CA2353461A1 - Integrated portable biological detection system - Google Patents

Abstract

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by stining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the contruction of a "laboratory-on-a-chip" system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Description

INTEGRATED PORTABLE BIOLOGICAL DETECTION.SYSTEM
GOVERNMENT SUPPORT
The Government has rights in this invention pursuant to Grant No. ATP:
70NANB7H3001 awarded by the Advanced Technology Program.
FIELD OF THE INVENTION
This invention relates to devices and metha~ds for performing active, mufti-step sample preparation and molecular diagnostic analysis of biological materials.
More particularly, it relates to integrated, compact, portablE; devices for self contained sample to answer systems. Specifically, this invention relates to a device and method for performing mufti-step sample preparation ,~d assay on either two or even a single microchip.
Examples of applications for.~~this integrated system include food and/or quality monitoring, diagnosis of infectio~istdiseases and cancers, bone marrow plastesis (e.g., stem cell separation and analysis), arie'I~- Enetics-based identification of individuals for forensic purposes.
RELATED APPLICATIONS
This application claims benefit of provisional patent application no.
60/113,730, filed December 23, 1998, entitled "Fluorescent Imaging of Cells and Nucleic Acids in Bioelectronic Chips", and is a continuation-in-part of co-pending Application Serial No.
09/016,596, filed on January 30, 1998, entitled "Cha;nneI-Less Separation of Bioparticles on a Bioelectronic Chip by Dielectrophoresis", which is a continuation in part of co-pending Application Serial No. 08/709,358, filed on September 6, 1996, entitled "Apparatus and Methods for Active Biological Sample Preparation", and is a continuation-in-part of co-pending Application Serial''. No. 08/986,065,, filed on December 5, 1997, entitled "Self Addressable Self Assembling Microelectronic integrated Systems, Component Devices, Mechanisms, Methods, and Procedures for Molecular Biological Analysis and Diagnostics"; which is a continuation-in-part of Application Serial No.
08/534,454, filed September 27, 1995, entitled "Apparatus and Methods far Active Programmable Matrix Devices", now issued as U.S. Patent. No. 5,849,486, which is a continuation-in-part of Application Serial No. 08/304,657, filed September 9, 1994, WO 00/37163 PCT/US99/3109$

entitled "Automated Molecular Biological Diagnostic System'.', now issued as U.S. Patent No. 5,632,957, which is a continuation-in-part of Application Serial No.
08/271,882, filed July 7, 1994, entitled "Methods for Electronic Stringency Control for Molecular Biological Analysis and Diagnostics", now allowed, tlae specifications of which are hereby incorporated by reference as fully set forth herein.
BACKGROUND OF THE INVENTION
The following description provides a sumn-iary of information relevant to the present invention. It is not an admission that any of the information provided herein is prior art to the presently claimed invention, nor that any of the publications specifically or implicitly referenced are prior art to the invention.
Generally, analysis of biological-derived sample materials cannot occur until the sample is processed through numerous pre-analysis steps. Often, the preparation process is time consuming and laborious. For example, many immuno and molecular-biological diagnostic assays on clinical samples, such as blood or tissue cells, require separation of the molecules of interest from the crude sample by disrupting or lysing the cells to release such molecules including proteins and nucleic acids (i.e., DNA and RNA) of interest, followed by purification of such proteins and/or nucleic acids. O:niy after performing processing steps can analysis of the molecules of interest begin. Additionally, protocols used for the actual analysis of the samples require numerous more steps before useful data is obtained.
For example, charged and uncharged microparticles in solution (such as cellular material or crude extracts of protein or nucleic acids thereof) may be separated by dielectrophoresis. On a microscale, dielectrophoresis can be performed using a glass slide-based device having exposed, i.e., naked, interdigitated electrodes plated on the surface of the slide and having a flow chamber with a volume of several hundred microliters.
With such a device, cells, proteins, and nucleic acids can be separated based. on their respective dielectric properties by using separation buffers having apprapriate conductivity and an AC signal with a suitable amplitude and frequency. Such devices, however, have several problems including the nonspecific binding of both separated and unsepa~~ated cells to exposed portions of the glass surface and the electrodes. Such devices are also problematic in that the flow chamber volume (several hundred microliters) is so large that thf;rmal convection can disturb and push materials such as cells and large molecules initially attracted to and retained by the electrodes off of the electrodes. Additionally, undesired cells and molecules are not easily washed off the surface without disturbing and loosing the desired cells as such cells and molecules can interfere with fluidic flow and, hence, block the flow during wash steps.
Conventional methods to disrupt whole cells for the release of proteins and nucleic acids have employed the use of a series of high voltage DC pulses in a macrodevice, as opposed to a microchip-based device. Such conventi~anal electronic lysis techniques have several problems. For example, some commercial macro-devices use lysis conditions that do not release high molecular weight (larger than 20 lKb) nucleic acids because the high molecular weight molecules can not fit through pores created in the cell membranes using such methods. Additionally, released nucleic acids are often lost due to their non-specific binding to the surface of the lysis chamber. Such loss of material, especially when molecules of interest are in low concentration, is further compounded by the fact that the dielectro-phoretic cell separation macro-device systems are stand alone systems allowing for loss of sample in the transfer of material from one device to the other as sample preparation is I S carried forward.
Processing of the crude lysate often requires chemical reactions to remove undesired cellular components from the specifically desired ones. These reactions typically include subjecting the lysate to enzymatic reactions such as proteinase K and restriction enzymes or nucleases. Processing can also include enhancing the presence of desired molecules, particularly nucleic acids, by performing amplification reactions such as by strand displacement amplification (SDA) or polymerase chain reaction {PCR) methodologies.
These reactions are also carried out in stand-alone processes. Only after these sample prepa-ration and processing steps can assaying for data retrieval begin. Because of the numerous steps between sample collection and assay, many a technique is limited in its application by a lack of sensitivity, specificity, or reproducibility.
Attempts have been made to use dielectrophoresis to .separate and identify whole cells. For example, U.S. Patent No. 4,326,934 to Pohl discloses a method and apparatus far cell classification by continuous dielectrophoresis. With such method cells are separated by making use of both the positive and negative dielectrophoretic movement of cell particles.
Separated cells are allowed to be characterized and/or classified by viewing the characteristic deflection distance of cells moving through the positive and negative electrodes.

WO 00/37163 PCT/US99/3109$

In another example, U.S. Patent No. 5,344,535 to Belts et al. discloses a method and apparatus for the characterization of micro-organisms and other particles by dielectro-phoresis. In this system, cells are characterized by matching their signature dielectrophoretic collection rates.
In yet another example, U.S. Patent No. 5,569,:367 to Belts et al. discloses a method and apparatus for separating a mixture of cells using a pair of energized interdigitated electrodes comprised of interweaved grid-like structures arranged to obstruct flow of cells through the appratus and cause differentiation of cell types into fractions by applying a non-uniform alternating field.
In addition, other attempts have been made to combine certain processing steps ar substeps together. For example, various microrobotic systems have been proposed for preparing arrays of DNA probes on a support material. Beattie et al., disclose in "The 1992 San Diego Conference: Genetic Recognition", November, 1992, use of a microrobotic system to deposit micro-droplets containing specific DNA sequences into individual microfabricated sample wells on a glass substrate.
Various other attempts have been made to describe integrated systems formed on a single chip or substrate, wherein multiple steps of an overall sample preparation and diagnostic system would be included. A. Manz et al., in "Miniaturized Total Chemical Analysis System: A Novel Concept For Chemical Sensing", Sensors And Actuators, B 1 ( 1990), pp. 244-248, describe a 'total chemical analysis system' (TAS) that comprises a modular construction of a miniaturized TAS. In that system; sample transport, chemical reactions, chromatographic separations and detection were to be automatically carried out.
Yet another proposed integrated system by Stapleton, U.S. Patent No.

