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CA2124580A1 - Method for selecting recombinant microorganisms containing at their surface at least one molecule having enzymatic activity - Google Patents

Method for selecting recombinant microorganisms containing at their surface at least one molecule having enzymatic activity

Info

Publication number
CA2124580A1
CA2124580A1 CA002124580A CA2124580A CA2124580A1 CA 2124580 A1 CA2124580 A1 CA 2124580A1 CA 002124580 A CA002124580 A CA 002124580A CA 2124580 A CA2124580 A CA 2124580A CA 2124580 A1 CA2124580 A1 CA 2124580A1
Authority
CA
Canada
Prior art keywords
molecule
enzymatic activity
recombinant microorganisms
microorganisms containing
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002124580A
Other languages
French (fr)
Other versions
CA2124580C (en
Inventor
Jacques Fastrez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Catholique de Louvain UCL
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2124580A1 publication Critical patent/CA2124580A1/en
Application granted granted Critical
Publication of CA2124580C publication Critical patent/CA2124580C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10051—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

A method for selecting recombinant micro-organisms of which the sur-face comprises at least one molecule having enzymatic activity, wherein the rec-ombinant micro-organisms are immobilized on a solid support by a covalent inhibitor of the active site of said molecule, which is connected to the solid sup-port,by an arm; the recombinant micro-organisms are recovered and all or part of their genome is isolated and cloned.
CA002124580A 1991-11-29 1992-11-30 Method for selecting recombinant microorganisms containing at their surface at least one molecule having enzymatic activity Expired - Lifetime CA2124580C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE9101106 1991-11-29
BE9101106A BE1006312A3 (en) 1991-11-29 1991-11-29 Selection process for recombinant microorganisms with their area at least one molecule enzymatic activity.
PCT/BE1992/000052 WO1993011242A1 (en) 1991-11-29 1992-11-30 Method for selecting recombinant micro-organisms of which the surface comprises at least one molecule having enzymatic activity

Publications (2)

Publication Number Publication Date
CA2124580A1 true CA2124580A1 (en) 1993-06-10
CA2124580C CA2124580C (en) 2002-07-16

Family

ID=3885823

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002124580A Expired - Lifetime CA2124580C (en) 1991-11-29 1992-11-30 Method for selecting recombinant microorganisms containing at their surface at least one molecule having enzymatic activity

Country Status (16)

Country Link
EP (1) EP0617737B1 (en)
JP (1) JP4125364B2 (en)
KR (1) KR100243997B1 (en)
AT (1) ATE147783T1 (en)
AU (1) AU671089B2 (en)
BE (1) BE1006312A3 (en)
BR (1) BR9206970A (en)
CA (1) CA2124580C (en)
DE (1) DE69216853T2 (en)
DK (1) DK0617737T3 (en)
ES (1) ES2098555T3 (en)
FI (1) FI109131B (en)
HU (1) HU220876B1 (en)
NO (1) NO313593B1 (en)
SG (1) SG48094A1 (en)
WO (1) WO1993011242A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL104829A (en) * 1992-02-24 1999-04-11 Igen Inc Reaction-based selection for expression of and concentration of catalytic moieties
WO2000061790A1 (en) * 1999-04-14 2000-10-19 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High sensitivity phage display biomolecule detection

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5325030B2 (en) * 1972-12-19 1978-07-24
US4165258A (en) * 1975-10-08 1979-08-21 University Of Pennsylvania Plasminogen activating enzyme-specific competitive inhibitor
EP0298094A1 (en) * 1986-12-01 1989-01-11 McDONNELL DOUGLAS CORPORATION Method of identifying salmonella
DE3833628A1 (en) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft METHOD FOR DETECTING AND IDENTIFYING TOXIC SUBSTANCES USING CLONED MICROORGANISMS
AU634500B2 (en) * 1988-10-04 1993-02-25 Dna Plant Technology Corporation Bacterial detection by phage transduction of detectable phenotype
JP2810138B2 (en) * 1989-08-23 1998-10-15 昭和電工株式会社 Filler for measuring enzyme activity, method of using the same and apparatus therefor
DE69007006T2 (en) * 1989-09-29 1994-08-25 Rohm & Haas Ligand-containing medium for chromatographic separation, process for its preparation and its use for the isolation of synthetic or natural molecules from a fluid mixture.

Also Published As

Publication number Publication date
DK0617737T3 (en) 1997-07-07
JPH07505999A (en) 1995-07-06
KR100243997B1 (en) 2000-02-01
DE69216853T2 (en) 1997-06-26
SG48094A1 (en) 1998-04-17
FI942469A (en) 1994-05-26
JP4125364B2 (en) 2008-07-30
CA2124580C (en) 2002-07-16
NO941731L (en) 1994-06-21
FI942469A0 (en) 1994-05-26
NO313593B1 (en) 2002-10-28
HU220876B1 (en) 2002-06-29
AU2937392A (en) 1993-06-28
FI109131B (en) 2002-05-31
BR9206970A (en) 1995-12-19
EP0617737B1 (en) 1997-01-15
DE69216853D1 (en) 1997-02-27
ATE147783T1 (en) 1997-02-15
HU9401593D0 (en) 1994-08-29
WO1993011242A1 (en) 1993-06-10
AU671089B2 (en) 1996-08-15
ES2098555T3 (en) 1997-05-01
NO941731D0 (en) 1994-05-10
BE1006312A3 (en) 1994-07-19
HUT70310A (en) 1995-09-28
EP0617737A1 (en) 1994-10-05

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Legal Events

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Effective date: 20121130