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CA2100791A1 - Method of Directing Biosynthesis of Specific Polyketides - Google Patents

Method of Directing Biosynthesis of Specific Polyketides

Info

Publication number
CA2100791A1
CA2100791A1 CA2100791A CA2100791A CA2100791A1 CA 2100791 A1 CA2100791 A1 CA 2100791A1 CA 2100791 A CA2100791 A CA 2100791A CA 2100791 A CA2100791 A CA 2100791A CA 2100791 A1 CA2100791 A1 CA 2100791A1
Authority
CA
Canada
Prior art keywords
polyketide
dna sequence
specific
introducing
biosynthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2100791A
Other languages
French (fr)
Other versions
CA2100791C (en
Inventor
Leonard Katz
Stefano Donadio
James B. Mcalpine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/642,734 external-priority patent/US5824513A/en
Application filed by Individual filed Critical Individual
Priority to PCT/US1992/000427 priority Critical patent/WO1993013663A1/en
Priority to AU12450/92A priority patent/AU665526B2/en
Priority to EP92905082A priority patent/EP0626806A1/en
Priority to CA002100791A priority patent/CA2100791C/en
Priority claimed from PCT/US1992/000427 external-priority patent/WO1993013663A1/en
Publication of CA2100791A1 publication Critical patent/CA2100791A1/en
Application granted granted Critical
Publication of CA2100791C publication Critical patent/CA2100791C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Macromonomer-Based Addition Polymer (AREA)

Abstract

A method to produce novel polyketide structures by designing and introducing specified changes in the DNA governing the synthesis of the polyketide is disclosed. The biosynthesis of specific polyketide analogs is accomplished by genetic manipulation of a polyketide-producing microorganism by isolating a polyketide biosynthetic gene-containing DNA sequence, identifying enzymatic activities associated within the DNA sequence, introducing one or more specified changes into the DNA
sequence which codes for one of the enzymatic activities which results in an altered DNA sequence, introducing the altered DNA sequence into the polyketide-producing microorganism to replace the original sequence, growing a culture of the altered microorganism under conditions suitable for the formation of the specific polyketide analog, and isolating the specific polyketide analog from the culture. The method is most useful when the segment of the chromosome modified is involved in an enzymatic activity associated with polyketide biosynthesis, particularly for manipulating polyketide synthase genes from Saccarharopolyspora or Streptomyces.
CA002100791A 1991-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides Expired - Fee Related CA2100791C (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/US1992/000427 WO1993013663A1 (en) 1992-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides
AU12450/92A AU665526B2 (en) 1991-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides
EP92905082A EP0626806A1 (en) 1992-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides
CA002100791A CA2100791C (en) 1991-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/642,734 US5824513A (en) 1991-01-17 1991-01-17 Recombinant DNA method for producing erythromycin analogs
PCT/US1992/000427 WO1993013663A1 (en) 1992-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides
CA002100791A CA2100791C (en) 1991-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides

Publications (2)

Publication Number Publication Date
CA2100791A1 true CA2100791A1 (en) 1993-07-18
CA2100791C CA2100791C (en) 2000-05-30

Family

ID=25676385

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002100791A Expired - Fee Related CA2100791C (en) 1991-01-17 1992-01-17 Method of directing biosynthesis of specific polyketides

Country Status (2)

Country Link
AU (1) AU665526B2 (en)
CA (1) CA2100791C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6579695B1 (en) 1995-10-13 2003-06-17 President And Fellows Of Harvard College Phosphopantetheinyl transferases and uses thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6579695B1 (en) 1995-10-13 2003-06-17 President And Fellows Of Harvard College Phosphopantetheinyl transferases and uses thereof
US7192735B2 (en) 1995-10-13 2007-03-20 President & Fellows Of Harvard College Phosphopantetheinyl transferases and uses thereof

Also Published As

Publication number Publication date
AU665526B2 (en) 1996-01-11
CA2100791C (en) 2000-05-30
AU1245092A (en) 1993-08-03

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