5,451,500, a system for automated detection of target nucleic acid sequences is described.
In this system multiple biological samples are individually incorporats~d into matrices containing carriers in a two-dimensional format.
Various multiple electrode systems are also disclosed which purport to perforni multiple aspects of biological sample preparation or analysis. Pace, U.S.
Patent 4,908,112, entitled "Silicon Semiconductor Wafer for Analyzing Micronic Biological Samples"
describes an analytical separation device in which a capillary-sized conduit is formed by a channel in a semiconductor device, wherein electrodes are positioned in the channel to activate motion of liquids through the conduit. Additionally, Soane et al., in U.S. Patent 5,126,022, entitled "Method and Device for Moving Molecules by the Application of a Plurality of Electrical Fields", describes a system by which materials are moved through trenches by application of electric potentials to electrodes in which selected components may be guided to various trenches filled with antigen-antibodies reactive with given charged 5 particles being moved in the medium or moved. into contact with complementary components, dyes, fluorescent tags, radiolabels, enzyme-specific tags or other types of chemicals for any number of purposes such as various transformations which are either physical or chemical in nature. Further, Clark, et al. in U.S. Patent 5,194,133, entitled "Sensor Devices", discloses a sensor device for the analysis of a sample fluid which includes a substrate having a surface in which is formed an elaongate micro-machined channel con-taining a material, such as starch, agarose, alginate, carrageenan or polyacrylamide polymer gel, for causing separation of the sample fluid as the fluid passes along the channel. The biological material may comprise, for example, a bin<iing protein, an antibody, a lectin, an enzyme, a sequence of enzymes, or a lipid.
Various devices for eluting DNA from various surfaces are known. For example, Shukla U.S. Patent 5,340,449, entitled "Apparatus for 131ectroelution"
describes a system and method for the elution of macromolecules such as proteins and nucleic acids from solid phase matrix materials such as polyacrylarnide, agarose and membranes such as PVDF
in an electric field. Materials are eluted from the solid phase into a volume defined in part by molecular weight cut-off membranes. Also, Okano, et al. in U.S. Patent 5,434,049, entitled "Separation of Polynucleotides Using Supports Having a Plurality of Electrode-Containing Cells" discloses a method for detecting a plurality of target polynucleotides in a sample, the method including the step of applying a potential to individual chambers so as to serve as electrodes to elute captured target polynucleotides, the eluted material is then available for collection.
Other devices for performing nucleic acid dial;nosis have beew designed wherein at least two reaction chambers are necessary for carryout the sample preparation and analysis such as R. Lipshutz, et al., entitled "Integrated Nucleic Acid Diagnostic Device" (U.S. Patent Na. 5,856,174) and R. Anderson, et al., entitled "Integrated Nucleic Acid Diagnostic Device", {U.S. Patent No. 5,922,591 }.
Still other achievements have been made toward partial integration of a complete sample handling system such as P. Wilding, et al., "Integrated cell isolation and PCR

analysis using silicon microfilter-chambers," Anal. ~iiochemy 257, pp. 95-100, 1998; and P.C.H. Li and D.J. Harrison, "Transport, manipulation, and reaction of biological cells on-chip using electrokinetic effects," Anal. Chem., 69, pp. 1564-1568, 1997.
Still others have attempted to integrate chemical reactions with detection such as S M.A. Burns, et al., "An integrated nanoliter DNA analysis device," Science, 282, pp. 484 487, 1998; S.C. Jacobson and J.M. Ramsey, "Integrated microdevice.for DNA
restriction fragment analysis," Anal. Chem., 68, pp. 720-723, 1'996; L.C. Waters, et al., "Microchip device for cell lysis, multiplex PCR amplification" and electrophoretic sizing," Anal.
Chem., 70, pp. 158-162, 1998; and A.T. WooIley, et al., "Functional integration of PCR
amplification and capillary electrophoresis in a microfabricated DNA analysis device,"
Anal. Chem., 68, pp. 4081-4086, 1996.
Generally, as is understandable from the forgoing examples, systems and methods have been described that do not fully provide for a completely integrated self contained sample to answer system that uses electronically active: microchips. Moreover, numerous of I S the described systems are extremely labor and time intensive requiring multiple steps and human intervention either during the process or between processes, which together are suboptimal allowing for loss of sample, contamination, and operator error.
Further, the use of multiple processing steps using multiple machines or complicated robotic systems for performing the individual processes is often prohibitive except for the largest laboratories, both in terms of the expense and physical space requir<~ments. For the reasons stated above, these techniques are limited and lacking. They are not .easily combined to form a system that can carry out a complete self contained integrated diagnostic assay, particularly assays for data retrieval for nucleic acids and protein-derived information, on a single electronically addressable microchip. Despite the long-recognized need for such an integrated system without a complicated fluidics and inadequate valve systems, no satisfactory solution has previously been proposed. There is therefore a continuing need for methods and devices which lead to improved dielectrophoretic separation oiF biological cells as well as improved biological stability of the separated cells and further a continuing need for methods and devices which improve cell preparation and analysis, and which are capable of integrating cell separation, preparation, purification, and analysiis in a single self contained system without complicated fluidics.

