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CA2375071A1 - Procede pour reduire les phytates dans le tourteau de canola par une manipulation genetique impliquant le gene myo-inositol 1-phosphate synthase - Google Patents

Procede pour reduire les phytates dans le tourteau de canola par une manipulation genetique impliquant le gene myo-inositol 1-phosphate synthase Download PDF

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Publication number
CA2375071A1
CA2375071A1 CA002375071A CA2375071A CA2375071A1 CA 2375071 A1 CA2375071 A1 CA 2375071A1 CA 002375071 A CA002375071 A CA 002375071A CA 2375071 A CA2375071 A CA 2375071A CA 2375071 A1 CA2375071 A1 CA 2375071A1
Authority
CA
Canada
Prior art keywords
inositol
myo
brassica
sequence
phosphate synthase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002375071A
Other languages
English (en)
Inventor
Fawzy Georges
Atta A. Hussain
Wilfred A. Keller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2375071A1 publication Critical patent/CA2375071A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une plante, un fragment multicellulaire de ladite plante ou la semence de ladite plante transformée par une séquence de nucléotide de SEQ ID NO 1 ou un allèle ou encore un fragment de celui-ci ou un équivalent génétique de celui-ci conformément à l'abâtardissement du code génétique codant pour un peptide comprenant une activité <u>Brassica</u> myo-inositol 1-phosphate synthase, ladite plante, le fragment multicellulaire ou la semence ayant réduit l'activité myo-inositol 1-phosphate synthase lorsqu'elle est comparée à une plante non-transformée équivalente, un fragment multicellulaire ou une semence ce cette dernière, telle qu'il existe des phytates réduits dans cette plante, ce fragment multicellulaire ou cette semence. L'invention concerne également un procédé pour réduire les phytates dans <u>Brassica</u>. Ce procédé comprend la croissance d'une plante <u>Brassica</u> comprenant une séquence anti-sens myo-inositol 1-phosphate synthase et/ou une séquence de cosuppression myo-inositol 1-phosphate synthase qui permet de produire une quantité réduite de myo-inositol 1-phosphate synthase et en conséquence des phytates réduits dans ladite <u>Brassica</u>.
CA002375071A 1999-05-26 2000-05-25 Procede pour reduire les phytates dans le tourteau de canola par une manipulation genetique impliquant le gene myo-inositol 1-phosphate synthase Abandoned CA2375071A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13620499P 1999-05-26 1999-05-26
US60/136,204 1999-05-26
PCT/CA2000/000612 WO2000073473A1 (fr) 1999-05-26 2000-05-25 Procede pour reduire les phytates dans le tourteau de canola par une manipulation genetique impliquant le gene myo-inositol 1-phosphate synthase

Publications (1)

Publication Number Publication Date
CA2375071A1 true CA2375071A1 (fr) 2000-12-07

Family

ID=22471816

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002375071A Abandoned CA2375071A1 (fr) 1999-05-26 2000-05-25 Procede pour reduire les phytates dans le tourteau de canola par une manipulation genetique impliquant le gene myo-inositol 1-phosphate synthase

Country Status (4)

Country Link
EP (1) EP1181379A1 (fr)
AU (1) AU4905500A (fr)
CA (1) CA2375071A1 (fr)
WO (1) WO2000073473A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6774288B1 (en) 1997-07-07 2004-08-10 Basf Plant Science L.L.C. Animal feed with low phytic acid, oil, burdened and protein laden grain
WO2005113778A2 (fr) * 2004-05-20 2005-12-01 Pioneer Hi-Bred International, Inc. Polynucleotides de proteines associees a la multiresistance aux medicaments du mais et procedes d'utilisation associes
AR050866A1 (es) 2004-09-09 2006-11-29 Dow Agrosciences Llc Genes de inositol polifosfato 2-quinasa y usos de los mismos

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE392803B (sv) * 1974-06-20 1977-04-25 L I Gillberg Forfarande for framstellning av fytinsyrafattiga proteinisolat ur brassica- och crambefro
KR100225087B1 (ko) * 1990-03-23 1999-10-15 한스 발터라벤 피타아제의 식물내 발현
FR2751987B1 (fr) * 1996-08-01 1998-12-31 Biocem Phytases de plantes et applications biotechnologiques
AU746521B2 (en) * 1997-04-08 2002-05-02 E.I. Du Pont De Nemours And Company Soybean plant producing seeds with reduced levels of raffinose saccharides and phytic acid
ATE376592T1 (de) * 1997-07-22 2007-11-15 Pioneer Hi Bred Int Gene, welche den phytat-metabolismus kontrollieren und daraus entstehende anwendungen
AU747135B2 (en) * 1997-08-11 2002-05-09 Exseed Genetics L.L.C. Controlled germination using inducible phytate gene
JPH11187879A (ja) * 1997-12-26 1999-07-13 Japan Tobacco Inc Ｎｉｃｏｔｉａｎａ属植物由来の新規ＩＮＰＳ遺伝子
PL194895B1 (pl) * 1998-01-22 2007-07-31 Nat Research Council Sposób poprawiania żywieniowego profilu rośliny i genetycznie zmienione komórki rośliny, nasion lub komórki potomne
WO2000011177A1 (fr) * 1998-08-20 2000-03-02 Pioneer Hi-Bred International, Inc. Promoteurs preferes des graines

Also Published As

Publication number Publication date
EP1181379A1 (fr) 2002-02-27
AU4905500A (en) 2000-12-18
WO2000073473A1 (fr) 2000-12-07

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued