supplanter l'activité du domaine allostérique d'une enzyme de façon à modifier sa rétroinhibition ou son activation
CN103981203B (zh) * 2013-02-07 2018-01-12 中国科学院天津工业生物技术研究所 5‑氨基乙酰丙酸高产菌株及其制备方法和应用
JP7360741B2 (ja) 2019-04-12 2023-10-13 ＧｒｅｅｎＥａｒｔｈＩｎｓｔｉｔｕｔｅ株式会社 遺伝子組換え微生物及びこれを用いた目的物質の生産方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0783714B2 (ja) * 1983-08-29 1995-09-13 味の素株式会社 発酵法によるｌ―アミノ酸の製造法
JPH0630587B2 (ja) * 1985-08-23 1994-04-27 住友化学工業株式会社 クロ−ン化されたトウモロコシのｐｐｃ遺伝子
GB2223754B (en) * 1988-09-12 1992-07-22 Degussa Dna encoding phosphoenolpyruvate carboxylase
CZ289051B6 (cs) * 1993-08-24 2001-10-17 Ajinomoto Co. Inc. Mutanta karboxylázy fosfoenolpyrohroznanu, kódový řetězec pro tuto mutantu, produkční mikroorganismus a způsob výroby aminokyseliny
ES2249767T3 (es) * 1994-03-04 2006-04-01 Ajinomoto Co., Inc. Procedimiento para producir l-lisina.
JPH0866189A (ja) * 1994-08-31 1996-03-12 Mitsubishi Chem Corp 新規ｄｎａ断片
CA2180202A1 (fr) * 1995-06-30 1996-12-31 Mika Moriya Methode d'amplification d'un gene grace a un transposon artificiel
JP4075087B2 (ja) * 1996-12-05 2008-04-16 味の素株式会社 Ｌ−リジンの製造法
AR015762A1 (es) * 1998-04-13 2001-05-16 Univ Georgia Una celula manipulada por ingenieria metabolica; un metodo para producir dicha celula; un metodo para producir una sustancia bioquimica derivada deoxalacetato y un fragmento de acido nucleico aislado de p. fluorescens

Also Published As

Publication number Publication date
AU4720999A (en) 2001-01-31
MXPA01013445A (es) 2002-07-30
CN1373810A (zh) 2002-10-09
EP1196611A1 (fr) 2002-04-17
JP2003503064A (ja) 2003-01-28
KR20020015708A (ko) 2002-02-28
WO2001000852A1 (fr) 2001-01-04

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