SUMMARY OF THE INVENTION
Accordingly, provided herein is an integrated, portable system and device for performing active, integrated mufti-step sample preparation and molecular diagnostic analysis of biological samples using a minimal number of electronically addressable microchips.
In a preferred embodiment, the system and device has a single flow cell element far carrying out the separation, preparation, and analysis of preselected eukaryotic and/or prokaryotic cells from a mixed population of cells. This single flow cell element includes therein an electronically addressable microchip having a grid of individual electrodes. The grid can contain any number of individual electrodes and be fabricated on a silicon wafer as is well understood in the electronic arts. Generally, such a grid can comprise anywhere from 4 to 25 to 100 or even 10,000 or more individual electrodes. This single flow cell embodiment contemplates that the flow cell encompassing the electronic grid is able to achieve simple fluidic manipulation of the cells for their respective separation, lysis, clean-up of cell-derived materials of interest, and analysis of.such materials. Preselected eukaryotic and prokaryotic cells may be any type (e.g., blood cells, cancer cells, stem cells, bacteria, etc.) and obtained from any source (e.g., animal, biopsy, blood, organs, forensic samples, stool, water, etc.). Specific examples of cells that can be separated from, for example, whale blood include Escherichia coli, Salmonella typhimurium, Staphylococuus epidermus, Micrococcus lysodeikti~:us, and cultured cervical carcinoma cell s.
In an alternative embodiment, the device of t:he invention may include at least one additional flow chamber for carrying out either a variety of sample preparation steps or for analysis of prepared sample. For example, in one alternative embodiment, the system comprises a first and second flow cell chambers each having an electronically addressable microchip. In this alternative embodiment, the first chamber may be used to separate and lyse cells as well as perform sample preparation steps, while the second chamber is used to analyze the sample materials directly on the second flow cell microchip.
In another alternative embodiment comprising at least two flow cells, the first flow cell has no electronically addressable grid but does have .at least a heating element attached thereto that can be used to adjust the temperature of materials within said cell.
The second flow cell is contemplated to have an electronically addressable microarray for use in performing analysis of the sample materials.
Regardless of the embodiment utilized, the aystem of the invention provides the ability to (1 ) separate eukaryotic cell types from one another as well as eukaryotic cell types from prokarotic cell types, (2) directly process the sample materials from a crude state to a more refined state, and {3) directly analyze the sample materials on the microchip grid. Such an ability is possible by the novel use of electronic biasing at one level of voltage in the form of a dielectric current: to cause dielectrophoresis of cells, followed by an increase in voltage to lyse captured cells, followed in turn by changing the manner of biasing from an alternating current m.Dde to direct current mode for the addressing of specific electrodes on the arrays of the flow cells) to cause the transport of molecules of interest for capture/hybridization to probes previously bound to the electrode array. The system of the invention further contemplates that other appropriate sample preparation reagents may be transported to and away from the flow cells) by simplified arrangement of tubing and solenoid operated vailves and pump. Additionally, in embodiments having a first flow chamber that ins without a microchip, the system contemplates the ability to directly lyse the cells in the sample and analyze materials of interest without a need for separating the cell types. In such embodiment, the flow cell has a heating element that can be used to raise the temperature for direct lysis of the cells in the sample. Following such lysis, sample preparation steps such as protease treatment or nucleic acid amplification may be performed followed by transporting the amplified species to the second flow cell containing the electronically addressable microchip.
In another embodiment, the integrated system contemplates the direct processing of cells selectively separated or lysed in the first chamber to obtain any materials of interest from such cells. Such processing is generally directed to the isolation and purification of proteins and nucleic acids from cellular contaminants.
Numerous techniques can be performed in the preparation of molecules of interest including, but not limited to, enzymatic treatment using protease K to remove proteinaceous materials from nucleic acids of interest, enzymatic treatment using; nucleases to remove nucleic acids from proteins, digestive residue adsorption, nucleic acid amplification (e.g., by PCR and SDA), in situ buffer exchange and binding of antibodies or other protein-protein binding reactions such as receptor-ligand or enzyme-substrate for binding to proteins of interest.
In another embodiment, analysis of prepared. sample materials can comprise any number of preselected hybridization formats. In a preferred format, nucleic acids of interest are hybridized selectively through an electronically directed process as is known to those skilled in the art of electronically addressable microchips. Such hybridization formats comprise binding of nucleic acids (RNA, DNA, pNA) to probes anchored to the S microarray. Other formats contemplated for use with the system include selective capture of proteins of interest such as by antibody or other protein binding probes attached to the electronic grid. These can include other protein-protein interactions such as receptor-ligand and enzyme-substrate binding.
In still other embodiments, the device contemplates elements (e.g. buffer vials, tubing, miniature solenoid valves, and at least one pump) for carrying and transporting samples, buffers, enzymes and reagents to and fron-i said flow cell{s).
Additionally, yet other embodiments are provided for detecting the analytes and molecules of interest being assayed. In a preferred embodiment, such elements include a battery operated diode laser (preferably having a wavelength of 63S nm), and a CCD camera coupled with filters and 1 S zoom lens for astronomy of the individual electrodes of the microchip grids) of the first and/or second flow cell chamber(s). Still other methods of detection are also contemplated not requiring illumination with a light emitting device such as direct electrochemical detection as is well known by those in the art of such detection as described in P. Ropp and H.Holden Thorp, Chemist~Ny ~ Biology, 1999, Vol. 6, No.9, pp.
S99-605. Each of these electronic components are further contemplated to be coordinated through a computer and appropriate programming software as is well understood by those skilled in the electronic arts.
BRIEF DESCRIPTION OF THE DRAWINGS
2S The file of this patent contains at least one drawing executed in color.
Copies of this patent with color drawings) will be provided by the Patent and Trademark Office upon request and payment of the necessary fee. The invention will be further described with reference to the accompanying drawings in which:
Figure 1 is a simplified flow chart of the system showing three basic stages of the system including sample preparation, chemical reaction and analyte detection.
Figure 2 is a schematic showing the simple overall hardware design of the system wherein there is included a flow chamber 11 with heating element 12, a miniature desalting column 13; a laser 14 with optical path 15, and a detector such as a CCD camera I7.
Figure 3 is a perspective view showing one example of an external design for the portable system of this invention. The portable system comprises a housing 18 integrated 5 with a computer and monitor 20 via a cable 19.
Figure 4 is a an internal view of the integrated system showing various components thereof such as sample and reagent vials 21a-e, heating elements 12, CCD
camera 17, laser 14, a series of pumps 46 for each reagent vial, and other features such as a focus adjustment knob 4 and area for electronic components 5.

10 Figure 5 is a cross-section diagram for a flow cell having a microchip grid. The flaw cell in total comprises a microchip grid 23 on the inner bottom surface of the flow cell chamber 29. The flow cell is covered by a quartz window 28. The underside of the microchip substrate has attached thereto a heating element 12. The flow cell has at least two inlet ports 30.
Figure 6 is a schematic showing an alternative embodiment having two flow cells.
In this embodiment, a first flow cell can comprise either a cell having an electronically addressable microchip 31 a, or not having such a microchip 31 b. In either case, flow cell 3la,b has attached thereto a heating element 12. In this embodiment, the second flow cell 32 has an electronically addressable microchip attached to a heating element 12. As with the preferred embodiment diagramed in Fig. 2, the integrated system further includes a desalting column 13, laser 14 with light path 15, and a detector 17 positioned on a movable track 33 for positioning the detector for astronomy of either flow cell.
Figure 7 is a schematic showing a two flow chamber embodiment wherein first 31a and second 32 flow cells are arranged adjacent to one another. Also depicted are flaw systems for channeling buffers, reagents, and analytes through the flow cells and desalting column of the system.
Figure 8 shows bias pattern formats that can be used to create a pattern of dielectric force for separation of eukaryotic or prokaryotic cells. In (A) is depicted a square-wall dielectric force pattern. In (B) is depicted a checkerboard force pattern.
Figures 9 and 10 show two computer representations of dielectric field distributions corresponding to either a square-walll or a checkerboard force pattern respectively.

Figure 11 shows a photograph of an electronically addressable microarray in a flow cell during cell separation. The photo specifically depicts a bacterial species being separated from whole blood using a checkerboard dielectric force pattern applied to the electrode of the flow chamber. The bacteria are the light colored concentrations at the field charge maximums directly above the electrodes of the array while the blood cells are in between the electrodes at the field charge minimum areas.
Figures 12, 13A and I3B show a eukaryotic cells (cultured HeLa cells) being separated from blood cells (Fig. 12) and after washing (Fig. 13A) and staining with propidium iodide (Fig. 13B). The mixture of the normal human white and red blood cells was pushed to the space between the electrodes where the field was minimum as reflected by the pinkish diamond-shaped spot (Fig. 12).
Figures 14-17 show a series of photographs where bacteria cells are separated from blood cells using either a square-wall or checkerboard dielectric field pattern. Figs. 14 and show such separation for square-wall and checkerboard patterns respectively.
Figs. 16 15 and 17 show separated bacteria after washing square-wall and checkerboard patterns respectively.
Figure 18 is a photo of a PAGE showing analysis of nucleic acids released from bacteria by electronic lysis. Lanes I and 6 are markers, lanes 4 and 5 are whole bacteria DNA with and without Rnase treatment respectively, and lanes 2 and 3 are supercoiled and linear plasmid pCR2.l DNA released from the bacteria.
Figures 19 and 20 are PAGE photos showing amplification of two S. enterica genes spa Q and inv A, by SDA in the flow cell of the invention. In Fig. 19, from left to right, lanes 1 and 6 are size markers, lanes 2 and :3 are negative and positive controls respectively, lane 5 is the result of an amplification o~f inv A gene specific DNA sequence amplified using the device of the invention. In Fig. 20 lane 2 is a marker, lanes 1 and 4 are positive and negative controls respectively, and lane 3 is the amplification result for the spa Q gene.
Figure 21 is a photo showing hybridization :results for spa Q gene wherein two electrode pads equipped with specific probe for spa Q gene were tested for hybridization in duplicate, a third pad specific for the gene was hybridized with amplified material from a positive tube reaction, and a fourth pad which was equipped with a nonspecific probe was tested for hybridization to amplification product produced in the flow cell.

Figure 22 is a photo showing amplified spa y gene products addressed to capture sites of electrodes of the microarray using two different biasing conditions.
In the top row is duplicate addressing to pads having capture probes specific for spa Q
sequence {lanes 3 and 4) while lanes I and 5 have nonspecific probes. In the top row, a sinusoidal wave AC
S format was used while in the bottom row a direct current (DC) format was used. The results indicate that using a DC format is slightly superior to an AC format in transporting nucleic acids to the electrodes as the DC protocol bound a higher degree of amplicon.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
Referring now to specific embodiments of the invention, a portable lab-on-a-chip system is provided wherein a sample may be processed and analyzed in at least a single flow cell comprising an electronically addressable microchip. The elements of the device are housed in a portable casing or housing which contains the sample preparation and analysis flow chamber, fluid handling systems, and illumination source (such as a battery I S operated 635 nm diode laser) and detection electronics (such as a CCD
camera coupled with a set of filters and a zoom lens for astronomy of the microchip).
External to the portable casing is a computer, such as a personal computer connected to the portable housing by a cable.
Referring now to Fig. 1, a flow chart is shown depicting three distinct sample handling stages for carrying out bioassays as contemplated using the lab-on-a-chip system of the invention. These are: (1) sample processing, (2) chemical reaction, and (3) analyte detection.
Sample handling in stage (1) processing generally comprises processing of crude biological samples (e.g., cells from blood, urine, stool, mixed cell populations, etc.) for the purpose of isolating molecules of interest such as :nucleic acids and proteins. Sample handling at the chemical reaction stage {2) involves potentially many types of molecular biological reactions for clean-up and further isolation, purification,. or amplification of molecules of interest including, but not limited to, enzymatic-based reactions such a treatment with proteases, nucleases and restriction en:ayme digestion, PCR and SDA-based nucleic acid amplification, in situ buffer exchanges, chemical labeling such as by radioisotope and fluorescence markers, and immuno-based and protein-protein reactions such as antibody-antigen, ligand-receptor, and en::yme-substrate reactions.
Sample handling at the analyte detection stage (3 ) may 'be accomplished, through numerous formats including optical detection of fluorescent (;missions, electrochemical detection, and radioisotope detection. In a preferred embodiment, this detection comprises hybridization of nucleic acids or captured proteins to the electronic grid and detection using fluorescent imaging.
In Figure 2, the system is depicted in schematic form showing a top-down or edgewise view of the basic components. In this diagram a support 10 is used to mount the flow cell I1. On the backside of the flow cell 11 mounted to the support is a heating element 12. Additionally, the flow cell may be connected via a hollow fiber to ports and to a desalting column 13 in order to purify, desalt and introduce different buffers into the flow stream. The heating element may be used to heat the flow cell for either direct heat-induced lysis of cells in the chamber or for use in stage 2 process reactions such as inhibiting enzymes and temperature cycling for nucleic acid amplification.
As will be understood by one of skill in the art, the flow cell, as well as the other electrically operated components, such as solenoid operated valves and pumps, laser and detection camera, are interconnected electronically with the computer. 20 by cabling 19 to the housing 18 far all programming and control purposes. By way of example, the power source and computer are connected to the electrodes of the flow cell 11 for programming and manipulation. In the preferred embodiment, the electronic signals that may be generated by the computer software used with the system include an AC
component, such as where the AC component is sinusoidal and used in dielectrophoresis, and such as where a DC component is square and used in hybridization. In yet a further aspect, it is preferred that a time varying electronic signal also used in the system includes an offset signal, such as where the offset signal is a DC signal.
Figure 2 further shows illumination source 14 that emits a laser beam 15.
Preferably, the illumination source 14 is a diode laser. The beam 15 is preferably incident on a beam sputter 16 that directs at least a portion of the beam 15 towards the flow cell 11 microchip for sample analysis. Radiation emitted from the microchip grid electrodes retraces the incident path, and at least a portion of that radiation is passed through the beam splitter 16 to detector 17. In the preferred embodiment, the detector 17 comprises a charged coupled device (CCD) detector. Alternatively, other detectors may be utilized.

However, for compactness, it is preferred to have a f~.etector which generally has relatively larger area coverage compared to its depth (in the direction of the emitted radiation).
Figure 3 shows a perspective view of one deaign or style for the overall portable, integrated lab-on-a-chip system. A computer 20, prf:ferably a portable notebook personal computer, includes conventional elements such as a keyboard, function keys, a monitor, and input devices, e.g., trackball, and mouse. Housing 18 serves to contain not only the preparation, reaction, and analysis platforms comprising the flow cell, but also additional equipment utilized in conjunction with computer 20 for operation of the system. For example, housing 18 may contain (Fig. 4) a power supply, waveform generator, laser, flow cell(s), CCD camera, and other electronics, fluidic systems, and reagents required for operation and control of the system.
As an example of a flow cell design, Figure 5~ shows a cross-section of such a cell.
An electronically addressable microarray 23 is mounted onto substrate 10 containing a i .0 inch square pin grid array (PGA) (068 PPG.A, 400 Square Cavity, Spectrum Semiconductor Materials, San Jose, CA). Attached to the back side of the substrate 10 is a ceramic chip heater element 12 (Dawn 505, Dawn Electronics, Carson City, Nevada).
The heating element 12 may be attached by any number of methods. In a preferred embodiment, the attachment may be achieved by placing a thermally conductive flexible adhesive 22 between the backside of the electronic microchip and the ceramic heater and then incubating at an appropriate temperature and tune to fix the adhesive.
Additionally, as is well understood in the art, these microchips are; coated with a thin permeation layer such as a hydrogel, agarose, a polymer of acryla:mide, or a sol-gel matrix or the like.
This permeation layer 25 protects the cells and molecules of interest (i.e., biomaterials) from the electrochemistry occurring at the electrode surface that would otherwise damage 25 the biomaterials (or the ability to assay them) if they were exposed directly to the electrodes.
The microchip can be then attached to a molded or machined medical grade plastic flow cell 27 so that the electronic microarray 23 makes up the inner bottom surface of a well within the flow chamber. The flow cell 27 proviides a compartment 29 for containing biological sample materials and buffers to be layered on top of the microchip.
The flow cell may be further designed to have at least 2 to 4 ports 30 for sample delivery and extraction. The flow cell 27 may further be constructed so as to accept tubing for interfacing with exterior fluidic delivery and removal systems as well as desalting.
Additionally, the flow cell compartment is covered and sealed by a fused quartz window or lid 28 for visual access to the microarray analysis sites or capture pads.
This window 28 can comprise any thickness of quartz but generally is about 0.015 inches thick.
5 The window 28 may be attached to the flow cell and the flow cell to the chip by any number of methods of which a preferred method uses, an ultraviolet (UV) curing adhesive 2G developed for optics assembly. Attachment of the flow cell to the chip may be either directly to a bare surface of the chip or to~ the permeation layer overlaying the microarray also using adhesive 26. Tubing is attached to the inlet and outlet ports using a 10 variety of methods depending on the tubing size, fittings, and the tubing base material.
In an alternative embodiment, the integrated system may use two flow cells, one for sample preparation and the second for analysis. Figure 6 shows in schematic form such an embodiment. The first flow chamber 31 is attached to a heating element 12 and may optionally have an electronically addressable microchip 31a or no such microchip 1 S 31b. In either format, i.e., with or without electronic microchip, the flow cell 31 is used to perform in a stepwise fashion cell separation and/or cell iysls, and clean-up and further preparation of molecules of interest recovered from tl'ae Iysed cells. Further preparation of molecules of interest is contemplated to include an;y number of chemical reactions and steps as stated above such as isolating specific proteins of interest, isolating nucleic acids of interest such as by treatment with proteases to remove proteinaceous material from the nucleic acids, and amplification of nucleic acids of interest such as by PCR
and SDA.
The second flow cell 32 is used for sample analysis. In a preferred embodiment both flow cells 31 and 32 are disposed on the same side of the support 10.
However, these flow cells may be disposed in alternative arrangements, including a back-to-back or a stacked configuration. Additionally, these two flow cells may be connected via a hollow fiber in order to purify, desalt and introduce different buffers into the flow stream.
Moreover, each flow cell may be designed to have independent ,temperature control elements as desired. As will be understood by one of skill in the art, each of the electronically addressable microchips within the flow cells, as well as the other electrically operated components, such as solenoid operated valves and pumps, are interconnected electronically with the computer 20 by cabling for all programming and control purposes as in the single flow cell arrangement.

Figure 6 further shows illumination source 14 that emits a laser beam 15 and operates in the same fashion as described above. A camera with zoom lens 17 for astronomy of the electronic grids of the flow cells is also incorporated on a movable track 33 so that both the first and second flow cell electronic microarray grids can be viewed.
S Figure 7 is a schematic of the alternate system showing support 10 including the flow cells for sample preparation 31a (shown as an electrode comprising flow cell) and analysis 32 (shown as a 25 electrode grid}. Each of the flow cells preferably include a plurality of ports 42 connected to tubing or hollow fiber 44 leading to electrically operated 3-way valves 45. With respect to the preparation flow cell 31a, the cell is contemplated to possess at least two ports 42, optionally at least three ports, and in the preferred embodiment four ports. With respect to the analysis flow cell 32, this cell preferably has two ports. These ports 42 and their respective tubing 44 may be utilized for various functions at different times. For example, at one time a port may be considered an input port, whereas at another time, it may comprise an output port, based on the directionality of the flow into or out of the flow cells 31 and 32 as directed by the setting of the 3-way valves 45 and directionality of pump 46. Fluids in the chambers may be mixed by "pushing" and "pulling" of the liquids in various sequences through the ports.
Such mixing may be advantageous, for example, during an amplification procedure.
Figure 7 further depicts other components of the self contained system. In one embodiment, receptacles 21 a-a provide for carrying; samples and reagents such as for washing, amplification, hybridization, and waste. In another embodiment, the support 10 has attached thereto a miniature desalting column or a hollow fiber ion-exchange unit 13 for desalting small sample volumes. Often, there is a need to perform a desalting step to lower the ionic strength of the sample prior to attempting to electronically address amplified nucleic acids to specific capture pads of the microarray. This is because reagents used in amplification steps have an ionic strength that interferes with the migration of molecules of interest under the electronic addressing protocols.
Once the amplified sample has been desalted it can be directed to the flow cell 32 for analysis on the microarray.
Whether using a single or multiple flow cell integrated system of the invention, cell separation is achieved by a dielectrophoresis technique. In such method, polarizeable particles (e.g., eukaryotic and prokaryotic cells) including those with no net charge are subject to a "dielectrophoretic" force of a non-uniform electrical field. As long as the effective polarizability of the particles is different from that of the surrounding medium, the cells will remain subject to the dielectric force. 'Che direction of migration of different cell types is determined by: ( 1 ) surface charges of the cell walls or membranes of the cell bilipid membranes, (2) the conductivity and permitivity of such cell membranes and walls, and (3) the morphologies and structural architectures of the cells.
Dielectrophoresis as practiced in the current invention has been used to selectively separate from mixed cell populations (e.g., blood cells) several types of bacteria including Escherichia toll, Salmonella typhimurium, Micrococcus lysodeikticua and Staphylococcus epidermidis as well as cancer cells such as cultured cervical carcinoma cells.
In a preferred embodiment, a flow cell useful for separating cells as practiced in this invention may be designed to include an electronically addressable microchip having at least 100 individually addressable microelectrodes capable of being biased individually l5 so that a dielectric force pattern may be generated across the microchip electronic grid.
For example, Fig. 8 shows two such force patterns. In Fig. 8A is a square-wall pattern while 8B shows a checkerboard pattern. Each of these patterns provide a unique strong and weak ionic field strength pattern that provides dielectric force sufficient to separate different cell types. The force pattern is further depicted in computer generated field patterns shown in Figs. 9 and 1D for square-wall and checkerboard respectively.
Additionally, the electrodes of the microarr;~y in the ~ flow cells) may be biased either all together as a single array for carrying out cell separation, lysis, and analysis, or may be programmed to form subarrays in any nunnber of patterns for carrying out the separation, lysis, and analysis steps. In other words, one subset may be biased to separate cells, another set may be biased to cause cell lysis, and yet another set may be used for analysis.
In an example of cell separation, Figure 11 shows the separation of Micrococcus lysodeikticus from whole blood. The bacteria are concentrated above the electrodes while the undesired blood cells, both red and white cf:lls, are dark areas in between the electrodes. The cells were made to separate using; a protocol of biasing comprising a sinusoidal signal of 10 V peak-to-peak at 10 kHz. Although this signal value was used in this instance, generally cell separation can be carried. out using sinusoidal signals wherein the Volts are between 2 and 20 peak-to-peak while frequencies are between 5 and 50 kHz.
Undesired cells are washed from the flow chamber while retaining the cells of interest. This is accomplished by maintaining an attractive bias for the cells of interest and creating a flow of wash buffer through the flow chamber. Once the cells of interest are isolated in the flow chamber they may be treated in any number of ways for further processing. In one embodiment, the cells are lysed by applying high voltage pulses of up to 450 volts with a pulse width between 10 ps and ~0 ~s as described in J.
Cheng, et al., "Preparation and hybridization analysis of DNA/RNA from E. coli on microfabricated bioelectronic chips," Nature Biotechnol., 16, pp. 54:1-546, 19,98. In another example, E.
coli cells were separated from blood cells as shown in Figures 14-.17. In Figure 14 a square-wall dielectric force pattern was used while in Fig. 15, a checkerboard pattern was used. As an example, the separation for the checkerboard pattern used a bias format of a sinusoidal signal of 10 V peak-to-peak at 10 kHz. higures 16 and 17 show how cleanly the bacterial cells were isolated following washing of the s~,uare-wall and checkerboard patterned separation.
In another example, mammalian cells are separated on the electronic grid of the flow chamber. Figures 12, 13A and 13B show separation of cervical carcinoma cells.
Fig. 12 shows initial separation wherein the cells nnove onto the electrodes of the grid where the field is at a maximum by subjecting the cells to ,a positive dielectrophoretic force. The mixture of the normal human white and red blood cells mixed with the carcinoma cells are pushed to the space between the electrodes where the field was minimum as reflected by the pinkish diamond-shaped spots. After washing, the carcinoma cells were retained by the electrodes (Fig. 13A) whereas all of the normal blood cells were washed out. Figure 13B shows the cells .after staining with propidium iodide.
As an example, the mammalian cells could be separated using the checkerboard format and applying a sinusoidal signal of 6 V peak-to-peak .at 30 kHz for 3 minutes:
J. Cheng, et al., "Isolation of Cultured Cervical Carcinoma Cells Mixed With Peripheral Blood Cells on a Bioelectronic Chip," Anal. Chem. v.70, pp2321-:?6, 1998.
Following lysis, both cellular proteins and nucleic ,acids may be retained for analysis. An example showing that nucleic acid may be retained , from lysed cells is provided in Figure 1&. There, E. coli DNA is run on a PAGE gel showing both chromosomal and plasmid DNA is retained.
Where nucleic acids are desired for analysis, further clean-up and purification may include treatment with proteases such as with Proteinase K. After such treatment, the lysate may either be analysed immediately, or amplified then analysed. If the alternate embodiment is used, the sample may be further treated in the flow 'chamber or may be exported to the second chamber for such protease treatment, amplification and analysis.
Generally, a protease treatment may be carried out in an appropriate buffer at 60°C for 15 min. Temperature control may be achieved by use of the ceramic heater element attached to the back of the microchip. Inactivation of proteinase K may be achieved by heating the sample at 95°C for 2 min. Overall, the cell separation, lysis, and protease treatment process can take between 15-25 minutes.
Where proteins are desired, nucleic acids may be removed by treating the sample with restriction enzymes and nucleases. The proteins may further be treated with various I S enzymes and partial protease treatments to release specific proteins of interest from cell membranes and other cellular components. Following the above sample processing steps additional processing may be carried out including amplification (e.g., by PCR
and SDA) and labeling with radioisotopic or fluorescent markers.
In an example of chemical reaction stage processing, specific nucleic acid sequences, (i.e., the i~vA and spa Q genes of Salmonella enterica) were amplified using SDA. SDA is preferred in this portable system as. the amplification may be performed under isothermal conditions at between 50°C and 60°C thereby eliminating high temperature denaturation cycles associated with PCR. However, in one embodiment, the portable system is capable of performing PCR amplification as the flow chamber is equipped with the aforementioned heating element that has the capability of achieving repetitive high temperature cycling.
As shown in Figures 19 and 20 SDA can be effectively performed in the device with a product yield comparable to the positive control performed in a conventional reaction tube. In this example in a volume of 50 yl, Salmonella enterica and blood cells were mixed and injected into the flow cell containing a 10,000 site electrode array. The bacterial cells were separated from the blood cells by dieiectrophoresis using a biasing protocol of a lOV peak-to-peak signal having a sinusoidal wave form with frequency of between 10 and 1 S kHz using the checkerboard pattern. The cell mixture was pumped into the flow cell at a rate of 31 ~.1 per minute until the flow chamber was filled, then the flow rate was adjusted to 12.4 ~l/min until the total SO ~l volume was passed through the 5 chamber. The cells were retained above the electrodes by influence of the positive dielectrophoretic force established by the protocol. The flow cell was then washed with water to remove cells not retained by the positive bias by pumping buffer in a reverse direction at a rate of 114 p,l/rnin for 10 minutes. The fluid content of the flow cell was then exchanged with a solution of SDA reagents for amplifying either the spa Q or inv A gene 10 sequences.
The total nucleic acid from the bacteria cells was isolated by first Iysing the cells by energizing the electrodes with pulsed direct current at 200 V having a square wave form of 10 ms duration and a total of 40 separate pulses. (Alternatively, the cells can be lysed by heating the chamber to 9S°C for five minutes). A concentrated SDA reagent and 1 S buffer stock mix was introduced into the flow cell and mixed with the denatured target nucleic acid to give the following final concentrations of SDA reaction components: S00 nM amplification primers, SO nM 'bumper' primer:>, 9.S mM magnesium acetate, 3S mM
potassium phosphate buffer pH 7.6, 80 pg/ml bovine serum albumin, and 1.4 mM
each of dATP, dGTP, TTP, and alpha-thiolated dCTP. The amplification primers were designed to 20 amplify 81 base pairs of the invA or spa Q gene and comprised the following nucleic acid sequences:
spa Q Seq. Id. No. 1 S'-accgcatcgaatgcatgtctcgggtcctggtagggttattc-3' spa Q Seq. Id. No. 2 S'-cgattccgctccagacttc;tcgggaacacacgccaagta-3' i~v A Seq. Id. No. 3 S' -accgcatcgaatgcatg;tctcgggtttcaacgtttcctgcg-3' 2S inv A Seq. Id. No. 4 S'-cgattccgctccagacttc;tcgggatcgataatgccagacg-3' Bumper primers comprised the following sequences:
spa Q Seq. Id. No. 5 S'-gcaacgattatcggc-3' spa Q Seq. Id. No. 6 S'-ccagacagtaaaaac-3' inv A Seq. Id. No. 7 S'-ttgacagaatcctca-3' inv A Seq. Id: No. 8 S'-taagacggctggta-3' The released nucleic acid was then denatured by heating the chamber to 90°C for five minutes. The chamber was then brought to 60"C and 10 p,l of ISDA reagent buffer containing enzymes was introduced into the chamber to initiate amplification (i.e., 40 units of BsoB 1 restriction endonuclease and 16 units of exo-Bst DNA polymerase).
S In the system of the invention, amplification using SDA may be carried out for between 2S and 3S minutes. In the present example, after 30 minutes of reaction a S ~1 aliquot of the reaction volume was removed for PAGF? analysis {Figs. 19 and 20).
The third stage of sample handling comprising detection of the molecules of interest is preferably carried out for proteins and nucleic acids of interest.
Such detection can use various forms of hybridization to probes previously' attached to the microarray.
For example, nucleic acids (e.g., RNA, DNA, and pNA) may be used for binding sampIe-derived nucleic acid analyte (e.g., amplified or un.amplified target nucleic acids) by hybridization. Proteins may also be made to bind to capture molecules (i.e., protien-ligand binding interactions) attached to the array. Such captu~,re molecules may comprise proteins or other molecules and the binding interaction can co:rnprise such interactions as antigen-antibody, enzyme-substrate, and receptor-ligand binding.
With respect to nucleic acids, target specica, whether amplified or not, are electronically addressed to specified capture pads of the microarray of either the single (or the secondary) flow chamber for capture by oligonracleotide probes that are anchored thereto. Preferably, the electrode array of the flow cell, (i:e:, flow cell I1, 31a or 32 depending upon the embodiment and protocol used} has at least 2S individually-addressable electrodes coated with a permeation Layer {e.g. for example, an acrylamide-based hydro-gel). The target nucleic acid is biased using a positive sinusoidal signal 2S generated using a function generator/arbitrary wave form generator (33120A, Hewlett Packard, Santa Clara, CA). The capture probe-target hybrids are then detected using fluorophore-labeled reporter probes and the CCD-based optical imaging system employed for the portable instrument shown in Figure 2. Detection with this arrangement takes approximately S min to accomplish.
Continuing with the above example, the amplification products of the spa Q and ihv A genes were available for capture and analysis. Prior to capture, the SDA
reaction solution and amplif cation products were passed through a desalting column in a volume of about 75 ul followed by exchanging the buffer 'with a 50 mM Histidine buffer. The amplification products were then addressed to specific pads of the electrodes which contained gene specific probes attached to the permeation layer overlaying the electrodes.
This was followed by washing the chamber with 200mM NaCI, 10 mM Tris pH 8.0, 1 mMEDTA, followed in turn by introduction of a bodipy 630-labeled probe oligonucleotide specific for the amplification produca at a concentration of 0.5 p.M in 200 mM NaCI, 10 mM Tris pH 8.0, 1 mM EDTA, and 100 ug/ml Calf Thymus DNA. The reporter probe solution was left in the assay cell for 10 minutes then washed with 7 - 800 p.l of 50 mM Histidine buffer. ' The chamber was then visualized using a 630 nM helium-neon laser and a computer controlled CCD camera. Figure 21 shows results of binding of the spa Q gene amplification product to individual pads of the electrode in the flow chamber. The binding shows that the amplification product produced in the flow cell annealed to the capture pads (fraction 1 and 2) satisfactorily in comparison to annealing of amplification product produced as a control in a reaction tube and then introduced' into the flow cell chamber after the fractions I and 2 had been addressed. The level of binding for the control amplified product is higher due to the higher level of amplification obtained in the control reaction. We note that very little nonspecifc binding of amplified product from the flow cell reaction bound to a capture site hawing a nonspecific capture probe.
In Figure 22, the amplified SDA products for the spa ~Q gene ,were also addressed to specific pads on the microarray using two alternate biasing formats. In one biasing protocol, the amplicons were addressed using a sinusoidally based AC format of 3.5 V
peak-to-peak at 1 kHz. This protocol also used a DC offset voltage of +2,3 V
for 5 minutes. In the other protocol, a direct current (DC) format was used comprising +2.5 V
for 2 minutes. As can be seen, the DC protocol is able to transport the molecules of interest to the capture sites with greater efficiency as the DC biased pads exhibit a greater binding signal. This result further shows the versatility of the system in that processing of sample materials (cells and molecules) may be manipulated in a wide range of adjustments that is possible using this electronic-based lab-on-a-chip system. For example, the use of an AC format though shown in the above example a;s providing less transport mobility to the specific sequence, can be performed where the voltage range is between 2.0 and 5.0 peak-to-peak, the frequency range is between 1 and 10 kHz, and the DC offset is in a range of 1-5 volts). This versatility of ranges proviides for transport, of molecules under variable buffer conditions.
Visual detection of amplification reaction products by probe hybridization may be performed by directing a battery operated diode Ia.ser having at least the capability of generating approximately 2 mWatts of laser pawer with an emission wavelength of 635 nm. The Iaser is used to excite fluorescent dye-label reporter probe (such as BODIPY-630). The wavelength of the emission filter is 67CI nm. The dichromatic mirror has a wavelength cutoff at 645nm. Alternatively, a direct electrochemical voltammetric detection system may also be used instead of light based detection as is understood by those skilled in the art.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.

SEQUENCE LISTING
<110> CHENG, JING
WU, LET
HELLER, MhCHAEL
SHELDON, ED
DIVER, JONATHAN
O'CONNELL, JAMES P.
SMOLKO, DAN
JALALI, SHILA
WILLOUGHBY, DAVID
<120> INTEGRATED PORTABLE BIOLOGICAL, DETECTION SYSTEM
<130> 238/266-PCT
<140> To be assigned <141> Filed herewith <150> 60/113,730 <151> 1998-12-23 <150> 09/016,596 <151> 1998-Ol-30 <150> 08/709,358 <151> 1996-09-06 <150> 08/986,065 <151> 1997-12-05 <150> 08/534,454 <151> 1995-09-27 <150> 08/304,657 <151> 1994-09-09 <150> 08/271,882 c151> 1994-07-07 <160> 8 <170> FastSEQ for Windows Version 3.0 <210> 1 <211> 41 <212> DNA
<213> Salmonella enterica SD-141433.1 i <400> 1 accgcatcga atgcatgtct cgggtcctgg tagggttata c <210> 2 <211> 39 <212> DNA
<213> Salmonella enterica <400> 2 cgattccgct ccagacttct cgggaacaca cgccaagta 3g <210> 3 c211> 41 <212> DNA
c213> Salmonella enterica <400> 3 accgcatcga atgcatgtct cgggtttcaa cgtttcctgc g 41 <210> 4 <211> 41 c212> DNA
<213> Salmonella enterica c400> 4 cgattccgct ccagacttct cgggatcgat aatgccagac g 41 <210> 5 <211> 15 <212> DNA
c213> Artificial Sequence <220>
<223> Synthetic nucleic acid moleculc_ <400> 5 gcaacgatta tcggc 15 <210> 6 <211> 15 <212> DNA
<213> Artificial Sequence SD-141433.1 <220>
<223> Synthetic nucleic acid molecule <400> 6 ccagacagta aaaac 15 <210> 7 <211> 15 <212> DNA
<213> Artificial Sequence <220>
<223> Synthetic nucleic acid molecule <400> 7 ttgacagaat cctca 15 <210> 8 <211> 14 <212> DNA
<213> Artificial Sequence <220>
<223> Synthetic nucleic acid molecule <400> 8 taagacggct ggta 14 SD-141433.1

Claims (139)

What is claimed is:

1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
a. an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising:
at least one input and at least one output port connected to a flow cell, a plurality of individually addressable electrodes positioned within said flow cell, and a heating element connected to said flow cell, said flow cell adapted to receive said sample via the input, said electrodes being operatively coupled to a power source adapted to electronically disrupt cell membranes within said sample, said electrodes further comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules;
b. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and c. a power source.

2. An integrated system of claim 1 wherein the sample preparation further includes the step of cell separation.

3. An integrated system of claim 2 wherein the cell separation is performed electronically.

4. An integrated system of claim 1 further comprising an illumination source operatively positioned to provide radiation to the active matrix array.

5. An integrated system of claim 4 wherein the illumination source is a laser.

6. An integrated system of claim 5 wherein the laser is a diode laser.

7. An integrated system of claim 5 wherein the system includes a beam splitter.

8. An integrated system of claim 7 wherein the beam splitter is positioned to receive the illumination and reflect at least a portion thereof to the active matrix array, and to pass at least a portion of the radiation from the active matrix array.

9. An integrated system of claim 1 wherein said plurality of individually addressable electrodes comprising the active matrix array are used as a unit for each of said cell separation, cell lysis, and sample analysis.

10. An integrated system of claim 1 wherein said plurality of individually addressable electrodes comprising the active matrix array are used independent of one another in the formation of subsets of electrodes for cell separation, cell lysis, and sample analysis.

11. An integrated system of claim 1 wherein the sample preparation includes further reaction steps after said electronic lysis.

12. An integrated system of claim 11 wherein the further reaction includes enzymatic reaction.

13. An integrated system of claim 11 wherein the further reaction includes chemical reaction.

14. An integrated system of claim 11 wherein the further reaction includes protease reactions.

15. An integrated system of claim 14 wherein the protease reaction includes protease-K reaction.

16. An integrated system of claim 1 wherein the further reaction includes an amplification reaction.

17. An integrated system of claim 16 wherein the amplification includes SDA.

18. An integrated system of claim 16 wherein the amplification includes PCR.

19. An integrated system of claim 16 wherein the amplification includes linear amplification.

20. An integrated system of claim 16 wherein the amplification includes exponential amplification.

21. An integrated system of claim 1 wherein the power source provides a time varying signal to at least certain of the electrodes.

22. An integrated system of claim 21 wherein the time varying signal includes an AC component.

23. An integrated system of claim 22 wherein the AC component is sinusoidal.

24. An integrated system of claim 21 wherein the time varying signal includes an offset signal.

25. An integrated system of claim 24 wherein the offset is a DC offset.

26. An integrated system of claim 1 further including a control system.

27. An integrated system of claim 26 wherein the control system is a computer.

28. An integrated system of claim 27 wherein the computer is a personal computer.

29. An integrated system of claim 28 wherein the personal computer is a notebook computer.

30. An integrated system of claim 1 wherein the flow cell of the electronic system includes at least one port in addition to the input and the output ports.

31. An integrated system of claim 30 wherein the flow cell of the electronic system includes at least two ports in addition to the input and the output ports.

32. An integrated system of claim 31 wherein the ports are at substantially 90 degrees to another port.

33. An integrated system of claim 30 wherein at least two of the ports are in opposition to one another.

34. An integrated system of claim 1 wherein the detector is a CCD detector.

35. An integrated system of claim l wherein the system is a closed system.

36. An integrated system of claim 1 wherein the system is a self contained system.

37. An integrated system of claim 1 wherein the system further includes a fluidic system.

38. An integrated system of claim 37 wherein the fluidic system includes a pump.

39. An integrated system of claim 37 wherein the fluidic system includes a plurality of valves.

40. An integrated system of claim 1 wherein the sample analysis includes binding of molecules to the array selected from the group consisting of hybridization and protein-ligand binding interactions.

41. An integrated system of claim 40 wherein the hybridization is DNA-DNA
hybridization.

42. An integrated system of claim 40 wherein the hybridization is DNA/RNA
hybridization.

43. An integrated system of claim 40 wherein the hybridization is pNA/nucleic acid hybridization.

44. An integrated system of claim 40 wherein the protein-ligand binding is selected from the group consisting of receptor-ligand, antigen-antibody, and enzyme-substrate binding.

45. An integrated system for the analysis of a biological sample containing eukaroytic and/or prokaryotic cells, comprising:
a. a first electronic system for sample preparation, said system further comprising:
at least one input port, a first flow cell connected to the input, a plurality of electrodes positioned within said flow cell, and at least one output port, said flow cell adapted to receive said sample via the input, said electrodes being operatively coupled to a power source adapted to electronically disrupt cell membranes within said sample;
b. a second electronic system for sample analysis, said system further comprising:
at least one input port, a second flow cell connected to the input, a plurality of electrodes positioned within said second flow cell, said electrodes comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules, and at least one output port, said input of the second electronic system being coupled to at least one output port of the first electronic system;
c. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal: and d. a power source.

46. An integrated system of claim 45 wherein the sample preparation further includes the step of cell separation.

47. An integrated system of claim 46 wherein the cell separation is performed electronically.

48. An integrated system of claim 45 further comprising an illumination source operatively positioned to provide radiation to the active matrix array.

49. An integrated system of claim 48 wherein the illumination source is a laser.

50. An integrated system of claim 49 wherein the laser is a diode laser.

51. An integrated system of claim 48 wherein the system includes a beam splitter.

52. An integrated system of claim 51 wherein the beam splitter is positioned to receive the illumination and reflect at least a portion thereof to the active matrix array, and to pass at least a portion of the radiation from the active matrix array.

53. An integrated system of claim 45 wherein said plurality of individually addressable electrodes of said first electronic system are used as a unit for each of said cell separation and cell lysis.

54. An integrated system of claim 45 wherein said plurality of individually addressable electrodes of said second electronic system are used as a unit for said analysis.

55. An integrated system of claim 45 wherein said plurality of individually addressable electrodes of said first electronic systems are used independent of one another in the formation of subsets of electrodes for cell separation and cell lysis.

56. An integrated system of claim 45 wherein said plurality of individually addressable electrodes of said second electronic system are used independent of one another in the formation of subsets of electrodes for said analysis.

57. An integrated system of claim 45 wherein the sample preparation includes further reaction steps after said electronic lysis.

58. An integrated system of claim 57 wherein the further reaction includes enzymatic reaction.

59. An integrated system of claim 57 wherein the further reaction includes chemical reaction.

60. An integrated system of claim 57 wherein the further reaction includes protease reactions.

61. An integrated system of claim 60 wherein the protease reaction includes protease-K reaction.

62. An integrated system of claim 57 wherein the further reaction includes an amplification reaction.

63. An integrated system of claim 62 wherein the amplification includes SDA.

64. An integrated system of claim 62 wherein the amplification includes PCR.

65. An integrated system of claim 62 wherein the amplification includes linear amplification.

66. An integrated system of claim 62 wherein the amplification includes exponential amplification.

67. An integrated system of claim 45 wherein the power source provides a time varying signal to at least certain of the electrodes.

68. An integrated system of claim 67 wherein the time varying signal includes an AC component.

69. An integrated system of claim 68 wherein the AC component is sinusoidal.

70. An integrated system of claim 67 wherein the time varying signal includes an offset signal.

71. An integrated system of claim 70 wherein the offset is a DC offset.

72. An integrated system of claim 45 further including a control system.

73. An integrated system of claim 72 wherein the control system is a computer.

74. An integrated system of claim 73 wherein the computer is a personal computer.

75. An integrated system of claim 74 wherein the personal computer is a notebook computer.

76. An integrated system of claim 45 wherein the first flow cell of the first electronic system includes at least one port in addition to the input and the output ports.

77. An integrated system of claim 76 wherein the first flow cell of the first electronic system includes at least two ports in addition to the input and the output ports.

78. An integrated system of claim 77 wherein the ports are at substantially 90 degrees to another port.

79. An integrated system of claim 76 wherein at least two of the ports are in opposition to one another.

80. An integrated system of claim 45 further including at least one heater element.

81. An integrated system of claim 80 wherein a heater is disposed on the first electronic system.

82. An integrated system of claim 80 wherein a heater is disposed on the second electronic system.

83. An integrated system of claim 80 wherein the heater element is in thermal contact with either of the first or second electronic systems.

84. An integrated system of claim 45 wherein the detector is a CCD detector.

85. An integrated system of claim 45 wherein the system is a closed system.

86. An integrated system of claim 45 wherein the system is a self contained system.

87. An integrated system of claim 45 wherein the system further includes a fluidic system.

88. An integrated system of claim 87 wherein the fluidic system includes a pump.

89. An integrated system of claim 87 wherein the fluidic system includes a plurality of valves.

90. An integrated system of claim 45 wherein the sample analysis includes binding of molecules to the array selected from the group consisting of hybridization and protein-ligand binding interactions.

91. An integrated system of claim 90 wherein the hybridization is DNA-DNA
hybridization.

92. An integrated system of claim 90 wherein the hybridization is DNA/RNA
hybridization.

93. An integrated system of claim 90 wherein the hybridization is pNA/nucleic acid hybridization.

94. An integrated of claim 90 wherein flue protein-ligand binding is selected from the group consisting of receptor-ligand; antigen-antibody, and enzyme-substrate binding.

95. An integrated system for the analysis of a biological sample containing eukaroytic and/or prokaryotic cells, comprising:
a. a first nonelectronic system for sample preparation, said system further comprising:
at least one input port, a first flow cell connected to the input, a heater element, and at least one output port, said flow cell adapted to receive said sample via the input, said heater being operatively coupled to a power source adapted to heat said flow cell to a temperature sufficient to induce disruption of cell membranes within said sample;
b. a second electronic system for sample analysis, said system further comprising:
at least one input port, a second flow cell connected to the input, a plurality of electrodes positioned within said second flow cell, said electrodes comprising; an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules, and at least one output port, said input of the second electronic system being coupled to at least one output port of the first nonelectronic system;
c. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and d. a power source.

96. An integrated system of claim 95 further comprising an illumination source operatively positioned to provide radiation to the active matrix array.

97. An integrated system of claim 96 wherein the illumination source is a laser.

98. An integrated system of claim 97 wherein the laser is a diode laser.

99. An integrated system of claim 96 wherein the system includes a beam splitter.

100. An integrated system of claim 99 wherein the beam splitter is positioned to receive the illumination and reflect at least a portion thereof to the active matrix array, and to pass at least a portion of the radiation from the active matrix array.

101. An integrated system of claim 95 wherein said plurality of individually addressable electrodes of said electronic system are used as a unit for said analysis.

102. An integrated system of claim 95 wherein said plurality of individually addressable electrodes of said electronic system are used independent of one another in the formation of subsets of electrodes for said analysis.

103. An integrated system of claim 95 wherein the sample preparation includes further reaction steps after said heater induced lysis.

104. An integrated system of claim 103 wherein the further reaction includes enzymatic reaction.

105. An integrated system of claim 103 wherein the further reaction includes chemical reaction.

106. An integrated system of claim 103 wherein the further reaction includes protease reactions.

107. An integrated system of claim 106 wherein the protease reaction includes protease-K reaction.

108. An integrated system of claim 103 wherein the further reaction includes an amplification reaction.

109. An integrated system of claim 108 wherein the amplification includes SDA.

110. An integrated system of claim 108 wherein the amplification includes PCR.

111. An integrated system of claim 108 wherein the amplification includes linear amplification.

112. An integrated system of claim 108 wherein the amplification includes exponential amplification.

113. An integrated system of claim 95 wherein the power source provides a time varying signal to at least certain of the electrodes.

114. An integrated system of claim 113 wherein the time varying signal includes an AC component.

115. An integrated system of claim 114 wherein the AC component is sinusoidal.

116. An integrated system of claim 113 wherein the time varying signal includes an offset signal.

117. An integrated system of claim 116 wherein the offset is a DC offset.

118. An integrated system of claim 9S further including a control system.

119. An integrated system of claim 118 wherein the control system is a computer.

120. An integrated system of claim 119 wherein the computer is a personal computer.

121. An integrated system of claim 120 wherein the personal computer is a notebook computer.

122. An integrated system of claim 95 wherein the first flow cell of the nonelectronic system includes at least one port in addition to the input and the output ports.

123. An integrated system of claim 122 wherein the first flow cell of the nonelectronic system includes at least two ports in addition to the input and the output ports.

124. An integrated system of claim 123 wherein the ports are at substantially degrees to another port.

125. An integrated system of claim 122 wherein at least two of the ports are in opposition to one another.

126. An integrated system of claim 95 further including at least two heater elements.

127. An integrated system of claim 126 wherein a heater is disposed on the electronic system.

128. An integrated system of claim 126 wherein a heater element is in thermal contact with either of the nonelectronic or electronic systems.

129. An integrated system of claim 95 wherein the detector is a CCD detector.

130. An integrated system of claim 95 wherein the system is a closed system.

131. An integrated system of claim 95 wherein the system is a self contained system.

132. An integrated system of claim 95 wherein the system further includes a fluidic system.

133. An integrated system of claim 132 wherein the fluidic system includes a pump.

134. An integrated system of claim 132 wherein the fluidic system includes a plurality of valves.

135. An integrated system of claim 95 wherein the sample analysis includes binding of molecules to the array selected from the group consisting of hybridization and protein-ligand binding interactions.

136. An integrated system of claim 135 wherein the hybridization is DNA-DNA
hybridization.

137. An integrated system of claim 135 wherein the hybridization is DNA/RNA
hybridization.

138. An integrated system of claim 135 wherein the hybridization is pNA/nucleic acid hybridization.

139. An integrated of claim 135 wherein the protein-ligand binding is selected from the group consisting of receptor-ligand, antigen-antibody, and enzyme-substrate binding.